Publications by authors named "Xing Wu"

473 Publications

Boosting with heterologous vaccines effectively improves protective immune responses of the inactivated SARS-CoV-2 vaccine.

Emerg Microbes Infect 2021 Jul 18:1-28. Epub 2021 Jul 18.

National Institutes for Food and Drug Control, No.31, Huatuo Road, Daxing District, Beijing 102629, People's Republic of China.

Since the outbreak of COVID-19, a variety of vaccine platforms have been developed. Amongst these, inactivated vaccines have been authorized for emergency use or conditional marketing in many countries. To further enhance the protective immune responses in populations that have completed vaccination regimen, we investigated the immunogenic characteristics of different vaccine platforms and tried homologous or heterologous boost strategy post two doses of inactivated vaccines in a mouse model. Our results showed that the humoral and cellular immune responses induced by different vaccines when administered individually differ significantly. In particular, inactivated vaccines showed relatively lower level of neutralizing antibody and T cell responses, but a higher IgG2a/IgG1 ratio compared with other vaccines. Boosting with either recombinant subunit, adenovirus vectored or mRNA vaccine after two-doses of inactivated vaccine further improved both neutralizing antibody and Spike-specific Th1-type T cell responses compared to boosting with a third dose of inactivated vaccine. Our results provide new ideas for prophylactic inoculation strategy of SARS-CoV-2 vaccines.
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http://dx.doi.org/10.1080/22221751.2021.1957401DOI Listing
July 2021

LncRNA NEAT1 mediates intestinal inflammation by regulating TNFRSF1B.

Ann Transl Med 2021 May;9(9):773

Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha, China.

Background: Inflammatory bowel disease (IBD) is a chronic nonspecific intestinal disease. Our previous work showed that long non-coding RNA (LncRNA) nuclear enriched abundant transcript 1 (NEAT1) plays an important role in IBD. In the current study, we aimed to explore the underlying mechanism by which NEAT1 participates in the development of the disease.

Methods: Real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting were used to detect the expression of NEAT1 and tumor necrosis factor superfamily member 1B (TNFRSF1B) in clinical specimens and dextran sulfate sodium (DSS) colitis mice. Inflammatory cell models were established by stimulating human normal intestinal epithelial cell line NCM460 and human colon cancer cell line HT-29 with tumor necrosis factor alpha (TNF-α). Expressions of inflammatory cytokines such as interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) were detected by enzyme-linked immunosorbent assay (ELISA) or RT-qPCR, TNFRSF1B, NF-κB p65 and p-NF-κB p65 followed by the knockdown or overexpression of NEAT1 and TNFRSF1B were analyzed by western blotting, and the regulatory effects of NEAT1 on TNFRSF1B were detected by RNA pull-down experiments and RNA-decay assay. The translocation of NF-κB p65 to the nucleus was detected by immunofluorescence.

Results: In patients' specimens and DSS colitis mouse models, NEAT1 and TNFRSF1B expression were up-regulated compared with the control group. TNF-α stimulation increased NEAT1 and TNFRSF1B expression and activated NF-κB signaling pathway by increasing the translocation of NF-κB p65 to the nucleus. In the presence of TNF-α stimulation, NEAT1 knockdown reduces the expression of TNFRSF1B and the translocation of NF-κB p65, thereby relieves cell inflammation. These effects can be reversed by the overexpression of TNFRSF1B.In addition, NEAT1 is involved in inflammatory response by up-regulating the mRNA levels of TNFRSF1B, and knocking down NEAT1 can alleviate inflammation by down-regulating TNFRSF1B. Moreover, NEAT1 co-precipitates TNFRSF1B mRNA in RNA-pulldown assay, and the presence of NEAT1 stabilizes the mRNA of TNFRSF1B.

Conclusions: Our results showed that LncRNA NEAT1 promotes NF-κB p65 translocation and mediates intestinal inflammation by regulating TNFRSF1B.
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http://dx.doi.org/10.21037/atm-21-34DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246228PMC
May 2021

Prediction of Crack Resistance of LFSMA-13 with and without Anti-Rut Agent Using Parameters of FTIR Spectrum under Different Aging Degrees.

Materials (Basel) 2021 Jun 10;14(12). Epub 2021 Jun 10.

Jiangsu Expressway Company Limited, Nanjing 210000, China.

This paper aims to better analyze the crack resistance of lignin fiber reinforced SMA-13 (LFSMA-13) asphalt mixtures, with and without polymer anti-rut agent (ARA), under different aging degrees. IDEAL-CT test and Fourier transform infrared (FTIR) spectroscopy were utilized to analyze the relationships between the crack resistance of LFSMA-13, with and without ARA, and the parameters of the FTIR spectrum of the asphalt extracted from the test samples. A convenient testing method to predict the anti-crack ability of the mixtures in a road was also derived in this study. The test samples were prepared using the specifications listed by AASHTO. The fracture formation work (W) and cracking index (CT) in the IDEAL-CT test were adopted to reflect the cracking ability of the asphalt mixtures in both the crack formation stage and the crack propagation stage. The peak areas of the FTIR spectrum were utilized to reveal the chemical properties of the asphalt material inside the SMA-13 asphalt mixtures, with and without ARA under different aging degrees. Grey correlation analysis was adopted to choose the most suitable FTIR spectrum parameters to derive the prediction models of W and CT under different aging degrees. After conducting a series of tests, the results showed that the aging process could well affect the crack resistance of the test samples and the peak areas of the asphalt extracted from the mixtures. The FTIR parameters selected from the grey correlation analysis could be used to well predict the anti-crack ability of the asphalt mixtures.
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http://dx.doi.org/10.3390/ma14123209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230506PMC
June 2021

LncAY controls BMI1 expression and activates BMI1/Wnt/β-catenin signaling axis in hepatocellular carcinoma.

Life Sci 2021 Sep 24;280:119748. Epub 2021 Jun 24.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, NHC Key Laboratory of Glycoconjugates Research (Fudan University), Shanghai, PR China. Electronic address:

Background & Aims: Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver. Long non-coding RNAs as master gene regulators play important roles in tumorigenesis and progression. However, the significance of lncRNAs and their regulatory mechanisms in HCC are largely unknown. Our study was to define the role of lncAY (long noncoding RNA AY927503) in HCC.

Methods: Methylated RNA immunoprecipitation qPCR combined with bioinformatics were used to identify the m6A modification of lncAY. qRT-PCR, western blotting and immunofluorescence were used to identify the expression of the lncAY/YTHDF2/BMI1/Wnt axis in HCC tissues and cell lines. Gain- and loss-of functions of lncAY and BMI1 were implemented to confirm their roles in the behaviors of HCC cells.

Results: Our findings suggested that m6A-modified lncAY expression relied on m6A "reader" protein YTHDF2. LncAY upregulated BMI1 expression in HCC cells and a notably positive relevance is evident between lncAY and BMI1 expression in TCGA HCC datasets. BMI1 was upregulated in HCC tissues and patients with higher BMI1 expression had a poor clinical prognosis. Besides, GSEA analysis showed remarkable enrichment of high BMI1 expression in gene sets associated with Wnt/β-catenin signaling. Rescue results revealed that BMI1 reversed the suppressive effects of lncAY depletion in HCC cells.

Conclusions: Our work suggested that lncAY might elevate BMI1 expression and further activate the Wnt/β-catenin signaling. BMI1 reverses the suppressive effects of lncAY depletion in HCC cells. Collectively, our work uncovers a novel undefined regulatory signaling pathway, namely lncAY/BMI1/Wnt/β-catenin axis, involved in liver cancer progression.
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http://dx.doi.org/10.1016/j.lfs.2021.119748DOI Listing
September 2021

Suppression of the optical crosstalk in a multi-channel silicon photomultiplier array.

Opt Express 2021 May;29(11):16914-16926

We propose and study a method of optical crosstalk suppression for silicon photomultipliers (SiPMs) using optical filters. We demonstrate that attaching absorptive visible bandpass filters to the SiPM can substantially reduce the optical crosstalk. Measurements suggest that the absorption of near infrared light is important to achieve this suppression. The proposed technique can be easily applied to suppress the optical crosstalk in SiPMs in cases where filtering near infrared light is compatible with the application.
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http://dx.doi.org/10.1364/OE.424460DOI Listing
May 2021

Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19.

Synth Syst Biotechnol 2021 Sep 14;6(3):135-143. Epub 2021 Jun 14.

Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510630, China.

SARS-CoV-2, the causative agent for COVID-19, infect human mainly via respiratory tract, which is heavily inhabited by local microbiota. However, the interaction between SARS-CoV-2 and nasopharyngeal microbiota, and the association with metabolome has not been well characterized. Here, metabolomic analysis of blood, urine, and nasopharyngeal swabs from a group of COVID-19 and non-COVID-19 patients, and metagenomic analysis of pharyngeal samples were used to identify the key features of COVID-19. Results showed lactic acid, l-proline, and chlorogenic acid methyl ester (CME) were significantly reduced in the sera of COVID-19 patients compared with non-COVID-19 ones. Nasopharyngeal commensal bacteria including , and were notably depleted in the pharynges of COVID-19 patients, while , , and were relatively increased. The abundance of and were significantly positively associated with serum CME, which might be an anti-SARS-CoV-2 bacterial metabolite. This study provides important information to explore the linkage between nasopharyngeal microbiota and disease susceptibility. The findings were based on a very limited number of patients enrolled in this study; a larger size of cohort will be appreciated for further investigation.
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http://dx.doi.org/10.1016/j.synbio.2021.06.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200311PMC
September 2021

Geniposide ameliorates chronic unpredictable mild stress induced depression-like behavior through inhibition of ceramide-PP2A signaling via the PI3K/Akt/GSK3β axis.

Psychopharmacology (Berl) 2021 Jun 17. Epub 2021 Jun 17.

Department of Neurosurgery & Neurocritical Care, Huashan Hospital Affiliated To Fudan University, 958 Jinguang Road, Minhang District, Shanghai, 200040, China.

Background: Depression is a severe mental disorder. Unfortunately, more than half of patients with major depression disorder cannot achieve remission after initial treatment with an antidepressant. Geniposide, a bioactive iridoid glycoside isolated from Gardenia jasminoides Ellis, can ameliorate depressive-like behaviors in mice. However, the underlying mechanism is still not very clear.

Methods: The pharmacological methods including ELISA, immunofluorescence, and Western blot were used to investigate the role of geniposide on chronic unpredictable mild stress (CUMS)-induced depression mice.

Results: In this study, we found that geniposide could inhibit CUMS-induced depressive-like behaviors in mice. Geniposide is able to reduce the levels of ceramide and lower the activity of acid sphingomyelinase (ASM) in hippocampus; besides, ASM inhibitor (amitriptyline) can decrease the concentration of ceramide and ameliorate depressive-like behaviors of mice. Moreover, geniposide can also alleviate CUMS-induced hippocampal neuronal apoptosis and increase the phosphorylated form of PI3K, Akt, and GSK3β. Additionally, PI3K inhibitor (LY294002) can also abolish the neuroprotective effect of geniposide on hippocampal neurons in vitro.

Conclusions: These results indicate that geniposide exert a potential antidepressant-like effect on CUMS mice, and its effect might be associated with activated PI3K/Akt/GSK3β signaling, reduced the level of ceramide and hippocampal neuron apoptosis.
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http://dx.doi.org/10.1007/s00213-021-05895-8DOI Listing
June 2021

Deep learning for registration of region of interest in consecutive wireless capsule endoscopy frames.

Comput Methods Programs Biomed 2021 May 25;208:106189. Epub 2021 May 25.

College of Computer Science, Chongqing University, Chongqing, China.

Background And Objective: Functional gastrointestinal disorders (FGIDs) are reported as worldwide gastrointestinal (GI) diseases. GI motility assessment can assist the diagnosis of patients with intestine motility dysfunction. Wireless capsule endoscopy (WCE) can acquire images in the gastrointestinal (GI) tract including the small intestine where other conventional endoscopes cannot penetrate, and WCE images can reveal GI motility. To generally analyze WCE frames, the high-precision registration of consecutive WCE frames is an absolute necessity. It is difficult and meaningless to register entire WCE frames on a pixel level due to the unpredictable and massive non-rigid deformation between consecutive frames, the low quality of imaging and the complex intestinal environment. Thus, the registration of region of interest (ROI) functioning in a feature level has more significance than entire frame registration.

Methods: In this paper we present Timecylce-WCE, an end-to-end automatic registration approach of ROIs on WCE images. The clinicians can determine a ROI by drawing a bounding box in any WCE frame to be registered. This proposed approach is based on a deep-learning model of time-consistency in recurrent-registering, skip-registering and self-registering cycle, and it is fully unsupervised without any label. We incorporate the global correlation map with the local correlation map in matching the features, and a novel overall loss function is designed to enable the convergence of the model. As the output, a thin-plate spline (TPS) transformed region in the template frame is highly aligned with the query ROI in a finer-grained level. To the best of our knowledge this is the first time that a deep-learning-based registration method is proposed for WCE imaging motion.

Results: To highlight the effectiveness of the proposed approach, our proposed method is compared with the existing non-deep-learning methods and tested in a validation dataset with labeled matching points. The presented method resulted in the best [email protected] (Percentage of Correct Key-points, i.e., the predicted and the true joint is within the threshold - 10 pixels) of 66.49%. We also demonstrate that variants of design improved registration accuracy.

Conclusions: From the experimental analysis, it is clear that our proposed method outperforms the other existing methods. This lays the groundwork for subsequent studies, such as GI motility assessment, and WCE image synthesis.
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http://dx.doi.org/10.1016/j.cmpb.2021.106189DOI Listing
May 2021

Adiponectin modulates steroid hormone secretion, granulosa cell proliferation and apoptosis via binding its receptors during hens' high laying period.

Poult Sci 2021 Jul 11;100(7):101197. Epub 2021 Apr 11.

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China; Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou, 450046, China.

Adiponectin is an important adipocytokine and plays the roles in multiple metabolic processes via binding its receptors - AdipoR1 and AdipoR2, which has also been found to participate in the regulation of the reproductive system of animals, in particular by influencing the secretion of ovarian steroid hormones. To further investigate the expression of adiponectin and its receptors in follicles after in vitro incubation, and their role in the steroid synthesis of laying hens' ovaries, we performed qRT-PCR and ELISA to detect the expressions of AdipoQ, AdipoR1, and AidpoR2, and determined the key genes involved in steroidogenesis and the secretion of estradiol (E2) and progesterone (P4) through the in vitro activation of adiponectin (AipoRon) and overexpression or knockdown of AdipoR1 and AdipoR2. Our results revealed that adiponectin and its receptors wildly exist in follicles and granulosa cells, and AdipoRon (5 and 10 µg/mL) had no effect on granulosa cell proliferation and apoptosis but significantly stimulated the secretion of adiponectin and its receptors in granulosa cells after incubation for 24 h. Furthermore, AdipoRon could significantly stimulate the secretion of P4 and inhibit E2 level compared to those of the control group through modulating the key genes expression of steroidogenesis (CYP19A1, StAR, CYP11A1, FSHR, and LHR). The secretion of E2 was also decreased in granulosa cells by the treatments of overexpression and knockdown of AdipoR1/2, however, there was no difference in terms of the level of P4 and StAR expression between them if there was overexpression or knockdown of AdipoR1/2. In addition, it was shown that the secretion of E2 only exhibits a marked drop if co-processing 10 µg/mL AdipoRon and pGMLV AdipoR2 compared to single treatments. Taken together, the study highlighted the role of adiponectin and its receptors in the regulation of steroid synthesis and secretion in ovarian granulosa cells in laying hens.
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http://dx.doi.org/10.1016/j.psj.2021.101197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182267PMC
July 2021

Metabolite profiling, antioxidant and α-glucosidase inhibitory activities of buckwheat processed by solid-state fermentation with Eurotium cristatum YL-1.

Food Res Int 2021 05 5;143:110262. Epub 2021 Mar 5.

College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Province Key Laboratory of Food Science and Biotechnology, Changsha 410128, China. Electronic address:

Buckwheat was processed by solid-state fermentation (SSF) with the probiotic fungal strain Eurotium cristatum YL-1. The effects of SSF on the phytochemical content, as well as the antioxidant and α-glucosidase inhibitory activities, on buckwheat were revealed. Metabolite differences between non-fermented buckwheat (BW) and E. cristatum fermented buckwheat (FBW) were investigated by LC-MS/MS-based untargeted metabolomics. Results showed that 103 and 68 metabolites remarkably differed between BW and FBW in positive and negative ionization modes, respectively. Most phenolic compounds and alkaloids were significantly up-regulated during SSF. Hydrolytic enzymes (i.e., β-glucosidase, α-amylase, protease, and cellulase) were produced by the filamentous fungus E. cristatum during SSF. In vitro spectrophotometric assays demonstrated that the total phenolics content, ferric reducing antioxidant power, reducing power, scavenging activities of DPPH radical and ABTS, and α-glucosidase inhibitory activity of buckwheat were considerably enhanced after processing by SSF with E. cristatum. Additionally, solvents with different polarities significantly influenced the antioxidant and α-glucosidase inhibitory activities of buckwheat extracts. Our study indicated that processing by SSF with E. cristatum can greatly improve the phytochemical components of buckwheat and consequently contribute to its antioxidant and α-glucosidase inhibitory activities. SSF with E. cristatum is an innovative method for enhancing the health-promoting components and bioactivities of buckwheat.
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http://dx.doi.org/10.1016/j.foodres.2021.110262DOI Listing
May 2021

The EGFR Polymorphism Increased the Risk of Hepatocellular Carcinoma Through the miR-3196-Dependent Approach in Chinese Han Population.

Pharmgenomics Pers Med 2021 23;14:469-476. Epub 2021 Apr 23.

Department of Hepatobiliary and Pancreatic Surgery, LiYang People's Hospital, LiYang, 213300, Jiangsu, People's Republic of China.

Background: Previous studies have shown that epidermal growth factor receptor (EGFR) promotes cell proliferation through the PI3K-Akt-mTOR signaling pathway and participates in the occurrence and development of hepatocellular carcinoma (HCC). Here, we focused on the functional polymorphism of EGFR in the 3'-untranslated region (UTR), aiming to reveal the potential mechanisms by which functional polymorphism is associated with the risk and development of HCC in the Han Chinese population.

Methods: This study was a hospital-based case-control study. A total of 600 patients were enrolled, and another 600 healthy volunteers served as controls. The miR-associated SNPs in EGFR were screened, and genotyping was performed by TaqMan allele differential analysis. In this study, genotyping, real-time PCR, cell transfection and double luciferase reporter gene were used for subsequent analysis.

Results: HBV/HCV infection instead of alcohol exposure, smoking exposure, hypertension or diabetes mellitus was associated with an increased risk of HCC. Compared with TT genotypes, TG and GG genotypes of EGFR rs884225 were significantly associated with reduced HCC risk. The stratified analysis of association between rs884225 and HCC subgroup feature reveal a highly correlation with tumor size. Furthermore, qRT-PCR confirmed that EGFR rs884225, TG and GG genotypes were more likely to bind to miR-3196 and down-regulate EGFR level in cells, thereby inhibiting cell proliferation.

Conclusion: This study suggested that EGFR rs884225 is associated with a reduced risk of liver cancer and may be a developing biomarker.
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http://dx.doi.org/10.2147/PGPM.S304524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079348PMC
April 2021

State-of-the-Art and Opportunities for Forward Osmosis in Sewage Concentration and Wastewater Treatment.

Membranes (Basel) 2021 Apr 21;11(5). Epub 2021 Apr 21.

CSIRO Manufacturing, Clayton South, VIC 3169, Australia.

The application of membrane technologies for wastewater treatment to recover water and nutrients from different types of wastewater can be an effective strategy to mitigate the water shortage and provide resource recovery for sustainable development of industrialisation and urbanisation. Forward osmosis (FO), driven by the osmotic pressure difference between solutions divided by a semi-permeable membrane, has been recognised as a potential energy-efficient filtration process with a low tendency for fouling and a strong ability to filtrate highly polluted wastewater. The application of FO for wastewater treatment has received significant attention in research and attracted technological effort in recent years. In this review, we review the state-of-the-art application of FO technology for sewage concentration and wastewater treatment both as an independent treatment process and in combination with other treatment processes. We also provide an outlook of the future prospects and recommendations for the improvement of membrane performance, fouling control and system optimisation from the perspectives of membrane materials, operating condition optimisation, draw solution selection, and multiple technologies combination.
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http://dx.doi.org/10.3390/membranes11050305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143320PMC
April 2021

Astragaloside prevents UV induced keratinocyte injury by regulating TLR4/NF-κB pathway.

J Cosmet Dermatol 2021 Apr 24. Epub 2021 Apr 24.

Department of Dermatology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Pudong New District, Shanghai, 201399, China.

Background: Ultraviolet (UV) radiation is a key risk factor of environment to contribute photoaging and skin cancer through production of reactive oxygen species (ROS) and inflammatory responses. Here, we explored the effects of Astragaloside IV (AS-IV), an active component from Astragalus membranaceus, on UVB-induced oxidative injury and inflammatory response in human epidermal keratinocytes.

Methods: HaCaT keratinocytes were exposed to UVB irradiation, followed by AS-IV incubation. The cell viability, intracellular ROS level, oxidative stress, and apoptosis were determined. The regulatory effects of AS-IV on toll-like receptor 4 (TLR4) pathway in UVB-exposed HaCaT cells were also investigated.

Results: AS-IV pretreatment (10, 25, 50, 100 and 150 μM) increased cell viability in UVB-exposed HaCaT cells. AS-IV (50 μM) significantly reduced intracellular ROS level and lipid oxidation product malondialdehyde (MDA) content, and increased a ROS-scavenging enzyme superoxide dismutase (SOD) in HaCaT cells with UVB irradiation. In addition, AS-IV pretreatment suppressed apoptosis, increased Bax protein, caspase-3 and 9, and decreased BCL-2 protein in contrast to HaCaT cells with UVB-irradiation. AS-IV suppressed proinflammatory cytokine production, inhibited TLR4 and its downstream signaling molecules NF-κB, iNOS and cyclooxygenase-2 (COX-2) protein expression. We also found that the effects of AS-IV on cell viability and TLR4 expression was reversed by NAC. The protective of AS-IV on UVB-induced damage and TLR4 expression was dependent on ROS, as the increase in viability and decrease in TLR4 protein by AS-IV was significantly attenuated by ROS scavenger NAC (1 mM).

Conclusions: AS-IV prevent UVB-induced oxidative damage and inflammation by inhibiting TLR4 expression.
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http://dx.doi.org/10.1111/jocd.14174DOI Listing
April 2021

Structure and cytotoxicity of trichothecenes produced by the potato-associated fungus Trichothecium crotocinigenum.

Bioorg Chem 2021 Jun 5;111:104874. Epub 2021 Apr 5.

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, People's Republic of China; The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, People's Republic of China. Electronic address:

Seven previously undescribed trichothecenes, named trichothecrotocins M-S (1-7), along with five known compounds, were isolated from rice cultures of the potato-associated fungus Trichothecium crotocinigenum. Their structures and absolute configurations were determined through spectroscopic methods, single-crystal X-ray diffraction, and quantum chemistry calculations on ECD. Compound 1 possesses a rare 6,11-epoxy moiety in the trichothecene family. Compound 6 exhibited strong cytotoxic activity against MCF-7 cancer cell lines with an IC value of 2.34 ± 0.45 μM. It promoted apoptosis induction in MCF-7 cells. Moreover, cell cycle analysis showed cell cycle arrest caused by compound 6 at the G2/M phase which resulted to cell proliferation inhibition and pro-apoptotic activity. Further quantitative real-time PCR (qRT-PCR) analysis confirmed that the G2/M arrest was accompanied by upregulation of p21 and down regulation of cyclins B1 in 6-treated MCF-7 cells.
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http://dx.doi.org/10.1016/j.bioorg.2021.104874DOI Listing
June 2021

A cross-sectional seroepidemiology study of seven major enteroviruses causing HFMD in Guangdong, China.

J Infect 2021 Jul 16;83(1):119-145. Epub 2021 Apr 16.

National Institutes for Food and Drug Control, No.31, Huatuo Street, Beijing, P.R. China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2021.04.011DOI Listing
July 2021

Prenatal features of 17q12 microdeletion and microduplication syndromes: A retrospective case series.

Taiwan J Obstet Gynecol 2021 Mar;60(2):232-237

Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, Jiangsu, China. Electronic address:

Objective: To present the experience on prenatal features of 17q12 microdeletion and microduplication syndromes.

Materials And Methods: Prenatal chromosomal microarray analysis (CMA) were conducted between January 2015 and December 2018 at a single Chinese tertiary medical centre. Information of cases identified with 17q12 microdeletion or microduplication syndromes were retrospectively collected. Foetal ultrasonographic findings were reviewed, and other information about the gestation week at diagnosis, inheritance and pregnancy outcomes were also included.

Results: Ten pregnancies with 17q12 microdeletion and 4 with 17q12 microduplication were identified. The copy number variation (CNV) sizes were 1.39-1.94 Mb in the deleted cases and 1.42-1.48 Mb in the duplicated cases, respectively. All the duplicated and deleted regions included HNF1B and LHX1 genes. Most individuals with 17q12 deletion presented kidney anomalies (9/10), with renal hyperechogenicity being the most common finding (7/10). Fetuses with 17q12 duplication presented a wide phenotypic spectrum, including "double bubble" sign, structural anomalies of the heart and growth anomalies.

Conclusions: Our experience further demonstrated the high correlation between 17q12 microdeletion and renal anomalies especially hyperechogenic kidneys. Structural anomalies of the heart were newly identified phenotypes of 17q12 duplication during prenatal period. Besides, growth anomalies and duodenal atresia might be associated with the duplication.
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http://dx.doi.org/10.1016/j.tjog.2021.01.001DOI Listing
March 2021

Characterization of Molecular Species and Anti-Inflammatory Activity of Purified Phospholipids from Antarctic Krill Oil.

Mar Drugs 2021 Feb 25;19(3). Epub 2021 Feb 25.

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China.

The phospholipids (PLs) from Antarctic krill oil were purified (>97.2%) using adsorption column chromatography. Forty-nine PL molecular species were characterized by ultrahigh-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). Most of molecular species contained eicosapentaenoic acid (EPA, 20:5), docosahexaenoic acid (DHA, 22:6), docosapentaenoic acid (DPA, 22:5), and arachidonic acid (AA, 20:4). Notably, a special species PC (20:5/22:6) (1298.17 nmol/g) and many ether PLs were detected. The Antarctic krill PL liposome (IC = 0.108 mg/mL) showed better anti-inflammatory activity than crude Antarctic krill oil (IC = 0.446 mg/mL). It could block NF-κB signaling pathway via suppression of IκB-α degradation and p65 activation and dose-dependently reduce the cellular content of inflammatory mediators including nitric oxide (NO), reactive oxygen species (ROS), and inflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In addition, it can suppress carrageenan-induced mouse paw swelling. Results from the present study could provide a reference for better evaluation of nutritional and medicinal values of Antarctic krill oil.
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http://dx.doi.org/10.3390/md19030124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996531PMC
February 2021

Ultrasound-assisted closed reduction and percutaneous pinning for displaced and rotated lateral condylar humeral fractures in children.

J Shoulder Elbow Surg 2021 Feb 24. Epub 2021 Feb 24.

Department of Orthopedics Surgery, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China.

Background: It is difficult for surgeons to successfully perform closed reduction and percutaneous pinning on displaced and rotated lateral condylar humeral fractures in children. This study aimed to introduce an ultrasound-assisted closed reduction and percutaneous pinning technique and determine its usefulness in the treatment of displaced and rotated lateral condylar humeral fractures in children.

Methods: Between 2013 and 2018, 42 of 44 displaced and rotated pediatric lateral humeral condylar fractures were successfully treated with ultrasound-assisted closed reduction and percutaneous pinning. All surgical procedures were performed by 1 senior surgeon. Demographic and clinical data including age, sex, affected side, time from injury to reduction, operative time, and number of intraoperative radiographs (without fluoroscopy) were analyzed. Postoperative data were evaluated in terms of Kirschner wire in situ duration, follow-up duration, range of motion, carrying angle, cosmetic result, and complications.

Results: The average operative time was 67 minutes from 2013 to 2015 and 51 minutes from 2016 to 2018. All 42 patients who successfully underwent ultrasound-assisted closed reduction were followed up for >3 months, and 31 of 42 patients were followed up for >1 year. Among these 31 patients, the range-of-motion outcomes were excellent in 25 and good in 6. The carrying angle outcomes were excellent in 28 patients and good in 3. Two instances of wire infection, 9 instances of granulation tissue hyperplasia, and 23 instances of lateral spur formation occurred. No nonunion, avascular necrosis, or postoperative nerve issues were identified.

Conclusions: The surgical technique of ultrasound-assisted closed reduction and percutaneous pinning presented in this study can effectively help surgeons reduce displaced and rotated lateral condylar humeral fractures in children to avoid some open reductions and achieve satisfactory outcomes.
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http://dx.doi.org/10.1016/j.jse.2021.01.031DOI Listing
February 2021

GPR4 signaling is essential for the promotion of acid-mediated angiogenic capacity of endothelial progenitor cells by activating STAT3/VEGFA pathway in patients with coronary artery disease.

Stem Cell Res Ther 2021 02 25;12(1):149. Epub 2021 Feb 25.

Department of Cardiology, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, 519000, China.

Background: Patients with coronary artery disease (CAD) are characterized by a decline in vascular regeneration, which is related to the dysfunction of endothelial progenitor cells (EPCs). G-protein-coupled receptor 4 (GPR4) is a proton-sensing G-protein-coupled receptor (GPCR) that contributes to neovascularization in acidic microenvironments. However, the role of GPR4 in regulating the angiogenic capacity of EPCs from CAD patients in response to acidity generated in ischemic tissue remains completely unclear.

Methods: The angiogenic capacity of EPCs collected from CAD patients and healthy subjects was evaluated in different pH environments. The GPR4 function of regulating EPC-mediated angiogenesis was analyzed both in vitro and in vivo. The downstream mechanisms were further investigated by genetic overexpression and inhibition.

Results: Acidic environment prestimulation significantly enhanced the angiogenic capacity of EPCs from the non-CAD group both in vivo and in vitro, while the same treatment yielded the opposite result in the CAD group. Among the four canonical proton-sensing GPCRs, GPR4 displays the highest expression in EPCs. The expression of GRP4 was markedly lower in EPCs from CAD patients than in EPCs from non-CAD individuals independent of acid stimulation. The siRNA-mediated knockdown of GPR4 with subsequent decreased phosphorylation of STAT3 mimicked the impaired function of EPCs from CAD patients at pH 6.4 but not at pH 7.4. Elevating GPR4 expression restored the neovessel formation mediated by EPCs from CAD patients in an acidic environment by activating STAT3/VEGFA signaling. Moreover, the beneficial impact of GPR4 upregulation on EPC-mediated angiogenic capacity was abrogated by blockade of the STAT3/VEGFA signaling pathway.

Conclusions: Our present study demonstrated for the first time that loss of GPR4 is responsible for the decline in proton sensing and angiogenic capacity of EPCs from CAD patients. Augmentation of GPR4 expression promotes the neovessel formation of EPCs by activating STAT3/VEGF signaling. This finding implicates GPR4 as a potential therapeutic target for CAD characterized by impaired neovascularization in ischemic tissues.
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http://dx.doi.org/10.1186/s13287-021-02221-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905863PMC
February 2021

Surgical Selection of Unstable Intertrochanteric Fractures: PFNA Combined with or without Cerclage Cable.

Biomed Res Int 2021 8;2021:8875370. Epub 2021 Feb 8.

Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, China.

Due to the instability of unstable intertrochanteric fractures, the selection of a suitable internal fixation has always been a challenge for orthopedic surgeons. This study is aimed at comparing the clinical efficacy of PFNA combined with cerclage cable and without cerclage cable and finally recommend a stable internal fixation method to provide the basis for clinical therapy. From January 2014 to January 2018, we retrospectively analyzed all cases of unstable intertrochanteric fractures who received treatment in the Orthopedics Department of our hospital and finally screened 120 cases, 51 of whom were treated with cerclage cable, 69 without cerclage cable. The follow-up period was one year. HHS, BI, and RUSH scores were given within the specified time. We divided the patients into the PFNA+cable (PFNA combined with cerclage cable) group and the PFNA group. The time of fracture healing and weight-bearing in the PFNA+cable group was shorter than that in the PFNA group. With regard to HHS, BI, and RUSH, the PFNA+cable group was higher than the PFNA group at 1 month, 3 months, 6 months, and 12 months after operation. For HHS rating, the PFNA+cable group has a higher excellent rate than the PFNA group, which was 96.1% and 84.1%, respectively. All the results mentioned above were statistically significant. Compared with the group without cerclage cable, the application of cerclage cable can reduce the incidence of complications. From the comparison between the two groups, it can be seen that the surgical method of PFNA combined with cerclage cable can not only help to improve the stability of fracture reduction, shorten the time of fracture healing and postoperative weight-bearing, and significantly improve patients' self-care ability but also reduce the incidence of postoperative complications. Therefore, we think PFNA combined with cerclage cable is a good choice.
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http://dx.doi.org/10.1155/2021/8875370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884117PMC
May 2021

Correlation of placenta previa type with cesarean section blood loss and predictors of hysterectomy.

Chin Med J (Engl) 2021 Jan 27;134(4):501-502. Epub 2021 Jan 27.

Department of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated 6th People's Hospital, Shanghai Institute of Ultrasound in Medicine, Shanghai 200233, China.

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http://dx.doi.org/10.1097/CM9.0000000000001210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909324PMC
January 2021

Update: Innate Lymphoid Cells in Inflammatory Bowel Disease.

Dig Dis Sci 2021 Feb 20. Epub 2021 Feb 20.

Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Tongzipo Road, Changsha, 410013, Hunan, China.

Inflammatory bowel disease (IBD) is a chronic and nonspecific intestinal inflammatory condition with high relapse rate. Its pathogenesis has been linked to dysbacteriosis, genetic and environmental factors. In recent years, a new type of lymphocytes, termed innate lymphoid cells, has been described and classified into three subtypes of innate lymphoid cells-group 1, group 2 and group 3. An imbalance among these subsets' interaction with gut microbiome, and other immune cells affects intestinal mucosal homeostasis. Understanding the role of innate lymphoid cells may provide ideas for developing novel and targeted approaches for treatment of IBD.
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http://dx.doi.org/10.1007/s10620-021-06831-8DOI Listing
February 2021

Synergistic effects of Indian hedgehog and sonic hedgehog on chondrogenesis during cartilage repair.

J Mol Histol 2021 Apr 17;52(2):407-418. Epub 2021 Feb 17.

Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University, Shanghai, 200072, China.

Sonic hedgehog (Shh) and Indian hedgehog (Ihh) have been shown to control the induction of early cartilaginous differentiation. However, it is unclear whether Ihh and Shh exert synergistic effects on chondrogenesis during articular cartilage repair. Herein, we investigate the effects of chondrogenesis of bone-derived mesenchymal stem cells (BMSCs) following co-transfection with Shh and Ihh via adenoviral vectors in vitro and in vivo. A rotary cell culture system (RCCS) and Cytodex 3 microcarriers were used to create a stereoscopic dynamic environment for cell culture. In the RCCS environment, BMSCs co-transfected with Ihh and Shh displayed stronger chondrogenic differentiation and chondrogenesis than BMSCs transfected with Ihh or Shh alone, and exhibited higher expression levels of Sox 9, ACAN and collagen II, stronger toluidine blue and collagen II immunohistochemical staining. After transplanted into the osteochondral defect at 8 weeks, Ihh/Shh co-transfected BMSCs showed a significantly better cartilage repair than BMSCs transfected with Ihh or Shh alone. Ihh and Shh have synergistic effects on the induction of chondrogenic differentiation and chondrogenesis under a microgravity environment, and help to repair damaged cartilage and reverse subchondral defects during the early stages.
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http://dx.doi.org/10.1007/s10735-021-09964-2DOI Listing
April 2021

The etiology of achalasia: An immune-dominant disease.

J Dig Dis 2021 Mar;22(3):126-135

Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.

There is accumulating evidence suggesting that an autoimmune component is involved in esophageal achalasia. An increase in immune cells, cytokines, chemokines, and autoimmune antibodies in serum and infiltration of immune cells in tissues support the view that immune-mediated inflammation is a crucial pathogenesis of inhibitory neuron degeneration in the lower esophageal sphincter. Infection of viruses such as the herpes virus family has been suspected of provoking the autoimmune reaction. Meanwhile, previous reports on immunogenetics have proposed that specific risk alleles on the human leukocyte antigen complex define the susceptible population to achalasia. In this study we reviewed current knowledge regarding the immune-related factors of achalasia, including immunology, viral infection and immunogenetic variations.
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http://dx.doi.org/10.1111/1751-2980.12973DOI Listing
March 2021

Polydopamine-Assisted Two-Dimensional Molybdenum Disulfide (MoS)-Modified PES Tight Ultrafiltration Mixed-Matrix Membranes: Enhanced Dye Separation Performance.

Membranes (Basel) 2021 Jan 30;11(2). Epub 2021 Jan 30.

Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.

Tight ultrafiltration (TUF) membranes with high performance have attracted more and more attention in the separation of organic molecules. To improve membrane performance, some methods such as interface polymerization have been applied. However, these approaches have complex operation procedures. In this study, a polydopamine (PDA) modified MoS ([email protected]) blending polyethersulfone (PES) membrane with smaller pore size and excellent selectivity was fabricated by a simple phase inversion method. The molecular weight cut-off (MWCO) of as-prepared [email protected] mixed matrix membranes (MMMs) changes, and the effective separation of dye molecules in [email protected] MMMs with different concentrations were obtained. The addition amount of [email protected] increased from 0 to 4.5 wt %, resulting in a series of membranes with the MWCO values of 7402.29, 7007.89, 5803.58, 5589.50, 6632.77, and 6664.55 Da. The MWCO of the membrane M3 (3.0 wt %) was the lowest, the pore size was defined as 2.62 nm, and the pure water flux was 42.0 L m h bar. The rejection of Chromotrope 2B (C2B), Reactive Blue 4 (RB4), and Janus Green B (JGB) in aqueous solution with different concentrations of dyes was better than that of unmodified membrane. The separation effect of M3 and M0 on JGB at different pH values was also investigated. The rejection rate of M3 to JGB was higher than M0 at different pH ranges from 3 to 11. The rejection of M3 was 98.17-99.88%. When pH was 11, the rejection of membranes decreased with the extension of separation time. Specifically, at 180 min, the rejection of M0 and M3 dropped to 77.59% and 88.61%, respectively. In addition, the membrane had a very low retention of salt ions, Nacl 1.58%, NaSO 10.52%, MgSO 4.64%, and MgCl 1.55%, reflecting the potential for separating salts and dyes of [email protected]/PES MMMs.
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http://dx.doi.org/10.3390/membranes11020096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912618PMC
January 2021

End-Bonded Contacts of Tellurium Transistors.

ACS Appl Mater Interfaces 2021 Feb 5;13(6):7766-7772. Epub 2021 Feb 5.

State Key Laboratory of Infrared Physics, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, 500 Yutian Road, Shanghai 200083, China.

The development of novel low-dimensional materials makes the metallic contact to nanostructure facing challenges. Compared to side contacts, end-bonded contacts are proposed to be more effective pathways for charge injection and extraction. However, there is a lack of up-to-date understanding regarding end-bonded contacts, especially the recently emerged high-performance field-effect transistors (FETs). Here, the end-bonded contacts in tellurium (Te) transistors are first achieved by inducing metal semiconductor alloy. The formation of Pd-Te alloy structure is confirmed by a high-resolution transmission electron microscope (HRTEM) in Te-nanorod-based FETs. The ultralow specific contact resistance is estimated to be 5.1 × 10 Ω cm by the transmission line mode. On the basis of this finding, Te FETs are shown to exhibit incredible electronic properties, metal-insulator transition, and photodetection performance. This in-depth investigation of the end-bonded contact between Pd and Te speeds up the potential application of Te nanostructure and provides a feasible method for contact engineering in advanced devices.
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http://dx.doi.org/10.1021/acsami.0c21675DOI Listing
February 2021

Stabilization of Organic Cathodes by a Temperature-Induced Effect Enabling Higher Energy and Excellent Cyclability.

ACS Appl Mater Interfaces 2021 Feb 4;13(6):7178-7187. Epub 2021 Feb 4.

Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, Key Laboratory for Green Organic Synthesis and Application of Hunan Province, College of Chemistry, Xiangtan University, 411105 Xiangtan, China.

To face the challenge of all-climate application, organic rechargeable batteries must hold the capability of efficiently operating both at high temperatures (>50 °C) and low temperatures (-20 °C). However, the low electronic conductivity and high solubility of organic molecules significantly impede the development in electrochemical energy storage. This issue can be effectively diminished using functionalized porphyrin complex-based organic cathodes by the in-situ electropolymerization of electrodes at elevating temperatures during electrochemical cycling. [5,15-(ethynyl)-10,20-diphenylporphinato]copper(II) (CuDEPP)- and 5,15-(ethynyl)-10,20-diphenylporphinato (DEPP)-based cathodes are proposed as models, and it is proved that a largely improved electrochemical performance is observed in both cathodes at a high operating temperature. Reversible capacities of 249 and 105 mA h g are obtained for the CuDEPP and DEPP cathodes after 1000 cycles at 50 °C, respectively. The result indicates that the temperature-induced in situ electropolymerization strategy responds to the enhanced electrochemical performance. This study would open new opportunities for developing highly stable organic cathodes for electrochemical energy storage even at high temperatures.
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http://dx.doi.org/10.1021/acsami.0c20525DOI Listing
February 2021

Efficacy of silver needle therapy for the treatment of chronic nonspecific low back pain: a prospective, single-center, randomized, parallel-controlled clinical trial.

Trials 2021 Jan 21;22(1):75. Epub 2021 Jan 21.

Department of Pain Management, The Affiliated Hospital of Qingdao University, No. 1677, Wutai Mountain Road, Huangdao District, Qingdao City, 266000, Shan Dong Province, China.

Background: Chronic nonspecific low back pain (CNSLBP) troubles approximately 30% of people worldwide. Silver needle therapy (SNT) is a treatment method to relieve soft tissue pain through heating. Therefore, this study aimed to observe the effects of SNT on CNSLBP.

Methods: In this study, 100 patients were randomly divided into 2 groups: silver needle (SN) group and control group (n = 50). In the SN group, patients received SNT and physiotherapy, while patients received physiotherapy alone in the control group. At the 6-month follow-up, the numerical rating scale (NRS), Oswestry Disability Index (ODI), Short-Form 12 of quality of life (SF-12), the natural logarithms of low-frequency measurement (InLF), and the natural logarithms of high-frequency measurement (InHF) of heart rate variability (HRV) were recorded.

Results: In both groups, NRS, ODI, SF-12 scores, and HRV at 2 weeks after treatment were improved and maintained for 6 months. Compared with the control group, more significant improvements were observed in the NRS and SF-12 scores at 1, 2, 3, and 6 months and in the ODI scores at 1 and 2 months in the SN group (P <  0.05). However, there was no significant difference between the groups in the ODI scores at 3 and 6 months. InLF and InHF in the SN group were higher than those in the control group at 3 and 6 months (P <  0.05).

Conclusions: SNT relieved pain and improved quality of life and autonomic nerve activity, especially parasympathetic nerve, in patients with CNSLBP, without serious complications.

Trial Registration: Chinese Clinical Trial Registry No. ChiCTR-OOC-17013237 . Registered on November 11, 2017.
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http://dx.doi.org/10.1186/s13063-021-05040-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818943PMC
January 2021

The umbilical cord mesenchymal stem cell-derived exosomal lncRNA H19 improves osteochondral activity through miR-29b-3p/FoxO3 axis.

Clin Transl Med 2021 01;11(1):e255

Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, PR China.

Background: Our previous study revealed that the exosomal lncRNA H19 derived from umbilical cord mesenchymal stem cells (UMSCs) plays a pivotal role in osteochondral regeneration. In this study, we investigated whether the exosomal lncRNA H19 could act as a competing endogenous RNA (ceRNA) to potentiate osteochondral activity in chondrocytes.

Methods: Dual-luciferase reporter assay, RNA pull-down, RNA immunoprecipitation (RIP), and fluorescence in situ hybridization (FISH) were carried to verify the interaction between miR-29b-3p and both lncRNA H19 and the target mRNA FoxO3. Chondrocytes were treated with UMSC-derived exosomes, which highly expressing lncRNA H19 expression, followed by apoptosis, migration, senescence, and matrix secretion assessments. An in vivo SD rat cartilage defect model was carried out to explore the role and mechanism of lncRNA H19/miR-29b-3p.

Results: UMSCs were successfully identified, and exosomes were successfully extracted. Exosomes exhibited the ability to transfer lncRNA H19 to chondrocytes. Mechanistically, exosomal lncRNA H19 potentiated osteochondral activity by acting as a competing endogenous sponge of miR-29b-3p, and miR-29b-3p directly targeted FoxO3. Intra-articular injection of exosomes overexpressing lncRNA H19 could promote sustained cartilage repair; however, this effect could be undermined by miR-29b-3p agomir.

Conclusions: Our study revealed a significant role in the development of strategies against cartilage defects for UMSC-derived exosomes that overexpress lncRNA H19. Exosomal H19 was found to promote chondrocyte migration, matrix secretion, apoptosis suppression, as well as senescence suppression, both in vitro and in vivo. The specific mechanism lies in the fact that exosomal H19 acts as a ceRNA against miR-29b-3p to upregulate FoxO3 in chondrocytes.
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http://dx.doi.org/10.1002/ctm2.255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7805401PMC
January 2021

Characterization of the plastome of (Theaceae), an endangered and endemic yellow camellia species in China.

Mitochondrial DNA B Resour 2020 Oct 7;5(3):3527-3528. Epub 2020 Oct 7.

Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.

D. Fang is a shrub which is found on limestone of karst forests in Guangxi, China. In this study, we characterized the whole plastid genome of using Illumina paired-end sequencing reads. The plastome is 156,621 bp in length, containing two copies of inverted repeat (IR) regions (26,046 bp), a large-single copy (LSC) region (86,289 bp), and a small-single copy (SSC) region (18,240 bp). A total of 114 unique genes in the genome has 80 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. The phylogenetic result indicates is closely related to C. W. Chi.
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http://dx.doi.org/10.1080/23802359.2020.1828000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782419PMC
October 2020
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