Publications by authors named "Xiuxiu Hou"

8 Publications

  • Page 1 of 1

Effects of Bleaching Associated with Er:YAG and Nd:YAG Laser on Enamel Structure and Bacterial Biofilm Formation.

Scanning 2021 20;2021:6400605. Epub 2021 Dec 20.

Department of Endodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Objective: To compare the effects of bleaching associated with Er:YAG and Nd:YAG laser on enamel structure and mixed biofilm formation on teeth surfaces.

Materials And Methods: Sixty-eight enamel samples were randomly divided into four groups ( = 17), control, Opalescence Boost only, Opalescence Boost plus Er: YAG laser, and Opalescence Boost plus Nd:YAG laser. The structure was observed using SEM after bleaching. Subsequently, the treated enamel samples were also cultured in suspensions of , , , and () for 24 and 48 h. Biofilm formation was quantified by crystal violet staining, and the structure was visualized by confocal laser scanning microscopy. The data were analyzed using the Kruskal-Wallis method.

Results: The enamel structure significantly changed after bleaching. There was no obvious difference in the biofilm formation after 24 h; however, after 48 hours, the amount of biofilm increased significantly. Remarkably, the amount was significantly higher on enamel bleached only, however, there was no significant difference between samples bleached with Er:YAG or Nd:YAG laser compared to the control.

Conclusions: Bleaching only appeared to markedly promote biofilm formation after 48 h, and the biofilms on samples bleached with Er:YAG or Nd:YAG laser did not change significantly, showing that bleaching with Er:YAG or Nd:YAG laser can be safely applied in clinical practice.
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http://dx.doi.org/10.1155/2021/6400605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712178PMC
January 2022

Pinocembrin relieves hip fracture-induced pain by repressing spinal substance P signaling in aged rats.

J Neurophysiol 2022 Jan 5. Epub 2022 Jan 5.

Department of Traumatology and Orthopedics, Cangzhou Hospital of integrated traditional Chinese medicine and Western medicine, Cangzhou, China.

Whether pinocembrin (PCN) could be utilized to alleviate hip fracture-induced pain is investigated in this research. Aged rats with hip fractures were treated with vehicle or 80 mg/kg/day PCN from week 3 to week 4. Then hind paw mechanical allodynia, unweighting, warmth, and thickness were measured. The microglia and astrocytes activation and proliferation markers in the spinal dorsal horn were detected with real-time PCR and immunofluorescence staining. The relative expression of substance P and its receptor, tachykinin receptor 1 (Tacr1), were detected with enzyme-linked immunosorbent assay (ELISA) and Western blots. The antinociceptive effect of Tacr1 inhibitor LY303870 was also testified. PCN alleviated hip fracture-induced hind paw nociceptive (allodynia and unweighting) and vascular changes (warmth and thickness) in aged rats with diminished microglia and astrocytes activation and proliferation in the spinal dorsal horn. Up-regulated substance P and Tacr1 were induced after hip fracture, which could be reversed by PCN treatment. Furthermore, LY303870 treatment partially reversed both spinal nociceptive sensitization and vascular changes after hip fracture. Substance P signaling contributes to the nociceptive and vascular changes observed in the hip fracture, which could be alleviated by PCN.
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http://dx.doi.org/10.1152/jn.00517.2021DOI Listing
January 2022

Digital analysis of external fixation area of proximal humerus fractures in elderly patients.

BMC Musculoskelet Disord 2021 Nov 26;22(1):991. Epub 2021 Nov 26.

Cangzhou Hospital of integrated traditional Chinese medicine and Western medicine, No.31, West Huanghe Road, Yunhe District, Cangzhou, 061000, Hebei Province, China.

Introduction: The purpose is based on anatomical basis, combined with three-dimensional measurement, to guide the clinical repositioning of proximal humeral fractures, select the appropriate pin entry point and angle, and simulate surgery.

Methods: 11 fresh cadaveric specimens were collected, the distance of the marked points around the shoulder joint was measured anatomically, and the vertical distance between the inferior border of the acromion and the superior border of the axillary nerve, the vertical distance between the apex of the humeral head and the superior border of the axillary nerve, the vertical distance between the inferior border of the acromion and the superior border of the anterior rotator humeral artery, and the vertical distance between the apex of the humeral head and the superior border of the anterior rotator humeral artery were marked on the 3D model based on the anatomical data to find the relative safety zone for pin placement.

Results: Contralateral data can be used to guide the repositioning and fixation of that side of the proximal humerus fracture, and uniform data cannot be used between male and female patients. For lateral pining, the distance of the inferior border of the acromion from the axillary nerve (5.90 ± 0.43) cm, range (5.3-6.9) cm, was selected for pining along the medial axis of the humeral head, close to the medial cervical cortex, and the pining angle was measured in the coronal plane (42.84 ± 2.45)°, range (37.02° ~ 46.31°), and in the sagittal plane (28.24 ± 2.25)°, range (19.22° ~ 28.51°). The pin was advanced laterally in front of the same level of the lateral approach point to form a cross-fixed support with the lateral pin, and the pin angle was measured in the coronal plane (36.14 ± 1.75)°, range (30.32° ~ 39.61°), and in the sagittal plane (28.64 ± 1.37)°, range (22.82° ~ 32.11°). Two pins were taken at the greater humeral tuberosity for fixation, with the proximal pin at an angle (159.26 ± 1.98) to the coronal surface of the humeral stem, range (155.79° ~ 165.08°), and the sagittal angle (161.76 ± 2.15)°, with the pin end between the superior surface of the humeral talus and the inferior surface of the humeral talus. The distal needle of the greater humeral tuberosity was parallel to the proximal approach trajectory, and the needle end was on the inferior surface of the humeral talus.

Conclusion: Based on the anatomical data, we can accurately identify the corresponding bony structures of the proximal humerus and mark the location of the pin on the 3D model for pin placement, which is simple and practical to meet the relevant individual parameters.
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http://dx.doi.org/10.1186/s12891-021-04826-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626931PMC
November 2021

Comparison of three methods of Müller type C2 and C3 distal femoral fracture repair.

J Int Med Res 2021 May;49(5):3000605211015031

North China University of Science and Technology, Tangshan, Hebei province, China.

Background: We compared the outcomes of three fixation techniques for Müller type C2 and C3 distal femoral fractures.

Methods: We retrospectively analyzed patients undergoing internal fixation for Müller type C2 and C3 distal femoral fractures via locking plate (Group A), lateral locking condylar plate and medial contoured reconstruction plate (Group B), and lateral locking condylar plate and anterior reconstruction plate (Group C). Knee joint functional recovery and functional outcomes were evaluated 12 months postoperatively.

Results: Patients included 34 men and 24 women aged 25 to 74 years (mean, 50.3 ± 10.73 years). Operating times were longest in Group B and similar in Groups A and C. Bleeding volume in Group A was smaller than in Group B and similar to that of Group C. Functional outcomes were excellent in 18 (31%) fractures, good in 24 (41%), moderate in 11 (19%), and poor in 5 (9%). Good-to-excellent results were achieved in 56%, 82%, and 83% of patients (Groups A, B, and C, respectively). Groups B and C's outcomes were superior to Group A's outcomes. No significant difference in postoperative complications between the groups existed.

Conclusion: Lateral locking condylar and anterior reconstruction plating was useful for complex type C distal femoral fractures.
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http://dx.doi.org/10.1177/03000605211015031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150468PMC
May 2021

Expression of PIM-1 in salivary gland adenoid cystic carcinoma: Association with tumor progression and patients' prognosis.

Oncol Lett 2018 Jan 14;15(1):1149-1156. Epub 2017 Nov 14.

Department of Head and Neck Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China.

Pim-1 proto-oncogene, serine/threonine kinase (PIM-1) phosphorylates a series of substrates to exert its oncogenic function in numerous malignancies. The present study investigated the clinical significance of the PIM-1 protein, apoptosis status and apoptosis-associated proteins, including forkhead box O3a (FOXO3a), B cell lymphoma-2 (BCL-2) and BCL-2-associted agonist of cell death (BAD), were investigated in salivary gland adenoid cystic carcinoma (ACC) tissues. PIM-1 expression levels in 4 pairs of ACC tissues and corresponding normal salivary gland tissues were determined by western blot analysis. PIM-1, FOXO3a, BAD and BCL-2 expression levels in 60 ACC tissues were evaluated by immunohistochemistry (IHC). A terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay was performed to detect the apoptosis status of ACC tissues. PIM-1 was revealed to be highly expressed in ACC tissues compared with adjacent normal tissues. IHC staining results demonstrated high expression ratios of PIM-1, FOXO3a, BCL-2 and BAD [33.33% (20/60), 51.67% (31/60), 51.67% (31/60) and 55% (33/60)], respectively, and significant correlations between the expression of PIM-1 and FOXO3a and BCL-2 (P<0.05). Apoptotic rates were significantly associated with PIM-1, FOXO3a, BCL-2 and BAD expression levels (P<0.05). PIM-1 expression levels were significantly associated with tumor size, lymph node involvement, nerve invasion, distant metastasis and weakly associated with tumor node metastasis stage. Kaplan-Meier survival curves revealed that PIM-1 expression level was significantly associated with disease-free survival of patients with ACC (P=0.009). Cox regression multivariate analysis results revealed that histotype, distant metastasis and apoptotic rate were independent prognosis factors for ACC. Assessment of PIM-1 may be useful in investigating the malignant behaviors of ACC and predicting the outcome of patients with ACC.
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http://dx.doi.org/10.3892/ol.2017.7408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772885PMC
January 2018

Biochemical changes of salivary gland adenoid cystic carcinoma cells induced by SGI-1776.

Exp Cell Res 2017 03 20;352(2):403-411. Epub 2017 Feb 20.

Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Hangzhou 310022, China. Electronic address:

Provirus integration site for Moloney murine leukemia virus 1 (Pim-1) has proved to be an oncogene and it is known that to depress Pim-1 activity may be a novel oncological treatment strategy. SGI-1776, a small molecule, is the first clinically tested inhibitor of the Pim kinase family. Here, we aimed to explore the effect of SGI-1776 on salivary adenoid cystic carcinoma (SACC). Expression of Pim-1 was confirmed in SACC and control tissues by qRT-PCR. After SGI-1776 treatment, the Pim-1 expressions and Pim-1 kinase activity in both SACC-83 and SACC-LM cell lines were measured. Cell proliferation, cell invasion, cell cycle, apoptosis and mitochondrial membrane potential were analyzed. Also, the expression of FOXO3a, p-FOXO3a, RUNX3, Bcl-2, BAD, p-BAD, Bim and p-Bim were detected by Western blot. The results showed that Pim-1 was significantly overexpressed in SACC tissues. SGI-1776 down-regulated the Pim-1 expression, inhibited Pim-1 kinase activity, reduced cell proliferation, decreased invasive ability, increased caspase-3 activity and induced apoptosis, cell cycle arrest and mitochondrial depolarization. Reduced expression was also seen in p-FOXO3a, RUNX3, Bcl-2, p-BAD and p-Bim, whereas no significant changes were observed from FOXO3a, BAD and Bim. These results confirm the pivotal role of Pim-1 in SACC and suggest that targeting Pim-1 kinase signal pathway by SGI-1776 might be a promising therapeutic modality for SACC.
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http://dx.doi.org/10.1016/j.yexcr.2017.02.029DOI Listing
March 2017

Different Expression of Erythrocyte Sedimentation Rate and C-reactive Protein in Papillary Thyroid Carcinoma and Nodular Goiter.

Clin Lab 2015 ;61(7):793-9

Background: Inflammation is the seventh hallmark of cancer. Growing evidence indicated inflammation was linked to the progression and prognosis of many malignancies. Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), as two inflammation markers, reported frequently in the incidence and progress of solid cancers, but little research has concerned these markers in thyroid disease. In this study, the expression of ESR and CRP both in papillary thyroid carcinoma (PTC) and nodular goiter was investigated.

Methods: 512 patients were recruited to this study, including 341 PTC and 171 nodular goiters. Serum ESR and CRP were tested before operation. The clinical pathological factors such as gender, age, body mass index (BMI), tumor size, extrathyroidal extension, capsule invasion, lymphatic metastasis, distant metastasis, and other thyroid specific markers were subanalyzed in the PTC group.

Results: In patients with PTC, the mean level of ESR was 14.24 ± 11.35 mm/h, which was lower than patients with nodular goiter (16.90 ± 12.00 mm/h, p = 0.006). Meanwhile the mean level of CRP was 1.81 ± 3.51 mg/L in PTC, which was lower than patients with nodular goiter (2.09 ± 3.34 mg/L, p = 0.008). Subanalysis in PTC showed that the ESR/CRP level has no significant difference concerning the capsule invasion, extrathyroidal extension, T grades, lymphatic metastasis, distant metastasis, Tumor Nodulus Metastases (TNM) and tumor sizes (p > 0.05). Pearson's correlation analysis showed a positive correlation between ESR and CRP (r = 0.416, p = 0.000), ESR and Tg (r = 0.140, p = 0.002), CRP and Tg (r = 0.187, p = 0.000). The results of multivariate logistic analysis showed that gender and age were the independent risk factors of ESR, meanwhile age and BMI were the independent risk factors of CRP.

Conclusions: The present study demonstrated a different expression of ESR and CRP between PTC and nodular goiter. Even though the difference in absolute figures between them was very small, it could help clinicians to distinguish the difference between nodular goiter and PTC to some extent. ESR/CRP may have potential value in distinguishing thyroid benign disease from malignant tumors. However, ESR and CRP had no effect on the progress of PTC. They may not have potential value currently in PTC staging and predicting prognosis.
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http://dx.doi.org/10.7754/clin.lab.2015.150127DOI Listing
September 2015

Disparity expression of gammaH2AX in papillary thyroid cancer and nodular goiter.

Clin Lab 2014 ;60(12):2031-7

Background: H2AX, one of the variants of histone H2A with conserved sequence, plays a vital role in maintaining genomic stability. DNA double-strand break (DSB) induces H2AX phosphorylation (γH2AX). Growing evidence indicated γH2AX was linked to thyroid disease. However, the precise expression of γH2AX in papillary thyroid carcinoma (PTC) and nodular goiter was unknown. In this study, the yH2AX expression in PTC and nodular goiter and the significance of the aberrant expression were investigated.

Methods: The γH2AX expression level was evaluated by immunohistochemistry staining in 140 specimens with thyroid diseases (30 cases of nodular goiter, 30 cases of PTC, and 80 cases of PTC coexisting with nodular goiter). The possible relationships between γH2AX expression and patients' clinicopathological characteristics were analyzed.

Results: In the set of nodular goiter, 20.0% (6/30) of nodular goiter component and 10.0% (3/30) of normal adjacent tissues exhibited high expression of γH2AX (p > 0.05). In the set of PTC, 70.0% (21/30) of PTC component and 0.0% (0/30) of normal adjacent tissues showed high expression of γH2AX (p < 0.05). In the set of PTC coexisting with nodular goiter, 95.0% (76/80) of PTC component, 12.5% (10/80) of nodular goiter component, and 8.8% (7/80) of normal adjacent specimens showed high expression of γH2AX (p < 0.05). 85.5% (65/76) of PTC patients with lymph node metastasis were found to have a high expression level of γH2AX, and the proportion was lower than that in patients without lymph node metastasis (34/34, 100.0%) (p = 0.003). High γH2AX expression was also found in 76.2% (16/21) of stage IV patients and the proportion was lower than that in stage I - III patients (83/89, 93.3%) (p = 0.034). No statistically significant correlation was found between yH2AX expression level and other clinicopathological features, including age, gender, capsular invasion, tumor size, tumor focus number, distant metastasis, and T status.

Conclusions: γH2AX expression was increased in PTC patients, while nodular goiter and normal adjacent tissues exhibited no statistically significant difference in γH2AX expression. yH2AX expression had a negative correlation with TNM stage and lymph node metastasis. γH2AX seems to play a more critical role in early-stage PTC rather than late-stage PTC and might inhibit lymph node metastasis.
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http://dx.doi.org/10.7754/clin.lab.2014.140603DOI Listing
March 2015
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