Publications by authors named "Xinyue Zhu"

32 Publications

Establishment of a Multiplex RT-PCR Method for the Detection of Five Known Genotypes of Porcine Astroviruses.

Front Vet Sci 2021 15;8:684279. Epub 2021 Jun 15.

College of Animal Science and Technology, Guangxi University, Nanning, China.

Porcine astroviruses (PAstVs) are prevalent in pigs worldwide, and five genotypes have been reported to circulate in China. However, little is known about the coinfection status of PAstVs. For differential and simultaneous diagnoses of these five genotypes of PAstVs, a multiplex RT-PCR method was established on the basis of the gene of type 1 PAstV, and the genes of type two to five PAstVs. This quintuple PCR system was developed through optimization of multiplex PCR and detection sensitivity and specificity. The results showed that this multiplex RT-PCR method could specifically detect all the five PAstV genotypes without cross-reaction to any other major viruses circulating in Chinese pig farms. The detection limit of this method was as low as 10 pg of standard plasmids of each PAstV genotype. In addition, a total of 275 fecal samples collected from different districts of Guangxi, China, between April 2019 and November 2020, were tested by this newly established multiplex RT-PCR. Moreover, the sensitivity and specificity of monoplex and multiplex RT-PCR methods were compared by detecting the same set of clinical positive samples. The results revealed that PAstV1 (31/275), PAstV2 (49/275), PAstV3 (36/275), PAstV4 (41/275), and PAstV5 (22/275) were all detected, and dual (PAstV1+PAstV2, PAstV1+PAstV3, PAstV2+PAstV3, PAstV2+PAstV4, PAstV3+PAstV4, and PAstV4+PAstV5) or triple genotypes (PAstV1+PAstV2+PAstV3 and PAstV2+PAstV3+PAstV4) of coinfections were also unveiled in this study. The detection result of multiplex PCR was consistent with that of monoplex PCR. Compared with monoplex PCR, this multiplex PCR method showed obvious advantages such as time and cost efficiency and high sensitivity and specificity. This multiplex RT-PCR method offered a valuable tool for the rapid and accurate detection of PAstV genotypes circulating in pig herds and will facilitate the surveillance of PAstV coinfection status.
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http://dx.doi.org/10.3389/fvets.2021.684279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239161PMC
June 2021

Exercise attenuates angiotensinⅡ-induced muscle atrophy by targeting PPARγ/miR-29b.

J Sport Health Sci 2021 Jun 8. Epub 2021 Jun 8.

Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School of Life Science, Shanghai University, Shanghai 200444, China; Shanghai Engineering Research Center of Organ Repair, School of Medicine, Shanghai University, Shanghai 200444, China. Electronic address:

Background: Exercise is beneficial for muscle atrophy. Peroxisome proliferator-activated receptor gamma (PPARγ) and microRNA-29b (miR-29b) have been reported to be responsible for angiotensin Ⅱ (AngⅡ)-induced muscle atrophy. However, it is unclear whether exercise can protect AngⅡ-induced muscle atrophy by targeting PPARγ/miR-29b.

Methods: Skeletal muscle atrophy in both the control group and the run group was established by AngⅡ infusion; after 1 week of exercise training, the mice were sacrificed, and muscle weight was determined. Myofiber size was measured by hematoxylin-eosin and wheat-germ agglutinin staining. Apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling staining. The expression level of muscle atrogenes, including F-box only protein 32 (FBXO32, also called Atrogin-1) and muscle-specific RING-finger 1 (MuRF-1), the phosphorylation level of protein kinase B (PKB, also called AKT)/forkhead box O3A (FOXO3A)/mammalian target of rapamycin (mtorTOR) pathway proteins, the expression level of PPARγ and apoptosis-related proteins, including B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X (Bax), cysteine-aspartic acid protease 3 (caspase-3), and cleaved-caspase-3, were determined by western blot. The expression level of miR-29b was checked by reverse-transcription quantitative polymerase chain reaction. A PPARγ inhibitor (T0070907) or miR-29b overexpression adeno-associated virus serotype-8 (AAV8) was used to demonstrate whether PPARγ activation or miR-29b inhibition mediates the beneficial effects of exercise in AngⅡ-induced muscle atrophy.

Results: Exercise can significantly attenuate AngⅡ-induced muscle atrophy, which is demonstrated by increased skeletal muscle weight, cross-sectional area of myofiber, and activation of AKT/mTOR signaling and by decreased atrogenes expressions and apoptosis. In AngⅡ-induced muscle atrophy mice models, PPARγ was elevated whereas miR-29b was decreased by exercise. The protective effects of exercise in AngⅡ-induced muscle atrophy were inhibited by a PPARγ inhibitor (T0070907) or miR-29b overexpression AAV8.

Conclusion: Exercise attenuates AngⅡ-induced muscle atrophy by activation of PPARγ and suppression of miR-29b.
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http://dx.doi.org/10.1016/j.jshs.2021.06.002DOI Listing
June 2021

Purification and structure analysis of zinc-binding protein from Mizuhopecten yessoensis.

J Food Biochem 2021 06 16;45(6):e13756. Epub 2021 May 16.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, P.R. China.

Zn-binding protein was obtained after purification from scallops (Mizuhopecten yessoensis) using gel permeation and ion-exchange chromatography. Amino acid determination showed that the cysteine of the zinc-binding protein accounted for one-third of the total amino acids, which is a typical feature of metallothionein (MT). The spectra of Fourier Transform Infrared Spectroscopy (FTIR) and Circular Dichroism (CD) were analyzed to predict the secondary structure information of zinc-binding protein: the α-helix was 46.55%, the β-sheets was 27.07%, the random coil was 16.48%, and the β-turns was 9.89%. Using a commercial kit to measure its antioxidant activity in vitro, the result showed that it had good scavenging ability to 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (·OH), and reducing the ability to ferrous iron ions. With the process provided by this study, zinc-binding protein can be prepared in large quantities, which is the basis for its future commercialization. PRACTICAL APPLICATIONS: According to the extraction and purification process established in this study, a large amount of zinc-bound MT from the viscera of scallops can be obtained. And the zinc-bound MT had good antioxidant activity. In addition, the yield of each purification step has been calculated. The zinc-bound MTs from scallop' viscera can be prepared in large quantities by directly using the process in this manuscript or by equal magnification of this process. In the future, large-scale production can be considered to increase the economic value of scallops' viscera.
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http://dx.doi.org/10.1111/jfbc.13756DOI Listing
June 2021

2D Honeycomb-Kagome Polymer Tandem as Effective Metal-Free Photocatalysts for Water Splitting.

Adv Mater 2021 May 4;33(21):e2008645. Epub 2021 May 4.

TU Dresden Fakultät für Chemie und Lebensmittelchemie, Bergstraße 66c, 01062, Dresden, Germany.

On the basis of first-principles calculations, the potential of applying 2D honeycomb-kagome polymers made of heteroatom-centered triangulene derivatives to photocatalyze water splitting is explored. The designed 2D polymers possess indirect bandgaps in the range of 1.80-2.84 eV and show pronounced light absorption in the ultraviolet and visible region of the solar spectrum. With suitable band edge alignment, the examined N- and B-center polymers can generate sufficient photon-excited electrons and holes to activate the hydrogen and oxygen evolution reactions, respectively. The combination of lattice-inherent band features (flat bands) with chemical functionalization (potential shift due to heteroatoms) makes it possible to construct tandem cells with suppressed electron/hole recombination for effective overall water splitting. In addition, there is a potential difference between the half-electrodes that can be utlized to power​ auxiliary components in self-sufficient photocatalyzers.
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http://dx.doi.org/10.1002/adma.202008645DOI Listing
May 2021

Delivery of CW laser power up to 300 watts at 1080 nm by an uncooled low-loss anti-resonant hollow-core fiber.

Opt Express 2021 Jan;29(2):1492-1501

In this paper, we report the use of a 3-meter low-loss anti-resonant hollow-core fiber (AR-HCF) to deliver up to 300 W continuous-wave laser power at 1080 nm wavelength from a commercial fiber laser source. A near-diffraction-limited beam is measured at the output of the AR-HCF and no damage to the uncooled AR-HCF is observed for several hours of laser delivery operation. The limit of AR-HCF coupling efficiency and laser-induced thermal effects that were observed in our experiment are also discussed.
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http://dx.doi.org/10.1364/OE.415494DOI Listing
January 2021

Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata.

Protein Expr Purif 2021 Jun 6;182:105838. Epub 2021 Feb 6.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.

Zinc-binding proteins named MT-M-I and MT-M-II were obtained after purification from metal-exposed hairy clams (Arca subcrenata) using gel permeation and ion-exchange chromatography. MT-M-I and MT-M-II were resolved by ion-exchange chromatography, and they were found to have similar molecular weights. MT-M-I and MT-M-II can bind 6 and 7 equivalents of Zn in vitro, and they showed unusual migration behaviors in Tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis (Tricine-SDS-PAGE). Such migration behaviors may be due to themetal thiolate clusters in these proteins. In terms of amino acid composition, the proportion of cysteine in MT-M-I and MT-M-II was approximately 30%, and glycine accounted for approximately 15%, where as aromatic amino acids were absent. Considering the performance in Tricine-SDS-PAGE and the amino acid compositions, MT-M-I and MT-M-II conform to the molecular characteristics of the metallothionein proteins. The structures were explored using circular dichroism (CD) and Fourier-transform infrared spectroscopy (FTIR). Also determined the antioxidant activities in terms of DPPH radical scavenging ability, hydroxyl radical (·OH) scavenging ability, and ferric-reducing/antioxidant power. The antioxidant activities of MT-M-I were found to be stronger than those of MT-M-II.
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http://dx.doi.org/10.1016/j.pep.2021.105838DOI Listing
June 2021

CT-Guided Percutaneous Lumbar Ligamentum Flavum Release by Needle Knife for Treatment of Lumbar Spinal Stenosis: A Case Report and Literature Review.

J Pain Res 2020 13;13:2073-2081. Epub 2020 Aug 13.

Department of Acupuncture and Moxibustion, China-Japan Friendship Hospital, Beijing, People's Republic of China.

Case: The patient was a 62-year-old woman presenting with low-back pain of 3 years' duration and numbness in the right leg.

Diagnosis: She was diagnosed with lumbar spinal stenosis based on combined magnetic resonance imaging, physical examination, and symptoms.

Interventions: Treatment with computed tomography-guided percutaneous release of the ligamentum flavum was delivered.

Outcomes: Relief of symptoms immediately after treatment and complete resolution of symptoms after 1 month were achieved. At follow-up, there was no recurrence of symptoms after 2, 4, 6, and 12 months.

Conclusion: Lumbar ligamentum flavum hypertrophy is an important cause of degenerative lumbar spinal stenosis. The hypertrophic ligamentum flavum can directly compress the spinal canal, squeeze the cauda equina, and simultaneously lead to a reduction in the anteroposterior diameter of the intervertebral foramen, which compresses the nerve roots and causes numbness and other symptoms of lower extremities. In clinical practice, doctors should combine imaging findings with patient symptoms for diagnosis and an individualized treatment plan for each patient with lumbar spinal stenosis, and conduct gradual stepwise treatment using conservative minimally invasive surgery to prevent excessive surgery.
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http://dx.doi.org/10.2147/JPR.S255249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434627PMC
August 2020

Ultrasound-guided acupuncture of the sphenopalatine ganglion for the treatment of vasomotor rhinitis: a case report.

Acupunct Med 2020 10 1;38(5):361-363. Epub 2020 Apr 1.

Department of Acupuncture-Moxibustion, China-Japan Friendship Hospital, Beijing, China.

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http://dx.doi.org/10.1177/0964528420906414DOI Listing
October 2020

Ultrasound-Guided Percutaneous Release Procedures in the Lumbar Ligamentum Flavum by Acupotomy: A Cadaveric study.

Evid Based Complement Alternat Med 2019 23;2019:2807901. Epub 2019 Nov 23.

Health Science Center, Peking University, No. 38 Xueyuan Road, Beijing 100191, China.

Objective: This study aims to determine the methods of percutaneous release procedures in the lumbar ligamentum flavum (LF) under ultrasound guidance by acupotomy and provide an anatomical basis for intrusive treatment of lumbar disc herniation and lumbar spinal canal stenosis.

Methods: Twelve cadavers including 4 females and 8 males aged 60 to 90 years (73.42 ± 14.57 years), without formalin fixation, were selected. Guided by an ultrasound transducer, we punctured acupotomy to release lumbar LF in L3/L4, L4/L5, and L5/S1 segments. In the transverse-axis approach, the probe was placed transversely, while in the longitudinal-axis approach, the probe was placed longitudinally. The depth of needle penetration (A), the distance between the puncture point and spinous process (B), and the distance between the puncture point and sacral cornu (C) were measured on cadavers, and the depth of needle penetration (U-A), the distance between the puncture point and spinous process (U-B), and the angle for acupotomy (D) on ultrasound images were also measured. Statistical analyses were carried out using SPSS. Paired sample -tests and homogeneity of variance tests and one-way analysis of variance (ANOVA) were performed. The Pearson correlation coefficients and linear correlation coefficients were calculated for the data obtained from ultrasound and cadaver measurements.

Results: No obvious blood vessels and nerves were observed in the puncture path, and the spinal dura was intact. There was no statistical difference between the left and right side measurements obtained from the ultrasound images and the cadavers. The penetration depth in the transverse-axis approach was less than that in the longitudinal-axis approach, and the angle of the needle in the transverse-axis approach was greater than that in the longitudinal-axis approach. The measured data for the transverse-axis approach for L3/L4, L4/L5, and L5/S1 segments showed that there were no differences in the needle angle, the depth of needle penetration, and the distance from the spinous process to the puncture point among the three segments. There was a strong correlation between the depth of needle penetration and the distance from the spinous process to the puncture point on the ultrasonic images and the cadavers on the path of acupotomy. Linear equation A = 2.02 + 0.83  U-A,  = 0.352; B = 1.37 + 0.71  U-B,  = 0.252, where A/B refers to the data measured on the cadavers and U-A/U-B refers to the data measured on the ultrasound images.

Conclusion: In this study, ultrasound guidance was applied, which better guaranteed the safety and feasibility of acupotomy therapy. Before performing the treatment, the depth of needle penetration in the human body can be determined by measuring the distance between the needle point and the target position on the ultrasound image. Under ultrasound guidance, the transverse-axis approach has a smaller puncture depth and greater puncture angle than the longitudinal-axis approach. Hence, this study believes that the transverse-axis approach is safer for the clinical application of ultrasound-guided LF acupotomy lysis.
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http://dx.doi.org/10.1155/2019/2807901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906825PMC
November 2019

Discovery of -Aryl-pyridine-4-ones as Novel Potential Agrochemical Fungicides and Bactericides.

J Agric Food Chem 2019 Dec 5;67(50):13904-13913. Epub 2019 Dec 5.

Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection , Hainan University , Haikou , Hainan 570228 , People's Republic of China.

A series of -aryl-pyridine-4-one derivatives were designed and synthesized using maltol and antidesmone as lead compounds, and then their fungicidal/bactericidal activities and possible mechanism of action against were explored. Most of these compounds exhibited significant fungicidal activity . Especially, compound has more than 90% inhibitory activity against nine plant pathogenic fungi at 50 μg mL, which is superior to azoxystrobin. Moreover, an bioassay also demonstrated that compound exhibited high-efficiency broad-spectrum antifungal activity and can effectively control postharvest diseases of mango. In addition, it was found that compounds and can also effectively control rice bacterial leaf blight in pot experiments, which was even more effective than zhongshengmycin. Preliminary mechanism studies revealed that compound may cause cell membrane and mitochondria destruction. These findings indicate that compound can be used to develop potential agrochemical fungicides and bactericides.
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http://dx.doi.org/10.1021/acs.jafc.9b06296DOI Listing
December 2019

Acupotomy by an ultrasound-guided technique: A protocol for a systematic review.

Medicine (Baltimore) 2019 Oct;98(42):e17398

Department of acupuncture-moxibustion, China-Japan Friendship Hospital, Beijing.

Background: Acupotomy is a miniature surgery instrument. It can cut and detach the abnormal, cicatricial, and contractured tissues by causing only microtrauma. Acupotomy has been widely used clinically with a satisfactory efficacy. With the development of ultrasound technology, ultrasound-guided acupotomy has shown great value in clinical practice. But it is not yet clear that ultrasound-guided acupotomy is very effective and safe. Therefore, it is important to re-evaluate the available evidence to reach a relatively convincing conclusion that acupotomy by ultrasound-guided technique is a better choice than traditional acupotomy. The purpose of this systematic review is to provide a method for evaluating the effectiveness and safety of acupotomy by ultrasound-guided technique.

Methods: This systematic review will be performed by searching relevant randomized controlled trials (RCTs) without any language or publication status restriction from inception to December 2019 by 2 researchers in nine databases (PubMed, Medline, Embase, Cochrane Library, Chinese literature databases, Chinese Biomedical Literature Database [CBM], China National Knowledge Infrastructure [CNKI], China Science and Journal Database [CSJD], and Wanfang Database). All RCTs evaluating acupotomy by the ultrasound-guided technique will be included in this study. Visual analog scale (VAS) and change of symptom will be assessed as the primary outcomes. The change in the ultrasound image, safety and adverse events, and acceptability will be assessed as secondary outcomes. The selection of study, data collection and analysis, and assessment of the study quality will be completed independently by 2 researchers. RevMan v.5.3 will be used for meta-analysis if no significant heterogeneity is detected. Continuous outcomes will be presented as the mean difference (MD) or standardized MD, while dichotomous data will be expressed as the relative risk.

Results: This study will provide a high-quality synthesis of QL and AR to assess the effectiveness and safety of acupotomy by ultrasound-guided technique.

Conclusion: This systematic review will provide evidence to judge whether acupotomy by ultrasound-guided technique is an effective the efficacy and safety intervention.

Prospero Registration Number: CRD42018109070.
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http://dx.doi.org/10.1097/MD.0000000000017398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824636PMC
October 2019

Dehydrozingerone Inspired Discovery of Potential Broad-Spectrum Fungicidal Agents as Ergosterol Biosynthesis Inhibitors.

J Agric Food Chem 2019 Oct 2;67(41):11354-11363. Epub 2019 Oct 2.

Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection , Hainan University , Haikou 570228 , People's Republic of China.

A series of dehydrozingerone derivatives were synthesized, and their fungicidal activities and action mechanism against were evaluated. The bioassay result showed that most compounds exhibited excellent fungicidal activity at 50 μg mL. Compounds , , , , and exhibited broad-spectrum fungicidal activity; especially, compounds and were found to have more potent fungicidal activity than azoxystrobin. The EC values of compounds and against were 0.943 and 0.161 μg mL respectively. Moreover, compound exhibited significant bactericidal activity against pv, with an EC value of 11.386 μg mL, and its curative effect (49.64%) and protection effect (51.74%) on rice bacterial blight disease was equivalent to that of zhongshengmycin (42.90%, 40.80% respectively). Compound could also effectively control gray mold (87.10%, 200 μg mL) and rice sheath blight (100%, 200 μg mL; 82.89%, 100 μg mL) . Preliminary action mechanism study showed that compound mainly acted on the cell membrane and significantly inhibited ergosterol biosynthesis in .
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http://dx.doi.org/10.1021/acs.jafc.9b04231DOI Listing
October 2019

Lysosome-targeted two-photon fluorescent probe for detection of hypobromous acid in vitro and in vivo.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Apr 17;212:48-54. Epub 2018 Dec 17.

College of Chemistry and Chemical Engineering, Lanzhou University, China. Electronic address:

It is found that hypobromous acid (HOBr) can affect the activity of type IV collagen. Herein, we synthesized a lysosome-targeted fluorescence probe NA-lyso based on Suzuki coupling reaction with naphthalimide as a fluorescent group. HOBr can oxidize the amino group and methylthio group, which increased the degree of conjugation of the probe, thereby affecting its optical properties. Accordingly, it can establish a method for the specific detection of HOBr. NA-lyso has the properties including fast response, high fluorescence quantum yield (Φ = 59.17%), high selectivity, low cytotoxicity and good membrane-permeability. The probe can locate to lysosome of cells. The potential of the probe as biosensor for HOBr was demonstrated by imaging of exogenous and endogenous HOBr in living cells and in mice. In consequence, NA-lyso is expected to be a powerful tool to detect HOBr in complex biosystem and provides a means of exploring physiological functions associated with HOBr in living organisms.
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http://dx.doi.org/10.1016/j.saa.2018.12.029DOI Listing
April 2019

Vertigo caused by longus colli tendonitis: A case report and literature review.

Medicine (Baltimore) 2018 Nov;97(45):e13130

Department of acupuncture-moxibustion, China-Japan Friendship Hospital.

Rationale: Tendinitis of the longus colli muscle is an aseptic inflammatory process leading to acute posterior neck pain, neck stiffness, and dysphagia or odynophagia. We present a patient exhibiting an infrequent symptom, vertigo. This is the first description of the occurrence of vertigo symptoms caused by longus colli tendinitis.

Patient Concerns: A 38-year-old man was diagnosed with vertigo, presenting with a 1-month history of dizziness, palpitations, and numbness in the hands.

Diagnosis: Longus colli tendinitis. The diagnosis was established using magnetic resonance imaging fat-suppression sequences.

Interventions: Treatment with corticosteroid injections and acupotomy.

Outcomes: The symptoms relieved immediately after the treatment and complete resolution of the symptoms was observed after 1 week.

Lessons: Longus colli tendinitis with vertigo is an under-reported condition in the literature and physicians should be aware of its existence. A lack of familiarity with the anatomy of the prevertebral space and its variable radiographic appearance makes the diagnosis of longus colli tendinitis clinically difficult. Misdiagnosis of this condition may lead to unnecessary interventions in vertigo.
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http://dx.doi.org/10.1097/MD.0000000000013130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250553PMC
November 2018

Synthesis and application of ratio fluorescence probe for chloride.

Anal Bioanal Chem 2018 Oct 20;410(25):6507-6516. Epub 2018 Jul 20.

College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, Gansu, China.

As chloride ions (Cl) play a vital role in maintaining normal physiological activity, detection of chloride ions is quite urgent. Hence, we developed chloride fluorescence probes to highly selectively and sensitively monitor chloride ions. The probe M2 with single emission has a high fluorescence quantum yield (Φ = 45%), and it is capable of quantitative detection of Cl under physiological conditions (pH = 7.4) and pH = 5.0 with a linear range of 0.1-4.0 mM; nevertheless, it is of the switch-off type. We further synthesized a ratiometric fluorescent probe MY with M2 as raw material, which featured excellent selectivity and anti-interference, and large two-photon cross section (555 GM). The probe is conveniently used to detect Cl in water samples and biological samples including human sweat, serum, and urine samples, indicating it holds great promise for chloride detection and biological application. Graphical abstract ᅟ.
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http://dx.doi.org/10.1007/s00216-018-1250-0DOI Listing
October 2018

Sensitive naked eye detection and quantification assay for nitrite by a fluorescence probe in various water resources.

Spectrochim Acta A Mol Biomol Spectrosc 2018 Jul 21;200:275-280. Epub 2018 Apr 21.

College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China; Zhongwei High-tech Institute of Lanzhou University, Zhongwei 755000, China. Electronic address:

An uncontrolled increase of nitrite concentration in groundwater, rivers and lakes is a growing threat to public health and environment. It is important to monitor the nitrite levels in water and clinical diagnosis. Herein, we developed a switch-off fluorescence probe (PyI) for the sensitive detection of nitrite ions in the aqueous media. This probe selectively recognizes nitrite ions through a distinct visual color change from colorless to pink with a detection limit of 0.1 μM. This method has been successfully applied to the determination of nitrites in tap water, lake water and Yellow River water with recoveries in the range of 94.8%-105.4%.
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http://dx.doi.org/10.1016/j.saa.2018.04.025DOI Listing
July 2018

Highly Selective Fluorescent Probe Based on Hydroxylation of Phenylboronic Acid Pinacol Ester for Detection of Tyrosinase in Cells.

Anal Chem 2018 01 15;90(1):855-858. Epub 2017 Dec 15.

State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, China.

The detection of tyrosinase, a biomarker for melanoma, is of great significance. Herein, a fluorescent tyrosinase probe, with resorufin as the fluorophore and m-tolylboronic acid pinacol ester as the receptor, is proposed. The response relies on the tyrosinase-catalyzed hydroxylation of phenylboronic acid pinacol ester at an adjacent position followed by 1,6-rearrangement elimination to release resorufin. This probe well quantifies tyrosinase in the range from 1 to 100 U mL with a detection limit of 0.5 U mL. Importantly, the probe exhibits high selectivity for tyrosinase over other biological substances including reactive oxygen species. In addition, it is successfully applied to the imaging of tyrosinase in cells. This probe provides a novel platform for selective detection of tyrosinase in biosystems.
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http://dx.doi.org/10.1021/acs.analchem.7b03681DOI Listing
January 2018

A ratiometric mitochondria-targeting two-photon fluorescent probe for imaging of nitric oxide in vivo.

Analyst 2017 Dec;142(24):4623-4628

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

A two-photon ratiometric fluorescent probe (Mito-N) has been developed for monitoring mitochondrial nitric oxide (NO) in vivo. Mito-N exhibited excellent selectivity and sensitivity toward NO among various ROS/RNS. The successful two-photon imaging of mitochondrial NO proved that Mito-N is a powerful noninvasive imaging tool in living systems.
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http://dx.doi.org/10.1039/c7an01461aDOI Listing
December 2017

Extraction of Illegal Dyes from Red Chili Peppers with Cholinium-Based Deep Eutectic Solvents.

J Anal Methods Chem 2017 31;2017:2753752. Epub 2017 Jul 31.

Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

Deep eutectic solvents (DESs) as a new kind of green solvents have been used to extract bioactive compounds but there are few applications in extracting chrysoidine dyes. In this study, we developed an ultrasonic-assisted extraction method with choline chloride/hydrogen bond donor (ChCl/HBD) DES for the extraction of chrysoidine G (COG), astrazon orange G (AOG), and astrazon orange R (AOR) in food samples. Some experimental parameters, such as extraction time, raw material/solvent ratio, and temperature, were evaluated and optimized as follows: the ratio of ChCl/HBD, 1 : 2 (v/v); the ratio of sample/DES, 1 : 10 (g/mL); extraction time, 20 min; extraction temperature, 50°C. Under the optimized conditions, the limits of detection (g/mL) were 0.10 for COG and 0.06 for AOG and AOR. The relative standard deviations were in the range of 1.2-2.1%. The recoveries of the three dyes were in the range of 80.2-105.0%. By comparing with other commonly used solvents for extracting chrysoidine dyes, the advantages of DESs proved them to be potential extraction solvents for chrysoidine G, astrazon orange G, and astrazon orange R in foods.
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http://dx.doi.org/10.1155/2017/2753752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555004PMC
July 2017

MiR-613 suppresses retinoblastoma cell proliferation, invasion, and tumor formation by targeting E2F5.

Tumour Biol 2017 Mar;39(3):1010428317691674

1 Department of Ophthalmology, Medical School of Nanjing University, Jinling Hospital, Nanjing, China.

Retinoblastoma is a common intraocular malignancy that occurs during childhood. MicroRNAs play critical roles in the regulation of retinoblastoma initiation and progression, and aberrant expression of miR-613 had been reported in various types of cancer. However, the role and mechanism of its function in retinoblastoma are still unclear. In this study, we found that miR-613 was downregulated in retinoblastoma tissues and cell lines. Overexpression of miR-613 suppressed retinoblastoma cell proliferation, migration, and invasion and induced cell cycle arrest in vitro. Additionally, overexpressed miR-613 also inhibited tumor formation of retinoblastoma cells in vivo. We further identified E2F5 as a direct target of miR-613. Reintroduction of E2F5 without 3'-untranslated region reversed the inhibitory effects of miR-613 on cell proliferation and invasion. Our data collectively indicate that miR-613 functions as a tumor suppressor in retinoblastoma through downregulating E2F5, supporting the targeting of the novel miR-613/E2F5 axis as a potentially effective therapeutic approach for retinoblastoma.
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http://dx.doi.org/10.1177/1010428317691674DOI Listing
March 2017

A simple ratiometric fluorescent sensor for fructose based on complexation of 10-hydroxybenzo[h]quinoline with boronic acid.

Spectrochim Acta A Mol Biomol Spectrosc 2017 Jun 7;180:199-203. Epub 2017 Mar 7.

State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. Electronic address:

A simple ratiometric fluorescent sensor for fructose was presented. It consisted of 10-hydroxybenzo[h]quinoline (HBQ) which showed emission at 572nm and 3-pyridylboronic acid (PDBA) whose complex with HBQ gave emission at 500nm. The reaction of fructose with PDBA inhibited the complexation of HBQ with PDBA, resulting in the change of dual-emission intensity ratio. The sensor well quantified fructose in the range of 0.015-2.5mM with detection limit of 0.005mM. Besides, this sensor exhibited excellent selectivity and was successfully applied to fructose detection in food. This work provides a simple ratiometric sensing platform for sensitive and selective detection of fructose.
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http://dx.doi.org/10.1016/j.saa.2017.03.017DOI Listing
June 2017

A near-infrared fluorescent probe based on chloroacetate modified naphthofluorescein for selectively detecting cysteine/homocysteine and its application in living cells.

Photochem Photobiol Sci 2016 Nov;15(11):1393-1399

Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.

We have prepared a near-infrared (NIR) turn-on fluorescent probe (NFC) based on chloroacetate modified naphthofluorescein for specific detection of cysteine (Cys) and homocysteine (Hcy) over glutathione (GSH) and other amino acids (AAs) with the detection limits of 0.30 μM and 0.42 μM, respectively. The fluorescence intensity of the naphthofluorescein (NF) chromophore is modulated by an internal charge transfer (ICT) process. The probe NFC is readily available and weakly fluorescent, but of observably enhanced fluorescence after reacting with Cys or Hcy. We assumed and then demonstrated that the fluorescence off-on process involves a conjugate nucleophilic substitution/cyclization sequence. Furthermore, the probe has been successfully applied for detecting the total content of Cys and Hcy in human plasma and imaging in living cells with low toxicity.
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http://dx.doi.org/10.1039/c6pp00219fDOI Listing
November 2016

Non-targeted and targeted metabolomics approaches to diagnosing lung cancer and predicting patient prognosis.

Oncotarget 2016 Sep;7(39):63437-63448

The Affiliated Luohu Hospital of Shenzhen University, Shenzhen 518001, China.

Lung cancer is the most common cause of cancer death in China. We characterized metabolic alterations in lung cancer using two analytical platforms: a non-targeted metabolic profiling strategy based on proton nuclear magnetic resonance (1H-NMR) spectroscopy and a targeted metabolic profiling strategy based on rapid resolution liquid chromatography (RRLC). Changes in serum metabolite levels during oncogenesis were evaluated in 25 stage I lung cancer patients and matched healthy controls. We identified 25 metabolites that were differentially regulated between the lung cancer patients and matched controls. Of those, 16 were detected using the non-targeted approach and 9 were identified using the targeted approach. Both groups of metabolites could differentiate between lung cancer patients and healthy controls with 100% sensitivity and specificity. The principal metabolic alternations in lung cancer included changes in glycolysis, lipid metabolism, choline phospholipid metabolism, one-carbon metabolism, and amino acid metabolism. The targeted metabolomics approach was more sensitive, accurate, and specific than the non-targeted metabolomics approach. However, our data suggest that both metabolomics strategies could be used to detect early-stage lung cancer and predict patient prognosis.
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http://dx.doi.org/10.18632/oncotarget.11521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325375PMC
September 2016

A new fluorescence turn-on probe for biothiols based on photoinduced electron transfer and its application in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2016 09 5;166:31-37. Epub 2016 May 5.

Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic address:

A new biothiol-selective fluorescent probe 1 based on photoinduced electron transfer (PET) mechanism was designed and synthesized. The UV-Vis absorption and fluorescent emission properties of probe 1 towards various analytes were studied in detail. The probe exhibited a large stokes shift (~200nm) after reacted with biothiols and could selectively detect cysteine (Cys) in dimethyl sulfoxide (DMSO)/H2O solution (9:1, v/v, 10mM phosphate buffer saline, pH3.5) over glutathione (GSH), homocysteine (Hcy) and other analytes with a detection limit of 0.117μM. In addition, probe 1 responded well to GSH, Hcy and Cys in the same above solution with pH5.5 and got the detection limits of 0.151μM, 0.128μM and 0.037μM, respectively. Probe 1 was of very low cytotoxicity and successfully applied for imaging of thiols in living cells.
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http://dx.doi.org/10.1016/j.saa.2016.05.004DOI Listing
September 2016

Naphthalimide derived fluorescent probes with turn-on response for Au(3+) and the application for biological visualization.

Biosens Bioelectron 2016 Sep 13;83:334-8. Epub 2016 Apr 13.

State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. Electronic address:

The 4-N,N-dimethyl-1,8-naphthalimide based fluorescent probes have been explored for selective detection of Au(3+). Both probes show a pronounced fluorescence enhancement response to Au(3+). Hydroxy is introduced as ligand of Au(3+) for Probe 1 and the newly designed Probe 2 contains an alkyne moiety to recognize Au(3+) through an irreversible C≡C bond hydrolysis reaction. Probe 1 exhibits higher performance such as faster response, lower detection limit of 0.050μM and the better responsive effect in 99.5% water system compared with most of probes published. The Probe 2 displays high stability to pH, suitable water solubility, wider linear range (0-100μM) to Au(3+), and live-cells imaging with low cytotoxicity.
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http://dx.doi.org/10.1016/j.bios.2016.04.034DOI Listing
September 2016

High-efficiency extraction of nucleosides based on the combination of self-assembly ionic liquid layer and boronic acid-functionalized attapulgite.

Talanta 2016 06 27;153:71-8. Epub 2016 Feb 27.

State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. Electronic address:

Boronate affinity materials have been widely used for selective capture of cis-diol-containing molecules, but most do not have sufficient extraction efficiency. We have prepared boronic acid-functionalized attapulgite, and then it was coated with imidazolium-based ionic liquid, 1-dodecyl-3-methylimidazolium bromide (C12mimBr), via physical self-assembly process. The extraction efficiency of the material increased dramatically after coated with C12mimBr, and its enrichment ratios for nucleosides increased by 9- to 282-fold. Besides, the C12mimBr-coated adsorbent did not lose selectivity and was able to capture cis-diols in the presence of a 100-fold excess of interferences. C12mimBr-coated material was applied to selective enrichment of nucleosides from human urine. The limits of detection and the limits of quantification were in the range of 0.06-0.46ngmL(-1) and 0.20-1.53ngmL(-1), respectively. Reproducibility of the method was determined with relative standard deviations≤9.9%. The recoveries of the target nucleosides from spiked human urine were in the range of 87.8-109.6%. In our preception, self-assembly ionic liquid layer can serve as a promising alternative to improve the extraction efficiency of boronate affinity materials.
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http://dx.doi.org/10.1016/j.talanta.2016.02.004DOI Listing
June 2016

Suppression of microRNA-125a-5p upregulates the TAZ-EGFR signaling pathway and promotes retinoblastoma proliferation.

Cell Signal 2016 08 14;28(8):850-60. Epub 2016 Apr 14.

Medical School of Nanjing University, Department of Ophthalmology, Jinling Hospital, Nanjing 210002, China; Department of Ophthalmology, Jinling Hospital, Nanjing 210002, China. Electronic address:

Retinoblastoma is the most common intraocular malignancy that occurs during childhood; however, the mechanism underlying retinoblastoma proliferation and progression remains unclear. MicroRNAs (miRNAs) play an important role in the regulation of a myriad of biological processes in various types of cancer. In this study, we performed microarray analysis followed by qRT-PCR using four classes of retinoblastoma tissues with increasing cTNM classification stages to identify crucial miRNAs whose expression was correlated with retinoblastoma progression. miR-125a-5p was downregulated, and its expression levels were inversely correlated with cell proliferation in retinoblastoma compared with adjacent non-tumor retinal tissues. The overexpression of miR-125a-5p significantly suppressed cell proliferation and tumor formation in retinoblastoma. We further identified the transcriptional co-activator with PDZ binding motif (TAZ) as a direct target of miR-125a-5p. Importantly, TAZ levels were inversely correlated with miRNA-125a-5p expression, and TAZ promoted retinoblastoma cell proliferation. Moreover, the overexpression of miR-125a-5p led to a decrease in TAZ expression and downstream EGFR signaling pathway activation both in vitro and vivo. Finally, TAZ overexpression in retinoblastoma cells overexpressing miR-125a-5p restored retinoblastoma cell proliferation and EGFR pathway activation. Taken together, our data demonstrated that miR-125a-5p functions as an important tumor suppressor that suppresses the EGFR pathway by targeting TAZ to inhibit tumor progression in retinoblastoma. Thus, the miR-125a-5p/TAZ/EGFR axis may be a potential therapeutic target for retinoblastoma.
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http://dx.doi.org/10.1016/j.cellsig.2016.04.002DOI Listing
August 2016

Downregulation of HDAC9 inhibits cell proliferation and tumor formation by inducing cell cycle arrest in retinoblastoma.

Biochem Biophys Res Commun 2016 Apr 28;473(2):600-6. Epub 2016 Mar 28.

Medical School of Nanjing University, Department of Ophthalmology, Jinling Hospital, Nanjing, 210002, China. Electronic address:

Histone deacetylase 9 (HDAC9) is a member of class II HDACs, which regulates a wide variety of normal and abnormal physiological functions. Recently, HDAC9 has been found to be overexpressed in some types of human cancers. However, the role of HDAC9 in retinoblastoma remains unclear. In this study, we found that HDAC9 was commonly expressed in retinoblastoma tissues and HDAC9 was overexpressed in prognostically poor retinoblastoma patients. Through knocking down HDAC9 in Y79 and WERI-Rb-1 cells, the expression level of HDAC9 was found to be positively related to cell proliferation in vitro. Further investigation indicated that knockdown HDAC9 could significantly induce cell cycle arrest at G1 phase in retinoblastoma cells. Western blot assay showed downregulation of HDAC9 could significantly decrease cyclin E2 and CDK2 expression. Lastly, xenograft study in nude mice showed that downregulation of HDAC9 inhibited tumor growth and development in vivo. Therefore, our results suggest that HDAC9 could serve as a novel potential therapeutic target in the treatment of retinoblastoma.
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http://dx.doi.org/10.1016/j.bbrc.2016.03.129DOI Listing
April 2016

A two-photon off-on fluorescence probe for imaging thiols in live cells and tissues.

Photochem Photobiol Sci 2016 Mar 23;15(3):412-9. Epub 2016 Feb 23.

Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

A two-photon (TP) fluorescence imaging probe (Z1) was designed to detect biothiols through a photoinduced electron transfer pathway utilizing N-butyl-naphthalimide as the fluorophore and 2,4-dinitrobenzene-sulfonyl as the responsive group, which were linked together by piperazine. The synthesized Z1 displayed high selectivity to biothiols, significant fluorescence off-on properties, and a marked two-photon absorption cross section (δ = 110 GM). Moreover, Z1 showed good biocompatibility and insensitivity toward changes in the biologically relevant pH range (7.2-8.4), which enabled the utilization of Z1 to monitor biothiol levels not only in live cells but also in tissues at depths of 50-250 μm.
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http://dx.doi.org/10.1039/c5pp00468cDOI Listing
March 2016

Metabolic characterization of asthenozoospermia using nontargeted seminal plasma metabolomics.

Clin Chim Acta 2015 Oct 2;450:254-61. Epub 2015 Sep 2.

Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China. Electronic address:

Background: Asthenozoospermia (AS) is a common cause of male infertility. Due to the limitation of routine semen analysis, metabolic alterations associated with the disease are unclear. We applied a metabolic profiling strategy as a surrogate method to accurately assess and provide new insights into the etiologies of asthenozoospermia.

Methods: Seminal plasma samples from patients diagnosis with asthenozoospermia (n=33) and healthy subjects (n=30) were investigated using a nontargeted metabolomics approach based on proton nuclear magnetic resonance ((1)H NMR) spectroscopy. The spectral data were then subjected to multivariate and univariate analyses to identify metabolites that were correlated with asthenozoospermia. The disturbed metabolic pathways which the biomarkers were involved in were analyzed.

Results: Nineteen metabolites including up-regulation or down-regulation of several amino acids, changes in lipids metabolism, phospholipids (choline) metabolism, cholesterol metabolism, nucleoside metabolism, the Krebs cycle and energy metabolism were identified and associated with asthenozoospermia. In particular, the elevation of oxysterols such as 5α-cholesterol and 7-ketocholesterol in seminal plasma of patients with asthenozoospermia was an important finding, indicating the important role of oxidative stress in the mechanism of asthenozoospermia.

Conclusions: The excellent performance of this metabolomics approach offer a highly novel means of etiological diagnosis of asthenozoospermia.
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http://dx.doi.org/10.1016/j.cca.2015.09.001DOI Listing
October 2015
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