Publications by authors named "Xinyue Bai"

9 Publications

  • Page 1 of 1

Validation and optimization of key biochar properties through iron modification for improving the methane oxidation capacity of landfill cover soil.

Sci Total Environ 2021 Jun 19;793:148551. Epub 2021 Jun 19.

Shenzhen Engineering Laboratory for Eco-efficient Recycled Materials, School of Environment and Energy, Peking University Shenzhen Graduate School, University Town, Xili, Nanshan District, Shenzhen 518055, PR China. Electronic address:

Knowledge of various BC properties in stimulating the methane (CH) oxidation capacity of landfill cover soil (LCS) is still limited, restricting the optimization of BC performance. To validate key BC properties and seek a feasible way for enhancing BC performance, this study prepared BCs with distinctly varying characteristics through iron (Fe) modification. Then, batch incubation experiments under different CH and oxygen concentrations were conducted. Pore volume, cation exchange capacity (CEC), and surface area of BC collectively accounted for 78.5% of the variances in the microbial community structures, with pore volume being the most important factor. These correlated well with the differences in the CH oxidation capacities among the different BC-amended LCS. At a low ratio of 15% (v/v) in LCS, BCs' pH not affected their performance but homogeneity could be a limiting factor. Fe modification proved a promising approach to more efficiently improve the three key BC properties (especially pore volume) and thus optimize BC performance than increasing pyrolysis temperature did. Fe-modified BCs encouraged a bacterial consortium (methanotroph, methylotrophs, and nitrogen-fixing bacteria) in the soil with significantly improved CH oxidation capacities by up to 26%-74% compared to that of pristine BC.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148551DOI Listing
June 2021

Modeling Cone/Cone-Rod Dystrophy Pathology by AAV-Mediated Overexpression of Mutant CRX Protein in the Mouse Retina.

Transl Vis Sci Technol 2021 06;10(7):25

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Purpose: This study aims to evaluate the pathogenesis of cone/cone-rod dystrophy (CoD/CoRD) caused by a cone-rod homeobox (CRX) mutation, which was identified in a Chinese family, through adeno-associated virus (AAV)-mediated overexpression of mutant CRX protein in the mouse retina.

Methods: Comprehensive ophthalmologic examinations were performed for the pedigree members of a Chinese family with CoD/CoRD. Whole exome sequencing and Sanger sequencing were performed to determine the genetic cause of the disease. Furthermore, AAV vectors were used to construct AAV-CRX-mut-HA, which was transfected into mouse photoreceptor cells to clarify the pathogenesis of the mutant CRX.

Results: Fundus photography and optical coherence tomography images displayed features that were consistent with CoD/CoRD, including macular atrophy and photoreceptor layer thinning. Electroretinogram analysis indicated an obvious decrease in photopic responses or both scotopic and photopic responses in affected individuals. A frameshift variant c.611delC (p.S204fs) in CRX was cosegregated with the disease in this family. AAV-CRX-mut-HA that subretinally injected into the C57BL/6 mice generally transfected the outer nuclear layer, leading to the loss of cone and rod photoreceptor cells, abnormal expression of CRX target genes, and a decrease in electroretinogram responses.

Conclusions: AAV-mediated overexpression of CRX[S204fs] in the mouse retina led to a CoRD-like phenotype and showed the possible pathogenesis of the antimorphic CRX mutation.

Translational Relevance: This study provides a modeling method to evaluate the pathogenesis of CoD/CoRD and other inherited retinal dystrophies caused by distinct gain-of-function mutations.
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http://dx.doi.org/10.1167/tvst.10.7.25DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237110PMC
June 2021

FifBase: a comprehensive fertility-associated indicators factor database for domestic animals.

Brief Bioinform 2021 Jan 26. Epub 2021 Jan 26.

College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.

Fertility refers to the ability of animals to maintain reproductive function and give birth to offspring, which is an important indicator to measure the productivity of animals. Fertility is affected by many factors, among which environmental factors may also play key roles. During the past years, substantial research studies have been conducted to detect the factors related to fecundity, including genetic factors and environmental factors. However, the identified genes associated with fertility from countless previous studies are randomly dispersed in the literature, whereas some other novel fertility-related genes are needed to detect from omics-based datasets. Here, we constructed a fertility index factor database FifBase based on manually curated published literature and RNA-Seq datasets. During the construction of the literature group, we obtained 3301 articles related to fecundity for 13 species from PubMed, involving 2823 genes, which are related to 75 fecundity indicators or 47 environmental factors. Eventually, 1558 genes associated with fertility were filtered in 10 species, of which 1088 and 470 were from RNA-Seq datasets and text mining data, respectively, involving 2910 fertility-gene pairs and 58 fertility-environmental factors. All these data were cataloged into FifBase (http://www.nwsuaflmz.com/FifBase/), where the fertility-related factor information, including gene annotation and environmental factors, can be browsed, retrieved and downloaded with the user-friendly interface.
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http://dx.doi.org/10.1093/bib/bbaa432DOI Listing
January 2021

Multimodal imaging features and genetic findings in Bietti crystalline dystrophy.

BMC Ophthalmol 2020 Aug 15;20(1):331. Epub 2020 Aug 15.

Department of Ophthalmology and Vision Science, the Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China.

Background: Bietti crystalline dystrophy (BCD) is a distinct entity of retinitis pigmentosa with a wide range of genotypic and phenotypic variabilities. The goal of the present study was to investigate the morphological, functional and genetic features of BCD.

Methods: A full series of multimodal imaging was performed in four Chinese patients with BCD, including fundus photography, fundus autofluorescence, fundus fluorescein angiography (FFA), indocyanine green (ICG) angiography, optical coherence tomography (OCT) and microperimetry. Electrophysiological tests including full-field electroretinography (ERG) and multifocal ERG were employed. CYP4V2 gene sequencing was performed.

Results: Intraretinal crystalline deposits were observed in fundus photographs in all patients. The crystals were better appreciated in infrared images. Autofluorescence imaging demonstrated multifocal patchy hypofluorescence, suggesting massive RPE atrophy. FFA and ICG angiography further confirmed atrophy of the RPE and the underlying choroidal vessels. OCT revealed disruption of the photoreceptors, RPE and the choroid. Outer retinal tubulations (ORTs) confining to the outer nuclear layer were detected in three out of four patients. Full-field ERG showed markedly diminished responses. Multifocal ERG displayed reduced central and peripheral responses in a patient with normal vision. Gene sequencing identified two deletion mutations in CYP4V2, c.802_807del and c.810delT. BCD complicated by choroidal neovascularization (CNV) was diagnosed in one patient, and intravitreal anti-vascular endothelial growth factor (VEGF) injection was given with favorable response.

Conclusions: Multimodal imaging features and electrophysiological findings of BCD patients were comprehensively discussed. A novel deletion mutation, c.802_807del, in the CYP4V2 gene was reported. ORTs are important changes in the outer retina of BCD patients, further investigation of this structure may provide insights into pathology of BCD. Intravitreal anti-VEGF therapy was effective for treatment of BCD complicated by CNV.
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http://dx.doi.org/10.1186/s12886-020-01545-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429681PMC
August 2020

Generation of an integration-free induced pluripotent stem cell line (FDEENTi003-A) from a patient with pathological myopia.

Stem Cell Res 2019 08 9;39:101495. Epub 2019 Jul 9.

Department of Ophthalmology & Vision Science, Eye & ENT Hospital, Shanghai Medical School, Fudan University, Shanghai, China; Key NHC Key Laboratory of Myopia (Fudan University), Laboratory of Myopia, Chinese Academy of Medical Sciences, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Eye & ENT Hospital, Shanghai Medical School, Fudan University, Shanghai, China. Electronic address:

Pathological myopia (PM) is a major cause of irreversible vision impairment worldwide. We have successfully reprogrammed the peripheral blood mononuclear cells (PBMCs) from a PM patient to induced pluripotent stem cells and characterized their pluripotency and genetic stability, as well as the potential to differentiate to retinal pigment epithelium (RPE). This line may serve as a useful tool to explore the pathogenesis of PM.
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http://dx.doi.org/10.1016/j.scr.2019.101495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077977PMC
August 2019

Establishment of an induced pluripotent stem cell line (FDEENTi002-A) from a patient with Best's disease carrying c.888C > A mutation in BEST1 gene.

Stem Cell Res 2019 07 9;38:101459. Epub 2019 May 9.

Department of Ophthalmology & Vision Science, Eye & ENT Hospital, Shanghai Medical School, Fudan University, Shanghai, China; Key NHC Key Laboratory of Myopia (Fudan University), Laboratory of Myopia, Chinese Academy of Medical Sciences, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Eye & ENT Hospital, Shanghai Medical School, Fudan University, Shanghai, China. Electronic address:

Best's disease (BD) is an inherited retinal degenerative disease caused by mutations in BEST1 gene. A human induced pluripotent stem cell (iPSC) line has been generated with integration-free Sendai virus method from peripheral blood mononuclear cells (PBMCs) of a BD patient carrying c.888C > A mutation in BEST1 gene. This cell line may serve as a model for the study of pathogenesis of BD.
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http://dx.doi.org/10.1016/j.scr.2019.101459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156156PMC
July 2019

Establishment of an induced pluripotent stem cell line (FDEENTi001-A) from a patient with pathological myopia.

Stem Cell Res 2019 01 14;34:101369. Epub 2018 Dec 14.

Department of Ophthalmology & Vision Science, Eye & ENT Hospital, Shanghai Medical School, Fudan University, Shanghai, China; Key NHC Key Laboratory of Myopia (Fudan University), Laboratory of Myopia, Chinese Academy of Medical Sciences, China. Electronic address:

Pathological myopia (PM) is a retinal degenerative disease with an increasing prevalence in Asia. The peripheral blood mononuclear cells (PBMCs) from a patient with PM were successfully reprogrammed to induced pluripotent stem cells (iPSCs) using integration-free method, Sendai viral (SeV) vectors expressing OCT4, SOX2, KLF4 and C-MYC. This line may provide a useful resource for exploring the pathogenesis of PM.
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http://dx.doi.org/10.1016/j.scr.2018.101369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441353PMC
January 2019

Infection after Acupoint Embedding Therapy.

Plast Reconstr Surg Glob Open 2017 Sep 26;5(9):e1471. Epub 2017 Sep 26.

Department of Burns and Plastic Surgery, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing, P.R. China; and Department of Burns and Plastic Surgery, Chinese PLA Medical School, Beijing, P.R. China.

Nontuberculous mycobacterium is a ubiquitous environmental organism that is unusual to cause a true infection, but it can cause severe cutaneous infections. In this case report, we present a successful treatment for a Chinese patient with cutaneous infection after acupoint embedding therapy. We managed to conduct pathogenic detection, drug sensitive test, and multidisciplinary consultation. Finally, a systematic treatment strategy of nontuberculous mycobacterium was performed. Twenty-two-month follow-up revealed excellent outcome without any recurrence.
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http://dx.doi.org/10.1097/GOX.0000000000001471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640348PMC
September 2017
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