Publications by authors named "Xingmin Wang"

71 Publications

Extracellular superoxide produced by Enterococcus faecalis reduces endometrial receptivity via inflammatory injury.

Am J Reprod Immunol 2021 May 15:e13453. Epub 2021 May 15.

Nantong Institute of Genetics and Reproductive Medicine, Affiliated Maternity and Child Healthcare Hospital of Nantong University, Nantong, Jiangsu, China.

Problem: Chronic endometritis (CE) can cause infertility. Enterococcus faecalis is an opportunistic pathogen that is often found in the endometrium of CE patients. However, the mechanisms by which E. faecalis affects endometrial health are still unclear. In this study, we investigated the mechanism how extracellular superoxide produced by E. faecalis affected the endometrial receptivity.

Method Of Study: Superoxide production was blocked by deleting menB gene in E. faecalis OG1RF. Endometrial epithelial cells were infected by superoxide-producing E. faecalis OG1RF and superoxide-deficient strain WY84. Inflammatory cytokines, apoptosis, and biomarkers for the endometrial receptivity were analyzed.

Results: Infection of endometrial epithelial cells with superoxide-producing E. faecalis OG1RF induced expression of inflammatory cytokines, promoted apoptosis, and down-regulated expression of receptivity biomarkers compared to uninfected control. In contrast, superoxide-deficient E. faecalis WY84 had little effect on inflammatory cytokine production, apoptosis, and endometrial receptivity biomarkers.

Conclusions: Extracellular superoxide produced by E. faecalis is an important virulence factor for E. faecalis-induced endometritis leading to reduced receptivity of endometrial epithelial cells.
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http://dx.doi.org/10.1111/aji.13453DOI Listing
May 2021

It takes a village: microbiota, parainflammation, paligenosis and bystander effects in colorectal cancer initiation.

Dis Model Mech 2021 May 10;14(5). Epub 2021 May 10.

Department of Radiation Oncology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

Sporadic colorectal cancer (CRC) is a leading cause of worldwide cancer mortality. It arises from a complex milieu of host and environmental factors, including genetic and epigenetic changes in colon epithelial cells that undergo mutation, selection, clonal expansion, and transformation. The gut microbiota has recently gained increasing recognition as an additional important factor contributing to CRC. Several gut bacteria are known to initiate CRC in animal models and have been associated with human CRC. In this Review, we discuss the factors that contribute to CRC and the role of the gut microbiota, focusing on a recently described mechanism for cancer initiation, the so-called microbiota-induced bystander effect (MIBE). In this cancer mechanism, microbiota-driven parainflammation is believed to act as a source of endogenous mutation, epigenetic change and induced pluripotency, leading to the cancerous transformation of colon epithelial cells. This theory links the gut microbiota to key risk factors and common histologic features of sporadic CRC. MIBE is analogous to the well-characterized radiation-induced bystander effect. Both phenomena drive DNA damage, chromosomal instability, stress response signaling, altered gene expression, epigenetic modification and cellular proliferation in bystander cells. Myeloid-derived cells are important effectors in both phenomena. A better understanding of the interactions between the gut microbiota and mucosal immune effector cells that generate bystander effects can potentially identify triggers for parainflammation, and gain new insights into CRC prevention.
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http://dx.doi.org/10.1242/dmm.048793DOI Listing
May 2021

Association of apolipoproteins A1 and B with type 2 diabetes and fasting blood glucose: a cross-sectional study.

BMC Endocr Disord 2021 Apr 1;21(1):59. Epub 2021 Apr 1.

Scientific Education Section, Affiliated Maternity & Child Health Care Hospital of Nantong University, Nantong, 226018, Jiangsu Province, China.

Background: Apolipoprotein (Apo) may be associated with type 2 diabetes (T2D), however, little is known whether or not serum apolipoproteins are correlated with fasting blood glucose (FBG) and the prevalence of T2D in Chinese populations. In this study, we examined the association of serum ApoA1, ApoB, and the ratio of ApoB/ApoA1 (ApoB/A1 ratio) with T2D and FBG level, and compared apolipoprotein indicators in predicting T2D in Chinese adults.

Methods: A total of 1027 subjects were enrolled in this cross-sectional study. The association of ApoA1, ApoB, and ApoB/A1 ratio with T2D prevalence was determined using logistic regression models. Multivariate-analysis of covariance (ANCOVA) was performed for comparisons of the mean difference in FBG level.

Results: We found that ApoB and ApoB/A1 ratio were positively associated with T2D prevalence and FBG, while inverse association was noted between ApoA1 and T2D prevalence as well as FBG. Stratified analyses for sex, age, body mass index (BMI), smoking, and alcohol consumption showed no significant difference for the association of ApoA1, ApoB, and ApoB/A1 ratio with the prevalence of T2D among subgroups (all p-interactions> 0.05). Nonetheless, ApoA1 poorly performed in predicting T2D as it provided an AUC value of 0.310 that was significantly lower than those observed for ApoB (AUC value: 0.631) and ApoB/A1 ratio (AUC value: 0.685). Finally, path analyses indicated that the association between ApoB and T2D was mediated by BMI.

Conclusions: This study reveals the association of serum ApoA1, ApoB, and ApoB/A1 ratio with T2D and FBG in Chinese adults, suggesting that ApoB and ApoB/A1 ratio may be early indicators for predicting T2D. Prospective investigation in large cohort is needed.
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http://dx.doi.org/10.1186/s12902-021-00726-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017773PMC
April 2021

Synthesis of Biochar Nanoparticles and Their Effects on Growth and Survival of (Gennadius).

Front Microbiol 2021 18;12:630220. Epub 2021 Feb 18.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Research Centre of Biological Control, College of Plant protection, South China Agricultural University, Guangzhou, China.

Nanotechnology can offer an environmentally sustainable alternative to synthetic chemicals for pest management. Nano-formulations of different microbial pest control agents have been effective against several insect pests. Synthesis of -biochar (BC) nanoparticles and their bio-efficacy against was observed during this study. The characterization of BC nanoparticles through different analytical techniques showed successful synthesis of nanoparticles. UV spectroscopy showed a characteristic band of surface plasmon between 350 and 400 nm; SEM images confirmed the synthesis of spherical shaped nanoparticles; X-ray diffractogram showed strong peaks between 2θ values of 20°-25°; and atomic force microscopy (AFM) analysis revealed particle size of 49.151 nm. The bioassay studies demonstrated that different concentrations of BC nanoparticles caused significant reduction in hatchability of eggs as well as survival of immatures emerging from treated eggs when compared with controls. The results also revealed that BC nanoparticles were highly pathogenic against 2nd and 3rd instar nymphs and pupae of having LC values of 6.80, 7.45, and 8.64 ppm, respectively. The LT values for 20 ppm concentration of BC nanoparticles against 2nd and 3rd instar nymphs, and pupae of were 3.25 ± 0.29, 3.69 ± 0.52, and 4.07 ± 0.51 days, respectively. These findings suggest that BC nanoparticles can potentially be used in biorational management programs.
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http://dx.doi.org/10.3389/fmicb.2021.630220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935537PMC
February 2021

New insights into the phylogeny and evolution of lady beetles (Coleoptera: Coccinellidae) by extensive sampling of genes and species.

Mol Phylogenet Evol 2021 03 5;156:107045. Epub 2021 Jan 5.

State Key Laboratory of Biocontrol, College of Ecology and Evolution, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China. Electronic address:

Ladybirds (family Coccinellidae) are one of the most diverse groups of beetles and globally comprise over 6000 species. Despite their scientific and economic significance, the taxonomy of Coccinellidae remains unstable, and we still know little about their evolutionary history. By using a small number of genes, previous phylogenetic analyses have not reliably resolved the relationships among major ladybird lineages. In this study, we sequenced 94 nuclear protein-coding genes for 214 species of Coccinellidae and 14 outgroups, covering 90 genera and 35 tribes. We found that nucleotide compositional heterogeneity is present among ladybird tribes so that phylogenetic inference at the amino acid level is more reliable than at the DNA level. Based on the maximum likelihood analyses of the amino acid dataset, we recognize three subfamilies in Coccinellidae: Microweiseinae, Monocoryninae stat. nov., and Coccinellinae. The subfamily relationships are strongly supported as (Microweiseinae, (Monocoryninae stat. nov., Coccinellinae)). The tribes of ladybirds are mostly monophyletic, except Ortaliini, Sticholotidini, Scymnini, and Coccidulini. The phylogenetic relationships among tribes of Coccinellinae are still not well resolved, with many nodes weakly supported. Our divergence time analysis suggests that the crown group of extant lady beetles arose in the Early Cretaceous ~ 143 million years ago (Mya) and experienced a rapid diversification during the Late Cretaceous (120-70 Mya). We hypothesize that the boom of angiosperms in the Late Cretaceous promoted the diversification of herbivorous Sternorrhyncha insects, especially aphids, which in turn drove the rapid radiation of predatory lady beetles. In summary, our work provides a comprehensive time-calibrated phylogeny of Coccinellidae that provides a sound framework for revising their classification and understanding the origin of their biodiversity.
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http://dx.doi.org/10.1016/j.ympev.2020.107045DOI Listing
March 2021

A "turn-on" and proximity ligation assay dependent DNA tweezer for one-step amplified fluorescent detection of DNA.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 11;249:119292. Epub 2020 Dec 11.

School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China. Electronic address:

A "turn-on" and proximity ligation assay dependent DNA tweezer was proposed for one-step amplified fluorescent detection of DNA. Target DNA can anneal with capture probe to form an entire long sequence. The formed long sequence can circularly open the hairpin, resulting the "turn-on" of DNA tweezers. A good linear relationship was shown from 40 pM to 20 nM with limit of detection of 10 pM. In addition, it has been successfully utilized to analysis DNA in human serum, representing a great and practical application future.
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http://dx.doi.org/10.1016/j.saa.2020.119292DOI Listing
March 2021

Structural Studies of Giant Empty and Endohedral Fullerenes.

Front Chem 2020 3;8:607712. Epub 2020 Dec 3.

Department of Chemistry, Moscow State University, Moscow, Russia.

Structure elucidations of giant fullerenes composed of 100 or more carbon atoms are severely hampered by their extremely low yield, poor solubility and huge numbers of possible cage isomers. High-temperature exohedral chlorination followed by X-ray single crystal diffraction studies of the chloro derivatives offers a practical solution for structure elucidations of giant fullerenes. Various isomers of giant fullerenes have been determined by this method, specially, non-classical giant fullerenes containing heptagons generated by the skeletal transformations of carbon cages. Alternatively, giant fullerenes can be also stabilized by encapsulating metal atoms or clusters through intramolecular electron transfer from the encapsulated species to the outer fullerene cage. In this review, we present a comprehensive overview on synthesis, separation and structural elucidation of giant fullerenes. The isomer structures, chlorination patterns of a series of giant fullerenes C (2 = 100-108) and heptagon-containing non-classical fullerenes derived from giant fullerenes are summarized. On the other hand, giant endohedral fullerenes bearing different endohedral species are also discussed. At the end, we propose an outlook on the future development of giant fullerenes.
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http://dx.doi.org/10.3389/fchem.2020.607712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744686PMC
December 2020

A contribution to the genus Sphaeroplotina Miyatake (Coleoptera, Coccinellidae, Plotinini).

Zootaxa 2020 Oct 7;4859(3):zootaxa.4859.3.7. Epub 2020 Oct 7.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Department of Entomology, South China Agricultural University; Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture; Guangzhou, 510640, China..

The genus Sphaeroplotina Miyatake, 1969 is reviewed. Sphaeroplotina hainanensis Miyatake, 1969 is redescribed and Sphaeroplotina varimarginata Tong Wang sp. nov. is described as new to science. Both species are illustrated. A diagnosis of the genus is provided. A key and a distribution map of the known species are also presented.
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http://dx.doi.org/10.11646/zootaxa.4859.3.7DOI Listing
October 2020

The genus Cryptogonus Mulsant (Coleoptera: Coccinellidae) from Pakistan, with description of a new species and a new record.

Zootaxa 2020 Oct 15;4861(1):zootaxa.4861.1.11. Epub 2020 Oct 15.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Department of Entomology, South China Agricultural University, Guangzhou 510640, China..

The new species of a ladybird beetle, Cryptogonus bhalwalnensis Azad Wang sp. nov. is described from Pakistan. Cryptogonus nepalensis bhutanensis Bielawski is recorded for the first time from Pakistan. Both species of Cryptogonus from Pakistan are diagnosed and illustrated; a distribution map is also presented.
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http://dx.doi.org/10.11646/zootaxa.4861.1.11DOI Listing
October 2020

Optimizing the widely used nuclear protein-coding gene primers in beetle phylogenies and their application in the genus Vandenberg (Coleoptera: Coccinellidae).

Ecol Evol 2020 Jul 28;10(14):7731-7738. Epub 2020 Jun 28.

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm Department of Forest Protection College of Forestry and Landscape Architecture South China Agricultural University Guangzhou China.

Advances in genomic biology and the increasing availability of genomic resources allow developing hundreds of nuclear protein-coding (NPC) markers, which can be used in phylogenetic research. However, for low taxonomic levels, it may be more practical to select a handful of suitable molecular loci for phylogenetic inference. Unfortunately, the presence of degenerate primers of NPC markers can be a major impediment, as the amplification success rate is low and they tend to amplify nontargeted regions. In this study, we optimized five NPC fragments widely used in beetle phylogenetics (i.e., two parts of carbamoyl-phosphate synthetase: CADXM and CADMC, Topoisomerase, Wingless and Pepck) by reducing the degenerate site of primers and the length of target genes slightly. These five NPC fragments and 6 other molecular loci were amplified to test the monophyly of the coccinellid genus Vandenberg. The analysis of our molecular data set clearly supported the genus may be monophyletic but confirmation with an extended sampling is required. A fossil-calibrated chronogram was generated by BEAST, indicating an origin of the genus at the end of the Cretaceous (77.87 Myr). Furthermore, a phylogenetic informativeness profile was generated to compare the phylogenetic properties of each gene more explicitly. The results showed that COI provides the strongest phylogenetic signal among all the genes, but Pepck, Topoisomerase, CADXM and CADMC are also relatively informative. Our results provide insight into the evolution of the genus , and also enrich the molecular data resources for further study.
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http://dx.doi.org/10.1002/ece3.6497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391345PMC
July 2020

New records and checklist of Chilocorini (Coleoptera: Coccinellidae) from China.

Biodivers Data J 2020 24;8:e51092. Epub 2020 Jun 24.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Engineering Research Center of Biological Control, Ministry of Education & Guangdong Province, South China Agricultural University, Guangzhou, China Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Engineering Research Center of Biological Control, Ministry of Education & Guangdong Province, South China Agricultural University Guangzhou China.

Background: China is one of the countries with the greatest species diversity of Chilocorini (Coleoptera: Coccinellidae), including nearly forty-five percent of the known genera and fourteen percent of all described species in this tribe. Recently, we discovered three species previously not recorded in China.

New Information: In this study, three species (Mulsant, 1853), Canepari, 1997 and (Gebler, 1830) are documented for the first time in China. is the first member of the genus Mulsant, 1850 recorded in China. Detailed descriptions, illustrations and distributions of these three species are provided. A checklist of Chinese Chilocorini is also given.
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http://dx.doi.org/10.3897/BDJ.8.e51092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329919PMC
June 2020

An integrative DNA barcoding framework of ladybird beetles (Coleoptera: Coccinellidae).

Sci Rep 2020 06 22;10(1):10063. Epub 2020 Jun 22.

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm; Department of Forest Protection, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510640, China.

Even though ladybirds are well known as economically important biological control agents, an integrative framework of DNA barcoding research was not available for the family so far. We designed and present a set of efficient mini-barcoding primers to recover full DNA barcoding sequences for Coccinellidae, even for specimens collected 40 years ago. Based on these mini-barcoding primers, we obtained 104 full DNA barcode sequences for 104 species of Coccinellidae, in which 101 barcodes were newly reported for the first time. We also downloaded 870 COI barcode sequences (658 bp) from GenBank and BOLD database, belonging to 108 species within 46 genera, to assess the optimum genetic distance threshold and compare four methods of species delimitation (GMYC, bPTP, BIN and ABGD) to determine the most accurate approach for the family. The results suggested the existence of a 'barcode gap' and that 3% is likely an appropriate genetic distance threshold to delimit species of Coccinellidae using DNA barcodes. Species delimitation analyses confirm ABGD as an accurate and efficient approach, more suitable than the other three methods. Our research provides an integrative framework for DNA barcoding and descriptions of new taxa in Coccinellidae. Our results enrich DNA barcoding public reference libraries, including data for Chinese coccinellids. This will facilitate taxonomic identification and biodiversity monitoring of ladybirds using metabarcoding.
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http://dx.doi.org/10.1038/s41598-020-66874-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308296PMC
June 2020

Three new species of the genus Miyatake (Coleoptera, Coccinellidae, Sticholotidini) from the Philippines.

Zookeys 2020 1;937:115-127. Epub 2020 Jun 1.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Ministry of Education & Guangdong Province; Department of Entomology, South China Agricultural University, Guangzhou 510640, China South China Agricultural University Guangzhou China.

The genus Miyatake, 1994 has been revised. Three new species ( Zhang & Wang, , Zhang & Wang, , and Zhang & Wang, ) from the Philippines are described and illustrated in the present paper. An updated key to the species of the genus is provided. In addition, a list of all known species and their distributions is also provided.
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http://dx.doi.org/10.3897/zookeys.937.50139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285854PMC
June 2020

A Case of Congenital Nephrogenic Diabetes Insipidus Caused by Thr108Met Variant of Aquaporin 2.

Front Pediatr 2020 30;8:15. Epub 2020 Jan 30.

Department of Pediatric Nephrology, Lanzhou University Second Hospital, Lanzhou, China.

Congenital nephrogenic diabetes insipidus (CNDI) is a rare renal disorder caused by mutations in arginine vasopressin receptor 2 (AVPR2) or aquaporin 2 (AQP2). The clinical signs of CNDI include polyuria, compensatory polydipsia, dehydration, electrolyte disorder, and developmental retardation without prompt treatment. In this study we report a rare case of CNDI caused by a single base transition in gene. A 4.5 years old male patient suffered from oral dryness, polydipsia, and polyuria for more than 3 years. Laboratory examinations showed hypernatremia, hyperchloremia, and decreased urine osmolality and specific gravity. Ultrasound and MRI found bilateral upper ureteral dilatation and hydronephrosis. Furthermore, sequencing analysis found a C>T transition leading to a T108M missense mutation of AQP2. The patient was given low sodium diet and treated with hydrochlorothiazide followed by amiloride with indomethacin. The patient's clinical course improved remarkably after 1 year of treatment. This study reports the first case of CNDI featuring T108M missense mutation alone. These findings demonstrate a causative role of T108M mutation for CNDI and contribute to the mechanistic understanding of CNDI disease process.
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http://dx.doi.org/10.3389/fped.2020.00015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002472PMC
January 2020

Risks associated with enterococci as probiotics.

Food Res Int 2020 03 21;129:108788. Epub 2019 Nov 21.

Department of Radiation Oncology and Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

Probiotics are naturally occurring microorganisms that confer health benefits by altering host commensal microbiota, modulating immunity, enhancing intestinal barrier function, or altering pain perception. Enterococci are human and animal intestinal commensals that are used as probiotics and in food production. These microorganisms, however, express many virulence traits including cytolysin, proteases, aggregation substance, capsular polysaccharide, enterococcal surface protein, biofilm formation, extracellular superoxide, intestinal translocation, and resistance to innate immunity that can lead to serious hospital-acquired infections. In addition, enterococci are facile in acquiring antibiotic resistance genes to many clinically important antibiotics encoded on a wide variety of conjugative plasmids, transposons, and bacteriophages. The pathogenicity and disease burden caused by enterococci render them poor choices as probiotics. No large, randomized, placebo-controlled clinical trials have demonstrated the safety and efficacy of any enterococcal probiotic. As a result, no enterococcal probiotic has been approved by the United States Food and Drug Administration for the treatment, cure, or amelioration of human disease. In 2007, the European Food Safety Authority concluded that enterococci do not meet the standard for "Qualified Presumption of Safety". Enterococcal strains used or proposed for use as probiotics should be carefully screened for efficacy and safety.
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http://dx.doi.org/10.1016/j.foodres.2019.108788DOI Listing
March 2020

A simple and programmed DNA tweezer probes for one-step and amplified detection of UO.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Mar 30;229:118017. Epub 2019 Dec 30.

School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China. Electronic address:

A simple DNA tweezer was proposed for one-step and amplified detection of UO based on DNAzyme catalytic cleavage. The two arms of DNA tweezers are close in the original form. Thus, the fluorescent signal of fluorophore at the end of arm is dramatically quenched. However, the structure of DNA tweezers can be changed from "close" to "open" in the presence of UO, resulting the strong fluorescent signal. The linear range was obtained in the range of 0.1 nM to 60 nM and the limit of detection was 25 pM with the amplification of DNAzyme catalytic cleavage reaction. Importantly, the whole detection process is very simple and only one operation step is required. In addition, it shows great potential and promising prospects for uranyl detection in practical application.
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http://dx.doi.org/10.1016/j.saa.2019.118017DOI Listing
March 2020

Three new species of Shirozuella Sasaji (Coleoptera, Coccinellidae) from China.

Zootaxa 2019 Jun 12;4615(1):zootaxa.4615.1.11. Epub 2019 Jun 12.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Department of Entomology, South China Agricultural University, Guangzhou, 510640, China..

Three new species of Shirozuella Sasaji, 1967 are described from Yunnan, China, Shirozuella flavosemiovata sp. n., Shirozuella limbata sp. n. and Shirozuella poststigmaea sp. n. A distribution map, diagnoses and detailed descriptions for these new species are provided. A key to the known species from China is updated.
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http://dx.doi.org/10.11646/zootaxa.4615.1.11DOI Listing
June 2019

An iron-carbon-activated carbon and zeolite composite filter, anaerobic-aerobic integrated denitrification device for nitrogen removal in low C/N ratio sewage.

Water Sci Technol 2019 Jul;80(2):223-231

Modern Agricultural Science and Engineering Institute, Tongji University, Shanghai 200092, China and Chongqing Key Laboratory of Catalysis & Functional Organic Molecules, Chongqing 400067, China E-mail:

In this study, we use an anaerobic-aerobic integrated denitrification (Fe/C-ZACID) device with an iron-carbon-activated carbon and zeolite composite filter to remove nitrogen from simulated low carbon-nitrogen ratio (C/N) sewage. The impacts of dissolved oxygen (DO) level, hydraulic retention time (HRT), C/N and nitrate recirculation ratio on denitrification performance were studied. The results show that when HRT was 6 h, DO was 3 ± 0.1 mg/L, influent C/N was 3, and nitrate recirculation ratio was 100%, and removal rates of 95% for ammonia and 85% for total nitrogen (TN) were achieved. A beaker comparison test demonstrated that this synergistic denitrification system included heterotrophic denitrification, physicochemical denitrification, iron autotrophic denitrification and hydrogen autotrophic denitrification, etc. The Fe/C-ZACID device has a high-efficiency nitrogen removal effect for low C/N ratio sewage and strong shock resistance, which provides technical support and a theoretical basis for advanced denitrification of rural domestic sewage.
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http://dx.doi.org/10.2166/wst.2019.261DOI Listing
July 2019

Dual-entropy-driven catalytic amplification reaction for ultra-sensitive and visible detection of Hg in water based on thymine-Hg-thymine coordination chemistry.

Analyst 2019 Aug;144(17):5143-5149

School of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, 400067, China.

An ultra-sensitive and visible Hg2+ detection strategy was established. It was based on T-Hg2+-T coordination chemistry and a dual-entropy-driven catalytic reaction (EDCR). The dual EDCR was initiated by T-Hg2+-T coordination chemistry, resulting in the release of a G-rich sequence to form a hemin/G-quadruplex-HRP-mimicking DNAzyme, and then catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) into TMB+, with a color change from colorless to blue. This method showed great sensitivity and excellent selectivity, and the limit of detection reached 0.6 pM. The feasibility of this method was demonstrated by real water samples. Moreover, the visible color change provided the possibility of ultra-sensitive detection of Hg2+ by the naked eye.
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http://dx.doi.org/10.1039/c9an00882aDOI Listing
August 2019

Thymine-Hg-thymine coordination chemistry induced entropy driven catalytic reaction to form Hemin/G-quadruplex-HRP-mimicking DNAzyme for colorimetric and visual determination of Hg.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Nov 31;222:117228. Epub 2019 May 31.

School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China. Electronic address:

A sensitive and visible colorimetric strategy was proposed for Hg detection by thymine-Hg-thymine (T-Hg-T) coordination chemistry and entropy driven catalytic reaction. The entropy driven catalytic reaction is induced by T-Hg-T coordination chemistry, resulting the releasing of G-riched sequence. Hemin/G-quadruplex-HRP-mimicking DNAzyme can be formed with the help of hemin, catalyzing TMB to TMB with a color change from colorless to blue. The sensitivity of this strategy can be reached to 2 pM, which is significantly improved by entropy driven catalytic reaction. In addition, entropy driven catalytic reaction provides a more reliable and accurate results. This method shows great promise for on-site analysis and in-house diagnosis of Hg in water.
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http://dx.doi.org/10.1016/j.saa.2019.117228DOI Listing
November 2019

Effects of Different Pretreatments of DNA Extraction from Dried Specimens of Ladybird Beetles (Coleoptera: Coccinellidae).

Insects 2019 Mar 29;10(4). Epub 2019 Mar 29.

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Department of Forest Protection, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510640, China.

Obtaining genetic information from museum specimens is a fundamental component of many fields of research, including DNA barcoding, population genetics, conservation genetics, and phylogenetic analysis. However, acquiring genetic information from museum specimens is challenging because of the difficulty in amplifying the target sequences due to DNA damage and degradation. Different pretreatments can significantly impact the purity and concentration of genomic DNA from museum specimens. Here, we assessed four pretreatment methods-use of 0.9% NaCl buffer, phosphate-buffered saline (PBS), Saline Tris-EDTA (STE) buffer, and sterile water-to determine which pretreatment is most suitable for DNA extraction from dried specimens of ladybird beetles. We completed a comprehensive phylogenetic analysis to test whether the sequences obtained from dried specimens enable proper phylogenetic inference. Our results showed that pretreatment can improve the quality of DNA from dried specimens. The pretreatment effects of 0.9% NaCl buffer and STE buffer were better than those of PBS buffer and sterile water. The phylogenetic analyses results showed that museum specimens can be used to generate cogent phylogenetic inferences. We report the optimum pretreatment methods for DNA extraction from dried ladybird beetles specimens as well as provide evidence for accurately determining phylogenetic relationships for museum specimens.
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http://dx.doi.org/10.3390/insects10040091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523959PMC
March 2019

Isaria fumosorosea-based zero-valent iron nanoparticles affect the growth and survival of sweet potato whitefly, Bemisia tabaci (Gennadius).

Pest Manag Sci 2019 Aug 10;75(8):2174-2181. Epub 2019 Mar 10.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Research Centre of Biological Control, South China Agricultural University, Guangzhou, P.R. China.

Background: Nanoparticles can be used for effective pest management as a combined formulation of metal and some other material that has proven efficacy against a given pest. This study reports the synthesis, characterization and efficacy of Isaria fumosorosea-based zero-valent iron (ZVI) nanoparticles against sweet potato whitefly Bemisia tabaci (Gennadius).

Results: The I. fumosorosea-ZVI nanoparticles showed a characteristic surface plasmon absorption band at 470 nm during UV-visible spectroscopy. The scanning electron micrographs of nanoparticles showed spherical shaped nanoparticles with sizes ranging between 1.71 and 3.0 µm. The EDX analysis showed the characteristic peak of iron at 0.6 and 6.8 KeV. The XRD analysis showed characteristic peaks at 44.72°, 65.070°, 82.339° and 82.65°. The bioassay results indicated that the percentage of larval mortality of B. tabaci challenged with I. fumosorosea ZVI nanoparticles was both concentration and age dependent. Isaria fumosorosea ZVI nanoparticles showed high pathogenicity against second and third instar nymphs, and pupae with LC values of 19.17, 26.10 and 37.71 ppm, respectively. The LT was lowest for second instar nymphs (3.15 days) and highest for pupae (4.22 days) when inoculated with a concentration of 50 ppm.

Conclusion: Isaria fumosorosea ZVI nanoparticles can be an eco-friendly tool for effective B. tabaci management. © 2019 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.5340DOI Listing
August 2019

Contribution to the genus Miyatake, 1994 (Coleoptera, Coccinellidae, Sticholotidini).

Zookeys 2018 29(793):135-142. Epub 2018 Oct 29.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Department of Entomology, South China Agricultural University, Guangzhou 510640, China South China Agricultural University Guangzhou China.

The genus Miyatake has been reviewed in this study. Descriptions and illustrations of two species ( Miyatake and Wang, Zhang & Ślipiński, ) in the Luzon island of the Philippines, are given. The male genitalia of are described for the first time. A key to known species is also provided.
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http://dx.doi.org/10.3897/zookeys.793.24790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218531PMC
October 2018

Commensal-infected macrophages induce dedifferentiation and reprogramming of epithelial cells during colorectal carcinogenesis.

Oncotarget 2017 Nov 1;8(60):102176-102190. Epub 2017 Nov 1.

The Muchmore Laboratories for Infectious Diseases Research, Oklahoma City VA Health Care System, Oklahoma City, OK 73104, USA.

The colonic microbiome contributes to the initiation of colorectal cancer through poorly characterized mechanisms. We have shown that commensal-polarized macrophages induce gene mutation, chromosomal instability, and endogenous transformation through microbiome-induced bystander effects (MIBE). In this study we show that MIBE activates Wnt/β-catenin signaling and pluripotent transcription factors associated with dedifferentiation, reprogramming, and the development of colorectal cancer stem cells (CSCs). Exposure of murine primary colon epithelial cells (YAMC) to -infected macrophages increased Wnt3α expression while suppressing Wnt inhibitor factor 1 (Wif1). Wnt/β-catenin activation was confirmed by increased active β-catenin and Tcf4. , active β-catenin was evident in colon biopsies from -colonized knockout mice compared to sham-colonized mice. This effect was mediated, in part, by 4-hydroxy-2-nonenal and tumor necrosis factor α. MIBE also activated pluripotent transcription factors c-Myc, Klf4, Oct4, and Sox2 in YAMC cells and colons from -colonized knockout mice. These transcription factors are associated with cellular reprogramming, dedifferentiation, and induction of colorectal CSC progenitors. In support of this was an increase in the expression of Dclk1 and CD44, two colorectal CSC markers, in YAMC cells that were exposed to MIBE. Finally, compared to normal colon biopsies and hyperplastic polyps, DCLK1 expression increased in human tubular adenomas and invasive colorectal cancers. Blocking β-catenin/TCF4 signaling using FH535 and -specific small interfering RNA decreased DCLK1 expression in HCT116 human colon cancer cells. These findings provide mechanism for microbiome-induced colorectal cancer and identify new potential targets for colorectal cancer prevention.
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http://dx.doi.org/10.18632/oncotarget.22250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731944PMC
November 2017

A new species of the subgenus Scymnus from Pakistan (Coleoptera, Coccinellidae).

Zookeys 2017 29(694):31-39. Epub 2017 Aug 29.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Department of Entomology, South China Agricultural University, Guangzhou 510640, China.

A new species, Scymnus (Scymnus) contortubus Rashid, Chen & Wang, , is described and illustrated from Pakistan. A diagnosis, remarks, illustrations, and a distribution map are provided of the new species and its most similar congener, S. (S..) nubilus Mulsant.
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http://dx.doi.org/10.3897/zookeys.694.12863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5672776PMC
August 2017

, a new species of the genus Kapur, 1948 from the Malay Peninsula (Coleoptera, Coccinellidae).

Zookeys 2017 4(706):109-115. Epub 2017 Oct 4.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; College of Agriculture, South China Agricultural University, Guangzhou 510640, China.

A new species of the genus Kapur, 1948 (Coleoptera: Coccinellidae), Huo & Wang, from the Malay Peninsula is described with illustrations and a distribution map. The genus is recorded for the first time from Malaysia and Singapore.
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http://dx.doi.org/10.3897/zookeys.706.18081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674088PMC
October 2017

Epiverta Dieke (Coleoptera: Coccinellidae: Epilachnini): A Complex of Species, Not a Monotypic Genus.

J Insect Sci 2017 Jan;17(2)

Engineering Research Centre of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.

Rich sampling and modern research techniques, including SEM, revealed that rarely collected epilachnine species Epiverta chelonia is a complex of four closely related species: E. chelonia (Mader, 1933), E. albopilosa, E. angusta, and E. supinata spp. nov. All Epiverta species are described and illustrated, a key to the species and a distribution map are provided. Lectotype of Solanophila cheloniaMader, 1933 is designated and its type locality delimited to Yunnan Province, Deqin County (China).
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http://dx.doi.org/10.1093/jisesa/iex027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5416896PMC
January 2017

, a new genus and species of Chilocorini from Tibet, China (Coleoptera, Coccinellidae).

Zookeys 2017 7(678):121-128. Epub 2017 Jun 7.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Department of Entomology, South China Agricultural University, Guangzhou 510640, China.

A new monotypic genus of Chilocorini, Li & Wang, , with a new species Li et Wang, . is described from Tibet, China. All diagnostic features are illustrated. The relationships with other genera of Chilocorini are discussed and a key to Chinese genera of Chilocorini is provided.
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http://dx.doi.org/10.3897/zookeys.678.11862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5523365PMC
June 2017

Molecular phylogeny reveals food plasticity in the evolution of true ladybird beetles (Coleoptera: Coccinellidae: Coccinellini).

BMC Evol Biol 2017 06 26;17(1):151. Epub 2017 Jun 26.

Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warszawa, Poland.

Background: The tribe Coccinellini is a group of relatively large ladybird beetles that exhibits remarkable morphological and biological diversity. Many species are aphidophagous, feeding as larvae and adults on aphids, but some species also feed on other hemipterous insects (i.e., heteropterans, psyllids, whiteflies), beetle and moth larvae, pollen, fungal spores, and even plant tissue. Several species are biological control agents or widespread invasive species (e.g., Harmonia axyridis (Pallas)). Despite the ecological importance of this tribe, relatively little is known about the phylogenetic relationships within it. The generic concepts within the tribe Coccinellini are unstable and do not reflect a natural classification, being largely based on regional revisions. This impedes the phylogenetic study of important traits of Coccinellidae at a global scale (e.g. the evolution of food preferences and biogeography).

Results: We present the most comprehensive phylogenetic analysis of Coccinellini to date, based on three nuclear and one mitochondrial gene sequences of 38 taxa, which represent all major Coccinellini lineages. The phylogenetic reconstruction supports the monophyly of Coccinellini and its sister group relationship to Chilocorini. Within Coccinellini, three major clades were recovered that do not correspond to any previously recognised divisions, questioning the traditional differentiation between Halyziini, Discotomini, Tytthaspidini, and Singhikaliini. Ancestral state reconstructions of food preferences and morphological characters support the idea of aphidophagy being the ancestral state in Coccinellini. This indicates a transition from putative obligate scale feeders, as seen in the closely related Chilocorini, to more agile general predators.

Conclusions: Our results suggest that the classification of Coccinellini has been misled by convergence in morphological traits. The evolutionary history of Coccinellini has been very dynamic in respect to changes in host preferences, involving multiple independent host switches from different insect orders to fungal spores and plants tissues. General predation on ephemeral aphids might have created an opportunity to easily adapt to mixed or specialised diets (e.g. obligate mycophagy, herbivory, predation on various hemipteroids or larvae of leaf beetles (Chrysomelidae)). The generally long-lived adults of Coccinellini can consume pollen and floral nectars, thereby surviving periods of low prey frequency. This capacity might have played a central role in the diversification history of Coccinellini.
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http://dx.doi.org/10.1186/s12862-017-1002-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485688PMC
June 2017

Transfer of lead (Pb) in the soil-plant-mealybug-ladybird beetle food chain, a comparison between two host plants.

Ecotoxicol Environ Saf 2017 Sep 27;143:289-295. Epub 2017 May 27.

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Research Centre of Biological Control, South China Agricultural University, Guangzhou 510642, P.R. China; Guangdong Engineering Research Centre of Microbial Pesticides, Guangdong New Scene Biological Engineering Co. Ltd, Yangjiang 529932, P.R. China. Electronic address:

Contamination of soil with heavy metals has become an issue of concern on global scale. This study investigates the translocation of lead (Pb) along the soil - plant (eggplant and tomato) - mealybug (Dysmicoccus neobrevipes) - ladybird beetle (Cryptolaemus montrouzieri) food chain. Soil amendments used for this study were adjusted to 0, 25, 50 and 100mg/kg of Pb (w/w). The results revealed significantly higher transfer of Pb in tomato when compared to eggplant. Bio-magnification of Pb (2-4 times) was observed for soil - root transfer whereas Pb was bio-minimized in later part of food chain (shoot - mealybug - ladybird transfer). A dose dependent increase in transfer of Pb across the multi-trophic food chain was observed for both host plants. A decrease in coefficients of Pb transfer (from root - shoot and shoot - mealybug) was observed with increase in Pb concentrations. Our results also showed removal of Pb from the bodies of ladybird beetle during metamorphosis. Further studies are required to explain the mechanisms or physiological pathways involved in the bio-minimization of Pb across the food chain.
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http://dx.doi.org/10.1016/j.ecoenv.2017.05.032DOI Listing
September 2017