Publications by authors named "Xinmei Liu"

66 Publications

Anxiety and depression-like behaviours are more frequent in aged male mice conceived by ART compared with natural conception.

Reproduction 2021 Oct 1. Epub 2021 Oct 1.

H Huang, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

The number of children born after assisted reproductive technology (ART) are accumulating rapidly and the healthy problems of the children are extensively concerned. This study aims to evaluate whether the ART procedures alter behaviours in male offspring. Mouse models were utilized to establish three groups of offspring conceived by natural conception (NC), in vitro fertilization and embryo transfer (IVF-ET) and frozen-thawed embryo transfer (IVF-FET), respectively. A battery of behaviour experiments for evaluating anxiety and depression levels, including the open field test (OFT), elevated plus maze (EPM) test, light/dark transition test (L/DTT), tail suspension test (TST), forced swimming test (FST) and sucrose preference test (SPT). Aged (18 months old), but not young (3 months old) male offspring, in the IVF-ET and IVF-FET groups, compared with these in the NC group, exhibited increased anxiety and depression-like behaviours. The protein expression levels of three neurotrophins in PFC or hippocampus in aged male offspring from the IVF-ET and IVF-FET groups reduced at different extent, in comparison to NC group. RNA Sequencing (RNA-Seq) was performed in the hippocampus of 18 months old offspring to further explore the gene expression profile changes in the three groups. KEGG analyses revealed the coexisted pathways, such as PI3K-Akt signalling pathway, which potentially reflected the similarity and divergence in anxiety and depression between the offspring conceived by IVF-ET and IVF-FET. Our research suggested the adverse effects of advanced age on the psychological health of children born after ART should be highlighted in future.
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http://dx.doi.org/10.1530/REP-21-0175DOI Listing
October 2021

Review of prophylactic swallowing interventions for head and neck cancer.

Int J Nurs Stud 2021 Nov 27;123:104074. Epub 2021 Aug 27.

The First Hospital of Jilin University, No.71 Xinmin street, Changchun, Jilin 130021, China. Electronic address:

Background: Head and neck cancer treatment destroys nerves and/or organs associated with swallowing. Previous studies have investigated the efficacy of exercises for muscles used in swallowing before treatment in reducing disuse atrophy and delaying the occurrence of muscle fibrosis. However, the rehabilitation effects of training and the optimal intervention strategy are unknown.

Objectives: To establish evidence for the efficacy of prophylactic swallowing interventions in reducing aspiration and restoring oral intake in patients with head and neck cancer with dysphagia.

Methods: We searched electronic databases (PubMed, Embase, Cochrane and MEDLINE) for studies published up to June 2021 reporting outcomes following prophylactic swallowing interventions in patients with head and neck cancer with dysphagia and the related influencing factors. The methodological quality of the literature was assessed using the Joanna Briggs Institute appraisal tools.

Results: The search identified 1468 articles, and 13 studies were eventually included. Four categories involving 12 different swallowing interventions were classified. Regarding the descriptive analysis of the rehabilitation effects across all studies, in terms of oropharyngeal safety, five studies showed that swallowing interventions reduced the risk of aspiration, penetration or residue. In terms of oral intake and tube feeding dependence, four studies demonstrated reduced time to return to oral intake in the intervention group compared with the control group. In terms of intervention adherence, three studies showed that speech-language pathologist- and nurse-supervised training was a potential promoter of adherence, and five studies showed that the negative factors affecting adherence included pain, fatigue, forgetting, smoking, decreased exercise motivation, side effects of radiotherapy and distance to the rehabilitation site.

Conclusions: Preventive swallowing interventions may be effective at reducing aspiration, improving swallowing function, and restoring oral intake. However, due to the lack of standardization and consistency of interventions and measurement results, which prevented the production of a best practice guide, future rigorous methodological trials will be needed to determine the most effective interventions for maximizing exercise adherence over the long term.
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http://dx.doi.org/10.1016/j.ijnurstu.2021.104074DOI Listing
November 2021

Circular RNA PUM1 (CircPUM1) attenuates trophoblast cell dysfunction and inflammation in recurrent spontaneous abortion via the MicroRNA-30a-5p (miR-30a-5p)/JUNB axis.

Bioengineered 2021 12;12(1):6878-6890

Department of Gynecology and Obstetrics, The Third Affiliated Hospital of Soochow University, Changzhou, China.

Recurrent spontaneous abortion (RSA) is a threat to human reproductive health worldwide. CircPUM1 has been reported to participate in the pathogenesis of various diseases. However, there has been no report on its association with RSA yet. In this study, gene expressions were examined by RT-qPCR. Protein levels of JUNB and cleaved caspases-3 were detected by Western blotting. ELISA was used to detect TNF-α, IL-6, and IL-8 levels. Cell viability, migration, invasion, and apoapsis were analyzed using CCK-8, transwell, and flow cytometry assays. The association between miR-30a-5p and circPUM1 or JUNB was identified by bioinformatics analysis, dual-luciferase reporter assay, and RIP assay. Herein, we found circPUM1 was significantly downregulated in RSA placental samples. CircPUM1 knockdown induced decreased proliferation, migration, and invasion, but increased apoptosis, pro-apoptotic protein (cleaved caspases-3) level, and proinflammatory factor (TNF-α, IL-6, and IL-8) secretion in trophoblast cells. Furthermore, we confirmed that circPUM1 was a sponge for miR-30a-5p, and JUNB was directly targeted by miR-30a-5p. It was demonstrated that miR-30a-5p inhibition could reverse trophoblast cell dysfunction and inflammation induced by circPUM1 knockdown. In addition, we found that JUNB expression was negatively modulated by miR-30a-5p and positively regulated by circPUM1. Moreover, circPUM1 inhibition exacerbated dysfunction and inflammation in trophoblast cells via targeting JUNB. To sum up, our study indicated that circPUM1 could impair RSA occurrence and development by facilitating trophoblast cellular processes and protecting against inflammation via the miR-30a-5p/JUNB axis, providing a new target for the improvement of RSA diagnosis and treatment.
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http://dx.doi.org/10.1080/21655979.2021.1973207DOI Listing
December 2021

Scale-up reactivation of spent S-Zorb adsorbents for gasoline desulfurization.

J Hazard Mater 2021 Aug 13;423(Pt A):126903. Epub 2021 Aug 13.

State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao 266555, China; College of Chemical Engineering, China University of Petroleum, Qingdao 266555, China.

Reactivating and recycling spent S-Zorb adsorbents reduce fresh adsorbents consumption and hazardous wastes emissions. Though the spent adsorbents have been successfully reactivated in the laboratory, a pilot-scale practice is indispensable before the industrial production. Herein, the reactivation of spent adsorbents was performed at laboratory (1.0 L), middle (10 L) and pilot (3000 L) scale, respectively. The inert ZnSiO and ZnS over the spent adsorbents are recovered to active ZnO, and the NiS is transformed into NiO. There is almost no amplification effect in pore structure and acidity of the reactivated adsorbents, while NiO particle size reduces slightly with the reactivation scales. The computational fluid dynamic simulation indicates that enhanced contact between spent adsorbents and acid/alkaline reagents at larger scale account for the smaller NiO particle. It provides more hydrogenolysis centers for CS bonds breakage after reduction, increasing initial desulfurization activity. More importantly, the adsorbent reactivated at pilot scale exhibits comparable activity to the fresh one in gasoline desulfurization. The sulfur content in the outlet decreases to less than 10 μg g from 1 h of reaction. Thus, the reactivation of spent S-Zorb adsorbents is successfully scaled up to the pilot scale, accelerating industrial practice in recycling the spent adsorbents.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126903DOI Listing
August 2021

Intrauterine hyperglycemia impairs memory across two generations.

Transl Psychiatry 2021 08 20;11(1):434. Epub 2021 Aug 20.

Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China.

Studies on humans and animals suggest associations between gestational diabetes mellitus (GDM) with increased susceptibility to develop neurological disorders in offspring. However, the molecular mechanisms underpinning the intergenerational effects remain unclear. Using a mouse model of diabetes during pregnancy, we found that intrauterine hyperglycemia exposure resulted in memory impairment in both the first filial (F1) males and the second filial (F2) males from the F1 male offspring. Transcriptome profiling of F1 and F2 hippocampi revealed that differentially expressed genes (DEGs) were enriched in neurodevelopment and synaptic plasticity. The reduced representation bisulfite sequencing (RRBS) of sperm in F1 adult males showed that the intrauterine hyperglycemia exposure caused altered methylated modification of F1 sperm, which is a potential epigenetic mechanism for the intergenerational neurocognitive effects of GDM.
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http://dx.doi.org/10.1038/s41398-021-01565-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379206PMC
August 2021

Advances in the role of silence information regulator family in pathological pregnancy.

Zhejiang Da Xue Xue Bao Yi Xue Ban 2021 Jun;50(3):335-344

Women's Hospital.

Aberrant maternal inflammation and oxidative stress are the two main mechanisms of pathological pregnancy. The silence information regulator (sirtuin) family is a highly conserved family of nicotinamide adenine dinucleotide (NAD)-dependent deacylases. By regulating the post-translational modification of proteins, sirtuin is involved in various biological processes including oxidative stress and inflammation. Nowadays, emerging evidence indicates that sirtuin may be closely related to the occurrence and development of pathological pregnancy. The down-regulation of sirtuin can cause spontaneous preterm delivery by promoting uterine contraction and rupture of fetal membranes, cause gestational diabetes mellitus through promoting oxidative stress and affecting the activity of key enzymes in glucose metabolism, cause preeclampsia by reducing the proliferation and invasion ability of trophoblasts, cause intrahepatic cholestasis of pregnancy by promoting the production of bile acids and T helper 1 cell (Th1) cytokines, and cause intrauterine growth restriction through inducing mitochondrial dysfunction. Moreover, the expression and activation of sirtuin can be modulated through dietary interventions, thus sirtuin is expected to become a new target for the prevention and treatment of pregnancy complications. This article reviews the role of the sirtuin family in the occurrence and development of pathological pregnancy and its influence on the development of the offspring.
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http://dx.doi.org/10.3724/zdxbyxb-2021-0183DOI Listing
June 2021

Comparison of Genome-Wide DNA Methylation Profiles of Human Fetal Tissues Conceived by Fertilization and Natural Conception.

Front Cell Dev Biol 2021 14;9:694769. Epub 2021 Jul 14.

International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Assisted reproductive technology (ART) might induce adverse pregnancy outcomes and increase the risk of metabolic diseases in offspring' later life with unknown reasons. Here we evaluated the global methylation level and methylation profile of fetal tissue from elective terminations of pregnancy (ETP) after natural conception and multifetal pregnancy reduction (MFPR) after fertilization and embryo transfer (IVF-ET).

Results: Global methylation levels were comparable between the fetal tissue of ETP after natural conception group and MFPR after IVF-ET group. The methylation levels were lower in the hypermethylated regions of the MFPR group than in the ETP group, while the methylation levels were higher in the hypomethylated regions of the MFPR group. Heatmap visualization and hierarchical clustering of the candidate differentially methylated regions (DMRs) showed differences between the DMRs in the ETP and MFPR samples. We identified 196 differentially methylated regions that matched 164 genes between the ETP and MFPR groups. In the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, skeletal system morphogenesis and diabetes mellitus ranked first. Ingenuity Pathway Analysis (IPA) revealed 8 diseases and functional annotations associated with IVT-ET. In the MFPR group, the final validation showed lower methylation levels in gene bodies of bone morphogenetic protein 4 (BMP4), higher methylation levels in the 1st exon and 5'UTR of thyroid peroxidase (TPO), and higher methylation levels in TSS1500 and TSS200 of interleukin 1 beta (IL1B).

Conclusions: ART does not alter global DNA methylation level, but influences DNA methylation variation in specific regions of human fetus in the early stage of life. Further studies are warranted to clarify the potential role of DNA methylation alterations in the gene expression profile.
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http://dx.doi.org/10.3389/fcell.2021.694769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318003PMC
July 2021

Enhanced dispersion of nickel nanoparticles on SAPO-5 for boosting hydroisomerization of n-hexane.

J Colloid Interface Sci 2021 Dec 10;604:727-736. Epub 2021 Jul 10.

State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao 266555, China.

The nickel based bifunctional catalyst with enhanced hydroisomerization performance was developed using an in-situ solid synthesis method. It was achieved to stabilize smaller Ni active sites on SAPO-5 using ethylenediaminetetraacetic acid (EDTA) ligands. The role of EDTA ligands was clarified by controlling the molar ratio of EDTA to Ni (EDTA/Ni) over Ni/SAPO-5 catalysts. EDTA ligands inhibited the formation of nickel aluminate spinel and aggregation of NiO species during calcination, which dispersed Ni nanoparticles in a mean size of 4.7 nm on SAPO-5. The size of Ni nanoparticles could be controlled by regulating EDTA/Ni ratio in [Ni-EDTA] complex. The prepared catalyst exhibited high yield of isomers (54.0%) and di-branched isomers selectivity (18.0%) in the n-hexane hydroisomerization, which was approximately 2 times higher than that of the Ni/SAPO-5 catalyst without EDTA ligands at similar conversion. These results are important to propose a facile approach for the preparation of highly dispersed non-noble metal based bifunctional catalysts at a high loading.
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http://dx.doi.org/10.1016/j.jcis.2021.07.039DOI Listing
December 2021

Integrative Single-Cell Transcriptomic Analysis of Human Fetal Thymocyte Development.

Front Genet 2021 2;12:679616. Epub 2021 Jul 2.

Shanghai Key Laboratory of Embryo Original Disease, Shanghai, China.

Intrathymic differentiation of T lymphocytes begins as early as intrauterine stage, yet the T cell lineage decisions of human fetal thymocytes at different gestational ages are not currently understood. Here, we performed integrative single-cell analyses of thymocytes across gestational ages. We identified conserved candidates underlying the selection of T cell receptor (TCR) lineages in different human fetal stages. The trajectory of early thymocyte commitment during fetal growth was also characterized. Comparisons with mouse data revealed conserved and species-specific transcriptional dynamics of thymocyte proliferation, apoptosis and selection. Genome-wide association study (GWAS) data associated with multiple autoimmune disorders were analyzed to characterize susceptibility genes that are highly expressed at specific stages during fetal thymocyte development. In summary, our integrative map describes previously underappreciated aspects of human thymocyte development, and provides a comprehensive reference for understanding T cell lymphopoiesis in a self-tolerant and functional adaptive immune system.
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http://dx.doi.org/10.3389/fgene.2021.679616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284395PMC
July 2021

Understanding the Fundamentals of Microporosity Upgrading in Zeolites: Increasing Diffusion and Catalytic Performances.

Adv Sci (Weinh) 2021 Sep 4;8(17):e2100001. Epub 2021 Jul 4.

State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China.

Hierarchical zeolites are regarded as promising catalysts due to their well-developed porosity, increased accessible surface area, and minimal diffusion constraints. Thus far, the focus has been on the creation of mesopores in zeolites, however, little is known about a microporosity upgrading and its effect on the diffusion and catalytic performance. Here the authors show that the "birth" of mesopore formation in faujasite (FAU) type zeolite starts by removing framework T atoms from the sodalite (SOD) cages followed by propagation throughout the crystals. This is evidenced by following the diffusion of xenon (Xe) in the mesoporous FAU zeolite prepared by unbiased leaching with NH F in comparison to the pristine FAU zeolite. A new diffusion pathway for the Xe in the mesoporous zeolite is proposed. Xenon first penetrates through the opened SOD cages and then diffuses to supercages of the mesoporous zeolite. Density functional theory (DFT) calculations indicate that Xe diffusion between SOD cage and supercage occurs only in hierarchical FAU structure with defect-contained six-member-ring separating these two types of cages. The catalytic performance of the mesoporous FAU zeolite further indicates that the upgraded microporosity facilitates the intracrystalline molecular traffic and increases the catalytic performance.
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http://dx.doi.org/10.1002/advs.202100001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425932PMC
September 2021

The Risk Factors-Based Nursing Case Management Could Effectively Reduce the Incidence of Pressure Sores in Hospitalized Patients.

Iran J Public Health 2021 Mar;50(3):566-572

Department of Pharmacy, Guangzhou Red Cross Hospital, Ji-Nan University, Guangzhou, 510220, P.R. China.

Background: To explore the effects of risk factors-based nursing management on the occurrence of pressure sores in hospitalized patients.

Methods: From Jan 2018 to Jun 2018, 289 hospitalized patients were divided into pressure sores group [100] and control group [189] for retrospective analysis. Overall, 260 hospitalized patients from Jun 2018 to Dec 2018 were followed up for nursing intervention. Overall 130 patients received risk factors-based nursing case management were in the intervention group, whereas 130 patients who received routine nursing care were in the control group. The chi-square test and -test were used to compare the count data and the measurement data between groups, respectively.

Results: Age, body weight and proportions of patients with impaired nutritional intake, diabetes or stroke in pressure sores group were higher than those in normal group (<0.05). Hospital stay and operative time in pressure sores group was longer than those in normal group (<0.05). The frequency of assistant activity in pressure sores group was significantly lower than that in control group (<0.05).In addition, the score of uroclepsia in pressure sores group was lower than that in normal group (<0.05). Patients in the intervention group showed lower risk for pressure sores and more satisfied than patients in control group (<0.001).

Conclusion: Advanced age, high body weight, diabetes and stroke, long hospital stay, long operative time, poor nutritional status and severe uroclepsia were independent risk factors of pressure sores. Risk factors-based nursing case management can effectively reduce the occurrence and risk of pressure sores for hospitalized patients.
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http://dx.doi.org/10.18502/ijph.v50i3.5601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214607PMC
March 2021

A novel smartphone-based electrochemical cell sensor for evaluating the toxicity of heavy metal ions Cd, Hg, and Pb in rice.

Anal Bioanal Chem 2021 Jul 31;413(16):4277-4287. Epub 2021 May 31.

College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, 210023, Jiangsu, China.

A novel smartphone-based electrochemical cell sensor was developed to evaluate the toxicity of heavy metal ions, such as cadmium (Cd), lead (Pb), and mercury (Hg) ions on Hep G2 cells. The cell sensor was fabricated with reduced graphene oxide (RGO)/molybdenum sulfide (MoS) composites to greatly improve the biological adaptability and amplify the electrochemical signals. Differential pulse voltammetry (DPV) was employed to measure the electrical signals induced by the toxicity of heavy metal ions. The results showed that Cd, Hg, and Pb significantly reduced the viability of Hep G2 cells in a dose-dependent manner. The IC values obtained by this method were 49.83, 36.94, and 733.90 μM, respectively. A synergistic effect was observed between Cd and Pb and between Hg and Pb, and an antagonistic effect was observed between Cd and Hg, and an antagonistic effect at low doses and an additive effect at high doses were found in the ternary mixtures of Cd, Hg, and Pb. These electrochemical results were confirmed via MTT assay, SEM and TEM observation, and flow cytometry. Therefore, this new electrochemical cell sensor provided a more convenient, sensitive, and flexible toxicity assessment strategy than traditional cytotoxicity assessment methods.
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http://dx.doi.org/10.1007/s00216-021-03379-4DOI Listing
July 2021

Early-life undernutrition induces enhancer RNA remodeling in mice liver.

Epigenetics Chromatin 2021 Mar 31;14(1):18. Epub 2021 Mar 31.

The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Maternal protein restriction diet (PRD) increases the risk of metabolic dysfunction in adulthood, the mechanisms during the early life of offspring are still poorly understood. Apart from genetic factors, epigenetic mechanisms are crucial to offer phenotypic plasticity in response to environmental situations and transmission. Enhancer-associated noncoding RNAs (eRNAs) transcription serves as a robust indicator of enhancer activation, and have potential roles in mediating enhancer functions and gene transcription.

Results: Using global run-on sequencing (GRO-seq) of nascent RNA including eRNA and total RNA sequencing data, we show that early-life undernutrition causes remodeling of enhancer activity in mouse liver. Differentially expressed nascent active genes were enriched in metabolic pathways. Besides, our work detected a large number of high confidence enhancers based on eRNA transcription at the ages of 4 weeks and 7 weeks, respectively. Importantly, except for ~ 1000 remodeling enhancers, the early-life undernutrition induced instability of enhancer activity which decreased in 4 weeks and increased in adulthood. eRNA transcription mainly contributes to the regulation of some important metabolic enzymes, suggesting a link between metabolic dysfunction and enhancer transcriptional control. We discovered a novel eRNA that is positively correlated to the expression of circadian gene Cry1 with increased binding of epigenetic cofactor p300.

Conclusions: Our study reveals novel insights into mechanisms of metabolic dysfunction. Enhancer activity in early life acts on metabolism-associated genes, leading to the increased susceptibility of metabolic disorders.
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http://dx.doi.org/10.1186/s13072-021-00392-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011416PMC
March 2021

Abnormal Glucose Metabolism in Male Mice Offspring Conceived by Fertilization and Frozen-Thawed Embryo Transfer.

Front Cell Dev Biol 2021 9;9:637781. Epub 2021 Feb 9.

The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Frozen and thawed embryo transfer (FET) is currently widely applied in routine assisted reproductive technology (ART) procedure. It is of great necessity to assess the safety of FET and investigate the long-term effect including glucose metabolism on FET-conceived offspring. The mouse model is a highly efficient method to figure out the relationship between the process of FET and offspring health. In this study, we obtained mouse offspring of natural conception (NC), fertilization (IVF), and FET. Glucose and insulin tolerance test (GTT/ITT) were performed on both chow fed or high fat diet (HFD) fed offspring to examine the glucose metabolism status. We detected hepatic PI3K/AKT pathway by western blotting and transcriptome status by RNA-sequencing. Impaired glucose tolerance (IGT) and decreased insulin tolerance were occurred in FET conceived male offspring. After challenged with the HFD-fed, male offspring in FET group performed earlier and severer IGT than IVF group. Furthermore, higher HOMA-IR index and higher serum insulin level post glucose injected in FET-chow group suggested the insulin resistance status. The PI3K/AKT signaling pathway, the major pathway of insulin in the liver, were also disrupted in FET group. Transcriptomics of the liver reveals significantly downregulated in glucose metabolic process and insulin resistance in the FET-chow group. In our study, FET-conceived male mouse offspring presented glucose metabolism dysfunction mainly manifesting insulin resistance. The hepatic insulin signaling pathway were in concordance with reduced glycogen synthesis, increased glycolysis and enhanced gluconeogenesis status in FET-conceived male offspring.
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http://dx.doi.org/10.3389/fcell.2021.637781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900417PMC
February 2021

Corrigendum: Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy Women.

Front Physiol 2020 18;11:635088. Epub 2021 Jan 18.

Department of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, China.

[This corrects the article DOI: 10.3389/fphys.2020.01108.].
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http://dx.doi.org/10.3389/fphys.2020.635088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848215PMC
January 2021

Silicon stent placement via rigid bronchoscopy for the treatment of central airway obstruction in infants: Case series.

Medicine (Baltimore) 2021 Jan;100(2):e24244

Department of Otolaryngology, Head and Neck Surgery, the First Hospital of Jilin University, Changchun, China.

Introduction: Rigid bronchoscopy has been proven to be an excellent tool for the diagnosis and management of several causes of central airway obstruction (CAO). The invasive treatment of silicone bronchobrachial stenting has been performed in children and adults with CAO, and satisfying results were obtained in previous studies. However, there are few reports on infants with central airway obstruction treated with stenting via rigid bronchoscopy. This technique remains a challenge to pediatric thoracic surgeons, pediatric interventional pulmonologists, and otolaryngologists who struggle to treat airway obstruction disease.

Patient Concerns: Four patients were presented to our hospital with complaints of dyspnea for a period of time after their birth.

Diagnosis: Three patients were diagnosed as tracheobronchomalacia, and tracheoesophageal fistula.

Interventions: Four patients were treated with silicone stenting through rigid bronchoscopy.

Outcomes: Silicon stent was adequate for improving the obstruction of the tracheal tract. All the patients were followed-up longer than 6 months. Three patients could breathe normally; the stent migrated in only 1 patient.

Conclusion: Invasive silicone tracheobronchial stenting via rigid bronchoscopy is a viable option for infants with CAO. Choosing an appropriate size is a critical factor for success of stenting according to our experience.
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http://dx.doi.org/10.1097/MD.0000000000024244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808444PMC
January 2021

Steroid metabolome profiling of follicular fluid in normo- and hyperandrogenic women with polycystic ovary syndrome.

J Steroid Biochem Mol Biol 2021 02 16;206:105806. Epub 2020 Dec 16.

The International Peace Maternal and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China. Electronic address:

Polycystic ovary syndrome (PCOS) is a heterogeneous disease defined by the presence of at least two of the following features: hyperandrogenism, oligoanovulation (OA), and polycystic ovarian morphology (PCOM). Hyperandrogenism is considered the cornerstone of PCOS. However, the most prevalent phenotype in Chinese women with PCOS is OA + PCOM [normo-androgenic PCOS (NA-PCOS)]. It has been reported that PCOS women have higher androgen levels in follicular fluid (FF), but whether NA-PCOS women have the same intrafollicular steroid profiles as hyperandrogenic PCOS (HA-PCOS) women has not been explored. In this study, we analyzed 17 steroids in stimulated size-matched ovarian follicles (16-18 mm) from 166 controls and 141 PCOS women [87 NA-PCOS and 54 HA-PCOS women, defined by a single serum testosterone (T) immunoassay measurement] using liquid chromatography tandem mass spectrometry, and investigated their relationship with baseline characteristics. No significant differences in intrafollicular steroid levels and product/precursor ratios between NA-PCOS and HA-PCOS women were observed, though HA-PCOS women had significantly higher serum luteinizing hormone and T levels than NA-PCOS women. NA-PCOS and HA-PCOS women had significantly higher levels of androstenedione (AD), T and free androgen index, higher enzyme activity of P450c17 (AD/17OH-progesterone), 3βHSD2 (17OH-progesterone /17OH-pregnenolone) and P450c11 (corticosterone /11-deoxycorticosterone), lower levels of pregnenolone, 17OH-pregnenolone and 11-deoxycorticosterone, and decreased enzyme activity of P450aro (estrone/AD and estradiol/T) and 5α-reductase (dihydrotestosterone/T) in FF than controls. NA-PCOS women had significantly higher intrafollicular cortisol levels and lower 11βHSD2 (cortisone/cortisol) activity than controls. Baseline serum T levels were slightly correlated with intrafollicular estrogens (E1: r = 0.192, p = 0.019; E2: r = 0.248, p = 0.002; E3: r = 0.248, p = 0.002) and androgens (DHEAS: r = 0.276, p = 0.001; AD: r = 0.185, p = 0.032; T: r = 0.173, p = 0.044) in controls and PCOS women respectively. Serum anti-Müllerian hormone (AMH) levels and antral follicle count (AFC) were correlated with intrafollicular cortisol (AMH: r = 0.380, p = 0.000; AFC: r = 0.177, p = 0.036) and corticosterone (AMH: r = 0.212, p = 0.048; AFC: r = 0.219, p = 0.009) levels in PCOS women. In conclusion, NA-PCOS and HA-PCOS women had statistically similar steroid metabolome profiles in FF, both of which showed a generally decreased steroidogenesis and hyperandrogenism compared to controls.
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http://dx.doi.org/10.1016/j.jsbmb.2020.105806DOI Listing
February 2021

AQP7 mediates post-menopausal lipogenesis in adipocytes through FSH-induced transcriptional crosstalk with AP-1 sites.

Reprod Biomed Online 2020 Dec 18;41(6):1122-1132. Epub 2020 Aug 18.

International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China; Shanghai Key Laboratory of Embryo Original Disease Shanghai, China; Key Laboratory of Reproductive Genetics (Zhejiang University), Ministry of Education, Hangzhou Zhejiang, China. Electronic address:

Research Question: Fat accumulation is present in most post-menopausal women, but the underlying mechanism remains unclear. Aquaporin 7 (AQP7) is the most important glycerol channel facilitating glycerol efflux in adipocytes. High circulating FSH in post-menopausal women may play an independent role in regulation of the lipogenic effect of AQP7 in adipocytes. This study explored the role of AQP7 in the pathophysiology of post-menopausal lipogenesis mediated by high concentrations of circulating FSH.

Design: Primary adipocytes from post-menopausal and childbearing women were analysed. An in-vivo post-menopausal animal model was established. AQP7 expression, lipid accumulation and glycerol concentration in adipocytes were measured. Luciferase reporter assay and chromatin immunoprecipitation were performed to identify transcriptional crosstalk in AQP7 promoter.

Results: It was found that FSH down-regulated AQP7 expression and glycerol efflux function in mature adipocytes of post-menopausal women and ovariectomized (OVX) mice. In vitro, FSH inhibited lipid accumulation in primary cultured mature adipocytes in a dose-dependent manner and the mechanism was down-regulating AQP7 expression via a FSH receptor pathway. The effect of FSH on AQP7 in adipocytes was through activation of cAMP response element-binding (CREB) protein, which could bind to activator protein-1 (AP-1) sites in the AQP7 promoter, and therefore inhibited the transcriptional activation elicited by c-Jun.

Conclusions: Down-regulation of AQP7 by FSH mediated post-menopausal lipogenesis, and the role of FSH was based on binding competition for AP-1 sites between CREB and c-Jun.
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http://dx.doi.org/10.1016/j.rbmo.2020.08.008DOI Listing
December 2020

Novel PGK1 determines SKP2-dependent AR stability and reprograms granular cell glucose metabolism facilitating ovulation dysfunction.

EBioMedicine 2020 Nov 21;61:103058. Epub 2020 Oct 21.

International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China; Institute of Embryo-Fetal Original Adult Diseases Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Background: Disordered folliculogenesis is a core characteristic of polycystic ovary syndrome (PCOS) and androgen receptors (ARs) are closely associated with hyperandrogenism and abnormalities in folliculogenesis in PCOS. However, whether the new AR binding partner phosphoglycerate kinase 1 (PGK1) in granulosa cells (GCs) plays a key role in the pathogenesis of PCOS remains unclear.

Methods: We identified the new AR binding partner PGK1 by co-IP (co-immunoprecipitation) in luteinized GCs, and reconfirmed by co-IP, co-localization and GST pull down assay, and checked PGK1 expression levels with qRT-PCR and western blotting. Pharmaceuticals rescue assays in mice, and metabolism assay, AR protein stability and RNA-seq of PGK1 targets in cells proved the function in PCOS.

Findings: PGK1 and AR are highly expressed in PCOS luteinized GCs and PCOS-like mouse ovarian tissues. PGK1 regulated glucose metabolism and deteriorated PCOS-like mouse metabolic disorder, and paclitaxel rescued the phenotype of PCOS-like mice and reduced ovarian PGK1 and AR protein levels. PGK1 inhibited AR ubiquitination levels and increased AR stability in an E3 ligase SKP2-dependent manner. Additionally, PGK1 promoted AR nuclear translocation, and RNA-seq data showed that critical ovulation-related genes were regulated by the PGK1-AR axis.

Interpretation: PGK1 regulated GCs metabolism and interacted with AR to regulate the expression of key ovulation genes, and also mediated cell proliferation and apoptosis, which resulted in the etiology of PCOS. This work highlights the pathogenic mechanism and represents a novel therapeutic target for PCOS.

Funding: National Key Research and Development Program of China; National Natural Science Foundation of China grant.
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http://dx.doi.org/10.1016/j.ebiom.2020.103058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581881PMC
November 2020

Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy Women.

Front Physiol 2020 8;11:1108. Epub 2020 Sep 8.

Department of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, China.

Women with polycystic ovarian syndrome (PCOS) often have a history of infertility and poor pregnancy outcome. The character of the lower genital tract (LGT) microbiome of these patients is still unknown. We collected both vaginal and cervical canal swabs from 47 PCOS patients (diagnosed by the Rotterdam Criteria) and 50 healthy reproductive-aged controls in this study. Variable regions 3-4 (V3-4) were sequenced and analyzed. Operational taxonomic unit (OTU) abundance was noted for all samples. Taxa that discriminated between PCOS and healthy women was calculated by linear discriminant analysis effect size (LEFSe). Results from 97 paired vaginal and cervical canal samples collected from 97 women [mean age 30 (±4 years)] were available for analysis. Using the Rotterdam Criteria, 47 women were diagnosed with PCOS (PCOS, = 47; control, = 50). There was no significant difference between cervical canal microbiome and vaginal microbiome from the same individual, however, spp. was less abundant in both vaginal and cervical canal microbiome of PCOS patients. Several non- taxa including , , and , were more abundant in the LGT microbiota of PCOS patients. There is a difference between the microorganism in the LGT of patients with PCOS and healthy reproductive-aged women.
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http://dx.doi.org/10.3389/fphys.2020.01108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506141PMC
September 2020

Long noncoding RNA CBR3 antisense RNA 1 promotes the aggressive phenotypes of non‑small‑cell lung cancer by sponging microRNA‑509‑3p and competitively upregulating HDAC9 expression.

Oncol Rep 2020 10 7;44(4):1403-1414. Epub 2020 Aug 7.

Department of Respiratory Medicine, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.

Long noncoding RNA CBR3 antisense RNA 1 (CBR3‑AS1) plays significant roles in the initiation and progression of osteosarcoma. The aim of the present study was to investigate the involvement of CBR3‑AS1 in the development of non‑small cell lung cancer (NSCLC). Reverse transcription‑quantitative PCR was performed to detect CBR3‑AS1 expression in NSCLC tissues and cell lines. The impacts of CBR3‑AS1 on cellular proliferation, apoptosis, migration and invasiveness in vitro, and tumor growth in vivo, were investigated using the Cell Counting Kit‑8 assay, flow cytometry, Transwell migration and invasion assays, and tumor xenograft model‑based analysis, respectively. The results indicated that CBR3‑AS1 was markedly upregulated in NSCLC tissues and cell lines. High CBR3‑AS1 expression was correlated with larger tumor size, advanced TNM stage, increased incidence of lymph node metastasis and shorter overall survival times in patients with NSCLC. Furthermore, CBR3‑AS1‑knockdown notably suppressed cellular proliferation, migration and invasiveness in vitro, and also promoted apoptosis and suppressed tumorigenicity in vivo. Mechanistic investigation demonstrated that CBR3‑AS1 functions as a competing endogenous RNA for microRNA‑509‑3p (miR‑509‑3p) in NSCLC cells. Furthermore, miR‑509‑3p exerted tumor‑suppressive effects in NSCLC, and histone deacetylase 9 (HDAC9) was identified as a direct target of miR‑509‑3p. HDAC9 expression was suppressed by CBR3‑AS1 depletion, which was abolished by miR‑509‑3p inhibition. Further rescue experiments revealed that increasing the output of the miR‑509‑3p/HDAC9 axis counteracted the CBR3‑AS1 depletion‑induced inhibitory effects on NSCLC cells. Collectively, the results of the present study indicate that the CBR3‑AS1/miR‑509‑3p/HDAC9 pathway exerts tumor‑promoting actions in NSCLC oncogenesis and progression, suggesting that this pathway is an effective target for the management of NSCLC.
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http://dx.doi.org/10.3892/or.2020.7719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448412PMC
October 2020

Aging attenuates the ovarian circadian rhythm.

J Assist Reprod Genet 2021 Jan 14;38(1):33-40. Epub 2020 Sep 14.

The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, 910 Hengshan Road, Shanghai, 200030, China.

Objective: To study the effect of aging on ovarian circadian rhythm.

Design: Human and animal study.

Setting: University hospital and research laboratory.

Patients/animals: Human granulosa cells were obtained by follicular aspiration from women undergoing in vitro fertilization (IVF), and ovarian and liver tissues were obtained from female C57BL/6 mice.

Intervention(s): None.

Main Outcome Measure(s): Expression of circadian genes in young and older human granulosa cells and circadian rhythm in ovaries and livers of young and older mice.

Result(s): All examined circadian clock genes in human granulosa cells showed a downward trend in expression with aging, and their mRNA expression levels were negatively correlated with age (P < 0.05). Older patients (≥ 40 years of age) had significantly reduced serum anti-Müllerian hormone (AMH) levels. Except for Rev-erbα, all other examined circadian clock genes were positively correlated with the level of AMH (P < 0.05). The circadian rhythm in the ovaries of older mice (8 months) was changed significantly relative to that in ovaries of young mice (12 weeks), although the circadian rhythm in the livers of older mice was basically consistent with that of young mice.

Conclusion(s): Lower ovarian reserve in older women is partially due to ovarian circadian dysrhythmia as a result of aging.
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http://dx.doi.org/10.1007/s10815-020-01943-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822988PMC
January 2021

Distinct mRNA and long non-coding RNA expression profiles of decidual natural killer cells in patients with early missed abortion.

FASEB J 2020 11 11;34(11):14264-14286. Epub 2020 Sep 11.

International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Early non-chromosome-related missed abortion (MA) is commonly associated with an altered immunological environment during pregnancy. Human decidual natural killer (dNK) cells, the most abundant lymphocyte population within the first-trimester maternal-fetal interface, are vital maternal regulators of immune tolerance mediating successful embryo implantation and placentation. Previous studies have shown that dNK cells may play a role in MA. However, the gene expression status and specific altered manifestations of dNK cells in patients with early MA remain largely unknown. Here, we show that MA dNK cells have distinct mRNA and lncRNA expression profiles through RNA sequencing, with a total of 276 mRNAs and 67 lncRNAs being differentially expressed compared with controls. Protein-protein interaction analysis of differentially expressed mRNAs was performed to identify hub genes and key modules. An lncRNA-mRNA regulatory network characterized by the small-world property was constructed to reveal the regulation of mRNA transcription by differential hub lncRNAs. Functional annotation of differentially expressed mRNAs and lncRNAs was performed to disclose their potential roles in MA pathogenesis. Our data highlight several enriched biological processes (immune response, inflammatory response, cell adhesion, and extracellular matrix [ECM] organization) and signaling pathways (cytokine-cytokine receptor interaction, ECM-receptor interaction, Toll-like receptor signaling pathway, and phosphatidylinositol signaling system) that may influence MA. This study is the first to demonstrate the involvement of altered mRNA and lncRNA expression profiles in the dNK cell pathogenesis of early MA, facilitating a better understanding of the underlying molecular mechanisms and the development of novel MA therapeutic strategies targeting key mRNAs and lncRNAs.
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http://dx.doi.org/10.1096/fj.202000621RDOI Listing
November 2020

Long noncoding RNA OSER1‑AS1 promotes the malignant properties of non‑small cell lung cancer by sponging microRNA‑433‑3p and thereby increasing Smad2 expression.

Oncol Rep 2020 08 12;44(2):599-610. Epub 2020 Jun 12.

Department of Respiratory Disease, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.

OSER1 antisense RNA 1 (OSER1‑AS1), a long noncoding RNA, has been well studied in the context of hepatocellular carcinoma. However, its expression status, specific functions, and tumorigenic mechanism in non‑small cell lung cancer (NSCLC) remain uninvestigated. Hence, this study aimed to assess OSER1‑AS1 expression, test the malignancy‑related biological functions of OSER1‑AS1, and illustrate how they affect NSCLC progression. OSER1‑AS1 expression in NSCLC was measured by reverse transcription‑quantitative polymerase chain reaction. Cell Counting Kit‑8 assay, flow cytometry, cell migration and invasion assay, and tumor xenograft assay were performed to analyze the effects of OSER1‑AS1 on the malignant phenotypes of NSCLC cells. Bioinformatics prediction with luciferase reporter and RNA immunoprecipitation assays were performed to determine the interaction between OSER1‑AS1 and microRNA‑433‑3p (miR‑433‑3p). OSER1‑AS1 was strongly expressed in NSCLC tissues and cell lines. Enhanced OSER1‑AS1 expression was significantly correlated with tumor size, TNM stage, and lymph node metastasis in patients with NSCLC. Patients with NSCLC exhibiting high OSER1‑AS1 expression had shorter overall survival than those exhibiting low OSER1‑AS1 expression. Functionally, a reduction in OSER1‑AS1 expression led to significant decreases in NSCLC cell proliferation, migration, and invasion as well as an increase in cell apoptosis in vivo. OSER1‑AS1 knockdown suppressed the tumorigenic ability of NSCLC cells in vivo. Mechanistically, OSER1‑AS1 acts as a competing endogenous RNA (ceRNA) in NSCLC cells by sponging miR‑433‑3p and thereby increasing the expression of mothers against decapentaplegic homolog 2 (Smad2). Finally, restoration experiments revealed that the suppression of miR‑433‑3p and restoration of Smad2 both counteracted the suppressive effects of OSER1‑AS1 depletion in NSCLC cells. Our findings illustrate the biological importance of the OSER1‑AS1/miR‑433‑3p/Smad2 pathway in NSCLC progression and offer a novel perspective regarding the identification of effective therapeutic and diagnostic targets.
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http://dx.doi.org/10.3892/or.2020.7645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336450PMC
August 2020

Giant fibroma of breast in an adolescent female by inverted "T" incision: A case report and literature review.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2020 Feb;45(2):204-207

Department of Breast and Thyroid Surgery, Central South University Xiangya School of Mediccine Affiliated Haikou Hospital, Haikou 570000, China.

Giant fibroma of the breast in adolescence is a benign tumor of the breast that occurs in a special period in women, often in adolescent women aged 18-25 years old. These tumors are characterized by short course and large size. They are rare in clinic and easy to be misdiagnosed. We report a case of 22 cm-pubertal breast giant fibroadenoma which underwent inverted "T" incision resection. The diagnosis, pathological characteristics, and treatment of adolescent giant fibroadenoma of the breast are also discussed based on literature.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2020.180529DOI Listing
February 2020

Coordination of Nanoconjugation with an Antigen/Antibody for Efficient Detection of Gynecological Tumors.

J Anal Methods Chem 2020 30;2020:6528572. Epub 2020 Mar 30.

Department of Gynecology, Xi'an Maternal and Child Health Hospital, Xi'an, Shaanxi 710002, China.

Cervical, ovarian, and endometrial cancers are common in the female reproductive system. Cervical cancer starts from the cervix, while ovarian cancer develops when abnormal cells grow in the ovary. Endometrial or uterine cancer starts from the lining of the womb in the endometrium. Approximately 12,000 women are affected every year by cervical cancer in the United States. Squamous cell carcinoma antigen (SCC-Ag) is a well-established biomarker in serum for diagnosing gynecological cancers, and its levels were observed to be elevated in cervical, ovarian, and endometrial cancer patients. Moreover, SCC-Ag was used to identify the tumor size and progression stages. Various biosensing systems have been proposed to identify SCC-Ag; herein, enhanced interdigitated electrode sensing is presented with the use of gold nanoparticles (GNPs) to conjugate an antigen/antibody. It was proved that the limit of detection is 62.5 fM in the case of antibody-GNP, which is 2-fold higher than that by SCC-Ag-GNP. Furthermore, the antibody-GNP-modified surface displays greater current increases with concomitant dose-dependent SCC-Ag levels. High analytical performance was shown by the discrimination against -fetoprotein and CYFRA 21-1 at 1 pM. An enhanced sensing system is established for gynecological tumors, representing an advance from the earlier detection methods.
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http://dx.doi.org/10.1155/2020/6528572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149363PMC
March 2020

Quality Control of L. Based on High-Speed Countercurrent Chromatographic Fingerprinting.

Molecules 2020 Jan 9;25(2). Epub 2020 Jan 9.

Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China.

Traditional Chinese medicine (TCM)has played an important role in promoting the health of Chinese people. The TCM L. has been used in the treatment of various kinds of diseases including enuresis, vitiligo, and calvities. However, therapeutic effects of L. have often influenced by the quality of plants. So, it is very important to control the quality of L. In this study, analytical high-speed countercurrent chromatography (HSCCC) was successfully used to fingerprint L. Samples of L. were extracted by ultrasonic extraction. -hexane-ethyl acetate-methanol-water at a ratio of 5:5.5:6.5:5 (/) was selected as a two-phase solvent system and the condition of HSCCC were optimized in order to good separation. And the method of HSCCC was verified (reproducibility, precision, and stability). HSCCC chromatograms exhibited six common peaks, which were selected as indicator compounds for the quality control of L. Within 20 types of medicinal materials, chemical components are similar, but the levels of components are quite different in HSCCC fingerprint. The present results demonstrate that the HSCCC method provides a reliable basis for the quality control of L. and can also be applied to confirm the authenticity of plant materials.
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http://dx.doi.org/10.3390/molecules25020279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024294PMC
January 2020

Fingerprint Analysis of Cnidium monnieri (L.) Cusson by High-Speed Counter-Current Chromatography.

Molecules 2019 Dec 8;24(24). Epub 2019 Dec 8.

Co-first authors: These authors contributed equally to this work and should be considered co-first authors..

Cnidium monnieri (L.) Cusson is a popular Traditional Chinese Medicine (TCM) with a variety of bioactivities. However, there are some problems that have affected the development of Cnidium monnieri (L.) Cusson. At present, many methods have been reported for the analysis of coumarins in Cnidium monnieri (L.) Cusson. However, the quality control of coumarins in Cnidium monnieri (L.) Cusson by high-speed counter-current chromatography (HSCCC) has not been reported. In this study, analytical high-speed counter-current chromatography (HSCCC) was successfully used for fingerprint of Cnidium monnieri (L.) Cusson with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at 4:6:6.5:3.5 (v/v). The UV wavelength was set at 254 nm. Six coumarin compounds with high biological activity were selected as indicator compounds for the quality control. The HSCCC fingerprint of the Cnidium monnieri (L.) Cusson was successfully established and there were some differences according to the results of the fingerprint analysis. The present results demonstrate that HSCCC is an established and efficient technique for the fingerprint analysis of Cnidium monnieri (L.) Cusson and can be used to control the quality of Cnidium monnieri (L.) Cusson. In brief, HSCCC is a useful technology for the fingerprint analytical method for TCM.
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http://dx.doi.org/10.3390/molecules24244496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969901PMC
December 2019

Efficacy and safety of combined nonpharmacological interventions for repeated procedural pain in preterm neonates: A systematic review of randomized controlled trials.

Int J Nurs Stud 2020 Feb 7;102:103471. Epub 2019 Nov 7.

School of Nursing, Jilin University, No. 965 Xinjiang Street, Changchun, Jilin Province 130021, China. Electronic address:

Introduction: Premature infants undergo numerous painful procedures during hospitalization. Some trials have examined the effectiveness and safety of combined nonpharmacological interventions in which two or more non-pharmacological interventions are used simultaneously or continuously to relieve repeated procedural pain via multisensory stimulation in preterm neonates. However, a systematic review of this topic has not yet been carried out.

Objective: To review the evidence on the efficacy and safety of combined nonpharmacological interventions for repeated procedural pain in preterm neonates.

Method: Eight databases were searched using keywords to identify peer-reviewed journal articles in English or Chinese. Randomized controlled trials (RCTs) focusing on combined nonpharmacological interventions for repeated procedural pain in preterm neonates published from database inception until May 2019 were included.

Results: Eight RCTs were retrieved that included ten different combined nonpharmacological interventions. Different study designs were used in the included trials, which did not allow us to carry out a meta-analysis. The findings from the included articles were categorized in terms of efficacy and safety. With respect to efficacy, 1. two trials reported that combined nonpharmacological interventions were more effective than usual care during painful procedures; 2. three trials reported that combined nonpharmacological interventions were more effective than single nonpharmacological interventions; 3. three trials reported the effects of different combinations of nonpharmacological interventions and found that their effect depends on intervention type rather than number of interventions. Only four trials reported on safety, and they found that combined nonpharmacological interventions were safe for repeated procedural pain in preterm neonates.

Conclusion: According to the literature, combined nonpharmacological interventions may be effective and safe for repeated procedural pain in premature infants. However, due to the diversity of interventions included in this systematic review, the evidence is not strong enough to produce a best practice guideline. Further research is needed with larger sample sizes and less heterogeneity to adequately explore the efficacy and safety of combined nonpharmacological interventions for repeated procedural pain in premature infants.
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http://dx.doi.org/10.1016/j.ijnurstu.2019.103471DOI Listing
February 2020

Carboxylated graphene oxide-chitosan spheres immobilize Cu in soil and reduce its bioaccumulation in wheat plants.

Environ Int 2019 12 31;133(Pt B):105208. Epub 2019 Oct 31.

College of Chemistry and Environment Protection Engineering, Southwest Minzu University, Chengdu 610041, PR China. Electronic address:

Due to the strong interaction with pollutants and the huge adsorption capacity, graphene adsorbents are widely applied in water decontamination. However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components. In this study, we prepared carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu from water and soil. The pores in GO-COOH/CS allowed the internal diffusion of Cu solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets. Therefore, the high adsorption capacity of GO-COOH/CS spheres (78 mg/g) was largely retained for the soil Cu fixation. The partition coefficient (PC) for Cu adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at C of 0.48 mg/L and q of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at C of 91.4 mg/L and q of 78 mg/g. At initial Cu concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu concentrations in leachates were lower than 1.0 mg/L. The Cu fixation on GO-COOH/CS spheres largely reduced its bioaccumulation in wheat roots from 127.8 μg/g to 51.2 μg/g. The toxicity evaluations suggested that GO-COOH/CS spheres were of low toxicity to wheat seedlings and did not amplify the toxicity of Cu. The implications to the design of graphene adsorbents for soil remediation are discussed. Overall, our results collectively indicated that porous GO-COOH/CS spheres were high-performance adsorbents for the immobilization of Cu to reduce Cu bioaccumulation in plants.
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http://dx.doi.org/10.1016/j.envint.2019.105208DOI Listing
December 2019
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