Publications by authors named "Xinran Wang"

179 Publications

Protocol for a randomised controlled trial on the feasibility and effects of 10-hour time-restricted eating on cardiometabolic disease risk among career firefighters doing 24-hour shift work: the Healthy Heroes Study.

BMJ Open 2021 06 16;11(6):e045537. Epub 2021 Jun 16.

Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA

Introduction: Career firefighters experience chronic circadian rhythm disruption, increasing their risk of cardiometabolic disease. The recent discovery that eating patterns regulate circadian rhythmicity in metabolic organs has raised the hypothesis that maintaining a consistent daily cycle of eating and fasting can support circadian rhythms and reduce disease risks. Preclinical animal studies and preliminary clinical trials have shown promising effects of time-restricted eating (TRE) to reduce disease risk without compromising physical performance. However, there is a lack of research on TRE in shift workers including firefighters. This study aims to investigate the feasibility and efficacy of 10-hour TRE on health parameters that contribute to cardiometabolic disease risks among career firefighters who work on a 24-hour shift schedule.

Methods And Analyses: The Healthy Heroes Study is a randomised controlled parallel open-label clinical trial with 150 firefighters over 1 year. Firefighters are randomised with a 1:1 ratio to either the control or intervention group. The control group receives Mediterranean diet nutritional counselling (standard of care, 'SOC'). The intervention group receives the same SOC and a self-selected 10-hour TRE window. After the 2-week baseline, participants enter a 3-month monitored intervention, followed by a 9-month self-guided period with follow-up assessments. The impact of TRE on blood glucose, body weight, body composition, biomarkers (neuroendocrine, inflammatory and metabolic), sleep and mood is evaluated. These assessments occur at baseline, at the end of intervention and at 6, 9 and 12-month follow-ups. Temporal calorie intake is monitored with the smartphone application myCircadianClock throughout the study. Continuous glucose monitors, wrist-worn actigraphy device and questionnaires are used to monitor glucose levels, activity, sleep and light exposure.

Ethics And Dissemination: The study was approved by the Institutional Review Boards of the University of California San Diego and the Salk Institute for Biological Studies. Results will be disseminated through peer-reviewed manuscripts, reports and presentations.

Trial Registration Number: NCT03533023; Pre result.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/bmjopen-2020-045537DOI Listing
June 2021

Crystal Phase-Controlled Modulation of Binary Transition Metal Oxides for Highly Reversible Li-O Batteries.

Nano Lett 2021 Jun 1;21(12):5225-5232. Epub 2021 Jun 1.

Beijing Key Laboratory of Environmental Science and Engineering, School of Materials Science & Engineering, Beijing Institute of Technology, Beijing 100081, China.

Reducing charge-discharge overpotential of transition metal oxide catalysts can eventually enhance the cell efficiency and cycle life of Li-O batteries. Here, we propose that crystal phase engineering of transition metal oxides could be an effective way to achieve the above purpose. We establish controllable crystal phase modulation of the binary MnCoO by adopting a cation regulation strategy. Systematic studies reveal an unprecedented relevancy between charge overpotential and crystal phase of MnCoO catalysts, whereas a dramatically reduced charge overpotential (0.48 V) via a rational optimization of Mn/Co molar ratio = 8/2 is achieved. Further computational studies indicate that the different morphologies of LiO should be related to different electronic conductivity and binding of LiO on crystal facets of MnCoO catalysts, finally leading to different charge overpotential. We anticipate that this specific crystal phase engineering would offer good technical support for developing high-performance transition metal oxide catalysts for advanced Li-O batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.1c01276DOI Listing
June 2021

Mental Health Help-Seeking and Associated Factors Among Public Health Workers During the COVID-19 Outbreak in China.

Front Public Health 2021 11;9:622677. Epub 2021 May 11.

School of Public Health, Sun Yat-sen University, Guangzhou, China.

The COVID-19 outbreak in China has created multiple stressors that threaten individuals' mental health, especially among public health workers (PHW) who are devoted to COVID-19 control and prevention work. This study aimed to investigate the prevalence of mental help-seeking and associated factors among PHW using Andersen's Behavioral Model of Health Services Use (BMHSU). A cross-sectional survey was conducted among 9,475 PHW in five provinces across China between February 18 and March 1, 2020. The subsample data of those who reported probable mental health problems were analyzed for this report ( = 3,417). Logistic and hierarchical regression analyses were conducted to examine the associations of predisposing, enabling, need, and COVID-19 contextual factors with mental health help-seeking. Only 12.7% of PHW reported professional mental help-seeking during the COVID-19 outbreak. PHW who were older, had more days of overnight work, received psychological training, perceived a higher level of support from the society, had depression and anxiety were more likely to report mental help-seeking (OR range: 1.02-1.73, all < 0.05) while those worked in Centers for Disease Control and Prevention were less likely to seek help (OR = 0.57, < 0.01). The belief that mental health issues were not the priority (64.4%), lack of time (56.4%), and shortage of psychologists (32.7%) were the most frequently endorsed reasons for not seeking help. The application of BMHSU confirmed associations between some factors and PHW's mental health help-seeking. Effective interventions are warranted to promote mental health help-seeking of PHW to ameliorate the negative impact of mental illness and facilitate personal recovery and routine work.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2021.622677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144452PMC
June 2021

How can artificial intelligence models assist PD-L1 expression scoring in breast cancer: results of multi-institutional ring studies.

NPJ Breast Cancer 2021 May 26;7(1):61. Epub 2021 May 26.

Department of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Programmed death ligand-1 (PD-L1) expression is a key biomarker to screen patients for PD-1/PD-L1-targeted immunotherapy. However, a subjective assessment guide on PD-L1 expression of tumor-infiltrating immune cells (IC) scoring is currently adopted in clinical practice with low concordance. Therefore, a repeatable and quantifiable PD-L1 IC scoring method of breast cancer is desirable. In this study, we propose a deep learning-based artificial intelligence-assisted (AI-assisted) model for PD-L1 IC scoring. Three rounds of ring studies (RSs) involving 31 pathologists from 10 hospitals were carried out, using the current guideline in the first two rounds (RS1, RS2) and our AI scoring model in the last round (RS3). A total of 109 PD-L1 (Ventana SP142) immunohistochemistry (IHC) stained images were assessed and the role of the AI-assisted model was evaluated. With the assistance of AI, the scoring concordance across pathologists was boosted to excellent in RS3 (0.950, 95% confidence interval (CI): 0.936-0.962) from moderate in RS1 (0.674, 95% CI: 0.614-0.735) and RS2 (0.736, 95% CI: 0.683-0.789). The 2- and 4-category scoring accuracy were improved by 4.2% (0.959, 95% CI: 0.953-0.964) and 13% (0.815, 95% CI: 0.803-0.827) (p < 0.001). The AI results were generally accepted by pathologists with 61% "fully accepted" and 91% "almost accepted". The proposed AI-assisted method can help pathologists at all levels to improve the PD-L1 assay (SP-142) IC assessment in breast cancer in terms of both accuracy and concordance. The AI tool provides a scheme to standardize the PD-L1 IC scoring in clinical practice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41523-021-00268-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155065PMC
May 2021

Design, synthesis, and biological evaluation of 5-aminotetrahydroquinoline-based LSD1 inhibitors acting on Asp375.

Arch Pharm (Weinheim) 2021 May 13:e2100102. Epub 2021 May 13.

Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang, China.

The abnormal expression of lysine-specific histone demethylase 1 (LSD1) is associated with different cancer types, and LSD1 inhibitory activity seems to have high therapeutic potential in cancer treatment. Here, we report the design, synthesis, and biochemical evaluation of novel 5-aminotetrahydroquinoline-based LSD1 inhibitors. Among them, compounds A6, A8, B1-B5, and C4 showed preferable inhibitory effects on LSD1, with IC  = 0.19-0.82 µM. Several potent compounds were selected to evaluate their antiproliferative activity on A549 cells and MCF-7 cells with a high expression of LSD1. The potential binding modes of the compounds were revealed through molecular docking to rationalize the potency of compounds toward LSD1. Our data recognized that the 5-aminotetrahydroquinoline scaffold may serve as a starting point for developing potent LSD1 inhibitors for cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ardp.202100102DOI Listing
May 2021

Ultraeven Mo-Doped CoP Nanocrystals as Bifunctional Electrocatalyst for Efficient Overall Water Splitting.

Langmuir 2021 May 7;37(19):5986-5992. Epub 2021 May 7.

Laboratory for Low Dimensional Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.

To develop precious-metal-free bifunctional catalysts for overall water splitting, ultraeven Mo-doped CoP composites (Mo-CoP) have been fabricated by an in situ phosphorization protocol using CoMo-layered double hydroxide (CoMo-LDH) as the precursor. The ordered arrangement of cations in the CoMo-LDH could be easily phosphored and generate the ultraevenly dispersed Mo element within the CoP structure, resulting in the excellent bifunctional catalyst for overall water splitting. The hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) catalytic activities of the composites present an increase first and then a decreasing tendency with increased Mo doping content. Among all the Mo-doped CoP materials, the composite with a Mo/Co mole ratio of 1/2.3 presents the highest HER activity and stability in acidic conditions. At the current density of -10 mA·cm in 0.5 M HSO, the overpotential is only 116 mV. In addition, the composite also presents excellent HER and OER performance under alkaline conditions. The overpotential is 118 mV for HER and 317 mV for OER at 10 mA cm in 1 M KOH. It requires a cell voltage of 1.7 V to achieve a current density of 10 mA·cm and maintains a stable water-splitting current for at least 24 h, which is superior to most reported alkaline media. This simple and efficient synthetic approach could also be used for ultraeven doping between other transition metal ions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.1c00524DOI Listing
May 2021

Design, synthesis and biological evaluation of novel benzofuran derivatives as potent LSD1 inhibitors.

Eur J Med Chem 2021 Aug 24;220:113501. Epub 2021 Apr 24.

Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, 110016, Liaoning, China.

Lysine-specific demethylase 1 (LSD1) is a FAD-dependent enzyme, which has been proposed as a promising target for therapeutic cancer. Herein, a series of benzofuran derivatives were designed, synthesized and biochemical evaluated as novel LSD1 inhibitors based on scaffold hopping and conformational restriction strategy. Most of the compounds potently suppressed the enzymatic activities of LSD1 and potently inhibited tumor cells proliferation. In particular, the representative compound 17i exhibited excellent LSD1 inhibition at the molecular levels with IC = 0.065 μM, as well as anti-proliferation against MCF-7, MGC-803, H460, A549 and THP-1 tumor cells with IC values of 2.90 ± 0.32, 5.85 ± 0.35, 2.06 ± 0.27, 5.74 ± 1.03 and 6.15 ± 0.49 μM, respectively. The binding modes of these compounds were rationalized by molecular docking. Meanwhile, a preliminary druggability evaluation showed that compound 17i displayed favorable liver microsomal stability and weak inhibitory activity against CYPs at 10 μM. Remarkably, H460 xenograft tumors studies revealed that 17i demonstrated robust in vivo antitumor efficacy without significant side effects. All the results demonstrated that compound 17i could represent a promising lead for further development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejmech.2021.113501DOI Listing
August 2021

Improving Ki67 Assessment Concordance with AI-Empowered Microscope: A Multi-institutional Ring Study.

Histopathology 2021 Apr 11. Epub 2021 Apr 11.

Department of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Purpose: The nuclear proliferation biomarker Ki67 plays potential prognostic and predictive roles in breast cancer treatment. However, lack of inter-pathologist consistency in Ki67 assessment limits the clinical use of Ki67. This paper reports a solution utilizing an artificial intelligence (AI)-empowered microscope to improve Ki67 scoring concordance.

Methods: We developed an AI empowered microscope where the conventional microscope was equipped with AI algorithms and AI results were provided to pathologists in real time through augmented reality. We recruited 30 pathologists with various experience levels from 5 institutes to assess Ki67 label index on 100 Ki67 stained slides for invasive breast cancer patients. In the first round, pathologists conducted visual assessment on a conventional microscope; in the second round, they were assisted with reference cards; and in the third round, they were assisted with an AI-empowered microscope.

Results: Experienced pathologists had better reproducibility and accuracy (ICC = 0.864, mean error = 8.25%) than inexperienced pathologists (ICC = 0.807, mean error = 11.0%) in visual assessment. Moreover, with reference cards, inexperienced (ICC = 0.836, mean error = 10.7%) and experienced pathologists (ICC = 0.875, mean error = 7.56%) improved their reproducibility and accuracy. Finally, both experienced (ICC = 0.937, mean error = 4.36%) and inexperienced pathologists (ICC = 0.923, mean error = 4.71%) improved the reproducibility and accuracy significantly with the AI-empowered microscope.

Conclusion: AI-empowered microscope allows seamless integration of the AI solution into the clinical workflow and helps pathologists to obtain a higher consistency and accuracy for the Ki67 assessment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/his.14383DOI Listing
April 2021

1D Mixed-Stack Cocrystals Based on Perylene Diimide toward Ambipolar Charge Transport.

Small 2021 Apr 6:e2006574. Epub 2021 Apr 6.

Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Sciences Tianjin University, Collaborative Innovation Center of Chemical Science, and Engineering, Tianjin, 300072, China.

There is very limited repertoire of organic ambipolar semiconductors to date. Electron donor-acceptor alternative stacking is a unique and important binary motif for 1D mixed-stack cocrystals, opening up possibilities for the development of organic ambipolar semiconductors. Herein, four 1D mixed-stack cocrystals using N,N'-bis(perfluorobutyl)-1,7-dicyanoperylene-3,4:9,10-bis(dicarboximide) (PDICNF) as the acceptor and anthracene, pyrene, perylene, and meso-diphenyl tetrathia[22]annulene[2,1,2,1] (DPTTA) as the donors are achieved in a stoichiometric ratio (D:A = 1:1) through solution or vapor processed methods. Their packing structures, energy levels, charge transfer interactions, coassembling behaviors, and molecular orientations are systematically investigated by single-crystal X-ray analysis, absorption spectra, fluorescence quenching, Job's curve plot, and polarized photoluminescence measurements with the help of theoretical calculations. The donor-acceptor alternative stacking direction coincides with the long axis for all the four cocrystals. The field-effect transistors based on Pyrene-PDICNF show the electron mobility up to 0.19 cm V s , which is the highest value among perylene diimide-based cocrystals. Moreover, DPTTA-PDICNF cocrystals possess well-balanced electron and hole mobility with 1.7 × 10 and 2.0 × 10  cm V s respectively due to both hole and electron strong superexchange interactions, shedding light on the design of 1D mixed-stack cocrystals with excellent ambipolar transport behaviors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202006574DOI Listing
April 2021

Sub-thermionic, ultra-high-gain organic transistors and circuits.

Nat Commun 2021 03 26;12(1):1928. Epub 2021 Mar 26.

National Laboratory of Solid-State Microstructures, School of Electronic Science and Engineering and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, 210093, China.

The development of organic thin-film transistors (OTFTs) with low power consumption and high gain will advance many flexible electronics. Here, by combining solution-processed monolayer organic crystal, ferroelectric HfZrO gating and van der Waals fabrication, we realize flexible OTFTs that simultaneously deliver high transconductance and sub-60 mV/dec switching, under one-volt operating voltage. The overall optimization of transconductance, subthreshold swing and output resistance leads to transistor intrinsic gain and amplifier voltage gain over 5.3 × 10 and 1.1 × 10, respectively, which outperform existing technologies using organics, oxides and low-dimensional nanomaterials. We further demonstrate battery-powered, integrated wearable electrocardiogram (ECG) and pulse sensors that can amplify human physiological signal by 900 times with high fidelity. The sensors are capable of detecting weak ECG waves (undetectable even by clinical equipment) and diagnosing arrhythmia and atrial fibrillation. Our sub-thermionic OTFT is promising for battery/wireless powered yet performance demanding applications such as electronic skins and radio-frequency identification tags, among many others.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-22192-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997979PMC
March 2021

Porcine hemagglutinating encephalomyelitis virus induces atypical autophagy via opposite regulation of expression and nuclear translocation of transcription factor EB.

Vet Microbiol 2021 Apr 17;255:109015. Epub 2021 Feb 17.

Key Laboratory ofZoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address:

Porcine hemagglutinating encephalomyelitis virus (PHEV) displays neurotropism and induces atypical autophagy. However, the exact mechanisms mediating autophagy induced by PHEV remains uncharacterized. Transcription factor EB (TFEB) is a master transcriptional regulator playing a key role in autophagy and its activity is regulated by MTORC1 kinase on the surface of lysosomes. We first found that PHEV infection decreases TFEB expression, while it activates TFEB by inhibiting MTORC1 activation, indicating that TFEB plays a complex role in the process of PHEV-induced autophagy through opposite regulation of its expression and activity. Furthermore, this study preliminarily demonstrated that PHEV replication is dependent on TFEB expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vetmic.2021.109015DOI Listing
April 2021

Nonverbal Behaviors "Speak" Relational Messages of Dominance, Trust, and Composure.

Front Psychol 2021 26;12:624177. Epub 2021 Jan 26.

Department of Communication, UC Santa Barbara, Santa Barbara, CA, United States.

Nonverbal signals color the meanings of interpersonal relationships. Humans rely on facial, head, postural, and vocal signals to express relational messages along continua. Three of relevance are dominance-submission, composure-nervousness and trust-distrust. Machine learning and new automated analysis tools are making possible a deeper understanding of the dynamics of relational communication. These are explored in the context of group interactions during a game entailing deception. The "messiness" of studying communication under naturalistic conditions creates many measurement and design obstacles that are discussed here. Possibilities for their mitigation are considered.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2021.624177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870468PMC
January 2021

Simultaneous determination of five antiviral drug residues and stability studies in honey using a two-step fraction capture coupled to liquid chromatography tandem mass spectrometry.

J Chromatogr A 2021 Feb 8;1638:461890. Epub 2021 Jan 8.

Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Laboratory of Risk Assessment for Quality and Safety of Bee Products, Ministry of Agriculture, Beijing 100093, China; Key Laboratory of Bee Products for Quality and Safety Control, Ministry of Agriculture, Beijing 100093, China. Electronic address:

An effective sample pretreatment method followed by liquid chromatography tandem mass spectrometry (LC-MS/MS) was first developed for simultaneous determination of five antiviral drug residues including ribavirin, moroxydine, amantadine, rimantadine and memantine in honey. To adsorb analytes with different binding properties and overcome the interference of sugars and uridine as endogenous ribavirin structural analogs in honey, the target drugs were extracted with 1% formic acid and then purified by a phenylboronic acid (PBA) solid phase extraction cartridge using two-step fraction capture prior to LC-MS/MS analysis. This method was validated by analyzing honey samples from nine floral origins including miscellaneous flowers, citrus, vitex, rape, acacia, sunflower, linden, buckwheat and jujube spiking at multiple levels, and the recoveries ranged from 82.46% to 116.34%, with relative standard deviations (RSDs) less than 14.58%. The limits of detection (LODs) and limits of quantitation (LOQs) of moroxydine, ribavirin, amantadine, rimantadine, and memantine were 0.1-2 µg/kg and 0.2-5 µg/kg, respectively. Depletion experiments of five antiviral drugs in honey at different storage and process temperatures demonstrated that moroxydine can potentially be used as a drug to cure sacbrood disease in honeybees.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chroma.2021.461890DOI Listing
February 2021

Vibration characteristics of triple-gear-rotor system in compressed air energy storage under variable torque load.

Sci Prog 2021 Jan-Mar;104(1):36850420987058

Institute of Engineering Thermophysics, Chinese Academy of Sciences, Beijing, China.

This paper focuses on the effects of the off-design operation of CAES on the dynamic characteristics of the triple-gear-rotor system. A finite element model of the system is set up with unbalanced excitations, torque load excitations, and backlash which lead to variations of tooth contact status. An experiment is carried out to verify the accuracy of the mathematical model. The results show that when the system is subjected to large-scale torque load lifting at a high rotating speed, it has two stages of relatively strong periodicity when the torque load is light, and of chaotic when the torque load is heavy, with the transition between the two states being relatively quick and violent. The analysis of the three-dimensional acceleration spectrum and the meshing force shows that the variation in the meshing state and the fluctuation of the meshing force is the basic reasons for the variation in the system response with the torque load. In addition, the three rotors in the triple-gear-rotor system studied show a strong similarity in the meshing states and meshing force fluctuations, which result in the similarity in the dynamic responses of the three rotors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0036850420987058DOI Listing
January 2021

Efflux identification and engineering for ansamitocin P-3 production in Actinosynnema pretiosum.

Appl Microbiol Biotechnol 2021 Jan 4;105(2):695-706. Epub 2021 Jan 4.

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Ansamitocin P-3 (AP-3) exhibits potent biological activities against various tumor cells. As an important drug precursor, reliable supply of AP-3 is limited by low fermentation yield. Although different strategies have been implemented to improve AP-3 yield, few have investigated the impact of efflux on AP-3 production. In this study, AP-3 efflux genes were identified through combined analysis of two sets of transcriptomes. The production-based transcriptome was implemented to search for efflux genes highly expressed in response to AP-3 accumulation during the fermentation process, while the resistance-based transcriptome was designed to screen for genes actively expressed in response to the exogenous supplementation of AP-3. After comprehensive analysis of two transcriptomes, six efflux genes outside the ansamitocin BGC were identified. Among the six genes, individual deletion of APASM_2704, APASM_6861, APASM_3193, and APASM_2805 resulted in decreased AP-3 production, and alternative overexpression led to AP-3 yield increase from 264.6 to 302.4, 320.4, 330.6, and 320.6 mg/L, respectively. Surprisingly, APASM_2704 was found to be responsible for exportation of AP-3 and another macro-lactam antibiotic pretilactam. Furthermore, growth of APASM_2704, APASM_3193, or APASM_2805 overexpression mutants was obviously improved under 300 mg/L AP-3 supplementation. In summary, our study has identified AP-3 efflux genes outside the ansamitocin BGC by comparative transcriptomic analysis, and has shown that enhancing the transcription of transporter genes can improve AP-3 production, shedding light on strategies used for exporter screening and antibiotic production improvement. KEY POINTS: • AP-3-related efflux genes were identified by transcriptomic analysis. • Deletion of the identified efflux genes led in AP-3 yield decrease. • Overexpression of the efflux genes resulted in increased AP-3 production.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00253-020-11044-6DOI Listing
January 2021

Analysis of the Stable Interphase Responsible for the Excellent Electrochemical Performance of Graphite Electrodes in Sodium-Ion Batteries.

Small 2020 Dec 27;16(51):e2003268. Epub 2020 Nov 27.

Chemical Sciences and Engineering Division, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL, 60439, USA.

Considerable efforts have been exerted to understand the formation and properties of the solid electrolyte interphase (SEI) in sodium ion batteries. However, the puzzling existence and role of SEI behind the huge volume changes of the graphite electrodes need to be answered. Herein, the reason of how ether-derived SEI maintains excellent reversibility despite the huge volume changes during cycling is unraveled. Theoretical simulations and Fourier-transform infrared spectroscopy demonstrate the formation mechanism of an SEI between the graphite anode and electrolyte. Furthermore, the high mechanical tolerance of the ether-derived SEI is confirmed in atomic force microscopy. A depth profile of X-ray photoelectron spectroscopy points to a multilayer structure of the ether-derived SEI. The outer layer comprises organics (sodium alkoxide), while the inorganics (Na CO , NaF) in interior region are mixed with some organics. Notably, the presence of organics ensures the adaptability of the SEI to the volume expansion of graphite during cycling, and the concentrated distribution of inorganics improves the Young's modulus (resistance to deformation). Therefore, the graphite anode exhibits high cycle stability (96.6% capacity retention ratio at 1 A g over 860 cycles) and efficiency (≈99.5%).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202003268DOI Listing
December 2020

Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity.

Cell Death Dis 2020 11 12;11(11):975. Epub 2020 Nov 12.

Cancer Institute of Traditional Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 200032, Shanghai, China.

Platinum is a widely used first-line chemotherapy in treating non-small cell lung cancer of adenocarcinoma. Unfortunately, platinum resistance leads to relapse and therapeutic failure, enabling the development of platinum-sensitization strategies to be of great clinical significance. Here, we report that the upregulation of the NEDD8-conjugating enzyme UBE2F is an important way for lung cancer cells to escape platinum-induced cell apoptosis, which confers to insensitivity to platinum-based chemotherapy. Mechanistically, platinum treatment impairs the complex formation for proteasome-mediated UBE2F degradation, evidenced by the weaker association between UBE2F and Ring-box protein 1 (RBX1), an essential component of Cullin-Ring E3 ligases (CRLs), thus leading to the accumulation of UBE2F. The accumulated UBE2F promotes the neddylation levels and activity of Cullin5, in accord with the lower expression of pro-apoptotic protein NOXA, a well-known substrate of Cullin-Ring E3 ligase 5 (CRL5). Additionally, knockout of UBE2F significantly sensitizes lung cancer cells to platinum treatment by enhancing the protein levels of NOXA and subsequently promoting cell apoptosis. Our observations uncover a previously unknown regulatory mechanism of UBE2F stability upon platinum chemotherapy and suggest that UBE2F might be a novel therapy target for sensitizing lung cancer cells to platinum-based chemotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-020-03184-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665193PMC
November 2020

Reducing CO to HCO at Mild Potentials: Lessons from Formate Dehydrogenase.

J Am Chem Soc 2020 11 3;142(46):19438-19445. Epub 2020 Nov 3.

Department of Chemistry, University of California, Irvine, California 92697, United States.

The catalytic reduction of CO to HCO requires a formal transfer of a hydride (two electrons, one proton). Synthetic approaches for inorganic molecular catalysts have exclusively relied on classic metal hydrides, where the proton and electrons originate from the metal (via heterolytic cleavage of an M-H bond). An analysis of the scaling relationships that exist in classic metal hydrides reveal that hydride donors sufficiently hydridic to perform CO reduction are only accessible at very reducing electrochemical potentials, which is consistent with known synthetic electrocatalysts. By comparison, the formate dehydrogenase enzymes operate at relatively mild potentials. In contrast to reported synthetic catalysts, none of the major mechanistic proposals for hydride transfer in formate dehydrogenase proceed through a classic metal hydride. Instead, they invoke formal hydride transfer from an orthogonal or bidirectional mechanism, where the proton and electrons are not colocated. We discuss the thermodynamic advantages of this approach for favoring CO reduction at mild potentials, along with guidelines for replicating this strategy in synthetic systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.0c07965DOI Listing
November 2020

Design and Analysis of a Variable Inertia Spatial Robotic Tail for Dynamic Stabilization.

Biomimetics (Basel) 2020 Oct 25;5(4). Epub 2020 Oct 25.

Mechanical Engineering, Robotics and Mechatronics Lab, Virginia Tech, Blacksburg, VA 24060, USA.

This paper presents the design of a four degree-of-freedom (DoF) spatial tail and demonstrates the dynamic stabilization of a bipedal robotic platform through a hardware-in-loop simulation. The proposed tail design features three active revolute joints with an active prismatic joint, the latter of which provides a variable moment of inertia. Real-time experimental results validate the derived mathematical model when compared to simulated reactive moment results, both obtained while executing a pre-determined trajectory. A 4-DoF tail prototype was constructed and the tail dynamics, in terms of reactive force and moments, were validated using a 6-axis load cell. The paper also presents a case study where a zero moment point (ZMP) placement-based trajectory planner, along with a model-based controller, was developed in order for the tail to stabilize a simulated unstable biped robot. The case study also demonstrates the capability of the motion planner and controller in reducing the system's kinetic energy during periods of instability by maintaining ZMP within the support polygon of the host biped robot. Both experimental and simulation results show an improvement in the tail-generated reactive moments for robot stabilization through the inclusion of prismatic motion while executing complex trajectories.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/biomimetics5040055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709705PMC
October 2020

The innovative fabrication of nano-natural antimicrobial [email protected] microgels-TiO hybrid films capable of absorbing UV and antibacterial on touch screen panel.

Colloids Surf B Biointerfaces 2021 Jan 17;197:111410. Epub 2020 Oct 17.

College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address:

It is significant to develop a protective film (coating) for touch screen panels with combined absorbing UV and antibacterial performances. This work developed a smart strategy to fabricate multifunctional protective films for touch screen panels. Polymeric microgels of polyquaternium-10 (PQ) and sodium alginate (SA) were firstly fabricated based on electrostatic interactions. The PQ-SA microgels solution was then cast on a glass substrate to produce PQ-SA films. Further, peroxo titanic acid as cross-linking agent was employed to cross link PQ-SA films via forming coordination bonds between polymers and Ti of peroxo titanic acid, whereafter TiO nanoparticles were generated in situ in PQ-SA films by hydrolyzing the peroxo titanic acid to endow PQ-SA films with excellent absorbing UV ability. Moreover, water-soluble quercetin and resveratrol nanoparticles are fabricated by incorporating solvent exchange method and borate esterification reaction and were loaded into the PQ-TiO-SA films to give the films good antibacterial performance. The as-prepared multifunctional films not only exhibited excellent absorbing UV, filtering visible light and antibacterial properties, but also possessed good water resistance, abrasion resistance, self-healing performances and high adhesion to a glass substrate. We believe that present multifunctional film as a protective film for touch screen panels can provide protection for users.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.colsurfb.2020.111410DOI Listing
January 2021

Light-modulated vertical heterojunction phototransistors with distinct logical photocurrents.

Light Sci Appl 2020 23;9:167. Epub 2020 Sep 23.

School of Optoelectronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu, 610054 China.

The intriguing carrier dynamics in graphene heterojunctions have stimulated great interest in modulating the optoelectronic features to realize high-performance photodetectors. However, for most phototransistors, the photoresponse characteristics are modulated with an electrical gate or a static field. In this paper, we demonstrate a graphene/C/pentacene vertical phototransistor to tune both the photoresponse time and photocurrent based on light modulation. By exploiting the power-dependent multiple states of the photocurrent, remarkable logical photocurrent switching under infrared light modulation occurs in a thick C layer (11 nm) device, which implies competition of the photogenerated carriers between graphene/C and C/pentacene. Meanwhile, we observe a complete positive-negative alternating process under continuous 405 nm irradiation. Furthermore, infrared light modulation of a thin C (5 nm) device results in a photoresponsivity improvement from 3425 A/W up to 7673 A/W, and we clearly probe the primary reason for the distinct modulation results between the 5 and 11 nm C devices. In addition, the tuneable bandwidth of the infrared response from 10 to 3 × 10 Hz under visible light modulation is explored. Such distinct types of optical modulation phenomena and logical photocurrent inversion characteristics pave the way for future tuneable logical photocurrent switching devices and high-performance phototransistors with vertical graphene heterojunction structures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41377-020-00406-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509774PMC
September 2020

Lipidomic Biomarkers of Extracellular Vesicles for the Prediction of Preterm Birth in the Early Second Trimester.

J Proteome Res 2020 10 22;19(10):4104-4113. Epub 2020 Sep 22.

BGI-Shenzhen, Shenzhen 518083, China.

Preterm birth is the leading cause of infant death worldwide and results in a high societal economic burden associated with newborn care. Recent studies have shown that extracellular vesicles (EVs) play an important role in fetal development during pregnancy. Lipids in EVs related to preterm birth remain undefined. Here, we fully investigated differences in lipids in plasma, microvesicles (MVs), and exosomes (Exos) between 27 preterm and 66 full-term pregnant women in the early second trimester (12-24 weeks) using an untargeted lipidomics approach. Independent of other characteristics of samples, we detected 97, 58, and 10 differential features (retention time (RT) and /) with identification in plasma, MVs, and Exos, respectively. A panel of five lipids from MVs has an area under the receiver operating characteristic curve (AUC) of 0.87 for the prediction of preterm birth. One lipid of the panel (PS (34:0)) was validated in an additional 83 plasma samples (41 preterm and 42 full-term deliveries) by the pseudotargeted lipidomics method (AUC = 0.71). Our results provide useful information about the early prediction of preterm birth, as well as a better understanding of the underlying mechanisms and intervention of preterm birth. The MS data have been deposited in the CNSA (https://db.cngb.org/cnsa/) of CNGBdb with accession code CNP0001076.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jproteome.0c00525DOI Listing
October 2020

An Experimental Model of Neurodegenerative Disease Based on Porcine Hemagglutinating Encephalomyelitis Virus-Related Lysosomal Abnormalities.

Mol Neurobiol 2020 Dec 2;57(12):5299-5306. Epub 2020 Sep 2.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Lysosomes are involved in pathogenesis of a variety of neurodegenerative diseases and play a large role in neurodegenerative disorders caused by virus infection. However, whether virus-infected cells or animals can be used as experimental models of neurodegeneration in humans based on virus-related lysosomal dysfunction remain unclear. Porcine hemagglutinating encephalomyelitis virus displays neurotropism in mice, and neural cells are its targets for viral progression. PHEV infection was confirmed to be a risk factor for neurodegenerative diseases in the present. The findings demonstrated for the first time that PHEV infection can lead to lysosome disorders and showed that the specific mechanism of lysosome dysfunction is related to PGRN expression deficiency and indicated similar pathogenesis compared with human neurodegenerative diseases upon PHEV infection. Trehalose can also increase progranulin expression and rescue abnormalities in lysosomal structure in PHEV-infected cells. In conclusion, these results suggest that PHEV probably serve as a disease model for studying the pathogenic mechanisms and prevention of other degenerative diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12035-020-02105-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463228PMC
December 2020

Rational Tuning of a LiSiO-Based Hybrid Interface with Unique Stepwise Prelithiation for Dendrite-Proof and High-Rate Lithium Anodes.

ACS Appl Mater Interfaces 2020 Sep 21;12(35):39362-39371. Epub 2020 Aug 21.

Beijing Key Laboratory of Environmental Science and Engineering, School of Materials Science & Engineering, Beijing Institute of Technology, Beijing 100081, China.

Lithium metal batteries (LMBs) are among the most promising candidates for high energy-density batteries. However, dendrite growth constitutes the biggest stumbling block to its development. Herein, LiSiO-dominating organic-inorganic hybrid layers are rationally designed by SiO surface modification and the stepwise prelithiation process. SiO nanoparticles construct a zigzagged porous structure, where a solid electrolyte interface (SEI) has grown and penetrated to form a conformal and compact hybrid surface. Such a first-of-this-kind structure enables enhanced Li dendrite prohibition and surface stability. The interfacial chemistry reveals a two-step prelithiation process that transfers SiO into well-defined LiSiO, the components of which exhibits the lowest diffusion barrier (0.12 eV atom) among other highlighted SEI species, such as LiF (0.175 eV atom) for the current artificial layer. Therefore, the decorated Li allows for an improved high-rate full-cell performance (LiFePO/modified Li) with a much higher capacity of 65.7 mAh g at 5C (1C = 170 mAh g) than its counterpart with bare Li (∼3 mAh g). Such a protocol provides insights into the surface architecture and SEI component optimization through prelithiation in the target of stable, dendrite-proof, homogenized Li solid-state migration and high electrochemical performance for LMBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c12514DOI Listing
September 2020

Annexin A5 is essential for PKCθ translocation during T-cell activation.

J Biol Chem 2020 10 12;295(41):14214-14221. Epub 2020 Aug 12.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China

T-cell activation is a critical part of the adaptive immune system, enabling responses to foreign cells and external stimulus. In this process, T-cell antigen receptor (TCR) activation stimulates translocation of the downstream kinase PKCθ to the membrane, leading to NF-κB activation and thus transcription of relevant genes. However, the details of how PKCθ is recruited to the membrane remain enigmatic. It is known that annexin A5 (ANXA5), a calcium-dependent membrane-binding protein, has been reported to mediate PKCδ activation by interaction with PKCδ, a homologue of PKCθ, which implicates a potential role of ANXA5 involved in PKCθ signaling. Here we demonstrate that ANXA5 does play a critical role in the recruitment of PKCθ to the membrane during T-cell activation. ANXA5 knockout in Jurkat T cells substantially inhibited the membrane translocation of PKCθ upon TCR engagement and blocked the recruitment of CARMA1-BCL10-MALT1 signalosome, which provides a platform for the catalytic activation of IKKs and subsequent activation of canonical NF-κB signaling in activated T cells. As a result, NF-κB activation was impaired in ANXA5-KO T cells. T-cell activation was also suppressed by ANAX5 knockdown in primary T cells. These results demonstrated a novel role of ANXA5 in PKC translocation and PKC signaling during T-cell activation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1074/jbc.RA120.015143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549025PMC
October 2020

Observation of Strong -Aggregate Light Emission in Monolayer Molecular Crystal on Hexagonal Boron Nitride.

J Phys Chem A 2020 Sep 6;124(37):7340-7345. Epub 2020 Sep 6.

Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, P. R. China.

aggregates are widely used in studies of light-matter interaction and organic optoelectronic devices. Although -aggregate films can be fabricated on salt by epitaxial growth method, the size is limited to hundreds of nanometer. In this work, with hexagonal boron nitride (h-BN) as a substrate, highly crystalline -aggregate ultrathin films of ,'-ditridecylperylene 3,4,9,10-tetracarboxylic diimide (PTCDI-C) are achieved by physical vapor transport (PVT) method. Significant bathochromically shifted absorption band and narrowed 0-0 transition are observed in the monolayer PTCDI-C crystal on h-BN. The exciton coherence number of monolayer aggregate film extracted from the photoluminescence (PL) spectrum is up to 15 at = 140 K, which is higher than that of the epitaxially grown layer on salt. Beyond the first molecular layer, the multilayer crystal on h-BN is dominated by -aggregates. Further study shows that that the first molecular layer on h-BN adopts the highly ordered face-on configuration, while the overlayers adopt the edge-on motif. As a comparison, only aggregate PTCDI-C ultrathin films are found on SiO substrates, but no -aggregates. The results suggest that high-quality -aggregates can be prepared by utilizing appropriate substrates via physical vapor transport.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpca.0c03709DOI Listing
September 2020

Serum immunoglobulin G N-glycome: a potential biomarker in endometrial cancer.

Ann Transl Med 2020 Jun;8(12):748

Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: With the increase in incidence and mortality of endometrial cancer (EC), there is an urgent need to explore non-invasive strategies for identifying EC patients and facilitating risk stratification. The alteration of immunoglobulin G (IgG) N-glycome has been indicated in autoimmune diseases and several cancer types, demonstrating a significant association with disease pathogenesis and progression. However, little has been investigated in the IgG N-glycome of EC patients.

Methods: A total of 94 EC patients and 112 healthy females were recruited and sorted into an EC cohort and a control cohort. Serum samples were obtained from every participant, and IgG N-glycome profiling was conducted using ultra-performance liquid chromatography (UPLC).

Results: A total of 24 directly measured N-glycans and 11 derived traits based on the shared glycan structures were analyzed in the EC and control cohorts. We detected a significant downregulation of galactosylation and sialylation in the EC cohort compared with the control cohort, while glycans with bisecting N-acetylglucosamine (GlcNAc) were elevated in EC patients. Receiver operating characteristic (ROC) analysis based on glycan traits showed good diagnostic performance of IgG N-glycans for EC. Furthermore, by exploring the association of IgG N-glycome with prognostic risk factors in EC, we observed that lower levels of galactosylation and sialylation were correlated with high-risk factors including older age, non-endometrioid histologic subtypes, advanced stage, poor differentiation of tumor, and >50% myometrial invasion (MI).

Conclusions: Our results suggest that the IgG N-glycome profile could be a potential biomarker for EC diagnosis and a promising indicator for prognostic risk factors, and thus may facilitate the early detection of EC and the identification of high-risk patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-3504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333120PMC
June 2020

Pressure Effect on Electronic and Excitonic Properties of Purely J-Aggregated Monolayer Organic Semiconductor.

J Phys Chem Lett 2020 Aug 13;11(15):5896-5901. Epub 2020 Jul 13.

Key Laboratory of Flexible Electronics & Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, China.

Different from monolayer inorganic semiconductors, such as transition metal dichalcogenides, monolayer organic semiconductors derived from perylene have attracted much attention because of their strong absorption and bright photoluminescence (PL). Pressure has proved to be an effective tool in probing the exciton behavior in monolayer semiconductors. Here, by studying the high-pressure behavior of purely J-aggregated monolayer organic semiconductors experimentally and theoretically, we find a red shift of PL spectra due to a decrease of band gap, which is consistent with fluorescent images taken under pressure. The PL center dominates the perylene group and the band edges are flat, indicating Frenkel exciton in the monolayer organic semiconductor under ambient conditions. With increasing pressure, the band edges become more dispersive, suggesting the exciton transform to Wannier-Mott exciton, which is commonly observed in inorganic semiconductors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpclett.0c01809DOI Listing
August 2020

cGAS-STING-mediated DNA sensing maintains CD8 T cell stemness and promotes antitumor T cell therapy.

Sci Transl Med 2020 06;12(549)

Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Gynecologic Oncology, Ren Ji Hospital and Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Although cGAS-STING-mediated DNA sensing in tumor cells or phagocytes is central for launching antitumor immunity, the role of intrinsic cGAS-STING activation in T cells remains unknown. Here, we observed that peripheral blood CD8 T cells from patients with cancer showed remarkably compromised expression of the cGAS-STING cascade. We demonstrated that the cGAS-STING cascade in adoptively transferred CD8 T cells was essential for antitumor immune responses in the context of T cell therapy in mice. Mechanistically, cell-autonomous cGAS and STING promoted the maintenance of stem cell-like CD8 T cells, in part, by regulating the transcription factor TCF1 expression. Moreover, autocrine cGAS-STING-mediated type I interferon signaling augmented stem cell-like CD8 T cell differentiation program mainly by restraining Akt activity. In addition, genomic DNA was selectively enriched in the cytosol of mouse CD8 T cells upon in vitro and in vivo stimulation. STING agonism enhanced the formation of stem-like central memory CD8 T cells from patients with cancer and potentiated antitumor responses of CAR-T cell therapy in a xenograft model. These findings advance our understanding of inherent cGAS-STING activation in T cells and provide insight into the development of improved T cell therapy by harnessing the cGAS-STING pathway for cancer immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/scitranslmed.aay9013DOI Listing
June 2020

The Antitumor Agent Ansamitocin P-3 Binds to Cell Division Protein FtsZ in .

Biomolecules 2020 04 30;10(5). Epub 2020 Apr 30.

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Ansamitocin P-3 (AP-3) is an important antitumor agent. The antitumor activity of AP-3 is a result of its affinity towards β-tubulin in eukaryotic cells. In this study, in order to improve AP-3 production, the reason for severe growth inhibition of the AP-3 producing strain WXR-24 under high concentrations of exogenous AP-3 was investigated. The cell division protein FtsZ, which is the analogue of β-tubulin in bacteria, was discovered to be the AP-3 target through structural comparison followed by a SPR biosensor assay. AP-3 was trapped into a less hydrophilic groove near the GTPase pocket on FtsZ by hydrogen bounding and hydrophobic interactions, as revealed by docking analysis. After overexpression of the gene coding for FtsZ in WXR-30, the resistance to AP-3 was significantly improved. Moreover, AP-3 yield was increased from 250.66 mg/L to 327.37 mg/L. After increasing the concentration of supplemented yeast extract, the final yield of AP-3 reached 371.16 mg/L. In summary, we demonstrate that the cell division protein FtsZ is newly identified as the bacterial target of AP-3, and improving resistance is an effective strategy to enhance AP-3 production.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/biom10050699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277737PMC
April 2020