Publications by authors named "Xinxin Xu"

121 Publications

Hydrophobic POM Electrocatalyst Achieves Low Voltage "Charge" in Zn-air Battery Coupled with Bisphenol A Degradation.

Chemistry 2021 Apr 12. Epub 2021 Apr 12.

inorganic institution, chemistry department, CHINA.

Zn-air battery is a perspective power source for grid-storage. But, after they are discharged at 1.1 to 1.2 V, large overpotential is required for their charging, usually 2.5 V. This is due to sluggish the oxygen evolution reaction (OER); Incorporating organic pollutants into the cathode electrolyte is a feasible strategy for lowering the required charging potential. On the other hand, in discharge process related oxygen reduction reaction, hydrophobic electrocatalyst is more popular than hydrophilic one. Here, a hydrophobic bi-functional polyoxometalate electrocatalyst is synthesized by precise structural design. It shows excellent activities in both bisphenol A degradation and oxygen reduction reactions. In bisphenol A containing electrolyte, to achieve 100 mA·cm -2 , its potential is only 1.32 V, which is 0.34 V lower than oxygen evolution reaction. In oxygen reduction reaction, this electrocatalyst follows four-electron mechanism. In both bisphenol A degradation and oxygen reduction reactions, it shows excellent stability. With this electrocatalyst as cathode material and bisphenol A containing KOH as electrolyte, Zn-air battery is assembled. When "charge" at 85 mA·cm -2 , it only requires 1.98 V. Peak power density of this Zn-air battery reaches 120.5 mW·cm -2 . More importantly, in "charge" process, bisphenol A is degraded, which achieves energy saving and pollutants removal simultaneously in one Zn-air battery.
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http://dx.doi.org/10.1002/chem.202100412DOI Listing
April 2021

Lateral flow immunoassay for the simultaneous detection of fipronil and its metabolites in food samples.

Food Chem 2021 Mar 27;356:129710. Epub 2021 Mar 27.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

We developed a sensitive and rapid lateral flow immunochromatographic (LFI) assay for the simultaneous detection of fipronil and its metabolites in eggs and cucumbers using gold nanoparticle (GNP)-labeled monoclonal antibodies (mAbs). Anti-fipronil mAbs (1B6) were produced using two haptens and identified by heterologous indirect competitive enzyme-linked immunosorbent assay (icELISA) with half maximal inhibitory concentration (IC) and limit of detection (LOD) values of 0.46 ± 0.07 and 0.05 ± 0.01 ng mL, respectively. The developed LFI strip showed high sensitivity and specificity in the detection of fipronil with cut-off and visual limit of detection (vLOD) values of 10 and 0.25 ng mL, respectively. Furthermore, the application of LFI in the detection of fipronil-spiked egg and cucumber samples was validated by liquid chromatography tandem mass spectrometry (LC-MS/MS). Our developed LFI assay is suitable for detection of fipronil and its metabolites in real samples.
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http://dx.doi.org/10.1016/j.foodchem.2021.129710DOI Listing
March 2021

Gold nanoparticle-based immunochromatographic assay for detection in water and food samples.

Food Chem X 2021 Mar 5;9:100117. Epub 2021 Mar 5.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

() is the common infection-causing bacterial pathogen. Conventional methods for the detection of are time-consuming, and therefore, a more rapid analytical method is required. Here, monoclonal antibodies (Mabs) against (CICC 10419) were prepared and based on paired Mabs, an immunochromatographic assay (ICA) was developed. The ICA strip showed a limit of detection of 2.41 × 10 CFU/mL and the linear range of detection was 3.13 × 10-1.0 × 10 CFU/mL. No cross-reactivity was observed when other common Gram-negative and Gram-positive bacteria were used. The analytical performance of the ICA strip indicated that the developed ICA had good specificity and stability. Moreover, the feasibility of the ICA strip was verified by detecting (CICC 10419) in spiked water and food samples. The ICA strip could detect samples contaminated with a low-level of (CICC 10419) after 8 h enrichment.
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http://dx.doi.org/10.1016/j.fochx.2021.100117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985707PMC
March 2021

Disc1 gene down-regulation impaired synaptic plasticity and recognition memory via disrupting neural activity in mice.

Brain Res Bull 2021 Mar 18;171:84-90. Epub 2021 Mar 18.

College of Life Sciences and Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, 300071, Tianjin, PR China. Electronic address:

The gene of Disrupted-in-schizophrenia 1 (Disc1) is closely related to mental diseases with cognitive deficits, but there are few studies on the changes in neural oscillations and recognition memory. Neural oscillations plays a key role in the nervous system in a dynamic form, which is closely related to advanced cognitive activities such as information processing and memory consolidation. Hence, we aimed to investigate if Disc1 knockdown disrupted the normal pattern of neural activities in the mouse hippocampus network, and determined if quantitative neural oscillation approach could be a potential diagnostic tool for mental disorders. In the study, we reported that Disc1 gene, downregulated by short-hairpin RNA (shRNA), not only induced anxiety-like behavior and sociability impairment but also damaged both synaptic plasticity and recognition memory in mice. Moreover, Disc1 knockdown mice exhibited evidently abnormal power spectral distributions, reduced phase synchronizations, and decreased phase-amplitude coupling strength compared to that of normal animals. In addition, transcriptome analyses showed that there were clearly transcriptional changes in Disc1 knockdown mice. Altogether, our findings suggest that the abnormal pattern of neural activities in the hippocampus network disrupts information processing and finally leads to the impairments of synaptic plasticity and recognition in Disc1 knockdown mice, which are possibly associated with the obstruction of neurotransmitter transmission. Importantly, the data imply that the analysis of neural oscillation pattern provides a potential diagnosis approach for mental disorders.
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http://dx.doi.org/10.1016/j.brainresbull.2021.03.011DOI Listing
March 2021

Prognostic significance of poorly differentiated histology and impact of adjuvant chemotherapy in early squamous cell carcinoma of cervix uteri.

Cancer Med 2021 Apr 18;10(8):2611-2617. Epub 2021 Mar 18.

Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.

Objective: This study is to determine whether the addition of cisplatin-based chemotherapy after radical hysterectomy will improve the survival of low-risk squamous cervical carcinoma with poor differentiation.

Methods: Patients with low-risk squamous cervical cancer (FIGO IA2-IIA, absent high- and intermediate-risk factors after pathological evaluation) were eligible for this study. As first, the prognostic relevance of G3 versus G1/G2 among patients with low-risk squamous cervical cancer was analyzed, then, the oncological results of postoperative chemotherapy among low-risk squamous cervical cancer with poor differentiation was explored.

Results: Totally, there were 367 low-risk squamous cervical cancer patients, of whom 161 were poor-differentiated (47 in the chemotherapy group and 114 in the nonchemotherapy group), with a median follow-up time of 56 months. Patients with G3 displayed a significantly worse overall survival (p = 0.035), and a higher recurrence rate (p = 0.014) than patients with G1/G2. Compared with the nonchemotherapy group, the hazard ratios (95%CI) for recurrence-free survival in the chemotherapy group was 0.24 (0.06-0.93), (p = 0.038). No difference in overall survival was observed between the chemotherapy group and the nonchemotherapy group.

Conclusions: The addition of cisplatin-based chemotherapy following surgery significantly improved recurrence-free survival for low-risk, poor differentiation, and early stage squamous cervical cancer patients.
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http://dx.doi.org/10.1002/cam4.3780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026924PMC
April 2021

Activation of liver X receptors prevents emotional and cognitive dysfunction by suppressing microglial M1-polarization and restoring synaptic plasticity in the hippocampus of mice.

Brain Behav Immun 2021 Mar 2. Epub 2021 Mar 2.

College of Life Sciences and Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, 300071 Tianjin, PR China. Electronic address:

Depression is a long-lasting and persistent mood disorder in which the regulatory mechanisms of neuroinflammation are thought to play a contributing role to the physiopathology of the condition. Previous studies have shown that liver X receptors (LXRs) can regulate the activation of microglia and neuroinflammation. However, the role of LXRs in depression remains to be fully understood. In this study, we hypothesized that stress impairs the function of LXRs and that the LXRs agonist GW3965 plays a potential anti-depressive role by inhibiting neuroinflammation. The anti-depressive effects of GW3965 were evaluated in both chronic unpredictable mild stress (CUMS) and lipopolysaccharide (LPS) models. The LXRs antagonist GSK2033 was also employed to block LXRs. Behavioural tests were performed to measure depression-like phenotypes and learning abilities. Electrophysiological recordings and Golgi staining were used to measure the plasticity of the dentate gyrus synapse. The expression of synapse and neuroinflammation related proteins were evaluated by Western blotting and immunofluorescence. The activation of LXRs by GW3965 prevented emotional and cognitive deficits induced by either CUMS or LPS. GW3965 prevented the decreased level of LXR-β induced by CUMS. The activation of LXRs significantly improved the impairment of synaptic plasticity, prevented the up-regulation of inflammatory factors and inhibited NF-κB phosphorylation and microglial M1-polarization in both models. The antidepressive-like effects of GW3965 were blocked by GSK2033 in the CUMS and LPS models. Our data suggest that inhibition of the LXRs signalling pathway may be a key driver in the pathogenesis of neuroinflammation during depression and that LXRs agonists have a high potential in the treatment of depression.
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http://dx.doi.org/10.1016/j.bbi.2021.02.026DOI Listing
March 2021

The Prevalence and Risk Factors of Cerebral Microbleeds: A Community-Based Study in China.

Ther Clin Risk Manag 2021 18;17:165-171. Epub 2021 Feb 18.

Department of Neurology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.

Introduction: Cerebral microbleeds (CMBs) are frequently found in the healthy elderly. However, data on the prevalence and risk factors of CMBs in the general population of China are lacking.

Methods: A cross-sectional study focusing on the prevalence and risk factors of CMBs was conducted in stroke-free elderly from Shanghai Wuliqiao community. MRI was performed at 3Tesla and cardiovascular risk factors (eg, age, smoking history, and hypertension), cerebral small vessel disease (CSVD) markers (eg, white matter hyperintensities, lacunar infarction, and enlarged perivascular space) and genetic information (eg, APOE, CR1) were recorded. Poisson regression was used to analyze the risk factors of the presence and location of microbleeds.

Results: A total of 199 participants (70.8±7.2 years old; male 31.2%) were finally included in our analysis. The overall prevalence of CMBs was 12.6% (25/199) and increased with age from 7.5% (55-64 years old) to 19.3% (over 75 years old). Of those with CMBs, most of them (16/25) located in the deep/mixed region and had 1-2 CMBs (18/25). Poisson regression analysis showed that white matter hyperintensities (OR=1.22, 95% CI: 1.16-1.29), APOE ε4+ carrier (OR=2.16, 95% CI: 1.18-3.96) and CR1 non-F/F isoform (OR=7.78, 95% CI: 4.34-13.96) were associated with CMBs. Further analysis found that in addition to the above three risk factors, hypertension (OR=2.98, 95% CI: 1.16-7.64), lacunar infarction (OR=2.39, 95% CI: 1.19-4.81) also increased the risk of deep/mixed CMBs.

Conclusion: The prevalence of cerebral microbleeds is similar to other countries. Cardiovascular risk factors, CSVD markers, and genetic factors (APOE ε4, CR1 non-F/F isoform) were associated with CMBs, suggesting an interaction of multiple pathogenesis in Chinese stroke-free community population.
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http://dx.doi.org/10.2147/TCRM.S297708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917356PMC
February 2021

Discovery of A031 as effective proteolysis targeting chimera (PROTAC) androgen receptor (AR) degrader for the treatment of prostate cancer.

Eur J Med Chem 2021 Apr 23;216:113307. Epub 2021 Feb 23.

State Key Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, 222 S. Tianshui Rd, Lanzhou, 730000, PR China. Electronic address:

Androgen receptor (AR) is an effective therapeutic target for the treatment of prostate cancer. We report herein the design, synthesis, and biological evaluation of highly effective proteolysis targeting chimeras (PROTAC) androgen receptor (AR) degraders, such as compound A031. It could induce the degradation of AR protein in VCaP cell lines in a time-dependent manner, achieving the IC 50 value of less than 0.25 μM. The A031 is 5 times less toxic than EZLA and works with an appropriate half-life (t 1/2) or clearance rate (Cl). Also, it has a significant inhibitory effect on tumor growth in zebrafish transplanted with human prostate cancer (VCaP). Therefore, A031 provides a further idea of developing novel drugs for prostate cancer.
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http://dx.doi.org/10.1016/j.ejmech.2021.113307DOI Listing
April 2021

Effect of Postreinduction Therapy Consolidation With Blinatumomab vs Chemotherapy on Disease-Free Survival in Children, Adolescents, and Young Adults With First Relapse of B-Cell Acute Lymphoblastic Leukemia: A Randomized Clinical Trial.

JAMA 2021 03;325(9):833-842

Department of Pediatrics, Benioff Children's Hospital and the Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco.

Importance: Standard chemotherapy for first relapse of B-cell acute lymphoblastic leukemia (B-ALL) in children, adolescents, and young adults is associated with high rates of severe toxicities, subsequent relapse, and death, especially for patients with early relapse (high risk) or late relapse with residual disease after reinduction chemotherapy (intermediate risk). Blinatumomab, a bispecific CD3 to CD19 T cell-engaging antibody construct, is efficacious in relapsed/refractory B-ALL and has a favorable toxicity profile.

Objective: To determine whether substituting blinatumomab for intensive chemotherapy in consolidation therapy would improve survival in children, adolescents, and young adults with high- and intermediate-risk first relapse of B-ALL.

Design, Setting, And Participants: This trial was a randomized phase 3 clinical trial conducted by the Children's Oncology Group at 155 hospitals in the US, Canada, Australia, and New Zealand with enrollment from December 2014 to September 2019 and follow-up until September 30, 2020. Eligible patients included those aged 1 to 30 years with B-ALL first relapse, excluding those with Down syndrome, Philadelphia chromosome-positive ALL, prior hematopoietic stem cell transplant, or prior blinatumomab treatment (n = 669).

Interventions: All patients received a 4-week reinduction chemotherapy course, followed by randomized assignment to receive 2 cycles of blinatumomab (n = 105) or 2 cycles of multiagent chemotherapy (n = 103), each followed by transplant.

Main Outcome And Measures: The primary end point was disease-free survival and the secondary end point was overall survival, both from the time of randomization. The threshold for statistical significance was set at a 1-sided P <.025.

Results: Among 208 randomized patients (median age, 9 years; 97 [47%] females), 118 (57%) completed the randomized therapy. Randomization was terminated at the recommendation of the data and safety monitoring committee without meeting stopping rules for efficacy or futility; at that point, 80 of 131 planned events occurred. With 2.9 years of median follow-up, 2-year disease-free survival was 54.4% for the blinatumomab group vs 39.0% for the chemotherapy group (hazard ratio for disease progression or mortality, 0.70 [95% CI, 0.47-1.03]); 1-sided P = .03). Two-year overall survival was 71.3% for the blinatumomab group vs 58.4% for the chemotherapy group (hazard ratio for mortality, 0.62 [95% CI, 0.39-0.98]; 1-sided P = .02). Rates of notable serious adverse events included infection (15%), febrile neutropenia (5%), sepsis (2%), and mucositis (1%) for the blinatumomab group and infection (65%), febrile neutropenia (58%), sepsis (27%), and mucositis (28%) for the chemotherapy group.

Conclusions And Relevance: Among children, adolescents, and young adults with high- and intermediate-risk first relapse of B-ALL, postreinduction treatment with blinatumomab compared with chemotherapy, followed by transplant, did not result in a statistically significant difference in disease-free survival. However, study interpretation is limited by early termination with possible underpowering for the primary end point.

Trial Registration: ClinicalTrials.gov Identifier: NCT02101853.
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http://dx.doi.org/10.1001/jama.2021.0669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926290PMC
March 2021

Down-regulation of MST1 in hippocampus protects against stress-induced depression-like behaviours and synaptic plasticity impairments.

Brain Behav Immun 2021 Feb 17. Epub 2021 Feb 17.

College of Life Sciences and Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, 300071 Tianjin, PR China. Electronic address:

Depression is a common mental disorder, and its main environmental risk factor is chronic stress. The activation of mammalian STE20-like kinase 1 (MST1), a key factor involved in the underlying pathophysiology of stress, can trigger synaptic plasticity impairment, neuronal dysfunction and neuroinflammation. However, it is unclear whether down-regulation of MST1 in the hippocampus protects against stress-induced behavioural dysfunctions. In this study, three mouse models were used to assess the role of MST1 in stress. Various behavioural tests, in vivo electrophysiological recordings, Western blotting, Golgi staining and immunofluorescence assay were used. The data showed that the level of phospho-MST1 (T183) was significantly increased in the hippocampus of mice subjected to chronic unpredictable mild stress (CUMS) and that mice with MST1 overexpression showed depression-like behaviours. Importantly, the impairment of cognitive functions and the hippocampal synaptic plasticity induced by CUMS were significantly improved by MST1 knockdown, suggesting that MST1 down-regulation effectively protected against stress-induced behavioural dysfunctions. Moreover, MST1 knockdown suppressed CUMS-induced microglial activation, reduced the abnormal expression of inflammatory cytokines and impeded the activation of p38, implying that the antidepressant-like effects of MST1 knockdown were associated with inhibiting the p38 pathway. These findings suggest that hippocampal MST1 is an essential regulator of stress, which can be an ideal target for the development of antidepressants in the future.
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http://dx.doi.org/10.1016/j.bbi.2021.02.007DOI Listing
February 2021

Sensitive Lateral Flow Immunoassay for the Residues of Imidocarb in Milk and Beef Samples.

ACS Omega 2021 Feb 18;6(4):2559-2569. Epub 2021 Jan 18.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China.

Based on the successful derivation of a hapten, we prepared and optimized a murine monoclonal antibody against imidocarb, with an IC of 2.22 ng/mL and a limit of detection (LOD) of 0.45 ng/mL. Cross-experiment results showed that the cross-over rate for 4,4'-dinitrocarbanilide was 18.12%, and the cross-reactivity with other analogues when using the ic ELISA was less than 0.1%. We used the developed ic-ELISA to detect the addition and recovery of imidocarb in milk and beef samples, and values were 86.0-93.5 and 84.5-101.2%, respectively. The preparation of an immunochromatographic test strip based on gold nanoparticles was used for the rapid identification of imidocarb in milk and beef samples. When assessed by the naked eye, the visual LOD for imidocarb in milk and beef samples was 5 and 10 ng/mL, and the cut-off values were 20 and 50 ng/mL, respectively. Because of its high sensitivity, specificity, and simplicity, the test strip can be used for on-site testing and rapid screening of imidocarb in food samples.
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http://dx.doi.org/10.1021/acsomega.0c04422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859938PMC
February 2021

Immunoassays for the rapid detection of pantothenic acid in pharmaceutical and food products.

Food Chem 2021 Jun 18;348:129114. Epub 2021 Jan 18.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China.

Pharmaceutical and food products are fortified with pantothenic acid (PA) to address potential deficiency. Therefore, its fast, reliable, and accurate detection is of great importance to the quality control. Here, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a gold nanoparticle-based lateral flow immunoassay (LFIA) were established for the determination of PA based on an anti-PA monoclonal antibody (mAb). The ic-ELISA displayed a limit of detection (LOD) of 32.22 ng/mL, and the linear range was 64.44-628.84 ng/mL. Average recoveries of PA in fortified samples were 88.60-110.11% when using the ic-ELISA and a good correlation between the ic-ELISA and LC-MS/MS was obtained when analyzing samples. Furthermore, the developed LFIA strip showed a calculated LOD of 71.99, 115.80, and 240.12 ng/mL in B-complex Vitamin tablets, energy drink and infant milk powder samples, respectively. All the results demonstrated that both of these immunoassays are suitable for determining PA in pharmaceutical and food products.
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http://dx.doi.org/10.1016/j.foodchem.2021.129114DOI Listing
June 2021

Development of a monoclonal antibody-based immunochromatographic strip for the rapid detection of tigecycline in human serum.

Anal Methods 2021 02;13(6):817-824

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and was found to have a 50%-inhibitory concentration (IC50) of 2.303 ng mL-1 and a limit of detection (LOD) of 0.215 ng mL-1 for TGC. This strip assay had a visual limit of detection (vLOD) of 50 ng mL-1 and a cut-off value of 1000 ng mL-1 for TGC in human serum, when assessed by eye. With the aid of a strip scan reader, a quantitative determination of TGC was obtained with a calculated limit of detection (cLOD) of 15.03 ng mL-1. Analysis of TGC in human serum indicated that the results of our strip assay compared well with results obtained using ic-ELISA and LC-MS/MS. Therefore, this strip assay represents a sensitive and reliable method for the on-site detection of TGC in serum samples.
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http://dx.doi.org/10.1039/d0ay02182bDOI Listing
February 2021

Presenilin 1 mutation likely contributes to U1 small nuclear RNA dysregulation and Alzheimer's disease-like symptoms.

Neurobiol Aging 2021 Apr 6;100:1-10. Epub 2021 Jan 6.

College of Life Sciences & State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, PR China. Electronic address:

Previous studies showed that U1 small nuclear RNA (snRNA) was selectively enriched in the brain of individuals with familial Alzheimer's disease (AD), resulting in widespread changes in RNA splicing. Our study further reported that presenilin-1 (PSEN1) induced an increase in U1 snRNA expression, accompanied by changed amyloid precursor protein expression, β-amyloid level, and cell death in SH-SY5Y cells. However, the effect of U1 snRNA overexpression on learning and memory is still unclear. In the present study, we found that neuronal U1 snRNA overexpression could generate U1 snRNA aggregates in the nuclear, accompanied by the widespread alteration of RNA splicing, resulting in the impairments of synaptic plasticity and spatial memory. In addition, more U1 snRNAs is bound to the intron binding sites accompanied by an increased intracellular U1 snRNA level. This suggests that U1 snRNA overexpression regulates RNA splicing and gene expression in neurons by manipulating the recruitment of the U1 snRNA to the nascent transcripts. Using in situ hybridization staining of human central nervous system-type neurons, we identified nuclear aggregates of U1 snRNA in neurons by upregulating the U1 snRNA level. Quantitative polymerase chain reaction analysis showed U1 snRNA accumulation in the insoluble fraction of neurons with PSEN1 mutation neurons rather than other types of U snRNAs. These results show an independent function of U1 snRNA in regulating RNA splicing, suggesting that aberrant RNA processing may mediate neurodegeneration induced by PSEN1 mutation.
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http://dx.doi.org/10.1016/j.neurobiolaging.2020.12.015DOI Listing
April 2021

Recepteur d'origine nantais contributes to the development of endometriosis via promoting epithelial-mesenchymal transition of a endometrial epithelial cells.

J Cell Mol Med 2021 Feb 6;25(3):1601-1612. Epub 2021 Jan 6.

Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Endometriosis is a benign, chronic inflammatory disease that commonly occurs in reproductive-aged women. Epithelial-mesenchymal transition (EMT) of endometrial epithelial cells plays an important role in the development of endometriosis. Recepteur d'origine nantais (RON), a receptor tyrosine kinase, has been reported to promote EMT and progression in tumours. However, whether and how RON mediates the EMT and endometriosis development is not known. Here, we found that RON activation could improve the migratory and invasive capabilities, change cellular morphologies, and decrease expression of E-cadherin and increase expression of N-cadherin in endometrial epithelial cells. Inhibition or knockdown of RON expression suppressed the migration and invasion of endometrial epithelial cells. Our studies also indicated that RON played its part in endometrial epithelial cells through protein kinase B (Akt) and mitogen-activated protein kinase (MAPK) pathways. Treatment with a RON inhibitor could decrease the number of ectopic lesions in a mouse model of endometriosis and mediate expression of EMT markers in endometriotic lesions. These data suggest that RON contributed to endometriosis development by promoting EMT of endometrial epithelial cells. Therefore, RON may be a new therapeutic target for endometriosis.
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http://dx.doi.org/10.1111/jcmm.16261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875913PMC
February 2021

Better survival of right-sided than left-sided stage II colon cancer: a propensity scores matching analysis based on SEER database.

Turk J Gastroenterol 2020 11;31(11):805-813

Department of General surgery, The Forth Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

Background/aims: Most studies have found that right-sided colon cancer (RCC) has worse prognosis than left-sided colon cancer (LCC), especially in stage III, but the reported prognosis of stage II colon cancer is variable. This study aimed to evaluate the impact of tumor location on survival outcomes in stage II colon cancer.

Materials And Methods: Patients with stage II colon cancer were identified in the Surveillance, Epidemiology, and End Results database from 2004 to 2009. The effect of tumor location on overall survival and cancer-specific survival was analyzed using Cox proportional hazards regression models and propensity score matching.

Results: Of 16,519 patients, 69.6% had RCC and30.4% had LCC. In unadjusted analyses, RCC had a 13% increased overall mortality risk (hazards ratio [HR], 1.13; 95% confidence interval [CI], 1.07-1.19; p<0.001) but an18% reduction in cancer-specific mortality risk compared with LCC (HR, 0.82; 95% CI, 0.76-0.89; p<0.001). After propensity scores matching analyses, RCC had a 21% reduced overall mortality risk (HR, 0.79; 95% CI, 0.72-0.87; p<0.001) and a 49% reduction in cancer-specific mortality risk compared with LCC (HR, 0.51; 95% CI, 0.44-0.60; p<0.001).

Conclusion: When adjusted for multiple clinicopathological features, stage II RCC showed better prognosis than stage II LCC.
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http://dx.doi.org/10.5152/tjg.2020.19531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759218PMC
November 2020

Recognition and Removal of Amyloid-β by a Heteromultivalent Macrocyclic Coassembly: A Potential Strategy for the Treatment of Alzheimer's Disease.

Adv Mater 2021 Jan 16;33(4):e2006483. Epub 2020 Dec 16.

College of Life Sciences and Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin, 300071, P. R. China.

The imbalance of amyloid-β (Aβ) production and clearance causes aggregation of Aβ monomers to form fibrils and amyloid plaques, which is an indispensable process in the pathogenesis of Alzheimer's disease (AD), and eventually leads to pathological changes and cognitive impairment. Consequently, Aβ is the most important target for the treatment of AD. However, developing a single treatment method that can recognize Aβ , inhibit Aβ fibrillation, eliminate amyloid plaques, improve cognitive impairments, and alleviate AD-like pathology is challenging. Here, a coassembly composed of cyclodextrin (CD) and calixarene (CA) is designed, and it is used as an anti-Aβ therapy agent. The CD-CA coassembly is based on the previously reported heteromultivalent recognition strategy and is able to successfully eliminate amyloid plaques and degrade Aβ monomers in 5xFAD mice. More importantly, the coassembly improves recognition and spatial cognition deficits, and synaptic plasticity impairment in the 5xFAD mice. In addition, the coassembly ameliorates AD-like pathology including prevention of neuronal apoptosis and oxidant stress, and alteration of M1/M2 microglial polarization states. This supramolecular approach makes full use of both molecular recognition and self-assembly of macrocyclic amphiphiles, and is a promising novel strategy for AD treatment.
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http://dx.doi.org/10.1002/adma.202006483DOI Listing
January 2021

Surface Atom Regulation on Polyoxometalate Electrocatalyst for Simultaneous Low-Voltage H Production and Phenol Degradation.

ACS Appl Mater Interfaces 2020 Nov 17. Epub 2020 Nov 17.

Department of Chemistry, College of Science, Northeastern University, Shenyang 110819, China.

The electrocatalytic hydrogen evolution reaction is an ideal method for H production. To improve the performance of polyoxometalate-based electrocatalyst in the hydrogen evolution reaction, one O in polyoxometalate is replaced by S. This weakens the binding of polyoxometalate to H*, facilitates its desorption, and improves the H generation property. Vulcanized polyoxometalate only requires 55 mV to achieve 10 mA·cm current in the hydrogen evolution reaction. This electrocatalyst also exhibits promising performance in phenol degradation reaction, which is an ideal substitute for high-energy-consuming oxygen evolution reaction in H production due to low voltage to drive. To acquire 100 and 200 mA·cm in the phenol degradation reaction, this vulcanized polyoxometalate only consumes 1.38 and 1.41 V. With this electrocatalyst working as a cathode and an anode simultaneously, an electrolyzer is constructed by employing phenol-containing KOH as an electrolyte. To obtain 100 and 200 mA·cm current, the electrolyzer only requires 1.54 and 1.57 V. Because energy-efficient phenol degradation reaction occurs, these values are obviously lower than the oxygen evolution reaction involved in the overall water-splitting H production. This work provides a universal method to enhance the hydrogen evolution reaction (HER) activity of polyoxometalates. Furthermore, a new method is explored, which achieves energy conservation and phenol degradation simultaneously in H production.
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http://dx.doi.org/10.1021/acsami.0c14431DOI Listing
November 2020

Europium nanosphere-based fluorescence strip sensor for ultrasensitive and quantitative determination of fumonisin B.

Anal Methods 2020 11 21;12(43):5229-5235. Epub 2020 Oct 21.

State Key Laboratory of Food Science and Technology, Jiangnan University, China.

Contamination of grains and related products by fumonisins (FBs) is increasingly becoming a serious food security issue. The aim of this work was to develop a europium fluorescent microsphere-based time-resolved fluorescence immunochromatographic assay (TRFICA) for FB detection in different grains, including corn, corn flour, wheat, rice and brown rice. Standard curves for the five types of grain matrix were established, and showed good linearity (R > 0.975), LOD of 8.26 μg kg, and a wide working range of 13.81-1000 μg kg. The recoveries of TRFICA for FB detection ranged from 82.85-103.62% with variation coefficients of 1.92-15.33%. Two corn reference materials and other natural samples were tested using TRFICA. The same samples analyzed by liquid chromatography tandem mass spectrometry further confirmed the TRFICA results. The entire detection time of TRFICA was within 30 min. Thus, this developed TRFICA can be used for onsite detection and quantitation of FB in grains.
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http://dx.doi.org/10.1039/d0ay01734eDOI Listing
November 2020

Expanding the genetic and phenotypic spectrum of the subcortical maternal complex genes in recurrent preimplantation embryonic arrest.

Clin Genet 2021 Feb 14;99(2):286-291. Epub 2020 Oct 14.

Clinical Research Center for Reproduction and Genetics in Hunan Province, Reproductive and Genetic Hospital of CITIC-XIANGYA, Changsha, China.

The subcortical maternal complex (SCMC) is an oocyte-to-embryo-specific maternal functional module. Some variants of SCMC genes that contribute to preimplantation embryonic arrest have been identified. However, more novel variants should be identified to broaden the genetic and phenotypic spectrum of SCMC genes and establish their roles in embryonic development. We identified 13 novel variants in the SCMC genes, TLE6, NLRP5, NLRP2, and PADI6, from 10 of a total of 50 infertile females with recurrent preimplantation embryonic arrest. Six variants in TLE6 were found in five patients with embryonic arrest, accompanied by direct cleavage and severe fragmentation at the cleavage stage. Three patients carried NLRP5 variants, and one patient each who carried NLRP2 and PADI6 variants had subsequent poor or failed fertilization and cleavage arrest with a relatively lower ratio of severely fragmented embryos. Our findings expand the genetic and phenotypic spectrum of SCMC genes associated with human embryogenesis and might help lay the foundation for the genetic diagnosis of female infertility.
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http://dx.doi.org/10.1111/cge.13858DOI Listing
February 2021

Development of signature-tagged mutagenesis in Riemerella anatipestifer to identify genes essential for survival and pathogenesis.

Vet Microbiol 2020 Nov 19;250:108857. Epub 2020 Sep 19.

Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai 200241, China. Electronic address:

Riemerella anatipestifer causes epizootic infectious disease in ducks, geese, turkeys and other birds, and serious economic losses especially to the duck industry. However, little is known about the molecular basis of its pathogenesis. In this study, signature-tagged transposon mutagenesis based on Tn4351 was developed in R. anatipestifer to identify genes essential for survival and pathogenesis. Seventeen tagged Tn4351 random mutation libraries of the R. anatipestifer strain WJ4 containing 5100 mutants were screened for survive using a duckling infection model. Twenty mutants that could not be recovered from the infected ducklings, were identified, and 17 mutated genes were identified by inverse PCR or genome-walking PCR. Of these genes, FIP52_03215, FIP52_04350 and FIP52_09345, were inserted into two mutant strains, and FIP52_03215 and FIP52_03175 were found exclusively on the chromosome of serotype 1 R. anatipestifer strains. Twelve out of 17 genes encoding for proteins were predicted to be involved in amino acid, nucleotide, coenzyme, or lipid transport and metabolism, one gene was predicted to be involved in signal transduction, one gene was predicted to be involved in DNA replication, recombination and repair, the other three genes had an unknown function. Animal experiments showed that the virulence of mutants 16-284, 7-295, 24-231, 9-232 and 19-214 were significantly attenuated compared to that of the wild-type WJ4. Moreover, the median lethal dose of mutant 16-284 was greater than 10 CFU, and its virulence to ducklings was partially restored when it was complemented with the shuttle expression plasmid pRES-FIP52_09345. The results in this study will be helpful to further study the molecular mechanisms of the pathogenesis of R. anatipestifer infection.
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http://dx.doi.org/10.1016/j.vetmic.2020.108857DOI Listing
November 2020

Whole-liver histogram and texture analysis on T1 maps improves the risk stratification of advanced fibrosis in NAFLD.

Eur Radiol 2021 Mar 8;31(3):1748-1759. Epub 2020 Sep 8.

Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197 Ruijin Er Road, Shanghai, 200025, China.

Objectives: To assess whole-liver texture analysis on T1 maps for risk stratification of advanced fibrosis in patients with suspected nonalcoholic fatty liver disease (NAFLD).

Methods: This retrospective study included 53 patients. Histogram and texture parameters (volume, mean, SD, median, 5th percentile, 95th percentile, skewness, kurtosis, diff-entropy, diff-variance, contrast, and entropy) of T1 maps were calculated based on the semi-automatically segmented whole-liver volume. A two-step approach combining the Nonalcoholic Fatty Liver Disease Fibrosis Score (NFS) and Fibrosis-4 Index (FIB-4) with the liver stiffness measurement (LSM) for the risk stratification was used. Univariate analysis was performed to identify significant parameters. Logistic regression models were then run on the significant features. Diagnostic performance was evaluated with receiver operating characteristic (ROC) analysis.

Results: In total, 33 (62%) subjects had a low risk and 20 (38%) subjects had an intermediate-to-high risk of advanced fibrosis. The following significantly different parameters with the best performance were diff-entropy, entropy, and diff-variance, with AUROC 0.837 (95% CI 0.73-0.95), 0.821 (95% CI 0.71-0.94), and 0.807 (95% CI 0.69-0.93). The optimal combination of median, 5th percentile, and diff-entropy as a multivariate model improved the diagnostic performance to diagnose an intermediate-to-high risk of advanced fibrosis with AUROC 0.902(95% CI 0.79-0.97).

Conclusions: Parameters obtained by histogram and texture analysis of T1 maps may be a noninvasive analytical approach for stratifying the risk of advanced fibrosis in NAFLD.

Key Points: • Variable flip angle (VFA) T1 mapping can be used to acquire 3D T1 maps within a clinically acceptable duration. • Whole-liver histogram and texture parameters on T1 maps in patients with NAFLD can distinguish those with an intermediate-to-high risk of advanced fibrosis. • The multivariate model of combination of texture parameters improved the diagnostic performance for a high risk of advanced fibrosis and clinical parameters offer no added value to the multivariate model.
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http://dx.doi.org/10.1007/s00330-020-07235-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880972PMC
March 2021

NiMOF-derived oxygen vacancy rich NiO with excellent capacitance and ORR/OER activities as a cathode material for Zn-based hybrid batteries.

Dalton Trans 2020 Sep;49(35):12441-12449

Department of Chemistry, College of Science, Northeastern University, Shenyang, 110819, P.R. China.

An Ni-Zn battery is a distinguished member in the family of closed Zn-based batteries due to its ideal power density and voltage. However, when it is employed as a power supply for electric vehicles, its defects in terms of specific capacitance and energy density become obvious. Herein, to resolve this problem, a hybrid battery system was created through a combination of Ni-Zn and Zn-air batteries at the cell level. In a hybrid battery system, oxygen vacancy rich NiO with S,N co-modified mesoporous carbon as a matrix was used as the cathode material. This cathode material showed a high specific capacitance of 202.1 mA h g-1 at 1.0 A g-1. When the current density reduces to 20 A g-1, this value decreases to 130.2 mA h g-1, which implies that 64.4% of specific capacitance was retained. It also exhibits excellent OER and ORR activities. For the hybrid battery system, when the discharge process was carried out at 1 mA cm-2, there were two voltage plateaus at 1.72 and 1.12 V, which originated from Ni-Zn and Zn-air, respectively. In this case, its specific capacitance and energy density reaches 800.3 mA h g-1 and 961 W h kg-1, respectively. The hybrid battery also possesses perfect stability during multi-cycle charge-discharge tests. The construction of this hybrid battery system develops a new road to prepare a power supply device with high performance.
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http://dx.doi.org/10.1039/d0dt01153cDOI Listing
September 2020

Rapid quantitative determination of fentanyl in human urine and serum using a gold-based immunochromatographic strip sensor.

J Mater Chem B 2020 09;8(37):8573-8584

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

Fentanyl is a typical opioid that is used in surgical anesthesia. However, when abused, fentanyl can lead to addiction and even death. To better control the use of fentanyl, it is necessary to develop rapid and sensitive detection methods. In this study, an ultrasensitive monoclonal antibody (mAb) was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold-based immunochromatographic strip (CG-ICS) for the analysis of fentanyl in urine and serum. Under optimum conditions, the anti-fentanyl mAb belonging to the subtype of IgG2b showed a half-maximal inhibitory concentration (IC50) of 0.11 ng mL-1 and a linear range of detection of 0.020-0.50 ng mL-1. Fenanyl-spiked original urine and serum diluted eight times were used for the analysis of fentanyl by ic-ELISA and CG-ICS. IC50 from the standard curves was 0.46 ng mL-1 for urine and 2.6 ng mL-1 for serum in ic-ELISA and 1.6 ng mL-1 for urine and 6.27 ng mL-1 for serum in CG-ICS. The recovery test revealed that the ic-ELISA and CG-ICS, with a recovery rate of 87.0-108.4% and a coefficient of variation of 3.3-10.9%, were the same reliable tools as the liquid chromatography tandem mass spectrometry for fentanyl analysis in real samples.
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http://dx.doi.org/10.1039/d0tb01509aDOI Listing
September 2020

Association between sickle cell disease and dental caries: a systematic review and meta-analysis.

Hematology 2020 Dec;25(1):309-319

Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, People's Republic of China.

Although dental caries has been widely reported in individuals with sickle cell disease (SCD), there is still controversial in the literature regarding the association between SCD and dental caries. The aim of this systematic review was to investigate whether individuals with SCD have more dental caries than individuals with non-SCD. PubMed and Embase databases were searched for eligible studies. The parameters of the permanent decayed, missing and filled teeth (DMFT) index and the permanent decayed, missing and filled surface (DMFS) index were considered as outcome measures. The overall meta-analyses of the DMFT and DMFS index and various subgroup analyses (caries components, age, and genotypes) of DMFT index were performed to calculate the weighted mean differences (WMD) between patients with SCD and non-SCD individuals. A total of 9 studies covering 1478 individuals were included in this meta-analysis. The results of overall meta-analyses indicated that the scores of the DMFT and DMFS index were not significantly different between patients with SCD and non-SCD participants. The results of subgroup analyses by caries components, age, and genotypes showed no significant difference in most items. The result of the missing teeth was significantly lower in patients with SCD than in non-SCD individuals (WMD, -0.14; 95% confidence interval [CI], -0.25 to -0.03;  = 0.01). The results revealed that compared with non-SCD individuals, patients with SCD did not suffer from worse dental caries. Considering the limitations, further well-designed studies are necessary to reveal the association between SCD and dental caries.
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http://dx.doi.org/10.1080/16078454.2020.1748927DOI Listing
December 2020

Possible involvement of crosstalk between endometrial cells and mast cells in the development of endometriosis via CCL8/CCR1.

Biomed Pharmacother 2020 Sep 8;129:110476. Epub 2020 Jul 8.

Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310006 PR China. Electronic address:

Background: The density and the activity of mast cells are associated with endometriosis. However, the role of mast cells on the pathogenesis of endometriosis remains unclear. Our study aims to investigate whether endometrial cells interact with mast cells and the involvement of their crosstalk in the development of endometriosis.

Methods: The transwell assay was applied to investigate the effect of mast cells on the migratory ability of human primary endometrial cells. Mast cells were cocultured with endometrial epithelial and stromal cells respectively and total RNAs were isolated and subjected to mRNA sequencing. Next, the transwell assay, CCK-8, and tube formation were applied to study the role of CCL8 on the endometrial and endothelial cells in vitro. The mouse model was also established to confirm the role of CCL8 in the development and angiogenesis of endometriosis.

Results: CCL8 was up-regulated in mast cells when cocultured with endometrial cells. CCL8 was highly expressed in the ectopic endometrium and the serum of patients with endometriosis. CCL8 promoted the migratory ability of endometrial epithelial and stromal cells and increased the proliferation, migration, and tube formation of endothelial cells. CCR1, the receptor of CCL8, was over-expressed in the ectopic endometrium and colocalized with blood vessels in ovarian endometriomas. The inhibition of CCR1 suppressed the development and angiogenesis of endometriosis in vivo.

Conclusion: The crosstalk between endometrial cells and mast cells in the development of endometriosis via CCL8/CCR1 was demonstrated, thereby providing a new treatment strategy for endometriosis.
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http://dx.doi.org/10.1016/j.biopha.2020.110476DOI Listing
September 2020

Photodegradation of polychlorinated diphenyl sulfides (PCDPSs) under simulated solar light irradiation: Kinetics, mechanism, and density functional theory calculations.

J Hazard Mater 2020 11 15;398:122876. Epub 2020 May 15.

Jiangsu Provincial Center for Disease Control and Prevention, NO.172 Jiangsu Road, Jiangsu Nanjing 210023, PR China. Electronic address:

The direct photolysis of 25 individual polychlorinated diphenyl sulfides (PCDPSs) substituted with 1-7 chlorine atoms was investigated using a 500-W Xe lamp. Photolysis of PCDPSs followed pseudo-first-order kinetics, with the higher chlorinated diphenyl sulfides generally degrading faster than the lower chlorinated congeners. A quantitative structure-activity relationship model to predict the photolysis rates of PCDPSs was developed using 16 fundamental quantum chemical descriptors. We found that the substitution pattern for chlorine atoms, the dipole moment, and E - E were major factors in the photolysis of PCPDSs. The reaction kinetics, products, and photodegradation pathways of 2,2',3',4,5-pentachlorodiphenyl sulfide (PeCDPS) suggest hydroxylation, direct photooxidation, the C-S bond cleavage reaction, and hydroxyl substitution were mainly involved in the photodegradation process, leading to the formation of 13 intermediates, detected by an electrospray time-of-flight mass spectrometer. The initial reaction sites of PCDPSs under photolysis were rationalized by density functional theory calculations. Anions (Cl, SO, NO, and HCO) and Co had no influence on the removal of PeCDPS, while Fe, Cu, and HA decreased the photolysis efficiency of PeCDPS. This report is the first to develop a logk quantitative structure-property relationships (QSPR) model of 25 PCDPSs and to describe mechanistic pathways for the photolysis of PeCDPS.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122876DOI Listing
November 2020

Enriched Environment and Social Isolation Affect Cognition Ability via Altering Excitatory and Inhibitory Synaptic Density in Mice Hippocampus.

Neurochem Res 2020 Oct 3;45(10):2417-2432. Epub 2020 Aug 3.

College of Life Sciences and Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin, 300071, People's Republic of China.

The purpose of the study was to examine whether the underlying mechanism of the alteration of cognitive ability and synaptic plasticity induced by the housing environment is associated with the balance of excitatory/inhibitory synaptic density. Enriched environment (EE) and social isolation (SI) are two different housing environment, and one is to give multiple sensory environments, the other is to give monotonous and lonely environment. Male 4-week-old C57 mice were divided into three groups: CON, EE and SI. They were housed in the different cage until 3 months of age. Morris water maze and novel object recognition were performed. Long term potentiation (LTP), depotentiation (DEP) and local field potentials were recorded in the hippocampal perforant pathway and dentate gyrus (DG) region. The data showed that EE enhanced the ability of spatial learning, reversal learning and memory as well as LTP/DEP in the hippocampal DG region. Meanwhile, SI reduced those abilities and the level of LTP/DEP. Moreover, there were higher couplings of both phase-amplitude and phase-phase in the EE group, and lower couplings of them in the SI group compared to that in the CON group. Western blot and immunofluorescence analysis showed that EE significantly enhanced the level of PSD-95, NR2B and DCX; however, SI reduced them but increased GABAR and decreased DCX levels. The data suggests that the cognitive functions, synaptic plasticity, neurogenesis and neuronal oscillatory patterns were significantly impacted by housing environment via possibly changing the balance of excitatory and inhibitory synaptic density.
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http://dx.doi.org/10.1007/s11064-020-03102-2DOI Listing
October 2020

Rapid and Sensitive Immunochromatographic Method-Based Monoclonal Antibody for the Quantitative Detection of Metalaxyl in Tobacco.

ACS Omega 2020 Jul 14;5(29):18168-18175. Epub 2020 Jul 14.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

In this study, we prepared a monoclonal antibody (mAb) against metalaxyl (Met) with a half-maximum inhibitory concentration (IC) of 0.54 ng/mL based on a new hapten, and a gold nanoparticle-based immunochromatographic assay (GICA) was developed for the rapid detection of Met residues in tobacco. Under optimal conditions, even with the naked eye, one can see the semiquantitative analysis results. The naked eye detection limit of Met in tobacco is 25 μg/kg, and the detection threshold is 100 μg/kg. In addition, the cross-reactivity test shows that the mAb has good specificity for Met, and the GICA results have a good correlation with the indirect competitive enzyme-linked immunosorbent assay and liquid chromatography with tandem mass spectrometry test results, which show that the method is feasible and reliable and are more convenient and quicker than the methods using instrumentation for detection. Therefore, GICA may provide a useful tool for the rapid screening and detection of Met residues in tobacco.
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http://dx.doi.org/10.1021/acsomega.0c01782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392375PMC
July 2020

KMnO-mediated reactions for hexachlorophene in aqueous solutions: Direct oxidation, self-coupling, and cross-coupling.

Chemosphere 2020 Nov 20;259:127422. Epub 2020 Jun 20.

State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Jiangsu, Nanjing, 210023, PR China.

Hexachlorophene (HCP) is used in a range of general cleaning and disinfecting products and has received increased attention due to its damaging effect to the central nervous system in animals and its toxicity in humans. The chemical oxidation of HCP by KMnO was performed to systematically evaluate the effects of oxidant dose, pH, temperature, typical anions, humic acid (HA), and various matrices on HCP removal. The second-order rate constant for HCP was determined to be 4.83 × 10 M s at pH 7.0 and 25 °C. The presence of HA can inhibit the removal of HCP by KMnO, while Cl, NO, SO, PO, and CO have negligible effects. Degradation products analysis of the reaction, as well as theoretical calculations of HCP molecule and its phenoxy radical species, indicated that KMnO oxidation for HCP included a C-C bridge bond cleavage, hydroxylation, direct oxidation and self-coupling, and cross-coupling reactions. This study revealed that KMnO oxidation is an effective technique for eliminating HCP in real water and wastewater.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127422DOI Listing
November 2020