Publications by authors named "Xingxiang Wang"

77 Publications

Polymer-coated manganese fertilizer and its combination with lime reduces cadmium accumulation in brown rice (Oryza sativa L.).

J Hazard Mater 2021 Mar 6;415:125597. Epub 2021 Mar 6.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 71 East Beijing Road, Nanjing 210008, China; Ecological Experimental Station of Red Soil, Chinese Academy of Sciences, Yingtan 335211, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Manganese (Mn) has the potential to reduce cadmium (Cd) uptake by rice; however, the efficiency depends on its soil availability. Therefore, this study designed a slow-release Mn fertilizer by employing a polyacrylate coating. Pot trials were conducted to study the effects of coated-Mn and uncoated-Mn alone or in combination with lime on the dynamics of soil dissolved-Mn and available Cd, and the transportation of Mn and Cd within rice. The results showed that coated-Mn declined the release of Mn until the 7th day of application; however, it consistently supplied more dissolved-Mn than uncoated-Mn. As a result, coated-Mn induced a greater Cd reduction (45.8%) in brown rice than uncoated-Mn (9.7%). The total Cd of rice and its proportion in brown rice were greatly reduced by coated-Mn, indicating the inhibition of root uptake and interior transport of Cd. Additionally, lime addition prominently increased the soil pH and decreased the CaCl-extractable Cd (90.1-93.9%). However, since lime reduced the soil dissolved-Mn, downregulated the OsHMA3 expression and upregulated the OsNramp5 expression, brown rice Cd was reduced by only 43.0%. The combined addition of lime and coated-Mn alleviated the liming effect on soil Mn and gene expression in roots, thereby reducing brown rice Cd by 71.5%.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125597DOI Listing
March 2021

SERS-Microfluidic Approach for the Quantitative Detection of miRNA Using DNAzyme-Mediated Reciprocal Signal Amplification.

ACS Sens 2021 03 16;6(3):1392-1399. Epub 2021 Feb 16.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.

MicroRNAs (miRNAs) play important roles in biological processes. Designing a sensitive, selective, and rapid method of miRNA detection is crucial for biological research. Here, with a reciprocal signal amplification (RSA) probe, this work established a novel surface-enhanced Raman scattering (SERS)-microfluidic approach for the quantitative analysis of miRNA. First, via a DNAzyme self-assemble cycle reaction, two types of SERS signals produce amplified reciprocal changes. The sum of the absolute signal value is first adopted for the quantitative analysis of miRNA, which results in an enhanced response and a reduced blank value. Furthermore, the assay is integrated in an electric drive microfluidic mixing reactor that enables physical mixing and enriching of the reactants for more rapid and enhanced detection sensitivity. The protocol owns the merits of the SERS technology, amplified reciprocal signals, and a microfluidic chip, with a detection limit of 2.92 fM for miR-141 in 40 min. In addition, successful determination of miRNA in a variety of cells proved the practicability of the assay. Compared with the reported strategies for miRNA analysis, this work avoids a complex and time-consuming procedure and enhances the sensitivity and specificity. The method opens a promising way for biomolecular chip detection and research.
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http://dx.doi.org/10.1021/acssensors.1c00063DOI Listing
March 2021

Aging of exogenous arsenic in flooded paddy soils: Characteristics and predictive models.

Environ Pollut 2021 Apr 22;274:116561. Epub 2021 Jan 22.

CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. Electronic address:

Understanding the arsenic (As) aging process is important for predicting the environmental behavior of exogenous As in paddy soils. In this work, samples of sixteen paddy soils with various soil properties were spiked with two concentrations (30 and 100 mg kg) of arsenate and subjected to a 360 day-long incubation under continuous flooding condition. Soil available As extracted by 0.05 M NHHPO was monitored through the aging process. Results showed that the available As%, the percentage of remaining available As in aged soils to added total As, fell from 44.2% to 41.9% on the 1st day to 22.0% and 23.0% on the 115th day for the low and high As spiked soils, respectively, then it remained basically unchanged after the 115th day. The pseudo-second order equation could adequately describe the aging kinetics of exogenous As in paddy soils. There was no significant difference in As aging parameters between the two spiked concentrations. Contents of soil free Al and Mn oxides, clay and cation exchange capacity strongly affected the aging rate of exogenous As. An empirical model, incorporating soil pH, cation exchange capacity, Olsen-P and flooding time, was developed to predict well the change of soil available As% during aging process (R = 0.711). The model could be potentially utilized to manage As-contaminated paddy fields and normalize ecotoxicity and bioaccumulation datasets in attempt to derive more widely applicable soil environmental quality criteria for As.
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http://dx.doi.org/10.1016/j.envpol.2021.116561DOI Listing
April 2021

Pollution characteristics of livestock faeces and the key driver of the spread of antibiotic resistance genes.

J Hazard Mater 2021 May 29;409:124957. Epub 2020 Dec 29.

CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; Ecological Experimental Station of Red Soil, Chinese Academy of Sciences, Yingtan 335211, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

The increasing prevalence of antibiotic resistance genes (ARGs) in livestock and poultry faeces has attracted considerable amounts of attention. However, in the actual breeding environment, the key driver of the spread of ARGs and which bacteria are involved remain unclear. This study investigated 19 antibiotics and 4 heavy metals in 147 animal faeces. The results showed that piglet faeces exhibited the highest levels of antibiotics and heavy metals. Twelve ARGs, 4 mobile genetic elements (MGEs) and bacterial communities of piglet faeces from 6 pig farms were further assessed to determine the key driver and relevant mechanism of the spread of ARGs. Sulphonamides (SAs) explained 36.5% of the variance (P < 0.05) of the bacterial community and were significantly related to 8 genes (P < 0.01), indicating that SAs dominated the spread of ARGs and should be tightly supervised. Structural equation modelling (SEM) indicated that SAs increased the abundance of ARGs via two pathways: horizontal transfer of ARGs (involving 10 genera) and vertical transfer of ARGs (involving 26 genera). These results improve our understanding of the potential hosts involved in the spread of ARGs, suggesting that monitoring of the above potential hosts is also important in animal feeding practice.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124957DOI Listing
May 2021

Fluorescent-Raman Binary Star Ratio Probe for MicroRNA Detection and Imaging in Living Cells.

Anal Chem 2021 01 21;93(3):1466-1471. Epub 2020 Dec 21.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; Shandong Key Laboratory of Biochemical Analysis; College of Chemistry and Molecular Engineering. Qingdao University of Science and Technology, Qingdao 266042, P. R. China.

The expression of microRNAs (miRNAs) is critical in gene regulation and has been counted into disease diagnosis marks. Precise imaging and quantification of miRNAs could afford the important information for clinical diagnosis. Here, two smart binary star ratio (BSR) probes were designed and constructed, and miRNA triggered the connection of the binary star probes and the reciprocal changes of dual signals in living cells. This multifunctional probe integrates fluorescence and surface enhanced Raman scattering (SERS) imaging, with enzyme-free numerator signal amplification for dual-mode imaging and dual-signal quantitative analysis of miRNA. First, compared with the single-mode ratio imaging method, using fluorescence-SERS complementary ratio imaging, this probe enables more accurate imaging contrast for direct visualization signal changes in living cells. Multiscale information about the dynamic behavior of miRNA and the probe is acquired. Next, via SERS reverse signal ratio response and a novel enzyme-free numerator signal amplification, the amplified signal and reduced black value were achieved in the quantification of miRNA. More importantly, BSR probes showed good stability in cells and were successfully used for accurate tracing and quantification of miR-203 from MCF-7 cells. Therefore, the reported BSR probe is a potential tool for the reliable monitoring of biomolecule dynamics in living cells.
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http://dx.doi.org/10.1021/acs.analchem.0c03491DOI Listing
January 2021

Coagulation dysfunction in ICU patients with coronavirus disease 2019 in Wuhan, China: a retrospective observational study of 75 fatal cases.

Aging (Albany NY) 2020 12 9;13(2):1591-1607. Epub 2020 Dec 9.

Department of Critical Care Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, PR China.

Coagulation dysfunction in critically ill patients with coronavirus disease 2019 (COVID-19) has not been well described, and the efficacy of anticoagulant therapy is unclear. In this study, we retrospectively reviewed 75 fatal COVID-19 cases who were admitted to the intensive care unit at Jinyintan Hospital (Wuhan, China). The median age of the cases was 67 (62-74) years, and 47 (62.7%) were male. Fifty patients (66.7%) were diagnosed with disseminated intra-vascular coagulation. Approximately 90% of patients had elevated D-dimer and fibrinogen degradation products, which decreased continuously after anticoagulant treatment and was accompanied by elevated albumin (all <0.05). The median survival time of patients treated with anticoagulant was 9.0 (6.0-14.0) days compared with 7.0 (3.0-10.0) days in patients without anticoagulant therapy (=0.008). After anticoagulation treatment, C-reactive protein levels decreased (=0.004), as did high-sensitivity troponin (=0.018), lactate dehydrogenase (<0.001), and hydroxybutyrate dehydrogenase (<0.001). In conclusion, coagulation disorders were widespread among fatal COVID-19 cases. Anticoagulant treatment partially improved hypercoagulability, prolonged median survival time, and may have postponed inflammatory processes and cardiac injury.
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http://dx.doi.org/10.18632/aging.202223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880373PMC
December 2020

Obesity is associated with increased severity of disease in COVID-19 pneumonia: a systematic review and meta-analysis.

Eur J Med Res 2020 Dec 2;25(1):64. Epub 2020 Dec 2.

Department of Cardiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, No. 79 Qingchun Road, Hangzhou, 310006, Zhejiang, China.

Background: Obesity has been widely reported to be associated with the disease progression of coronavirus disease 2019 (COVID-19); however, some studies have reported different findings. We conducted a systematic review and meta-analysis to investigate the association between obesity and poor outcomes in patients with COVID-19 pneumonia.

Methods: A systematic review and meta-analysis of studies from the PubMed, Embase, and Web of Science databases from 1 November 2019 to 24 May 2020 was performed. Study quality was assessed, and data extraction was conducted. The meta-analysis was carried out using fixed-effects and random-effects models to calculate odds ratios (ORs) of several poor outcomes in obese and non-obese COVID-19 patients.

Results: Twenty-two studies (n = 12,591 patients) were included. Pooled analysis demonstrated that body mass index (BMI) was higher in severe/critical COVID-19 patients than in mild COVID-19 patients (MD 2.48 kg/m, 95% CI [2.00 to 2.96 kg/m]). Additionally, obesity in COVID-19 patients was associated with poor outcomes (OR = 1.683, 95% CI [1.408-2.011]), which comprised severe COVID-19, ICU care, invasive mechanical ventilation use, and disease progression (OR = 4.17, 95% CI [2.32-7.48]; OR = 1.57, 95% CI [1.18-2.09]; OR = 2.13, 95% CI [1.10-4.14]; OR = 1.41, 95% CI [1.26-1.58], respectively). Obesity as a risk factor was greater in younger patients (OR 3.30 vs. 1.72). However, obesity did not increase the risk of hospital mortality (OR = 0.89, 95% CI [0.32-2.51]).

Conclusions: As a result of a potentially critical role of obesity in determining the severity of COVID-19, it is important to collect anthropometric information for COVID-19 patients, especially the younger group. However, obesity may not be associated with hospital mortality, and efforts to understand the impact of obesity on the mortality of COVID-19 patients should be a research priority in the future.
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http://dx.doi.org/10.1186/s40001-020-00464-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708895PMC
December 2020

Downregulation of Cypher induces apoptosis in cardiomyocytes via Akt/p38 MAPK signaling pathway.

Int J Med Sci 2020 27;17(15):2328-2337. Epub 2020 Aug 27.

Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Dilated cardiomyopathy (DCM) is considered as the most common form of non-ischemic cardiomyopathy with a high mortality worldwide. Cytoskeleton protein Cypher plays an important role in maintaining cardiac function. Genetic studies in human and animal models revealed that Cypher is involved in the development of DCM. However, the underlying molecular mechanism is not fully understood. Accumulating evidences suggest that apoptosis in myocytes may contribute to DCM. Thus, the purpose of this study is to define whether lack of Cypher in cardiomyocytes can elevate apoptosis signaling and lead to DCM eventually. Cypher-siRNA sufficiently inhibited Cypher expression in cardiomyocytes. TUNEL-positive cardiomyocytes were increased in both Cypher knockdown neonatal rat cardiomyocytes and Cypher knockout mice hearts, which were rare in the control group. Flow cytometry further confirmed that downregulation of Cypher significantly increased myocytes apoptosis . Cell counting kit-8 assay revealed that Cypher knockdown in H9c2 cells significantly reduced cell viability. Cypher knockdown was found to increase cleaved caspase-3 expression and suppress p21, ratio of bcl-2 to Bax. Cypher-deficiency induced apoptosis was linked to downregulation of Akt activation and elevated p-p38 MAPK accumulation. Pharmacological activation of Akt with SC79 attenuated apoptosis with enhanced phosphorylation of Akt and reduced p-p38 MAPK and Bax expression. Downregulation of Cypher participates in the promotion of cardiomyocytes apoptosis through inhibiting Akt dependent pathway and enhancing p38 MAPK phosphorylation. These findings may provide a new potential therapeutic strategy for the treatment of DCM.
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http://dx.doi.org/10.7150/ijms.48872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7484636PMC
August 2020

A simple method for the synthesis of biochar nanodots using hydrothermal reactor.

MethodsX 2020 12;7:101022. Epub 2020 Aug 12.

Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, United States.

Biochar is a stable carbon rich by-product synthesized through pyrolysis of plant and animal based biomass, and nano-biochar material has gained increasing attention due to its unique properties for environmental applications. In the present study, a simple cost-effective method for the synthesis of biochar nanoparticles through hydrothermally using agricultural residuals and by-products was developed. Both soybean straw and cattle manure were selected as the feedstock to produce the bulk-biochar. The synthesis procedure involved the digestion of the bulk-biochar with concentrated nitric acid and sulfuric acid in a high pressure condition using a hydrothermal reactor. The suspension was isolated using vacuum filtration with 0.22-μm membrane followed by drying at 65 °C in an oven. Scanning electron microscopy results revealed that both of the biochars had a well-developed porous structure following pyrolysis. Both transmission electron microscopy and the dynamic light scattering results of the hydrothermally treated biochar indicated that the soybean straw and cattle manure biochar nanodots had an average of 5-nm and 4-nm in size, respectively. Overall two raw materials produced 8.5-10% biochar nanodots. The present method presents a simple, quick and cost-effective method for synthesis of biochar nanodots. The method provided a useful tool discovering the applicability biochar nanodots for environmental applications. • Nano-biochar formation from bulk-biochar using hydrothermal reactor • Evaluate nano-biochar's environmental fate and behavior in soil and water • Synthesize multifunctional adsorbent using nano-biochar as primary material.
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http://dx.doi.org/10.1016/j.mex.2020.101022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7452209PMC
August 2020

Determinants of left atrial thrombus or spontaneous echo contrast in nonvalvular atrial fibrillation.

Thromb Res 2020 11 7;195:233-237. Epub 2020 Aug 7.

Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. Electronic address:

Background: The CHADS and CHADS-VASc scores are well-established clinical scales to estimate the risk of stroke in patients with atrial fibrillation (AF). However, the predictive power of the two scales concerning left atrial thrombus (LAT) or spontaneous echo contrast (SEC) has not been well investigated. Therefore, we investigated the predict power of CHADS and CHADS-VASc scores concerning LAT/SEC; identified clinical, echocardiographic and laboratory predictors of LAT/SEC in addition to the CHADS and CHADS-VASc scores; and derived a new scale to predict LAT/SEC accurately, it might improve thromboembolic risk stratification in patients with nonvalvular atrial fibrillation.

Methods: We identified 1102 consecutive AF patients who underwent transesophageal echocardiography (TEE) for the purpose of the exclusion of LAT before catheter ablation, cardioversion or left atrial appendage occlusion. The clinical, echocardiographic and laboratory characteristics of patients were collected from the electronic medical record system.

Results: In the study, the prevalence of LAT/SEC was only 4.36%. In the multivariate logistic analysis, hypertension, left atrial enlargement, prior stroke/TIA, left ventricular dysfunction, and renal dysfunction were predictors of LAT/SEC. Receiver operating characteristic curve analysis showed that c-statistics of the CHADS and CHADS-VASc scores concerning LAT/SEC were 0.673 and 0.643, respectively. We derived a new scale composed of variables from the multivariate logistic analysis that showed a higher c-statistic value (0.761) than the CHADS and CHADS-VASc scores for the prediction of LAT/SEC.

Conclusion: In our cohort, we found two variables not included in the CHADS-VASc score (renal dysfunction, left atrial enlargement) were independent predictors of LAT/SEC. A new scale combining clinical, echocardiographic and laboratory predictors might improve thromboembolic risk stratification. And there is a great need to carry out a new prospective and multicenter study, with a population more homogenous and including all the determinants for LAT/SEC to establish the independent degree of each variable and the applicability in clinical practice, facilitating the emergence of a new score of thromboembolic risk in patients with nonvalvular atrial fibrillation.
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http://dx.doi.org/10.1016/j.thromres.2020.07.055DOI Listing
November 2020

A new perspective on the toxicity of arsenic-contaminated soil: Tandem mass tag proteomics and metabolomics in earthworms.

J Hazard Mater 2020 11 20;398:122825. Epub 2020 May 20.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China; University of the Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

The toxicity of low-level arsenic (As)-contaminated soil is not well understood. An integrated proteomic and metabolomic approach combined with morphological examination was used to investigate the potential biological toxicity of As-contaminated soil based on an exposure experiment with the earthworm Eisenia fetida. The results showed that the earthworm hindgut accumulated high As concentrations resulting in injury to the intestinal epithelia, chloragogenous tissues and coelom tissues. Furthermore, As-contaminated soil induced a significant increase in betaine levels and a decrease in dimethylglycine and myo-inositol levels in the earthworms, suggesting that the osmoregulatory metabolism of the earthworms may have been disturbed. The significantly altered levels of asparagine and dimethylglycine were proposed as potential biomarkers of As-contaminated soil. The upregulation of soluble calcium-binding proteins and profilin, the downregulation of sodium/potassium-transporting ATPase, and the proteins changes identified by gene ontology enrichment analysis confirmed that the earthworms suffered from osmotic stress. In addition, the significant changes in glycine-tRNA ligase activity and coelomic tissue injury revealed that As accumulation may disturb the earthworm immune system. This work provided new insight into the proteomic and metabolic toxicity of low-level As-contaminated soil ecosystems in earthworms, extended our knowledge of dual omics and highlighted the mechanisms underlying toxicity.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122825DOI Listing
November 2020

Prognostic Value of Cardiac Magnetic Resonance-Derived Right Ventricular Remodeling Parameters in Pulmonary Hypertension: A Systematic Review and Meta-Analysis.

Circ Cardiovasc Imaging 2020 07 16;13(7):e010568. Epub 2020 Jul 16.

Department of Cardiology, The First Affiliated Hospital of Zhejiang University School of Medicine (Y.D., Z.P., D.W., J.L., J.F., R.X., J.D., X.C., X.X., X.W., X.G.).

Background Cardiac right ventricular remodeling plays a substantial role in pathogenesis, progression, and prognosis of pulmonary hypertension. Cardiac magnetic resonance is considered an excellent tool for evaluation of right ventricle. However, value of right ventricular remodeling parameters derived from cardiac magnetic resonance in predicting adverse events is controversial. Methods The Pubmed (MEDLINE), Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure platform (CNKI), China Science and Technology Journal Database (VIP), and Wanfang databases were systematically searched until November 2019. Studies reporting hazard ratios (HRs) for all-cause death and composite end point of pulmonary hypertension were included. Univariate HRs were extracted from the included studies to calculate pooled HRs of each right ventricular remodeling parameter. Results Eight studies with 1120 patients examining all-cause death (female: 44%-92%, age: 40-67 years old, follow-up time: 27-48 months) and 10 studies with 604 patients examining composite end point (female: 60%-83%, age: 29-57 years old, follow-up time: 10-68 months) met the criteria. Right ventricular ejection fraction was the only parameter which could predict both all-cause death (pooled HR=0.95; =0.014) and composite end point (pooled HR=0.95; <0.001), although right ventricular end-diastolic volume index (pooled HR=1.01; <0.001), right ventricular end-systolic volume index (pooled HR=1.01, =0.045), and right ventricular mass index (pooled HR=1.03, =0.032) only predicted composite outcome. Similar results were observed when we conducted the meta-analysis among patients with World Health Organization type I of pulmonary hypertension. Conclusions Cardiac magnetic resonance-derived right ventricular remodeling parameters have independent prognostic value for all-cause death and composite end point of patients with pulmonary hypertension. Right ventricular ejection fraction was the strongest prognostic factor among all the right ventricular remodeling parameters. Right ventricular mass index, right ventricular end-diastolic volume index, and right ventricular end-systolic volume index also demonstrated prognostic value.
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http://dx.doi.org/10.1161/CIRCIMAGING.120.010568DOI Listing
July 2020

Multiple Non-coding ANRIL Transcripts Are Associated with Risk of Coronary Artery Disease: a Promising Circulating Biomarker.

J Cardiovasc Transl Res 2021 Apr 22;14(2):229-237. Epub 2020 Jun 22.

Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.

Multiple ANRIL transcriptional isoforms, such as lncANRIL and circANRIL have been identified. We sought to explore their diagnostic value in patients with coronary artery disease (CAD). First, we selected six target ANRIL isoforms and measured their expression in CAD patients and controls in the peripheral blood. Their diagnostic values were evaluated. circANRIL(exon14-4) was identified as the best potential biomarker. Afterwards, we validated its diagnostic value and evaluated its prognostic value in a larger clinical cohort. Among six tested ANRILs, lncANRIL(exon1) and lncANRIL(exon4-6) in the CAD patients were significantly increased, while circANRIL(exon14-4) was downregulated. circANRIL(exon14-4) had the highest diagnostic value among the three isoforms. The combination of circANRIL(exon14-4) and other factors resulted in a more accurate differentiation of CAD patients. Moreover, lower expression of circANRIL(exon14-4) was associated with higher incidence of MACE. circANRIL(exon14-4) is closely associated with CAD risk and severity, which provides a promising circulating biomarker for CAD diagnosis and prognosis.
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http://dx.doi.org/10.1007/s12265-020-10053-0DOI Listing
April 2021

SARS-CoV-2 was found in the bile juice from a patient with severe COVID-19.

J Med Virol 2021 Jan 29;93(1):102-104. Epub 2020 Jun 29.

Department of Cardiology, The First Affiliated Hospital of Zhejiang University, Hangzhou, China.

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http://dx.doi.org/10.1002/jmv.26169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307073PMC
January 2021

Selenium enhances iron plaque formation by elevating the radial oxygen loss of roots to reduce cadmium accumulation in rice (Oryza sativa L.).

J Hazard Mater 2020 11 17;398:122860. Epub 2020 May 17.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; Ecological Experimental Station of Red Soil, Chinese Academy of Sciences, Yingtan 335211, China. Electronic address:

The inhibition of cadmium (Cd) absorption by selenium (Se) in rice may be associated with iron plaque (IP) formation, but the driving mechanisms are still unclear. This study investigated the effects of Se on the growth, oxidative toxicity, radial oxygen loss (ROL), IP formation, and Cd absorption of rice exposed to Cd. The results of this study showed that Cd stress elevated the levels of O- and HO and depressed superoxide dismutase (SOD) and catalase (CAT) activities. The maximum ROL and IP were reduced by 43.3 % and 74.5 %, respectively. However, Se alleviated Cd toxicity by stimulating SOD and CAT activities by scavenging O- and HO and enhancing the ROL profiles. Under culture conditions without Fe, Se had no impact on the total Cd levels in rice (T). However, with the addition of Fe, T was significantly reduced by 23.3 % due to the enhancement of IP formation by Se. These results indicated that Se can reduce Cd accumulation in rice in the presence of Fe treatments. However, Se just alleviated Cd toxicity in the absence of Fe treatments. The enhancement of ROL was a potential reason for the elevated IP formation induced by Se.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122860DOI Listing
November 2020

Clinical features of critically ill patients with confirmed COVID-19.

J Infect 2020 07 28;81(1):147-178. Epub 2020 Apr 28.

Department of Cardiology, The First Affiliated Hospital of Zhejiang University, Hangzhou, China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2020.03.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194653PMC
July 2020

Assessment of the immobilization effectiveness of several amendments on a cadmium-contaminated soil using Eisenia fetida.

Ecotoxicol Environ Saf 2020 Feb 20;189:109948. Epub 2019 Nov 20.

CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China; Ecological Experimental Station of Red Soil, Chinese Academy of Sciences, Yingtan, 335211, China. Electronic address:

Proper protocols for assessing the remediation effectiveness of contaminated soils are an important part of remediation projects. In the present study, the residual immobilization effectiveness of hydrated lime (L), hydroxyapatite (H), biochar (B) and organic fertilizer (F) alone and in combination was assessed by Eisenia fetida. The results showed that the application of amendments had no significant effect on the death rate and average fresh weight loss of earthworms. The earthworm Cd concentration increased with prolonged exposure time, however, the significant immobilization efficacy of amendments observed on the 7th day nearly disappeared after 28 days of exposure. The immobilization efficiencies, estimated by the earthworms internal Cd concentration, of L, H and B on the 7th day were 38.6%, 37.8% and 20.7%, respectively. These values decreased to 4.9%, 19.8% and 15.1%, respectively, on the 28th day. The detoxification effect of amendments was confirmed by the Cd subcellular fractionation in earthworms with lower proportions of Cd distributed in the metal-sensitive fractions in L, H and B treatments. The level of oxidative stress response of earthworms increased with exposure duration and amendments alleviated the oxidative damage induced by Cd to the earthworms. In addition, the pH and CaCl-Cd in soils were both increased due to earthworm life activities and gut-related ingestion. In summary, the assessment of immobilization effectiveness of heavy metal-contaminated soils using Eisenia fetida was time-dependent. The immobilization efficacy of L and H performed better than B and F on the 7th day, while H and B performed better than L and F on the 28th day. Accordingly, the short-term earthworm exposure experiment (7 days) was recommended to be an alternative approach to time-consuming plant bioassays in assessment of reduced phytoavailability in chemical immobilization remediation. But the impact of earthworms on the immobilization effect of amendments needs to be considered in practical remediation.
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http://dx.doi.org/10.1016/j.ecoenv.2019.109948DOI Listing
February 2020

Volatile-mediated antagonism of soil bacterial communities against fungi.

Environ Microbiol 2020 03 4;22(3):1025-1035. Epub 2019 Nov 4.

Department of Microbial Ecology, Netherlands Institute of Ecology, NIOO-KNAW, Wageningen, 6708 PB, The Netherlands.

Competition is a major type of interaction between fungi and bacteria in soil and is also an important factor in suppression of plant diseases caused by soil-borne fungal pathogens. There is increasing attention for the possible role of volatiles in competitive interactions between bacteria and fungi. However, knowledge on the actual role of bacterial volatiles in interactions with fungi within soil microbial communities is lacking. Here, we examined colonization of sterile agricultural soils by fungi and bacteria from non-sterile soil inoculums during exposure to volatiles emitted by soil-derived bacterial communities. We found that colonization of soil by fungi was negatively affected by exposure to volatiles emitted by bacterial communities whereas that of bacteria was barely changed. Furthermore, there were strong effects of bacterial community volatiles on the assembly of fungal soil colonizers. Identification of volatile composition produced by bacterial communities revealed several compounds with known fungistatic activity. Our results are the first to reveal a collective volatile-mediated antagonism of soil bacteria against fungi. Given the better exploration abilities of filamentous fungi in unsaturated soils, this may be an important strategy for bacteria to defend occupied nutrient patches against invading fungi. Another implication of our research is that bacterial volatiles in soil atmospheres can have a major contribution to soil fungistasis.
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http://dx.doi.org/10.1111/1462-2920.14808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064993PMC
March 2020

Kinetic characteristics and predictive models of methylmercury production in paddy soils.

Environ Pollut 2019 Oct 10;253:424-428. Epub 2019 Jul 10.

CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address:

Understanding the mercury (Hg) methylation process is important for the management of paddy soils contaminated by Hg. In this work, samples of eighteen paddy soils with varying soil properties were spiked with inorganic Hg and subjected to a 90 d flooding period. Soil pH and redox potential (Eh) were measured in situ at intervals, and soils were sampled for the analysis of methylmercury (MeHg). The Hg methylation efficiency increased with flooding time and reached a relatively steady state at 30 d of incubation, ranging from 0.08% to 2.52%, and was significantly correlated with the in situ soil pH and Eh. The Elovich equation could adequately describe the kinetic production of MeHg. MeHg production was well predicted by the in situ soil pH and Eh of flooded soils, in addition to the organic matter content of air-dried soil samples and flooding time. The two predictive models explained 78% and 68% of the variability of the Hg methylation efficiency. The results suggested that the methylation of inorganic Hg in paddy soils after flooding can be predicted as a function of routinely measured soil properties and flooding time, a correlation that can be utilized to improve understanding of the extent of Hg methylation and the management of Hg-contaminated paddy soils.
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http://dx.doi.org/10.1016/j.envpol.2019.07.050DOI Listing
October 2019

Toxic responses of metabolites, organelles and gut microorganisms of Eisenia fetida in a soil with chromium contamination.

Environ Pollut 2019 Aug 16;251:910-920. Epub 2019 May 16.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. Electronic address:

The toxic sensitivity in different physiological levels of chromium (Cr) contaminated soils with environmentally equivalent concentrations (EEC) was fully unknown. The earthworm Eisenia fetida was exposed to a Cr-contaminated soil at the EEC level (referred to as Cr-CS) to characterize the induced toxicity at the whole body, organ, tissue, subcellular structure and metabolic levels. The results showed that the survival rate, weight and biodiversity of the gut microorganisms (organ) had no significant difference (p > 0.05) between control and Cr-CS groups. Qualitative histopathological and subcellular evaluations from morphology showed earthworms obvious injuries. The organelle injuries combined with the metabolic changes provided additional evidence that the Cr-CS damaged the nucleus and probably disturbed the nucleic acid metabolism of earthworms. 2-hexyl-5-ethyl-3-furansulfonate, dimethylglycine, betaine and scyllo-inositol were sensitive and relatively quantitative metabolites that were recommended as potential biomarkers for Cr-CS based on their significant weights in the multivariate analysis model. In addition, the relative abundance of Burkholderiaceae, Enterobacteriaceae and Microscillaceae of the earthworm guts in the Cr-CS group significantly increased, particularly for Burkholderiaceae (increased by 13.1%), while that of Aeromonadaceae significantly decreased by 5.6% in contrast with the control group. These results provided new insights into our understanding of the toxic effects of the EEC level of Cr contaminated soil from different physiological levels of earthworms and extend our knowledge on the composition and sensitivity of the earthworm gut microbiota in Cr contaminated soil ecosystems. Furthermore, these toxic responses from gut microorganisms to metabolites of earthworms provided important data to improve the adverse outcome pathway and toxic mechanism of the Cr-CS if the earthworm genomics and proteomics would be also gained in the future.
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http://dx.doi.org/10.1016/j.envpol.2019.05.069DOI Listing
August 2019

Anomalous origin of the left coronary artery from the pulmonary artery in a patient of advanced age: a case report and analysis.

J Int Med Res 2019 Jun 14;47(6):2687-2693. Epub 2019 Apr 14.

1 Cardiology Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

A 61-year-old Chinese man presented with a nearly 30-year history of an anomalous origin of the left coronary artery. He had been diagnosed with an anomalous origin of the left coronary artery in 1989. He then underwent regular echocardiographic examinations and it was found that his heart was gradually enlarging. After a >20-year asymptomatic period, he developed recurrent chest discomfort and palpitation. Coronary computed tomography angiography suggested that the left coronary artery anomaly originated from the pulmonary artery; additionally, the right coronary artery was tortuous and thickened. Coronary angiography showed that the right coronary artery was huge and buckling. The patient underwent corrective surgery of the anomalous origin of the left coronary artery from the pulmonary artery, aortic valve mechanical valve replacement, mitral valve plasty, and tricuspid valve plasty in Fuwai Hospital (National Center of Cardiovascular Disease of China), and the anatomic results of the surgery were good.
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http://dx.doi.org/10.1177/0300060519841509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567725PMC
June 2019

Influence of bacterial community composition and soil factors on the fate of phenanthrene and benzo[a]pyrene in three contrasting farmland soils.

Environ Pollut 2019 Apr 8;247:229-237. Epub 2019 Jan 8.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

The fate of polycyclic aromatic hydrocarbons (PAHs) determines their potential risk in soil, which may be directly affected by abiotic conditions and indirectly through the changes in decomposer communities. In comparison, the indirect effects on the fate remain largely elusive. In this study, the fate of phenanthrene and benzo[a]pyrene and the corresponding bacterial changes were investigated in three contaminated farmland soils using a C tracer method and Miseq sequencing. The results showed that most benzo[a]pyrene was consistently extractable with dichloromethane (DCM) after the 60-day incubation (60.4%-78.2%), while phenanthrene was mainly mineralized to CO during the 30-day incubation (40.4%-58.7%). Soils from Guangzhou (GZ) showed a different distribution pattern of C-PAHs exemplified by low mineralization and disparate bound residue formation. The PAH fate in the Shenyang (SY) and Nanjing (NJ) soils were similar to each other than to that in the GZ soil. The fate in the GZ soil seemed to be linked to the distinct edaphic properties, such as organic matter content, however soil microbial community could have influenced the distribution pattern of PAHs. This potential role of microorganisms was reflected by the unique changes in the copy numbers of Gram positive RHD gene, and by the distinct shifts in bacterial community composition during the incubation. A quite different shift in bacterial communities was found in the GZ microcosms which may influence PAH mineralization and non-extractable residue (NER) formation.
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http://dx.doi.org/10.1016/j.envpol.2018.12.079DOI Listing
April 2019

Characteristics of Time-Dependent Selenium Biofortification of Rice ( Oryza sativa L.).

J Agric Food Chem 2018 Nov 15;66(47):12490-12497. Epub 2018 Nov 15.

CAS Key Laboratory of Soil Environment and Pollution Remediation , Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008 , China.

The application of selenite to soil has increasingly been used to produce Se-enriched food. This study investigated the biofortification characteristics of Se in rice after application of selenite to soil at different growth stages. The results showed that the application of Se during booting stage resulted in the highest concentration of Se in brown rice due to the highest upward translocation of Se. More than 90% of Se in the brown rice was organic species, with selenomethionine predominated. The proportion of selenomethionine in the brown rice decreased with the delay in application time. The rice grown in the acidic soil had higher Se concentrations than in the neutral soil. With increasing soil Cd level, Se accumulation and the proportion of Se-methylselenocysteine in the brown rice were reduced. This study provides a theoretical basis for the production of Se-enriched rice in clean soil or slightly to moderately Cd-contaminated soil.
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http://dx.doi.org/10.1021/acs.jafc.8b04502DOI Listing
November 2018

Legacy of land use history determines reprogramming of plant physiology by soil microbiome.

ISME J 2019 03 27;13(3):738-751. Epub 2018 Oct 27.

Department of Microbial Ecology, Netherlands Institute of Ecology, NIOO-KNAW, Wageningen, 6708 PB, The Netherlands.

Microorganisms associated with roots are thought to be part of the so-called extended plant phenotypes with roles in the acquisition of nutrients, production of growth hormones, and defense against diseases. Since the crops selectively enrich most rhizosphere microbes out of the bulk soil, we hypothesized that changes in the composition of bulk soil communities caused by agricultural management affect the extended plant phenotype. In the current study, we performed shotgun metagenome sequencing of the rhizosphere microbiome of the peanut (Arachis hypogaea) and metatranscriptome analysis of the roots of peanut plants grown in the soil with different management histories, peanut monocropping and crop rotation. We found that the past planting record had a significant effect on the assembly of the microbial community in the peanut rhizosphere, indicating a soil memory effect. Monocropping resulted in a reduction of the rhizosphere microbial diversity, an enrichment of several rare species, and a reduced representation of traits related to plant performance, such as nutrients metabolism and phytohormone biosynthesis. Furthermore, peanut plants in monocropped soil exhibited a significant reduction in growth coinciding with a down-regulation of genes related to hormone production, mainly auxin and cytokinin, and up-regulation of genes related to the abscisic acid, salicylic acid, jasmonic acid, and ethylene pathways. These findings suggest that land use history affects crop rhizosphere microbiomes and plant physiology.
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http://dx.doi.org/10.1038/s41396-018-0300-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461838PMC
March 2019

A tillering application of zinc fertilizer based on basal stabilization reduces Cd accumulation in rice (Oryza sativa L.).

Ecotoxicol Environ Saf 2019 Jan 22;167:338-344. Epub 2018 Oct 22.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; Ecological Experimental Station of Red Soil, Chinese Academy of Sciences, Yingtan 335211, China. Electronic address:

Cadmium (Cd) contamination in paddy fields has received extensive attention throughout the world, especially in China. In this study, treatments of a lime application with or without zinc sulfate as basal fertilizer, a basal or tillering application of zinc sulfate, and basal stabilization using lime combined with a tillering application of zinc sulfate were designed in a field trial to investigate their contributions to the uptake and translocation of Cd in rice plants. The results showed that basal stabilization using lime significantly decreased brown rice Cd by 42%; the CaCl-extractable Cd in the soil was decreased by 46-51%, but the phytoavailability of Zn in the soil was also inhibited. The basal or tillering application of zinc sulfate significantly inhibited the upward transport of Cd (from the root to the shoot) while having no significant impact on CaCl-extractable Cd; consequently, the concentration of Cd in the brown rice was reduced by only 17-25%. Compared with the lime application alone, the basal application of lime together with zinc sulfate did not further reduce the Cd in brown rice. However, basal stabilization using lime combined with the tillering application of zinc decreased the Cd in brown rice by 73%, which was attributed to the reduced CaCl-extractable Cd and the competitive effect of Zn on Cd, in which the inhibition of the upward transport of Cd inside the root played an important role. Two field verification tests conducted during the next year also demonstrated that this combined method significantly decreased the level of Cd in brown rice.
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http://dx.doi.org/10.1016/j.ecoenv.2018.10.044DOI Listing
January 2019

Topdressing iron fertilizer coupled with pre-immobilization in acidic paddy fields reduced cadmium uptake by rice (Oryza sativa L.).

Sci Total Environ 2018 Sep 3;636:1040-1047. Epub 2018 May 3.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; Ecological Experimental Station of Red Soil, Chinese Academy of Sciences, Yingtan 335211, China. Electronic address:

Soil cadmium (Cd) contamination has become a serious problem in China. This study was conducted to test the effects of basal application of hydrated lime and iron fertilizer alone or together and topdressing of iron fertilizer at the tillering stage alone or coupled with basal application of hydrated lime, on reducing the accumulation of Cd in brown rice grown in an acidic paddy field slightly contaminated with Cd. The results showed that Cd in brown rice (BR-Cd) was dependent on not only the pH increase and CaCl-extractable Cd reduction in the soil due to lime amendment but also Cd sequestration by the iron plaque on root surfaces. However, lime significantly decreased the amounts of Fe and Cd in the iron plaque on the surface of rice root. Topdressing of ferrous sulfate at the tillering stage resulted in the highest Fe and Cd sequestration in the iron plaque. Compared with the control (0.71 mg kg BR-Cd), the basal application of lime and ferrous sulfate alone or together reduced BR-Cd by 45.8%, 18.3%, and 53.1%, respectively; topdressing of ferrous sulfate alone reduced BR-Cd by 23.6%, and topdressing of ferrous sulfate at the tillering stage coupled with basal application of lime yielded the lowest BR-Cd level with a 74.6% reduction. This result was further confirmed by field experiments at two sites in the following year.
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http://dx.doi.org/10.1016/j.scitotenv.2018.04.369DOI Listing
September 2018

Stability of immobilization remediation of several amendments on cadmium contaminated soils as affected by simulated soil acidification.

Ecotoxicol Environ Saf 2018 10 4;161:164-172. Epub 2018 Jun 4.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 71 East Beijing Road, Nanjing 210008, China; Ecological Experimental Station of Red Soil, Chinese Academy of Sciences, Yingtan 335211, China. Electronic address:

Chemical immobilization is a practical approach to remediate heavy metal contamination in agricultural soils. However, the potential remobilization risks of immobilized metals are a major environmental concern, especially in acid rain zones. In the present study, changes in the immobilization efficiency of several amendments as affected by simulated soil acidification were investigated to evaluate the immobilization remediation stability of several amendments on two cadmium (Cd) contaminated soils. Amendments (hydrated lime, hydroxyapatite and biochar) effectively immobilized Cd, except for organic fertilizer, and their immobilizations were strongly decreased by the simulated soil acidification. The ratio of changes in CaCl-extractable Cd: pH (△CaCl-Cd/△pH) can represent the Cd remobilization risk of different amended soils. Hydroxyapatite and biochar had a stronger durable immobilizing effect than did hydrated lime, particularly in soil with a lower pH buffering capacity, which was further confirmed by the Cd concentration and accumulation in lettuce. These results can be attributed to that hydroxyapatite and biochar transformed greater proportions of exchangeable Cd to other more stable fractions than lime. After 48 weeks of incubation, in soil with a lower pH buffering capacity, the immobilization efficiencies of lime, hydroxyapatite, biochar and organic fertilizer in the deionized water group (pH 6.5) were 71.7%, 52.7%, 38.6% and 23.9%, respectively, and changed to 19.1%, 33.6%, 26.5% and 5.0%, respectively, in the simulated acid rain group (pH 2.5). The present study provides a simple method to preliminarily estimate the immobilization efficiency of amendments and predict their stability in acid rain regions before large-scale field application. In addition, hydrated lime is recommended to be combined with other acid-stable amendments (such as hydroxyapatite or biochar) to remediate heavy metal-contaminated agricultural soils in acid precipitation zones.
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http://dx.doi.org/10.1016/j.ecoenv.2018.05.088DOI Listing
October 2018

Main controlling factors and forecasting models of lead accumulation in earthworms based on low-level lead-contaminated soils.

Environ Sci Pollut Res Int 2018 Aug 2;25(23):23117-23124. Epub 2018 Jun 2.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, People's Republic of China.

To explore the main controlling factors in soil and build a predictive model between the lead concentrations in earthworms (Pb) and the soil physicochemical parameters, 13 soils with low level of lead contamination were used to conduct toxicity experiments using earthworms. The results indicated that a relatively high bioaccumulation factor appeared in the soils with low pH values. The lead concentrations between earthworms and soils after log transformation had a significantly positive correlation (R = 0.46, P < 0.0001, n = 39). Stepwise multiple linear regression analysis derived a fitting empirical model between Pb and the soil physicochemical properties: log(Pb) = 0.96log(Pb) - 0.74log(OC) - 0.22pH + 0.95, (R = 0.66, n = 39). Furthermore, path analysis confirmed that the Pb concentrations in the soil (Pb), soil pH, and soil organic carbon (OC) were the primary controlling factors of Pb with high pathway parameters (0.71, - 0.51, and - 0.49, respectively). The predictive model based on Pb in a nationwide range of soils with low-level lead contamination could provide a reference for the establishment of safety thresholds in Pb-contaminated soils from the perspective of soil-animal systems.
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http://dx.doi.org/10.1007/s11356-018-2436-xDOI Listing
August 2018

NMR-based metabolic toxicity of low-level Hg exposure to earthworms.

Environ Pollut 2018 Aug 19;239:428-437. Epub 2018 Apr 19.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, People's Republic of China. Electronic address:

Mercury is a globally distributed toxicant to aquatic animals and mammals. However, the potential risks of environmental relevant mercury in terrestrial systems remain largely unclear. The metabolic profiles of the earthworm Eisenia fetida after exposure to soil contaminated with mercury at 0.77 ± 0.09 mg/kg for 2 weeks were investigated using a two-dimensional nuclear magnetic resonance-based (H-C NMR) metabolomics approach. The results revealed that traditional endpoints (e.g., mortality and weight loss) did not differ significantly after exposure. Although histological examination showed sub-lethal toxicity in the intestine as a result of soil ingestion, the underlying mechanisms were unclear. Metabolite profiles revealed significant decreases in glutamine and 2-hexyl-5-ethyl-3-furansulfonate in the exposed group and remarkable increases in glycine, alanine, glutamate, scyllo-inositol, t-methylhistidine and myo-inositol. More importantly, metabolic network analysis revealed that low mercury in the soil disrupted osmoregulation, amino acid and energy metabolisms in earthworms. A metabolic net link and schematic diagram of mercury-induced responses were proposed to predict earthworm responses after exposure to mercury at environmental relevant concentrations. These results improved the current understanding of the potential toxicity of low mercury in terrestrial systems.
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http://dx.doi.org/10.1016/j.envpol.2018.04.027DOI Listing
August 2018

Endovascular coil embolization and stenting for the treatment of iatrogenic right internal mammary artery injury: A case report.

J Int Med Res 2018 Mar 11;46(3):1271-1276. Epub 2018 Jan 11.

1 Department of Cardiology, First Affiliated Hospital, 26441 School of Medicine, Zhejiang University , Hangzhou, Zhejiang Province, China.

A 54-year-old Chinese woman presented with a 10-year history of repeated paroxysmal palpitations. She was diagnosed with paroxysmal supraventricular tachycardia by 12-lead electrocardiogram and was advised to undergo catheter-based radiofrequency ablation. During the procedure, a rare complication occurred that was diagnosed as a right internal mammary artery penetrating injury. After appropriate emergency treatment with arterial embolization and membrane-covered stent implantation, the patient was out of immediate danger of haemorrhaging. Follow-up computed tomography angiography of the subclavian artery at 3 months after she was discharged from hospital revealed stent-graft patency with no evidence of in-stent thrombosis or stent stenosis. No problems were observed at the 6-month follow-up visit.
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http://dx.doi.org/10.1177/0300060517740307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972256PMC
March 2018