Publications by authors named "Xiu Zhang"

177 Publications

Effects of palmitic acid and eicosapentaenoic acid on angiogenesis of porcine vascular endothelial cells.

Vet Med Sci 2021 Sep 21. Epub 2021 Sep 21.

Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou, China.

Restricted placental angiogenesis is an important cause of intrauterine growth retardation in piglets. During pregnancy, sow obesity can result in an increase in placental lipid deposition, subsequently inhibiting placental angiogenesis and fetal development. However, the effect of different types of fatty acids on placental angiogenesis is still unclear. Trophoblast cells and vascular endothelial cells constitute two important types of placental tissue. In this study, we used palmitic acid (C16:0) and eicosapentaenoic acid (C20:5, n-3), respectively, to treat porcine trophectoderm cells (pTr2) and porcine iliac artery endothelial cells (PIEC) to study the effects of saturated fatty acids and n-3 polyunsaturated fatty acids (PUFAs) on placental angiogenesis in vitro. We found that C16:0 caused significant cytotoxicity in pTr2 and PIEC (p < 0.01) and inhibited the proliferation and migration of PIEC (p < 0.01), whereas C20:5 treatment exhibited very low cytotoxicity and minimal inhibition of cellular proliferation. Meanwhile, a low concentration of C16:0 had no effect on the tube formation in PIEC, whereas C20:5 significantly promoted tube formation of PIEC (p < 0.01). These results suggested that saturated fatty acids and n-3 PUFAs had different effects on placental angiogenesis. As essential functional fatty acid, n-3 PUFA might be effective measure in alleviating the placental lipotoxicity caused by sow obesity during pregnancy.
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http://dx.doi.org/10.1002/vms3.577DOI Listing
September 2021

Comprehensive Analysis of circRNA Expression Profiles During Cervical Carcinogenesis.

Front Oncol 2021 31;11:676609. Epub 2021 Aug 31.

Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, China.

Circular RNAs (circRNAs) are regulatory molecules that participate in the occurrence, development and progression of tumors. To obtain a complete blueprint of cervical carcinogenesis, we analyzed the temporal transcriptomic landscapes of mRNAs and circRNAs. Microarrays were performed to identify the circRNA and mRNA expression profiles of cervical squamous cell carcinoma (CSCC) and high-grade squamous intraepithelial lesion (HSIL) patients compared with normal controls (NC). Short time-series expression miner (STEM) was utilized to characterize the time-course expression patterns of circRNAs and mRNAs from NC to HSIL and CSCC. A total of 3 circRNA profiles and 3 mRNA profiles with continuous upregulated patterns were identified and selected for further analysis. Furthermore, functional annotation showed that the mRNAs were associated with DNA repair and cell division. The protein-protein interaction (PPI) network analysis revealed that the ten highest-degree genes were considered to be hub genes. Subsequently, a competing endogenous RNA (ceRNA) network analysis and real-time PCR validation indicated that hsa_circ_0001955/hsa-miR-6719-3p/CDK1, hsa_circ_0001955/hsa-miR-1277-5p/NEDD4L and hsa_circ_0003954/hsa-miR-15a-3p/SYCP2 were highly correlated with cervical carcinogenesis. Silencing of hsa_circ_0003954 inhibited SiHa cell proliferation and perturb the cell cycle . This study provides insight into the molecular events regulating cervical carcinogenesis, identifies functional circRNAs in CSCC, and improves the understanding of the pathogenesis and molecular biomarkers of CSCC and HSIL.
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http://dx.doi.org/10.3389/fonc.2021.676609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438239PMC
August 2021

Re: Saxena et al.: Atropine for treatment of childhood myopia in India: multicentric randomized trial (Ophthalmology. 2021;128:1367-1369).

Ophthalmology 2021 Aug 24. Epub 2021 Aug 24.

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China. Electronic address:

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http://dx.doi.org/10.1016/j.ophtha.2021.08.003DOI Listing
August 2021

Elucidating the impacts of rapid urban expansion on air quality in the Yangtze River Delta, China.

Sci Total Environ 2021 Aug 3;799:149426. Epub 2021 Aug 3.

Key Lab of Aerosol Chemistry and Physics, SKLLQG, Institute of Earth Environment, Chinese Academy of Sciences, Xi'an, Shaanxi 710061, China.

Urban expansion not only results in land use transformation, but also introduces extra anthropogenic emissions over the expanded urban areas, which is usually neglected in existing studies. In this study, we consider both the changes in land use categories and added anthropogenic emissions from 2001 to 2018 in the Yangtze River Delta (YRD) which we define as the city of Shanghai and the nearby provinces of Zhejiang, Jiangsu, and Anhui, China and explore the individual and combined impacts of these factors on air pollution using the WRF-Chem model. Calibrated by available observations, the model performs well (IOA (index of agreement) > 0.8) in reproducing the meteorological fields and ambient PM and O concentrations in September 2018. We show that the land use transformation from non-urban to urban and the introduced anthropogenic emissions over new urban areas exert opposite influences on ambient PM concentrations over YRD, particularly in the expanded urban areas, and the PM decrease due to land use changes is significantly offset by the increase due to added emissions. The response of ambient O concentration to these two factors is highly variable in space, which is dependent on the chemical regime of tropospheric O formation and influenced by the chemistry-meteorology feedback. As the total effect, strong increases in O concentration occur over the central areas of YRD. These results highlight that it is essential to take into account the additional anthropogenic emissions over expanded urban areas in the assessment of environmental impacts of urban expansion.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149426DOI Listing
August 2021

Characterization of three glutamate decarboxylases from Bacillus spp. for efficient γ-aminobutyric acid production.

Microb Cell Fact 2021 Aug 4;20(1):153. Epub 2021 Aug 4.

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Background: Gamma-aminobutyric acid (GABA) is an important bio-product used in pharmaceuticals and functional foods and as a precursor of the biodegradable plastic polyamide 4. Glutamate decarboxylase (GAD) converts L-glutamate (L-Glu) into GABA via decarboxylation. Compared with other methods, develop a bioconversion platform to produce GABA is of considerable interest for industrial use.

Results: Three GAD genes were identified from three Bacillus strains and heterologously expressed in Escherichia coli BL21 (DE3). The optimal reaction temperature and pH values for three enzymes were 40 °C and 5.0, respectively. Of the GADs, GADZ11 had the highest catalytic efficiency towards L-Glu (2.19 mM s). The engineered E. coli strain that expressed GADZ11 was used as a whole-cell biocatalyst for the production of GABA. After repeated use 14 times, the cells produced GABA with an average molar conversion rate of 98.6% within 14 h.

Conclusions: Three recombinant GADs from Bacillus strains have been conducted functional identification. The engineered E. coli strain heterologous expressing GADZ1, GADZ11, and GADZ20 could accomplish the biosynthesis of L-Glu to GABA in a buffer-free reaction at a high L-Glu concentration. The novel engineered E. coli strain has the potential to be a cost-effective biotransformation platform for the industrial production of GABA.
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http://dx.doi.org/10.1186/s12934-021-01646-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336373PMC
August 2021

Validating the Experiences in Close Relationships-Relationship Structures Scale among Chinese Children and Adolescents.

J Pers Assess 2021 Jul 22:1-12. Epub 2021 Jul 22.

Faculty of Psychology, Beijing Normal University, Beijing, China.

The Experiences in Close Relationships-Relationship Structures (ECR-RS) scale is designed to capture attachment among diverse relational contexts (e.g., parents, friends). Although the ECR-RS has begun to be applied to children and adolescents, its psychometric properties among children and adolescents are not well-known, especially concerning second-order structural validity, measurement invariance, and longitudinal predictive validity. To fill this gap, the current research examined the ECR-RS among 3,184 Chinese students (9- to 18-year old) using cross-sectional and longitudinal (6 months) data. The results demonstrated generally acceptable composite and test-retest reliabilities, and acceptable second-order structural validity. In addition, the measurement invariance of the ECR-RS held across time, relational contexts, and school levels to varying degrees. Furthermore, using a partial invariance model, we depicted the cross-sectional trajectory of attachment scores across relational contexts and school levels. Finally, attachment was associated concurrently with the Big-Five personality traits in theoretically meaningful ways and longitudinally predicted depression, anxiety, stress, and self-esteem after controlling for age, gender, and pretest scores. In conclusion, the Chinese ECR-RS has proven to be a valuable instrument for future research among children and adolescents.
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http://dx.doi.org/10.1080/00223891.2021.1947844DOI Listing
July 2021

Development and validation of a clinical prediction model for endocervical curettage decision-making in cervical lesions.

BMC Cancer 2021 Jul 13;21(1):804. Epub 2021 Jul 13.

Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, China.

Background: In the absence of practical and reliable predictors for whether the endocervical curettage (ECC) procedure should be performed, decisions regarding patient selection are usually based on the colposcopists' clinical judgment instead of evidence. We aimed to develop and validate a practical prediction model that uses available information to reliably estimate the need to perform ECC in patients suspected of having cervical lesions.

Methods: In this retrospective study, 2088 patients who underwent colposcopy, colposcopically directed biopsy (CDB) and ECC procedures between September 2019 and September 2020 at the Second Hospital of Shanxi Medical University were included. The data were analyzed with univariate and multivariable logistic regression. Least absolute shrinkage and selection operator (LASSO) was used to select predictors for ECC positivity. The ECC prediction model was presented as a nomogram and evaluated in terms of discrimination and calibration. Furthermore, this model was validated internally with cross-validation and bootstrapping.

Results: Significant trends were found for ECC positivity with increasing age (P = 0.001), menopause (P = 0.003), Human papillomavirus (HPV) status (P < 0.001), severity of ThinPrep Cytological Test (TCT) (P < 0.001), original squamous epithelium ectopia (P = 0.037) and colposcopy impression (P < 0.001) by multivariable logistic regression analysis. The ECC prediction model was developed based on the following predictors: age, menopause, symptom of contact bleeding, severity of TCT, HPV status, cervix visibility, original squamous epithelium ectopia, acetowhite changes and colposcopic impression. This model had satisfactory calibration and good discrimination, with an area under the receiver operator characteristic curve (AUC) of 0.869 (95% confidence interval 0.849 to 0.889).

Conclusions: A readily applicable clinical prediction model was constructed to reliably estimate the probability of ECC positivity in patients suspicious of having cervical lesions, which may help clinicians make decisions regarding the ECC procedure and possibly prevent adverse effects.
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http://dx.doi.org/10.1186/s12885-021-08523-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276473PMC
July 2021

Prevalence of strabismus and its risk factors among school aged children: The Hong Kong Children Eye Study.

Sci Rep 2021 Jul 5;11(1):13820. Epub 2021 Jul 5.

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, 4/F, Hong Kong Eye Hospital, 147K Argyle Street, Kowloon, Hong Kong SAR, China.

The study aims to determine the prevalence of strabismus and its risk factors among school children in Hong Kong. This is a cross-sectional study involving 6-8 year old children from different districts in Hong Kong. 4273 children received comprehensive ophthalmological examination, cycloplegic auto-refraction, best corrected visual acuity (BCVA), anterior segment examination, cover/uncover test, ocular motility, and fundus examination. Demographic information, pre- and post- natal background, parental smoking status, and family history of strabismus were obtained through questionnaires. Strabismus was found among 133 children (3.11%, 95% CI 2.59-3.63%), including 117 (2.74%) exotropia and 12 (0.28%) esotropia cases (exotropia-esotropia ratio: 9.75:1). There was no significant difference in prevalence across age (6-8 years) and gender. Multivariate analysis revealed associations of strabismus with myopia (≤ - 1.00D; OR 1.61; 95% CI 1.03-2.52; P = 0.037) hyperopia (≥ + 2.00D; OR 2.49; 95% CI 1.42-4.39; P = 0.002), astigmatism (≥ + 2.00D; OR 2.32; 95% CI 1.36-3.94; P = 0.002), and anisometropia (≥ 2.00D; OR 3.21; 95% CI 1.36-7.55; P = 0.008). Other risk factors for strabismus included maternal smoking during pregnancy (OR 4.21; 95% CI 1.80-9.81; P = 0.001), family history of strabismus (OR 6.36; 95% CI 2.78-14.50, P < 0.0001) and advanced maternal age at childbirth (> 35 years; OR 1.65; CI 1.09-2.49, P = 0.018). The prevalence of strabismus among children aged 6-8 years in Hong Kong is 3.11%. Refractive errors, family history of strabismus and maternal smoking history during pregnancy are risk factors. Early correction of refractive errors and avoidance of maternal smoking during pregnancy are potentially helpful in preventing strabismus.
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http://dx.doi.org/10.1038/s41598-021-93131-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257606PMC
July 2021

Reply.

Ophthalmology 2021 Oct 17;128(10):e72. Epub 2021 Jun 17.

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China. Electronic address:

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http://dx.doi.org/10.1016/j.ophtha.2021.05.025DOI Listing
October 2021

Optimized seeding rate and nitrogen topdressing ratio for simultaneous improvement of grain yield and bread-making quality in bread wheat sown on different dates.

J Sci Food Agric 2021 Jun 18. Epub 2021 Jun 18.

National Key Lab. of Crop Biology, Key Lab. of Crop Ecophysiology and Farming System, Ministry of Agriculture, Agronomy College of Shandong Agricultural University, Tai'an, P.R. China.

Background: Sowing date, seeding rate, and nitrogen (N) topdressing ratio have strong effects on grain yield (GY) and bread-making quality (BQ) in bread wheat. Simultaneous improvement in GY and BQ in bread wheat has long been a challenge due to the inverse relationship between GY and grain protein concentration (GPC). In this study, we investigated whether the GY and BQ of bread wheat sown on different dates could be improved simultaneously by optimizing the seeding rate and the N topdressing ratio.

Results: Delaying sowing beyond a certain period led to decreases in both GY and BQ. Optimizing the seeding rate and N topdressing ratio enhanced the N uptake during pre- and post-anthesis, as well as N remobilization during grain filling for all wheat plants sown on different dates, thereby increasing the GPC and the total N per grain (N ). Consequently, grain protein composition was improved, resulting in an increased glutenin/gliadin ratio, sodium dodecyl sulfate-insoluble glutenin/total glutenin (i.e., glutenin polymerization index), and high-molecular-weight glutenin subunit/ low-molecular-weight glutenin subunit (HMW-GS/LMW-GS) ratio. Increased GPC and improved grain protein composition enhanced BQ.

Conclusion: The mechanism underlying simultaneous improvement in GY and GPC as well as N was the greater increase in N accumulation in grains per unit area relative to increases in GY, or total grain number per unit area. The GY and BQ can be improved simultaneously regardless of sowing date by optimizing the seeding rate and N topdressing ratio via enhanced N uptake and N remobilization into grains. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11366DOI Listing
June 2021

[Spatiotemporal Distribution of Aerosol Optical Depth Based on Landsat Data in the Hinterland of the Guanzhong Basin and Its Relationship with Urbanization].

Huan Jing Ke Xue 2021 Jun;42(6):2699-2712

College of Urban and Environmental Science, Northwest University, Xi'an 710127, China.

Aerosol optical depth (AOD) is one of the most fundamental optical properties of aerosols that characterizes the attenuation of light by aerosols and is an indicator of regional air pollution. Based on the blue band surface reflectance database from the MOD09A1 products for the period 2000-2019 and the ASTER spectral database, AOD was estimated from Landsat TM/OLI data using the deep blue algorithm (DB). Multi-year average/annual average and seasonal AOD values for the period 2000-2019 were then calculated to analyze the spatial characteristics and temporal variations of AOD using the empirical orthogonal function method (EOF). Furthermore, the impacts of urbanization on the spatio-temporal distribution of AOD were analyzed. The obtained results are summarized as follows:① The multi-year average AOD spatial distribution in the hinterland of the Guanzhong Basin was significantly affected by topography and human activities, with higher AOD values and variationsin areas of low altitude and high-intensity human activities compared to the surrounding mountains. Thus, changes in AOD in the study area are mainly affected by anthropogenic factors. AOD also showed significant seasonal variations, whereby spring (0.34) > summer (0.33) > autumn (0.23) > winter (0.12), and the largest regional differences occurred in summer; ② The annual average AOD (from 2000-2019) showed the trend of "increase-decrease-increase", and reached a maximum in 2005, with the high AOD area gradually moving to the south. The distribution of AOD values in spring and summer was relatively discrete, while it is in a low-value agglomeration state in winter; ③ Three main AOD spatial distribution modes were identified based on the EOF, which had cumulative contribution rate of 83.0%. The spatial distribution trend of AOD showed regional consistency, with feature vectors consistent with the altitude, thus reflecting the difference of AOD at different altitudes. Taking the Qinling Mountains as the dividing line, the AOD presented the "north-south" pattern, AOD showed a "north-south" pattern, reflecting the uniqueness of the regional development in the Guanzhong Basin compared to the southern Qinling Mountains. The "southeast-northwest" distribution pattern indicated that the AOD presented a reverse change trend between urban and non-urban; and ④ The results of correlation analysis between the AOD and urbanization revealed a positive correlation with permanent population density (=0.707, <0.05), impervious surface density (=0.377, <0.05), and industrial POI density (=0.727, <0.5). These results are significant for improving the monitoring of air quality in the Guanzhong Basin and for the construction of an urban ecological environment.
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http://dx.doi.org/10.13227/j.hjkx.202010018DOI Listing
June 2021

The association between attention-deficit/hyperactivity disorder and retinal nerve fiber/ganglion cell layer thickness measured by optical coherence tomography: a systematic review and meta-analysis.

Int Ophthalmol 2021 Sep 20;41(9):3211-3221. Epub 2021 May 20.

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Sha Tin, Hong Kong.

Purpose: Retinal nerve fiber/ganglion cell layer (RNFL/GCL) thickness measured using optical coherence tomography has been proposed as an ocular biomarker for children with attention-deficit/hyperactivity disorder (ADHD), but findings varied in different studies. This study aims to determine the association between RNFL/GCL thickness and ADHD in children by systematic review and meta-analysis.

Methods: We performed a literature search in Embase, PubMed, Medline, Web of Science, and PsycINFO for relevant articles published up to February 29, 2020. All studies with original data comparing RNFL/GCL thickness in ADHD and healthy children were included. The Newcastle Ottawa Scale was used to assess bias risk and quality of evidence. Pooled estimates of the differences in thickness of RNFL or GCL between ADHD and healthy subjects were generated using meta-analysis with a random-effect model due to significant inter-study heterogeneity. Sensitivity analysis was also performed.

Results: We identified four eligible studies involving a total of 164 ADHD and 150 control subjects. Meta-analysis revealed that ADHD in children was associated with a reduction in global RNFL thickness (SMD, - 0.23; 95% CI - 0.46, - 0.01; p = 0.04). The global GCL thickness was examined in two studies with 89 ADHD and 75 control subjects, but the pooled difference in global GCL thickness between ADHD children and controls was not statistically significant (SMD, - 0.34; 95% CI - 1.25, 0.58; p = 0.47).

Conclusion: Existing evidence suggests a possible association between ADHD and RNFL thinning in children. In view of the limited number of reports, further studies in large cohorts should be warranted.
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http://dx.doi.org/10.1007/s10792-021-01852-8DOI Listing
September 2021

A Sensitive LC-MS/MS Method for the Determination of Afatinib in Human Plasma and Its Application to a Bioequivalence Study.

J Chromatogr Sci 2021 Apr 29. Epub 2021 Apr 29.

BE/Phase I clinical center, The first affiliated hospital of Xiamen university, Xiamen, Fujian 361000, China.

A high performance liquid chromatography-tandem mass spectrometry assay for the determination of afatinib (AFT) in human plasma was established. A simple sample preparation of protein precipitation was used and separation was achieved on a C18 column by the gradient mixture of mobile Phase A of water (containing 0.1% ammonia) and the mobile Phase B of acetonitrile and water (V:V = 95:5, containing 0.2% ammonia). The multiple reaction monitoring mode was used to monitor the precursor-to-production transitions of m/z 486.2 → m/z 371.4 for AFT and m/z 492.2 → m/z 371.3 for AFT-d6 (internal standard) at positive ionization mode. The calibration curve ranged from 0.100 to 25.0 ng·mL-1 and the correlation coefficient was greater than 0.99. The intra- and inter-batch precision was less than or equal to 10.0%. Accuracy determined at four concentrations was in the range of 92.3-103.3%. In summary, our method was sensitive, simple and reliable for the quantification of AFT and was successfully applied to a bioequivalence study.
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http://dx.doi.org/10.1093/chromsci/bmab040DOI Listing
April 2021

The Emergent Pelvic Artery Embolization in the Management of Postpartum Hemorrhage: A Systematic Review and Meta-analysis.

Obstet Gynecol Surv 2021 Apr;76(4):234-244

Assistant Professor, Department of Obstetrics and Gynecology, Shunde Hospital of Southern Medical University (The First People's Hospital of Shunde), Foshan, Guangdong, China.

Importance: Postpartum hemorrhage (PPH) is an emergent obstetric complication and the leading cause of maternal mortality. Pelvic arterial embolization (PAE) is an effective treatment for intractable PPH. However, a unique protocol has not been accepted in obstetrical practice.

Objective: To evaluate its efficiency, safety, complications, and outcomes, we conducted a systematic review and meta-analysis of PAE for PPH in the literature.

Evidence Acquisition: The Medline, the database of abstract of reviews, the index to allied health literature, and the Chinese database Sino-Med were searched on March 31, 2020, for studies on PAE for PPH. The data for PAE indication, agents, arteries, success rate, complications, and outcomes were extracted and syncretized for meta-analysis.

Results: From 1075 identified articles, 113 abstracts or full articles were retrieved and 43 studies were finally identified as meeting the including criteria. The results demonstrated that the indications for PAE were as follows: uterine atony, placental abnormality, delivery tract injury, disseminated intravascular coagulation, arteriovenous malformation, and vaginal hematoma. The embolization agents mostly in order were gelatin sponge particles, polyvinyl alcohol particles, Gelfoam, -butyl cyanoacrylate, microcoil, and glue; for arteries, they were mostly uterine artery and internal iliac artery. The clinical success rate was 90.5%, whereas the technical success rate was 99.3%. The most common complications of PAE were postembolization syndrome and menstrual abnormality.

Conclusions And Relevance: The emergent PAE is a safe and effective method with high success rate in life-threatening PPH management. Gelatin sponge granules measuring 500 to 1000 μm in diameter have safe results. Pelvic arterial embolization may affect the recovery of menses and increase PPH in the subsequent pregnancy, but there was no noted correlation with fetal growth restriction.
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http://dx.doi.org/10.1097/OGX.0000000000000887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081441PMC
April 2021

[Effects of potassium-solubilizing bacteria promoting the growth of seedlings under salt stress.]

Ying Yong Sheng Tai Xue Bao 2021 Apr;32(4):1289-1297

College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China.

We investigated the effects of potassium-releasing bacteria on physiological and bioche-mical characteristics of (Cultivar Ningqi 1) under salt stress, with an experiment with treatments following randomized block design. The treatments included control (CK), 100 mmol·L NaCl stress (NaCl), 100 mmol·LNaCl stress+KSBGY01 bacteria (NaCl-M1), 100 mmol·LNaCl stress+KSBGY02 bacteria (NaCl-M2), and 100 mmol·LNaCl stress+KSBGY01+KSBGY02 (NaCl-M3). We measued chlorophyll content, polyphenol content, superoxide anion (O) content, hydrogen peroxide (HO) content, soluble sugar content, antioxidant enzyme activity and sucrose metabolic enzyme activity of seedlings. Results showed that the presence of potassium bacteria increased the values of flavonoids (FLAV), fluorescence excitation than anthocyanins relative index (FERARI), anthocyanins (ANTH-RB), nitrogen balance index (NBI-G), decreased the contents of O and HO, and improved soluble sugar content, catalase (CAT) activity, sucrose phosphate synthase (SPS) activity, sucrose synthase (SS) activity and invertase (INV) activity of leaves in seedlings under salt stress. Among all the treatments, the highest values of ANTH-RB and NBI-G, soluble sugar content, and activities of CAT, SPS, SS, and INV presented in NaCl-M2 treatment, the highest values of SPAD, FLAV, and FERARI presented in NaCl-M3 treatment, the highest activity of superoxide dismutase (SOD) presented in NaCl-M1 treatment, the highest activity of glutathione peroxidase (GSH-Px) presented in NaCl treatment, and the highest peroxisome (POD) activity presented in CK. The 14 significant physiological and biochemical indicators in the leaves of seedling were analyzed by grey system correlation degree method. Our results suggested that the weighted correlation degree of phy-siological and biochemical indices of inoculated potassium-solubilizing bacteria was higher than that under CK and NaCl treatments. The highest weighted correlation was observed in NaCl-M2 treatment. Therefore, adding KSBGY02 potassium-solubilizing bacteria could alleviate the salt stress for seedlings.
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http://dx.doi.org/10.13287/j.1001-9332.202104.021DOI Listing
April 2021

Differences in microbiota between acute and chronic perianal eczema.

Medicine (Baltimore) 2021 Apr;100(16):e25623

Department of Internal Medicine, Beijing Coloproctological Hospital, Beijing Erlonglu Hospital, Beijing, China.

Abstract: Microbiota has been suggested to play a role in patients with intestinal and cutaneous diseases. However, the profiling of perianal eczema microbiota has not been described. We have explored the general profile and possible differences between acute and chronic perianal eczema. A total of 101 acute perianal eczema (APE) and 156 chronic perianal eczema (CPE) patients were enrolled in this study and the perianal microbiota was profiled via Illumina sequencing of the 16S rRNA V4 region.The microbial α-diversity and structure are similar in APE and CPE patients; however, the perianal microbiota of the APE patients had a higher content of Staphylococcus (22.2%, P < .01) than that of CPE patients. Top10 genera accounting for more than 60% (68.81% for APE and 65.47% for CPE) of the whole microbiota, including Prevotella, Streptococcus, and Bifidobacterium, show an upregulation trend in the case of APE without reaching statistically significant differences. This study compared the microbiota profiles of acute and chronic perianal eczema. Our results suggest that the microbiota of acute perianal eczema patients is enriched in Staphylococcus compared with that in the chronic group. Our findings provide data for further studies.
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http://dx.doi.org/10.1097/MD.0000000000025623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078401PMC
April 2021

Association of polymorphisms in , and with myopia progression and polygenic risk prediction in children.

Br J Ophthalmol 2021 Apr 2. Epub 2021 Apr 2.

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China

Aims: To assess the association of single-nucleotide polymorphisms (SNPs) with myopia progression for polygenic risk prediction in children.

Methods: Six SNPs ( rs4373767, rs13382811, rs7744813, rs2073560, rs7839488 and rs524952) were analysed in 1043 school children, who completed 3-year follow-up, using TaqMan genotyping assays. SNP associations with progression in spherical equivalent (SE) were analysed by logistic regression. Polygenic risk scores (PRS) were applied for computing the sum of the risk alleles of multiple SNPs corresponding to myopia progression, weighted by the effect sizes of corresponding SNPs.

Results: rs524952 showed significant association with fast progression (OR=1.32, 95% CI 1.10 to 1.59; p=0.003) and rs7744813 had nominal association (OR=1.32, 95% CI 1.04 to 1.67; p=0.02). In quantitative traits locus analysis, rs524952 and rs7744813 were associated with progression in SE (β=-0.038 D/year, p=0.008 and β=-0.042 D/year, p=0.02) and axial elongation (β=0.016 mm/year, p=0.01 and β=0.017 mm/year, p=0.027). rs13382811 also showed nominal association with faster progression in SE (β=-0.041 D/year, p=0.02). PRS analysis showed that children with the highest PRS defined by rs13382811, rs7744813 and rs524952 had a 2.26-fold of increased risk of fast myopia progression (p=4.61×10). PRS was also significantly associated with SE progression (R=1.6%, p=3.15×10) and axial elongation (R=1.2%, p=2.6×10).

Conclusions: In this study, multi-tiered evidence suggested SNPs in , and as risk factors for myopia progression in children. Additional attention and appropriate interventions should be given for myopic children with high-risk PRS as defined by rs524952, rs7744813 and rs13382811.
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http://dx.doi.org/10.1136/bjophthalmol-2020-318708DOI Listing
April 2021

A Molecularly Imprinted Polymer Sensor Based on the Electropolymerization of -Aminothiophenol-Functionalized Au Nanoparticles Electrode for the Detection of Nonylphenol.

Biomed Environ Sci 2020 Nov;33(11):887-891

Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, Wuhan 430065, P. R. China.

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http://dx.doi.org/10.3967/bes2020.122DOI Listing
November 2020

Combined effects of 3-nitrooxypropanol and canola oil supplementation on methane emissions, rumen fermentation and biohydrogenation, and total tract digestibility in beef cattle.

J Anim Sci 2021 Apr;99(4)

Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta T1J 4B1, Canada.

The individual and combined effects of 3-nitrooxypropanol (3-NOP) and canola oil (OIL) supplementation on enteric methane (CH4) and hydrogen (H2) emissions, rumen fermentation and biohydrogenation, and total tract nutrient digestibility were investigated in beef cattle. Eight beef heifers (mean body weight ± SD, 732 ± 43 kg) with ruminal fistulas were used in a replicated 4 × 4 Latin square with a 2 (with and without 3-NOP) × 2 (with and without OIL) arrangement of treatments and 28-d periods (13 d adaption and 15 d measurements). The four treatments were: control (no 3-NOP, no OIL), 3-NOP (200 mg/kg dry matter [DM]), OIL (50 g/kg DM), and 3-NOP (200 mg/kg DM) plus OIL (50 g/kg DM). Animals were fed restrictively (7.6 kg DM/d) a basal diet of 900 g/kg DM barley silage and 100 g/kg DM supplement. 3-NOP and OIL decreased (P < 0.01) CH4 yield (g/kg DM intake) by 31.6% and 27.4%, respectively, with no 3-NOP × OIL interaction (P = 0.85). Feeding 3-NOP plus OIL decreased CH4 yield by 51% compared with control. There was a 3-NOP × OIL interaction (P = 0.02) for H2 yield (g/kg DM intake); the increase in H2 yield (P < 0.01) due to 3-NOP was less when it was combined with OIL. There were 3-NOP × OIL interactions for molar percentages of acetate and propionate (P < 0.01); individually, 3-NOP and OIL decreased acetate and increased propionate percentages with no further effect when supplemented together. 3-NOP slightly increased crude protein (P = 0.02) and starch (P = 0.01) digestibilities, while OIL decreased the digestibilities of DM (P < 0.01) and neutral detergent fiber (P < 0.01) with no interactions (P = 0.15 and 0.10, respectively). 3-NOP and OIL increased (P = 0.04 and P < 0.01, respectively) saturated fatty acid concentration in rumen fluid, with no interaction effect. Interactions for ruminal trans-monounsaturated fatty acids (t-MUFA) concentration and percentage were observed (P = 0.02 and P < 0.01); 3-NOP had no effect on t-MUFA concentration and percentage, while OIL increased the concentration (P < 0.01) and percentage (P < 0.01) of t-MUFA but to a lesser extent when combined with 3-NOP. In conclusion, the CH4-mitigating effects of 3-NOP and OIL were independent and incremental. Supplementing ruminant diets with a combination of 3-NOP and OIL may help mitigate CH4 emissions, but the decrease in total tract digestibility due to OIL may decrease animal performance and needs further investigation.
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http://dx.doi.org/10.1093/jas/skab081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051842PMC
April 2021

Effects of robot-assisted training on balance function in patients with stroke: A systematic review and meta-analysis.

J Rehabil Med 2021 Apr 1;53(4):jrm00174. Epub 2021 Apr 1.

Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Objective: To investigate the effectiveness of robot-assisted therapy on balance function in stroke survivors.

Data Sources: PubMed, the Cochrane Library, Embase and China National Knowledge Infrastructure databases were searched systematically for relevant studies.

Study Selection: Randomized controlled trials reporting robot-assisted therapy on balance function in patients after stroke were included.

Data Extraction: Information on study characteristics, demographics, interventions strategies and outcome measures were extracted by 2 reviewers.

Data Synthesis: A total of 19 randomized trials fulfilled the inclusion criteria and 13 out of 19 were included in the meta-analysis. Analysis revealed that robot-assisted therapy significantly improved balance function assessed by berg balance scale (weighted mean difference (WMD) 3.58, 95% confidence interval (95% CI) 1.89-5.28, p < 0.001) compared with conventional therapy. Secondary analysis indicated that there was a significant difference in balance recovery between the conventional therapy and robot-assisted therapy groups in the acute/subacute stages of stroke (WMD 5.40, 95% CI 3.94-6.86, p < 0.001), while it was not significant in the chronic stages. With exoskeleton devices, the balance recovery in robot-assisted therapy groups was significantly better than in the conventional therapy groups (WMD 3.73, 95% CI 1.83-5.63, p < 0.001). Analysis further revealed that a total training time of more than 10 h can significantly improve balance function (WMD 4.53, 95% CI 2.31-6.75, p < 0.001). No publication bias or small study effects were observed according to the Cochrane Collaboration tool.

Conclusion: These results suggest that robot-assisted therapy is an effective intervention for improving balance function in stroke survivors.
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http://dx.doi.org/10.2340/16501977-2815DOI Listing
April 2021

Simultaneous fluorescence determination of bisphenol A and its halogenated analogs based on a molecularly imprinted paper-based analytical device and a segment detection strategy.

Biosens Bioelectron 2021 May 20;180:113106. Epub 2021 Feb 20.

State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei, 430030, China. Electronic address:

Bisphenol A (BPA) and its halogenated analogs tetrabromobisphenol A (TBBPA) and tetrachlorobisphenol A (TCBPA) are common environmental contaminants and a method for their simultaneous determination is urgently needed. A paper-based analytical device (PAD) was prepared using a metal-organic framework of UiO-66-NH coated with molecularly imprinted polymers (MIPs) using TBBPA as a template. The maximum adsorption capacity was 120.94 mg g and the imprinting factor was 4.07. The selective recognition ability of this PAD enabled the effective separation of TBBPA, TCBPA and BPA based on paper chromatography. Subsequently, the PAD cut into segments were used individually to determine the presence of target chemicals using a highly sensitive fluorescent method. Under ultraviolet light irradiation, UiO-66-NH acts as a photocatalyst to produce reactive oxygen species (ROS) that degrade TBBPA, TCBPA or BPA in the imprinted cavities and the fluorescent signal of 2',7'-dichlorodihydrofluorescein diacetate (HDCFDA) added as a ROS probe enabled the indirect determination of target chemicals. This method could determine BPA and its halogenated analogs in dust samples simultaneously with detection limits ranging from 0.14 to 0.30 ng g. The intraday relative standard deviation (RSD) was ≤6.8% and interday RSD was ≤8.1%. The recoveries ranged from 91.0 to 105.6% with RSD values that were ≤7.5%. The results stemmed from this method were consistent with those obtained from LC-MS/MS. It is an environmentally-friendly approach due to the degradation of target pollutants and possesses many advantages such as high selectivity, low cost and easy-to-fabrication.
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http://dx.doi.org/10.1016/j.bios.2021.113106DOI Listing
May 2021

UPLC-MS/MS for the Simultaneous Determination of Sildenafil and N-Desmethyl Sildenafil.

J Chromatogr Sci 2021 Feb 1. Epub 2021 Feb 1.

BE/Phase I Clinical Center, The First Affiliated Hospital of Xiamen University, No. 55, Zhenhai Road, Xiamen, China.

A sensitive high-performance liquid chromatography-tandem mass spectrometry method was established for the simultaneous determination of sildenafil and N-desmethyl sildenafil in human plasma. The protein precipitation was used for extraction and the gradient elution of the mobile phase A of water (containing 0.01% formic acid) and the mobile phase B of acetonitrile, and methanol (V:V = 1:1, containing 0.01% formic acid) was used for chromatographic separation on a C18 column. Quantification was performed by multiple reaction monitoring mode to monitor the precursor-to-product ion transitions of m/z 475.4 → m/z 283.3 for sildenafil, m/z 461.4 → m/z 283.2 for N-desmethyl sildenafil, m/z 483.3 → m/z 108.1 for sildenafil-d8 (IS) and m/z 469.2 → m/z 283.3 for N-desmethyl sildenafil-d8 (IS) at the positive ionization mode. The intra- and inter-day relative standard deviations were less than 6.8% and 4.1% for sildenafil and N-desmethyl sildenafil, respectively. Accuracy at four levels ranged from 93.1% to 115.9% for sildenafil and 95.6% to 112.5% for N-desmethyl sildenafil. The present method was sensitive and reliable for simultaneous quantification of sildenafil and its active metabolite and was successfully applied to a pharmacokinetic study of an oral low dose of sildenafil in Chinese healthy volunteers.
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http://dx.doi.org/10.1093/chromsci/bmaa138DOI Listing
February 2021

Time Window for Ischemic Stroke First Mobilization Effectiveness: Protocol for an Investigator-Initiated Prospective Multicenter Randomized 3-Arm Clinical Trial.

Phys Ther 2021 05;101(5)

Institute for Disaster Management and Reconstruction of Sichuan University, Chengdu, China.

Objective: The purpose of this study is to investigate the optimal time window for initiating mobilizing after acute ischemic stroke.

Methods: The TIME Trial is a pragmatic, investigator-initiated, multi-center, randomized, 3-arm parallel group, clinical trial. This trial will be conducted in 57 general hospitals in mainland China affiliated with the China Stroke Databank Center and will enroll 6033 eligible patients with acute ischemic stroke. Participants will be randomly allocated to either (1) the very early mobilization group in which mobilization is initiated within 24 hours from stroke onset, (2) the early mobilization group in which mobilization begins between 24 and 72 hours poststroke, or (3) the late mobilization group in which mobilization is started after 72 hours poststroke. The mobilization protocol is otherwise standardized and identical for each comparison group. Mobilization is titrated by baseline mobility level and progress of patients throughout the intervention period. The primary outcome is death or disability assessed with the modified Rankin scale at 3 months poststroke. Secondary outcomes include impairment score of the National Institutes of Health Stroke Scale, dependence in activities of daily living as measured using the modified Barthel Index, cognitive ability assessed with the Mini-Mental State Examination, incidence of adverse events, hospital length of stay, and total medical costs.

Impact: The TIME Trial is designed to answer the question "when is the best time to start mobilization after stroke?" The effect of timing is isolated from the effect of type and dose of mobilization by otherwise applying a standard mobilization protocol across groups. The TIME Trial may, therefore, contribute to increasing the knowledge base regarding the optimal time window for initiating mobilization after acute ischemic stroke.
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http://dx.doi.org/10.1093/ptj/pzab038DOI Listing
May 2021

[Interactive effects of irrigation regime and planting density on grain yield and water use efficiency in winter wheat].

Ying Yong Sheng Tai Xue Bao 2021 Jan;32(1):163-174

College of Agronomy, Shandong Agricultural University, Tai'an 271018, Shandong, China.

To get an optimal irrigation regime and planting density for simultaneous improvement of grain yield (GY) and water use efficiency (WUE) in winter wheat, we examined the responses of 'Tainong 18' (with bigger ears) and 'Shannong 22' (with medium-sized ears) under four irrigation regimes, including 0, 45, 60, and 75 mm. Those two cultivars were planted at four densities: Tainong 18 at 135×10, 270×10, 405×10, and 540×10 plants·hm and Shannong 22 at 90×10, 180×10, 270×10, and 360×10 plants·hm. The interactive effects of irrigation regimes and plant densities on GY, water consumption characteristics, and WUE were investigated. The results showed that GY, evapotranspiration, soil water consumption, and WUE were significantly affected by irrigation regime, plant density, and their interaction. The optimal irrigation regime was 45 mm for both cultivars, while the optimal plant density was 405×10 plants·hm for Tainong 18 and 270×10 plants·hm for Shannong 22, as indicated by the highest GY, the lowest ratio of soil evaporation to evapotranspiration after jointing, and higher WUE and the ratio of soil water consumption below 1 m to total soil water consumption. The rational combination of plant density and irrigation could reduce unnecessary water consumption and improve WUE.
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http://dx.doi.org/10.13287/j.1001-9332.202101.025DOI Listing
January 2021

Community-Based Family Workshop Intervention Improved the Social Adaptation of Left-Behind Children in Rural China.

Front Public Health 2020 11;8:506191. Epub 2020 Dec 11.

Department of Maternal and Child Health and Sun Yat-sen Global Health Institute, School of Public Health and Institute of State Governance, Sun Yat-sen University, Guangzhou, China.

With the rapidly developing economy in China, there are more than 50 million left-behind children (LBC) in rural China, whose social adaptation has become a public concern. Thus, the current study aimed to investigate the effect of community-based family workshop intervention on social adaptation among rural LBC in China. A cluster randomized trial was conducted with 104 LBC, in which 66 LBC-caregivers dyads received guidance from community-based family workshop for 6 months, while the controls (LBC = 38) received routinely parenting guidance. Social adaptation of the children was assessed by the Strength and Difficulties Questionnaire (SDQ) at the beginning and the end of the intervention. Compared with controls, results showed remarkable improvement on emotional symptoms ( = 0.050), peer problems ( = 0.050), and total difficulties score (TDS, = 0.040) in the intervention group, especially those aged 3-6 years. Moreover, SDQ score of TDS ( = 0.039), peer problems ( = 0.013), and hyperactivity-inattention ( = 0.023) decreased after intervention in boys aged 3-6 years, while emotional symptoms ( = 0.048) in girls. Finally, improvement on peer problems ( = 0.005) was observed in participants with high TDS. The findings suggest that community-based family workshop intervention can improve social adaptation of rural LBC. Moreover, this effect was different in boys and girls and also affected by age and baseline total difficulties. The results indicated that community-based family workshop intervention can be implemented in rural China to improve mental and behavioral health among LBC in the future.
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http://dx.doi.org/10.3389/fpubh.2020.506191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759677PMC
May 2021

In vivo biocompatibility evaluation of Zn-0.05Mg-(0, 0.5, 1wt%)Ag implants in New Zealand rabbits.

Mater Sci Eng C Mater Biol Appl 2021 Feb 25;119:111435. Epub 2020 Aug 25.

Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China. Electronic address:

Bio-absorbable Zn alloys have been attractive replacements for the traditionally permanent implants due to their reasonable mechanical strength and elongation, degradation rate, and biocompatibility. The hybridization addition of Mg and Ag elements could greatly improve the mechanical properties and antibacterial ability of Zn, respectively. In the present paper, in vivo biocompatibility for the Zn-0.05Mg-(0, 0.5, 1 wt%) Ag implants in New Zealand rabbit was qualitatively evaluated during the implantation periods of 4, 12, and 24 weeks. The blood serum biochemical parameters and in vivo integrity of the implants in the live rabbits were monitored by using clinical chemistry analyzing and X-ray radiographic imaging techniques during the implantation process, respectively. There is no great difference in the serum biochemical indicator between the implanted rabbits and the control group. Especially the levels of serum Zn and serum Mg normalize after implantation of 24 weeks. The interfacial adherence between the implants and newly formed bones, and the histopathological morphology of heart, liver, and kidney were observed morphologically under the microscope. The new bones formed and grew surrounding the implants after 12 weeks' post-operation, which were well joined with the original cortical bones after post-implantation of 24 weeks. The heart, liver and kidney were not negatively influenced as evidenced from the serum biochemical indicators and morphologies of the tissues. Zn-0.05Mg-(0, 0.5, 1 wt%) Ag alloys are proved to be in vivo biocompatible and potential candidates for the biodegradable medical implants.
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http://dx.doi.org/10.1016/j.msec.2020.111435DOI Listing
February 2021

Effects of Chemical and Mechanical Lysis on Microbial DNA Yield, Integrity, and Downstream Amplicon Sequencing of Rumen Bacteria and Protozoa.

Front Microbiol 2020 16;11:581227. Epub 2020 Nov 16.

CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.

QIAamp Fast DNA Stool Mini Kit (QIAGEN, Valencia, CA, United States) and RBB + C (Yu and Morrison, 2004) methodologies are widely employed to extract microbial DNA from rumen samples and can exhibit different efficiencies of obtaining DNA yield, quality, and downstream amplicon sequence analysis. No study has conducted to investigate the contributions of chemical and mechanical lysis on DNA extraction, which included chemical lysis from QIAamp Fast DNA Stool Mini Kit (QIA) and RBB + C (YM), bead (BB), and sand beating (SB). Effects of chemical lysis and bead beating (BB) were investigated by conducting a 2 × 2 factorial-designed experiment with four methodologies, including QIA without (QIA-) and with BB (QIA + BB), and YM without (YM-) and with BB (YM + BB). Comparisons between bead and sand were conducted by comparing methodologies of YM + BB and YM + SB. Comparing with QIA, YM had lower ( ≤ 0.10) OD and diversity of ZOTUs and length polymorphism of protozoal amplicons but harvested greater ( ≤ 0.086) DNA from fibrolytic bacteria such as lineages. Including BB increased ( = 0.001) total DNA yield without affecting ( ≥ 0.55) OD and richness of bacterial ZOTUs but decreased ( ≤ 0.08) richness of both ZOTUs and length polymorphism of protozoal amplicon. Bead beating and SB showed no difference ( ≥ 0.19) in DNA yield and quality and bacterial and protozoal community. In summary, chemical lysis provided by RBB + C and QIAamp Fast DNA Stool Mini Kit should be better to extract DNA for analyzing bacterial and protozoal community, respectively. Sand can be an alternative beater for DNA extraction, and mechanical lysis is not recommended for protozoal community analysis.
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http://dx.doi.org/10.3389/fmicb.2020.581227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701101PMC
November 2020

Accurate assessment of parabens exposure in healthy Chinese female adults: Findings from a multi-pathway exposure assessment coupled with intervention study.

Environ Res 2021 02 27;193:110540. Epub 2020 Nov 27.

State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei, 430030, China. Electronic address:

Exposure of humans to parabens is widespread and urinary parabens are widely used as exposure biomarkers. However, are the levels of these chemicals suitable to assess exposure to parabens? We conducted an intervention study by controlling the use of personal care products (PCPs) to explore the exposure of parabens. Ten female participants were recruited who were treated with different types of PCPs during the 18-day study period. The concentrations of parabens and their metabolites in matrices of different exposure pathways (dust, drinking water and dietary food) and urine samples were determined. We demonstrated that PCPs were the major sources of parabens, accounting for >99% of total exposure. The metabolites were nonspecific to individual parabens and could not be used as exposure biomarkers. Urinary paraben concentrations were positively correlated with external exposure levels. However, poor reproducibility was observed, with intraclass correlation coefficients (ICC) ranging from 0.125 to 0.295 in unadjusted urinary concentrations. Creatinine-adjusting could not significantly improve the ICC values in random spot samples. After adjusting for both creatinine and kinetic models, the ICC values ranged from 0.695 to 0.886, indicating a good reproducibility. So, toxicokinetic parameters may be taken into consideration for precise monitoring of exposures for the non-persistent pollutants.
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http://dx.doi.org/10.1016/j.envres.2020.110540DOI Listing
February 2021

Association of digestive symptoms with severity and mortality of COVID-19: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Oct;99(43):e22736

Department of Nursing.

Background: Gastrointestinal manifestations are common in patients with COVID-19, but the association between specific digestive symptoms and COVID-19 prognosis remains unclear. This study aims to assess whether digestive symptoms are associated with COVID-19 severity and mortality.

Methods: We will search PubMed, Embase, Web of Science, and the Cochrane Central Register of Controlled Trials up to September, 2020, to identify studies that compared the prevalence of at least one specific digestive symptom between severe and non-severe COVID-19 patients or between non-survivors and survivors. Two independent reviewers will assess the risk of bias of the included cohort studies using the modified Newcastle-Ottawa Scale. Meta-analyses will be conducted to estimate the pooled prevalence of individual symptoms using the inverse variance method with the random-effects model. We will conduct subgroup analyses, sensitivity analyses, and meta-regression analyses to explore the sources of heterogeneity. The Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach will be used to assess the quality of the evidence.

Results: The results of this study will be published in a peer-reviewed journal.

Conclusion: Our meta-analysis will comprehensively evaluate the association between different digestive symptoms and the severity and mortality of patients infected with COVID-19. This study will provide evidence to help determine whether special protective measures and treatment options are needed for patients with digestive system comorbidities during the COVID-19 pandemic.

Inplasy Registration Number: INPLASY202090055.
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http://dx.doi.org/10.1097/MD.0000000000022736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581056PMC
October 2020

Effect of medically lowering intraocular pressure in glaucoma suspects with high myopia (GSHM study): study protocol for a randomized controlled trial.

Trials 2020 Sep 29;21(1):813. Epub 2020 Sep 29.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, No.7, Jinsui Road, Guangzhou, 510060, China.

Background: Currently, whether and when intraocular pressure (IOP)-lowering medication should be used in glaucoma suspects with high myopia (GSHM) remains unknown. Glaucoma suspects are visual field (VF) defects that cannot be explained by myopic macular changes or other retinal and neurologic conditions. Glaucoma progression is defined by VF deterioration. Here we describe the rationale, design, and methodology of a randomized controlled trial (RCT) designed to evaluate the effects of medically lowering IOP in GSHM (GSHM study).

Methods: The GSHM study is an open-label, single-center, RCT for GSHM. Overall, 264 newly diagnosed participants, aged 35 to 65 years, will be recruited at the Zhongshan Ophthalmic Center, Sun Yat-sen University, between 2020 and 2021. Participants will be randomly divided into two arms at a 1:1 ratio. Participants in the intervention arm will receive IOP-lowering medication, while participants in the control arm will be followed up without treatment for 36 months or until they reach the end point. Only one eye per participant will be eligible for the study. If both eyes are eligible, the eye with the worse VF will be recruited. The primary outcome is the incidence of glaucoma suspect progression by VF testing over 36 months. The secondary outcomes include the incidence of changes in the optic nerve head morphology including the retinal nerve fiber layer, and retinal ganglion cell-inner plexiform layer loss, progression of myopic maculopathy, visual function loss, and change in the quality of life. Statistical analyses will include baseline characteristics comparison between the intervention and control groups using a two-sample t-test and Wilcoxon rank sum test; generalized linear models with Poisson regression for the primary outcome; Kaplan-Meier curve and log-rank test for the incidence of the secondary outcome; and longitudinal analyses to assess trends in outcomes across time.

Discussion: To the best of our knowledge, the GSHM study is the first RCT to investigate the impact of medically lowering IOP in GSHM. The results will have implications for the clinical management of GSHM.

Trial Registration: ClinicalTrials.gov NCT04296916 . Registered on 4 March 2020.
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http://dx.doi.org/10.1186/s13063-020-04748-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525951PMC
September 2020
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