Publications by authors named "Xinxin Li"

345 Publications

Sweroside Protects Against Myocardial Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress and Pyroptosis Partially via Modulation of the Keap1/Nrf2 Axis.

Front Cardiovasc Med 2021 19;8:650368. Epub 2021 Mar 19.

Department of Cardiovascular Ultrasound, The First Affiliated Hospital of China Medical University, Shenyang, China.

Sweroside, a secoiridoid glucoside extracted from Hara, is reported to possess antioxidant and anti-inflammatory activities. However, whether sweroside has a protective effect on myocardial ischemia-reperfusion (IR) injury is yet to be elucidated. The present study aimed to confirm the cardioprotective effect of sweroside and to identify its underlying mechanism. H9c2 cells were pretreated with sweroside and then underwent hypoxia-reoxygenation. Cell Counting Kit-8, creatine kinase-myocardial band (CK-MB) and lactate dehydrogenase (LDH) assays were conducted to detect cell viability and myocardial injury, respectively. The Langendorff method was used to induce myocardial IR injury . Triphenyltetrazolium chloride staining was performed to detect myocardial infarct size, while protein expression was analyzed using western blotting. Overall, the results indicated that sweroside pretreatment dose-dependently led to a significant enhancement in cell viability, a decrease in release of CK-MB and LDH, a reduction in infarct size, and an improvement in cardiac function. Additionally, sweroside pretreatment caused a marked suppression of oxidative stress, as evidenced by the fact that sweroside decreased the accumulation of reactive oxygen species and malondialdehyde, while enhancing the activities of superoxide dismutase and glutathione peroxidase. Moreover, sweroside was found to notably repress pyroptosis, as sweroside blocked pore formation in the cell membrane, inhibited caspase-1 and interleukin (IL)-1β activity, and decreased the expression levels of NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD, cleaved caspase-1, and IL-1β. Mechanistically, it was found that sweroside inhibited Kelch-like ECH-associated protein 1 (Keap1) and induced nuclear factor E2-associated factor 2 (Nrf2) nuclear translocation. Furthermore, the inhibition of oxidative stress and pyroptosis by sweroside could be abrogated via the inhibition of Nrf2 expression, which suggested that the protective effect induced by sweroside was Nrf2-dependent. The present study demonstrated that sweroside pretreatment could protect against myocardial IR injury by inhibiting of oxidative stress and NLRP3 inflammasome-mediated pyroptosis partially via modulation of the Keap1/Nrf2 axis.
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http://dx.doi.org/10.3389/fcvm.2021.650368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017130PMC
March 2021

Laser Grinding of Single-Crystal Silicon Wafer for Surface Finishing and Electrical Properties.

Micromachines (Basel) 2021 Mar 4;12(3). Epub 2021 Mar 4.

School of Mechanical Engineering and Automation, Beihang University, 37 Xueyuan Road, Beijing 100083, China.

In this paper, we first report the laser grinding method for a single-crystal silicon wafer machined by diamond sawing. 3D laser scanning confocal microscope (LSCM), X-ray diffraction (XRD), scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), laser micro-Raman spectroscopy were utilized to characterize the surface quality of laser-grinded Si. Results show that SiO layer derived from mechanical machining process has been efficiently removed after laser grinding. Surface roughness Ra has been reduced from original 400 nm to 75 nm. No obvious damages such as micro-cracks or micro-holes have been observed at the laser-grinded surface. In addition, laser grinding causes little effect on the resistivity of single-crystal silicon wafer. The insights obtained in this study provide a facile method for laser grinding silicon wafer to realize highly efficient grinding on demand.
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http://dx.doi.org/10.3390/mi12030262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000081PMC
March 2021

A review of current and emerging approaches for Quaternary marine sediment dating.

Sci Total Environ 2021 Mar 18;780:146522. Epub 2021 Mar 18.

Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

Dating methodologies for Quaternary marine sediments play increasingly important roles in the reconstruction of paleoenvironments and paleoclimate in (paleo)oceanography. Previous reviews or studies have focused mainly on one or two methodologies, and their applications in one specific environment. With the continuing technological and methodological advances in different methods over the past few decades, an up-to-date comparison of the pros and cons of each dating methodology is needed to clearly understand their applications in marine geoscience research. In this review, we first briefly summarized the common methods of absolute dating and relative dating. These are (1) radioisotope dating with different half-lives using natural nuclides of Th, Pb, Th, and Ra, cosmogenic nuclides of Be, C, Be, Si, Al, Cl and Ne, and the artificial radionuclides of Cs, Pu, Am and I that have been induced by atmospheric nuclear tests, accidents in nuclear plants, and discharges of radioactive wastes; (2) radiation exposure dating of luminescence and electron paramagnetic resonance (ESR) dating; and (3) stratigraphic dating of δO and paleomagnetic sequence. Applications and limitations from the marine terraces, estuaries, to hadal trenches have been summarized to each technique in the study of Quaternary marine geoscience extending from the Anthropocene through the Pleistocene. Finally, we introduced some emerging event dating methods, namely the arrivals of microplastics, mercury isotopes, and organic pollutant deposition that all appeared after the industrial resolution in our now changing ocean influenced by acidification, global warming, and anthropogenic activities. We ended by discussing future perspectives for reliable and high-resolution chronology by interdisciplinary methods including computer programming to better understand the natural geological evolution and predict the future changes in earth science.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146522DOI Listing
March 2021

Susceptibility of Isolates from the China-Myanmar Border Area to Piperaquine and Association with Candidate Markers.

Antimicrob Agents Chemother 2021 Mar 8. Epub 2021 Mar 8.

Department of Internal Medicine, Morsani College of Medicine, University of South Florida, 3720 Spectrum Boulevard, Suite 304, Tampa, FL, USA

from the Greater Mekong subregion has evolved resistance to the artemisinin-based combination therapy dihydroartemisinin and the partner drug piperaquine. To monitor the potential westward spread or independent evolution of piperaquine resistance, we evaluated the susceptibility of 120 isolates collected at the China-Myanmar border during 2007-2016. The parasite isolates displayed a relatively wide range of piperaquine susceptibility estimates. While 56.7% of the parasites showed bimodal drug response curves, all but five generated area-under-the-curve (AUC) estimates consistent with a susceptible phenotype. Using the piperaquine survival assay (PSA), 5.6% parasites showed reduced susceptibility. Of note, parasites from 2014-2016 showed the highest AUC value and the highest proportion with a bimodal curve, suggesting falling effectiveness in these later years. Unsupervised K-mean analysis of the combined data assigned parasites into three clusters and identified significant correlations between IC, IC and AUC values. No parasites carried the E415G mutation in a putative exo-nuclease, new mutations in PfCRT, or amplification of the genes, suggesting mechanisms of reduced piperaquine susceptibility that differ from those described in other countries of the region. The association of increased AUC, IC, and IC values with major PfK13 mutations (F446I and G533S) suggests that piperaquine resistance may evolve in these PfK13 genetic backgrounds. Additionally, the Pfmdr1 F1226Y mutation was associated with significantly higher PSA values. Further elucidation of piperaquine resistance mechanisms and continuous surveillance are warranted.
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http://dx.doi.org/10.1128/AAC.02305-20DOI Listing
March 2021

Functions of Forkhead Box O on Glucose Metabolism in Abalone and Its Responses to High Levels of Dietary Lipid.

Genes (Basel) 2021 Feb 20;12(2). Epub 2021 Feb 20.

The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, China.

The forkhead box O (FoxO) subfamily is a member of the forkhead transcription factor family. It has regulation functions in glucose metabolism in mammals and fish. In the present study, a gene of the homolog in abalone was cloned. A conservative forkhead (FH) domain and a transactivation (FoxO-TAD) domain were identified. Abalone -specific siRNA (small interfering RNA) was injected to investigate the functions of on glucose metabolism. Knockdown of inhibited expression of phosphoenolpyruvate carboxykinase () and significantly increased expressions of hexokinase () and pyruvate kinase (), but it failed to inhibit the relative mRNA level of glucose-6-phosphatase (). Then, a 100-day feeding trial was conducted to investigate the response of and glucose metabolism in abalone fed with 1.57% (LFD, low-fat diet), 3.82% (MFD, middle-fat diet) and 6.72% (HFD, high-fat diet) of dietary lipid, respectively. The insulin-signaling pathway (AKT) was depressed and FoxO was activated by the HFD, but it did not inhibit glycolysis () or improved gluconeogenesis significantly (). At the same time, impaired hepatopancreas glycogen storage raised hemolymph glucose levels. In conclusion, abalone can be regulated by dietary lipid and can regulate gluconeogenesis or glycolysis in response to changes of dietary lipid levels, in which glycogen metabolism plays an important role.
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http://dx.doi.org/10.3390/genes12020297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924355PMC
February 2021

Near-Infrared Light-Triggered Thermosensitive Liposomes Modified with Membrane Peptides for the Local Chemo/Photothermal Therapy of Melanoma.

Onco Targets Ther 2021 25;14:1317-1329. Epub 2021 Feb 25.

Department of Clinical Medicine, Xuzhou Medical University, Xuzhou, 221004, People's Republic of China.

Purpose: A near-infrared (NIR)-triggered trans-activating transcriptional activator (TAT)-based targeted drug delivery system for the combined chemo/photothermal therapy of melanoma, namely, TAT-TSL-TMZ (temozolomide)/IR820, was developed for the first time.

Methods: TAT-TSL-TMZ/IR820 liposomes were synthesized via thin-film dispersion and sonication. IR820 and TMZ were encased in the inner layer and lipid bilayer of the liposomes, respectively.

Results: Dynamic light scattering results showed that the liposomes had an average hydrodynamic size of 166.9 nm and a zeta potential of -2.55 mV. The encapsulation rates of TMZ and IR820 were 35.4% and 28.6%, respectively. The heating curve obtained under near-infrared (NIR) laser irradiation showed that TAT-TSL-TMZ/IR820 liposomes had good photothermal conversion efficiency. The in vitro drug release curve revealed that NIR laser irradiation could accelerate drug release from TAT-TSL-TMZ/IR820 liposomes. The results of inverted fluorescence microscopy and flow cytometry proved that the uptake of TAT-TSL-TMZ/IR820 liposomes by human melanoma cells (MV3 cells) was concentration-dependent and that the liposomes modified with membrane peptides were more likely to be ingested by cells than unmodified liposomes. Confocal laser scanning microscopy indicated that TAT-TSL-TMZ/IR820 liposomes entered MV3 cells via endocytosis and was stored in lysosomes. In addition, TAT-TSL-TMZ/IR820 liposomes exposed to NIR laser showed 89.73% reduction in cell viability.

Conclusion: This study investigated the photothermal conversion, cell uptake, colocation and chemo/photothermal effect of TAT-TSL-TMZ/IR820 liposomes.
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http://dx.doi.org/10.2147/OTT.S287272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920603PMC
February 2021

Pre-surgical peripheral blood inflammation markers predict surgical site infection following mesh repair of groin hernia.

Medicine (Baltimore) 2021 Mar;100(9):e25007

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Shantou University Medical College, No. 57 Changping Rd, Shantou, China.

Abstract: Surgical site infection (SSI) is a costly postoperative complication with a decrease in the quality of life. We aimed to probe the predictive role of peripheral blood inflammation markers for SSI following mesh repair of groin hernia (GH).This retrospective study assessed the data of 1177 patients undergoing elective mesh repair of GH (open/laparoscopy) in the absence of antibiotic prophylaxis. The relation between demographics, surgical factors, pre-surgical laboratory results and the occurrence of SSI were investigated by univariate and multivariate analyses. Receiver operating characteristic analysis was performed to determine the optimal threshold of parameters and compare their veracity.The overall SSI rate was 3.2% with 1-year follow-up (38 superficial and 1 deep SSI). Patients with SSI had significant higher pre-surgical neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) than those without (P = .029 and P = .045, respectively); their NLR and PLR correlated positively with postoperative total days of antibiotic treatment for SSI (r = .689, P = .000; r = .493, P = .001; respectively). NLR and PLR had larger areas under the receiver operating characteristics curves than neutrophil (.875 vs. .601; P = .000; .726 vs. .601; P = .017). The combination of PLR and neutrophil/NLR raised the predictive sensitivity of PLR for SSI (sensitivity: PLR: 74.36%; PLR + neutrophil: 82.05%; PLR + NLR: 83.57%). On multivariate analyses, higher preoperative NLR (cut-off 2.44) and PLR (cut-off 125.42) were independent predictors for SSI.Higher pre-surgical NLR and PLR may be valuable predictors for SSI following elective mesh repair of GH.
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http://dx.doi.org/10.1097/MD.0000000000025007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939215PMC
March 2021

Ten-Year Outcomes of Percutaneous Coronary Intervention Versus Coronary Artery Bypass Grafting for Patients with Type 2 Diabetes Mellitus Suffering from Left Main Coronary Disease: A Meta-Analysis.

Diabetes Ther 2021 Apr 27;12(4):1041-1054. Epub 2021 Feb 27.

Department of Cardiology, Peking University People's Hospital, Beijing, People's Republic of China.

Introduction: In this meta-analysis, we aimed to systematically compare the 10-year outcomes of percutaneous coronary intervention (PCI) versus coronary artery bypass grafting (CABG) in patients with type 2 diabetes mellitus (T2DM) suffering from left main coronary artery disease (LMCD).

Methods: Medical Literature Analysis and Retrieval System Online (MEDLINE), http://www.ClinicalTrials.gov , Excerpta Medica dataBASE (EMBASE), Cochrane Central, Web of Science, and Google scholar were searched for publications comparing 10-year outcomes of PCI versus CABG in patients with T2DM suffering from LMCD. Cardiovascular outcomes were considered as the clinical endpoints. Statistical analysis was carried out using RevMan software (version 5.4). Risk ratios (RR) with 95% confidence intervals (CI) were used to represent the data after analysis.

Results: Eight studies (three randomized trials and five observational studies) with a total number of 3835 participants with T2DM were included in this analysis; 2340 participants were assigned to the PCI group and 1495 participants were assigned to the CABG group. Results of this analysis showed that mortality (RR 0.85, 95% CI 0.73-1.00; P = 0.05), myocardial infarction (RR 0.53, 95% CI 0.35-0.80; P = 0.002), repeated revascularization (RR 0.34, 95% CI 0.26-0.46; P = 0.00001), and target vessel revascularization (RR 0.26, 95% CI 0.18-0.38; P = 0.00001) were significantly higher with PCI when compared to CABG in these patients with diabetes and LMCD. Major adverse cardiac and cerebrovascular events were also significantly higher with PCI at 10 years (RR 0.67, 95% CI 0.49-0.92; P = 0.01). However, CABG was associated with a significantly higher risk of stroke (RR 2.16, 95% CI 1.39-3.37; P = 0.0007).

Conclusions: During a long-term follow-up time period of 10 years, PCI was associated with worse clinical outcomes compared to CABG in these patients with T2DM suffering from LMCD. However, a significantly higher risk of stroke was observed with CABG. This piece of information might be vital in order to carefully choose and prevent complications following revascularization in such patients.
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http://dx.doi.org/10.1007/s13300-021-01025-xDOI Listing
April 2021

Long Non-Coding RNA JPX Contributes to Tumorigenesis by Regulating miR-5195-3p/VEGFA in Non-Small Cell Lung Cancer.

Cancer Manag Res 2021 12;13:1477-1489. Epub 2021 Feb 12.

Department of Oncology, The People's Hospital of Shouguang, Weifang, People's Republic of China.

Background: Lung cancer is the most frequently diagnosed cancer. Of all lung cancers, 80-85% are verified as non-small-cell lung cancer (NSCLC). Just proximal to X-inactive specific transcript (JPX), functions as lncRNA, contributed to tumor progression and suggested a poor prognosis in NSCLC. However, the pathogenesis of JPX involved in NSCLC is still unclear.

Methods: The expressions of JPX, miR-5195-3p, and Vascular endothelial growth factor A (VEGFA) were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Proliferation, colony number, apoptosis, invasion, and migration were analyzed by Cell Counting Kit-8 (CCK-8), colony formation, flow cytometry, transwell, and wound healing assays, severally. The protein levels of VEGFA, E-cadherin, N-cadherin, and Vimentin were detected by Western blot assay. The interaction between JPX, miR-5195-3p and VEGFA was predicted by starBase, and then verified by the dual-luciferase reporter, RNA Immunoprecipitation (RIP) and RNA pull-down assay. The biological role of JPX on NSCLC tumor growth was assessed by the xenograft tumor model in vivo.

Results: JPX and VEGFA were upregulated, and miR-5195-3p was downregulated in NSCLC. JPX induced proliferation, colony number, invasion, migration, epithelial-mesenchymal transition (EMT), and inhibited apoptosis of NSCLC cells. JPX is directly bound to miR-5195-3p. JPX regulated NSCLC cell proliferation, apoptosis and EMT by modulating miR-5195-3p. miR-5195-3p hindered NSCLC cells proliferation, EMT and accelerated apoptosis by directly targeting VEGFA. JPX silencing hindered the cell growth of NSCLC in vivo.

Conclusion: JPX facilitated proliferation, colony number, invasion, migration, EMT, and repressed apoptosis by miR-5195-3p/VEGFA axis, offering a possible therapeutic strategy for NSCLC.
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http://dx.doi.org/10.2147/CMAR.S255317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886111PMC
February 2021

Biosorption capacity of Mucor circinelloides bioaugmented with Solanum nigrum L. for the cleanup of lead, cadmium and arsenic.

Ecotoxicol Environ Saf 2021 Apr 3;212:112014. Epub 2021 Feb 3.

School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China. Electronic address:

The biosorption and bioaugmentation performances of Mucor circinelloides were investigated under different contact time, initial metal(loid) concentration and species. The microbe-plant interaction appeared synergistic with enhancing plant growth and alleviating oxidative damages induced by lead, cadmium and arsenic. The bioaugmentation with M. circinelloides led to significant immobilization on lead, cadmium and arsenic as indicated by the decreases of metal(loid) transfer and bioavailability in plant-microbe aqueous system. Lead, cadmium and arsenic were mainly allocated on cell wall and a few parts entered into intercellular system, suggesting cell wall adsorption and intracellular bioaccumulation served as the main mechanisms of M. circinelloides. The adsorption kinetics and isotherms on lead, cadmium and arsenic were fitted well with the pseudo-second-order and Langmuir models, with the maximum adsorption capacities of 500, 15.4 and 29.4 mg·g fungal biomass at pH 6.0 and 25 ℃. The optimum initial concentration and contact time were 300-10-20 mg·L and 2 h. This study provides a basis for M. circinelloides as a promising adsorbent and bioaugmented agent for the cleanup of soil/aqueous environment contaminated with lead, cadmium and arsenic.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112014DOI Listing
April 2021

The lncRNA MALAT1 participates in regulating coronary slow flow endothelial dysfunction through the miR-181b-5p-MEF2A-ET-1 axis.

Vascul Pharmacol 2021 Feb 2:106841. Epub 2021 Feb 2.

Department of Neurobiology, School of Life Sciences, China Medical University, Shenyang, China; Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, China; Key Laboratory of Medical Cell Biology, Ministry of Education of China, China Medical University, Shenyang, China.

Background: Coronary slow flow (CSF) refers to coronary arteries with no obvious stenosis but have slow coronary flow without effective treatment. The main cause of CSF is endothelial dysfunction. The long non-coding RNA (lncRNA) MALAT1 is involved in regulating endothelial dysfunction, but its role in CSF endothelial dysfunction is still unclear.

Methods: We included 41 CSF patients and 37 controls in the study, who all underwent coronary angiography, echocardiography, and brachial artery flow-mediated dilatation (FMD) examination. Human umbilical vein endothelial cells (HUVECs) stimulated by oxygen-glucose deprivation were used as CSF-induced HUVECs. Plasma endothelin-1 (ET-1) concentrations were determined by enzyme-linked immunosorbent assay (ELISA). The expression levels of MALAT1, miR-181b-5p, myocyte enhancer factor 2A (MEF2A), and ET-1 were measured by qRT-PCR or western blotting. Cell proliferation was determined by 5-ethynyl-2'-deoxyuridine (EdU) and Cell Counting Kit-8 (CCK-8) assays. Apoptosis was examined by flow cytometry. The relationship between miR-181b-5p and MALAT1 or MEF2A was verified by dual-luciferase reporter assay. MEF2A binding directly to the ET-1 promoter region was verified via chromatin immunoprecipitation (ChIP) assay.

Results: MALAT1 and ET-1 were increased, and miR-181b-5p was decreased in the peripheral blood of the CSF patients, and could be used as predictors of CSF. In the CSF-induced HUVECs, MALAT1 was highly expressed, and MALAT1 knockdown improved endothelial function. In contrast, miR-181b-5p was downregulated in the CSF-induced HUVECs, and miR-181b-5p overexpression improved endothelial function. While MEF2A was highly enriched in CSF-induced HUVECs, MEF2A knockdown reduced ET-1 and increased the endothelial function of CSF-induced HUVECs as a transcriptional regulator of ET-1. MALAT1 modulated MEF2A expression positively by sponging miR-181b-5p.

Conclusions: Endothelial function is reduced in CSF. MALAT1 participates in regulating CSF endothelial dysfunction through the miR-181b-5p-MEF2A-ET-1 axis, and could provide a new target for CSF treatment.
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http://dx.doi.org/10.1016/j.vph.2021.106841DOI Listing
February 2021

Dynamics of binding ability prediction between spike protein and human ACE2 reveals the adaptive strategy of SARS-CoV-2 in humans.

Sci Rep 2021 02 4;11(1):3187. Epub 2021 Feb 4.

The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a novel coronavirus causing the COVID-19 pandemic in 2020. High adaptive plasticity on the spike protein of SASR-CoV-2 enables it to transmit across different host species. In the present study, we collected 2092 high-quality genome sequences of SARS-CoV-2 from 160 regions in over 50 countries and reconstructed their phylogeny. We also analyzed the polymorphic interaction between spike protein and human ACE2 (hACE2). Phylogenetic analysis of SARS-CoV-2 suggests that SARS-CoV-2 is probably originated from a recombination event on the spike protein between a bat coronavirus and a pangolin coronavirus that endows it humans infectivity. Compared with other regions in the S gene of SARS-CoV-2, the direct-binding sites of the receptor-binding domain (RBD) is more conserved. We focused on 3,860 amino acid mutations in spike protein RBD (T333-C525) of SARS-CoV-2 and simulated their differential stability and binding affinity to hACE2 (S19-D615). The results indicate no preference for SARS-CoV-2 infectivity on people of different ethnic groups. The variants in the spike protein of SARS-CoV-2 may also be a good indicator demonstrating the transmission route of SARS-CoV-2 from its natural reservoir to human hosts.
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http://dx.doi.org/10.1038/s41598-021-82938-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862608PMC
February 2021

Quantitative Structure-Activity Relationship of Nanowire Adsorption to SO Revealed by TEM Technique.

Nano Lett 2021 Feb 3;21(4):1679-1687. Epub 2021 Feb 3.

State Key Lab of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.

A quantitative structure-activity relationship (QSAR) is revealed based on the real-time sulfurization processes of ZnO nanowires observed via gas-cell transmission electron microscopy ( TEM). According to the TEM observations, the ZnO nanowires with a diameter of 100 nm (ZnO-100 nm) gradually transform into a core-shell nanostructure under SO atmosphere, and the shell formation kinetics are quantitatively determined. However, only sparse nanoparticles can be observed on the surface of the ZnO-500 nm sample, which implies a weak solid-gas interaction between SO and ZnO-500 nm. The QSAR model is verified with heat of adsorption (-Δ°) and aberration-corrected TEM characterization. With the guidance of the QSAR model, the following adsorbing/sensing applications of ZnO nanomaterials are explored: (i) breakthrough experiment demonstrates the application potential of the ZnO-100 nm sample for SO capture/storage; (ii) the ZnO-500 nm sample features good reversibility (RSD = 1.5%, = 3) for SO sensing, and the detection limit reaches 70 ppb.
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http://dx.doi.org/10.1021/acs.nanolett.0c04481DOI Listing
February 2021

Enhanced Detection of Genitourinary Cancers Using Fragmentation and Copy Number Profiles Obtained from Urinary Cell-Free DNA.

Clin Chem 2021 Jan;67(2):394-403

Key Laboratory of Genomics and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing China.

Background: Recent studies have reported that examining the fragmentation profiles (FP) of plasma cell-free DNA (cfDNA) further improves the clinical sensitivity of tumor detection. We hypothesized that considering the differences of the FP of urinary cfDNA would increase the clinical sensitivity of genitourinary (GU) cancer detection.

Methods: 177 patients with GU cancer and 94 individuals without tumors were enrolled in the discovery cohort. An independent validation dataset comprising 30 patients without tumors and 66 patients with GU cancer was also collected. We constructed an ensemble classifier, GUIDER, to detect and localize GU cancers using fragmentation and copy number profiles obtained from shallow whole-genome sequencing of urinary cfDNA.

Results: Urinary cfDNA of patients with GU cancer had a higher proportion of long fragments (209-280 bp) and a lower proportion of short fragments (140-208 bp) compared to controls. The overall mean classification accuracy of the FP was 74.62%-85.39% for different algorithms, and integration of the FP and copy number alteration (CNA) features further enhanced the classification of samples from patients with GU cancer. The mean diagnostic accuracy was further improved by the ensemble classifier GUIDER, which integrated the FP and CNA profiles and resulted in a higher mean accuracy (87.52%) compared to the analysis performed without FP features (74.62%). GUIDER performed well in an independent validation dataset.

Conclusions: The lengthening and shortening of urinary cfDNA within specific size ranges were identified in patients with GU cancer. Integration of the FP should further enhance the ability to use urinary cfDNA as a molecular diagnostic tool.
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http://dx.doi.org/10.1093/clinchem/hvaa283DOI Listing
January 2021

Double-Deck Metal Solenoids 3D Integrated in Silicon Wafer for Kinetic Energy Harvester.

Micromachines (Basel) 2021 Jan 12;12(1). Epub 2021 Jan 12.

State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.

A silicon-chip based double-deck three-dimensional (3D) solenoidal electromagnetic (EM) kinetic energy harvester is developed to convert low-frequency (<100 Hz) vibrational energy into electricity with high efficiency. With wafer-level micro electro mechanical systems (MEMS) fabrication to form a metal casting mold and the following casting technique to rapidly (within minutes) fill molten ZnAl alloy into the pre-micromachined silicon mold, the 300-turn solenoid coils (150 turns for either inner solenoid or outer solenoid) are fabricated in silicon wafers for saw dicing into chips. A cylindrical permanent magnet is inserted into a pre-etched channel for sliding upon external vibration, which is surrounded by the solenoids. The size of the harvester chip is as small as 10.58 mm × 2.06 mm × 2.55 mm. The internal resistance of the solenoids is about 17.9 Ω. The maximum peak-to-peak voltage and average power output are measured as 120.4 mV and 43.7 μW. The EM energy harvester shows great improvement in power density, which is 786 μW/cm3 and the normalized power density is 98.3 μW/cm3/g. The EM energy harvester is verified by experiment to be able to generate electricity through various human body movements of walking, running and jumping. The wafer-level fabricated chip-style solenoidal EM harvesters are advantageous in uniform performance, small size and volume applications.
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http://dx.doi.org/10.3390/mi12010074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828048PMC
January 2021

Aqueous solubility of Pb at equilibrium with hydroxypyromorphite over a range of phosphate concentrations.

Environ Sci Process Impacts 2021 Feb;23(1):170-178

Section of Soil and Crop Sciences, Cornell University, Ithaca, NY 14850, USA.

Hydroxypyromorphite (HPM) is a low-solubility Pb phosphate mineral that has the potential to limit solubility and bioavailability of Pb in soils and water. Because of reported uncertainty regarding the solubility product of this important mineral, we re-evaluated the solubility of Pb and activity of the free Pb2+ ion in aqueous suspensions of microcrystalline HPM equilibrated up to 30 days over a wide range of added soluble phosphate. A small addition of phosphate (0.1 mM) reduced Pb solubility as measured by ICP-OES, but greater phosphate additions (up to 50 mM) had no further effect in lowering HPM solubility. However, free Pb2+ ion activity measured by ion-selective electrode progressively decreased from about 10-6.5 with no added phosphate to 10-9 as soluble phosphate was increased. The effect of soluble phosphate in lowering Pb2+ activity is attributed to inhibited dissolution of HPM as well as increased Pb2+-phosphate ion pair formation in solution at higher solution concentrations of phosphate. Measurement of the ion activity products (IAP) of the solutions at equilibrium with HPM gave highly variable IAP values that were sensitive to pH and were generally not consistent with the reported solubility product of this mineral. The high variability of the IAPs for solutions with variable pH and phosphate concentrations indicates that dissolution-precipitation reactions of HPM are not described by a constant solubility product at equilibrium, possibly because of the incongruent dissolution behavior of this mineral at near-neutral pH.
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http://dx.doi.org/10.1039/d0em00430hDOI Listing
February 2021

CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma.

Int J Nanomedicine 2020 22;15:10401-10416. Epub 2020 Dec 22.

Department of Dermatology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, People's Republic of China.

Objective: Nanotechnology-based photodynamic therapy (PDT) is a relatively new anti-tumor strategy. However, its efficacy is limited by the hypoxic state in the tumor microenvironment. In the present study, a poly(lactic-co-glycolic acid) (PLGA) nanoparticle that encapsulated both IR820 and catalase (CAT) was developed to enhance anti-tumor therapy.

Materials And Methods: HA-PLGA-CAT-IR820 nanoparticles (HCINPs) were fabricated via a double emulsion solvent evaporation method. Dynamic light scattering (DLS), transmission electron microscopy (TEM), laser scanning confocal microscopy, and an ultraviolet spectrophotometer were used to identify and characterize the nanoparticles. The stability of the nanoparticle was investigated by DLS via monitoring the sizes and polydispersity indexes (PDIs) in water, PBS, DMEM, and DMEM+10%FBS. Oxygen generation measurement was carried out via visualizing the oxygen bubbles with ultrasound imaging system and an optical microscope. Inverted fluorescence microscopy and flow cytometry were used to measure the uptake and targeting effect of the fluorescent-labeled nanoparticles. The live-dead method and tumor-bearing mouse models were applied to study the HCINP-induced enhanced PDT effect.

Results: The results showed that the HCINPs could selectively target melanoma cells with high expression of CD44, and generated oxygen by catalyzing HO, which increased the amount of singlet oxygen, ultimately inhibiting tumor growth significantly.

Conclusion: The present study presents a novel nanoplatform for melanoma treatment.
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http://dx.doi.org/10.2147/IJN.S283515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764953PMC
February 2021

CD8+ T Cell Co-Expressed Genes Correlate With Clinical Phenotype and Microenvironments of Urothelial Cancer.

Front Oncol 2020 19;10:553399. Epub 2020 Nov 19.

Department of Urology, China Medical University, The First Hospital of China Medical University, Shenyang, China.

Purpose: To identify immune-related co-expressed genes that promote CD8 T cell infiltration in bladder cancer, and to explore the interactions among relevant genes in the tumor microenvironment.

Method: We obtained bladder cancer gene matrix and clinical information data from TCGA, GSE32894 and GSE48075. The "estimate" package was used to calculate tumor purity and immune score. The CIBERSORT algorithm was used to assess CD8 T cell proportions. Weighted gene co-expression network analysis was used to identify the co-expression modules with CD8 T cell proportions and bladder tumor purity. Subsequently, we performed correlation analysis among angiogenesis factors, angiogenesis inhibitors, immune inflammatory responses, and CD8 T cell related genes in tumor microenvironment.

Results: A CD8 T cell related co-expression network was identified. Eight co-expressed genes (, , , , , , , ) were identified as CD8 T cell-related genes that promoted infiltration of CD8 T cells, and were enriched in the MHC class I tumor antigen presentation process. The proteins level encoded by these genes (, , , , , and ) were lower in the high clinical grade patients, which suggested the clinical phenotype correlation both in mRNA and protein levels. These factors negatively correlated with angiogenesis factors and positively correlated with angiogenesis inhibitors. PD-1 and PD-L1 positively correlated with these genes which suggested PD-1 expression level positively correlated with the biological process composed by these co-expression genes. In the high expression group of these genes, inflammation and immune response were more intense, and the tumor purity was lower, suggesting that these genes were immune protective factors that improved the prognosis in patients with bladder cancer.

Conclusion: These co-expressed genes promote high levels of infiltration of CD8 T cells in an immunoproteasome process involved in MHC class I molecules. The mechanism might provide new pathways for treatment of patients who are insensitive to PD-1 immunotherapy due to low degrees of CD8 T cell infiltration.
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http://dx.doi.org/10.3389/fonc.2020.553399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713665PMC
November 2020

Thermodynamic Phase-like Transition Effect of Molecular Self-assembly.

J Phys Chem Lett 2021 Jan 14;12(1):126-131. Epub 2020 Dec 14.

State Key Lab of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, China.

The technique of self-assembled monolayers (SAMs) is frequently applied for grafting functional groups or area-selective deposition of thin films on a material surface. The formation and quality of SAMs are fundamentally determined by thermodynamic data, which are difficult to measure with available experimental methods. This work quantitatively extracted thermodynamic parameters including Δ°, Δ°, and Δ° during the SAMs construction process with an ultrasensitive resonant microcantilever as molecule-surface interactions real-time recording tool. By correlating the thermodynamic parameters with self-assembling temperatures, a new thermodynamic phase-like transition effect of molecular self-assembly has been first revealed. The sharp transition of the thermodynamic parameters defines the critical condition for SAMs formation. The thermodynamic data further provide optimized reaction conditions for constructing high-quality SAMs. The explored quantitative thermodynamic analysis method not only plays as criterion for SAM growth but also helps to fundamentally elucidate physicochemical mechanism of spontaneous self-assembly.
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http://dx.doi.org/10.1021/acs.jpclett.0c03248DOI Listing
January 2021

Halophilic to mesophilic adaptation of ubiquitin-like proteins.

FEBS Lett 2021 Feb 19;595(4):521-531. Epub 2020 Dec 19.

School of Life Sciences, Anhui University, Hefei, China.

Elucidating how proteins adapt from halophilic to mesophilic environments will enable a better understanding of protein evolution and folding. In this study, by directed evolution and site-directed mutagenesis of the halophilic ubiquitin-like protein (ULP) Samp2, we find that substitution of the prebiotic amino acid Asp31 by Gly is uniquely effective in the mesophilic adaptation of ULP. Sequence analysis shows that substitution of Asp/Glu in halophilic ULPs by Gly in mesophilic ULPs has higher occurrence than other substitutions, supporting the unique role of the substitution in the mesophilic adaptation of ULP. Molecular dynamic simulations indicate that the mesophilic adaptation might result from the effect of the substitution on the conformational flexibility of ULP.
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http://dx.doi.org/10.1002/1873-3468.14023DOI Listing
February 2021

Midwifery students' perceptions and experiences of learning in clinical practice: a qualitative review protocol.

JBI Evid Synth 2020 Dec 3. Epub 2020 Dec 3.

PR China Southern Centre for Evidence-based Nursing and Midwifery Practice: A JBI Centre of Excellence, Guangzhou City, Guangdong Province, PR China.

Objective: This systematic review aims to investigate and synthesize qualitative evidence related to midwifery students' perceptions and experiences of learning in clinical practice.

Introduction: Midwifery students are required to develop strong competencies during pre-registration education for future practice. Clinical placements provide a good opportunity for students to build essential practice capacities. Understanding the perceptions and experiences of midwifery students in clinical practice helps develop effective midwifery clinical educational strategies. A qualitative systematic review is therefore proposed to improve midwifery clinical education.

Inclusion Criteria: This proposed review will consider qualitative studies that have explored the perceptions and experiences of learning in clinical practice of midwifery students in all degrees. The search will be limited to English-language published and unpublished studies to the present.

Methods: This review will follow the JBI approach for qualitative systematic reviews. A three-stage search will be conducted to include published and unpublished literature. Databases to be searched include PubMed, Science Direct, Web of Science, CINAHL, PsycINFO, American Nurses Association, Google Scholar, ProQuest Dissertation & Theses, and Index to Theses in Great Britain and Ireland. Identified studies will be screened for inclusion in the review by two independent reviewers. Any disagreements will be resolved through discussion. Data will be extracted using a standardized tool. Data synthesis will adhere to the meta-aggregative approach to categorize findings. The categories will be synthesized into a set of findings that can be used to inform midwifery education.
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http://dx.doi.org/10.11124/JBIES-20-00131DOI Listing
December 2020

Tetragonal Superlattice of Elongated Rhombic Dodecahedra for Sensitive SERS Determination of Pesticide Residues in Fruit.

ACS Appl Mater Interfaces 2020 Dec 4;12(50):56350-56360. Epub 2020 Dec 4.

Key Laboratory of New Energy and Rare Earth Resource Utilization of State Ethnic Affairs Commission, Key Laboratory of Photosensitive Materials & Devices of Liaoning Province, School of Physics and Materials Engineering, Dalian Minzu University, Dalian 116600, China.

The self-assembly of plasmonic nanoparticles into highly ordered superlattices could pave the way toward novel nanomaterials for surface-enhanced Raman scattering (SERS). Here, we propose the formation of large-area superlattices of elongated rhombic dodecahedra in a vertical orientation via a controlled droplet evaporation process. Expectedly, the constant humidity of the experimental condition could control the evaporation speed of droplets and this procedure promotes the balance between driven depletion attraction and electrostatic repulsion in the system, leading to the generation of well-organized three-dimensional (3D) superlattices. The unique geometry of elongated rhombic dodecahedra could establish the tetragonal superlattices, which breaks the conventional hexagonal symmetry of gold nanorods. Specifically, the influence of the type and concentration of surfactants, the concentration of nanoparticles, and the amount of droplets on the preparation results were systematically investigated to find the optimal assembly parameters. Remarkably, such close-packed tetragonal arrays of vertically aligned elongated rhombic dodecahedra exhibit more excellent SERS performance compared with the traditional hexagonal superstructure of gold nanorods. Benefiting from the high sensitivity and reproducibility of elongated rhombic dodecahedron superlattices, their applications in the determination of pesticide residues in apple and grape peels were successfully demonstrated. As a result, this study may advance the production of innovative plasmonic nanomaterials for a broad range of fields.
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http://dx.doi.org/10.1021/acsami.0c17471DOI Listing
December 2020

The quality of dissolved organic matter extracted at different times from pig compost and its copper binding capacity based on EEM-PARAFAC.

Ecotoxicol Environ Saf 2021 Jan 3;207:111545. Epub 2020 Nov 3.

College of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China. Electronic address:

Dissolved organic matter extracted from compost tea, can be regarded as alternatives to inorganic fertilizers as well to be used as a washing agent for heavy metal polluted soil. However, the composition and quality of compost tea produced under different extraction time are still unknown. The objective of the current study was set to explore the influence of different extraction time (i.e., 6, 12, and 24 h) on the composition, quality, and copper binding capacity of compost tea originated from pig manure compost. The results indicated that the extraction time obviously influenced the phenolic, aromatic carboxylic, and polycyclic aromatic groups of compost tea. In addition, the compost tea undergo the shorter extraction time (i.e., 6 and 12 h) contained more protein and humic-like compositions. Among the all treatments tested herein, the compost tea produced from 12 h extraction time obviously exhibited higher aromaticity, molecular weight, and humification degree than other two treatments. The highest stability constant value (log K) was observed for the shortest extraction time, i.e., 6.08. According to the quality measures and copper binding capability, shorter extraction times (i.e., 6 and 12 h) would be suggested for compost tea production from pig manure compost.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111545DOI Listing
January 2021

miR169c-NFYA-C-ENOD40 modulates nitrogen inhibitory effects in soybean nodulation.

New Phytol 2021 03 15;229(6):3377-3392. Epub 2020 Dec 15.

Root Biology Center, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Legume crops contribute a great portion of clean nitrogen (N) to agro-ecosystems through symbiotic N fixation in the nodule; however, the nodulation is always inhibited by high N availability which is known as the N inhibitory effect through largely unknown mechanisms. We functionally investigated miR169c-GmNFYA-C-GmENOD40 under multiple N conditions in soybean (Glycine max) (ENOD, Early Nodulin; NFYA, Nuclear Factor-Y Subunit A). We elucidated their regulatory roles in soybean nodulation through analyzing expression patterns, micro-messenger RNA (miRNA-mRNA) interactions, phenotypes of transgenic soybean plants and genetic interactions. We found that miR169c expression was induced by high N, whereas its target GmNFYA-C was preferentially expressed in nodules and induced by rhizobium inoculation. Overexpression of miR169c inhibited nodulation through targeting 3'-UTR of GmNFYA-C, whereas knockout miR169c through CRISPR-cas9 promoted nodulation. However, overexpression of GmNFYA-C promoted soybean nodulation through facilitating rhizobium infection and increasing the expression of symbiotic signaling gene GmENOD40. Besides, GmNFYA-C directly induced the expression of GmENOD40. In addition, overexpression of GmNFYA-C without the target site of miR169c partially attenuated the inhibitory effect of high N on soybean nodulation. We discovered a new regulatory pathway involving the miR169c-NFYA-C-ENOD40 module that regulates soybean nodulation in response to N availability. This pathway provides substantial new insights into the mechanisms underlying the N inhibitory effect on nodulation.
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http://dx.doi.org/10.1111/nph.17115DOI Listing
March 2021

Bicycling Phase Recognition for Lower Limb Amputees Using Support Vector Machine Optimized by Particle Swarm Optimization.

Sensors (Basel) 2020 Nov 15;20(22). Epub 2020 Nov 15.

School of Engineering, Merz Court, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.

A novel method for recognizing the phases in bicycling of lower limb amputees using support vector machine (SVM) optimized by particle swarm optimization (PSO) is proposed in this paper. The method is essential for enhanced prosthetic knee joint control for lower limb amputees in carrying out bicycling activity. Some wireless wearable accelerometers and a knee joint angle sensor are installed in the prosthesis to obtain data on the knee joint and ankle joint horizontal, vertical acceleration signal and knee joint angle. In order to overcome the problem of high noise content in the collected data, a soft-hard threshold filter was used to remove the noise caused by the vibration. The filtered information is then used to extract the multi-dimensional feature vector for the training of SVM for performing bicycling phase recognition. The SVM is optimized by PSO to enhance its classification accuracy. The recognition accuracy of the PSO-SVM classification model on testing data is 93%, which is much higher than those of BP, SVM and PSO-BP classification models.
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http://dx.doi.org/10.3390/s20226533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696493PMC
November 2020

Specific inhibition of SHP2 suppressed abdominal aortic aneurysm formation in mice by augmenting the immunosuppressive function of MDSCs.

Life Sci 2021 Jan 12;265:118751. Epub 2020 Nov 12.

Clinical Laboratory, Hebei General Hospital, China. Electronic address:

Aims: To address the roles of SHP2 in regulating angiotensin II (Ang II) induced abdominal aortic aneurysm (AAA) and the potential molecular mechanisms.

Main Methods: AAA model was established in apolipoprotein E-deficient (apoE) mice infused with Ang II. Suprarenal aortic luminal diameters were ultrasonically measured to determine the presentation of AAA in mice. The inflammatory and immunosuppressive factors in serum were detected by ELISA. AAA lesion size, positive macrophages and elastic laminae degradation were examined by histological analysis. Myeloid-derived suppressor cells (MDSCs) were measured by flow cytometry after magnetic bead sorting. Bioinformatics analysis was applied to screen the crucial genes related the progression of AAA.

Key Findings: Treatment with PHPS1 (SHP2 inhibitor) significantly decreased the vascular diameter of AAA. Histological analysis showed that PHPS1 obviously reduced the Masson positive area, macrophages positive area, as well as the damage rate of elastic laminae. Moreover, PHPS1 suppressed the expression of INF-γ, TNF-α and MMPs, as well as elevated IL-10 and arginase-1 expression. Additionally, PHPS1 enhanced the expression of granulocytic MDSCs (G-MDSCs). By consulting with bioinformatics, STAT3 was selected. In G-MDSCs, PHPS1 stimulation obviously increased the phosphorylation level of STAT3, as well as elevated the protein expression of C/EBPβ and arginase-1. However, the above phenomena can be blocked after Stattic (STAT3 inhibitor) treatment.

Significance: SHP2 may affect the AAA progression by interfering with expansion and function of MDSCs to regulate the body immunity, which might afford a novel direction for the treatment of patients with AAA.
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http://dx.doi.org/10.1016/j.lfs.2020.118751DOI Listing
January 2021

Bioinformatic Analysis Identifies Potential Key Genes in the Pathogenesis of Melanoma.

Front Oncol 2020 16;10:581985. Epub 2020 Oct 16.

Kaifeng Key Laboratory for Infectious Diseases and Biosafety, Henan International Joint Laboratory of Nuclear Protein Regulation, Henan School of Basic Medical Sciences, Henan University College of Medicine, Kaifeng, China.

Melanoma is the deadliest skin tumor and is prone to distant metastases. The incidence of melanoma has increased rapidly in the past few decades, and current trends indicate that this growth is continuing. This study was aimed to explore the molecular mechanisms of melanoma pathogenesis and discover underlying pathways and genes associated with melanoma. We used high-throughput expression data to study differential expression profiles of related genes in melanoma. The differentially expressed genes (DEGs) of melanoma in GSE15605, GSE46517, GSE7553, and the Cancer Genome Atlas (TCGA) datasets were analyzed. Differentially expressed genes (DEGs) were identified by paired t-test. Then the DEGs were performed cluster and principal component analyses and protein-protein interaction (PPI) network construction. After that, we analyzed the differential genes through bioinformatics and got hub genes. Finally, the expression of hub genes was confirmed in the TCGA databases and collected patient tissue samples. Total 144 up-regulated DEGs and 16 down-regulated DEGs were identified. A total of 17 gene ontology analysis (GO) terms and 11 pathways were closely related to melanoma. Pathway of pathways in cancer was enriched in 8 DEGs, such as junction plakoglobin (JUP) and epidermal growth factor receptor (EGFR). In the PPI networks, 9 hub genes were obtained, such as loricrin (LOR), filaggrin (FLG), keratin 5 (KRT5), corneodesmosin (CDSN), desmoglein 1 (DSG1), desmoglein 3 (DSG3), keratin 1 (KRT1), involucrin (IVL), and EGFR. The pathway of pathways in cancer and its enriched DEGs may play important roles in the process of melanoma. The hub genes of DEGs may become promising melanoma candidate genes. Five key genes FLG, DSG1, DSG3, IVL, and EGFR were identified in the TCGA database and melanoma tissues. The results suggested that FLG, DSG1, DSG3, IVL, and EGFR might play important roles and potentially be valuable in the prognosis and treatment of melanoma. These hub genes might well have clinical significance as diagnostic markers.
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http://dx.doi.org/10.3389/fonc.2020.581985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596746PMC
October 2020

Soy isoflavone genistein inhibits hsa_circ_0031250/miR-873-5p/FOXM1 axis to suppress non-small-cell lung cancer progression.

IUBMB Life 2021 Jan 7;73(1):92-107. Epub 2020 Nov 7.

Department of Scientific Research, The First Affiliated Hospital of Henan University, Kaifeng, China.

The foods of plants provide the rich nutrition and have protective function in human diseases, including cancers. Genistein is a major isoflavone constituent in soybeans, which has an anti-cancer role in non-small-cell lung cancer (NSCLC). Nevertheless, the mechanism underlying the anti-cancer function of genistein in NSCLC remains largely unknown. NSCLC cells (H292 and A549) were exposed to genistein. Circular RNA hsa_circ_0031250 (circ_0031250), microRNA (miR)-873-5p and forkhead box M1 (FOXM1) abundances were examined via quantitative reverse transcription polymerase chain reaction and Western blotting. The function of genistein, circ_0031250, miR-873-5p, and FOXM1 on NSCLC progression was investigated via Cell Counting Kit-8, colony formation, transwell well, wound healing, flow cytometry, Western blotting and xenograft model. The target relationship was analyzed by dual-luciferase reporter analysis and RNA immunoprecipitation. Results showed that genistein inhibited NSCLC cell viability in dose-time-dependent patterns. circ_0031250 abundance was elevated in NSCLC samples and cell lines, and it was reduced via genistein exposure. circ_0031250 knockdown aggravated genistein-caused suppression of cell proliferation, migration and invasion and elevation of apoptosis. miR-873-5p expression was decreased in NSCLC samples and cells. miR-873-5p was targeted via circ_0031250, and miR-873-5p knockdown attenuated the influence of circ_0031250 silence on NSCLC progression in the presence of genistein. FOXM1 was regulated via circ_0031250/miR-873-5p axis. miR-873-5p constrained cell proliferation, migration and invasion and increased apoptosis via regulating FOXM1 in genistein-treated cells. circ_0031250 knockdown enhanced the inhibitive function of genistein on NSCLC cell growth in xenograft model. Collectively, genistein repressed NSCLC progression by modulating circ_0031250/miR-873-5p/FOXM1 axis.
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http://dx.doi.org/10.1002/iub.2404DOI Listing
January 2021

Diverse Gene Cassette Arrays Prevail in Commensal From Intensive Farming Swine in Four Provinces of China.

Front Microbiol 2020 14;11:565349. Epub 2020 Oct 14.

College of Veterinary Medicine, Northwest A&F University, Yangling, China.

Multiple-drug resistance bacteria containing antimicrobial resistance genes (ARGs) are a concern for public health. Integrons are bacterial genetic elements that can capture, rearrange, and express mobile gene cassettes responsible for the spread of ARGs. Few studies link genotype and phenotype of swine-related ARGs in the context of mobile gene cassette arrays among commensal () in nonclinical livestock isolates from intensive farms. In the present study, a total of 264 isolates were obtained from 330 rectal swabs to determine the prevalence and characteristics of antibiotic-resistant gene being carried by commensal in the healthy swine from four intensive farms at Anhui, Hebei, Shanxi, and Shaanxi, in China. Antimicrobial resistance phenotypes of the recovered isolates were determined for 19 antimicrobials. The isolates were commonly nonsusceptible to doxycycline (75.8%), tetracycline (73.5%), sulfamethoxazole-trimethoprim (71.6%), amoxicillin (68.2%), sulfasalazine (67.1%), ampicillin (58.0%), florfenicol (56.1%), and streptomycin (53.0%), but all isolates were susceptible to imipenem (100%). Isolates [184 (69.7%)] exhibited multiple drug resistance with 11 patterns. Moreover, 197 isolates (74.6%) were detected carrying the integron-integrase gene (1) of class 1 integrons. A higher incidence of antimicrobial resistance was observed in the 1-positive isolates than in the 1-negative isolates. Furthermore, there were 17 kinds of gene cassette arrays in the 70 integrons as detected by sequencing amplicons of variable regions, with 66 isolates (94.3%) expressing their gene cassettes encoding for multiple drug resistance phenotypes for streptomycin, neomycin, gentamicin, kanamycin, amikacin, sulfamethoxazole-trimethoprim, sulfasalazine, and florfenicol. Notably, due to harboring multiple, hybrid, and recombination cassettes, complex cassette arrays were attributed to multiple drug resistance patterns than simple arrays. In conclusion, we demonstrated that the prevalence of multiple drug resistance and the incidence of class 1 integrons were 69.7 and 74.6% in commensal isolated from healthy swine, which were lower in frequency than that previously reported in China.
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http://dx.doi.org/10.3389/fmicb.2020.565349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591504PMC
October 2020

Three-gene risk model in papillary renal cell carcinoma: a robust likelihood-based survival analysis.

Aging (Albany NY) 2020 11 5;12(21):21854-21873. Epub 2020 Nov 5.

Department of Urology, The First Hospital of China Medical University, Shenyang 110001, Liaoning, China.

Background: Papillary renal cell carcinoma (PRCC) accounts for 15% of all renal cell carcinomas. The molecular mechanisms of renal papillary cell carcinoma remain unclear, and treatments for advanced disease are limited.

Result: We built the computing model as follows: Risk score = 1.806 * - 0.355 * - 0.805 * . The 3-year AUC of overall survival was 0.917 in the training set (147 PRCC samples) and 0.760 in the test set (142 PRCC samples). Based on the robust model, M2 macrophages showed positive correlation with risk score, while M1 macrophages were the opposite. PRCC patients with low risk score showed higher tumor mutation burden. is a risk factor, and co-expression factors were enriched in cell proliferation and cancer-related pathways. Finally, the proliferation and invasion of PRCC cell line were decreased in the reduced group, and the differential expression was identified. is a potential risk biomarker which involved in cell proliferation in PRCC.

Conclusion: We conducted a study to develop a three gene model for predicting prognosis in patients with papillary renal cell carcinoma. Our findings may provide candidate biomarkers for prognosis that have important implications for understanding the therapeutic targets of papillary renal cell carcinoma.

Method: Gene expression matrix and clinical data were obtained from TCGA (The Cancer Genome Atlas), GSE26574, GSE2048, and GSE7023. Prognostic factors were identified using "survival" and "rbsurv" packages, and a risk score was constructed using Multivariate Cox regression analysis. The co-expression networks of the factors in model were constructed using the "WGCNA" package. The co-expression genes of factors were enriched and displayed the biological process. Based on this robust risk model, immune cells infiltration proportions and tumor mutation burdens were compared between risk groups. Subsequently, using the PRCC cell line, the role of TPX2 was determined by Cell proliferation assay, 5-Ethynyl-20-deoxyuridine assay and Transwell assay.
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http://dx.doi.org/10.18632/aging.104001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695399PMC
November 2020