Publications by authors named "Xinhua Yin"

75 Publications

Identifying mineral decrement with bone injury by quantifying osteocalcin on current-volt sensor.

Biotechnol Appl Biochem 2021 Oct 8. Epub 2021 Oct 8.

Department of Spine Surgery, HongHui Hospital, Xi'an Jiaotong University College of Medicine, No.76 Nanguo Road, Beilin District, Xi'an, Shaanxi, 710054, China.

Osteoporosis, a bone disease is caused by the deterioration of bone and shows an enhanced risk of bone fracture and decreasing bone mineral density. Unfortunately, the available radiological techniques are expensive, and having disadvantages such as radiation intake, need a specialist to handle the instrument. This research is focused to develop a point-of-care system to identify osteocalcin on current-volt sensor, which helps to diagnose the bone metabolism and prognostics. Anti-osteocalcin antibody was attached on the electrode through the silane-modified iron material. The antibody immobilized sensing surface was utilized to identify the level of osteocalcin and the detection limit of 100 pg/mL reached on linear concentrations of 0.01-3000 ng/mL. Calculations were made by triplicates (n = 3; 3δ) on the determination coefficient of, y = 0.2637x - 0.6012; R = 0.9319. Further, control proteins failed to bind with immobilized antibody, confirmed the specific osteocalcin detection. This research is to identify the osteoporosis biomarker and helps to determine the conditions with osteoporosis. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/bab.2267DOI Listing
October 2021

Survey on sodium and potassium intake in patients with hypertension in China.

J Clin Hypertens (Greenwich) 2021 Sep 25. Epub 2021 Sep 25.

Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Sodium and potassium intake in hypertensive patients in China is not clear. The authors aimed to investigate the distribution of sodium and potassium intake in hypertensive patients in China, and to analyze the relationship between sodium and potassium intake and blood pressure. The study was performed in 130 hospitals from 23 provinces across China from 2016 to 2019. Finally, 9501 hypertensive patients average aged 54 years were included. 24 h urinary sodium and potassium excretion were measured. Distribution of urinary electrolytes were described according to age, gender and region. The association between urinary electrolytes and blood pressure was analyzed by multivariate linear regression. Hypertensive patients exhibited an average 24 h urinary sodium and potassium excretion of 156.7 ± 81.5 mmol/d and 39.2 ± 20.2 mmol/d (equivalent to sodium chloride of 9.2 g/d, potassium chloride of 2.9 g/d), sodium/potassium ratio (median) of 4.14 (2.92,5.73). Urinary electrolytes were lower in women than men (sodium: 171.1 vs 138.7, p < .05; potassium: 40.3 vs 37.7, p < .05), in the elderly than in the younger (sodium: 168.7 vs 139.9, p < .05; potassium: 39.5 vs. 37.5, p < .05). For every 1 unit of Na/K ratio increase, blood pressure increased by 0.46/0.24 mmHg. Blood pressure was 2.75/1.27 mmHg higher in quartile 4 than quartile 1 of Na/K. It remains high sodium and low potassium for hypertensive patients in China. Decreased sodium, Na/K ratio and increased potassium may help for blood pressure management.
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http://dx.doi.org/10.1111/jch.14355DOI Listing
September 2021

Trial of Intensive Blood-Pressure Control in Older Patients with Hypertension.

N Engl J Med 2021 09 30;385(14):1268-1279. Epub 2021 Aug 30.

From the Hypertension Center, FuWai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Peking Union Medical College, Chinese Academy of Medical Sciences (W.Z., S.Z., Y.D., J.C.), Peking Union Medical College Hospital (S.Z.), Beijing Pinggu Hospital (Y.L.), and Beijing Hospital (W.L.), Beijing, Kailuan General Hospital, Tangshan (S.W.), Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences (J.R.), and the First Affiliated Hospital of Shanxi Medical University (X.S.), Taiyuan, the Second Affiliated Hospital of Baotou Medical College, Baotou (G.S.), the People's Hospital of Ji Xian District, Tianjin (J.Y.), the First Affiliated Hospital of Dalian Medical University (Y.J.) and Omron Dalian (X.G.), Dalian, the First Affiliated Hospital of Xinjiang Medical University, Urumqi (X.X.), the Second Affiliated Hospital of Medical College Shantou University, Shantou (Y.C.), Benxi Railway Hospital, Benxi (L.Y.), Hongxinglong Center Hospital, Shuangyashan (D.L.), the First Affiliated Hospital of Hebei North University, Zhangjiakou (L.W.), the First Affiliated Hospital of Harbin Medical University, Harbin (X.Y.), Renmin Hospital of Wuhan University, Wuhan (X.Z.), Kang Ya Hospital, Yiyang (B.Z.), FuWai Yunnan Cardiovascular Hospital, Kunming (Z.G.), Zhoukou City Central Hospital, Zhoukou (H.L.), West China Hospital, Sichuan University, Chengdu (X.C.), Guangdong Cardiovascular Institute, Guangzhou (Y.F.), and the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an (G.T.) - all in China; the Cardiovascular Center and Divisions of Cardiology and Hospital Medicine, Department of Internal Medicine, National Taiwan University Hospital, Taipei (T.-D.W.); and Jichi Medical University School of Medicine, Shimotsuke, Japan (K.K.).

Background: The appropriate target for systolic blood pressure to reduce cardiovascular risk in older patients with hypertension remains unclear.

Methods: In this multicenter, randomized, controlled trial, we assigned Chinese patients 60 to 80 years of age with hypertension to a systolic blood-pressure target of 110 to less than 130 mm Hg (intensive treatment) or a target of 130 to less than 150 mm Hg (standard treatment). The primary outcome was a composite of stroke, acute coronary syndrome (acute myocardial infarction and hospitalization for unstable angina), acute decompensated heart failure, coronary revascularization, atrial fibrillation, or death from cardiovascular causes.

Results: Of the 9624 patients screened for eligibility, 8511 were enrolled in the trial; 4243 were randomly assigned to the intensive-treatment group and 4268 to the standard-treatment group. At 1 year of follow-up, the mean systolic blood pressure was 127.5 mm Hg in the intensive-treatment group and 135.3 mm Hg in the standard-treatment group. During a median follow-up period of 3.34 years, primary-outcome events occurred in 147 patients (3.5%) in the intensive-treatment group, as compared with 196 patients (4.6%) in the standard-treatment group (hazard ratio, 0.74; 95% confidence interval [CI], 0.60 to 0.92; P = 0.007). The results for most of the individual components of the primary outcome also favored intensive treatment: the hazard ratio for stroke was 0.67 (95% CI, 0.47 to 0.97), acute coronary syndrome 0.67 (95% CI, 0.47 to 0.94), acute decompensated heart failure 0.27 (95% CI, 0.08 to 0.98), coronary revascularization 0.69 (95% CI, 0.40 to 1.18), atrial fibrillation 0.96 (95% CI, 0.55 to 1.68), and death from cardiovascular causes 0.72 (95% CI, 0.39 to 1.32). The results for safety and renal outcomes did not differ significantly between the two groups, except for the incidence of hypotension, which was higher in the intensive-treatment group.

Conclusions: In older patients with hypertension, intensive treatment with a systolic blood-pressure target of 110 to less than 130 mm Hg resulted in a lower incidence of cardiovascular events than standard treatment with a target of 130 to less than 150 mm Hg. (Funded by the Chinese Academy of Medical Sciences and others; STEP ClinicalTrials.gov number, NCT03015311.).
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http://dx.doi.org/10.1056/NEJMoa2111437DOI Listing
September 2021

Surgical management for lumbar brucella spondylitis: Posterior versus anterior approaches.

Medicine (Baltimore) 2021 May;100(21):e26076

The Spinal Surgery Department of The First Affiliated Hospital Of University Of South China.

Abstract: There has been no ideal surgical approach for lumbar brucella spondylitis (LBS). This study aims to compare clinical efficacy and safety of posterior versus anterior approaches for the treatment of LBS.From April 2005 to January 2015, a total of 27 adult patients with lumbar brucella spondylitis were recruited in this study. The patients were divided into 2 groups according to surgical approaches. Thirteen cases in group A underwent 1-stage anterior debridement, fusion, and fixation, and 14 cases in group B underwent posterior debridement, bone graft, and fixation. The clinical and surgical outcomes were compared in terms of operative time, intraoperative blood loss, hospitalizations, bony fusion time, complications, visual analog scale score, recovery of neurological function, deformity correction.Lumbar brucella spondylitis was cured, and the grafted bones were fused within 11 months in all cases. It was obviously that the operative time and intraoperative blood loss of group A were more than those of group B (P = .045, P = .009, respectively). Kyphotic deformity was signifcantly corrected in both groups after surgery; however, the correction rate was higher in group B than in group A (P = .043). There were no significant differences between the two groups in hospitalizations, bony fusion time, and visual analog scale score in the last follow-up (P = .055, P = .364, P = .125, respectively).Our results suggested that both anterior and posterior approaches can effectively cure lumbar brucella spondylitis. Nevertheless, posterior approach gives better kyphotic deformity correction, less surgical invasiveness, and less complications.
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http://dx.doi.org/10.1097/MD.0000000000026076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154373PMC
May 2021

Promoting plaque stability by gene silencing of monocyte chemotactic protein-3 or overexpression of tissue factor pathway inhibitor in ApoE-/- mice.

J Drug Target 2021 07 1;29(6):669-675. Epub 2021 Feb 1.

Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Chemokines may promote the formation and instability of atherosclerotic plaque, which is the most common cause of acute coronary syndrome. The aim of this study was to clarify the function of monocyte chemotactic protein-3 (MCP-3) in the stability of atherosclerotic plaque, to determine the role of tissue factor pathway inhibitor (TFPI) on the development and stability of atherosclerotic plaques, and to further elucidate the anti-atherosclerotic mechanism of TFPI with the emphasis on chemokine MCP-3. We constructed an adenovirus-mediated shRNA against mouse MCP-3 (Ad-MCP-3-shRNA) and an adenovirus-containing TFPI (Ad-TFPI), and tranferred them in a model of vulnerable plaque in ApoE-/- mice respectively. Here, we reported that MCP-3-shRNA and TFPI could both reduce the plaque area and decrease the content of lipids and macrophages, on the contrary, the fibrous cap thickness and content of collagen and smooth muscle cells were increased. In addition, the expression of MCP-3 and CC chemokine receptor 2 (CCR2) was decreased by TFPI transfer. These data provide the first evidence that MCP-3 is a major contributor to the unstability of atherosclerotic plaque and TFPI may exert its anti-atherosclerotic effects and promote stabilisation of plaque at least partly through inhibiting MCP-3/CCR2 pathway, which may be a new therapeutic method for atherosclerosis.
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http://dx.doi.org/10.1080/1061186X.2021.1878363DOI Listing
July 2021

Inflammatory cytokines enhance procoagulant activity of platelets and endothelial cells through phosphatidylserine exposure in patients with essential hypertension.

J Thromb Thrombolysis 2021 May 21;51(4):933-940. Epub 2020 Nov 21.

Department of Cardiology, First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng Street, Nangang District, Harbin, 150001, Heilongjiang Province, China.

The exact mechanism of the prothrombotic state of essential hypertension (EH) patients remains elusive. Our objective was to assess whether phosphatidylserine (PS) exposure on endothelial cells (ECs), platelets, and microparticles (MPs) can account for the hypercoagulability in EH patients. PS exposure on cells and MPs, mainly from platelets and ECs was analyzed with flow cytometry. Procoagulant activity (PCA) was evaluated by purified coagulation complex assays, clotting time, and fibrin turbidity. We found that EH patients exhibited elevated levels of PS platelets, serum-cultured ECs, MPs, endothelial-derived MPs and platelet-derived MPs compared to the controls (all P < 0.05). Moreover, platelets and MPs from the patients and their sera-cultured ECs showed markedly enhanced intrinsic/extrinsic FXa, thrombin, and fibrin generation, and greatly shortened coagulation time. This PCA could be blocked approximately 80%, by the addition of lactadherin. Furthermore, we detected elevated levels of IL-8, IL-6, and TNF-α in EH patients could activate platelets/ECs and induce elevated PS exposure on their membranes. Our results suggest that inflammatory cytokines could enhance procoagulant activity of platelets and endothelial cells via their PS exposure in EH patients. As such, a PS blockade may be a viable therapeutic strategy for treating such patients.
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http://dx.doi.org/10.1007/s11239-020-02342-xDOI Listing
May 2021

Calhex231 ameliorates myocardial fibrosis post myocardial infarction in rats through the autophagy-NLRP3 inflammasome pathway in macrophages.

J Cell Mol Med 2020 11 12;24(22):13440-13453. Epub 2020 Oct 12.

Department of Cardiology, First Affiliated Hospital of Harbin Medical University, Harbin, China.

The calcium-sensing receptor (CaSR) is involved in the pathophysiology of many cardiovascular diseases, including myocardial infarction (MI) and hypertension. The role of Calhex231, a specific inhibitor of CaSR, in myocardial fibrosis following MI is still unclear. Using Wistar rats, we investigated whether Calhex231 ameliorates myocardial fibrosis through the autophagy-NLRP3 inflammasome pathway in macrophages post myocardial infarction (MI). The rats were randomly divided into sham, MI and MI + Calhex231 groups. Compared with the sham rats, the MI rats consistently developed severe cardiac function, myocardial fibrosis and infiltration of inflammatory cells including macrophages. Moreover, inflammatory pathway including activation of NLRP3 inflammasome, IL-1β and autophagy was significantly up-regulated in myocardial tissue, infiltrated cardiac macrophages and peritoneal macrophages of the MI rats. These impacts were reversed by Calhex231. In vitro, studies revealed that calindol and rapamycin exacerbated MI-induced autophagy and NLRP3 inflammasome activation in peritoneal macrophages. Calhex231 and 3-Methyladenine (a specific inhibitor of autophagy) attenuated both autophagy and NLRP3 inflammasome activation; however, the caspase-1 inhibitor Z-YVAD-FMK did not. Our study indicated that Calhex231 improved cardiac function and ameliorated myocardial fibrosis post MI, likely via the inhibition of autophagy-mediated NLRP3 inflammasome activation; this provides a new therapeutic target for ventricular remodelling-related cardiovascular diseases.
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http://dx.doi.org/10.1111/jcmm.15969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701583PMC
November 2020

Blockade of IL-6 alleviates bone loss induced by modeled microgravity in mice.

Can J Physiol Pharmacol 2020 Oct 13;98(10):678-683. Epub 2020 Aug 13.

Department of Spine Surgery, Hong Hui Hospital, Xi'an Jiaotong University, Xi'an, 710054, China.

This study investigated the effects of blockade of IL-6 on bone loss induced by modeled microgravity (MG). Adult male mice were exposed to hind-limb suspension (HLS) and treated with IL-6-neutralizing antibody (IL-6 nAb) for 4 weeks. HLS in mice led to upregulation of IL-6 expression in both sera and femurs. IL-6 nAb treatment in HLS mice significantly alleviated bone loss, evidenced by increased bone mineral density of whole tibia, trabecular thickness and number, bone volume fraction of proximal tibiae, and ultimate load and stiffness of femoral diaphysis. IL-6 nAb treatment in HLS mice significantly enhanced levels of osteocalcin in sera and reduced levels of deoxypyridinoline. In MC3T3-E1 cells exposed to MG in vitro, IL-6 nAb treatment increased mRNA expression and activity of alkaline phosphatase, mRNA expression of osteopontin and runt-related transcription factor 2, and protein levels of osteoprotegerin and decreased protein levels of receptor activator of the NF-κB ligand. In RAW254.7 cells exposed to MG, IL-6 nAb treatment downregulated mRNA expression of cathepsin K and tartrate-resistant acid phosphatase (TRAP) and reduced numbers of TRAP-positive multinucleated osteoclasts. In conclusion, blockade of IL-6 alleviated the bone loss induced by MG.
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http://dx.doi.org/10.1139/cjpp-2019-0632DOI Listing
October 2020

Cardioprotection of cortistatin against isoproterenol-induced myocardial injury in rats.

Ann Transl Med 2020 Mar;8(6):309

Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

Background: The present study was designed to examine whether cortistatin (CORT) could protect rats from myocardial injury induced by subcutaneously injecting isoproterenol (ISO) and to clarify the possible mechanisms.

Methods: Male Sprague-Dawley (SD) rats were placed at random into four groups: the control group, the ISO group, the ISO + CORT 25 µg/(kg·d) group, and the ISO + CORT 50 µg/(kg·d) group. Rat models of myocardial injury were established with the subcutaneous (s.c.) injections of 85 mg/kg ISO for 2 days. In the ISO+ CORT 25 µg/(kg·d) group and ISO+ CORT 50 µg/(kg·d) group, rats were given s.c. injections of CORT 25 µg/(kg·d) and CORT 50 µg/(kg·d) on the day before ISO, 3 days, respectively. Serum malondialdehyde (MDA) content, lactate dehydrogenase (LDH) activity, and creatine kinase isoenzyme (CK-MB) activity were measured by corresponding test kits. Western blot was applied to evaluate the expression of endoplasmic reticulum stress-related protein glucose regulatory protein 78 (GRP78), enhancer-binding protein homologous protein (CHOP), cysteinyl aspartate specific proteinase-12 (caspase-12), LC3-II, Beclin-1, and p62 in the rat myocardium.

Results: CORT alleviated the increased enzyme activities of serum LDH and CK-MB, and content of MDA (a typical marker of lipid peroxidation) in rats induced by ISO. CORT also prevented pathological myocardial injury in rats induced by ISO. Moreover, CORT attenuated the increased protein levels of GRP78, CHOP, and caspase-12, and reduced the increase of LC3-II, LC3-II/I, Beclin-1, and p62 in rats induced by ISO.

Conclusions: These data demonstrate that CORT can attenuate ISO-induced acute myocardial injury in rats likely by reducing lipid peroxidation, and inhibiting endoplasmic reticulum stress and autophagy. This supports CORT as a potentially being a new target for preventing and treating myocardial injury and its related disease.
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http://dx.doi.org/10.21037/atm.2020.02.93DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186754PMC
March 2020

Cortistatin ameliorates Ang II-induced proliferation of vascular smooth muscle cells by inhibiting autophagy through SSTR3 and SSTR5.

Life Sci 2020 Jul 26;253:117726. Epub 2020 Apr 26.

Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address:

Aims: Vascular smooth muscle cell (VSMC) proliferation plays a significant role in the development of various vascular disorders. However, the effect of cortistatin (CST) on VSMC proliferation remains unclear. Therefore, the purpose of our research aimed to study whether CST protected VSMCs from angiotensin II (Ang II)-induced proliferation and which mechanisms participated in the process.

Main Methods: Cultured rat VSMCs were treated with Ang II with or without CST for 24 h. Cell proliferation rate was measured by cell counting kit-8 (CCK8) assay. The expressions of CST and its receptors were assessed by quantitative real-time PCR (qRT-PCR). The protein expression levels were analyzed by western blots. Immunofluorescence and transmission electron microscopy (TEM) were used to observe autophagy.

Key Findings: Our results showed that different concentrations of CST alleviated the Ang II-induced VSMC proliferation. The autophagy and reactive oxygen species (ROS) stimulated by Ang II were attenuated by CST. Furthermore, when the autophagy inhibitor 3-methyladenine (3-MA) was added, it exerted similar inhibition effects like CST, but didn't augment the protective role of CST on Ang II-induced VSMC autophagy and proliferation. Moreover, blocking somatostatin receptor 3 and 5 (SSTR3 and SSTR5) partially abrogated the suppressive effect of CST on Ang II-stimulated VSMC proliferation and autophagy.

Significance: This study indicated that CST could ameliorate Ang II-stimulated VSMC proliferation by inhibiting autophagy partially through its receptors SSTR3 and SSTR5, providing a reasonable evidence for CST as a novel perspective therapeutic target of vascular diseases.
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http://dx.doi.org/10.1016/j.lfs.2020.117726DOI Listing
July 2020

Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration.

Int J Pharm 2020 May 5;582:119303. Epub 2020 Apr 5.

Department of Spine Surgery, Hong Hui Hospital, Xi'an Jiaotong University College of Medicine, No. 76 Nanguo Road, Beilin District, Xi'an, Shaanxi Province 710054, China. Electronic address:

Osteogenic differentiation is great significance for improving the bone regeneration. Present study evaluates the osteogenic ability of lanthanum (La) and silicate (SiO) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant. The bio-ceramic MHAP was synthesized by hydrothermal process with assistance of calcium alginate template. For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method. The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix. Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques. A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method. The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs). The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.
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http://dx.doi.org/10.1016/j.ijpharm.2020.119303DOI Listing
May 2020

Calcium-Sensing Receptor on Neutrophil Promotes Myocardial Apoptosis and Fibrosis After Acute Myocardial Infarction via NLRP3 Inflammasome Activation.

Can J Cardiol 2020 06 1;36(6):893-905. Epub 2019 Nov 1.

Department of Cardiology, First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address:

Background: The infiltration of neutrophils aggravates inflammatory response in acute myocardial infarction (AMI), and the role of calcium-sensing receptor (CaSR) in neutrophil-associated inflammation is largely unknown. The aim of this study was to evaluate the regulatory effects of CaSR on nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3 (NLRP3) inflammasome in neutrophils and to explore its role in AMI-related ventricular remodelling.

Methods: The expression of CaSR, NLRP3 inflammasome, and interleukin 1β (IL-1β) in peripheral blood and infiltrating neutrophils in patients and rats with AMI was detected by western blotting and immunofluorescence. Cardiomyocyte apoptosis was detected by western blotting and transmission electron microscopy. The degree of fibrosis was evaluated by Masson staining and western blotting.

Results: We found upregulation of CaSR, NLRP3 inflammasome, Caspase-1, and IL-1β in peripheral neutrophils from patients with AMI compared with matched healthy controls, peaking on day 1 and decreasing gradually till 7 days. Peripheral and infiltrating neutrophils from rats with AMI showed the same trend. Calindol enhanced NLRP3 inflammasome activation and IL-1β release in neutrophils from healthy volunteers, which was blocked by inhibitors of the PLC-IP pathway and ER-Ca release. Calhex-231 decreased NLRP3 inflammasome activation and IL-1β release in neutrophils from patients with AMI. The calindol-stimulated neutrophils from healthy rats promoted cardiomyocyte apoptosis and fibrosis of cardiac fibroblasts from healthy rats, which were inhibited by calhex-231.

Conclusion: The results suggest that CaSR activates NLRP3 inflammasome in neutrophils, contributing to ventricular remodelling after AMI. CaSR inhibition may be a potential therapeutic target for heart failure in AMI.
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http://dx.doi.org/10.1016/j.cjca.2019.09.026DOI Listing
June 2020

The association of metabolic syndrome components and chronic kidney disease in patients with hypertension.

Lipids Health Dis 2019 Dec 27;18(1):229. Epub 2019 Dec 27.

Cardiology Department, Huashan Hospital, Fudan University, No. 12 Mid Wulumuqi Road, Shanghai, 200040, China.

Background: Hypertension is a highly prevalent disease and the leading cause of chronic kidney disease (CKD). Metabolic syndrome could also be the risk factor for CKD. We sought to study the association between metabolic syndrome components and the prevalence of CKD in patients with hypertension.

Methods: We carried out a multi-center cross-sectional study from Apr. 2017- Apr. 2018 in 15 cities in China.

Results: A total of 2484 patients with hypertension were enrolled. Among them, 56% were male and the average age was 65.12 ± 12.71 years. The systolic BP/diastolic BP was 142 ± 18/83 ± 12 mmHg. Metabolic syndrome components turned out to be highly prevalent in patients with hypertension, ranging from 40 to 58%. The prevalence of chronic kidney disease reached 22.0%. Multi-variate logistic analysis revealed that elevated triglyceride (TG) (OR = 1.81, 95% CI 1.28-2.57, p < 0.01), elevated fasting blood glucose (FBG) (OR = 1.43, 95% CI 1.00-2.07, p = 0.05) and hypertension grades (OR = 1.20, 95% CI 1.00-1.44, p = 0.05) were associated with the prevalence of CKD. In sub-group analysis, elevated TG remained strongly associated with CKD in both diabetes (OR = 2.10, 95%CI 1.22-3.61, p < 0.01) and non-diabetes (OR = 1.53, 95% CI 1.09-2.16, p = 0.01). In sub-group analysis of hypertension grades, there was also a graded trend between elevated TG and CKD from controlled blood pressure (BP) to hypertension grade 2 (OR = 1.81, 95%CI 1.06-3.11, p = 0.03; OR = 1.85, 95%CI 1.00-3.43, p = 0.05; OR = 2.81, 95% CI 1.09-7.28, p = 0.03, respectively).

Conclusion: Elevated TG, elevated FBG and hypertension grades were significantly associated with the prevalence of CKD in patients with hypertension. Particularly, elevated TG was strongly associated with CKD, independent of diabetes and hypertension grades.
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http://dx.doi.org/10.1186/s12944-019-1121-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935087PMC
December 2019

Cortistatin exerts antiproliferation and antimigration effects in vascular smooth muscle cells stimulated by Ang II through suppressing ERK1/2, p38 MAPK, JNK and ERK5 signaling pathways.

Ann Transl Med 2019 Oct;7(20):561

Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

Background: Vascular remodeling, that contributes to cardiovascular diseases such as hypertension develops by anomalous proliferation and migration of vascular smooth muscle cells (VSMCs). Cortistatin (CST), a newly discovered biological peptide, has been acknowledged for its protective effects against cardiovascular diseases. Whether CST has an inhibitory regulation role in angiotensin II (Ang II)-induced proliferation and migration of VSMCs and what molecular mechanisms may participate in the CST inhibition process are still unknown.

Methods: VSMCs were divided into control group, Ang II (10 M) group, Ang II + PD98059 (5×10 M) group, Ang II + SB203580 (10 M) group, Ang II + SP600125 (10 M) group, Ang II + XMD17-109 (10 M) group, Ang II + CST (10 M) group and Ang II + CST (10 M) group. Cell proliferation was detected by western blotting and cell counting kit-8 (CCK8) analysis. Migration of VSMCs was measured by Transwell assay.

Results: Compared with control group, Ang II upregulated the expression levels of proliferating cell nuclear antigen (PCNA) and osteopontin (OPN) and downregulated that of α-smooth muscle actin (α-SMA), increased the proliferation rate as shown by CCK8 and VSMC migration as shown by Transwell assay in cultured VSMCs of the Ang II group. Meanwhile, in Ang II-cultured VSMCs, we found activation of extracellular signal-regulated kinase (ERK) 1/2, p38 MAP kinase (p38 MAPK), c-Jun N-terminal kinase (JNK), and ERK5 pathways by western blotting at different time points. However, the proliferation and migration stimulated by Ang II were partly reversed by drug inhibitors of the four pathways, namely, PD98059, SB203580, SP600125 and XMD17-109. When Ang II-stimulated VSMCs were cultured with CST pretreatment, we found that proliferation and migration were greatly suppressed as well as that the ERK1/2, p38 MAPK, JNK and ERK5 pathways were deactivated by CST.

Conclusions: The accumulated data suggest that CST may play a protective role in Ang II-promoted proliferation and migration of VSMCs via inhibiting the mitogen-activated protein kinase (MAPK) family pathways, providing a new orientation of CST in protecting against cardiovascular diseases.
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http://dx.doi.org/10.21037/atm.2019.09.45DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6861776PMC
October 2019

Characterization and Interpretation of Cd (II) Adsorption by Different Modified Rice Straws under Contrasting Conditions.

Sci Rep 2019 11 28;9(1):17868. Epub 2019 Nov 28.

Institute of Agriculture, Jilin Agricultural Science and Technology University, Jilin, 132101, China.

Rice straw can adsorb Cd(II) from wastewater, and modification of rice straw may improve its adsorption efficiency. The rice straw powder (Sp) from the direct pulverization of rice straw was used as the control, the rice straw ash (Sa), biochar (Sa), and modified rice straw (Ms) were prepared by ashing, pyrolysis and citric acid modification, respectively, and all of them were examined as adsorbents for Cd(II) in this study. Batch adsorption experiments were adopted to systematically compare the adsorption capacities of rice straw materials prepared with different modification methods for Cd(II) from aqueous solution under different levels of initial Cd(II) concentration (0-800 mg·L), temperature (298, 308, and 318 K), contact time (0-1440 min), pH value (2-10), and ionic strength (0-0.6 mol·L). The results indicated that the modification method affected the adsorption of Cd(II) by changing the specific surface area (SSA), Si content, surface morphology, and O-containing functional group of rice straw. Compared with Sp, Ms held more surface O-H, aliphatic and aromatic groups, while Sa had more phenolic, C-O (or C-O-C), and Si-O groups, and Sb held more C-O (or C-O-C) and Si-O groups; besides, Sa, Sb, and Ms had larger SSA than Sp. Adsorption capacity of the four adsorbents for Cd(II) increased and gradually became saturated with the increase in the initial Cd(II) concentration (0-800 mg·L). The adsorption capacity of Cd(II) was significantly higher at 318 K than 298 K and 308 K, regardless of the adsorbent type. Sa had the largest SSA (192.38 m·g) and the largest adsorption capacity for Cd(II). When the initial Cd concentration was at 800 mg·L, the Cd(II) adsorption amount reached as high as 68.7 mg·g with Sa at 318 K. However, the SSA of Sp was only 1.83 m·g, and it had the least adsorption capacity for Cd(II). Only the adsorption of Cd(II) upon Sb at 298 K was spontaneous, and surprisingly, all other adsorptions were nonspontaneous. These adsorptions were all chemical, and were favorable, exothermic and order-increasing processes. The pseudo-second-order model showed a strong fit to the kinetics of Cd(II) adsorption by the four adsorbents. The adsorption capacities of Cd(II) by the adsorbents were less at low pH, and all were enhanced with the increase of initial pH value (2-10) in the solution. The inhibiting effect on Cd(II) adsorption due to the increase in ionic strength was greater with Sa, Sb, and Ms than that under Sp. The rice straw ash prepared by ashing unexpectedly had greater adsorption capacity for Cd(II) than the biochar and citric acid modified rice straw. The optimum condition for Cd(II) adsorption was established as the temperature of 318 K, initial Cd(II) concentration of 800 mg·L, contact time of 240 min, and no Na(I) interference regardless of absorbent. In conclusion, rice straw ash shows the greatest potential of being applied to paddy fields for the remediation of Cd(II) pollution so as to reduce the risk of Cd(II) enrichment in rice grains and straws.
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http://dx.doi.org/10.1038/s41598-019-54337-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882831PMC
November 2019

Cd(II) Adsorption on Different Modified Rice Straws under FTIR Spectroscopy as Influenced by Initial pH, Cd(II) Concentration, and Ionic Strength.

Int J Environ Res Public Health 2019 10 26;16(21). Epub 2019 Oct 26.

College of Agriculture, Jilin Agricultural Science and Technology University, Jilin 132101, China.

Rice straw is a kind of low-cost biosorbent. Through mechanical crushing, pyrolysis, incineration, and citric acid (CA) modification, it could be converted to rice straw powder (Sp), biochar (Sb), ash (Sa), and modified rice straw (Ms) accordingly. Using rice straw as an adsorbent, the influence of pH value (2, 4, and 9), initial Cd(II) concentration (0, 200, and 800 mg/L), and ionic strength (0, 0.2, to 0.6 mg/L) on the adsorption capacity for Cd(II) were examined with three replicates, and the relevant mechanisms were explored using Fourier transform infrared (FTIR) technology. Results showed that the modifications could improve the adsorption capacity of Cd(II) by changing their chemical structures. The products (Sb and Sa) of the pyrolysis and incineration of rice straw contained fewer hydroxyl and alkyl groups, but more Si-O groups. Citric acid modification removed a portion of silica in rice straw, increased its carboxylic content, and made more -OH groups exposed. Compared with Sp, Sb, Sa, and Ms were more likely to act as donors in the Cd(II) sorption process and exhibited more carboxyl binding. The bands of C = C, -O-CH, and the O-H, carboxyl, Si-O-Si or Si-O groups were involved in the Cd(II) sorption process. The adsorption amount of Cd(II) by the four adsorbents increased with the increase in the pH value of the solution and the initial Cd(II) concentration. Affected by pH in a solution, ion exchange, surface complexation, and precipitation were the major adsorption mechanisms. Further, under the influence of the initial Cd(II) concentration, electrostatic attraction played a leading role. With no interference by ionic strength, all the adsorbents had the greatest adsorption amount of Cd(II), and the intensity of O-H vibration was also the weakest; ion exchange was the most important mechanism in this process. Regardless of the influencing factors, Sa, with the greatest specific surface area, had an absolute advantage in the adsorption capacity of Cd(II) over Sp, Sb, and Ms.
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http://dx.doi.org/10.3390/ijerph16214129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862161PMC
October 2019

Cortistatin, a novel cardiovascular protective peptide.

Cardiovasc Diagn Ther 2019 Aug;9(4):394-399

Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

Cortistatin (CST) is a small molecule bioactive peptide containing an FWKT tetramer. It is widely distributed in nervous, immune and endocrine systems. Many studies have shown that CST can exert many biological effects, for example: regulating sleep, learning and memory processes, inducing immune tolerance, inhibiting inflammatory responses, and regulating endocrine metabolism. Notably, it is found that CST and its receptors are also widely distributed in the cardiovascular system, such as the aorta, coronary arteries and heart. In recent years, increasing studies have shown that CST played an important role in the development of cardiovascular diseases, such as reducing myocardial damage, inhibiting autoimmune myocarditis, alleviating vascular smooth muscle cell (VSMC) proliferation and migration, reducing vascular calcification (VC), and inhibiting atherosclerosis and aneurysm formation. Therefore, we reviewed the cardiovascular effects of CST in the heart and blood vessels, which will help to understand the role of CST and its receptors in the pathogenesis of cardiovascular diseases, and highlight novel strategies and targets for the prevention and treatment of cardiovascular diseases.
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http://dx.doi.org/10.21037/cdt.2018.12.08DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732083PMC
August 2019

Letter: Ultra-Early (<12 Hours) Surgery Correlates With Higher Rate of American Spinal Injury Association Impairment Scale Conversion After Cervical Spinal Cord Injury.

Neurosurgery 2019 08;85(2):E399-E400

Department of Spine Surgery Hong-Hui Hospital Xi'an Jiaotong University College of Medicine Xi'an, China.

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http://dx.doi.org/10.1093/neuros/nyz154DOI Listing
August 2019

Ammonia Volatilization Loss and Corn Nitrogen Nutrition and Productivity with Efficiency Enhanced UAN and Urea under No-tillage.

Sci Rep 2019 04 29;9(1):6610. Epub 2019 Apr 29.

Associate Professor and Full Professor, Department of Biosystems Engineering and Soil Science, The University of Tennessee, 2506 E. J. Chapman Drive, Knoxville, TN, 37996, USA.

New urease and nitrification inhibitors and polymer coatings were introduced in recent years, but their effects on N loss and plant N nutrition were scarcely examined in agronomic no-tillage production systems. A field experiment of urea treated with efficiency enhancers was conducted on no-tillage corn (Zea mays L.) in Tennessee, the USA during 2013-2015. A field experiment on urea and ammonium nitrate (UAN) treated with efficiency enhancers was carried out on no-tillage corn in Tennessee in 2014 and 2015. Urea treated with N-(n-butyl) thiophosphoric triamide (NBPT) at concentrations of 20% (NBPT), 26.7% (NBPT), or 30% (NBPT) and polymer coated urea (PCU) were effective but maleic-itaconic copolymer treated urea was ineffective in reducing ammonia volatilization loss and improving N nutrition, grain yield, and N agronomic use efficiency of corn compared with untreated urea. Specifically, NBPT, NBPT, or NBPT treated urea and PCU reduced the total ammonia volatilization loss by 29.1-78.8%, 35.4-81.9%, 77.3-87.4%, and 59.1-83.3% during the 20 days after N applications, but increased grain yield by 15.6-31.4%, 12.9-34.8%, 18.7-19.9%, and 14.6-41.1%, respectively. The inhibitory effect of NBPT on ammonia volatilization did not improve with NBPT concentration increased from 20% to 30%. UAN treated with NBPT or a combination of urease and nitrification inhibitors resulted in 16.5-16.6% higher corn yield than untreated UAN only when they were surface applied. In conclusion, when urea-containing fertilizers are surface applied without any incorporation into the soil under no-tillage, their use efficiencies and performances on corn can be enhanced with an effective urease inhibitor in areas and years with noticeable urea N losses.
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http://dx.doi.org/10.1038/s41598-019-42912-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488641PMC
April 2019

H2 relaxin ameliorates angiotensin II-induced endothelial dysfunction through inhibition of excessive mitochondrial fission.

Biochem Biophys Res Commun 2019 05 27;512(4):799-805. Epub 2019 Mar 27.

Department of Cardiology, First Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang Province, China. Electronic address:

The physiological function of endothelial cells plays an important role in maintaining normal cardiovascular function. Endothelial dysfunction induced by AngII (angiotensin II) is the pathological mechanism of occurrence and development of cardiovascular diseases. Human recombinant relaxin-2 (H2 relaxin), which has protective effect on cardiovascular functions, ameliorates damage to endothelial cells induced by angiotensin II (AngII) treatment. However, the exact mechanisms remain unclear. In this study, we researched the mechanisms of H2 relaxin inhibiting AngII-induced endothelial dysfunction from the protective effect of H2 relaxin on endothelial function though inhibiting excessive mitochondrial fission. Here, we found that H2 relaxin increased eNOS, SOD1 expression, inhibited excessive mitochondrial fission and decreased ROS level in HUVECs treated with AngII. However, overexpression of fission protein 1 (Fis1) prevented H2 relaxin from protecting against AngII-induced low eNOS, SOD1 expression, excessive mitochondrial fission and increased ROS level in HUVECs. Our study indicated that excessive mitochondrial fission could be a target for H2 relaxin to treat endothelial dysfunction in angiocardiopathy.
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http://dx.doi.org/10.1016/j.bbrc.2019.03.112DOI Listing
May 2019

NLRP3 Inflammasome Is Involved in Calcium-Sensing Receptor-Induced Aortic Remodeling in SHRs.

Mediators Inflamm 2019 13;2019:6847087. Epub 2019 Feb 13.

Department of Cardiology, First Affiliated Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, China.

Increasing evidence suggests that the NLRP3 (nucleotide oligomerization domain-like receptor family, pyrin domain containing 3) inflammasome participates in cardiovascular diseases. However, its role and activation mechanism during hypertension remains unclear. In this study, we tested the role and mechanism of calcium-sensing receptor (CaSR) in NLRP3 inflammasome activation during hypertension. We observed that the expressions of CaSR and NLRP3 were increased in spontaneous hypertensive rats (SHRs) along with aortic fibrosis. In vascular smooth muscle cells (VSMCs), the activation of NLRP3 inflammasome associated with CaSR and collagen synthesis was induced by angiotensin II (Ang II). Furthermore, inhibition of CaSR and NLRP3 inflammasome attenuated proinflammatory cytokine release, suggesting that CaSR-mediated activation of the NLRP3 inflammasome may be a therapeutic target in aortic dysfunction and vascular inflammatory lesions.
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http://dx.doi.org/10.1155/2019/6847087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393924PMC
July 2019

Interactive influences of intercropping by nitrogen on flavonoid exudation and nodulation in faba bean.

Sci Rep 2019 03 18;9(1):4818. Epub 2019 Mar 18.

College of Resource and Environmental Science, Yunnan Agricultural University, Kunming, 650201, China.

In order to address the question of how flavonoids affected root nodulation of faba bean in a wheat and faba bean intercropping system, we set up soil and hydroponic experiments comprising two cropping pattern treatments (intercropped and monocropped) and three nitrogen (N) supply treatments at the deficient (50% N), adequate (100% N), and excessive (150% N) levels with three replicates in a randomized complete block design. Across the three N treatments and two experiments, it was frequently observed that intercropping increased but N fertilization decreased the nodule number and nodule dry weight of faba bean. Six types of flavonoids were detected in the faba bean root secretion, but only genistein, hesperetin, and naringenin often had significant correlations with the nodule number and nodule dry weight. Intercropping increased faba bean root secretions of genistein, hesperetin, and naringenin compared to monoculture only at the deficient and adequate N supply levels. The differences in flavonoids of faba bean caused by the intercropped patterns, N supply levels, and their interactions were mainly significant at flowering stage. In conclusion, interspecies and N supply interactively altered the contents and proportions of flavonoids in faba bean root exudations under wheat and faba bean intercropping. These findings provide insight into flavonoids-nodule-yield interactions in cereal and legume intercropping systems.
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http://dx.doi.org/10.1038/s41598-019-41146-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423034PMC
March 2019

Treatment with hydrogen sulfide donor attenuates bone loss induced by modeled microgravity.

Can J Physiol Pharmacol 2019 Jul 14;97(7):655-660. Epub 2019 Mar 14.

Department of Spine Surgery, Hong Hui Hospital, Xi'an Jiaotong University, Xi'an, 710054, China.

The present study was undertaken to explore the therapeutic potential of hydrogen sulfide against bone loss induced by modeled microgravity. Hindlimb suspension (HLS) and rotary wall vessel bioreactor were applied to model microgravity in vivo and in vitro, respectively. Treatment of rats with GYY4137 (a water soluble donor of hydrogen sulfide, 25 mg/kg per day, i.p.) attenuated HLS-induced reduction of bone mineral density in tibiae, and preserved bone structure in tibiae and mechanical strength in femurs. In HLS group, GYY4137 treatment significantly increased levels of osteocalcin in sera. Interestingly, treatment of HLS rats with GYY4137 enhanced osteoblast surface, but had no significant effect on osteoclast surface of proximal tibiae. In MC3T3-E1 cells exposed to modeled microgravity, GYY4137 stimulated transcriptional levels of runt-related transcription factor 2 and enhanced osteoblastic differentiation, as evidenced by increased mRNA expression and activity of alkaline phosphatase. HLS in rats led to enhanced levels of interleukin 6 in sera, skeletal muscle, and tibiae, which could be attenuated by GYY4137 treatment. Our study showed that GYY4137 preserved bone structure in rats exposed to HLS and promoted osteoblastic differentiation in MC3T3-E1 cells under modeled microgravity.
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http://dx.doi.org/10.1139/cjpp-2018-0521DOI Listing
July 2019

FBXL10 regulates cardiac dysfunction in diabetic cardiomyopathy via the PKC β2 pathway.

J Cell Mol Med 2019 04 31;23(4):2558-2567. Epub 2019 Jan 31.

Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.

Diabetic cardiomyopathy (DCM) is a condition associated with significant structural changes including cardiac tissue necrosis, localized fibrosis, and cardiomyocyte hypertrophy. This study sought to assess whether and how FBXL10 can attenuate DCM using a rat streptozotocin (STZ)-induced DCM model system. In the current study, we found that FBXL10 expression was significantly decreased in diabetic rat hearts. FBXL10 protected cells from high glucose (HG)-induced inflammation, oxidative stress, and apoptosis in vitro. In addition, FBXL10 significantly activated PKC β2 signaling pathway in H9c2 cells and rat model. The cardiomyocyte-specific overexpression of FBXL10 at 12 weeks after the initial STZ administration attenuated oxidative stress and inflammation, thereby reducing cardiomyocyte death and preserving cardiac function in these animals. Moreover, FBXL10 protected against DCM via activation of the PKC β2 pathway. In conclusion, FBXL has the therapeutic potential for the treatment of DCM.
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http://dx.doi.org/10.1111/jcmm.14146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433654PMC
April 2019

Bilateral costotransverse and local continuous chemotherapy approach for debridement, fixation, and fusion of contiguous multisegmental thoracic spinal tuberculosis: A retrospective study.

Medicine (Baltimore) 2018 Oct;97(41):e12752

Department of Spine Surgery, Hong Hui Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, China.

The study aims to evaluate the clinical efficacy of bilateral costotransverse debridement, transpedicular fixation, fusion, and local continuous chemotherapy in 20 patients of contiguous multisegmental thoracic spinal tuberculosis (CMTSTB). We analyzed 20 patients with contiguous thoracic spinal tuberculosis (TB) who underwent surgery via bilateral costotransverse debridement, fusion, posterior instrumentation, and postural drainage with local continuous chemotherapy. The clinical outcomes were evaluated in terms of kyphotic angle, bone fusion, neurologic status, erythrocyte sedimentation rate (ESR), and intraoperative and postoperative complications. All of the patients (8M/12F), averaged 45.8 ± 15.6 years old. The mean duration of postoperative follow-up was 30.7 ± 4.0 months. There was no recurrent TB infection. The values of ESR returned to normal levels at final follow-up. All patients got bony fusion within 8.1 ± 2.3 months after surgery. The average preoperative Cobb angle was 39.9° ± 8.6°, correcting to 9.8° ± 2.3° postoperatively and 10.8° ± 2.3° at the last follow-up. All patients with neurological deficit had dramatic improvement at the final follow-up. Our results showed that bilateral costotransverse surgery and local continuous chemotherapy are feasible and effective procedures in the treatment of CMTSTB. The approach can provide radical debridement, rebuild spinal stability, and cure TB.
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http://dx.doi.org/10.1097/MD.0000000000012752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203528PMC
October 2018

Nox4-dependent ROS production is involved in CVB-induced myocardial apoptosis.

Biochem Biophys Res Commun 2018 09 23;503(3):1641-1644. Epub 2018 Jul 23.

Department of Cardiology, First Affiliated Hospital of Harbin Medical University, No. 199 Dazhi Street, Harbin, 150001, China. Electronic address:

Viral myocarditis is a cardiovascular disease that seriously affects human health. Its mechanism is not clear. Coxsackievirus B3 (CVB3) is a member of the picornavirus family and is the leading cause of viral myocarditis. Our group tested the genes in a mouse model of CVB3 virus infection and confirmed that the NADPH oxidase gene had a high expression trend in the acute phase of infection. Whether Nox4, the homologue of NADPH oxidase, participates in the process of viral myocarditis has not been reported. In this study, we found increased expression of Nox4 in viral myocarditis in vivo and in vitro. DPI is a non-specific inhibitor of Nox4 that improved CVB3-induced myocarditis after injection in vivo. DPI also inhibited intracellular ROS release and apoptosis in vitro. Our data indicated that Nox4-dependent ROS production was involved in CVB3-induced myocardial apoptosis.
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http://dx.doi.org/10.1016/j.bbrc.2018.07.093DOI Listing
September 2018

Comparison of clinical results between novel percutaneous pedicle screw and traditional open pedicle screw fixation for thoracolumbar fractures without neurological deficit.

Int Orthop 2019 07 16;43(7):1749-1754. Epub 2018 Jun 16.

Department of Spinal Surgery, Hong Hui Hospital, Xi'an Jiaotong University College of Medicine, 555 Friendship East Road, Xi'an, 710054, Shanxi, China.

Objectives: To compare the efficacy and safety of novel percutaneous minimally invasive pedicle screw fixation and traditional open surgery for thoracolumbar fractures without neurological deficit.

Methods: Sixty adult patients with single thoracolumbar fracture between June 2014 and June 2016 were recruited in this study, randomly divided into open fixation group (group A) or minimally invasive percutaneous fixation group (group B). Clinical and surgical evaluation including surgery time, blood losses, radiation times, hospital stay, and complication were performed. The two groups of patients with pre-operative and last follow-up anterior height ratio of fracture vertebral, Cobb angle of fracture vertebral, and VAS score of back pain were compared.

Results: All patients completed valid follow-ups, with an average time period of 15.4 months (12-26 months). Group B achieved much better results in time of operation, intra-operative blood loss, and length of stay than group A (P < 0.05). Group A was significantly better than group B in the times of radiation (P < 0.05). The VAS score was significantly lower in group B than in group A at three days after the operation (P < 0.05). There were no significant differences between the two groups in the anterior height ratio of fracture vertebral, Cobb angle, and VAS score in the last follow-up (P > 0.05). No injured nerve or other severe complications occurred in both groups; one of the patients from group A had back and loin pain lasting for about one month, which resolved after analgesia and functional training. There was no significant difference between the two groups in incidence of complications.

Conclusions: Novel percutaneous pedicle screws with angle reset function can achieve the same effect as traditional open pedicle screw fixation in the treatment of thoracolumbar fractures without nerve injuries. Percutaneous minimally invasive pedicle screw fixation has the characteristics of shorter operative time, less bleeding, and less pain, but it needs more radiation times.
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http://dx.doi.org/10.1007/s00264-018-4012-xDOI Listing
July 2019

Corrigendum: H3 relaxin inhibits the collagen synthesis via ROS- and P2X7R-mediated NLRP3 inflammasome activation in cardiac fibroblasts under high glucose.

Authors:
Xinhua Yin

J Cell Mol Med 2018 Jun;22(6):3264-3265

The Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

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http://dx.doi.org/10.1111/jcmm.13615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980173PMC
June 2018

Cyclosporin A induces autophagy in cardiac fibroblasts through the NRP-2/WDFY-1 axis.

Biochimie 2018 May 1;148:55-62. Epub 2018 Mar 1.

Department of Cardiology, First Affiliated Hospital of Harbin Medical University, No. 199 Dazhi Street, Harbin 150001, China. Electronic address:

Cyclosporin A (CsA) is an effective immunosuppressive agent, but its myocardial toxicity limits its widespread and long-term clinical application. In this study, CsA treatment led to damages in myocardial fiber structure, an increase in myocardial fibrosis, and changes in heart size and shape; moreover, the degree of damage was exacerbated with prolonged drug application and increases in dose. However, the mechanism is not clear; therefore, the purpose of this study was to reveal the mechanism of CsA-induced myocardial fibrosis and identify a new target for the prevention and treatment of CsA-induced myocardial injury. Cardiac fibroblasts were treated with CsA (5, 10, or 20 μg/mL) for 24 h. Autophagy was observed by electron microscopy and immunofluorescence. The expression of NRP-2/WDFY-1, autophagy-related proteins (Beclin1 and LC3B), fibrosis-related proteins (MMP2/9), and fibroblast phenotype conversion factor (α-SMA) was evaluated by Western blot. The expression of collagen I was determined by ELISA. Then, we used the gene interference technique to alter WDFY-1 expression with or without CsA or 3-MA treatment for 24 h, and the effects on autophagy and the expression of autophagy-related proteins, fibrosis-associated proteins, IFN-α, TNF-α, and IL-6 were determined. The results showed the following: (1) CsA induced fibrosis-related protein (MMP2/9), fibroblast phenotype conversion factor (α-SMA), and collagen I up-regulation in a dose-dependent manner. (2) CsA induced the formation of autophagosomes and up-regulated the expression of Beclin1, LC3B, and the ERK/MAPK pathway in cardiac fibroblasts. (3) CsA induced NRP-2 down-regulation and WDFY-1 up-regulation. (4) Depletion of WDFY-1 inhibited CsA-induced autophagy, TNF-α and IFN-α up-regulation, and fibrosis. (5) The autophagy inhibitor 3-MA inhibited CsA-induced TNF-α and IFN-α up-regulation and fibrosis. Overall, cyclosporin A induces autophagy in cardiac fibroblasts through the NRP-2/WDFY-1 axis, which promotes the progression of myocardial fibrosis.
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http://dx.doi.org/10.1016/j.biochi.2018.02.017DOI Listing
May 2018

Activation of calcium-sensing receptor-mediated autophagy in angiotensinII-induced cardiac fibrosis in vitro.

Biochem Biophys Res Commun 2018 03 13;497(2):571-576. Epub 2018 Feb 13.

Department of Cardiology, First Affiliated Hospital of Harbin Medical University, No. 199 Dazhi Street, Harbin 150001, China. Electronic address:

Cardiac fibrosis is one of the primary mechanisms of ventricular remodeling, and there is no effective method for reversal. Activation of calcium sensing receptor (CaSR) has been reported to be involved in the development of myocardial fibrosis, but the molecular mechanism for CaSR activation has not yet been clarified and needs to be further explored. Here, we found that AngII induces cardiac fibroblast proliferation and phenotypic transformation in a dose-dependent manner with increased CaSR and autophagy related protein (Beclin1, LC3B) expression. CaSR activation results in intracellular calcium release, MEK1/2 pathway phosphorylation, autophagy activation and collagen formation induced by AngII in cardiac fibroblasts. However, pretreating the cells with Calhex, PD98059 or 3-MA partially blocked AngII-induced cardiac fibrosis. Our data indicate that the activation of CaSR-mediated MEK/ERK and autophagic pathways is involved in AngII-induced cardiac fibrosis in vitro.
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http://dx.doi.org/10.1016/j.bbrc.2018.02.098DOI Listing
March 2018
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