Publications by authors named "Xiu Liu"

234 Publications

Author Correction: MicroRNA-302a promotes neointimal formation following carotid artery injury in mice by targeting PHLPP2 thus increasing Akt signaling.

Acta Pharmacol Sin 2021 Dec 1. Epub 2021 Dec 1.

Department of Pharmacology, and Cardiac & Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.

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http://dx.doi.org/10.1038/s41401-021-00815-0DOI Listing
December 2021

Introduction of a Cys360Tyr Mutation in ANO5 Creates a Mouse Model for Gnathodiaphyseal Dysplasia.

J Bone Miner Res 2021 Nov 29. Epub 2021 Nov 29.

Beijing Institute of Dental Research, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.

Gnathodiaphyseal dysplasia (GDD) is a rare autosomal dominant genetic disease characterized by the osteosclerosis of tubular bones and the formation of cemento-osseous lesions in mandibles. Although genetic mutations for GDD have been identified in the ANO5/TMEM16E gene, the cellular and molecular mechanisms behind the pathogenesis of GDD remain unclear. Here, we generated the first knock-in mouse model for GDD with the expression of human mutation p.Cys360Tyr in ANO5. Homozygous Ano5 knock-in mice (Ano5 ) replicated GDD-like skeletal features, including massive jawbones, bowing tibia, bone fragility, sclerosis, and cortical thickening of the femoral and tibial diaphysis. Serum alkaline phosphatase (ALP) levels were elevated in Ano5 mice as in GDD patients with p.Cys360Tyr mutation. Calvaria-derived Ano5 osteoblast cultures showed increased osteoblastogenesis, including hypermineralized bone matrix and enhanced bone formation-related factors expression. Interestingly, Ano5 bone marrow-derived macrophage cultures showed decreased osteoclastogenesis, and Ano5 osteoclasts exhibited disrupted actin ring formation, which may be associated with some signaling pathways. In conclusion, this new mouse model may facilitate elucidation of the pathogenesis of GDD and shed more light on its treatment. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jbmr.4481DOI Listing
November 2021

Two novel human anti-CD25 antibodies with antitumor activity inversely related to their affinity and in vitro activity.

Sci Rep 2021 Nov 25;11(1):22966. Epub 2021 Nov 25.

Antibody Research and Development Center, Shandong Boan Biotechnology Co., Ltd., Yantai, 264670, People's Republic of China.

High tumor regulatory T (Treg) cell infiltration is associated with poor prognosis of many cancers. CD25 is highly expressed on tumor Treg cells and is a potential target for Treg deletion. Previously characterized anti-CD25 antibodies appear to have limited efficacy in tumor inhibition. Here we identified two human anti-CD25 antibodies, BA9 and BT942, which did not prevent the activation of IL-2R signaling pathway by IL-2. BT942 had weaker binding and cytotoxic activity to human CD25-expressing cell lines than BA9. But both demonstrated significant tumor growth inhibition in early and late-stage animal cancer models. BT942 resulted in a higher expansion of CD8 T cells and CD4 T cells in tumor microenvironment in mouse MC38 model compared to BA9. BT942 also demonstrated significant higher tumor growth inhibition and higher expansion of CD8 T cells and CD4 T cells in combination with an anti-PD1 antibody. Pharmacokinetic study of BT942 in cynomolgus monkeys demonstrated a half-life of 206.97 ± 19.03 h. Structural analysis by cryo-EM revealed that BT942 recognizes an epitope on opposite side of the CD25-IL-2 binding site, consistent with no IL-2 signaling blockade in vitro. BT942 appears to be an excellent candidate for cancer immunotherapy.
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http://dx.doi.org/10.1038/s41598-021-02449-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617198PMC
November 2021

Circulating Natural Autoantibodies to HER2-Derived Peptides Performed Antitumor Effects on Oral Squamous Cell Carcinoma.

Front Pharmacol 2021 5;12:693989. Epub 2021 Nov 5.

Beijing Institute of Dental Research, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.

Natural autoantibodies play a crucial role in destruction of malignant tumors due to immune surveillance function. Epidermal growth factor receptor 2 (HER2) has been found to be highly expressed in a variety of epithelial tumors including oral squamous cell carcinoma (OSCC). The present study was thus undertaken to investigate the effect of anti-HER2 natural autoantibodies on OSCC. Compared with cancer-adjacent tissues, cancer tissues from OSCC patients exhibited higher HER2 expression especially in those with middle & advanced stage OSCC. Plasma anti-HER2 IgG levels examined with an enzyme-linked immunosorbent assay (ELISA) developed in-house showed differences between control subjects, individuals with oral benign tumor and patients with OSCC. In addition, anti-HER2 IgG-abundant plasma was screened from healthy donors to treat OSCC cells and to prepare for anti-HER2 intravenous immunoglobulin (IVIg). Both anti-HER2 IgG-abundant plasma and anti-HER2 IVIg could significantly inhibit proliferation and invasion of OSCC cells by inducing the apoptosis, and also regulate apoptosis-associated factors and epithelial-mesenchymal transition (EMT), respectively. Besides, the complement-dependent cytotoxicity (CDC) pathway was likely to contribute to the anti-HER2 IgG mediated inhibition of OSCC cells. After the HER2 gene was knocked down with HER2-specific siRNAs, the inhibitory effects on OSCC cell proliferation and apoptotic induction faded away. In conclusion, human plasma IgG, or IVIg against HER2 may be a promising agent for anti-OSCC therapy.
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http://dx.doi.org/10.3389/fphar.2021.693989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602057PMC
November 2021

Extracellular Vesicle-Mediated Secretion of HLA-E by Trophoblasts Maintains Pregnancy by Regulating the Metabolism of Decidual NK Cells.

Int J Biol Sci 2021 22;17(15):4377-4395. Epub 2021 Oct 22.

Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310016, Hangzhou, China.

Extracellular vesicles derived from trophoblasts (T-EVs) play an important role in pregnancy, but the mechanism is not entirely clear. In this study, we found that HLA-E, which is mostly confined to the cytoplasm of trophoblast cells, was secreted by T-EVs. The level of HLA-E in T-EVs from unexplained recurrent spontaneous abortion (URSA) patients was lower than that in normal pregnancy (NP) and RSA patients who had an abnormal embryo karyotype (AK-RSA). T-EVs promoted secretion of IFN-γ and VEGFα by decidual NK (dNK) cells from URSA patients via HLA-E, VEGFα was necessary for angiogenesis and trophoblast growth, and IFN-γ inhibited Th17 induction. Glycolysis and oxidative phosphorylation (OxPhos) were involved in this process. Glycolysis but not OxPhos of dNK cells facilitated by T-EVs was dependent on mTORC1 activation. Inhibition of T-EV production increased the susceptibility of mice to embryo absorption, which was reversed by transferring exogenous T-EVs. T-EVs promoted secretion of IFN-γ and VEGFα by dNK cells to maintain pregnancy via Qa-1 in abortion-prone mouse models. This study reveals a new mechanism of pregnancy maintenance mediated by HLA-E via T-EVs.
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http://dx.doi.org/10.7150/ijbs.63390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579460PMC
October 2021

High loading BaTiO nanoparticles chemically bonded with fluorinated silicone rubber for largely enhanced dielectric properties of polymer nanocomposites.

Phys Chem Chem Phys 2021 Dec 1;23(46):26219-26226. Epub 2021 Dec 1.

State Key Laboratory of Environment-friendly Energy Materials, School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.

Integrating high-loading dielectric nanoparticles into polar polymer matrices potentially can profit the intrinsic polarization of each phase and allow for greatly enhanced dielectric properties in polymer nanocomposites. It is however challenging to achieve desirable highly filled polar polymer composites because of the lack of efficient approaches to disperse nanoparticles and maintain interfacial compatibility. Here, we report a versatile route to fabricate highly filled barium titanate/fluorinated silicone rubber (BT/FSR) nanocomposites by "thiol-ene click" and isostatic pressing techniques. The loaded BT nanoparticles (from 82 wt% to 90 wt%) are chemically bonded with FSR in the nanocomposites. The existence of the polar group (-CHCF) of the polymer matrix does not affect the uniform dispersion of the nanoparticles or the good interfacial compatibility. The 90 wt% BT/FSR nanocomposite shows the highest dielectric constant of 57.8 at 10 Hz, while the loss tangent can be kept below 0.03. Besides, BT/FSR nanocomposites display higher breakdown strength than BT/SR nanocomposites. This work offers a facile strategy towards superior dielectric properties of polymer nanocomposites.
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http://dx.doi.org/10.1039/d1cp04040eDOI Listing
December 2021

Effects of Slaughter Age on Myosin Heavy Chain Isoforms, Muscle Fibers, Fatty Acids, and Meat Quality in Muscle of Tibetan Sheep.

Front Vet Sci 2021 26;8:689589. Epub 2021 Oct 26.

Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.

Tibetan sheep is one of the dominant livestock at Qinghai-Tibet Plateau, which is the main food source of local people. In order to investigate the effect of slaughter age on meat quality, fatty acid profile and expression of myosin heavy chain (MyHC) isoform genes were analyzed in Tibetan sheep. A total of 24 Tibetan sheep including 4 months old (4 m), 1.5 years old (1.5 y), 3.5 years old (3.5 y), and 6 years old (6 y) were randomly selected. The results indicated that the MyHC IIx and MyHC IIb mRNAs increased with age, whereas MyHC IIa mRNA decreased. MyHC I mRNA was highest at 3.5 y. There were differences in the muscle fiber types of Tibetan sheep at different ages. Intramuscular fat (IMF) was highest at 1.5 y, the pH and pH value of 6 y sheep were lower than the other groups, the shear force increased with age ( < 0.05), and drip loss increased with age ( < 0.01). Tibetan sheep at 1.5 y had lower saturated fatty acid (SFA) contents and higher monounsaturated fatty acid (MUFA) contents ( < 0.05). Different muscle fiber types influence the meat quality and fatty acid composition of Tibetan sheep with increasing age. These results demonstrated the effect of age on meat quality of Tibetan sheep through regulation of expression of the MyHC isoforms which changed the myofiber types, and 1.5 y Tibetan sheep meat was more suitable for a healthy human diet.
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http://dx.doi.org/10.3389/fvets.2021.689589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576190PMC
October 2021

Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12a.

Anal Bioanal Chem 2021 Oct 25. Epub 2021 Oct 25.

Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin, China.

In this study, Lba Cas12a (Cpf1) as one of the CRISPR systems from Lachnospiraceae bacterium was coupled with a hybridization chain reaction (HCR) to develop an electrochemical biosensor for detecting the pathogenic bacterium, Salmonella typhimurium. Autonomous cross-opening of functional DNA hairpin structures of HCR yielded polymer double-stranded DNA wires consisting of numerous single-stranded DNAs, which initiated the trans-cleavage activity of CRISPR-Cas12a to indiscriminately cleave random single-stranded DNA labeling electrochemical tags on the surface of the electrode. It led to a variation in the electron transfer of electrochemical tags. The polymer double-stranded DNA of HCR was immobilized on dynabeads (DBs) via the S. typhimurium aptamer and released from DBs. The established method could selectively and sensitively quantify S. typhimurium in samples with detection limits of 20 CFU/mL. Our study provides a novel insight for exploring universal analytical methods for pathogenic bacteria based on CRISPR-Cas12a coupled with HCR.
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http://dx.doi.org/10.1007/s00216-021-03733-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542504PMC
October 2021

Altered Hypoxia-Induced and Heat Shock Protein Immunostaining in Secondary Hair Follicles Associated with Changes in Altitude and Temperature in Tibetan Cashmere Goats.

Animals (Basel) 2021 Sep 25;11(10). Epub 2021 Sep 25.

School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.

This experiment compared secondary hair follicles (SFs) in Tibetan cashmere goats from two different steppes that were at different altitudes and had different temperatures. Twenty-four 2-year-old goats were studied. Twelve goats were from Rikaze in Tibet which is at an altitude of above 5000 m with an average temperature of 0 °C. The other 12 studied goats were from Huan County of Gansu Province which is around 2000 m above sea level with an average temperature of 9.2 °C. The structural features of SFs were assessed using light microscopy and transmission electron microscopy. The presence of HIF-1a, HIF-2a, HIF-3a, HSP27, and HOXC13 proteins was studied using immunohistochemistry and immunofluorescence. Light and electron microscopy revealed that the SFs of the Tibetan cashmere goats that lived in the Rikaze Steppe were in the proanagen stage in May. However, the SFs of the goats from the lower warmer Huan County were in the anagen stage at the same time. Immunohistochemistry revealed intense immunostaining for HIF-1a protein in the inner root sheath (IRS) and hair shaft (HS); immunostaining against HIF-2a in the outer root sheath (ORS) and IRS; HIF-3a protein immunostaining in the ORS; HSP27 immunostaining in the ORS, IRS, and HS; and HOXC13 immunostaining in the ORS and HS. HIF-1a protein expression in the IRS and HS was higher than the expression in the ORS ( < 0.05) while the expression of HIF-2a protein was higher in the ORS and IRS than the HS ( < 0.05). The expression of HIF-3a protein was higher in the ORS than in the IRS ( < 0.05). Expression of HOXC13 protein was higher in the ORS than in the IRS and HS ( < 0.05). Immunostaining of HIF-1a, HIF-2a, and HSP27 protein was significantly higher in SFs from cashmere goats from Rikaze than in goats from Huan ( < 0.05). In contrast, HOX13 protein immunostaining was significantly higher in cashmere goats from Huan than from Rikaze ( < 0.05). Significant differences were observed in the SFs of cashmere goats from two locations that differ in altitude and temperature. This suggests the differences in the secondary hair follicles could be due to the hypoxia and lower temperatures experienced by the goats in Rikaze. These results are useful in understanding how altitude and temperature influence SF development. Hair produced by the SFs are used for down fiber. Therefore, understanding of the factors that influence SF development will allow the production and harvest of these valuable fibers to be maximized.
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http://dx.doi.org/10.3390/ani11102798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532893PMC
September 2021

LATE MERISTEM IDENTITY1 regulates leaf margin development via the auxin transporter gene SMOOTH LEAF MARGIN1.

Plant Physiol 2021 Sep;187(1):218-235

The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.

Plant leaves have evolved into diverse shapes and LATE MERISTEM IDENTITY1 (LMI1) and its putative paralogous genes encode homeodomain leucine zipper transcription factors that are proposed evolutionary hotspots for the regulation of leaf development in plants. However, the LMI1-mediated regulatory mechanism underlying leaf shape formation is largely unknown. MtLMI1a and MtLMI1b are putative orthologs of LMI1 in the model legume barrelclover (Medicago truncatula). Here, we investigated the role of MtLMI1a and MtLMI1b in leaf margin morphogenesis by characterizing loss-of-function mutants. MtLMI1a and MtLMI1b are expressed along leaf margin in a near-complementary pattern, and they redundantly promote development of leaf margin serrations, as revealed by the relatively smooth leaf margin in their double mutants. Moreover, MtLMI1s directly activate expression of SMOOTH LEAF MARGIN1 (SLM1), which encodes an auxin efflux carrier, thereby regulating auxin distribution along the leaf margin. Further analysis indicates that MtLMI1s genetically interact with NO APICAL MERISTEM (MtNAM) and the ARGONAUTE7 (MtAGO7)-mediated trans-acting short interfering RNA3 (TAS3 ta-siRNA) pathway to develop the final leaf margin shape. The participation of MtLMI1s in auxin-dependent leaf margin formation is interesting in the context of functional conservation. Furthermore, the diverse expression patterns of LMI1s and their putative paralogs within key domains are important drivers for functional specialization, despite their functional equivalency among species.
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http://dx.doi.org/10.1093/plphys/kiab268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418409PMC
September 2021

MicroRNA-432 inhibits milk fat synthesis by targeting and in ovine mammary epithelial cells.

Food Funct 2021 Oct 4;12(19):9432-9442. Epub 2021 Oct 4.

Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.

The microRNA (miR)-432 is differentially expressed in the mammary gland of two breeds of lactating sheep with different milk production traits, and between the non-lactating and peak-lactation periods, but there have been no reports describing the molecular mechanisms involved. In this study, the effect of miR-432 on the proliferation of ovine mammary epithelial cells (OMECs) and the target genes of miR-432 were investigated. The effects of miR-432 on the expression of the target genes and the content of triglycerides in the OMECs were also analyzed. Transfection with a miR-432 mimic was found using CCK8 and Edu assays, to inhibit the viability of OMECs and reduce the number of proliferated OMECs. In contrast, a miR-432 inhibitor had the opposite effect to the miR-432 mimic, and together these results suggest that miR-432 inhibits the proliferation of OMECs. A dual luciferase assay revealed that the genes for stearoyl-CoA desaturase () and lipoprotein lipase () are targeted by miR-432. The transfection of miR-432 mimic into OMECs resulted in decreases in the expression of and , and three other milk fat synthesis marker genes; , and . The mimic also decreased the content of triglycerides. The miR-432 inhibitor had the opposite effect to the mimic on the expression of these genes and the level of triglycerides. This is the first study to reveal the biological mechanisms by which miR-432 inhibits milk fat synthesis in sheep.
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http://dx.doi.org/10.1039/d1fo01260fDOI Listing
October 2021

Macrophage NFATc3 prevents foam cell formation and atherosclerosis: evidence and mechanisms.

Eur Heart J 2021 Sep 27. Epub 2021 Sep 27.

Program of Kidney and Cardiovascular Diseases, the Fifth Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, 74 Zhongshan 2 Rd, Guangzhou 510080, China.

Aims: Our previous study demonstrated that Ca2+ influx through the Orai1 store-operated Ca2+ channel in macrophages contributes to foam cell formation and atherosclerosis via the calcineurin-ASK1 pathway, not the classical calcineurin-nuclear factor of activated T-cell (NFAT) pathway. Moreover, up-regulation of NFATc3 in macrophages inhibits foam cell formation, suggesting that macrophage NFATc3 is a negative regulator of atherogenesis. Hence, this study investigated the precise role of macrophage NFATc3 in atherogenesis.

Methods And Results: Macrophage-specific NFATc3 knockout mice were generated to determine the effect of NFATc3 on atherosclerosis in a mouse model of adeno-associated virus-mutant PCSK9-induced atherosclerosis. NFATc3 expression was decreased in macrophages within human and mouse atherosclerotic lesions. Moreover, NFATc3 levels in peripheral blood mononuclear cells from atherosclerotic patients were negatively associated with plaque instability. Furthermore, macrophage-specific ablation of NFATc3 in mice led to the atherosclerotic plaque formation, whereas macrophage-specific NFATc3 transgenic mice exhibited the opposite phenotype. NFATc3 deficiency in macrophages promoted foam cell formation by potentiating SR-A- and CD36-meditated lipid uptake. NFATc3 directly targeted and transcriptionally up-regulated miR-204 levels. Mature miR-204-5p suppressed SR-A expression via canonical regulation. Unexpectedly, miR-204-3p localized in the nucleus and inhibited CD36 transcription. Restoration of miR-204 abolished the proatherogenic phenotype observed in the macrophage-specific NFATc3 knockout mice, and blockade of miR-204 function reversed the beneficial effects of NFATc3 in macrophages.

Conclusion: Macrophage NFATc3 up-regulates miR-204 to reduce SR-A and CD36 levels, thereby preventing foam cell formation and atherosclerosis, indicating that the NFATc3/miR-204 axis may be a potential therapeutic target against atherosclerosis.
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http://dx.doi.org/10.1093/eurheartj/ehab660DOI Listing
September 2021

ALK Rearrangement-Positive Pancreatic Cancer with Brain Metastasis Has Remarkable Response to ALK Inhibitors: A Case Report.

Front Oncol 2021 6;11:724815. Epub 2021 Sep 6.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Patients with metastatic pancreatic cancer typically have poor prognosis due to the limited effectiveness of existing treatment options. ALK rearrangement-positive is rare in pancreatic cancer, but may occur in those with KRAS-wild type. We present a 34-year-old young man with ALK rearrangement-positive and KRAS-wild pancreatic cancer who had a remarkable response to crizotinib after resistance to prior chemotherapy and re-response to alectinib after brain metastases developed. This clinical observation suggests that comprehensive molecular profiling to guide targeted therapies is not only feasible, but also significantly improves survival outcomes for a subgroup of patients with pancreatic cancer.
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http://dx.doi.org/10.3389/fonc.2021.724815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456297PMC
September 2021

Influencing factors and strategies of enhancing nanoparticles into tumors .

Acta Pharm Sin B 2021 Aug 24;11(8):2265-2285. Epub 2021 Mar 24.

Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 117004, China.

The administration of nanoparticles (NPs) first faces the challenges of evading renal filtration and clearance of reticuloendothelial system (RES). After that, NPs infiltrate through the expanded endothelial space and penetrated the dense stroma of tumor microenvironment to tumor cells. As long as possible to prolong the time of NPs remaining in tumor tissue, NPs release active agent and induce pharmacological action. This review provides a comprehensive summary of the physical and chemical properties of NPs and the influence of various biological factors in tumor microenvironment, and discusses how to improve the final efficacy through adjusting the characteristics and structure of NPs. Perspectives and future directions are also provided.
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http://dx.doi.org/10.1016/j.apsb.2021.03.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424218PMC
August 2021

Prevalence of celiac disease in China: Meta-analysis and serological survey in high-risk populations.

J Dig Dis 2021 Nov 3;22(11):645-655. Epub 2021 Nov 3.

Department of Gastroenterology, State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.

Objective: To summarize data from a serological survey of high-risk populations in Guangdong Province, China, and to perform a meta-analysis to investigate the prevalence and seroprevalence of celiac disease (CD) in the Chinese general and high-risk populations.

Methods: We collected data from the serological survey of high-risk population of CD in Guangdong Province, China (N = 1390) by testing their serum tissue transglutaminase immunoglobulin A (tTG-IgA), deamidated gliadin peptides immunoglobulin A (DGP-IgA) and deamidated gliadin peptides immunoglobulin G (DGP-IgG). Additionally, a literature search was performed on PubMed, EMBASE, Cochrane Library and three Chinese databases for articles published up to 20 December 2020 to estimate the pooled prevalence and seroprevalence of CD in China.

Results: In the serological survey, 0.94% (13/1390) of individuals were positive for CD antibodies. In a meta-analysis of 18 studies, the seroprevalence of CD in the general Chinese population was 0.27% (95% confidence interval [CI] 0.02%-0.71%). While that in the high-risk population was 8.34% (95% CI 4.90%-12.54%) (odds ratio 7.27, 95% CI 4.06-13.04). The prevalence of biopsy-confirmed CD in high-risk Chinese populations was 4.44% (95% CI 1.53%-8.58%). The seroprevalence of CD varied with patients' geographical origin, being higher in northern China than in southern China.

Conclusions: Early diagnosis of CD by serological screening in high-risk population and generous serological testing in those with vague symptoms, especially in northern China, are recommended.
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http://dx.doi.org/10.1111/1751-2980.13049DOI Listing
November 2021

Regulating glycolysis and heat shock proteins in Gannan yaks ( ) in response to hypoxia of the Qinghai-Tibet Plateau.

Arch Anim Breed 2021 19;64(2):345-353. Epub 2021 Aug 19.

Faculty of Animal Science and Technology, Gansu Agricultural University, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Lanzhou 730070, China.

Glycolysis and heat shock proteins (HSPs) play an important role in hypoxia-intolerant species during hypoxia conditions. This study was conducted to evaluate the differences of glycolysis and heat shock proteins (HSPs) in Gannan yaks (), with the main goal of understanding how the response to hypoxia changes with altitude. Here, the genes and enzymes of glycolysis and HSPs were detected in heart, liver, lung, kidney, and longissimus dorsi from Gannan yaks at different altitude (2500 and 3500  ) using qPCR, western blot, and enzyme kits. The results showed that the expression of and was increased with altitude ( ) in above tissues. Significantly increased lactate dehydrogenase (LDH), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide (NADH) levels and the ratio of NADH/NAD were also observed in heart, lung, and longissimus dorsi tissues ( ), as well as a decreased citric acid (CA) level ( ). Furthermore, we observed significant global increases in the protein and mRNA expression levels of both the ATP-independent HSP27 and the ATP-dependent HSP60 during hypoxic conditions ( ). These findings revealed that hypoxia-reprogrammed glucose metabolism promotes energy supply via up-regulated glycolysis and weakness of the tricarboxylic acid (TCA) cycle. HSPs were activated and the prioritization of cytoprotective protein chaperone functions over energy conservation in yak under hypoxic conditions. These results are useful to better understand the unique adaptability of yak, allowing them to survive in hypoxia conditions.
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http://dx.doi.org/10.5194/aab-64-345-2021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386194PMC
August 2021

MicroRNA-200b Regulates the Proliferation and Differentiation of Ovine Preadipocytes by Targeting and .

Animals (Basel) 2021 Aug 17;11(8). Epub 2021 Aug 17.

Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.

MicroRNAs (miRNAs) are crucial regulatory molecules in lipid deposition and metabolism. However, the effect of miR-200b on the regulation of proliferation and adipogenesis of ovine preadipocytes is unknown in the sheep (). In this study, the expression profiles of miR-200b were investigated in the seven tissues of Tibetan ewes and differentiated preadipocytes. The effect of miR-200b, as well as its target genes and , on the proliferation of ovine preadipocytes and adipogenesis was also investigated, using cell viability analysis, EdU staining, Oil Red O staining and reverse transcription-quantitative PCR (RT-qRCR). The miR-200b was expressed in all the tissues investigated, and it was highly expressed in lung, liver, subcutaneous adipose and spleen tissues. The expression of miR-200b continuously decreased when the differentiation of ovine preadipocytes initiated. The miR-200b mimic dramatically accelerated the proliferation but inhibited differentiation of ovine preadipocytes. The miR-200b inhibitor resulted in an opposite effect on the proliferation and differentiation of ovine preadipocytes. The dual luciferase reporter assay results showed that miR-200b mimic significantly decreased the luciferase activity of and in HEK293 cells transfected with wild-type dual luciferase reporter vectors. This suggests that and are the target genes of miR-200b. In over-expressed- preadipocytes, the number of EdU-labeled preadipocytes and the expression levels of proliferation marker genes , , and significantly decreased. In addition, the transfection of over-expressed- vector into adipocytes remarkably increased the accumulation of lipid droplets and the expression levels of differentiation marker genes , , and . These results suggest that miR-200b accelerated the proliferation but inhibited the adipogenic differentiation of ovine preadipocytes by targeting and , respectively.
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http://dx.doi.org/10.3390/ani11082417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388755PMC
August 2021

Estrogen inhibits the growth of colon cancer in mice through reversing extracellular vesicle-mediated immunosuppressive tumor microenvironment.

Cancer Lett 2021 Nov 13;520:332-343. Epub 2021 Aug 13.

Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310016, Hangzhou, China; Department of Obstetrics and Gynecology, Key Laboratory of Reproductive Dysfunction, Management of Zhejiang Province, 310016, Hangzhou, China. Electronic address:

Postmenopausal women taking estrogen supplements are at a lower risk of advanced colorectal cancer, but the underlying mechanism remains unclear. Thus, this study examined the role of estrogen in colorectal cancer. Estrogen receptor expression levels in in situ colorectal cancer tissue from female patients increased significantly, indicating their estrogen sensitivity. Compared with the sham-operated group, the growth of MC38 tumors was enhanced in ovariectomized mice, which was reversed in ovariectomized mice with E2 supplementation. The PD-L1 M2-like macrophage, regulatory T (Treg) cell, and myeloid-derived suppressor cell (MDSC) populations significantly increased, and the population of cytotoxic CD8 T cells declined in MC38 tumors in ovariectomized mice, which were all reversed in ovariectomized mice with E2 supplementation. MC38 cell-derived extracellular vesicles (MC38-EVs), but not EVs derived from MC38 cells treated with E2 (E2-MC38-EVs), were involved in the establishment of immunosuppressive tumor microenvironment. E2-MC38-EVs contained lower TGF-β1 levels and were less capable of inducing Treg cells than MC38-EVs in vitro. Overall, these results show that estrogen treatment prevents MC38 tumor growth via regulating the tumor immune microenvironment through MC38-EVs.
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http://dx.doi.org/10.1016/j.canlet.2021.08.011DOI Listing
November 2021

Effect of glycolysis and heat shock proteins on hypoxia adaptation of Tibetan sheep at different altitude.

Gene 2021 Nov 9;803:145893. Epub 2021 Aug 9.

Faculty of Animal Science and Technology, Gansu Agricultural University, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Lanzhou 730070, China. Electronic address:

Glycolysis and heat shock proteins (HSPs) play an important role in mediating the physiological response to hypoxia. The changes of glycolysis and HSPs with altitude would provide important information regarding ways to prevent hypoxia-related sickness in both animals and humans. In this study, the expression pattern of HIF1A, PDK4, HSP27 and HSP60, indexes activity and content of glucose metabolism were detected in heart, lung, brain, and quadriceps femoris taken from Tibetan sheep (Ovis aries) that were raised at different altitudes (2,500 m, 3,500 m and 4,500 m). The expression of HIF1A and PDK4 was increased with increasing altitude in all of the tissues. The lactate dehydrogenase (LDH) activities and adenosine triphosphate (ATP), nicotinamide adenine dinucleotide (NADH (redox state), NAD), lactic acid (LA), pyruvic acid (PA) contents were all increased with increasing altitude in all of the tissues. The ratio of NADH/NAD and LA/PA were higher in sheep at an altitude of 4,500 m than of 3,500 m and 2,500 m in all tissues, except for the NADH/NAD ratio in lung and quadriceps femoris. An increase in the protein and mRNA expression of ATP-independent HSP27 during hypoxia condition was detected. The expression of ATP-dependent HSP60 mRNA and protein was increased in all of the tissues at an altitude of 3,500 m than of 2,500 m, but was decreased at an altitude of 4,500 m. These results suggest that glycolysis and HSPs are upregulated to ensure energy supply and proteostasis during hypoxia, but energy conservation may be prioritized over cytoprotective protein chaperoning in Tibetan sheep tissues during extreme hypoxia.
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http://dx.doi.org/10.1016/j.gene.2021.145893DOI Listing
November 2021

[The regulatory role of autophagy in rats lung ischemia/reperfusion injury].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2021 Jul;37(4):385-388

Institute of Ischemia-Reperfusion Injury, Wenzhou Medical University, Wenzhou 325035.

To investigate the role of cell autophagy in lung ischemia/reperfusion injury in rats. Forty SD rats were randomly divided into 5 groups (=8): ①Sham operated group (sham group):just open rat chest for 3.5 h; ②Ischemia/reperfusion group (I/R group):after open chest, clamp pulmonary hilus for 0.5h then reperfusion for 3 h; ③Solvent group (DMSO group): intraperitoneal injection of DMSO solution for 1h before operation; ④Autophagic inhibitor group (3-MA group); ⑤Autophagic agonist group (Rap group): intraperitoneal injection of autophagic agonist rapamycin before operation; the rest operations of DMSO, 3-MA and Rap groups are the same as that of I/R group. At the end of the experiment, the rats were killed by euthanasia-killing. The lung tissues were collected and the wet/dry weight ratio (W/D) and total lung water content (TLW) of the lung tissues were detected. The lung tissue structure and cell ultramicro morphology were observed by light microscopy and electron microscopy and the injuried alveolar rate(IAR) was calculated. The autophagy-related protein expressions were detected by Western blot. Compared with sham group, the levels of W/D, TLW and IAR were increased, the expressions of autophagy related protein and p-AMPK, Beclin 1, LC3 II were also increased in other four groups, while the protein expressions of p-mTOR and p62 were decreased significantly (< 0.05 or <0.01). Under the light microscope, the other groups of lung tissue had edema and exudation in varying degrees, the structure of alveoli was disordered, the ultrastructural damage of cells was aggravated under the electron microscope, and autophagosome could be observed. Compared with DMSO group, the expressions of autophagy related protein, the levels of W/D, TLW and IAR in 3-MA group were decreased (<0.05 or <0.01), the edema of lung interstitial was lighter, and less cells were found in alveolar cavity. Ultrastructural damage was also lighter and with less autophagosome. Besides, there was no significant difference among I/R, DMSO and Rap groups (>0.05). Autophagy can be activated during ischemia/reperfusion in rats to induce lung injury.
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http://dx.doi.org/10.12047/j.cjap.6061.2021.019DOI Listing
July 2021

A highly polymorphic caprine keratin-associated protein gene identified and its effect on cashmere traits.

J Anim Sci 2021 Sep;99(9)

Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.

Five keratin-associated protein 6 genes (KRTAP6) have been identified in sheep and variation in some KRTAP6 has been associated with wool fiber diameter-related traits, but none of these homologues have been identified in goats. In this study, we reported the identification of the sheep KRTAP6-5 homologue on goat chromosome 1 and polymerase chain reaction (PCR)-single strand conformation polymorphism analysis in 300 Longdong cashmere goats revealed the existence of 12 variant sequences. Both coding region and 3'UTR of the putative caprine KRTAP6-5 displayed a biggest sequence similarity to ovine KRTAP6-5 gene. This suggested that the gene represents caprine KRTAP6-5 sequences, and these sequences composed 23 genotypes, which was the most polymorphism gene in KRTAPs that have been studied. Among these sequences, 15 nucleotide substitutions and a 24-bp insertion/detection were identified. Variation in goat KRTAP6-5 was associated with variation in mean-fiber diameter, suggesting that KRTAP6-5 is worthy of further study in the context of variation in cashmere traits.
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http://dx.doi.org/10.1093/jas/skab233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442941PMC
September 2021

The Effect of BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing.

Pol J Microbiol 2021 Jun 21;70(2):235-243. Epub 2021 Jun 21.

China National Research Institute of Food and Fermentation Industies Co., Ltd, Beijing, China.

BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in BALB/c mice by 16S rRNA amplicon sequencing. The mice were randomly allocated to the control group and three treatment groups of BW2013 (a low-dose group of 10 CFU/ml, a medium-dose group of 10 CFU/ml, and a high-dose group of 10 CFU/ml). The weight of mice was recorded once a week, and the fecal samples were collected for 16S rRNA amplicon sequencing after 28 days of continuous treatment. Compared with the control group, the body weight gain in the treatment groups was not significant. The 16S rRNA amplicon sequencing analysis showed that both the Chao1 and ACE indexes increased slightly in the medium-dose group compared to the control group, but the difference was not significant. Based on PCoA results, there was no significant difference in β diversity between the treatment groups. Compared to the control group, the abundance of increased in the low-dose group. The abundance of Firmicutes increased in the medium-dose group. At the genus level, the abundance of increased in the low-dose group compared to the control group. The increased abundance of and decreased abundance of was observed in the medium-dose group. Additionally, the abundance of Bacteroides increased, and and decreased in the high-dose group. These results indicated that BW2013 could ameliorate gut microbiota composition, but its effects vary with the dose.
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http://dx.doi.org/10.33073/pjm-2021-022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326986PMC
June 2021

Fabrication of size-controlled hierarchical [email protected] heterostructured cages for enhanced gas-phase CO photoreduction.

J Colloid Interface Sci 2022 Jan 23;605:253-262. Epub 2021 Jul 23.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin 150080, PR China. Electronic address:

Designing and constructing advanced heterojunction architectures are desirable for boosting CO photoreduction performance of semiconductor photocatalysts. Herein, we have prepared hierarchical [email protected] core-shell cages with controlled particle sizes using sequential synthesis of Zeolitic imidazolate (ZIF-8) polyhedrons, ZnS cages, and ZnInS nanosheets on the ZnS polyhedron cages. ZIF-8 polyhedrons are firstly synthesized by a liquid-phase approach. The subsequent sulfidation of the ZIF-8 polyhedrons results in the formation of ZnS polyhedron cages, which act as substrates for fabricating [email protected] core-shell cages by growing ZnInS nanosheets. The size of ZnS cages can be tuned to optimize CO photoreduction performance of hierarchical [email protected] core-shell cages. The synergy of the unique hierarchical core-shell cage-like structure and heterojunction composition endows the hybrid catalyst high incident light utilization, abundant active sites, and effective separation of photoexcited charge carriers. Benefiting from these advantages, the optimized hierarchical [email protected] core-shell cages exhibit enhanced performance for CO photoreduction with the CO yield of 87.43 μmol hg and 84.3% selectivity, which are much superior to those of single ZnInS or ZnS. Upon Au decoration, the CO photoreduction performance of [email protected] core-shell cages is further enhanced because of the Schottky junctions and surface plasmon resonance effect.
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http://dx.doi.org/10.1016/j.jcis.2021.07.093DOI Listing
January 2022

Exsolution of Ni Nanoparticles from A-Site-Deficient Layered Double Perovskites for Dry Reforming of Methane and as an Anode Material for a Solid Oxide Fuel Cell.

ACS Appl Mater Interfaces 2021 Aug 21;13(30):35719-35728. Epub 2021 Jul 21.

Univ Rennes, CNRS, ISCR (Institut des Sciences Chimiques de Rennes) - UMR 6226, F-35000 Rennes, France.

Exsolution is a promising technique to design metal nanoparticles for electrocatalysis and renewable energy. In this work, Ni-doped perovskites, (PrBa)MnNiO with = 0, 0.05, 0.1, and 0.2 (S-PBMNx), were prepared to design exsolution systems as solid oxide fuel cell anodes and for catalysis applications. X-ray diffraction and transmission electron microscopy (TEM) analyses demonstrated that correlating A-site deficiency with Ni content can effectively induce exsolution of all Ni under H atmosphere at ∼ 875 °C, yielding the reduced (exsolved) R-PBMNx materials. On heating the exsolution systems in air, metal incorporation in the oxide lattice did not occur; instead, the Ni nanoparticles oxidized to NiO on the layered perovskite surface. The lowest area-specific resistance (ASR) under wet 5% H/N in symmetrical cells was observed for -PBMN0.2 anode (ASR ∼ 0.64 Ω cm at 850 °C) due to the highest Ni particle density in the -PBMNx series. The best performance for dry reforming of methane (DRM) was also obtained for -PBMN0.2, with CH and CO conversion rates at 11 and 32%, respectively, and the highest production of H (37%). The DRM activity of -PBMN0.2 starts at 800 °C and is sustained for up to at least 5 h operation with little carbon deposition (0.017 g·gcat·h). These results clearly demonstrate that varying Ni-doping in layered double perovskite oxides is an effective strategy to manipulate the electrochemical performance and catalytic activity for energy conversion purposes.
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http://dx.doi.org/10.1021/acsami.1c08158DOI Listing
August 2021

Outbreak Associated with Duck Blood Curd in 2019 in Shunyi District, Beijing, China.

Biomed Environ Sci 2021 Jun;34(6):489-492

Shunyi Center for Disease Control and Prevention, Beijing 101300, China.

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http://dx.doi.org/10.3967/bes2021.067DOI Listing
June 2021

Visual assay of Escherichia coli O157:H7 based on an isothermal strand displacement and hybrid chain reaction amplification strategy.

Anal Methods 2021 08 8;13(30):3379-3385. Epub 2021 Jul 8.

School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China.

Here, we describe a simple, sensitive, and enzyme-free method for visual point-of-care detection of 16S rRNA of Escherichia coli O157:H7 based on an isothermal strand displacement-hybrid chain reaction (ISD-HCR) and lateral flow strip (LFS). In this study, the secondary structure of 16S rRNA of E. coli O157:H7 was unwound by two helper oligonucleotides to expose the single-strand-specific nucleic acid sequence. The free specific sequence promoted the toehold-mediated strand displacement reaction to output a large number of FITC-labeled single-stranded DNA probes (capture probe [CP]). The 3'-end sequence of the reporter probe propagated a chain reaction of hybridization events between the two hairpin probes modified with biotin to form long nicked DNA polymers with multiple biotins (RP-HCR complexes); the free CP and RP-HCR complexes then form CP/RP-HCR complexes. The biotin-labeled double-stranded DNA CP/RP-HCR polymers then introduced numerous streptavidin (SA)-labeled gold nanoparticles (AuNPs) on the LFS. The accumulation of AuNPs produced a characteristic red band, which enabled visual detection of changes in the signal of 16S rRNA of E. coli O157:H7. The current approach could detect E. coli O157:H7 at concentrations as low as 10 CFU mL without instrumentation. This approach thus provides a simple, sensitive, and low-cost tool for point-of-care detection of pathogenic bacteria, especially in resource-limited countries.
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http://dx.doi.org/10.1039/d1ay00644dDOI Listing
August 2021

An electrochemical biosensor for the detection of pathogenic bacteria based on dual signal amplification of Cu(PO)-mediated click chemistry and DNAzymes.

Analyst 2021 Jul;146(15):4841-4847

Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun 130122, China.

A novel electrochemical biosensor for detecting pathogenic bacteria was designed based on specific magnetic separation and highly sensitive click chemistry. Instead of enzyme-antibody conjugates, organic-inorganic hybrid nanoflowers [concanavalin A (Con A)-Cu3(PO4)2] were used as the signal probe of the sandwich structure. The inorganic component, the copper ions of hybrid nanoflowers, was first used to amplify signal transduction for enzyme-free detection. Sodium ascorbate could dissolve Cu3(PO4)2 of the signal probe to produce Cu2+, which was subsequently converted to Cu+, triggering the Cu+-catalyzed alkyne-azide cycloaddition (CuAAC) reaction between azide-functionalized ssDNA (a fragment of the DNAzyme-containing sequence) and alkyne-functionalized ssDNA immobilized onto the electrode surface. As a result, the DNAzyme was immobilized onto the gold electrode, which produced a positive and stable electrical signal. An exceptional linear relationship was observed between the electrical signal and the concentration of Salmonella typhimurium (101-107 CFU mL-1) with a detection limit of 10 CFU mL-1. The developed electrochemical biosensor based on dual signal amplification of Cu3(PO4)2-mediated click chemistry and DNAzymes exhibited good results in detecting S. typhimurium in milk samples.
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http://dx.doi.org/10.1039/d1an00982fDOI Listing
July 2021

Long non-coding RNA SDCBP2-AS1 delays the progression of ovarian cancer via microRNA-100-5p-targeted EPDR1.

World J Surg Oncol 2021 Jul 4;19(1):199. Epub 2021 Jul 4.

Wuhan Wuchang Hospital, Wuhan University of Science and Technology, 505 Luoshi Road, South District, Wuchang Hospital, Hongshan District, Wuhan, 430061, Hubei, China.

Background: Dysregulation of long non-coding RNAs has been implied to connect with cancer progression. This research was to decipher the mechanism of long non-coding RNA SDCBP2-AS1 in ovarian cancer (OC) through regulation of microRNA (miR)-100-5p and ependymin-related protein 1 (EPDR1).

Methods: LncRNA SDCBP2-AS1 and EPDR1 levels in OC were assessed by Gene Expression Profiling Interactive Analysis. lncRNA SDCBP2-AS1, miR-100-5p, and EPDR1 levels in OC tissues and cells were determined. SKOV3 and A2780 cells were transfected with lncRNA SDCBP2-AS1, miR-100-5p, and EPDR1-related plasmids or sequences, and then their functions in cell viability, apoptosis, migration, and invasion were evaluated. The interplay of lncRNA SDCBP2-AS1, miR-100-5p, and EPDR1 was clarified.

Results: LncRNA SDCBP2-AS1 and EPDR1 levels were suppressed whilst miR-100-5p level was elevated in OC. After upregulating lncRNA SDCBP2-AS1 or EPDR1, viability, migration, and invasion of OC cells were impaired, and apoptosis rate was increased. Downregulating EPDR1 or upregulating miR-100-5p partially mitigated upregulated lncRNA SDCBP2-AS1-induced impacts on the biological functions of OC cells. LncRNA SDCBP2-AS1 sponged miR-100-5p, and EPDR1 was targeted by miR-100-5p.

Conclusion: It is illustrated that lncRNA SDCBP2-AS1 regulates EPDR1 by sponge adsorption of miR-100-5p to inhibit the progression of OC.
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http://dx.doi.org/10.1186/s12957-021-02295-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256491PMC
July 2021

Hierarchical Co Se-CdSe/MoSe /CdSe Sandwich-Like Heterostructured Cages for Efficient Photocatalytic CO Reduction.

Small 2021 Aug 23;17(31):e2100412. Epub 2021 Jun 23.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, 150080, P. R. China.

Fabricating efficient photocatalysts with rapid charge carrier separation and high visible light harvesting is an advisable strategy to improve CO reduction performance. Herein, hierarchical Co Se-CdSe/MoSe /CdSe cages with sandwich-like heterostructure are prepared to act as efficient photocatalysts for CO reduction. In this study, the structure and composition of the final products can be regulated through the cation-exchange reaction in the presence of ascorbic acid. In the Co Se-CdSe/MoSe /CdSe cages, MoSe nanosheets function as a bridge to integrate Co Se-CdSe and CdSe on both sides of the MoSe nanosheet shell into a sandwich-like heterostructured catalyst system, which possesses multiple positive merits for photocatalysis, including accelerated transport and separation of photogenerated carriers, improved visible light utilization, and increased catalytic active sites. Thus, the optimized Co Se-CdSe/MoSe /CdSe cages exhibit remarkable visible-light photocatalytic performance and outstanding stability for CO reduction with a high CO average yield of 15.04 µmol g h and 90.14% selectivity, which are much higher than those of other control samples including single-component catalysts and binary hybrid catalysts. This study provides a promising way for the design and fabrication of high-efficiency photocatalysts.
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http://dx.doi.org/10.1002/smll.202100412DOI Listing
August 2021

Inhibitory effects of circulating natural autoantibodies to CD47-derived peptides on OSCC cells.

Oral Dis 2021 May 24. Epub 2021 May 24.

Beijing Institute of Dental Research, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.

Objectives: Natural autoantibodies serve as an important anti-tumorigenic component in the body. This study was thus designed to investigate whether circulating natural IgG autoantibodies against a cluster of differentiation 47 (CD47) could exert inhibitory effects on oral squamous cell carcinoma (OSCC).

Subjects And Methods: The expression levels of 13 tumor-targeted genes in three OSCC cell lines were analyzed by qPCR, and CD47 expression in OSCC tissues was also verified with IHC staining. An in-house ELISA was performed to analyze circulating anti-CD47 IgG levels in control subjects, oral benign tumor, and OSCC patients, and to detect anti-CD47 IgG-abundant plasma. Three OSCC cell lines were treated with anti-CD47 IgG-abundant and -deficient plasma, respectively, followed by the analysis of cell proliferation, apoptosis, and invasion/metastasis.

Results: The CD47 gene showed the highest expression among 13 genes detected in three OSCC cell lines; its expression was significantly higher in OSCC tissues than adjacent tissues. Plasma anti-CD47 IgG levels showed the differences between control subjects, oral benign tumor, and OSCC patients. Anti-CD47 IgG-abundant plasma could evidently reduce cell viability via suppressing p-AKT expression and inducing cell apoptosis and inhibit the invasion of all three OSCC cell lines.

Conclusions: Natural autoantibodies against CD47 may be a potential agent for OSCC immunotherapy.
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http://dx.doi.org/10.1111/odi.13922DOI Listing
May 2021
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