Publications by authors named "Xinyue Li"

150 Publications

HS-SPME-GC-MS and Electronic Nose Reveal Differences in the Volatile Profiles of Flowers.

Molecules 2021 Sep 6;26(17). Epub 2021 Sep 6.

The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.

Floral fragrance is one of the most important characteristics of ornamental plants and plays a pivotal role in plant lifespan such as pollinator attraction, pest repelling, and protection against abiotic and biotic stresses. However, the precise determination of floral fragrance is limited. In the present study, the floral volatile compounds of six accessions exhibiting from faint to highly fragrant were comparatively analyzed via gas chromatography-mass spectrometry (GC-MS) and Electronic nose (E-nose). A total of 42 volatile compounds were identified through GC-MS analysis, including monoterpenoids (18 compounds), sesquiterpenoids (12), benzenoids/phenylpropanoids (8), fatty acid derivatives (2), and others (2). In 'ZS', . 'Gaoling', . 'Jin', 'Caixia', and 'Zhaoxia', monoterpenoids were abundant, while sesquiterpenoids were found in large quantities in . 'KMH'. Hierarchical clustering analysis (HCA) divided the 42 volatile compounds into four different groups (I, II, III, IV), and Spearman correlation analysis showed these compounds to have different degrees of correlation. The E-nose was able to group the different accessions in the principal component analysis (PCA) corresponding to scent intensity. Furthermore, the pattern-recognition findings confirmed that the E-nose data validated the GC-MS results. The partial least squares (PLS) analysis between floral volatile compounds and sensors suggested that specific sensors were highly sensitive to terpenoids. In short, the E-nose is proficient in discriminating accessions of different volatile profiles in both quantitative and qualitative aspects, offering an accurate and rapid reference technique for future applications.
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http://dx.doi.org/10.3390/molecules26175425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433901PMC
September 2021

Influencing factors of Barthel index scores among the community-dwelling elderly in Hong Kong: a random intercept model.

BMC Geriatr 2021 09 6;21(1):484. Epub 2021 Sep 6.

School of Data Science, City University of Hong Kong, Hong Kong, China.

Background: Barthel Index (BI) is one of the most widely utilized tools for assessing functional independence in activities of daily living. Most existing BI studies used populations with specific diseases (e.g., Alzheimer's and stroke) to test prognostic factors of BI scores; however, the generalization of these findings was limited when the target populations varied.

Objectives: The aim of the present study was to utilize electronic health records (EHRs) and data mining techniques to develop a generic procedure for identifying prognostic factors that influence BI score changes among community-dwelling elderly.

Methods: Longitudinal data were collected from 113 older adults (81 females; mean age = 84 years, SD = 6.9 years) in Hong Kong elderly care centers. Visualization technologies were used to align annual BI scores with individual EHRs chronologically. Linear mixed-effects (LME) regression was conducted to model longitudinal BI scores based on socio-demographics, disease conditions, and features extracted from EHRs.

Results: The visualization presented a decline in BI scores changed by time and health history events. The LME model yielded a conditional R of 84%, a marginal R of 75%, and a Cohen's f of 0.68 in the design of random intercepts for individual heterogeneity. Changes in BI scores were significantly influenced by a set of socio-demographics (i.e., sex, education, living arrangement, and hobbies), disease conditions (i.e., dementia and diabetes mellitus), and EHRs features (i.e., event counts in allergies, diagnoses, accidents, wounds, hospital admissions, injections, etc.).

Conclusions: The proposed visualization approach and the LME model estimation can help to trace older adults' BI score changes and identify the influencing factors. The constructed long-term surveillance system provides reference data in clinical practice and help healthcare providers manage the time, cost, data and human resources in community-dwelling settings.
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http://dx.doi.org/10.1186/s12877-021-02422-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422750PMC
September 2021

Wedelolactone ameliorates Pseudomonas aeruginosa-induced inflammation and corneal injury by suppressing caspase-4/5/11/GSDMD-mediated non-canonical pyroptosis.

Exp Eye Res 2021 Sep 2;211:108750. Epub 2021 Sep 2.

Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Heilongjiang Province, PR China; Key Laboratory of Basic and Clinical Research of Heilongjiang Province, PR China. Electronic address:

Pseudomonas aeruginosa (P. aeruginosa) keratitis, a worldwide leading cause of corneal perforation and blindness, which is associated with contact lens usage. Increasing evidence has indicated that pyroptosis, a novel proinflammatory programmed cell death, is linked with ocular diseases, little is known about the role of noncanonical pyroptosis in microbial keratitis. Here, we first indicated the involvement of noncanonical pyroptosis in P. aeruginosa keratitis and investigated whether wedelolactone (WDL), a major active component of Eclipta prostrate known to target caspase-11, could alleviate P. aeruginosa keratitis development. We found the expression of caspase-4/5/11 and cleaved GSDMD in corneas of P. aeruginosa keratitis patients, animal models and lipopolysaccharide (LPS)-induced primary cultured human corneal keratocytes (piHCKs) were increased. Combining ciprofloxacin with WDL significantly ameliorated the severity of P. aeruginosa keratitis, as manifested by decreased inflammatory responses and reduced corneal epithelial defects. Consistent with these findings, WDL also dose-dependently alleviated LPS-induced noncanonical pyroptosis by reversing the increased expression of caspase-4/5 and GSDMD in piHCKs. In summary, our results demonstrated that by targeting the activation of caspase-4/5/11, wedelolactone inhibited the development of P. aeruginosa keratitis and suppressed the release of proinflammatory cytokines. Wedelolactone may be a promising anti-inflammatory candidate to combat P. aeruginosa keratitis.
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http://dx.doi.org/10.1016/j.exer.2021.108750DOI Listing
September 2021

Biomimetic mineralisation systems for in situ enamel restoration inspired by amelogenesis.

J Mater Sci Mater Med 2021 Aug 28;32(9):115. Epub 2021 Aug 28.

Department of Obsterics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin, China.

Caries and dental erosion are common oral diseases. Traditional treatments involve the mechanical removal of decay and filling but these methods are not suitable for cases involving large-scale enamel erosion, such as hypoplasia. To develop a noninvasive treatment, promoting remineralisation in the early stage of caries is of considerable clinical significance. Therefore, biomimetic mineralisation is an ideal approach for restoring enamel. Biomimetic mineralisation forms a new mineral layer that is tightly attached to the surface of the enamel. This review details the state-of-art achievements on the application of amelogenin and non-amelogenin, amorphous calcium phosphate, ions flow and other techniques in the biomimetic mineralisation of enamel. The ultimate goal of this review was to shed light on the requirements for enamel biomineralisation. Hence, herein, we summarise two strategies of biological minimisation systems for in situ enamel restoration inspired by amelogenesis that have been developed in recent years and compare their advantages and disadvantages.
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http://dx.doi.org/10.1007/s10856-021-06583-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403113PMC
August 2021

Age-related alteration in characteristics, function, and transcription features of ADSCs.

Stem Cell Res Ther 2021 Aug 23;12(1):473. Epub 2021 Aug 23.

Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, No.5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.

Background And Objectives: Adipose tissue-derived stem cells (ADSCs) autologous transplantation has been a promising strategy for aging-related disorders. However, the relationship between ADSCs senescence and organismal aging has not been clearly established. Therefore, we aimed at evaluating senescence properties of ADSCs from different age donors and to verify the influence of organismal aging on the proliferation and function of ADSCs in vitro, providing the theoretical basis for the clinical application of autologous ADSCs transplantation.

Methods And Results: The ADSCs were obtained from 1-month-old and 20-month-old mice. The cells characteristics, functions, gene expression levels, apoptosis proportion, cell cycle, SA-β-gal staining, and transcription features were evaluated. Compared to ADSCs from 1-month-old mice, ADSCs from 20-month-old mice exhibited some senescence-associated changes, including inhibited abilities to proliferate. Moreover, differentiation abilities, cell surface markers, and cytokines secreting differed between 1M and 20M ADSCs. SA-β-Gal staining did not reveal differences between the two donor groups, while cells exhibited more remarkable age-related changes through continuous passages. Based on transcriptome analysis and further detection, the CCL7-CCL2-CCR2 axis is the most probable mechanism for the differences.

Conclusions: ADSCs from old donors have some age-related alterations. The CCL7-CCL2-CCR2 axis is a potential target for gene therapy to reduce the harmful effects of ADSCs from old donors. To improve on autologous transplantation, we would recommend that ADSCs should be cryopreserved in youth with a minimum number of passages or block CCL7-CCL2-CCR2 to abolish the effects of age-related alterations in ADSCs through the Chemokine signaling pathway.
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http://dx.doi.org/10.1186/s13287-021-02509-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383427PMC
August 2021

Yiqi Huoxue Recipe Regulates Autophagy through Degradation of Advanced Glycation End Products via mTOR/S6K1/LC3 Pathway in Diabetic Nephropathy.

Evid Based Complement Alternat Med 2021 9;2021:9942678. Epub 2021 Aug 9.

Laboratory and Facilities Management Office, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.

Background: Yiqi Huoxue recipe can delay the progression of diabetic nephropathy, but its treatment mechanism is still unclear. We aimed to explore the effects of Yiqi Huoxue recipe on autophagy in diabetic nephropathy and its underlying mechanism.

Methods: All rats were randomly divided into seven groups. The body weight, kidney weight, blood glucose, glycated hemoglobin, urine protein, urine microprotein, creatinine, urea nitrogen, triglyceride, and lipoprotein were analyzed. HE, Masson, and periodic acid-Schiff staining were used to detect the severity of pathological changes in kidneys. The level of advanced glycation end products was assessed by the ELISA. Immunofluorescence staining was performed to check the expressions of podocin and nephrin. The expression levels of mTOR/S6K1/LC3 pathway-related proteins and mRNA were detected by qRT-PCR and western blotting.

Results: Yiqi Huoxue recipe significantly elevated body weight and significantly decreased kidney weight and kidney index. Yiqi Huoxue recipe significantly affected the levels of biochemical indicators in diabetic nephropathy and showed a regulatory effect on kidney damage and lipid metabolism disorders. ELISA showed that Yiqi Huoxue recipe significantly reduced the level of advanced glycation end products. The expressions of nephrin and podocin increased significantly, depending on the dosage of Yiqi Huoxue recipe. Additionally, Yiqi Huoxue recipe regulated the expression levels of p-AKT, mTOR, S6K1, and LC3.

Conclusion: Yiqi Huoxue recipe regulates podocyte autophagy to promote the degradation of advanced glycation end products through mTOR/S6K1/LC3 pathway. It has a certain guiding significance for the diagnosis and treatment of diabetic nephropathy.
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http://dx.doi.org/10.1155/2021/9942678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367537PMC
August 2021

Research Note: Development of rapid isothermal amplification assay for detection of duck circovirus.

Poult Sci 2021 Sep 24;100(9):101339. Epub 2021 Jun 24.

College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China. Electronic address:

Duck circovirus disease (DuCVD) caused by duck circovirus (DuCV) continues to spread in recent years, which brings serious harm to the poultry industry, so early diagnosis of DuCVD is of great significance for the prevention and control of this disease. Specific primers and probes for DuCV were designed in this study. Reverse primers and probes were modified at the 5' ends with biotin and fluorescein, respectively, and they were combined with dipsticks labeled with biotin antibodies and fluorescein antibodies to establish a recombinase-aided amplification-lateral flow dipstick (RAA-LFD) assay for detection of duck circovirus. By using this method, the reaction products reached detectable levels in about 20 min as a result of rapid amplification at a constant temperature of 37℃. The detection results could be observed by dripping the reaction products onto the dipstick within 2 to 3 min. The RAA-LFD method has good specificity and high sensitivity (10 copies/μL). Compared with conventional polymerase chain reaction (PCR), RAA-LFD has no power limit on the testing instrument, and is easy to use, saving more time and manpower, so it is more suitable for clinical detection.
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http://dx.doi.org/10.1016/j.psj.2021.101339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339307PMC
September 2021

Optical Coherence Tomography Angiography as a Noninvasive Assessment of Cerebral Microcirculatory Disorders Caused by Carotid Artery Stenosis.

Dis Markers 2021 5;2021:2662031. Epub 2021 Jul 5.

Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, China.

Purpose: Using retinal optical coherence tomography angiography (OCTA), we aimed to investigate the changes in important indicators of cerebral microcirculatory disorders, such as the properties of the radial peripapillary capillaries, vascular complexes, and the retinal nerve fiber layer, caused by carotid stenosis and postoperative reperfusion.

Methods: In this prospective longitudinal cohort study, we recruited 40 carotid stenosis patients and 89 healthy volunteers in the First Affiliated Hospital of Harbin Medical University (Harbin, China). Eyes with ipsilateral carotid stenosis constituted the experimental group, while the fellow eyes constituted the contralateral eye group. Digital subtraction angiography, CT perfusion imaging (CTP), and OCTA examinations were performed in all subjects. The vessel density of the radial peripapillary capillaries (RPC), superficial retinal vascular complexes (SVC), deep vascular complexes (DVC), choriocapillaris (CC), and the thickness of the retinal nerve fiber layer (RNFL) were assessed. Propensity-matched analysis was undertaken to adjust for covariate imbalances. Intergroup comparative analysis was conducted, and the paired sample -test was used to evaluate the preoperative and postoperative changes in OCTA variables.

Results: The ocular vessel density in the experimental group was significantly lower than that in the control group (RPC: 55.95 vs. 57.24, = 0.0161; SVC: 48.65 vs. 52.22, = 0.0006; DVC: 49.65 vs. 57.50, < 0.0001). Participants with severe carotid stenosis have reduced contralateral ocular vessel density (RPC 54.30; SVC 48.50; DVC 50.80). Unilateral stenosis removal resulted in an increase in vessel density on both sides, which was detected by OCTA on the 4 day (RPC, < 0.0001; SVC, = 0.0104; DVC, = 0.0104). Moreover, the ocular perfusion was consistent with that established by CTP.

Conclusion: OCTA can be used for sensitive detection and accurate evaluation of decreased ocular perfusion caused by carotid stenosis and may thus have the potential for application in noninvasive detection of cerebral microcirculation disorders. This trial is registered with NCT04326842.
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http://dx.doi.org/10.1155/2021/2662031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277520PMC
July 2021

Analysing wastewater to estimate fentanyl and tramadol use in major Chinese cities.

Sci Total Environ 2021 Nov 4;795:148838. Epub 2021 Jul 4.

Queensland Alliance for Environmental Health Sciences (QAEHS), The University of Queensland, Brisbane, Queensland 4102, Australia.

The misuse of fentanyl and more recently tramadol in the population has caused an opioid crisis in several countries and drawn much public attention worldwide. However, there is a gap of information on the potential misuse of fentanyl and tramadol in China. This study aims to fill this gap by analysing fentanyl and tramadol in wastewater of major cities across China to estimate their use. Wastewater samples were collected from 30 cities located across all seven geographic regions of China, from 2016 to 2019. Fentanyl was detected in only a few samples, suggesting a low prevalence of this potent opioid drug in China. Meanwhile, tramadol was found in most samples with concentrations ranging up to 186 ng/L. The per capita daily consumption of tramadol estimated from wastewater across China ranged from 6 mg/d/1000 in. to 213 mg/d/1000 inh. The consumption of tramadol seems to be similar among all the days of the week. Tramadol use is overall higher in Northeast China than in other regions, which is different from heroin, another popular opioid in China. Temporally, there is a significant decrease in tramadol use in major cities of China from 2016 to 2019. The results of our study suggested that tramadol use in China was predominantly from pharmaceutical prescription and not as prevalent as in other countries.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148838DOI Listing
November 2021

First report of leaf blight on Magnolia coco caused by Nothophoma quercina in China.

Plant Dis 2021 Jul 2. Epub 2021 Jul 2.

chengdu, China;

Magnolia coco (Lour.) DC. is an ornamental shrub and widely cultivated in southern China (Nana et al. 2017). In April 2020, leaf blight symptoms were observed on the leaves of M. coco in the Chengdu campus of Sichuan Agricultural University (30°42'19.92″N, 103°51'30.61″E, 493 m) where didn't have great protection, with roughly 70% leaves per plant were diseased. The initial symptoms presented on the leaf apex, which was manifested as dark brown spots surrounded with obvious yellowish halo (Fig. 1). As the disease progressed, spots gradually enlarged and coalesced covering the leaf, and severe infection finally caused leaf necrosis and plant decline. Four specimens from different diseased plants were used for pathogen isolation and morphological observation. Four fungal isolates were obtained from four specimens, following Chomnunti et al. (2014). Colonies on potato dextrose agar (PDA) medium were initially white and then light brown to dark brown. Pycnidia measured 284-427 × 326-554 μm (x=372.8 μm × 476.1 μm, n=20), and were brownish-black to black, broadly globose to irregular. The pycnidial wall measured 16-27 μm wide (n=20) and was composed of hyaline to brown cells of textura angularis. Conidiophores were absent, and the conidiogenous cells are pear-shaped, colorless, and smooth. Conidia measured 5-8 × 4-6 μm (x=6.5 μm × 4.6 μm, n=50), and were elliptical or subglobose, thick-walled, aseptate, hyaline, smooth, brown. These asexual structures were similar to Nothophoma quercina (Syd. & P. Syd.) Qian Chen & L. Cai described by Chen et al. (2017). The genomic DNA of representative isolate SICAUCC 21-0011 was extracted, and the internal transcribed spacers (ITS), 28S large subunit rDNA (LSU), RNA polymerase II large subunit 2 (RPB2), and beta-tubulin (TUB2) regions were amplified using the primer pairs ITS5/ITS4, LR0R/LR5, FRPB2-5F/FRPB2-7cR, and T1/BT4R, respectively. The accession numbers deposited in GenBank were MW541930 (ITS), MW541934 (LSU), MW883395 (RPB2), and MW883394 (TUB2). Nucleotide BLAST showed high homology with the sequences of N. quercina, viz. GU237900 (ITS, 485/486, 99.79%), EU754127 (LSU, 862/862, 100%), KT389657 (RPB2, 593/596, 99.49%), and GU237609 (TUB2, 333/335, 99.40%). Phylogenetic analyses based on a combined dataset showed 100% bootstrap support values in a clade with N. quercina complexes (Fig. 2). Four healthy potted plants (2-years-old) with 15 to 20 leaves per plant were sprayed with conidial suspension (105 conidia/mL) prepared from 4-week-old cultures of SICAUCC 21-0011, which incubated on PDA at 25℃, onto the wounded sites via pin-prick inoculation described by Desai et al. (2019). Another four plants were sprayed with sterilely distilled water as controls. Inoculated plants were cultured in a growth chamber (25℃, 95% relative humidity, and 12-h photoperiod). About 30 days later, brown spots were found on the inoculated leaves, which were similar to those observed in the field. There were no symptoms on the control plants, and the pathogen was re-isolated from the diseased leaves and characterized morphologically. N. quercina has been reported on Photinia × fraseri Dress, Aucuba japonica, Malus micromalus, and Chaenomeles sinensis (Mohamed et al. 2019, Lv et al. 2020, Zou et al. 2021). To our knowledge, this is the first report of leaf blight on M. coco caused by N. quercina. M. coco is one of the important ornaments in the courtyard, street, and park in China, and the risk of this pathogen needs further exploration and effective control measures should be made. Qian Zeng, Yicong Lv, and Xinyue Li contributed equally to this work.
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http://dx.doi.org/10.1094/PDIS-05-21-1061-PDNDOI Listing
July 2021

First report of brown leaf spot on caused by in Sichuan, China.

Plant Dis 2021 Jun 25. Epub 2021 Jun 25.

Sichuan Agricultural University, 12529, Chengdu, Sichuan, China;

Dode (Iron walnut) is mostly distributed in southwestern China, and valued for wood and nuts (Feng et al. 2018). In April 2020, we surveyed a walnut garden located in Baisha Town, Wanyuan City, (Sichuan, China), where brown spot symptoms were observed on leaves of ten trees among of 100 plants, and this disease can result in a reduced growth potential when trees are severely infected. Necrotic and subcircular lesions with conidiamata were observed on diseased leaves. Symptomatic leaves were collected and taken back to the laboratory forfurther analysis. Using the single spore isolation technique developed by Chomnunti et al. (2014), five isolates were grown from the infected leaves on Potato Dextrose Agar medium (PDA). The five isolates had similar colony morphology, which was initially white, suborbicular, gradually turning yellowish with black spots, developing fluffy aerial mycelium. Morphological characteristics were examined using light microscopy on the PDA. Conidiogenous cells were subcylindrical to cylindrical, or ampulliform, hyaline, rarely branched. Macroconidia were lunate, reniform, hyaline, 1-3-septate, mostly 1-septate, distinctly constricted at the septum, the basal cell was bluntly rounded, the apical cell had an acute end, and the basal cell was equal to or larger than the apical cell, measuring 22 to 40.5 × 2.5 to 8.3 μm (mean = 32 × 6.2 μm, n = 50). Microconidia were botuliform, or subfusiform, hyaline, both ends were rounded, straight or curved, aseptate, and measured 10 to 28.5 × 1.9 to 3.7 μm (mean= 17.2 × 2.7 μm, n = 20). A multilocus approach was conducted for precise identification of a representative isolate SICAUCC 20-0012. The internal transcribed spacer regions (ITS), guanine nucleotide-binding protein subunit beta gene (MS204), and translation elongation factor 1-alpha (tef1-α) of isolate SICAUCC 20-0012 were amplified and sequenced as described by Sogonov et al. (2008) and Walker et al. (2012a). GenBank Accession Nos. for ITS, MS204, and tef1-α are MW250303, MW246773, and MW246775, respectively. Phylogenetic analyses showed 100% support with (Fr.) Sogonov, and the morphology was consistent with the asexual stage of documented by Walker et al. (2012b). To test Koch's postulates, five healthy plants of (2- to 3-year-old) with 5-8 leaves per plant were inoculated with conidial suspensions (10 conidia/mL) after wounded with a small pin as described by Desai et al. (2019), and the same number of healthy plants were wounded and sprayed with sterile distilled water as controls. Plants were sprayed regularly with distilled water every day and placed in a growth chamber at 25℃ with a 12-h fluorescent light/dark regimen. After 15 days, typical brown spot symptoms developed on inoculated leaves, but not on the controls. The fungus was reisolated from the lesion as described above but not from non-inoculated leaves. has been reported on some walnut trees; for example: , , , , , , , and (Farr & Rossman 2020). However, to our knowledge, this is the first report of causing brown leaf spot on . is an economically important tree in southwest China, and fungicide treatments should be considered to prevent the spread of this fungus before it becomes more widespread. Chunlin Yang, Yu Deng, and Feihu Wang contributed equally to this work. This research was supported by the Key Research and Development Project of Sichuan Province (2021YFYZ0032).
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http://dx.doi.org/10.1094/PDIS-02-21-0344-PDNDOI Listing
June 2021

Characterization of an Acidic Polysaccharides from Carrot and Its Hepatoprotective Effect on Alcoholic Liver Injury in Mice.

Chem Biodivers 2021 Aug 13;18(8):e2100359. Epub 2021 Jul 13.

Bioscience and Biotechnology College, Shenyang Agriculture University, 120 Dongling Road, Shenyang, 110866, P. R. China.

The characteristics of acidic polysaccharides extracted from Daucus carota L. var. sativa Hoffm were investigated and its hepatoprotective effects on alcoholic liver injury were determined in the mice model. A carrot polysaccharide (CPS-I: Carrot polysaccharide-I) with the molecular weight of 3.40×10  kDa was isolated from Daucus carota L. and purified by diethylaminoethyl-52 and Sephadex G-150 column chromatography. The components were analyzed by HPLC, which revealed that CPS-I consisted of galacturonic acid, rhamnose, xylose, arabinose, fructose, and galactose at a relative ratio of 1 : 3.16 : 1.13 : 5.53 : 3.45 : 7.76. Structural characterization analysis suggested that CPS-I was mainly composed of →6)-β-D-Galp-(1→ and →5)-α-L-Araf-(1→. The hepatoprotective effect of CPS-I was evaluated by alcoholic liver injury mice model. The results showed that the administration of CPS-I (300 mg/kg/day) alleviated the alcoholic liver injury in mice by increasing the levels of ADH and ALDH and reducing oxidative stress. CPS-I ameliorated the pathological changes of liver characterized by lipid accumulation, and reduced the number of lipid droplets.
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http://dx.doi.org/10.1002/cbdv.202100359DOI Listing
August 2021

Estimation of komatsuna freshness using visible and near-infrared spectroscopy based on the interpretation of NMR metabolomics analysis.

Food Chem 2021 Dec 16;364:130381. Epub 2021 Jun 16.

Food Research Institute, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8642, Japan. Electronic address:

The objective of this study was to explore the potentiality and mechanism of visible and near-infrared (Vis-NIR) spectroscopy in estimating the freshness of komatsuna. We monitored the cumulative CO production of komatsuna stored under different conditions as a freshness indicator and measured the Vis-NIR spectra of komatsuna as the predictor. Using the informative wavelengths (IW) selected using the stepwise selectivity ratio method, we constructed an accurate freshness prediction model through PLSR analysis. The IW in the visible region were attributed to pigments such as chlorophyll. In the NIR region, ten amino acids were identified as directly or indirectly contributing to the IW and were highly related to freshness. They were confirmed on the basis of the strong correlations between the informative NIR signals and NMR signals, which were determined using statistical heterospectroscopy. The results demonstrate the feasibility of Vis-NIR spectroscopy in estimating the freshness of komatsuna using the IW.
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http://dx.doi.org/10.1016/j.foodchem.2021.130381DOI Listing
December 2021

Red-light-responsive coordination polymers nanorods: New strategy for ultrasensitive photothermal detection of targeted cancer cells.

Biosens Bioelectron 2021 Oct 8;190:113417. Epub 2021 Jun 8.

State Key Laboratory of Applied Organic Chemistry and Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Gansu Lanzhou, 730000, PR China. Electronic address:

The development of highly sensitive and simple detection methods for cancer cells is an important challenge to achieve early cancer diagnosis and effective treatment. In this paper, folic acid (FA)-conjugated platinum (IV) methylene blue (MB) coordination polymers nanorods (denoted as FA-PtCPs NRs) were developed by the photochemical method. The structure of the PtCPs NRs was investigated using the meta-dynamics and genetic algorithms (MTD-GC) method, and it was found that the coordination bond was formed between platinum (IV) and N atoms of MB. The field emission scanning electron microscope (FE-SEM) and transmission electron microscope (TEM) indicated that the morphology of PtCPs NRs was rod-like. The resulting FA-PtCPs NRs was used for the specific and ultra-sensitive temperature detection of cancer cells based on PtCPs NRs as a signal trigger unit and FA as a target recognition tool. After three-step reaction, oxidized 3,3',5,5'-tetramethylbenzidine (ox-TMB) with photothermal effect was obtained. Under 660 nm laser irradiation, such detection platform can convert the molecular recognition signal between FA and folate receptor (FR) of cancer cells into readable temperature value, which can be directly read by an ordinary thermometer, with a detection limit as low as 2 cells/mL. In addition, FA-PtCPs NRs could be used as fluorescent probes for in-situ bioimaging. Therefore, this photothermal sensing platform has a broad prospect in the field of point-of-care detection of cancer cells.
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http://dx.doi.org/10.1016/j.bios.2021.113417DOI Listing
October 2021

Quantitative analysis of retinal vessel density and thickness changes in diabetes mellitus evaluated using optical coherence tomography angiography: a cross-sectional study.

BMC Ophthalmol 2021 Jun 15;21(1):259. Epub 2021 Jun 15.

Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Background: Diabetic retinopathy is the most common microvascular complication of diabetes; however, early changes in retinal microvessels are difficult to detect clinically, and a patient's vision may have begun to deteriorate by the time a problem is identified. Optical coherence tomography angiography (OCTA) is an innovative tool for observing capillaries in vivo. The aim of this study was to analyze retinal vessel density and thickness changes in patients with diabetes.

Methods: This was a retrospective, observational cross-sectional study. Between August 2018 and February 2019, we collected OCTA data from healthy participants and diabetics from the First Affiliated Hospital of Harbin Medical University. Analyzed their retinal vessel density and thickness changes.

Results: A total of 97 diabetic patients with diabetes at different severity stages of diabetic retinopathy and 85 controls were involved in the experiment. Diabetic patients exhibited significantly lower retinal VD (particularly in the deep vascular complexes), thickening of the neurosensory retina, and thinning of the retinal pigment epithelium compared with controls. In the control group, nondiabetic retinopathy group and mild diabetic retinopathy group, superficial VD was significantly correlated with retinal thickness (r = 0.3886, P < 0.0001; r = 0.3276, P = 0.0019; r = 0.4614, P = 0.0024, respectively).

Conclusions: Patients with diabetes exhibit ischemia of the retinal capillaries and morphologic changes in vivo prior to vision loss. Therefore, OCTA may be useful as a quantitative method for the early detection of diabetic retinopathy.
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http://dx.doi.org/10.1186/s12886-021-01988-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207746PMC
June 2021

First Report of Brown Leaf Spot of Caused by in China.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

chengdu, China;

"Chuanzao 2" is a walnut variety derived from the hybridization of L. and Dode distributed in southwest China, where it is an economically important tree species in rural regions (Xiao et al. 2012). In April 2020, the variety in a walnut garden showed symptoms of brown leaf spot in Beishan Town (107°21'43.93″E, 31°28'12.34″N), Dazhou City in Sichuan, China, with 5% to 10% of leaves per plant affected (5 plants). Symptomatic leaves showed brown to dark brown spots (2 to 5 mm) with a dark brown to black halo and grayish-tan center. The spots were subcircular to irregular in shape, and gradually expanded and formed necrotic spots. A single conidium isolation was performed (Senanayake et al. 2020) and transferred to Potato Dextrose Agar (PDA). Five isolates were obtained from five different infected leaves. Colonies of five isolates were subcircular, erose or dentate, flat or effuse, white initially, gradually becoming yellowish with white margins, developed and fluffy aerial mycelia, and conidiogenensis was produced underneath mycelia after 25-days-incubation. Conidiogenous cells were subcylindrical to cylindrical, or irregular in shape, and hyaline. Macroconidia were lunate, reniform, hyaline, basal cell bluntly rounded, apical cell with acute end, 1-septate, rarely aseptate, sometimes slightly constricted at septum, basal cell equal or larger than apical cell, and measured 16.5 to 30.5 × 5 to 8.5 μm (mean = 23.2 × 6.3 μm, n = 50). Microconidia were not observed. These morphological characteristics resembled those of (Fr.) Sogonov (Walker et al. 2012a). For molecular identification, genomic DNA (isolates SICAUCC 21-0008 and SICAUCC 21-0010) was extracted, and the internal transcribed spacers (ITS) region, guanine nucleotide-binding protein subunit beta (MS204) gene, and translation elongation factor 1-alpha (tef1-α) were amplified and sequenced by using the primers ITS5/ITS4 (White et al. 1990), E1F1/E5R1a (Walker et al. 2012a), and EF1-728F/EF1-1567R (Walker et al. 2012b), respectively. Phylogenetic analyses (maximum likelihood) based on a combined dataset showed 100% bootstrap support values in a clade with . The sequences of ITS, MS204, and tef1-α genes were deposited in GenBank with accession numbers MW493111/MZ026300, MW495270/MZ031975, and MW495271/MZ031974, respectively. To fulfill Koch's postulates, five healthy hybrid plants (2 to 3 years old) with 5 to 8 leaves per plant were spray inoculated with conidium suspensions (10 conidia/mL; isolate SICAUCC 21-0008) prepared from 40-days-old cultures onto the wounded sites via pin-prick inoculation. Similarly, five noninoculated plants sprayed with sterile water served as controls. Plants were placed in a growth chamber at 25℃ on a 12-h fluorescent light/dark regime and daily sprayed with sterile distilled water. After two weeks, observed symptoms were similar to those from natural infections. No disease symptoms were found on control plants. The fungus was reisolated from the diseased leaves and characterized morphologically. is a global pathogen and has been reported to cause the leaf spot in many walnut trees, viz. , , , and , etc. To our knowledge, this is the first report of causing brown leaf spot on ( × ) in China. The increasing risk of this pathogen in the walnut-growing areas of Sichuan Province of China needs a further exploration and outreach effort to develop effective control measures. Chunlin Yang, Feng Liu, and Qian Zeng contributed equally to this paper.
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http://dx.doi.org/10.1094/PDIS-05-21-0981-PDNDOI Listing
June 2021

The Amygdala Responds Rapidly to Flashes Linked to Direct Retinal Innervation: A Flash-evoked Potential Study Across Cortical and Subcortical Visual Pathways.

Neurosci Bull 2021 Aug 4;37(8):1107-1118. Epub 2021 Jun 4.

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500, China.

Rapid detection and response to visual threats are critical for survival in animals. The amygdala (AMY) is hypothesized to be involved in this process, but how it interacts with the visual system to do this remains unclear. By recording flash-evoked potentials simultaneously from the superior colliculus (SC), lateral posterior nucleus of the thalamus, AMY, lateral geniculate nucleus (LGN) and visual cortex, which belong to the cortical and subcortical pathways for visual fear processing, we investigated the temporal relationship between these regions in visual processing in rats. A quick flash-evoked potential (FEP) component was identified in the AMY. This emerged as early as in the LGN and was approximately 25 ms prior to the earliest component recorded in the SC, which was assumed to be an important area in visual fear. This quick P1 component in the AMY was not affected by restraint stress or corticosterone injection, but was diminished by RU38486, a glucocorticoid receptor blocker. By injecting a monosynaptic retrograde AAV tracer into the AMY, we found that it received a direct projection from the retina. These results confirm the existence of a direct connection from the retina to the AMY, that the latency in the AMY to flashes is equivalent to that in the sensory thalamus, and that the response is modulated by glucocorticoids.
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http://dx.doi.org/10.1007/s12264-021-00699-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353027PMC
August 2021

One-Dimensional Frenkel Chain Defects in CsBiTe.

J Phys Chem Lett 2021 Jun 1;12(22):5319-5323. Epub 2021 Jun 1.

International Center for Quantum Design of Functional Materials (ICQD), Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic China.

Understanding the detailed process of spontaneous formation of intrinsic defects and their ability to tune the electronic structures in functional materials has become a key prerequisite for their technological applications. Here, by using scanning tunneling microscopy, we report the observation of one-dimensional Frenkel chain defects on the cleaved CsBiTe surface due to the migration of Te atoms for the first time. Further scanning tunneling spectroscopy measurements clearly revealed a self-electron doping effect of the Frenkel chain defects, which could directly affect their thermoelectric and superconducting properties. The unique one-dimensional Frenkel tellurium atomic chain defect and its doping effect on the electronic structure observed here not only shed light on tuning the electric properties of a series of tellurides but also possess profound implications for enriching the microscopic details of defect chemistry and materials science.
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http://dx.doi.org/10.1021/acs.jpclett.1c01239DOI Listing
June 2021

Genome-wide analysis of ARF transcription factors reveals HcARF5 expression profile associated with the biosynthesis of β-ocimene synthase in Hedychium coronarium.

Plant Cell Rep 2021 Jul 29;40(7):1269-1284. Epub 2021 May 29.

The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.

Key Message: Herein, 37 ARF genes were identified and analyzed in Hedychium coronarium and HcARF5 showed a potential role in the regulation of HcTPS3. Auxin is an important plant hormone, implicated in various aspects of plant growth and development processes especially in the biosynthesis of various secondary metabolites. Auxin response factors (ARF) belong to the transcription factors (TFs) gene family and play a crucial role in transcriptional activation/repression of auxin-responsive genes by directly binding to their promoter region. Nevertheless, whether ARF genes are involved in the regulatory mechanism of volatile compounds in flowering plants is largely unknown. β-ocimene is a key floral volatile compound synthesized by terpene synthase 3 (HcTPS3) in Hedychium coronarium. A comprehensive analysis of H. coronarium genome reveals 37 candidate ARF genes in the whole genome. Tissue-specific expression patterns of HcARFs family members were assessed using available transcriptome data. Among them, HcARF5 showed a higher expression level in flowers, and significantly correlated with the key structural β-ocimene synthesis gene (HcTPS3). Furthermore, transcript levels of both genes were associated with the flower development. Under hormone treatments, the response of HcARF5 and HcTPS3, and the emission level of β-ocimene contents were evaluated. Subcellular and transcriptional activity assay showed that HcARF5 localizes to the nucleus and possesses transcriptional activity. Yeast one-hybrid (Y1H) and dual-luciferase assays revealed that HcARF5 directly regulates the transcriptional activity of HcTPS3. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that HcARF5 interacts with scent-related HcIAA4, HcIAA6, and HcMYB1 in vivo. Overall, these results indicate that HcARF5 is potentially involved in the regulation of β-ocimene synthesis in H. coronarium.
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http://dx.doi.org/10.1007/s00299-021-02709-1DOI Listing
July 2021

Management of high-grade squamous intraepithelial lesion patients with positive margin after LEEP conization: A retrospective study.

Medicine (Baltimore) 2021 May;100(20):e26030

Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong, PR China.

Abstract: To explore the optimal way to manage patients with high-grade squamous intraepithelial lesion (HSIL) and positive margin by identifying the risk factors for its recurrence and residue.A retrospective study was conducted on 267 cases of a pathologically confirmed HSIL with positive margin following conization by loop electrosurgical excisional procedure (LEEP) between January 2010 and December 2015. One hundred two cases were selected for regular follow-up every 6 months, and 165 cases were selected for a second surgery (repeat cervical conization or hysterectomy) within 3 months of initial LEEP. We analyzed the association between recurrent or residual diseases and these factors: age, menopausal status, ThinPrep cytologic test (TCT) results, high-risk human papillomavirus (HR-HPV) infection, pathological grades of the margin, number of involved margins, and glandular involvement.The recurrence rate among 102 cases who underwent follow-up was 17.6% (18/102). The factors: atypical squamous cells of undetermined significance cannot exclude HSIL (ASC-H) or higher lesions in the pre-LEEP TCT (P = .038), persistent HR-HPV infection at the 6th month post-LEEP (P = .03), HSIL-positive margin (P = .003), and multifocal-involved margin (P = .002) were significantly associated with recurrent disease, while age, menopause, and pre-LEEP HR-HPV infection were not associated with recurrent disease (P > .05). The residual rate among 165 patients who underwent a second surgery was 45.5% (75/165), of which 15 cases were residual cervical cancer. The factors: menopause (P = .02), ≥ASC-H in pre-LEEP TCT (P = .04), pre-LEEP HR-HPV infection (P = .04), ≥HSIL-positive margin (P < .001), and multifocal-involved margin (P < .001) significantly increased the risk of residual disease. No correlation existed between residual disease and age or glandular involvement (P > .05).For patients with a positive margin after LEEP, regular follow-up or second surgery should be selected according to fertility requirement and pathological characteristics of the positive margin, as well as TCT and HR-HPV infection condition.
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http://dx.doi.org/10.1097/MD.0000000000026030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137043PMC
May 2021

Early risk factors for extrapulmonary organ injury in adult COVID-19 patients.

Ann Transl Med 2021 Apr;9(8):701

Department of Intensive Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: The novel 2019 coronavirus (COVID-19) has caused a global pandemic, and often leads to extrapulmonary organ injury. However, the risk factors for extrapulmonary organ injury are still unclear. We aim to explore the risk factors for extrapulmonary organ injury and the association between extrapulmonary organ injury and the prognosis in COVID-19 patients.

Methods: We implemented a single-center, retrospective, observational study, in which a total of 349 confirmed COVID-19 patients admitted to Tongji Hospital from January 25, 2020, to February 25, 2020, were enrolled. We collected demographic, clinical, laboratory, and treatment data from electronic medical records. Potential risk factors for extrapulmonary organ injury of COVID-19 patients were analyzed by a multivariable binary logistic model, and multivariable Cox proportional hazards regression model was used for survival analysis in the patients with extrapulmonary organ injury.

Results: The average age of the included patients was 61.73±14.64 years. In the final logistic model, variables including aged 60 or older [odds ratio (OR) 1.826, 95% confidence interval (CI): 1.060-3.142], acute respiratory distress syndrome (ARDS) (OR 2.748, 95% CI: 1.051-7.185), lymphocytes count lower than 1.1×10/L (OR 0.478, 95% CI: 0.240-0.949), level of interleukin-6 (IL-6) greater than 7 pg/mL (OR 1.664, 95% CI: 1.005-2.751) and D-Dimer greater than 0.5 μg/mL (OR 2.190, 95% CI: 1.176-4.084) were significantly associated with the extrapulmonary organ injury. Kaplan-Meier curve and log-rank test showed that the probabilities of survival for patients with extrapulmonary organ injury were significantly lower than those without extrapulmonary organ injury. Multivariate Cox proportional hazards model showed that only myocardial injury (P=0.000, HR: 5.068, 95% CI: 2.728-9.417) and circulatory system injury (P=0.000, HR: 4.076, 95% CI: 2.216-7.498) were the independent factors associated with COVID-19 patients' poor prognosis.

Conclusions: Older age, lymphocytopenia, high level of D-Dimer and IL-6, and the severity of lung injury were the high-risk factors of extrapulmonary organ injury in COVID-19 patients. Myocardial and circulatory system injury were the most important risk factors related to poor outcomes of COVID-19 patients. It may help clinicians to identify extrapulmonary organ injury early and initiate appropriate treatment.
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http://dx.doi.org/10.21037/atm-21-1561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106092PMC
April 2021

Effectiveness and mechanisms of adipose-derived stem cell therapy in animal models of Parkinson's disease: a systematic review and meta-analysis.

Transl Neurodegener 2021 04 29;10(1):14. Epub 2021 Apr 29.

NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, 100021, China.

Animal models provide an opportunity to assess the optimal treatment way and the underlying mechanisms of direct clinical application of adipose-derived stem cells (ADSCs). Previous studies have evaluated the effects of primitive and induced ADSCs in animal models of Parkinson's disease (PD). Here, eight databases were systematically searched for studies on the effects and in vivo changes caused by ADSC intervention. Quality assessment was conducted using a 10-item risk of bias tool. For the subsequent meta-analysis, study characteristics were extracted and effect sizes were computed. Ten out of 2324 published articles (n = 169 animals) were selected for further meta-analysis. After ADSC therapy, the rotation behavior (10 experiments, n = 156 animals) and rotarod performance (3 experiments, n = 54 animals) were improved (P < 0.000 01 and P = 0.000 3, respectively). The rotation behavior test reflected functional recovery, which may be due to the neurogenesis from neuronally differentiated ADSCs, resulting in a higher pooled effect size of standard mean difference (SMD) (- 2.59; 95% CI, - 3.57 to - 1.61) when compared to that of primitive cells (- 2.18; 95% CI, - 3.29 to - 1.07). Stratified analyses by different time intervals indicated that ADSC intervention exhibited a long-term effect. Following the transplantation of ADSCs, tyrosine hydroxylase-positive neurons recovered in the lesion area with pooled SMD of 13.36 [6.85, 19.86]. Transplantation of ADSCs is a therapeutic option that shows long-lasting effects in animal models of PD. The potential mechanisms of ADSCs involve neurogenesis and neuroprotective effects. The standardized induction of neural form of transplanted ADSCs can lead to a future application in clinical practice.
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http://dx.doi.org/10.1186/s40035-021-00238-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081767PMC
April 2021

Aggregation Induced Emission and Nonlinear Optical Properties of an Intramolecular Charge-Transfer Compound.

Materials (Basel) 2021 Apr 11;14(8). Epub 2021 Apr 11.

School of Materials Science and Engineering, National Institute for Advanced Materials, Nankai University, Tongyan Road 38, Tianjin 300350, China.

Intramolecular charge transfer (ICT) compounds have attracted wide attention for their potential applications in optoelectronic materials and devices such as fluorescent sensors, dye-sensitized solar cells, organic light emitting diodes and nonlinear optics. In this work, we have synthesized a new ICT compound, dimethyl-[4-(7-nitro-benzo[1,2,5]thiadiazol-4-yl)-phenyl]-amine (BTN), and have fabricated it into low dimensional micro/nano structures with well-defined morphologies. These self-assembled nanostructures exhibit high efficiency solid state fluorescence via an aggregation induced emission mechanism, which overcomes the defect of fluorescence quenching caused by aggregation in the solid state of traditional luminescent materials. We also explored and studied the nonlinear optical properties of this material through the Z-scan method, and found that this material exhibits large third-order nonlinear absorption and refraction coefficients, which promises applications of the materials in the fields of nonlinear optics and optoelectronics.
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http://dx.doi.org/10.3390/ma14081909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069476PMC
April 2021

Coordinated and High-Level Expression of Biosynthetic Pathway Genes Is Responsible for the Production of a Major Floral Scent Compound Methyl Benzoate in .

Front Plant Sci 2021 7;12:650582. Epub 2021 Apr 7.

The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China.

Methyl benzoate is a constituent of floral scent profile of many flowering plants. However, its biosynthesis, particularly in monocots, is scarcely reported. The monocot is a popular ornamental plant in tropical and subtropical regions partly for its intense and inviting fragrance, which is mainly determined by methyl benzoate and monoterpenes. Interestingly, several related species lack floral scent. Here, we studied the molecular mechanism of methyl benzoate biosynthesis in . The emission of methyl benzoate in was found to be flower-specific and developmentally regulated. As such, seven candidate genes associated with methyl benzoate biosynthesis were identified from flower transcriptome of and isolated. Among them, HcBSMT1 and HcBSMT2 were demonstrated to catalyze the methylation of benzoic acid and salicylic acid to form methyl benzoate and methyl salicylate, respectively. Methyl salicylate is a minor constituent of floral scent. Kinetic analysis revealed that HcBSMT2 exhibits a 16.6-fold lower m value for benzoic acid than HcBSMT1, indicating its dominant role for floral methyl benzoate formation. The seven genes associated with methyl benzoate biosynthesis exhibited flower-specific or flower-preferential expression that was developmentally regulated. The gene expression and correlation analysis suggests that and play critical roles in the regulation of methyl benzoate biosynthesis. Comparison of emission and gene expression among four species suggested that coordinated and high-level expression of biosynthetic pathway genes is responsible for the massive emission of floral methyl benzoate in . Our results provide new insights into the molecular mechanism for methyl benzoate biosynthesis in monocots and identify useful molecular targets for genetic modification of scent-related traits in .
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http://dx.doi.org/10.3389/fpls.2021.650582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058416PMC
April 2021

Genome-wide identification and expression pattern of SnRK gene family under several hormone treatments and its role in floral scent emission in .

PeerJ 2021 10;9:e10883. Epub 2021 Mar 10.

The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangdong, China.

The (Snf1-Related protein Kinase) gene family plays crucial roles in various plant signaling pathways and stress-adaptive responses including biotic and abiotic stresses via activating protein phosphorylation pathways. However, there is no information available on the role of the gene family in . is an important crop widely cultivated as an ornamental plant, herb, spice, or condiment. In this study, 60 genes were identified from the genomic and transcriptome data. Phylogenetic and gene structure analysis showed that the genes were divided into three groups (, and ) and among them subfamily was further subdivided into two clades according to the number of introns. Chromosome localization analysis showed that genes were unevenly mapped onto all chromosomes, and the ratio of 24 paralogues includes four tandems and 20 segmental duplications indicated that the gene family underwent a purifying selection. -regulatory elements analysis suggested that the genes respond to multiple hormones and other stresses. The responsiveness of genes to several hormones was analyzed by quantitative real-time PCR. Based on the different transcriptome data, two candidates genes ( and ) were screened out for further characterization . The subcellular localization experiment revealed that both genes were located in the nucleus and cytoplasm. Moreover, virus-induced gene silencing (VIGS) of and significantly reduced the floral volatile contents by suppressing the expression of terpene synthase genes ( and ), indicating that and genes play an important role in the regulatory mechanism of floral aroma. These results will provide novel insights into the functional dissection of gene family.
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http://dx.doi.org/10.7717/peerj.10883DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955670PMC
March 2021

The Transmembrane Mucin MUC1 Facilitates β1-Integrin-Mediated Bacterial Invasion.

mBio 2021 04 6;12(2). Epub 2021 Apr 6.

Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands

At the intestinal host-microbe interface, the transmembrane mucin MUC1 can function as a physical barrier as well as a receptor for bacteria. MUC1 also influences epithelial cell morphology and receptor function. Various bacterial pathogens can exploit integrins to infect eukaryotic cells. It is yet unclear whether MUC1 influences the interaction of bacteria with integrins. We used expressing the invasin (inv) protein of ( inv) to assess the effects of MUC1 on β1 integrin (ITGB1)-mediated bacterial invasion. Our results show that expression of full-length MUC1 does not yield a physical barrier but slightly enhances inv uptake. Enzymatic removal of the MUC1 extracellular domain (ED) using a secreted protease of C1 esterase inhibitor (StcE) of pathogenic had no additional effect on inv invasion. In contrast, expression of a truncated MUC1 that lacks the cytoplasmic tail (CT) reduced bacterial entry substantially. Substitution of tyrosine residues in the MUC1 CT also reduced bacterial uptake, while deletion of the C-terminal half of the cytoplasmic tail only had a minor effect, pointing to a regulatory role of tyrosine phosphorylation and the N-terminal region of the MUC1 CT in integrin-mediated uptake process. Unexpectedly, StcE removal of the ED in MUC1-ΔCT cells reversed the block in bacterial invasion. Together, these findings indicate that MUC1 can facilitate β1-integrin-mediated bacterial invasion by a concerted action of the large glycosylated extracellular domain and the membrane-juxtaposed cytoplasmic tail region. Bacteria can exploit membrane receptor integrins for cellular invasion, either by direct binding of bacterial adhesins or utilizing extracellular matrix components. MUC1 is a large transmembrane glycoprotein expressed by most epithelial cells that can have direct defensive or receptor functions at the host-microbe interface and is involved in facilitating integrin clustering. We investigated the role of epithelial MUC1 on β1 integrin-mediated bacterial invasion. We discovered that MUC1 does not act as a barrier but facilitates bacterial entry through β1 integrins. This process involves a concerted action of the MUC1 -glycosylated extracellular domain and cytoplasmic tail. Our findings add a new dimension to the complexity of bacterial invasion mechanisms and provide novel insights into the distinct functions of MUC1 domains at the host-microbe interface.
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http://dx.doi.org/10.1128/mBio.03491-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092303PMC
April 2021

Gut Microbiota-Derived Trimethylamine N-Oxide and Kidney Function: A Systematic Review and Meta-Analysis.

Adv Nutr 2021 07;12(4):1286-1304

Department of Endocrinology and Metabolism, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.

Elevated circulating trimethylamine N-oxide (TMAO) concentrations have been observed in patients with chronic kidney disease (CKD). We aimed to systematically estimate and quantify the association between TMAO concentrations and kidney function. The PubMed, EMBASE, Cochrane Library, Scopus, and Web of Science databases were systematically searched from 1995 to 1 June, 2020, for clinical studies on circulating TMAO concentrations and kidney function indicators. We used R software to conduct meta-analyses of the extracted data. A cumulative meta-analysis was applied to test whether health status affected the pooled effect value. Meta-regression and subgroup analyses were performed to identify possible sources of heterogeneity. Ultimately, we included a total of 32 eligible clinical studies involving 42,062 participants. In meta-analyses of continuous-outcome variables, advanced CKD was associated with a 67.9 μmol/L (95% CI: 52.7, 83.2; P < 0.01) increase in TMAO concentration, and subjects with high concentrations of TMAO had a 12.9 mL/(min·1.73 m2) (95% CI: -16.6, -9.14; P < 0.01) decrease in glomerular filtration rate (GFR). In meta-analyses of the correlations, TMAO was strongly inversely correlated with GFR [Fisher's z-transformed correlation coefficient (ZCOR): -0.45; 95% CI: -0.58, -0.32; P < 0.01] and positively associated with the urine albumin-to-creatinine ratio (UACR; ZCOR: 0.26; 95% CI: 0.08, 0.43; P < 0.01), serum creatinine (sCr; ZCOR: 0.43; 95% CI: 0.28, 0.58; P < 0.01), urine albumin excretion rate (UAER; ZCOR: 0.06; 95% CI: 0.04, 0.09; P < 0.01), blood urea (ZCOR: 0.50; 95% CI: 0.29, 0.72; P < 0.01), blood uric acid (ZCOR: 0.32; 95% CI: 0.25, 0.38; P < 0.01), and serum cystatin C (CysC; ZCOR: 0.47, 95% CI: 0.44, 0.51; P < 0.01). This is the first systematic review and meta-analysis to reveal a negative association between circulating TMAO concentrations and kidney function.
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http://dx.doi.org/10.1093/advances/nmab010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321840PMC
July 2021

Genome-Wide Analysis Reveals the Potential Role of MYB Transcription Factors in Floral Scent Formation in .

Front Plant Sci 2021 26;12:623742. Epub 2021 Feb 26.

The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China.

The MYB gene family is one of the largest groups of transcription factors (TFs) playing diverse roles in several biological processes. (white ginger lily) is a renowned ornamental plant both in tropical and subtropical regions due to its flower shape and strong floral scent mainly composed of terpenes and benzenoids. However, there is no information available regarding the role of the MYB gene family in . In the current study, the MYB gene family was identified and extensively analyzed. The identified 253 genes were unevenly mapped on 17 chromosomes at a different density. Promoter sequence analysis showed numerous phytohormones related to -regulatory elements. The majority of genes contain two to three introns and motif composition analysis showed their functional conservation. Phylogenetic analysis revealed that HcMYBs could be classified into 15 distinct clades, and the segmental duplication events played an essential role in the expansion of the gene family. Tissue-specific expression patterns of genes displayed spatial and temporal expression. Furthermore, seven () were selected for further investigation. Through RT-qPCR, the response of candidates genes toward jasmonic acid methyl ester (MeJA), abscisic acid (ABA), ethylene, and auxin was examined. Yeast one-hybrid (Y1H) assays revealed that candidate genes directly bind to the promoter of bottom structural volatile synthesis genes (, , , and ). Moreover, yeast two-hybrid (Y2H) assay showed that HcMYB7/8/75/145/248 interact with HcJAZ1 protein. In -silenced flowers, the floral volatile contents were decreased and downregulated the expression of key structural genes, suggesting that these genes might play crucial roles in floral scent formation in by regulating the expression of floral scent biosynthesis genes. Collectively, these findings indicate that genes might be involved in the regulatory mechanism of terpenoids and benzenoid biosynthesis in .
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http://dx.doi.org/10.3389/fpls.2021.623742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952619PMC
February 2021

Exosomal miR‑663b exposed to TGF‑β1 promotes cervical cancer metastasis and epithelial‑mesenchymal transition by targeting MGAT3.

Oncol Rep 2021 04 2;45(4). Epub 2021 Mar 2.

Department of Obstetrics and Gynecology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.

Transforming growth factor (TGF)‑β1 is a key cytokine affecting the pathogenesis and progression of cervical cancer. Tumor‑derived exosomes contain microRNAs (miRNAs/miRs) that interact with cancer and stromal cells, thereby contributing to tissue remodeling in the tumor microenvironment (TME). The present study was designed to clarify how TGF‑β1 affects tumor biological functions through exosomes released by cervical cancer cells. Deep RNA sequencing found that TGF‑β1 stimulated cervical cancer cells to secrete more miR‑663b‑containing exosomes, which could be transferred into new target cells to promote metastasis. Further studies have shown that miR‑663b directly targets the 3'-untranslated regions (3'‑UTR) of mannoside acetylglucosaminyltransferase 3 (MGAT3) and is involved in the epithelial‑mesenchymal transition (EMT) process. Remarkably, the overexpression of MGAT3 suppressed cervical cancer cell metastasis promoted by exosomal miR‑663b, causing increased expression of epithelial differentiation marker E‑cadherin and decreased expression of mesenchymal markers N‑cadherin and β‑catenin. Throughout our study, online bioinformation tools and dual luciferase reporter assay were applied to identify as a novel direct target of miR‑663b. Exosome PKH67‑labeling experiment verified that exosomal miR‑663b could be endocytosed by cervical cancer cells and subsequently influence its migration and invasion functions which were measured by wound healing and Transwell assays. The expression of miR‑663b and MGAT3 and the regulation of the EMT pathway caused by MGAT3 were detected by quantitative real‑time transcription‑polymerase chain reaction (qPCR) and western blot analysis. These results, thus, provide evidence that cancer cell‑derived exosomal miR‑663b is endocytosed by cervical cancer cells adjacent or distant after TGF‑β1 exposure and inhibits the expression of MGAT3, thereby accelerating the EMT process and ultimately promoting local and distant metastasis.
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http://dx.doi.org/10.3892/or.2021.7963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877003PMC
April 2021

Effectively removing tetracycline from water by nanoarchitectured carbons derived from CO: Structure and surface chemistry influence.

Environ Res 2021 04 16;195:110883. Epub 2021 Feb 16.

School of Resource and Environmental Science, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Wuhan University, Wuhan, 430072, China; State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan, 430072, PR China. Electronic address:

Understanding of the correlation between physico-chemical property of adsorbent and the adsorption performance of contaminant is very significant for developing high-efficient materials to remove antibiotic contamination from water. In this work, a novel kind of carbon adsorbent (EC) derived from CO and activated ECs with modified structure via a facile chemical method using H and KOH were prepared. The synthetic carbon materials (EC, EC-H, and EC-KOH) were then applied to remove tetracycline (TC). The kinetics of adsorption for these three carbon materials all well fitted the pseudo-second-order kinetic model. The experimental data of adsorption isotherm had good compatibility with Langmuir and Freundlich models (R > 0.90), but the Temkin model was the most applicable for all adsorbents (R > 0.98). A super-high adsorption capacity of EC-KOH obtained from Langmuir fitting was 933.56 mg g, which was much higher than that of EC-H (538.91 mg g) and EC (423.30 mg g), possibly due to its larger specific surface area (S), pore volume, and specific surface chemical structure. Moreover, it was found that surface functional groups and large aperture of adsorbents had a positive effect on adsorption rate. More adsorption sites and surface functional groups of adsorbents were beneficial to enhance the adsorption affinity. These results are of great benefit to the directional control of carbon structure to increase the adsorption performance in rate, capacity, and affinity of antibiotics.
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http://dx.doi.org/10.1016/j.envres.2021.110883DOI Listing
April 2021
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