Publications by authors named "Xinyi Liu"

282 Publications

Obtaining Parallel Sentences in Low-Resource Language Pairs with Minimal Supervision.

Comput Intell Neurosci 2022 3;2022:5296946. Epub 2022 Aug 3.

Control Engineering College, Chengdu University of Information Technology, Chengdu 610225, China.

Machine translation relies on parallel sentences, the number of which is an important factor affecting the performance of machine translation systems, especially in low-resource languages. Recent advances in learning cross-lingual word representations from nonparallel data by machine learning make a new possibility for obtaining bilingual sentences with minimal supervision in low-resource languages. In this paper, we introduce a novel methodology to obtain parallel sentences via only a small-size bilingual seed lexicon about hundreds of entries. We first obtain bilingual semantic by establishing cross-lingual mapping in monolingual languages via a seed lexicon. Then, we construct a deep learning classifier to extract bilingual parallel sentences. We demonstrate the effectiveness of our methodology by harvesting Uyghur-Chinese parallel sentences and constructing a machine translation system. The experiments indicate that our method can obtain large and high-accuracy bilingual parallel sentences in low-resource language pairs.
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http://dx.doi.org/10.1155/2022/5296946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365574PMC
August 2022

Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry.

Front Vet Sci 2022 26;9:923238. Epub 2022 Jul 26.

Key Lab of Animal Epidemiology and Zoonoses of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China.

Fumonisin B1 (FB1) is a major fusarium mycotoxin that largely contaminates feedstuffs and foods, posing a health risk to both animals and humans. This mycotoxin can enter the human body directly through contaminated food consumption or indirectly by toxins and their metabolites. In a prior study, feed-borne FB1 is one of the leading mycotoxins in breeder eggs, leading to reduced hatchability and gizzard ulceration in chicken progenies. Currently, no effective way is available to remove FB1 from feeds and human-contaminated foods. We hypothesize that FB1 can be reduced to low risk by protocatechuic acid (PCA). To assess the ability of FB1 to be degraded , 1 ppm of FB1 was treated with PCA, or D-glucose, or silymarin, or anti-FB1 monoclonal antibody. Our study revealed that both D-glucose and PCA exhibited 53.4 and 71.43% degradation, respectively, at 80°C for 2 h, while 35.15% of FB1 detoxification was determined in the silymarin group at 60°C for 0.5 h. A dose-dependent manner was found after treatment with D-glucose or PCA at 80°C for 2 h. As for detoxification of anti-FB1 monoclonal antibody, the 1:3,000 dilution induced significant FB1 detoxification, accounting for 25.9% degradation at 25°C for 2 h. Furthermore, 50 SPF 11-day-old embryonated eggs were divided into 10 groups, with five eggs per group. Post treatment with PCA or D-glucose, or silymarin or anti-FB1 monoclonal antibody, the treated samples were inoculated into albumens and monitored daily until the hatching day. Consequently, 100% of the chickens survived in the D-glucose group and other control groups, except for the FB1 control group, while 80, 80, and 60% hatching rates were found in the PCA-treated group, the anti-FB1 monoclonal antibody-treated group, and the silymarin-treated group. Additionally, both the FB1 group and the silymarin-treated group yielded lower embryo growth than other groups did. Postmortem, lower gizzard ulceration index was determined in the PCA-treated group and the anti-FB1 monoclonal antibody-treated group compared to those of the silymarin-treated group and D-glucose-treated group. Based on the above evidence, PCA is a promising detoxification to reduce FB1 contamination in the poultry industry, contributing to the eradication of mycotoxin residuals in the food chain and maintaining food security for human beings.
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http://dx.doi.org/10.3389/fvets.2022.923238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360745PMC
July 2022

Fibroblast-derived exosomal miRNA-133 promotes cardiomyocyte-like differentiation.

Acta Histochem 2022 Aug 2;124(6):151931. Epub 2022 Aug 2.

Henan Medical Key Laboratory of Arrhythmia, Zhengzhou No. 7 People's Hospital, Zhengzhou, Henan, 450016, PR China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang City, Henan 453003, PR China. Electronic address:

Objective: To investigate the role of exosomal miRNA-133 secreted by cardiac fibroblasts (CFs) in promoting cardiomyocyte differentiation.

Methods: Neonatal rat CFs were cultured in vitro, and the cultured CFs were divided into three groups as follows: induction, miRNA-133 high expression, and miRNA-133 inhibition. miRNA-133 was transfected into CFs with lentivirus as a vector. CFs were transfected with the miRNA-133 inhibitor, and the markers of cardiomyocyte were detected through immunofluorescence staining, Western blotting, and real-time quantitative polymerase chain reaction (qRT-PCR) at 3, 8, and 14 days, respectively. The expression levels of cardiac troponin T (cTnT) and cardiac actin (α-actin) were determined, and qRT-PCR was used to detect the expression of miRNA-133 in the fibroblast exosomes.

Results: CFs subjected to immunofluorescence staining expressed vimentin and discoid domain receptor 2. The exosomes secreted by CFs were observed as small vesicles of 30-100 nm via transmission electron microscopy, and Western blotting was used to detect exosome-specific protein CD63 and CD9 expression. The expression levels of cTnT, α-actin, and exosomal miRNA-133 secreted into the supernatant of the miRNA-133 high-expression group increased gradually at different time points and reached the highest level at 14 days. The expression levels of cTnT, α-actin, and exosome miRNA-133 in the miRNA-133 inhibition group were the lowest.

Conclusion: The exosomal miRNA-133, which is derived from CFs, can promote the differentiation of fibroblasts into cardiomyocyte-like cells.
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http://dx.doi.org/10.1016/j.acthis.2022.151931DOI Listing
August 2022

Effects of long-term supplementation of probiotics on cognitive function and emotion in temporal lobe epilepsy.

Front Neurol 2022 19;13:948599. Epub 2022 Jul 19.

Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Cognitive impairment and neuropsychiatric disorders are very common in patients with temporal lobe epilepsy (TLE). These comorbidities complicate the treatment of epilepsy and seriously affect the quality of life. So far, there is still no effective intervention to prevent the development of epilepsy-associated comorbidities. Gut dysbiosis has been recognized to be involved in the pathology of epilepsy development. Modulating gut microbiota by probiotics has shown an antiseizure effect on humans and animals with epilepsy. Whether this treatment strategy has a positive effect on epilepsy-associated comorbidities remains unclear. Therefore, this study aimed to objectively assess the effect of probiotics on cognitive function and neuropsychiatric performance of patients with TLE. Participants enrolled in an epilepsy clinic were randomly assigned to the probiotic and placebo groups. These two groups were treated with probiotics or placebo for 12 weeks, and then the cognitive function and psychological performance of participants were assessed. We enrolled 76 participants in this study, and 70 subjects were finally included in the study (35 in the probiotics group and 35 in the placebo group). Our results showed significant seizure reduction in patients with TLE treated with probiotics. No significant differences were observed on cognitive function (including intelligence and memory) between groups. For neuropsychiatric performances, supplementation of probiotics significantly decreased the Hamilton Anxiety Rating and Depression Scale scores and increased the 89-item Quality of Life in Epilepsy Inventory score in patients with TLE. In conclusion, probiotics have a positive impact on seizures control, and improve anxiety, depression, and quality of life in patients with TLE.
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http://dx.doi.org/10.3389/fneur.2022.948599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343833PMC
July 2022

Robust and Efficient Assessment of Potency (REAP) as a quantitative tool for dose-response curve estimation.

Elife 2022 Aug 3;11. Epub 2022 Aug 3.

Department of Biostatistics, University of Texas MD Anderson Cancer Center, Houston, United States.

The median-effect equation has been widely used to describe the dose-response relationship and identify compounds that activate or inhibit specific disease targets in contemporary drug discovery. However, the experimental data often contain extreme responses, which may significantly impair the estimation accuracy and impede valid quantitative assessment in the standard estimation procedure. To improve the quantitative estimation of the dose-response relationship, we introduce a novel approach based on robust beta regression. Substantive simulation studies under various scenarios demonstrate solid evidence that the proposed approach consistently provides robust estimation for the median-effect equation, particularly when there are extreme outcome observations. Moreover, simulation studies illustrate that the proposed approach also provides a narrower confidence interval, suggesting a higher power in statistical testing. Finally, to efficiently and conveniently perform common lab data analyses, we develop a freely accessible web-based analytic tool to facilitate the quantitative implementation of the proposed approach for the scientific community.
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http://dx.doi.org/10.7554/eLife.78634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348845PMC
August 2022

Enhanced bone regeneration PHA scaffolds coated with polydopamine-captured BMP2.

J Mater Chem B 2022 Aug 17;10(32):6214-6227. Epub 2022 Aug 17.

Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Tsinghua-Peking Center of Life Sciences, Beijing 100084, China.

The hierarchical three-dimensional (3D)-printing scaffolds based on microbial polyester poly(3-hydroxybutyrate--4-hydroxybutyrate) (P34HB) were designed and used for bone tissue engineering surface functionalization on 3D-printed (P34HB) scaffolds using polydopamine (PDA)-mediated recombinant human bone morphogenetic protein-2 (BMP2), leading to enhanced bone formation in a rat model with a calvarial critical-size bone defect. Taking advantage of the adhesive property of PDA under alkaline and aerobic conditions, osteogenic BMP2 was captured on the surface of PHA scaffolds, resulting in their enhanced osteogenic bioactivity, better stem cell adhesion and proliferation, and sustainable release of a bioactive substance over a period of 30 days. These contributed to notable differences in alkaline phosphatase (ALP) activity, mineralization, expressions of osteogenesis-related genes, as well as finally enhanced bone formation in rats. The functionalized 3D-printed P34HB scaffolds the PDA-mediated osteogenic activity were developed as a versatile platform for bone tissue regeneration.
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http://dx.doi.org/10.1039/d2tb01122kDOI Listing
August 2022

Uneven Index: A Digital Biomarker to Prompt Blepharitis Based on Deep Learning.

Front Physiol 2022 11;13:934821. Epub 2022 Jul 11.

School of Ophthalmology and Optometry, Eye Hospital Wenzhou Medical University, Wenzhou, China.

To evaluate ocular surface manifestations and morphological changes in meibomian glands (MGs) based on artificial intelligence (AI) analysis in patients with blepharitis. In this retrospective study, 115 subjects were enrolled, including 64 subjects with blepharitis and 51 subjects without blepharitis as control group. Morphological indexes were evaluated for height, width, tortuosity, MG density, total variation, and the three types of corrected total variation as Uneven indexes. There were no statistically significant differences in all MGs' average tortuosity and width between the two groups. The average height of all MGs and MG density were significantly lower in the blepharitis group than control group. The total variation and two types of Uneven indexes were significantly higher in the blepharitis group than in the control group. Especially the Uneven Index of total variation/MG density had an AUC of 0.822. And the sensitivity and specificity were 59.4% and 92.2%, respectively, at a cut-off value of 3971.667. In addition, blepharitis was associated with significantly lower meibum quality and expressibility, severe atrophy of MGs, a higher ocular surface disease index (OSDI), and more instability of the tear film. mites are strongly associated with morphological changes in the MGs and may cause uneven gland atrophy. Therefore, the novel characteristic parameter, the Uneven index, may serve as a digital biomarker to evaluate uneven atrophy of MGs and prompt blepharitis.
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http://dx.doi.org/10.3389/fphys.2022.934821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309610PMC
July 2022

Multiplexed reverse-transcriptase quantitative polymerase chain reaction using plasmonic nanoparticles for point-of-care COVID-19 diagnosis.

Nat Nanotechnol 2022 Jul 25. Epub 2022 Jul 25.

Department of Biomedical Engineering, Columbia University, New York, NY, USA.

Quantitative polymerase chain reaction (qPCR) offers the capabilities of real-time monitoring of amplified products, fast detection, and quantitation of infectious units, but poses technical hurdles for point-of-care miniaturization compared with end-point polymerase chain reaction. Here we demonstrate plasmonic thermocycling, in which rapid heating of the solution is achieved via infrared excitation of nanoparticles, successfully performing reverse-transcriptase qPCR (RT-qPCR) in a reaction vessel containing polymerase chain reaction chemistry, fluorescent probes and plasmonic nanoparticles. The method could rapidly detect SARS-CoV-2 RNA from human saliva and nasal specimens with 100% sensitivity and 100% specificity, as well as two distinct SARS-CoV-2 variants. The use of small optical components for both thermocycling and multiplexed fluorescence monitoring renders the instrument amenable to point-of-care use. Overall, this study demonstrates that plasmonic nanoparticles with compact optics can be used to achieve real-time and multiplexed RT-qPCR on clinical specimens, towards the goal of rapid and accurate molecular clinical diagnostics in decentralized settings.
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http://dx.doi.org/10.1038/s41565-022-01175-4DOI Listing
July 2022

Serum Ang-1/Ang-2 ratio may be a promising biomarker for evaluating severity of diabetic retinopathy.

Graefes Arch Clin Exp Ophthalmol 2022 Jul 15. Epub 2022 Jul 15.

Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China.

Purpose: To investigate the predictive role of serum angiopoietin-1 and angiopoietin-2 (Ang-1/Ang-2) in evaluating the severity of diabetic retinopathy (DR).

Methods: A total of 101 outpatients with type 2 diabetes mellitus (T2DM) were recruited and were further divided into the following five groups: T2DM without DR (non-DR), mild non-proliferative DR (NPDR), moderate NPDR, severe NPDR and proliferative DR (PDR) in accordance with the International Clinical Diabetic Retinopathy Guidelines. Furthermore, 101 serum samples were included in the further analysis using enzyme-linked immunosorbent assays. A receiver operating characteristic (ROC) curve was plotted to evaluate the diagnostic value of each index.

Results: The expression of Ang-1 in the PDR group was significantly lower than that in the non-DR group, while Ang-2 showed an opposite upward trend (p < 0.05). The Ang-1/Ang-2 ratio of the non-DR group was significantly lower than that of the moderate NPDR, severe NPDR and PDR (p < 0.05, p < 0.01 and p < 0.01, respectively). Differences in the Ang-1/Ang-2 ratio were observed earlier than those in the individual Ang-1 and Ang-2 measurements. The maximal Youden index was 0.512 with a calculated area under the curve (AUC) value of 0.734 (p < 0.01).

Conclusions: The Ang-1/Ang-2 ratio was helpful in assessing the severity of DR and may provide potential clinical benefits as a biomarker and early warning signs for DR diagnosis.
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http://dx.doi.org/10.1007/s00417-022-05745-zDOI Listing
July 2022

Multi-target tracheloside and doxorubicin combined treatment of lung adenocarcinoma.

Biomed Pharmacother 2022 Jul 11;153:113392. Epub 2022 Jul 11.

School of Life Sciences, Hebei University, Baoding 071002, Hebei, China. Electronic address:

Chemotherapy is one of the main methods for malignant lung cancer treatment. However, the side effects of chemotherapy drugs are serious and it is prone to drug resistance. Therefore, multi-drug combination chemotherapy is popular in lung cancer treatment. This study found that tracheloside (TCS) was a novel inhibitor of TMEM16A which was specific high expressed in lung cancer tissues. TCS concentration dependently inhibited TMEM16A with an IC of 3.09 ± 0.21 μM. It inhibited lung cancer cells proliferation, migration, and induced cells apoptosis targeting TMEM16A. In addition, molecular docking combined with site-directed mutagenesis confirmed that the binding sites of TCS to TMEM16A were S387, E623, E624. Subsequently, multi-target combined drug administration was conducted based on the different drug targets of TCS and doxorubicin (DOX). Both in vitro and in vivo experiments indicated that the combined administration of low concentration of TCS and DOX achieved satisfactory anticancer effect, and it offset the side effects caused by high concentration of DOX. Therefore, TCS is a safe and efficient anticancer lead compound which can enhance the effect of DOX.
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http://dx.doi.org/10.1016/j.biopha.2022.113392DOI Listing
July 2022

Anomalous Origin of the Right Pulmonary Artery from the Aorta.

Authors:
Lin Zhou Xinyi Liu

Radiology 2022 Jul 12:220310. Epub 2022 Jul 12.

From the Department of Radiology, Third Affiliated Hospital of Chengdu Medical College & Pidu District People's Hospital, Chengdu, China (L.Z.); and Department of Radiology, Shenzhen Children's Hospital, Yitian Rd 4017, Shenzhen 518034, China (X.L.).

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http://dx.doi.org/10.1148/radiol.220310DOI Listing
July 2022

Caffeic Acid, an Active Ingredient in Coffee, Combines with DOX for Multitarget Combination Therapy of Lung Cancer.

J Agric Food Chem 2022 Jul 30;70(27):8326-8337. Epub 2022 Jun 30.

School of Life Sciences, Hebei University, Baoding 071002, Hebei, China.

Adjuvant diet therapy is an important means of comprehensive treatment of cancer. It is recognized by patients for its high safety, painlessness, and ease to operate. However, the development of adjuvant dietary therapy is limited by unclear targets and unclear anticancer mechanisms. In this work, caffeic acid was found as an inhibitor of TMEM16A with an IC of 29.47 ± 3.19 μM by fluorescence quenching and whole-cell patch-clamp experiments. Caffeic acid regulated the proliferation, migration, and apoptosis of lung cancer cells targeting TMEM16A, which was detected by CCK-8, colony formation, wound healing, and Annexin V assays. In addition, molecular docking combined with site-directed mutagenesis confirmed that the binding sites of caffeic acid to TMEM16A were D439, E448, and R753. Western blot results indicated that caffeic acid regulated the growth of lung cancer through the MAPK pathway. experiments showed that the inhibitory effect of caffeic acid combined with hydroxydaunorubicin (DOX) on lung cancer cell growth was better than a double concentration of any single dose. pharmacokinetic experiments and tumor xenograft experiments indicated that the combination of 5.4 mg/kg caffeic acid and 4.1 mg/kg DOX achieved 85.6% tumor suppression rate and offset the side effects. Therefore, caffeic acid is a safe and efficient antitumor active ingredient of food that can enhance the antitumor effect of DOX.
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http://dx.doi.org/10.1021/acs.jafc.2c03009DOI Listing
July 2022

CTCF organizes inter-A compartment interactions through RYBP-dependent phase separation.

Cell Res 2022 Aug 29;32(8):744-760. Epub 2022 Jun 29.

RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong, China.

Chromatin is spatially organized into three-dimensional structures at different levels including A/B compartments, topologically associating domains and loops. The canonical CTCF-mediated loop extrusion model can explain the formation of loops. However, the organization mechanisms underlying long-range chromatin interactions such as interactions between A-A compartments are still poorly understood. Here we show that different from the canonical loop extrusion model, RYBP-mediated phase separation of CTCF organizes inter-A compartment interactions. Based on this model, we designed and verified an induced CTCF phase separation system in embryonic stem cells (ESCs), which facilitated inter-A compartment interactions, improved self-renewal of ESCs and inhibited their differentiation toward neural progenitor cells. These findings support a novel and non-canonical role of CTCF in organizing long-range chromatin interactions via phase separation.
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http://dx.doi.org/10.1038/s41422-022-00676-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343660PMC
August 2022

Understanding the Stability of LiNiMnO as a Co-Free Positive Electrode.

J Phys Chem Lett 2022 Jul 29;13(26):6181-6186. Epub 2022 Jun 29.

School of Mechanical Engineering, Chengdu University, Chengdu, Sichuan 610106, People's Republic of China.

To understand the stability of Co-free positive electrode materials, LiNiMnO was synthesized with different amounts of lithium added during calcination. The valence states of the Ni and Mn transition metals of the prepared samples were determined through accurate stoichiometry analyses (via inductively coupled plasma optical emission spectrometry), magnetic moment measurements (via superconducting quantum interference device magnetometry), and element valence analyses (via X-ray spectroscopy in combination with Ar ion etching for depth profiling). Unexpectedly, the Ni and Mn transition metals in the interior and on the surface of the LiNiMnO particles show different electrochemical properties. This clarifies the open questions on the Li deintercalation mechanism in LiNiMnO.
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http://dx.doi.org/10.1021/acs.jpclett.2c01126DOI Listing
July 2022

Authors' response to "Comment on Si et al. (2021) 'Predictive performance of 7 frailty instruments for short-term disability, falls and hospitalization among Chinese community-dwelling older adults: A prospective cohort study'".

Int J Nurs Stud 2022 09 31;133:104302. Epub 2022 May 31.

School of Nursing, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing 100191, China. Electronic address:

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http://dx.doi.org/10.1016/j.ijnurstu.2022.104302DOI Listing
September 2022

Nirvana: A Qualitative Study of Posttraumatic Growth in Adolescents and Young Adults with Inflammatory Bowel Disease.

Children (Basel) 2022 Jun 13;9(6). Epub 2022 Jun 13.

School of Nursing, Yangzhou University, Yangzhou 225009, China.

(1) Background: Psychosomatic discomfort is prevalent among adolescents and young adults (AYAs) with inflammatory bowel disease (IBD). Post-traumatic growth (PTG) has been a protective factor in earlier research. However, little is known regarding PTG among AYAs with IBD. This study investigates the generation of PTG in adolescents and young adults with inflammatory bowel disease (IBD) and finds positive coping skills employed in clinical nursing practice. (2) Methods: In 2021, 32 individuals were interviewed utilizing a semi-structured interview guide. This study used qualitative content analysis. (3) Results: The interviews revealed five themes: spiritual change, internalized supportiveness, cognitive re-shaping, externalized behaviors, and future-oriented thinking. (4) Conclusions: The research revealed the presence of PTG in AYAs with IBD. To give tailored care to patients, medical professionals must monitor the state of their PTG development in a planned and focused manner.
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http://dx.doi.org/10.3390/children9060879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222066PMC
June 2022

NF-κB over-activation portends improved outcomes in HPV-associated head and neck cancer.

Oncotarget 2022 24;13:707-722. Epub 2022 May 24.

Department of Otolaryngology/Head and Neck Surgery, UNC, Chapel Hill, NC 27599, USA.

Evolving understanding of head and neck squamous cell carcinoma (HNSCC) is leading to more specific diagnostic disease classifications. Among HNSCC caused by the human papilloma virus (HPV), tumors harboring defects in are associated with improved clinical outcomes and maintenance of episomal HPV. TRAF3 and CYLD are negative regulators of NF-κB and inactivating mutations of either leads to NF-κB overactivity. Here, we developed and validated a gene expression classifier separating HPV+ HNSCCs based on NF-κB activity. As expected, the novel classifier is strongly enriched in NF-κB targets leading us to name it the NF-κB Activity Classifier (NAC). High NF-κB activity correlated with improved survival in two independent cohorts. Using NAC, tumors with high NF-κB activity but lacking defects in or were identified; thus, while gene defects identify the majority of tumors with NF-κB activation, unknown mechanisms leading to NF-kB activity also exist. The NAC correctly classified the functional consequences of two novel missense mutations. Using a reporter assay, we tested these CYLD mutations revealing that their activity to inhibit NF-kB was equivalent to the wild-type protein. Future applications of the NF-κB Activity Classifier may be to identify HPV+ HNSCC patients with better or worse survival with implications for treatment strategies.
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http://dx.doi.org/10.18632/oncotarget.28232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131933PMC
May 2022

Identification and Functional Analyses of Host Proteins Interacting with the p17 Protein of Avian Reovirus.

Viruses 2022 04 25;14(5). Epub 2022 Apr 25.

College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Avian reovirus (ARV) causes viral arthritis, chronic respiratory diseases, retarded growth and malabsorption syndrome. However, the precise molecular mechanism remains unclear. Here, we report the host cellular proteins that interact with ARV p17 by yeast two-hybrid screening. In this study, the p17 gene was cloned into pGBKT7 to obtain the bait plasmid pGBKT7-p17. After several rounds of screening of a chicken cDNA library, 43 positive clones were identified as possible host factors that interacted with p17. A BLAST search of the sequences was performed on the NCBI website, which ultimately revealed 19 interacting proteins. Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genome analyses indicated that the acquired proteins were involved in multicellular organismal processes, metabolic processes, and biological regulation. When the subcellular localization of the host protein and ARV p17 protein was investigated, we observed colocalization of p17-GFP with IGF2BP1-RED and PQBP1-RED in the transfected cells but not with FGF1-RED. The direct interaction of ARV p17 protein with IGF2BP1 and PQBP1 was confirmed by coimmunoprecipitation and GST pulldown assays. We used RT-qPCR to assess the expression variation during ARV infection. The results showed that IGF2BP1, PAPSS2, RPL5, NEDD4L, PRPS2 and IFI16 were significantly upregulated, whereas the expression of FGF1, CDH2 and PQBP1 was markedly decreased in DF-1 cells infected with ARV. Finally, we demonstrated that IGF2BP1 had a positive effect on ARV replication, while PQBP1 had the opposite effect. Our findings provide valuable information for better insights into ARV's pathogenesis and the role of the p17 protein in this process.
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http://dx.doi.org/10.3390/v14050892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145365PMC
April 2022

A biomimetic sensor using neurotransmitter detection to decode odor perception by an olfactory network.

Biosens Bioelectron 2022 Sep 18;211:114391. Epub 2022 May 18.

Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, China. Electronic address:

Mammalian olfactory perception is an important physiological function for tasks such as finding food, identifying species, and avoiding enemies. Previous studies have demonstrated the molecular basis of receptors and the anatomical structure of the olfactory bulb (OB), which is the initial processing center for odor perception. However, there remains some controversy about the coding and transmission mode of olfactory information in the OB through neuronal interaction mediated by different neurotransmitters. In this paper, a biomimetic sensor based on OB neuronal network was developed to detect trace amounts of typical neurotransmitters and decode odor perception in vitro. Primary OB neurons were seeded on a microelectrode array (MEA) chip to capture multichannel extracellular electrical activities. The firing features of OB neurons were statistically analyzed after stimulation with graded concentrations of the glutamate and gamma-aminobutyric acid (GABA). Cross-correlation analysis between channels was used to evaluate the connection status in the neuronal networks. The concentration-dependent responses to these two neurotransmitters were assessed over a range of values, and the lower limits of detection for glutamate and GABA were 100 nM and 50 nM, respectively. Stimulation with excessive concentrations produced response tolerance and neurotoxicity, and transmission between cells in the neural network was modulated by different neurotransmitters. This device could serve as a novel biosensor for detecting trace amounts of common neurotransmitters and for screening the effects of drugs on the physiology of olfaction. The response patterns of this biomimetic sensor are conducive to revealing the coding mechanism of information processing in the olfactory system.
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http://dx.doi.org/10.1016/j.bios.2022.114391DOI Listing
September 2022

Machine Learning-Based Radiomics for Prediction of Epidermal Growth Factor Receptor Mutations in Lung Adenocarcinoma.

Dis Markers 2022 7;2022:2056837. Epub 2022 May 7.

Department of Respiratory, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Institute of Respiratory Diseases, Shandong Institute of Anesthesia and Respiratory Critical Medicine, Jinan 250000, China.

Identifying an epidermal growth factor receptor (EGFR) mutation is important because EGFR tyrosine kinase inhibitors are the first-line treatment of choice for patients with EGFR mutation-positive lung adenocarcinomas (LUAC). This study is aimed at developing and validating a radiomics-based machine learning (ML) approach to identify EGFR mutations in patients with LUAC. We retrospectively collected data from 201 patients with positive EGFR mutation LUAC (140 in the training cohort and 61 in the validation cohort). We extracted 1316 radiomics features from preprocessed CT images and selected 14 radiomics features and 1 clinical feature which were most relevant to mutations through filter method. Subsequently, we built models using 7 ML approaches and established the receiver operating characteristic (ROC) curve to assess the discriminating performance of these models. In terms of predicting EGFR mutation, the model derived from radiomics features and combined models (radiomics features and relevant clinical factors) had an AUC of 0.79 (95% confidence interval (CI): 0.77-0.82), 0.86 (0.87-0.88), respectively. Our study offers a radiomics-based ML model using filter methods to detect the EGFR mutation in patients with LUAC. This convenient and low-cost method may be of help to noninvasively identify patients before obtaining tumor sample for molecule testing.
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http://dx.doi.org/10.1155/2022/2056837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107363PMC
May 2022

Responses of WJL-G4 upon Citric Acid Stress.

Molecules 2022 Apr 21;27(9). Epub 2022 Apr 21.

School of Forestry, Northeast Forestry University, No. 26, Hexing Street, Harbin 150040, China.

This study aimed to elucidate the responses of a novel characterized WJL-G4 against citric acid stress by performing physiological analysis, morphology observation, and structural and membrane fatty acid composition analysis. The results showed that under citric acid stress, the cell vitality of WJL-G4 was reduced. The cell morphology changed with the unclear, uncompleted and thinner cell wall, and degraded the cell structure. When the citric acid concentration was 20 g/L, WJL-G4 could tolerate citric acid and maintain the cell structure by increasing the intracellular pH, superoxide dismutase activity, and contents of unsaturated fatty acids. As the citric acid concentration was ≥80 g/L, the stress has exceeded the cellular anti-stress ability, causing substantial cell damage. The cell membrane permeability, the content of membrane lipids, malondialdehyde and superoxide anion increased, but the intracellular pH and superoxide dismutase activities decreased, accompanying the increase of citric acid concentrations. The findings of this work provided a theoretical basis for the responsive mechanism of WJL-G4 under high concentrations of citric acid, and can serve as a reference for biological acid reduction in fruit processing.
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http://dx.doi.org/10.3390/molecules27092664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102369PMC
April 2022

Estrogen and BRCA1 deficiency synergistically induce breast cancer mutation-related DNA damage.

Biochem Biophys Res Commun 2022 07 2;613:140-145. Epub 2022 May 2.

Vaccine Research Institute, The Third Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangzhou, 510630, China; Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510630, China; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China; West China Biomedical Big Data Center, West China Hospital, Sichuan University, Chengdu, 610041, China. Electronic address:

Estrogen (E2) is crucial for the development of breast cancer caused by BRCA1 mutation, and can increase the DNA damage in BRCA1-deficient cells. However, the mechanisms through which BRCA1 deficiency and E2 synergistically induce DNA damage remains unclear. In this study, we analyzed the distribution of DNA damage in E2-treated BRCA1-deficient cells. We detected DNA lesions in the vicinity of genes that are transcriptionally activated by estrogen receptor-α (ER). Loss of BRCA1 altered chromatin binding by ER, which significantly affected the distribution of DNA damage. Moreover, these changes were associated with the established mutations in BRCA1-mutant breast cancer. Taken together, our findings reveal a new mechanism underlying the DNA damage in breast cancer cells that is synergistically induced by BRCA1 deficiency and E2.
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http://dx.doi.org/10.1016/j.bbrc.2022.04.142DOI Listing
July 2022

Opsin1 regulates light-evoked avoidance behavior in Aedes albopictus.

BMC Biol 2022 05 13;20(1):110. Epub 2022 May 13.

Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China.

Background: Mosquitoes locate a human host by integrating various sensory cues including odor, thermo, and vision. However, their innate light preference and its genetic basis that may predict the spatial distribution of mosquitoes, a prerequisite to encounter a potential host and initiate host-seeking behaviors, remains elusive.

Results: Here, we first studied mosquito visual features and surprisingly uncovered that both diurnal (Aedes aegypti and Aedes albopictus) and nocturnal (Culex quinquefasciatus) mosquitoes significantly avoided stronger light when given choices. With consistent results from multiple assays, we found that such negative phototaxis maintained throughout development to adult stages. Notably, female mosquitoes significantly preferred to bite hosts in a shaded versus illuminated area. Furthermore, silencing Opsin1, a G protein-coupled receptor that is most enriched in compound eyes, abolished light-evoked avoidance behavior of Aedes albopictus and attenuated photonegative behavior in Aedes aegypti. Finally, we found that field-collected Aedes albopictus also prefers darker area in an Opsin1-dependent manner.

Conclusions: This study reveals that mosquitoes consistently prefer darker environment and identifies the first example of a visual molecule that modulates mosquito photobehavior.
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http://dx.doi.org/10.1186/s12915-022-01308-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103082PMC
May 2022

Interleukin‑27 ameliorates allergic asthma by alleviating the lung Th2 inflammatory environment.

Int J Mol Med 2022 Jun 6;49(6). Epub 2022 May 6.

Department of Respiratory Medicine, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Shandong Institute of Respiratory Diseases, Shandong Institute of Anesthesia and Respiratory Critical Medicine, Jinan, Shandong 250014, P.R. China.

Interleukin (IL)‑27 can inhibit the differentiation of Th2 cells and plays a role in the development of asthma. However, whether the therapeutic administration of IL‑27 in a mouse model of asthma can inhibit allergic responses remains a matter of debate. Additionally, the mechanisms through which IL‑27 ameliorates inflammatory responses in asthma are not yet fully understood. Thus, the aim of the present study was to examine the effects of IL‑27 on asthma using a mouse model and to elucidate the underlying mechanisms. For this purpose, mice received an intranasal administration of IL‑27 and the total and differential cell counts, levels of cytokines and type 1 regulatory T (Tr1) cells in the lungs were detected. The protein and mRNA levels of signal transducer and activator of transcription (STAT)1 and STAT3 were analyzed and airway remodeling was assessed. The results indicated that IL‑27 did not ameliorate airway inflammation, airway hyperresponsiveness, and airway remolding when administrated therapeutically. Preventatively, the administration of IL‑27 decreased the concentrations of Th2 cytokines and increased the number of Tr1 cells. The protein and mRNA levels of STAT1 and STAT3 were increased. Taken together, these findings demonstrate that the prophylactic administration of IL‑27 ameliorates asthma by alleviating the lung Th2 inflammatory environment through the restoration of both the STAT1 and STAT3 pathways. IL‑27 may thus prove to be useful as a novel agent for the prevention of asthma.
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http://dx.doi.org/10.3892/ijmm.2022.5142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106376PMC
June 2022

Genome-Wide Screening and Characterization of Genes Involved in Response to High Dose of Ciprofloxacin in .

Microb Drug Resist 2022 May 18;28(5):501-510. Epub 2022 Feb 18.

Department of Microbiology, Harbin Medical University, Harbin, China.

The global emergence of antibiotic resistance, especially in Gram-negative bacteria, is an urgent threat to public health. Inevitably, considering its extensive use and misuse, resistance toward ciprofloxacin has increased in almost all clinically relevant bacteria. This study aimed to investigate the transcriptome changes at a high concentration of ciprofloxacin in . In brief, 1,418 differentially expressed genes (DEGs) were identified, from which 773 genes were upregulated by ciprofloxacin, whereas 651 genes were downregulated. Enriched biological pathways reflected the upregulation of biological processes such as DNA damage and repair system, toxin/antitoxin systems, formaldehyde detoxification system. With kyoto encyclopedia of genes and genomes pathway analysis, higher expressed DEGs were associated with "LPS biosynthesis," "streptomycin biosynthesis," and "polyketide sugar unit biosynthesis." Lower expressed DEGs were associated with "biosynthesis of amino acids" and "flagellar assembly" pathways. After treatment of ciprofloxacin, lipopolysaccharide (LPS) release was increased by two times, and the gene expression level of LPS synthesis was elevated ( < 0.05) in both reference and clinical strains. Our results demonstrated that transient exposure to high-dose ciprofloxacin is a double-edged sword. Cautions should be taken when administering high-dose antibiotic treatment for infectious diseases.
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http://dx.doi.org/10.1089/mdr.2021.0117DOI Listing
May 2022

Efficacy of MP-3 microperimeter biofeedback fixation training for low vision rehabilitation in patients with maculopathy.

BMC Ophthalmol 2022 Apr 28;22(1):197. Epub 2022 Apr 28.

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University, 100 Haining Road, Hongkou District, Shanghai, 200080, China.

Background: To evaluate the efficacy of MP-3 microperimeter biofeedback fixation training (MBFT) in vision rehabilitation of low-vision patients affected by macular disease with central vision loss.

Methods: Seventeen eyes (7 age-related macular degeneration, 10 myopic maculopathy) of 17 patients were included in this prospective, interventional study. The preferred retinal locus was determined by comprehensive ophthalmoscopic fundus evaluation including fundus photography, autofluorescence, optical coherence tomography, and microperimetry. The rehabilitation consisted of three 10-min sessions per eye to be performed twice per week for 20 consecutive weeks using the MP-3 microperimeter. Best corrected visual acuity (BCVA), reading speed, mean central sensitivity, the percentages of fixation points within specified regions, bivariate contour ellipse area (BCEA) and the 25-item National Eye Institute visual function questionnaire (NEI-VFQ-25) were recorded pre- and post-training.

Results: The final BCVA, reading speed and mean central sensitivity all showed significant improvements after rehabilitation (P <  0.0001, P = 0.0013, and P = 0.0002, respectively). The percentages of fixation points located within 2° and 4° diameter circles both significantly increased after training (P = 0.0008 and P = 0.0007, respectively). The BCEA encompassing 68.2, 95.4, 99.6% of fixation points were all significantly decreased after training (P = 0.0038, P = 0.0022, and P = 0.0021, respectively). The NEI-VFQ-25 scores were significantly increased at the end of the rehabilitation training (P <  0.0001).

Conclusion: Rehabilitation with MP-3 MBFT is a user-friendly therapeutic option for improving visual function, fixation stability, and quality of life in advanced macular disease.

Trial Registration: The prospective study was registered with the Chinese Clinical Trial Registry ( http://www.chictr.org.cn/ ).

Trial Registration Number: ChiCTR2000029586 . Date of registration: 05/02/2020.
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http://dx.doi.org/10.1186/s12886-022-02419-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047472PMC
April 2022

Primary hepatic mucosa-associated lymphoid tissue lymphoma with HP and previous HBV infection: A case report and literature review.

J Infect Chemother 2022 Aug 19;28(8):1182-1188. Epub 2022 Apr 19.

Department of Haematology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, 225000, China. Electronic address:

Primary hepatic mucosa-associated lymphoid tissue (MALT) lymphoma is a relatively rare disease with low malignancy, and its aetiology is unclear. A 65-year-old man presented with abdominal pain. Hepatitis virus examination revealed a previous hepatitis B virus (HBV) infection, and a carbon-13 urea breath test result was positive for the patient. Abdominal contrast-enhanced computed tomography revealed a patch of abnormal density in the right posterior lobe of the liver. The patient underwent VI segment hepatectomy and was pathologically diagnosed with hepatic MALT lymphoma. After the operation, he received quadruple anti-Helicobacter pylori (HP) therapy and refused other treatments. He has been followed up by telephone for 20 months after discharge and is now in a stable condition. In this study, we counted 105 cases of hepatic MALT lymphomas reported in English or Chinese since 1995 and summarised the clinical characteristics and concomitant diseases in this condition. Based on the literature review, we speculated that chronic infectious diseases, especially viral infections (including hepatitis C virus (HCV) and HBV) and HP infection, are associated with the pathogenesis of primary hepatic MALT lymphoma. In addition, autoimmune diseases might also play a role in this condition.
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http://dx.doi.org/10.1016/j.jiac.2022.04.014DOI Listing
August 2022

Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride Nanosphere.

Biosensors (Basel) 2022 Apr 9;12(4). Epub 2022 Apr 9.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Hollow carbon nitride nanosphere (HCNS) was synthesized via the hard template method to improve the fluorescence characteristics, drug delivery ability, and photocatalytic activity. Blue fluorescent HCNS was utilized as a quenching agent and an internal reference to combine with Cy5-labelled aptamer (Cy5-Apt), resulting in an off-on fluorescence aptasensing method for the detection of Salmonella typhimurium (). Under optimum conditions, this fluorescence assay presented a linear range from 30 to 3 × 10 CFU mL with a detection limit of 13 CFU mL. In addition, HCNS was also used as a drug carrier to load chloramphenicol (Cap) molecules. The Cap-loading amount of HCNS could reach 550 μg mg within 24 h, whereas the corresponding Cap-release amount is 302.5 μg mg under acidic and irradiation conditions. The integration of photocatalyst with antibiotic could endow HCNS-Cap with better disinfection performance. The bactericidal efficiency of HCNS-Cap (95.0%) against within 12 h was better than those of HCNS (85.1%) and Cap (72.9%). In addition, selective disinfection of was further realized by decorating aptamer. Within 4 h, almost all were inactivated by HCNS-Cap-Apt, whereas only 13.3% and 48.2% of and cells were killed, respectively. Therefore, HCNS is a promising bio-platform for aptamer-based fluorescence detection and selective disinfection of .
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http://dx.doi.org/10.3390/bios12040228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027708PMC
April 2022

Factors associated with benefit finding and mental health of patients with cancer: a systematic review.

Support Care Cancer 2022 Aug 7;30(8):6483-6496. Epub 2022 Apr 7.

School of Nursing, Yangzhou University, Hanjing County, 136 Jiangyang Middle Road, Yangzhou City, 225009, Jiangsu Province, China.

Purpose: This systematic review was performed to identify the association between benefit finding (BF) and anxiety, depression, and distress, as well as to find factors related to BF in adult cancer patients.

Methods: Five English databases and four Chinese databases were searched to identify eligible articles about BF in adult cancer patients from inception to 4 July 2021. Literature screening, data extraction, and quality assessment were carried out by two reviewers independently according to the inclusion and exclusion criteria. Qualitative synthesis was used to summarize the results.

Results: Twenty-one studies involving 9601 cancer patients were included in this study. Qualitative analysis showed that BF was negatively related to anxiety, depression, and distress. Factors associated with BF included sociodemographic factors, disease-related factors, and psychological and environmental factors.

Conclusions: Healthcare providers should pay attention to the summarized factors of BF and construct intervention programs based on the controllable factors to improve BF in adult cancer patients, thus promoting the recovery of patients.
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http://dx.doi.org/10.1007/s00520-022-07032-3DOI Listing
August 2022

Profiles and transition of mental health problems among Chinese adolescents: The predictive role of friendship quality, parental autonomy support, and psychological control.

J Adolesc 2022 Jan 26;94(1):19-33. Epub 2022 Jan 26.

Shanghai Key Laboratory of Mental Health and Psychological Crisis Intervention, Institute of Brain and Education Innovation, School of Psychology and Cognitive Science, East China Normal University, Shanghai, China.

Introduction: Mental health problems have become a global crisis of the 21st century, with adolescence being a typical period of the outbreak of these problems. However, the profiles and transition of mental health problems in Chinese adolescents remain unclear. In addition, protective and risk factors that shape mental health problems require further clarification.

Methods: We measured depression, anxiety, and stress as indicators to identify the profiles and transition patterns of mental health problems among Chinese adolescents, as well as environment-related predictors (i.e., friendship quality, parental psychological control, and autonomy support). A total of 722 participants (376 females; M  = 15.21, SD  = 0.74) completed a set of questionnaires at two time points with a 1-year interval (T1: November 2018; T2: November 2019).

Results: The profile analysis revealed two groups: healthy and troubled. The development of mental health problems included four trajectories: steady low, steady high, increasing, and decreasing. Results indicated that parental autonomy support and friendship quality exerted protective and buffering effects, whereas parental psychological control acted as a risk factor for mental health problem profiles. Furthermore, friendship quality had a unique predictive effect on the decreasing trajectory.

Conclusions: The profiles of mental health problems showed high concurrency of depression, anxiety, and stress symptoms, and developmental trajectories were largely stable over time. Friendship quality, parental autonomy support, and psychological control predicted the profiles of the mental health problems of Chinese adolescents, and only friendship quality predicted the transition from a troubled to healthy profile over 1 year.
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http://dx.doi.org/10.1002/jad.12002DOI Listing
January 2022
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