Publications by authors named "Xinping Wang"

205 Publications

High-Performance Flexible Asymmetric Supercapacitor Paired with [email protected] Heterojunctions and MXene Electrodes.

ACS Appl Mater Interfaces 2021 Sep 24;13(35):41537-41544. Epub 2021 Aug 24.

Key Laboratory of Surface & Interface Science of Polymer Materials of Zhejiang Province, Department of Chemistry, Zhejiang Sci-Tech University, 928 Second Street, Hangzhou 310018, China.

The energy density formula illuminated that widening the voltage window and maximizing capacitance are effective strategies to boost the energy density of supercapacitors. However, aqueous electrolyte-based devices generally afford a voltage window less than 1.2 V in view of water electrolysis, and chemically converted graphene yields mediocre capacitance. Herein, multi-electron redox-reversible, structurally stable indanthrone (IDT) π-backbones were rationally coupled with the reduced graphene oxide (rGO) framework to form [email protected] molecular heterojunctions. Such conductive agent- and binder-free film electrodes delivered a maximized capacitance of up to 345 F g in a potential range of -0.2 to 1.0 V. The partner film electrode-TiCT MXene which worked in the negative potential range of -0.1 to -0.6 V-afforded a capacitance as large as 769 F g. Thanks to the perfect complementary potentials of the [email protected] heterojunction positive electrode and TiCT MXene negative partner, the polyvinyl alcohol/HSO hydrogel electrolyte-based flexible asymmetric supercapacitor delivered an enlarged voltage window of 1.6 V and an impressive energy density of 17 W h kg at a high power density of 8 kW kg, plus remarkable rate capability and cycling life (capacitance retention of ∼90% after 10000 cycles) as well as exceptional flexibility and bendability.
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http://dx.doi.org/10.1021/acsami.1c08406DOI Listing
September 2021

Factor Analysis of Metabolic Syndrome and Its Relationship with the Risk of Cardiovascular Disease in Ethnic Populations in Rural Xinjiang, China.

Int J Gen Med 2021 10;14:4317-4325. Epub 2021 Aug 10.

Department of Public Health, Shihezi University School of Medicine, Shihezi, Xinjiang, People's Republic of China.

Background: This cohort study created a risk equation of CVD for the Uyghur and Kazakh ethnic groups with metabolic syndrome (MetS) in Xinjiang and its associated factors, evaluated the model's feasibility, and provided theoretical support for the prevention and early diagnosis of CVD.

Methods: A total of 5655 participants from Xinyuan and Jiashi counties in Xinjiang from 2010 to 2012 were selected, including 3770 and 1885 training and validation samples, respectively. A factor analysis was performed on 975 patients with MetS in the training sample, whereas potential factors related to CVD were extracted from 21 MetS biomarkers. Cox regression was used to create and verify a CVD-risk prediction model based on training samples. The receiver operating characteristic curve was drawn to evaluate the model's prediction efficiency.

Results: The cumulative incidence of CVD was 9.20% (training sample, 9.12%; validation sample, 9.36%). Nine potential factors were extracted from the training sample population with MetS to predict the CVD risk: lipid (hazard ratio [HR], 1.205), obesity (HR, 1.047), liver function (HR, 1.042), myocardial enzyme (HR, 1.008), protein (HR, 1.024), blood pressure (HR, 1.027), liver enzyme (HR, 1.012), renal metabolic (HR, 1.015), and blood glucose (HR, 1.010). The area under the curve of the training and validation samples was 0.841 (95% confidence interval [CI], 0.821-0.861) and 0.889 (95% CI, 0.870-0.909), respectively.

Conclusion: The CVD prediction model created with nine potential factors in patients with MetS in Kazakh and Uyghur has a good predictive power.
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http://dx.doi.org/10.2147/IJGM.S319605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364390PMC
August 2021

Mobility gradients yield rubbery surfaces on top of polymer glasses.

Nature 2021 Aug 18;596(7872):372-376. Epub 2021 Aug 18.

Department of Chemistry, Key Laboratory of Surface & Interface Science of Polymer Materials of Zhejiang Province, National Engineering Lab for Textile Fiber Materials and Processing Technology (Zhejiang), Zhejiang Sci-Tech University, Hangzhou, China.

Many emerging materials, such as ultrastable glasses of interest for phone displays and OLED television screens, owe their properties to a gradient of enhanced mobility at the surface of glass-forming liquids. The discovery of this surface mobility enhancement has reshaped our understanding of the behaviour of glass formers and of how to fashion them into improved materials. In polymeric glasses, these interfacial modifications are complicated by the existence of a second length scale-the size of the polymer chain-as well as the length scale of the interfacial mobility gradient. Here we present simulations, theory and time-resolved surface nano-creep experiments to reveal that this two-scale nature of glassy polymer surfaces drives the emergence of a transient rubbery, entangled-like surface behaviour even in polymers comprised of short, subentangled chains. We find that this effect emerges from superposed gradients in segmental dynamics and chain conformational statistics. The lifetime of this rubbery behaviour, which will have broad implications in constraining surface relaxations central to applications including tribology, adhesion, and surface healing of polymeric glasses, extends as the material is cooled. The surface layers suffer a general breakdown in time-temperature superposition (TTS), a fundamental tenet of polymer physics and rheology. This finding may require a reevaluation of strategies for the prediction of long-time properties in polymeric glasses with high interfacial areas. We expect that this interfacial transient elastomer effect and TTS breakdown should normally occur in macromolecular systems ranging from nanocomposites to thin films, where interfaces dominate material properties.
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http://dx.doi.org/10.1038/s41586-021-03733-7DOI Listing
August 2021

A high-spin diradical dianion and its bridged chemically switchable single-molecule magnet.

Chem Sci 2021 Jul 23;12(29):9998-10004. Epub 2021 Jun 23.

State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University Nanjing 210023 China

Triplet diradicals have attracted tremendous attention due to their promising application in organic spintronics, organic magnets and spin filters. However, very few examples of triplet diradicals with singlet-triplet energy gaps (Δ ) over 0.59 kcal mol (298 K) have been reported to date. In this work, we first proved that the dianion of 2,7-di--butyl-pyrene-4,5,9,10-tetraone (2,7-Bu-PTO) was a triplet ground state diradical in the magnesium complex with a singlet-triplet energy gap Δ = 0.94 kcal mol (473 K). This is a rare example of stable diradicals with singlet-triplet energy gaps exceeding the thermal energy at room temperature (298 K). Moreover, the iron analog containing the 2,7-Bu-PTO diradical dianion was isolated, which was the first single-molecule magnet bridged by a diradical dianion. When was doubly reduced to the dianion salt , single-molecule magnetism was switched off, highlighting the importance of diradicals in single-molecule magnetism.
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http://dx.doi.org/10.1039/d1sc01932eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317668PMC
July 2021

Cardiovascular Disease Prediction by Machine Learning Algorithms Based on Cytokines in Kazakhs of China.

Clin Epidemiol 2021 9;13:417-428. Epub 2021 Jun 9.

Department of Public Health, Shihezi University School of Medicine, Shihezi, Xinjiang, People's Republic of China.

Background: Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Accurately identifying subjects at high-risk of CVD may improve CVD outcomes. We sought to systematically examine the feasibility and performance of 7 widely used machine learning (ML) algorithms in predicting CVD risks.

Methods: The final analysis included 1508 Kazakh subjects in China without CVD at baseline who completed follow-up. All subjects were randomly divided into the training set (80%) and the test set (20%). L1-penalized logistic regression (LR), support vector machine with radial basis function (SVM), decision tree (DT), random forest (RF), k-nearest neighbors (KNN), Gaussian naive Bayes (NB), and extreme gradient boosting (XGB) were employed for prediction CVD outcomes. Ten-fold cross-validation was used during model developing and hyperparameters tuning in the training set. Model performance was evaluated in the test set in light of discrimination, calibration, and clinical usefulness. RF was applied to obtain the variable importance of included variables. Twenty-two variables, including sociodemographic characteristics, medical history, cytokines, and synthetic indices, were used for model development.

Results: Among 1508 subjects, 203 were diagnosed with CVD over a median follow-up of 5.17 years. All 7 models had moderate to excellent discrimination (AUC ranged from 0.770 to 0.872) and were well calibrated. LR and SVM performed identically with an AUC of 0.872 (95% CI: 0.829-0.907) and 0.868 (95% CI: 0.825-0.904), respectively. LR had the lowest Brier score (0.078) and the highest sensitivity (97.1%). Decision curve analysis indicated that SVM was slightly better than LR. The inflammatory cytokines, such as hs-CRP and IL-6, were identified as strong predictors of CVD.

Conclusion: SVM and LR can be applied to guide clinical decision-making in the Kazakh Chinese population, and further study is required to ensure their accuracies.
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http://dx.doi.org/10.2147/CLEP.S313343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200454PMC
June 2021

Ultrastable Glassy Polymer Films with an Ultradense Brush Morphology.

ACS Nano 2021 Jun 25;15(6):9568-9576. Epub 2021 May 25.

Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States.

Glassy polymer films with extreme stability could enable major advancements in a range of fields that require the use of polymers in confined environments. Yet, from a materials design perspective, we now know that the glass transition temperature () and thermal expansion of polymer thin films can be dramatically different from those characteristics of the bulk, , exhibiting confinement-induced diminished thermal stability. Here, we demonstrate that polymer brushes with an ultrahigh grafting density, , an ultradense brush morphology, exhibit a significant enhancement in thermal stability, as manifested by an exceptionally high and low expansivity. For instance, a 5 nm thick polystyrene brush film exhibits an ∼75 K increase in and ∼90% reduction in expansivity compared to a spin-cast film of similar thickness. Our results establish how morphology can overcome confinement and interfacial effects in controlling thin-film material properties and how this can be achieved by the dense packing and molecular ordering in the amorphous state of ultradense brushes prepared by surface-initiated atom transfer radical polymerization in combination with a self-assembled monolayer of initiators.
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http://dx.doi.org/10.1021/acsnano.0c09631DOI Listing
June 2021

Yttrium germole dianion complexes with Y-Ge bonds.

Dalton Trans 2021 Apr;50(16):5552-5556

State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210023, China.

The reactions of dipotassium 3,4-dimethyl-2,5-bis(trimethylsilyl)-germole dianion K2[1] with YCl3 and Cp*YCl2 (Cp* = cyclopentadienyl) in THF at room temperature afforded the dianion salt [(K-cryptand-222)2][1-YCl3] (K2[2]) and the dimeric complex [1-Y-Cp*]2 (3), respectively. While the polymeric complex {[(1)2-Y-K(toluene)]2}n (4) was obtained from the reaction of K2[1] and half molar equivalent of YCl3(THF)3.5 in toluene at 80 °C. The germole dianions in complexes 3 and 4 feature η5/η1 coordination interactions with the yttrium atoms. They represent the first examples of rare earth (RE) complexes containing RE-Ge bonds other than the RE-GeR3 structural type.
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http://dx.doi.org/10.1039/d1dt00798jDOI Listing
April 2021

Persistent 2-3 σ-bonded heteronuclear radical cations centered on S/Se and P/As atoms.

Chem Commun (Camb) 2021 May;57(41):5067-5070

State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

The two-center three-electron (2c-3e) bonded species are important in chemical and biological science. Reported isolable 2c-3e σ-bonded species are usually constructed in homoatomic radicals. The one-electron oxidation of main-group heteronuclear species Nap(SPh)(P(Mes)2) (1), Nap(SePh)(P(Mes)2) (2), Nap(SPh)(As(Mes)2) (3) and Nap(SePh)(As(Mes)2) (4) produced persistent radical cations 1˙+-4˙+ in solution. Large couplings of heteroatoms in EPR spectra of 1˙+-4˙+, shorter bond distances and bigger Wiberg bond orders of Ch-Pn in 1˙+-4˙+ than those in 1-4 in DFT calculations indicate large amounts of spin densities over heteroatoms and the formation of 2c-3e σ-bonds between chalcogen and pnicogen atoms. This work provides evidence of 2c-3e σ-bonds constructed between main-group heteronuclears and rare examples of radical cations involving three-electron σ-bonds between S/Se and P/As atoms.
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http://dx.doi.org/10.1039/d1cc01117kDOI Listing
May 2021

Transfunctionalization of graphite fluoride engineered polyaniline grafting to graphene for High-Performance flexible supercapacitors.

J Colloid Interface Sci 2021 Sep 31;597:289-296. Epub 2021 Mar 31.

Department of Chemistry, Key Laboratory of Surface & Interface Science of Polymer Materials of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address:

Low energy density is the major obstacle for the practical all-solid-state supercapacitors, which may be raised by the combination of the pseudocapacitance with the electrochemical double-layer capacitance. Although graphene and polyaniline have been demonstrated two effective materials, the synthetic route of graphene and their hybrid mode largely dictated the capacitive performances and cyclability of graphene/polyaniline nanocomposites. Herein, we employed commercial graphite fluoride as the precursor to obtain graphene with a well-preserved carbon lattice. After graphite fluoride functionalization by p-phenylenediamine (pPDA) and in situ oxidative polymerization of anilines, polyaniline (PANI) chains were covalently attached to graphene framework through pPDA bridges. Multiple characterizations were performed to confirm the covalent binding mode between graphene scaffolds and PANI partners, and electrochemical tests unraveled the as-prepared G-pPDA-PANI triads delivered a gravimetric capacitance as high as 638F g and a further amplified volumetric capacitance (up to 759F cm). The bendable all-solid-state supercapacitors yielded an encouraging energy density of over 18 W   h L at a power density high to 5,950 W L, while exhibiting an exceptional rate capability, cycling stability and mechanical flexibility.
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http://dx.doi.org/10.1016/j.jcis.2021.03.169DOI Listing
September 2021

Factors associated with prognosis after small-vessel occlusion among young and middle-aged patients: a hospital-based follow-up study.

Postgrad Med 2021 Jun 15;133(5):581-586. Epub 2021 May 15.

Department of Neurology, Tianjin Huanhu Hospital, Tianjin China.

: Small-vessel occlusions are the most common causes of mild strokes and, in China, account for approximately 27.3% of ischemic stroke cases. However, the factors associated with short- and long-term outcomes appear contradictory. Thus, we assessed the factors related to outcomes 3 years after small-vessel occlusion among patients aged 18 to 55 years.: Between 2007 and 2014, we recruited patients who experienced small-vessel occlusion (according to Trial of Org 10,172 in Acute Stroke Treatment [TOAST] classification) aged 18 to 55 years and conducted a hospital-based follow-up study. The assessed outcomes were mortality, recurrence, and dependency within 3 years after the initial stroke. The outcome determinants were assessed using a multivariate logistic regression analysis.: A total of 276 patients (men, 76.09%) with small-vessel occlusions were enrolled in this study. In addition, 85.1% of the patients had strokes between the ages of 45 and 55 years. The risk of recurrence within 3 years was higher for patients who had moderate strokes than for those who had mild events (relative risk [RR], 3.09; 95% confidence interval [CI], 1.14-8.34; P < 0.05). Further, the risk of dependency within 3 years was 2.61 times higher in obese patients than in non-obese patients (RR, 2.61; 95% CI, 1.00-6.79; P < 0.05). The risks of recurrence and dependency within 3 years increased by 17% and 18%, respectively, for each 1-unit increase in fasting plasma glucose levels (RR, 1.17; 95% CI, 1.05-1.30 and RR, 1.18; 95% CI, 1.06-1.32, respectively; both P < 0.05).: Our findings suggest that small-vessel occlusions cause the heaviest disease burden in patients aged 45 to 55 years. To reduce stroke recurrence, young and middle-aged patients with small-vessel occlusions should control their fasting plasma glucose levels and manage their weight.
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http://dx.doi.org/10.1080/00325481.2021.1917250DOI Listing
June 2021

Surface Activity and Structure of Temperature-Responsive Polymer Surfactants Based on PNIPAm at the Air/Solution Interface.

Langmuir 2021 Apr 6;37(15):4632-4638. Epub 2021 Apr 6.

Department of Chemistry, Key Laboratory of Surface & Interface Science of Polymer Materials of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou 310018, P. R. China.

Thermally sensitive polymers have attracted tremendous interest in the design of stimulus-responsive surfactants. In this article, poly(propylene oxide)--poly(-isopropylacrylamide) (PPO--PNIPAm) with different block lengths of PNIPAm was synthesized through atom transfer radical polymerization (ATRP). Different from commercial Pluronic surfactants, four distinct sections appeared in the decrease of surface tension with concentration. First, with increasing concentration, the amount of adsorbed polymers increased and the surface tension decreased sharply until a plateau was reached, which was caused by the rearrangement of methyl groups. The increasing adsorbed amount of PPO--PNIPAm resulted in the rearrangement of isopropyl groups, which changed from a lying down or horizontal conformation to a standing up or vertical conformation. This behavior led to the decrease in surface tension in part III until the critical micelle concentration (CMC) was reached. The surface tension of PPO--PNIPAm was thermally responsive. Except for the hysteresis observed in the first cycle, the surface tension was reversible during the heating-and-cooling cycles. At low concentrations, the low surface tension at higher temperatures was mainly caused by the increasing adsorption amount and ordered arrangement of methyl groups, while the standing up conformation of isopropyl groups at higher concentrations resulted in the low surface tension observed at high temperatures.
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http://dx.doi.org/10.1021/acs.langmuir.1c00320DOI Listing
April 2021

Utilizing the Diffusion of Fluorinated Polymers to Modify the Semiconductor/Dielectric Interface in Solution-Processed Conjugated Polymer Field-Effect Transistors.

ACS Appl Mater Interfaces 2021 Feb 10;13(7):8682-8691. Epub 2021 Feb 10.

Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.

It has been demonstrated that tailoring the properties of semiconductor/dielectric interfaces with fluorinated polymers yields better performance for organic field-effect transistors (OFETs). However, it remains a challenge to fabricate bottom-gate OFET devices on fluorinated dielectrics using solution-processed methods due to the poor wettability of fluorinated dielectrics. Here, we utilized the diffusion of fluorinated poly(methyl methacrylate) (PMMA) to construct the fluorine-rich semiconductor/dielectric interface to achieve the fabrication of bottom-gate OFETs with a solution-processed poly(3-hexylthiophene) (P3HT) semiconductor layer. The consequences indicate that the fluorinated dielectrics can effectively decrease the charge traps density at the semiconductor/dielectric interface and promote the edge-on orientation of P3HT on the dielectric surface. Thus, the devices based on fluorinated PMMA modified dielectrics exhibit higher carrier mobility and electrical stability than those of the fluorine-free devices. Our investigation affords a new strategy for the design and interface optimization of devices, which may further advance the performance of OFET devices.
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http://dx.doi.org/10.1021/acsami.0c23058DOI Listing
February 2021

An evaluation of the Veterans Health Administration National Nursing Education Initiative.

Nurs Outlook 2021 Mar-Apr;69(2):193-201. Epub 2020 Dec 9.

Retired, Research and Development Service, James A Haley Veterans' Hospital and Clinics, Tampa, FL.

Background: Since 1999, the Veterans Health Administration's (VHA) National Nursing Education Initiative (NNEI) has provided 16,294 scholarships for registered nurses to attain baccalaureate and advanced nursing degrees.

Purpose: The goal of this evaluation was to determine factors that enhanced or hindered a scholarship recipient in the completion of their degree and service obligation.

Methods: A regression analysis was performed to identify predictors of degree completion for 10,043 participants in 162 VHA facilities.

Findings: Significant predictor variables for degree completion were intended occupation, entry degree, gender, age, and year entering the program. Eighty-six percent (86.7%) of participants completed the degree requirement. Of those who completed their degree, 97% completed the service obligation.

Discussion: As recruitment and retention initiative, NNEI scholarships are poised to address the VHA nursing staffing shortages as well as build a highly qualified nursing workforce capable of providing the best care to our Nation's Veterans.
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http://dx.doi.org/10.1016/j.outlook.2020.11.001DOI Listing
April 2021

Generation of a pHSPA6 gene-based multifunctional live cell sensor.

Biochim Biophys Acta Mol Cell Res 2021 02 3;1868(2):118919. Epub 2020 Dec 3.

Key Laboratory of Zoonosis Research, Ministry of Education, Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 130062 Changchun, Jilin Province, People's Republic of China. Electronic address:

Biosensors utilizing intact live cells can report responses to certain stimuli rapidly and sensitively and have attracted a great deal of attention. The expression pattern of HSPA6, a little studied HSPA family member, has contributed to the development of multifunctional and intelligent whole-cell sensors. Herein, a new pHSPA6-based EGFP fluorescent reporter cell line was designed and developed via a CRISPR/Cas9-mediated knock-in strategy. The fluorescent reporter cell line has a precise EGFP integration site and gene copy number, and no selectable marker genes were introduced during the selection processes. Stimulation experiments with HSPA6-specific stressors indicated that EGFP fluorescent reporter cells could rapidly and effectively convert stress signals into EGFP fluorescent signals. Furthermore, cell proliferation and gene expression pattern analysis showed that the fluorescent reporter cells grew well and that both the integrated EGFP gene and the pHSPA6 gene were expressed rapidly and sensitively in response to stimulation. This study provides a new strategy for the construction of a cell model for HSPA6 expression/interaction and an intelligent live cell sensor, which can potentially be applied to numerous fields, such as those focusing on cellular models of HSPA6 signaling cascades, biomaterials, food security, environmental assessment, and drug screening.
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http://dx.doi.org/10.1016/j.bbamcr.2020.118919DOI Listing
February 2021

Action of phytosterols on thermally induced trans fatty acids in peanut oil.

Food Chem 2021 May 16;344:128637. Epub 2020 Nov 16.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, PR China. Electronic address:

The effects of six phytosterols on thermally induced trans fatty acids (TFAs) in peanut oil were investigated. Peanut oil, triolein, trilinolein and trilinolenin heated at 180 °C for 12 and 24 h with or without phytosterols were analyzed by GC-FID. The atomic net charge distribution, frontier molecular orbital energy (FMOE), and bond dissociation energy (BDE) of six phytosterols were calculated by density functional theory. Results showed that six phytosterols inhibited the formation of trans oleic acid, trans linoleic acids, trans linolenic acids, and total TFAs. The anti-isomerization effects of phytosterols were mainly associated with hydroxyl site activities, which were affected by the double bond position in the main skeleton of cyclopentane tetrahydrophenanthrene and the number of double bonds on the C17 branch chain. The FMOE difference and BDE of phytosterol molecules were closely related to their anti-isomerization rates. The anti-isomerization mechanisms of phytosterols on TFAs in peanut oil were proposed.
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http://dx.doi.org/10.1016/j.foodchem.2020.128637DOI Listing
May 2021

Prevents the Recurrences of Resected Colonic Polyps by Affecting Angiogenin-2-Induced Protein Kinase B/Akt Signaling.

J Oncol 2020 28;2020:3531652. Epub 2020 Oct 28.

Department of Clinical Laboratory, Yantai Hospital of Traditional Chinese Medicine, Yantai 264002, China.

Aim: We aimed to explore the effects of Bupleuri Radix (BR) on the recurrence of resected colonic polyp (CP) by measuring angiogenin-2-induced protein kinase B (Ang PKB)/Akt signaling.

Method: The main ingredients of BR were extracted by using ethanol and measured by HPLC. One hundred twenty patients with CP >10 mm who underwent resected surgery were randomly allocated to an aspirin (AG) or a BR medicine (BG) group. The allocation ratio was 1 : 1 and the intervention duration was one year. The recurrence rate of resected CP was investigated and the plasma levels of Ang PKB/Akt and inflammatory cytokines were measured using ELISA kits. After one-year surgery, side effects were recorded. The relationship between the serum levels of the main compounds of BR and plasma levels of Ang PKB/Akt was analyzed.

Results: The main ingredients of CP are paeoniflorin, baicalin, saikosaponin A, and bupleurum saponin B2. Recurrence of resected CP was found in 17 patients from the AG group and eight patients from the BG group after one-year follow-up ( < 0.05). The levels of angiogenin-2 II and PKB/Akt in the AG group were higher than those in the BG group ( < 0.05). Meanwhile, BR treatment reduced the plasma levels of TNF-, IL-1 and IL-6, and increased the level of IL-10( < 0.05). Inflammatory cytokines are important factors that affect the recurrence of resected CP. Serum paeoniflorin, baicalin, saikosaponin A, and bupleurum saponin B2 in BR had a strong negative relationship with the plasma levels of Ang PKB/Akt.

Conclusion: BR significantly reduces the recurrence risk of resected CP by affecting Ang PKB/Akt signaling.
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http://dx.doi.org/10.1155/2020/3531652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7657685PMC
October 2020

External validation of three atherosclerotic cardiovascular disease risk equations in rural areas of Xinjiang, China.

BMC Public Health 2020 Sep 29;20(1):1471. Epub 2020 Sep 29.

Department of Public Health, Shihezi University School of Medicine, North 2th Road, Shihezi, Xinjiang, 832000, China.

Background: To externally validate the Prediction for ASCVD Risk in China (PAR) risk equation for predicting the 5-year atherosclerotic cardiovascular disease (ASCVD) risk in the Uyghur and Kazakh populations from rural areas in northwestern China and compare its performance with those of the pooled cohort equations (PCE) and Framingham risk score (FRS).

Methods: The final analysis included 3347 subjects aged 40-74 years without CVD at baseline. The 5-year ASCVD risk was calculated using the PAR, PCE, and FRS. Discrimination, calibration, and clinical usefulness of the three equations in predicting the 5-year ASCVD risk were assessed before and after recalibration.

Results: Of 3347 included subjects, 1839 were female. We observed 286 ASCVD events in within 5-year follow-up. All three risk equations had moderate discrimination in both men and women. C-indices of PAR, PCE, and FRS were 0.727 (95% CI, 0.725-0.729), 0.727 (95% CI, 0.725-0.729), and 0.740 (95% CI, 0.738-0.742), respectively, in men; the corresponding C-indices were 0.738 (95% CI, 0.737-0.739), 0.731 (95% CI, 0.730-0.732), and 0.761 (95% CI, 0.760-0.762), respectively, in women. PCE, PAR and FRS substantially underestimated the 5-year ASCVD risk in women by 70, 23 and 51%, respectively. However, PAR and FRS fairly predicted the risk in men and PAR was well calibrated. The calibrations of the three risk equations could be changed by recalibration. The decision curve analyses demonstrated that at the threshold risk of 5%, PCE was the most clinically useful in both men and women after recalibration.

Conclusions: All three risk equations underestimated the 5-year ASCVD risk in women, while PAR and FRS fairly predicted that in men. However, the results of predictive performances for three risk equations are inconsistent, more accurate risk equations are required in the primary prevention of ASCVD aiming to this Uyghur and Kazakh populations.
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http://dx.doi.org/10.1186/s12889-020-09579-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526265PMC
September 2020

Small RNA sequencing reveals a novel tsRNA-06018 playing an important role during adipogenic differentiation of hMSCs.

J Cell Mol Med 2020 11 16;24(21):12736-12749. Epub 2020 Sep 16.

Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiujiang, China.

Transfer RNA-derived small RNAs (tsRNAs), a novel type of non-coding RNA derivative, are able to regulate a wide range of biological processes. What role these tsRNAs play in the regulation of human bone marrow mesenchymal stem cell (hMSCs) adipogenic differentiation remains uncertain. We induced the adipogenic differentiation of human bone marrow mesenchymal cells (hMSCs) and then performed small RNA transcriptomic sequencing, leading us to identify tsRNA-06018 as a target of interest based upon resultant the tsRNA expression profiles. When tsRNA-06018 was knocked down, this led to the inhibition of adipogenesis and a decrease in adipogenic marker expression. When STC2 was overexpressed, this impaired the adipogenic differentiation of these cells. We further used luciferase reporter assays to confirm that tsRNA-06018 directly binds the 3'-untranslated region (3'-UTR) of STC2. In addition, we determined that both knocking down tsRNA-06018 and overexpressing STC2 increased extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation within cells. We also assessed that the adipogenic differentiation of hMSCs in which tsRNA-06018 was knocked down was further enhanced upon the addition of the ERK1/2 inhibitor U0126 as compared tsRNA-06018 knockdown alone. Taken together, using small RNA sequencing we profiled tsRNAs in hMSCs during the process of adipogenesis, leading us to identify tsRNA-06018 as a novel regulator of this differentiation process. This tsRNA was able to regulate adipogenic differentiation by targeting STC2 via the ERK1/2 signalling pathway.
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http://dx.doi.org/10.1111/jcmm.15858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686998PMC
November 2020

Zika virus NS2A protein induces the degradation of KPNA2 (karyopherin subunit alpha 2) via chaperone-mediated autophagy.

Autophagy 2020 12 28;16(12):2238-2251. Epub 2020 Sep 28.

Molecular Virology Laboratory, VA-MD College of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA.

KPNA2/importin-alpha1 (karyopherin subunit alpha 2) is the primary nucleocytoplasmic transporter for some transcription factors to activate cellular proliferation and differentiation. Aberrant increase of KPNA2 level is identified as a prognostic marker in a variety of cancers. Yet, the turnover mechanism of KPNA2 remains unknown. Here, we demonstrate that KPNA2 is degraded via the chaperone-mediated autophagy (CMA) and that Zika virus (ZIKV) enhances the KPNA2 degradation. KPNA2 contains a CMA motif, which possesses an indispensable residue Gln109 for the CMA-mediated degradation. RNAi-mediated knockdown of LAMP2A, a vital component of the CMA pathway, led to a higher level of KPNA2. Moreover, ZIKV reduced KPNA2 via the viral NS2A protein, which contains an essential residue Thr100 for inducing the CMA-mediated KPNA2 degradation. Notably, mutant ZIKV with T100A alteration in NS2A replicates much weaker than the wild-type virus. Also, knockdown of KPNA2 led to a higher ZIKV viral yield, which indicates that KPNA2 mediates certain antiviral effects. These data provide insights into the KPNA2 turnover and the ZIKV-cell interactions.
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http://dx.doi.org/10.1080/15548627.2020.1823122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751636PMC
December 2020

Local Disorder Facilitates Chain Stretching in Crowded Polymer Brushes.

J Phys Chem Lett 2020 Sep 3;11(18):7814-7818. Epub 2020 Sep 3.

Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou, 310018, China.

Intermolecular crowding of densely tethered polymers promotes chain extension and anisotropy that induces many unique properties. In this study, we used conformation-sensitive infrared spectroscopy to determine that chain extension in a polymer brush is associated with local conformation rearrangements, i.e., contraction of side groups and increased proportion of twists in the backbone, which served to increase molecular disorder at or below the segmental scale. This conformational transition points to a particular molecular mechanism for chain extension in densely tethered polymers, wherein increased local disorder facilitates global chain ordering (i.e., chain extension) and therefore supplements our current understanding of chain orientation at a molecular level.
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http://dx.doi.org/10.1021/acs.jpclett.0c02374DOI Listing
September 2020

Effect of holistic nursing intervention on postoperative rehabilitation and reduction of complications of pelvic fracture patients.

Panminerva Med 2020 Aug 5. Epub 2020 Aug 5.

Department of Rehabilitation Medicine, Yantai Hospital of Traditional Chinese Medicine, Yantai, China -

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http://dx.doi.org/10.23736/S0031-0808.20.04035-5DOI Listing
August 2020

Microarray analysis of long non-coding RNA expression profiles in low high-density lipoprotein cholesterol disease.

Lipids Health Dis 2020 Jul 28;19(1):175. Epub 2020 Jul 28.

Key Laboratory of Xinjiang Endemic and Ethnic Diseases of the Ministry of Education, Shihezi University School of Medicine, Shihezi, China.

Background: Low high-density lipoprotein cholesterol (HDL-C) disease with unknown etiology has a high prevalence in the Xinjiang Kazak population. In this study, long noncoding RNAs (lncRNAs) that might play a role in low HDL-C disease were identified.

Methods: Plasma samples from 10 eligible individuals with low HDL disease and 10 individuals with normal HDL-C levels were collected. The lncRNA profiles for 20 Xinjiang Kazak individuals were measured using microarray analysis.

Results: Differentially expressed lncRNAs and mRNAs with fold-change values not less than 1.5 and FDR-adjusted P-values less than 0.05 were screened. Bioinformatic analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and network analyses, were used to determine relevant signaling pathways and predict potential target genes. In total, 381 lncRNAs and 370 mRNAs were differentially expressed based on microarray analysis. Compared with those in healthy individuals, several lncRNAs were upregulated or downregulated in patients with low HDL-C disease, among which TCONS_00006679 was most significantly upregulated and TCONS_00011823 was most significantly downregulated. GO and KEGG pathway analyses as well as co-expression networks of lncRNAs and mRNAs revealed that the platelet activation pathway and cardiovascular disease were associated with low HDL-C disease.

Conclusions: Potential target genes integrin beta-3 (ITGB3) and thromboxane A2 receptor (TBXA2R) were regulated by the lncRNAs AP001033.3-201 and AC068234.2-202, respectively. Both genes were associated with cardiovascular disease and were involved in the platelet activation pathway. AP001033.3-201 and AC068234.2-202 were associated with low HDL-C disease and could play a role in platelet activation in cardiovascular disease. These results reveal the potential etiology of dyslipidemia in the Xinjiang Kazakh population and lay the foundation for further validation using large sample sizes.
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http://dx.doi.org/10.1186/s12944-020-01348-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388226PMC
July 2020

Characterization of the Specific Interactions between Nanoparticles and Proteins at Residue-Resolution by Alanine Scanning Mutagenesis.

ACS Appl Mater Interfaces 2020 Aug 28;12(31):34514-34523. Epub 2020 Jul 28.

Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai 200444, China.

The interaction between nanoparticles and proteins is a central problem in the nano-bio-fields. However, it is still a great challenge to characterize the specific interaction between nanoparticles and proteins in structural details. Using the Goldbodies, the artificial antibodies created by grafting complementary-determining regions (CDRs) of natural antibodies onto gold nanoparticles, as the models, we manage to identify the key residues of the CDR peptides on gold nanoparticles for the specific interactions by alanine scanning mutagenesis. Each and every residue of the CDR peptides on two Goldbodies (which specifically bind with hen egg white lysozyme and epidermal growth factor receptor, respectively) is mutated to alanine one by one, generating a total of 18 single-mutants of the two Goldbodies. Experimental results reveal that the key residues of the CDR peptides for the specific interactions between the two Goldbodies and the corresponding antigens are exactly the same as those in the natural antibodies, thus proving that the correct conformations of the CDRs of natural antibodies have been successfully reconstructed on AuNPs. This is the first residue-resolution structural illustration for the specific interaction between a designed nanoparticle and a protein.
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http://dx.doi.org/10.1021/acsami.0c05994DOI Listing
August 2020

A diradical based on odd-electron σ-bonds.

Nat Commun 2020 Jul 10;11(1):3441. Epub 2020 Jul 10.

State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, 210023, China.

The concept of odd-electron σ-bond was first proposed by Linus Pauling. Species containing such a bond have been recognized as important intermediates encountered in many fields. A number of radicals with a one-electron or three-electron σ-bond have been isolated, however, no example of a diradical based odd-electron σ-bonds has been reported. So far all stable diradicals are based on two s/p-localized or π-delocalized unpaired electrons (radicals). Here, we report a dication diradical that is based on two Se∴Se three-electron σ-bonds. In contrast, the dication of sulfur analogue does not display diradical character but exhibits a closed-shell singlet.
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http://dx.doi.org/10.1038/s41467-020-17303-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351710PMC
July 2020

Engineering with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production.

Biotechnol Biofuels 2020 15;13:108. Epub 2020 Jun 15.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062 Hubei China.

Backgrounds: Engineering yeast as a consolidated bioprocessing (CBP) microorganism by surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol production. However, most of the previous studies focused on , achieving efficient conversion of phosphoric acid-swollen cellulose (PASC) or microcrystalline cellulose (Avicel) but not carboxymethyl cellulose (CMC) to ethanol, with an average titer below 2 g/L.

Results: Harnessing an ultra-high-affinity IM7/CL7 protein pair, here we describe a method to engineer with minicellulosomes by in vitro assembly of three recombinant cellulases including an endoglucanase (EG), an exoglucanase (CBH) and a β-glucosidase (BGL), as well as a carbohydrate-binding module (CBM) on the cell surface. For the first time, the engineered yeasts enable efficient and direct conversion of CMC to bioethanol, observing an impressive ethanol titer of 5.1 g/L.

Conclusions: The research promotes the application of as a CBP cell factory in cellulosic ethanol production and provides a promising platform for screening the cellulases from different species to construct surface-assembly celluosome.
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http://dx.doi.org/10.1186/s13068-020-01749-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296672PMC
June 2020

Mechanism of blood-brain barrier disruption by an Escherichia coli from lambs with severe diarrhea and meningoencephalitis.

Microb Pathog 2020 Oct 1;147:104288. Epub 2020 Jun 1.

College of Veterinary Medicine, Jilin University, Changchun, Jilin, China; Key Laboratory for Zoonosis, Ministry of Education, Changchun, Jilin, China. Electronic address:

Escherichia coli (E. coli) is a common conditional pathogen that is associated with a variety of infections in humans and animals. Although there are increasing reports regarding the infection of E. coli to domestic animals and poultry, the infection of E. coli in lambs is relatively less reported, especially on meningoencephalitis. Here, we reported the isolation of an E. coli strain designated as NMGCF-19 from lambs characterized with severe diarrhea and neurological disorder, and demonstrated that NMGCF-19 as the causative agent has the ability to disrupt the blood-brain barrier (BBB) to cause the meningoencephalitis using a mouse model. Investigation on the mechanism regarding the NMGCF-19-related meningoencephalitis revealed a significant decreased expression of ZO-1 and occludin in mouse brain tissue in comparison with the control mice. Moreover, infection of NMGCF-19 increased the expression of proinflammatory cytokines TNF-α, IL-6, IL-1β and IL-18, up-regulated HMGB1 level, and activated TLR2/TLR4/MyD88 and NLRP3 inflammasome pathways. These findings indicated that NMGCF-19 likely invades the brain tissue by disrupting the tight junction (TJ) architecture and causes the meningoencephalitis via increasing inflammatory response and activating TLR2/TLR4/MyD88 and NLRP3 inflammasome pathways.
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http://dx.doi.org/10.1016/j.micpath.2020.104288DOI Listing
October 2020

Magnetic Multistability in an Anion-Radical Pimer.

Angew Chem Int Ed Engl 2020 Aug 8;59(33):14040-14043. Epub 2020 Jun 8.

Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Radical pimers are the simplest and most important models for studying charge-transfer processes and provide deep insight into π-stacked organic materials. Notably, radical pimer systems with magnetic bi- or multistability may have important applications in switchable materials, thermal sensors, and information-storage media. However, no such systems have been reported. Herein, we describe a new pimer consisting of neutral N-(n-propyl) benzene triimide ([BTI-3C]) and its anionic radical ([BTI-3C] ) that exhibits rare magnetic multistability. The crystalline pimer was readily synthesized by reduction of BTI-3C with cobaltocene (CoCp ). The transition occurred with a thermal hysteresis loop that was 27 K wide in the range of 170-220 K, accompanied by a smaller loop with a width of 25 K at 220-242 K. The magnetic multistability was attributed to slippage of the π-stacked BTI structures and entropy-driven conformational isomerization of the side propyl chains in the crystalline state during temperature variation.
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http://dx.doi.org/10.1002/anie.202003927DOI Listing
August 2020

Effect of metabolic syndrome on coronary heart disease in rural minorities of Xinjiang: a retrospective cohort study.

BMC Public Health 2020 Apr 25;20(1):553. Epub 2020 Apr 25.

Department of Public Health, Shihezi University School of Medicine, Shihezi, China.

Background: Metabolic syndrome is diagnosed by a cluster of risk factors that associated with an increased risk of coronary heart disease (CHD). We aimed to explore the impact of and interactions between individual metabolic syndrome components on the risk of CHD in Xinjiang.

Methods: The baseline population included 7635 participants. The degree to which the components increase the risk of CHD and the multiplicative interactions between them were assessed using hazard ratios (HRs) and 95% confidence intervals (CIs). Additive interactions were appraised by the relative excess risk due to interaction, synergy index (SI), and attributable proportion of interaction.

Results: A total of 304 CHD patients were enrolled from rural residents of Xinjiang. Elevated blood pressure (HR 1.81; 95% CI 1.35-2.44) and elevated fasting blood glucose (FBG) (HR 1.82; 95% CI 1.38-2.38) increased the risk of CHD after adjustment for confounding factors. We found a positive additive interaction (SI 1.14; 95% CI 0.51-2.51) between elevated blood pressure and elevated FBG, but none were significant. As the number of components increased, the risk of CHD increased. The combinations of [high triglycerides (TG) + low high-density lipoprotein cholesterol (HDL-C) + elevated FBG + large waistline] (HR 4.26; 95% CI 1.43-12.73) and [elevated blood pressure + elevated FBG + low HDL-C + large waistline] (HR 1.82; 95% CI 1.38-2.38) increased the risk of CHD.

Conclusions: We provide evidence that elevated blood pressure and elevated FBG are independent risk factors for CHD and it might be necessary to maintain the normal waistline for preventing CHD.
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http://dx.doi.org/10.1186/s12889-020-08612-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183686PMC
April 2020

Crystalline Diradical Dianions of Pyrene-Fused Azaacenes.

Angew Chem Int Ed Engl 2020 Jul 18;59(29):11794-11799. Epub 2020 May 18.

State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, 210023, P. R. China.

Although diradicals and azaacenes have been greatly attractive in fundamental chemistry and functional materials, the isolable diradical dianions of azaacenes are still unknown. Herein, we describe the first isolation of pyrene-fused azaacene diradical dianion salts [(18-c-6)K(THF) ] [(18-c-6)K] ⋅1 and [(18-c-6)K(THF)] ⋅2 by reduction of the neutral pyrene-fused azaacene derivatives 1 and 2 with excess potassium graphite in THF in the presence of 18-crown-6. Their electronic structures were investigated by various experiments, in conjunction with theoretical calculations. It was found that both dianions are open-shell singlets in the ground state and their triplet states are thermally readily accessible owing to the small singlet-triplet energy gap. This work provides the first examples of crystalline diradical dianions of azaacenes with considerable diradical character.
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http://dx.doi.org/10.1002/anie.202001842DOI Listing
July 2020

A Magnetically Robust Triplet Ground State Sulfur-Hydrocarbon Diradical Dication.

J Am Chem Soc 2020 Apr 13;142(16):7340-7344. Epub 2020 Apr 13.

State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210023, China.

The one- and two-electron oxidation reactions of tetrathiolated di--butylpyrene compound with NO[Al(OR)] (OR = OC(CF)) resulted in radical cation salt [Al(OR)] and diradical dication salt 2[Al(OR)], respectively. Both salts were isolated as stable crystals and investigated by single-crystal X-ray diffraction, EPR spectroscopy, SQUID measurements, and UV-vis spectroscopy. The electron spin density is delocalized on one single sulfur-doped ring in and on both sulfur-doped rings in . The diradical dication features a robust triplet ground state, representing the first example of a high-spin sulfur-hydrocarbon diradical.
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http://dx.doi.org/10.1021/jacs.0c02141DOI Listing
April 2020
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