Publications by authors named "Xiuhua Zhang"

138 Publications

A novel indazole derivative, compound Cyy-272, attenuates LPS-induced acute lung injury by inhibiting JNK phosphorylation.

Toxicol Appl Pharmacol 2021 Jul 16:115648. Epub 2021 Jul 16.

Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China; Institute of Chronic Kidney Disease, Wenzhou Medical University, Wenzhou, China. Electronic address:

Acute lung injury (ALI) is a diffuse lung dysfunction disease characterized by high prevalence and high mortality. Thus far, no effective pharmacological treatment has been made for ALI in clinics. Inflammation is critical to the development of ALI. Therefore, anti-inflammation may be a potential therapy strategy for ALI. Indazole-containing derivatives, representing one of the most important heterocycles in drug molecules, are endowed with a broad range of biological properties, such as anti-cancer and anti-inflammation. In the current study, we investigated the biological effects of Cyy-272, a newly synthesized indazole compound, on LPS-induced ALI both in vivo and in vitro. Results show that Cyy-272 can inhibit the release of inflammatory cytokines in LPS-stimulated macrophage and alleviate LPS induced ALI. Further experiment revealed that Cyy-272 exhibit anti-inflammation activity by inhibiting JNK phosphorylation. Overall, our studies show that an indazole derivative, Cyy-272, is effective in suppressing LPS-induced JNK activation and inflammatory signaling.
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http://dx.doi.org/10.1016/j.taap.2021.115648DOI Listing
July 2021

The nature and reported incidence of suspected perioperative allergic reactions: A cross-sectional survey.

J Clin Anesth 2021 Jun 23;74:110404. Epub 2021 Jun 23.

Department of Anesthesiology, Peking Union Medical College Hospital, Beijing 100730, China. Electronic address:

Study Objective: Perioperative allergic reactions (POHs) are common and can lead to severe intraoperative instability and even mortality. In contrast to the situation in developed countries, where databases of perioperative anaphylaxis are well documented and analyzed, relevant data are lacking in China. Therefore, we aimed to conduct a national survey to explore the characteristics of perioperative allergic reactions, as well as the knowledge and attitudes toward management and reporting among anesthesiologists.

Design: Cross-sectional survey.

Setting: Anesthesia department.

Patients: A nationally representative sample comprising anesthesiologists from 12 province-level regions was selected.

Measurements: A 20-item questionnaire was designed and validated using the Delphi method. Survey distribution was performed between June 2019 and January 2020 by the Chinese Society of Anesthesiology (CSA), which is the official academic society of Chinese anesthesiologists. Responses were compiled and analyzed.

Main Results: We received responses from 4389 anesthesiologists across China. The estimated rate of suspected POH was 2/1000 patients (0.2%). On average, an anesthesiologist encountered 2.1 suspected POH cases per year. Neuromuscular blocking agents (NMBAs) were perceived as the most common causative agents, followed by antibiotics and succinylated gelatin. The rates of referral and allergy consultations were very low. Institutional support, including protocol development, cognitive aids, and tool kits, was not ideal. Additionally, the management of POH varied substantially. Most anesthesiologists believed that reporting and documenting POH was necessary.

Conclusions: Our survey revealed that POH is commonly encountered by Chinese anesthesiologists, but few patients are referred to allergy specialists or clinics for further investigation. A standardized recommendation based on research and data derived from Chinese patients is required.
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http://dx.doi.org/10.1016/j.jclinane.2021.110404DOI Listing
June 2021

Simple MoS-Nanofiber Paper-Based Fluorescence Immunosensor for Point-of-Care Detection of Programmed Cell Death Protein 1.

Anal Chem 2021 06 14;93(25):8791-8798. Epub 2021 Jun 14.

Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, P. R. China.

Programmed cell death protein 1 (PD-1) is one of the coinhibitory checkpoints upon T cell activation, the abnormal expression of which severely threatens host immune modulatation for chronic infection. Thus, fast and sensitive monitoring of PD-1 is of vital importance for early diagnosis and cancer treatment. The current detection methods largely based on enzyme-linked immunosorbent assay (ELISA) require time-consuming incubation and complicated washing steps. Herein, we designed a simple and portable nanofiber paper (NFP)-based fluorescence "off-on" immunosensor for PD-1 rapid determination. Molybdenum disulfide (MoS) nanosheets modified NFP (MoS-NFP) was employed for adsorbing and immobilizing CdSe/ZnS quantum dots-antibody (QDs-Ab) complex to construct a ready-to-use fluorescent immunosensor. The fluorescent signal of QDs-Ab was initially quenched by MoS under the Förster resonance energy transfer (FRET) effect. When the PD-1 target was specifically captured onto NFP by immunization, the QDs-Ab-PD-1 complex was promptly desorbed from the MoS-NFP surface, resulting in FRET impediment and fluorescence recovery. As an alternative quenching agent, graphene oxide (GO) served as a contrast to investigate NFP-based sensing performance. Owing to superior quenching and desorption efficiency, the MoS-NFP-based fluorescence immunosensor exhibited nearly 2-fold lower detection limit (85.5 pg/mL) than GO-NFP-based sensor (151 pg/mL) for PD-1 monitoring. Excellent selectivity and satisfactory recovery in PD-1 mouse cell culture supernatant samples were confirmed as well. In addition, the comparable detectability of the MoS-NFP-based immunosensor was accurately evaluated by a standard PD-1 mouse ELISA kit. This study displayed a simple, rapid, low-cost, and portable point-of-care PD-1 assay, indicating its broad application prospect toward clinical diagnoses.
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http://dx.doi.org/10.1021/acs.analchem.1c00269DOI Listing
June 2021

Lack of Spinal Neuropeptide Y Is Involved in Mechanical Itch in Aged Mice.

Front Aging Neurosci 2021 28;13:654761. Epub 2021 May 28.

Department of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

Neuropeptide Y (NPY) signaling plays an essential role in gating the pruritic afferent information in the spinal cord. Recent studies revealed that the aging process down-regulated the expression of NPY in the central nervous system. We propose that the lack of spinal NPY may be involved in certain types of pruritus in the elderly population. This study was designed to investigate the role of NPY in aging-induced itch using the senile mouse model. The expression of NPY in the spinal dorsal horn was compared between young (2 months old) and aged (24 months old) mice. Western blotting and immunohistochemistry showed that the expression of NPY was significantly reduced in the spinal dorsal horn in aged mice. In addition, a neuronal maker of apoptosis, TUNEL, was detected in the NPY positive neurons only in the aged spinal cord. Behavioral assay indicated that light mechanical stimulus evoked significantly more scratching in the aged than in the young mice, whereas chemical-evoked itch and pain-related behaviors were not altered. Intrathecal injection of either NPY or LP-NPY, a NPY receptor 1 (NPY1R) agonist, significantly alleviated the mechanically evoked itch in aged mice without altering the responses to chemical pruritogens. Our study suggested that downregulation of spinal NPY in the aged mice might play a role in the higher incidence of the mechanically evoked itch than that in the young mice. Therapies targeting the NPY system might serve as a potential strategy for alleviating the pruritic symptoms among the elderly population.
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http://dx.doi.org/10.3389/fnagi.2021.654761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192807PMC
May 2021

Primary tumor resection improves survival in patients with multifocal intrahepatic cholangiocarcinoma based on a population study.

Sci Rep 2021 Jun 9;11(1):12166. Epub 2021 Jun 9.

Medical Center for Digestive Diseases, Second Affiliated Hospital, Nanjing Medical University, Nanjing, China.

The purpose of our study was to evaluate the effect of surgery on the survival and prognosis of patients with multifocal intrahepatic cholangiocarcinoma (ICCA). Patients with multifocal ICCA were selected from the SEER (Surveillance, Epidemiology, and End Results) database between 2010 and 2016. Kaplan-Meier analyses and log-rank tests were used to evaluate the difference in survival between the surgery group and the non-surgery group. We applied the Cox proportional hazards regression model to identify prognostic factors of overall survival (OS) and cancer-specific survival (CSS). In total, 580 patients were enrolled in our study, including 151 patients who underwent surgery and 429 patients who did not. The median survival time of surgical patients was longer than non-surgical patients (OS: 25 months vs. 8 months, p < 0.001; CSS: 40 months vs. 25 months, p < 0.001). Similarly, the 5-year survival rate in the surgery group was significantly higher than those in the non-surgery group (5-year OS rate: 12.91% vs. 0%; p < 0.001; 5-year CSS rate:26.91% vs. 0%; p < 0.001). Multivariate Cox analysis showed that the OS (HR:0.299, 95% CI: 0.229-0.390, p < 0.001) and CSS (HR:0.305, 95% CI:0.222-0.419, p < 0.001) of patients undergoing surgical resection were significantly improved. Meanwhile, after propensity score matching (PSM) of the original data, we come to the same conclusion.
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http://dx.doi.org/10.1038/s41598-021-91823-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190174PMC
June 2021

Single-Particle Electrochemical Biosensor with DNA Walker Amplification for Ultrasensitive HIV-DNA Counting.

Anal Chem 2021 03 7;93(10):4506-4512. Epub 2021 Mar 7.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, P. R. China.

Single-particle electrochemical collision has gained great achievements in fundamental research, but it is challenging to use in practice on account of its low collision frequency and the interference of the complex matrix in actual samples. Here, magnetic separation and DNA walker amplification were integrated to build a robust and sensitive single-particle electrochemical biosensor. Magnetic nanobeads (MBs) can specifically capture and separate targets from complex samples, which not only ensures the anti-interference capability of this method but also avoids the aggregation of platinum nanoparticles (Pt NPs) caused by numerous coexisting substances. A low amount of targets can lead to the release of more Pt NPs and the generation of more collision current transients, realizing cyclic amplification. Compared with simple hybridization, a DNA walker can improve the collision frequency by about 3-fold, greatly enhancing detection sensitivity, and a relationship between collision frequency and target concentration is used to realize quantification. The biosensor realized an ultrasensitive detection of 4.86 fM human immunodeficiency virus DNA (HIV-DNA), which is 1-4 orders of magnitude lower than that of traditional methods. The successful HIV-DNA detection in complex systems (serum and urine) demonstrated a great promising application in real samples and in the development of new single-entity biosensors.
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http://dx.doi.org/10.1021/acs.analchem.0c04861DOI Listing
March 2021

Suppression of TLR4-MyD88 signaling pathway attenuated chronic mechanical pain in a rat model of endometriosis.

J Neuroinflammation 2021 Mar 5;18(1):65. Epub 2021 Mar 5.

Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.

Background: As a classic innate immunity pathway, Toll-like receptor 4 (TLR4) signaling has been intensively investigated for its function of pathogen recognition. The receptor is located not only on immune cells but also on sensory neurons and spinal glia. Recent studies revealed the involvement of neuronal TLR4 in different types of pain. However, the specific role of TLR4 signaling in the pain symptom of endometriosis (EM) remains obscure.

Methods: The rat endometriosis model was established by transplanting uterine horn tissue into gastrocnemius. Western blotting and/or immunofluorescent staining were applied to detect high mobility group box 1 (HMGB1), TLR4, myeloid differentiation factor-88 adaptor protein (MyD88), and nuclear factor kappa-B-p65 (NF-κB-p65) expression, as well as the activation of astrocyte and microglia. The antagonist of TLR4 (LPS-RS-Ultra, LRU) and MyD88 homodimerization inhibitory peptide (MIP) were intrathecally administrated to assess the behavioral effects of blocking TLR4 signaling on endometriosis-related pain.

Results: Mechanical hyperalgesia was observed at the graft site, while HMGB1 was upregulated in the implanted uterine tissue, dorsal root ganglion (DRG), and spinal dorsal horn (SDH). Compared with sham group, upregulated TLR4, MyD88, and phosphorylated NF-κB-p65 were detected in the DRG and SDH in EM rats. The activation of astrocytes and microglia in the SDH was also confirmed in EM rats. Intrathecal application of LRU and MIP alleviated mechanical pain on the graft site of EM rats, with decreased phosphorylation of NF-κB-p65 in the DRG and reduced activation of glia in the SDH.

Conclusions: HMGB1-TLR4-MyD88 signaling pathway in the DRG and SDH may involve in endometriosis-related hyperpathia. Blockade of TLR4 and MyD88 might serve as a potential treatment for pain in endometriosis.
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http://dx.doi.org/10.1186/s12974-020-02066-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934423PMC
March 2021

Analysis of the risk factors of linezolid-related haematological toxicity in Chinese patients.

J Clin Pharm Ther 2021 Jun 8;46(3):807-813. Epub 2021 Feb 8.

Department of National Drug Cinical Trial Institute, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

What Is Known And Objectives: Haematological toxicity including thrombocytopenia, anaemia and leucopenia is the main adverse events of linezolid (LZD) therapy. This study aimed to investigate the risk factors for LZD-induced haematological toxicity and define the threshold of plasma trough concentration to minimize the haematological toxicity.

Methods: 145 patients who received LZD for more than 10 days were retrospectively reviewed to determine the incidence of LZD-induced haematological toxicity. Meanwhile, the risk factors of haematological toxicity were confirmed by univariate and multivariate logistic regression analysis.

Results And Discussion: 9 (6.2%) patients developed leucopenia, while 52 (35.9%) and 26 (17.9%) patients developed thrombocytopenia and anaemia, respectively. The estimated glomerular filtration rate (eGFR) <90 ml/min/1.73 m (OR, 2.744; 95% CI, 1.117-6.734; p = 0.028) and baseline platelet count <200 × 10 /L (OR, 6.817; 95% CI, 2.870-16.193; p < 0.0001) were found to be significant risk factors for LZD-related thrombocytopenia. Aspartate aminotransferase (AST) >80 U/L (OR, 4.844; 95% CI, 1.207-19.451; p = 0.026) and eGFR <90 ml/min/1.73 m (OR, 7.132; 95% CI, 2.088-24.357; p = 0.002) were the risk factors for LZD-related anaemia. However, no significant risk factors were identified for LZD-related leucopenia. Moreover, LZD plasma trough concentration >8 mg/L [OR, 3.047; 95% CI, 1.233-7.539; p = 0.016] could be a predictor for the development of thrombocytopenia and anaemia.

What Is New And Conclusion: Hepatic and/or renal dysfunction are the risk factors for LZD-related haematological toxicity, while the target plasma trough concentration within 8 mg/L via dose reduction could minimize the haematological toxicity induced by LZD.
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http://dx.doi.org/10.1111/jcpt.13359DOI Listing
June 2021

Composition and risk assessment of perioperative patient safety incidents reported by anesthesiologists from 2009 to 2019: a single-center retrospective cohort study.

BMC Anesthesiol 2021 01 7;21(1). Epub 2021 Jan 7.

Department of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shuaifuyuan 1#, Dongcheng District, 100730, Beijing, China.

Background: Patient safety incident (PSI) reporting has been an important means of improving patient safety and enhancing organizational quality control. Reports of anesthesia-related incidents are of great value for analysis to improve perioperative patient safety. However, the utilization of incident data is far from sufficient, especially in developing countries such as China.

Methods: All PSIs reported by anesthesiologists in a Chinese academic hospital between September 2009 and August 2019 were collected from the incident reporting system. We reviewed the freeform text reports, supplemented with information from the patient medical record system. Composition analysis and risk assessment were performed.

Results: In total, 847 PSIs were voluntarily reported by anesthesiologists during the study period among 452,974 anesthetic procedures, with a reported incidence of 0.17%. Patients with a worse ASA physical status were more likely to be involved in a PSI. The most common type of incident was related to the airway (N = 208, 27%), followed by the heart, brain and vascular system (N = 99, 13%) and pharmacological incidents (N = 79, 10%). Those preventable incidents with extreme or high risk were identified through risk assessment to serve as a reference for the implementation of more standard operating procedures by the department.

Conclusions: This study describes the characteristics of 847 PSIs voluntarily reported by anesthesiologists within eleven years in a Chinese academic hospital. Airway incidents constitute the majority of incidents reported by anesthesiologists. Underreporting is common in China, and the importance of summarizing and utilizing anesthesia incident data should be scrutinized.
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http://dx.doi.org/10.1186/s12871-020-01226-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789294PMC
January 2021

Cu-modified hollow carbon nanospheres: an unusual nanozyme with enhanced peroxidase-like activity.

Mikrochim Acta 2021 Jan 2;188(1). Epub 2021 Jan 2.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, People's Republic of China.

A Cu-modified carboxylated hollow carbon nanospheres (Cu-HCNSs-COOH) was designed with enhanced peroxidase-like activity for the detection of hydrogen peroxide (HO) and degradation of methylene blue (MB). Hollow polymer nanospheres were fabricated from aniline, pyrrole, Triton-100, and ammonium persulfate via confined interfacial copolymerization reaction, which can be pyrolyzed to create HCNSs with the hollow gap diameter of about 20 nm under high temperature. Combining the synergistic effect of coordination and electrostatic interaction, Cu-HCNSs-COOH was constructed by anchoring Cu on the surface of HCNSs-COOH. Furthermore, Cu-HCNSs-COOH has higher affinity for 3,3',5,5'-tetramethylbenzidine and HO of 0.20 mM and 0.88 mM, respectively. Based on the rapid response of Cu-HCNSs-COOH to HO, we constructed a colorimetric sensing platform by detecting the absorbance of the 3,3',5,5'-tetramethylbenzidine-HO system at 652 nm for quantifying HO, which holds good linear relationship between 1 and 150 μM and has a detection limit of 0.61 μM. We also investigated the degradation of MB in the presence of Cu-HCNSs-COOH and HO, which can degrade 80.7% pollutants within 30 min. This research developed an unusual nanozyme for bioassays and water pollution treatment, which broadened the way for the rapid development of clinical diagnostics and water pollution treatment.
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http://dx.doi.org/10.1007/s00604-020-04690-0DOI Listing
January 2021

Visible light mediated self-powered sensing based on target induced recombination of photogenerated carriers.

J Hazard Mater 2021 04 3;407:124765. Epub 2020 Dec 3.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China. Electronic address:

Self-powered sensing platforms have received widespread attention in areas such as portable, wearable and point-of-care devices. Here we reported visible light mediated self-powered electrochemical sensing based on target induced recombination of photogenerated carriers, which has highly sensitive to detect copper ions concentration. We utilized the recombination of photogenerated carriers mechanism to design visible light-responsive FeO-CdS n-n heterojunction as photoanode material, which greatly improved the problem of output energy in photocatalytic self-powered sensors. Expectedly, our proposed visible light mediated self-powered electrochemical system has high separation efficiency of photogenerated carriers, which is 8.4 times that in presence of Cu. Furthermore, this self-powered electrochemical sensing platform used Cu induced recombination of photogenerated carriers, showed a clear linear relationship from 1 nM to 5000 nM with an acceptable detection limit of 0.4 nM. This self-powered electrochemical sensing platform with excellent selectivity, accredited reproducibility and believable stability exhibited promising prospects in developing portable sensing devices and detection chip for real-time and rapid monitoring of Cu.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124765DOI Listing
April 2021

Magnetic Nanobeads and De Novo Growth of Electroactive Polymers for Ultrasensitive microRNA Detection at the Cellular Level.

Anal Chem 2021 01 7;93(2):902-910. Epub 2020 Dec 7.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, P. R. China.

Detection of biomarkers at the cellular level can provide more accurate and comprehensive information that is important for early diagnosis of diseases and evaluation of new drugs. However, the interference of a large number of components in cells and the requirement of high sensitivity bring great challenges for their detection. Herein, a robust and enzyme-free electrochemical platform was proposed for microRNA-21 (miRNA-21) detection by integrating the efficient separation of magnetic nanobeads (MBs) with the multisignal amplification of strand displacement amplification (SDA) and electrochemically mediated atom transfer radical polymerization (eATRP). The eATRP is capable of de novo growth of a number of electroactive polymers on the electrode surface for signal amplification. Compared to simple hybridization, SDA and eATRP can enhance the signals by ∼35-fold, achieving high signal-to-noise ratio for low-abundant target detection. Owing to their superparamagnetism and strong magnetic response ability, MBs endow the method with excellent specificity and anti-interference ability to detect miRNA-21 in cells. Using MBs as capture carriers, SDA and eATRP for signal amplification, and gold nanoflower (AuNF)-modified electrodes as working electrodes, as low as 0.32 aM miRNA-21 was detected. Furthermore, the successful detection of miRNA-21 in cells indicated the great prospect of this method in the early diagnosis of cancers, life science research, and single-entity electrochemical detection.
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http://dx.doi.org/10.1021/acs.analchem.0c03558DOI Listing
January 2021

Primary Tumor Resection Improves Survival in Patients With Metastatic Gastrointestinal Stromal Tumors: A Preliminary Population-Based Analysis.

Front Oncol 2020 19;10:1440. Epub 2020 Aug 19.

Medical Centre for Digestive Diseases, Second Affiliated Hospital, Nanjing Medical University, Nanjing, China.

Surgery has been the primary treatment in patients with localized gastrointestinal stromal tumors (GISTs) for many decades, whereas it remains controversial regarding the efficacy of primary tumor resection for metastatic GISTs treated with chemotherapy, and likewise it is unclear who would benefit from the surgical resection. GISTs patients with distant metastases were identified from the Surveillance, Epidemiology, and End Results (SEER) database between 2010 and 2016. Cox proportional hazards regression models were used to identify prognostic factors of overall survival (OS) and cancer-specific survival (CSS). Kaplan-Meier analyses and log-rank tests were conducted to assess the effectiveness of surgery on survival. In total, of 455 patients with metastatic GISTs, 235 patients (51.6%) underwent primary tumor resection and 220 patients (48.4%) did not. Median survival of patients in resection group was 72 (95% CI: 62.90-81.10) months vs. 40 (95% CI: 29.53-50.47) months for those in non-resection group ( < 0.001). Similarly, surgery in conjunction with chemotherapy led to a favorable impact on survival than chemotherapy alone (OS: 72 vs. 40 months, < 0.001; CSS: 74 vs. 44 months, < 0.001). Multivariable analysis showed that both OS (HR: 0.581, 95% CI: 0.386-0.874, = 0.009) and CSS (HR: 0.663, 95% CI: 0.439-0.912, = 0.042] were dramatically improved in patients with surgical removal of primary site, as well as primary tumor size between 5 and 10 cm, while increasing age was predictive of poorer survival. Stratified analysis revealed that patients with tumor locations in the stomach demonstrated a prolonged survival after surgery, with no significant differential surgical effect between the stomach and small intestine. Our study preliminarily suggests that carefully selected patients with metastatic GISTs might prolong survival after treatment of surgery, especially those with a primary tumor between 5 and 10 cm and a tumor located in the stomach.
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http://dx.doi.org/10.3389/fonc.2020.01440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466430PMC
August 2020

An ANP-fuzzy evaluation model of food quality safety supervision based on China's data.

Food Sci Nutr 2020 Jul 5;8(7):3157-3163. Epub 2020 Jun 5.

School of Economics and Management Nanjing Tech University Nanjing China.

A government acts as the main supervisor of food quality and safety. How to quantify and improve the performance and efficiency of government supervision, respectively, is an urgent problem. This study constructs a food safety supervision performance index and utilizes an analytic network process-fuzzy comprehensive evaluation model to precisely quantify the performance of government supervision. The evaluation results show that, (a) although the overall situation of food quality and safety in China is controllable, the government does not do well in food safety risk supervision, food recall supervision, and accident summary supervision. (b) Internal supervision is the weakest link in food quality and safety supervision. (c) Grassroots supervision is weak especially in prefecture and county levels. (d) There is no positive correlation between the economy level and supervision level in one region. This paper contributes to accurately reflecting the status quo of China's food safety supervision and realizing the transparency of government regulatory information, which ultimately boosts the government's efficiency in food safety supervision and improves the regulatory situation.
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http://dx.doi.org/10.1002/fsn3.1561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382133PMC
July 2020

In Vitro Dissolution and In Vivo Bioequivalence Evaluation of Two Metformin Extended-Release Tablets.

Clin Pharmacol Drug Dev 2021 Apr 24;10(4):414-419. Epub 2020 Jul 24.

Office of Drug Clinical Trial Institution, First Affiliated Hospital of Wenzhou Medical University, Zhejiang, China.

The objective of the present study was to evaluate the bioequivalence between generic and branded metformin extended-release (ER) tablets in Chinese subjects. We tested bioequivalence in vitro and in vivo using a comparative dissolution study and a comparative pharmacokinetic trial. Safety assessments were conducted throughout the entire trial period. The dissolution profiles of the generic formulation expressed obvious extended-release properties, similar to those of the branded formulation (f > 60.0%). Consistent with the result of the in vitro study, no remarkable differences were found in terms of pharmacokinetic profiles between generic and branded formulations. The 90% confidence intervals of Ln AUC , Ln AUC , and Ln C from generic formulation versus branded formulation were 91.4% to 105.0%, 91.3% to 104.7%, and 101.2% to 119.4%, respectively. During the entire trial period, 4 subjects experienced 11 adverse events. All these were mild and spontaneously resolved. The results obtained from the present study suggest that the generic and branded metformin ER tablets were bioequivalent in Chinese subjects.
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http://dx.doi.org/10.1002/cpdd.857DOI Listing
April 2021

A novel solution-gated graphene transistor biosensor for ultrasensitive detection of trinucleotide repeats.

Analyst 2020 Jul 1;145(14):4795-4805. Epub 2020 Jul 1.

Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, No. 368 Youyi Avenue, Wuchang, Wuhan 430062, China.

A new way to detect GAA trinucleotide repeats (TNRs) based on a solution-gated graphene transistor (SGGT) with high performance was developed. Friedreich's ataxia (FRDA) is a neurodegenerative disease where the first intron of the frataxin (FXN) gene exhibits an extended GAA repeat region. Herein, a SGGT biosensor was constructed based on G-quadruplex DNAzymes and graphene channels. The DNAzymes quantify the captured target DNA by producing a strong catalytic current signal depending on the peroxidase-like activity. The higher the target DNA quantity captured on the gate electrode is, the higher is the concentration of DNAzymes on the surface of the gate electrode, which generates a high catalytic current. Due to the excellent self-amplifying performance of the transistor, the current signal of the SGGT is several hundreds of times larger than in conventional electrochemistry under identical detection conditions. Moreover, a large current signal can be obtained in the case of a low concentration of HO when compared to the case of an enzyme-catalyzed transistor. The SGGT biosensor also exhibits an ultra-low detection limit (32.25 fM), a wide linear range (100 fM-100 nM), and excellent selectivity. The results show that the SGGT biosensor has great potential in the early diagnosis of neurodegenerative diseases.
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http://dx.doi.org/10.1039/d0an00205dDOI Listing
July 2020

Predictors of hemodynamic instability in patients with pheochromocytoma and paraganglioma.

J Surg Oncol 2020 Jun 20. Epub 2020 Jun 20.

Department of Anesthesiology, Peking Union Medical College Hospital, Beijing, China.

Background And Objectives: Intraoperative hemodynamic instability is common in patients with pheochromocytoma and paraganglioma. The aim of this study was to identify the predictive risk factors of intraoperative hemodynamic instability.

Methods: A total of 428 patients having elective resection of pheochromocytoma and/or paraganglioma at Peking Union Medical College Hospital between January 2014 and July 2019 were included. The association between preoperative parameters and the incidence of intraoperative hemodynamic instability were evaluated. Binary logistic regression was used to assess the predictive risk factors of hemodynamic instability.

Results: Patients with intraoperative hemodynamic instability were more prone to have elevated levels of norepinephrine and epinephrine. Binary Logistic regression showed the risk factors of hemodynamic instability were tumor size >5.0 cm (odds ratio [OR], 1.889; 95% confidence interval [CI], 1.243-2.870; P = .003) and five-fold increases of urine epinephrine (OR, 2.195; 95% CI, 1.242-3.880; P = .007).

Conclusions: Intraoperative hemodynamic instability is common despite adequate preoperative medical treatment. Tumor size and high level of urinary epinephrine are tumor-related factors for intraoperative hemodynamic instability. Identifying these factors can help clinicians to manage patients more effectively and improve patients' outcomes.
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http://dx.doi.org/10.1002/jso.26079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496938PMC
June 2020

Iron doped graphitic carbon nitride with peroxidase like activity for colorimetric detection of sarcosine and hydrogen peroxide.

Mikrochim Acta 2020 06 12;187(7):383. Epub 2020 Jun 12.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, People's Republic of China.

The successful synthesis is reported of Mn, Fe, Co, Ni, Cu-doped g-CN nanoflakes via a simple one-step pyrolysis method, respectively. Among them, the Fe-doped g-CN nanoflakes exhibited the highest peroxidase-like activity, which can be used for colorimetric detection of hydrogen peroxide (HO) and sarcosine (SA), within the detection ranges of 2-100 μM and 10-500 μM and detection limits of 1.8 μM and 8.6 μM, respectively. The catalytic mechanism of the Fe-doped g-CN nanoflake was also explored and verified the generation of hydroxyl radical (•OH) through fluorescence method. It is believed that the Fe-doped g-CN nanoflakes as enzyme mimics will greatly promote the practical applications in a variety of fields in the future including biomedical science, environmental governance, antibacterial agent, and bioimaging due to their extraordinary catalytic performance and stability. Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04373-wDOI Listing
June 2020

A HO-free electrochemical peptide biosensor based on [email protected] bimetallic nanorods for highly sensitive sensing of matrix metalloproteinase 2.

Chem Commun (Camb) 2020 Jun 11;56(45):6039-6042. Epub 2020 May 11.

State Key Laboratory of Biocatalysis and Enzyme Engineering & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, People's Republic of China.

Inspired by the excellent catalytic activity of [email protected] bimetallic nanorods, we construct a HO-free electrochemical peptide biosensor based on [email protected] bimetallic nanorods for highly efficient and sensitive sensing of MMP-2 for the first time, not only simplifying the traditional testing steps but also avoiding the potential damage caused by HO to peptides and proteins.
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http://dx.doi.org/10.1039/d0cc01598aDOI Listing
June 2020

A competitive self-powered sensing platform based on a visible light assisted zinc-air battery system.

Chem Commun (Camb) 2020 May 9;56(43):5739-5742. Epub 2020 May 9.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, People's Republic of China.

We developed a novel competitive self-powered sensing platform based on the discharge process of a visible light assisted zinc-air battery system for the detection of targets with a high open circuit potential signal (E = 1.5 V), which is a splendid signal among state-of-the-art self-powered sensing platforms.
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http://dx.doi.org/10.1039/d0cc01163kDOI Listing
May 2020

Pediatric Patients with Previous Anaphylactic Reactions to General Anesthesia: a Review of Literature, Case Report, and Anesthetic Considerations.

Curr Allergy Asthma Rep 2020 04 22;20(6):15. Epub 2020 Apr 22.

Department of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, No.1 Shuaifuyuan, Dongcheng District, Beijing, 100730, China.

Purpose Of Review: It is rare to see pediatric patients with previous perioperative anaphylaxis receiving future anesthesia, but it is critical to understand how to choose assessments, interpret the results, and develop a future anesthetic plan.

Recent Findings: Analysis of the results revealed that patients, at any age, regardless of sex and nationality, and the number of surgeries, have the risk of perioperative anaphylaxis while the risk of allergy increases as patients present multiple surgical events or have a previous history of atopy. 94.7% of pediatric patients with allergy testing after perioperative anaphylaxis tolerated subsequent general anesthesia without complications. Specific IgE tests, basophil activation tests, and skin tests are not available and suitable for all culprits. The early skin test could be considered a supplement for later testing. Drug challenge test is the golden standard but can only be used as the last resort. If general anesthesia is inevitable, avoidance of the culprit and use of alternative agents can help the patients prevent another potential recurrence. Full use of inhalation anesthesia without unnecessary neuromuscular blockade agents and avoidance of latex is recommended when the surgery is urgent or skin tests for children cannot be performed in time. This review summarizes characteristics of perioperative pediatric anaphylaxis, main tests for various drugs, and their sensitivities and specificities as well as recommendations as to how to implement safe anesthesia in the future.
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http://dx.doi.org/10.1007/s11882-020-00911-xDOI Listing
April 2020

Synthesis of [email protected] nanospheres for efficient near-infrared radiation mediated treatment and care of gastric cancer patients.

J Photochem Photobiol B 2020 Mar 4;206:111849. Epub 2020 Mar 4.

Department of Oncology, Linyi Cancer Hospital, Shandong Province, China. Electronic address:

Rational design and fabrication of suitable optical absorbing agents (OAAs) are of great significance for state-of-the-art photothermal cancer therapy. Copper selenides have recently emerged as a type of promising photothermal operators thanks to their engineered effortlessness, high carrier concentration, and superior photothermal transformation. Nevertheless, the further in vivo applications are hampered owing to their poor biocompatibility and hydrophobicity. Herein, we synthesized the judicious structure of CuSe nanospheres coated with mesoporous silica for improving their biocompatibility and hydrophilicity. The [email protected] core-shell nanospheres were studied by scanning/transmission electron microscopy (SEM/TEM), X-ray (XRD and XPS), and UV-Visible techniques. Such nanocomposite achieved great ability in photothermal removal of cancer cells with little toxicity. The cell death mechanism has been investigated through typical biochemical assays including acridine orange and ethidium bromide (AO-EB), flowcytometry, and nuclear staining. In addition, the cell cycle arrest in human gastric cancer cells (GCCs) has also been evaluated. Strikingly, the in vivo chemo-photothermal therapy of as-prepared [email protected] toward GCCs confirms the excellent antitumor activity of the core-shell nanospheres under near-infrared radiation (NIR) radiation. It is firmly believed that the [email protected] nanocomposites hold great potential to function as OAAs for NIR mediated treatment and care of gastric cancer patients.
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http://dx.doi.org/10.1016/j.jphotobiol.2020.111849DOI Listing
March 2020

Prognostic and clinicopathological significance of SNHG6 in human cancers: a meta-analysis.

BMC Cancer 2020 Jan 30;20(1):77. Epub 2020 Jan 30.

Medical Centre for Digestive Diseases, Second Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, People's Republic of China.

Background: Recently, accumulating evidence has suggested that the aberrant expression of SNHG6 exists in a variety of tumors and has a correlation with poor clinical outcomes across cancer patients. Considering the inconsistent data among published studies, we aim to assess the prognostic effect of SNHG6 on malignancies.

Methods: We retrieved relevant publications in Web of Science, Embase, MEDLINE, PubMed and Cochrane Library based on predefined selection criteria, up to October 1, 2019. Pooled hazard ratios (HRs) and odds ratios (ORs) with 95% confidence intervals (CIs) were utilized to evaluate the correlation between SNHG6 and overall survival (OS), recurrence-free survival (RFS) and progression-free survival (PFS) as well as clinicopathology.

Results: In total, 999 patients from 14 articles were enrolled in our meta-analysis. The results revealed that augmented SNHG6 expression was significantly correlated with poor OS (HR = 2.20, 95% CI = 1.76-2.75, P < 0.001) and RFS (HR = 3.10, 95% CI = 1.90-5.07, P < 0.001), but not with PFS (HR = 2.11, 95% CI = 0.82-5.39, P = 0.120). In addition to lung cancer and ovarian cancer, subgroup analysis showed that the prognostic value of SNHG6 across multiple tumors was constant as the tumor type, sample size, and methods of data extraction changed. Moreover, cancer patients with enhanced SNHG6 expression were prone to advanced TNM stage (OR = 3.31, 95% CI = 2.46-4.45, P < 0.001), distant metastasis (OR = 4.67, 95% CI = 2.98-7.31, P < 0.001), lymph node metastasis (OR = 2.59, 95% CI = 1.41-4.77, P = 0.002) and deep tumor invasion (OR = 3.75, 95% CI = 2.10-6.69, P < 0.001), but not associated with gender, histological grade and tumor size.

Conclusions: SNHG6 may serve as a promising indicator in the prediction of prognosis and clinicopathological features in patients with different kinds of tumors.
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http://dx.doi.org/10.1186/s12885-020-6530-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993398PMC
January 2020

Target-Driven Cascade-Amplified Release of Loads from DNA-Gated Metal-Organic Frameworks for Electrochemical Detection of Cancer Biomarker.

ACS Appl Mater Interfaces 2020 Jan 31;12(2):2087-2094. Epub 2019 Dec 31.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering , Hubei University , Wuhan 430062 , PR China.

In this paper, a label-free and stimuli-responsive electrochemical biosensing platform was fabricated based on target-driven load release from DNA-gated metal-organic frameworks (MOFs) with cascade amplification. By using MOFs (UiO-66-NH) as a nanocarrier of electroactive molecules (methylene blue; MB) and the programmably assembled DNA acted as the gatekeeper, the biofunctionalized MOFs ([email protected]/MOFs) were not only used as an amplified signal label but also worked as three-dimensional tracks for biosensing. In the presence of a target, the nicking endonuclease cleavage process was triggered, leading to the generation of two strands (S1 and S2). Both S1 and S2 act as stimuli to participate in the strand displacement reaction on the [email protected]/MOFs, which caused the unlocking of the pore to release MB, resulting in the decrease of the signal. Using carcinoembryonic antigen (CEA) as a model target, the cascade-amplified biosensor presented good performance for CEA detection, ranging from 50 fg/mL to 10 ng/mL with a detection limit of 16 fg/mL. The stimuli-responsive DNA-gated MOF-based electrochemical platform exhibited three-dimensional biosensing tracks with rational utilization of the cascade amplification, providing an effective method for cancer biomarker detection.
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http://dx.doi.org/10.1021/acsami.9b18805DOI Listing
January 2020

Pharmacokinetics, Bioequivalence, and Safety Studies of Prucalopride in Healthy Chinese Subjects.

Clin Pharmacol Drug Dev 2020 08 3;9(6):671-676. Epub 2019 Dec 3.

Office of Drug Clinical Trial Institution, First Affiliated Hospital of Wenzhou Medical University, Zhejiang, China.

The objectives of the present study were to evaluate the bioequivalence of 2 tablet formulations of prucalopride, generic and branded, and to investigate relevant pharmacokinetic and safety profiles. This study was designed as a randomized, open-label, fasting, single-dose, crossover, and dual-period trial. After overnight fasting, 12 subjects were given prucalopride tablets via oral administration, and blood specimens were obtained up to 96 hours after dosing. Prucalopride concentrations in plasma were measured using ultraprecision liquid chromatography-tandem mass spectrometry followed by calculation of pharmacokinetic parameters. The safety of prucalopride was assessed throughout the study. The pharmacokinetics of prucalopride can be defined as a 2-compartment model with a long elimination phase. No significant differences were observed between the pharmacokinetic profiles of the generic and branded prucalopride tablets. Bioequivalence was evaluated using 90%CIs for the ratio test/reference of log area under the concentration-time curve over 96 hours, log area under the concentration-time curve to infinity, and log peak concentration from generic and branded tablets, which were 100.06-109.94%, 100.63-110.32%, and 95.84-113.08%, respectively. During administration of the medication, there were 18 adverse events in 6 subjects in the test formulation group and 19 cases of adverse events in 6 subjects in the reference formulation group (P > .05). No severe adverse effects were detected. These results suggest that generic and branded prucalopride tablets are bioequivalent and show similar safety profiles.
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http://dx.doi.org/10.1002/cpdd.754DOI Listing
August 2020

Fluorescent-Magnetic-Catalytic Nanospheres for Dual-Modality Detection of H9N2 Avian Influenza Virus.

ACS Appl Mater Interfaces 2019 Nov 28;11(44):41148-41156. Epub 2019 Oct 28.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering , Hubei University , Wuhan 430062 , PR China.

The outbreak of H9N2 avian influenza virus (H9N2 AIV) brings high mortality and huge economic losses every year. Sensitive and reliable detection methods are essential to timely diagnosis and treatment. Herein, a dual-modality immunoassay is proposed for H9N2 AIV detection by employing fluorescent-magnetic-catalytic nanospheres (FMCNs) as labels and alkaline phosphatase (ALP)-induced metallization as a signal amplification strategy. The excellent magnetic properties of FMCNs endow the assay a potential application in complex samples. Also, the excellent fluorescence properties of FMCNs enable fluorescence modality readout. The antibodies on the FMCN surface can achieve efficient capture and separation of targets. Amplified electrochemical modality readout can be obtained through ALP-catalyzed silver deposition. Dual-modality immunoassay combined the advantages of electrochemical assay with fluorescence assay and provides accurate detection results to meet different testing needs. With two quantitative analysis forms, H9N2 AIV can be detected by electrochemical signals with a quantitation range of 0.1 to 1000 ng/mL and a detection limit of 10 pg/mL. The linear range is 300 to 1000 ng/mL with a detection limit of 69.8 ng/mL by the fluorescence signal readout. Moreover, the specificity, anti-interference ability, accuracy, and diversity of the proposal have unlimited potential for early diagnosis of suspect infections.
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http://dx.doi.org/10.1021/acsami.9b16718DOI Listing
November 2019

Incidence and risk factors for epidural morphine induced pruritus in parturients receiving cesarean section: A prospective multicenter observational study.

Medicine (Baltimore) 2019 Oct;98(40):e17366

Department of Anesthesiology, Peking Union Medical College Hospital, Dongcheng District.

This study is designed to clarify the incidence of epidural morphine induced pruritus (EMIP) in Chinese Han and Tibetan parturients after cesarean section (CS) and to identify the correlated risk factors.This is a prospective multicenter observational study. Follow-up evaluations were performed at 3 hours, 6 hours, 12 hours, 24 hours, and 48 hours after morphine administration. The primary outcome was EMIP incidence. Other details were also recorded for risk factor screening.Totally 284 parturients receiving CS were enrolled and 247 completed the study. The overall incidence of EMIP was 18.6% (46 in 247). The onset of pruritus was 5.6 ± 4.8 hours (mean ± SD) after morphine administration and the duration of pruritus was 14.0 ± 8.8 hours. Logistic regression models was built with 5 variables, history of allergy, serotonin receptor antagonist administration, I.V. fentanyl administration, epidural morphine volume and VAS pain score. Results of showed that 2 of the variables, history of allergy (P < .001) and serotonin receptor antagonist (P < .05), were significantly correlated with incidence of EMIP.In conclusion, EMIP incidence in our study was 18.6%. Positive medical history of allergy and not using serotonin receptor antagonist were potential risk factors of EMIP development.Trial registration: ChiCTR-OPC-17012345.
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http://dx.doi.org/10.1097/MD.0000000000017366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783145PMC
October 2019

An exonuclease-assisted triple-amplified electrochemical aptasensor for mucin 1 detection based on strand displacement reaction and enzyme catalytic strategy.

Anal Chim Acta 2019 Dec 13;1086:75-81. Epub 2019 Aug 13.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China. Electronic address:

The development of some sensitive methods for MUC1 is critical for preclinical diagnosis of tumors. In this experiment, we built a triple-amplified electrochemical aptasensor to achieve sensitive detection of MUC1, which was based on exonuclease III (Exo III)-assisted with strand displacement reaction and enzyme catalytic strategy. Firstly, with the help of Exo III, MUC1 and aptamer could be recycled during the cycle I, the single stranded DNA-1 (S-1) was produced during the process and was introduced to the hybride reaction on the electrode. Secondly, during the cycle II, strand displacement reaction was triggered on the electrode with the adding of hairpin DNA-2 (H-2). Thirdly, after the gold nanoparticles (AuNPs)-DNA-enzyme conjugates hybrided with the H-2 on the electrode, the AuNPs-DNA-enzyme conjugates could act as signal probe to produce electrochemical catalytic signal. We used the fabricated triple-amplified electrochemical aptasensor that could detect MUC1 from 0.1 pg mL to 10 ng mL with the detection limit of 0.04 pg mL under the optimized experimental conditions. The constructed triple-amplified electrochemical aptasensor could be applied in real samples determination. Besides, the strategy can be applied to detect other proteins for health monitoring.
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http://dx.doi.org/10.1016/j.aca.2019.08.019DOI Listing
December 2019

Enrichment-Stowage-Cycle Strategy for Ultrasensitive Electrochemiluminescent Detection of HIV-DNA with Wide Dynamic Range.

Anal Chem 2019 10 12;91(19):12238-12245. Epub 2019 Sep 12.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules and College of Chemistry and Chemical Engineering , Hubei University , Wuhan 430062 , P. R. China.

Sensitive detection of human immunodeficiency virus DNA (HIV-DNA) is essential for timely diagnosis and cure of the illness. Herein, a novel "enrichment-stowage-cycle" strategy was proposed to fabricate a multiple amplified electrochemiluminecence (ECL) biosensor for HIV-DNA detection. On the basis of the enrichment role of magnetic nanobeads, assembly role of copolymer nanospheres and strand displacement amplification (SDA), the processes were named as "enrichment", "stowage", and "cycle", respectively. The method employed electrochemiluminescent nanospheres (ENs) as signal labels by assembling three layers of CdSe/ZnS quantum dots (QDs) onto the surface of copolymer nanospheres. Compared to QDs, the same concentration of ENs can the enhance the ECL intensity by about 11.3-fold. SDA could further amplify the signals by about 3.77-fold, possessing high sensitivity for low-abundant biomarkers detection. The integration of magnetic separation improved detection specificity and stability, making the method possible for practical application. On the basis of magnetic separation, ENs and SDA, the ECL biosensor realized ultrasensitive detection of 39.81 fM HIV-DNA, which was more sensitive than other HIV-DNA analytical methods, with a wide dynamic range of 0.05 pM to 50 nM. The successful detection of HIV-DNA in complex samples with good sensitivity and accuracy indicated its potential utilization in early judgment of diseases and fabrication of signal amplification platforms.
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http://dx.doi.org/10.1021/acs.analchem.9b01969DOI Listing
October 2019

Au-Luminol-decorated porous carbon nanospheres for the electrochemiluminescence biosensing of MUC1.

Nanoscale 2019 Sep;11(36):16860-16867

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministryof Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules&College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.

Electrochemiluminescence (ECL) nanomaterials are usually deposited compactly on the surface of electrodes, which may cause poor mass transfer of reactants, thereby resulting in low ECL efficiency. In this work, we developed a novel kind of luminescent material denoted as C-Au-luminol nanospheres (C-Au-Lum NSs) by high dispersion of luminophores on porous carbon nanospheres (PCNSs). C-Au-Lum NSs were facilely prepared by the in situ reduction of chloroauric acid with the luminescent reagent luminol (Lum) on the nano-pores of PCNSs. Plenty of luminescent Au-Lum NPs were dispersedly concentrated inside the numerous pores and hollow interiors of PCNSs, effectively increasing the mass transfer of reagents and accelerating the electron transport inside the porous nanospheres. This greatly improved the availability of luminophores and endowed C-Au-Lum NSs with excellent ECL emission. After further integrating with enzymatic circulation and strand displacement, an ultrasensitive ECL biosensor was achieved for the ultrasensitive detection of an important tumor biomarker, mucin1. The logarithmically linear range from 0.1 pg mL-1 to 1 ng mL-1 with the detection limit of 47.6 fg mL-1 (S/N = 3) was achieved, demonstrating the superior performance of C-Au-Lum NSs. This work would provide new ideas for the construction of high-performance ECL sensing platforms for diverse applications.
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http://dx.doi.org/10.1039/c9nr02190fDOI Listing
September 2019