Publications by authors named "Xing Wei"

731 Publications

A Novel Pretreatment Device Integrating Magnetic-Assisted Dispersive Extraction and Ultrasonic Spray Separation for Speciation Analysis of Arsenic in Whole Blood by Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry.

Anal Chem 2021 Jul 20. Epub 2021 Jul 20.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.

Speciation analysis of arsenic in blood is essential for identifying and quantifying the exposure of arsenic and studying the metabolism and toxicity of arsenic. Herein, a novel pretreatment device is rationally designed and used for speciation analysis of arsenic in whole blood by ion chromatography-inductively coupled plasma-mass spectrometry (IC-ICP-MS). The sample centrifuge tubes containing blood, reagents, and a magnetic stir bar are placed on the fidget spinner of the pretreatment device. When flicking the fidget spinner rotation with the finger, the magnetic stir bar in the tube rotates in three dimensions under the magnetic field, thereby assisting dispersive extraction of arsenic species by the mixing of blood with reagents. Afterward, the arsenic extract is separated in situ from the blood matrix using an ultrasonic spray sheet covered with a filter and ultrafiltration membrane, which is directly used for subsequent IC-ICP-MS analysis. For 100 μL of blood, the whole pretreatment operation can be completed within 10 min. With As(III), As(V), MMA, and DMA in blood as analytes, the use of the present pretreatment device will hardly lead to the loss and transformation of arsenic species, and the extraction efficiency of the total arsenic is more than 96%. When the pretreatment device is coupled to IC-ICP-MS, the detection limits of four arsenic species in whole blood are 0.017-0.023 μg L, and precisions are within 2.3-4.2%. This pretreatment device provides a simple, fast, efficient, and low-cost tool for extraction and separation of arsenic species in whole blood, opening a new idea for the pretreatment of complex samples.
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http://dx.doi.org/10.1021/acs.analchem.1c01745DOI Listing
July 2021

Evaluating the Histopathology of Pancreatic Ductal Adenocarcinoma by Intravoxel Incoherent Motion-Diffusion Weighted Imaging Comparing With Diffusion-Weighted Imaging.

Front Oncol 2021 23;11:670085. Epub 2021 Jun 23.

Department of Radiology, The Third Affiliated Hospital of Soochow University, Changzhou, China.

Objectives: To explore the differences between intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) and diffusion-weighted imaging (DWI) in evaluating the histopathological characters of pancreatic ductal adenocarcinoma (PDAC).

Methods: This retrospective study enrolled 50 patients with PDAC confirmed by pathology from December 2018 to May 2020. All patients underwent DWI and IVIM-DWI before surgeries. Patients were classified into low- and high-fibrosis groups. Apparent diffusion coefficient (ADC), diffusion coefficient (D), false diffusion coefficient (D*), and perfusion fraction (f) were measured by two radiologists, respectively in GE AW 4.7 post-processing station, wherein ADC values were derived by mono-exponential fits and f, D, D* values were derived by biexponential fits. The tumor tissue was stained with Sirius red, CD34, and CK19 to evaluate fibrosis, microvascular density (MVD), and tumor cell density. Furthermore, the correlation between ADC, D, D*, and f values and histopathological results was analyzed.

Results: The D values were lower in the high-fibrosis group than in the low-fibrosis group, while the f values were opposite. Further, no statistically significant differences were detected in ADC and D* values between the high- and low-fibrosis groups. The AUC of D and f values had higher evaluation efficacy in the high- and low-fibrosis groups than ADC values. A significant negative correlation was established between D values, and fibrosis and a significant positive correlation were observed between f values and fibrosis. No statistical difference was detected between DWI/IVIM parameters values and MVD or tumor cell density except for the positive correlation between D* values and tumor cell density.

Conclusions: D and f values derived from the IVIM model had higher sensitivity and diagnostic performance for grading fibrosis in PDAC compared to the conventional DWI model. IVIM-DWI may have the potential as an imaging biomarker for predicting the fibrosis grade of PDAC.
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http://dx.doi.org/10.3389/fonc.2021.670085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261286PMC
June 2021

A Genetic-Algorithm-Based Optimization Routing for FANETs.

Front Neurorobot 2021 15;15:697624. Epub 2021 Jun 15.

School of Computer Science and Information Technology, Guangxi Normal University, Guilin, China.

In view of the characteristics of high mobility of FANETs nodes, combined with the features of Topology-based class routing protocol on-demand search, a Genetic-algorithm-based routing (GAR) protocol is proposed for FANETs which based on improved genetic algorithm for FANETs route search, and it taking into account the link stability, link bandwidth, node energy, and other factors. GAR improves the selection, crossover, and variation operators of the genetic algorithm so that GAR can finally plan an optimized route from the communication initiating node to the destination node quickly using a smaller cost. The experimental results show that GAR can largely improve the throughput, reduce the delay and improve the stability of the network, which is more suitable for FANETs.
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http://dx.doi.org/10.3389/fnbot.2021.697624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239347PMC
June 2021

InfPolyn, a Nonparametric Bayesian Characterization for Composition-Dependent Interdiffusion Coefficients.

Materials (Basel) 2021 Jun 29;14(13). Epub 2021 Jun 29.

School of Integrated Circuit Science and Engineering, Beihang University, Beijing 100191, China.

Composition-dependent interdiffusion coefficients are key parameters in many physical processes. However, finding such coefficients for a system with few components is challenging due to the underdetermination of the governing diffusion equations, the lack of data in practice, and the unknown parametric form of the interdiffusion coefficients. In this work, we propose InfPolyn, Infinite Polynomial, a novel statistical framework to characterize the component-dependent interdiffusion coefficients. Our model is a generalization of the commonly used polynomial fitting method with extended model capacity and flexibility and it is combined with the numerical inversion-based Boltzmann-Matano method for the interdiffusion coefficient estimations. We assess InfPolyn on ternary and quaternary systems with predefined polynomial, exponential, and sinusoidal interdiffusion coefficients. The experiments show that InfPolyn outperforms the competitors, the SOTA numerical inversion-based Boltzmann-Matano methods, with a large margin in terms of relative error (10× more accurate). Its performance is also consistent and stable, whereas the number of samples required remains small.
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http://dx.doi.org/10.3390/ma14133635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269731PMC
June 2021

Nano-Mechanical Properties and Creep Behavior of Ti6Al4V Fabricated by Powder Bed Fusion Electron Beam Additive Manufacturing.

Materials (Basel) 2021 Jun 1;14(11). Epub 2021 Jun 1.

School of Mechanical and Automotive Engineering, South China University of Technology, Guangzhou 510640, China.

Effects of scanning strategy during powder bed fusion electron beam additive manufacturing (PBF-EB AM) on microstructure, nano-mechanical properties, and creep behavior of Ti6Al4V alloys were compared. Results show that PBF-EB AM Ti6Al4V alloy with linear scanning without rotation strategy was composed of 96.9% α-Ti and 2.7% β-Ti, and has a nanoindentation range of 4.11-6.31 GPa with the strain rate ranging from 0.001 to 1 s, and possesses a strain-rate sensitivity exponent of 0.053 ± 0.014. While PBF-EB AM Ti6Al4V alloy with linear and 90° rotate scanning strategy was composed of 98.1% α-Ti and 1.9% β-Ti and has a nanoindentation range of 3.98-5.52 GPa with the strain rate ranging from 0.001 to 1 s, and possesses a strain-rate sensitivity exponent of 0.047 ± 0.009. The nanohardness increased with increasing strain rate, and creep displacement increased with the increasing maximum holding loads. The creep behavior was mainly dominated by dislocation motion during deformation induced by the indenter. The PBF-EB AM Ti6Al4V alloy with only the linear scanning strategy has a higher nanohardness and better creep resistance properties than the alloy with linear scanning and 90° rotation strategy. These results could contribute to understanding the creep behavior of Ti6Al4V alloy and are significant for PBF-EB AM of Ti6Al4V and other alloys.
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http://dx.doi.org/10.3390/ma14113004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199181PMC
June 2021

Fetal growth standards for Chinese twin pregnancies.

BMC Pregnancy Childbirth 2021 Jun 22;21(1):436. Epub 2021 Jun 22.

Department of Fetal Medicine and Prenatal Diagnosis Center, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, 2699 West Gaoke Rd, Shanghai, 201204, China.

Background: The common use of singleton fetal growth standard to access twin growth might lead to over-monitoring and treatment. We aimed to develop fetal growth standards for Chinese twins based on ultrasound measurements, and compare it with Zhang's and other twin fetal growth charts.

Methods: A cohort of uncomplicated twin pregnancies were prospectively followed in 2014-2017. Smoothed estimates of fetal growth percentiles for both monochorionic (MC) and dichorionic (DC) twins were obtained using a linear mixed model. We also created growth charts for twins using a model-based approach proposed by Zhang et al. Our twin standards were compared with Hadlock's (singleton) in predicting adverse perinatal outcomes.

Results: A total of 398 twin pregnancies were included, with 214 MC and 582 DC live-born twins. The MC twins were slightly lighter than the DC twins, with small differences throughout the gestation. Our ultrasound-based fetal weight standards were comparable to that using Zhang's method. Compared with previous references/standards from the US, Brazil, Italy and UK, our twins had very similar 50th percentiles, but narrower ranges between the 5th and 95th or 10th and 90th percentiles. Compared with the Hadlock's standard, the risks of neonatal death and adverse perinatal outcomes for small for gestational age (SGA) versus non-SGA were substantially elevated using our standards.

Conclusions: A normal fetal growth standard for Chinese twins was created. The differences between MC and DC twins were clinically insignificant. The 50th weight percentiles of the Chinese twins were identical to those in other races/ethnicities but the ranges were markedly narrower. Our standard performed much better than the Hadlock's in predicting low birth weight infants associated with adverse perinatal outcomes in twin pregnancies. The present study also indicated that Zhang's method is applicable to Chinese twins, and other areas may use Zhang's method to generate their own curves for twins if deemed necessary.
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http://dx.doi.org/10.1186/s12884-021-03926-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220745PMC
June 2021

The discrepancy of aromatase expression in epicardial adipose tissue between CHD and non-CHD patients.

Cardiovasc J Afr 2021 Jun 7;32:1-4. Epub 2021 Jun 7.

Department of Cardiovascular Surgery, Shanxi Cardiovascular Hospital, Taiyuan, PR of China. Email:

Objectives: Epicardial adipose tissue (EAT) aromatase converts androstenedione and other adrenal androgens into oestrogens. The locally produced oestradiol (E) may have cardiovascular protective effects. Little is known about the relationship between EAT aromatase level and coronary heart disease (CHD). Here, we compared EAT aromatase levels in CHD versus non-CHD patients and assessed the relationship between EAT aromatase levels and lesion degree in the coronary arteries.

Methods: EAT and blood specimens were obtained from patients undergoing thoracotomy prior to cardiopulmonary bypass. Serum E levels were obtained from our hospital laboratory. EAT aromatase expression was determined by RT-qPCR and ELISA assays. All patients underwent coronary angiography and the level of coronary lesions was evaluated with the SYNTAX score.

Results: Compared with non-CHD patients, CHD patients had lower EAT aromatase mRNA and protein levels. In the CHD patients, EAT aromatase and oestrogen levels negatively correlated with the severity of coronary artery disease.

Conclusions: Our data revealed that reduced EAT aromatase levels correlated with coronary atherosclerotic lesions. Reduced EAT aromatase protein levels may aggravate the severity of atherosclerosis. Future studies should investigate the mechanisms regulating aromatase expression in epicardial fat.
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http://dx.doi.org/10.5830/CVJA-2021-012DOI Listing
June 2021

Comparison of Chief Complaints and Patient-Reported Symptoms of Treatment-Naive Lung Cancer Patients Before Surgery.

Patient Prefer Adherence 2021 25;15:1101-1106. Epub 2021 May 25.

Center for Cancer Prevention Research, Sichuan Cancer Hospital & Institute, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, People's Republic of China.

Background: Lung cancer patients without chief complaints have been increasingly identified by physical examination. This study aimed to profile and compare chief complaints with patient-reported symptoms of lung cancer patients before surgery.

Methods: Data were extracted from a multicenter, prospective longitudinal study (CN-PRO-Lung 1) in China from November 2017 to January 2020. A comparison between chief complaints and patient-reported symptoms was analyzed using the Chi-squared test.

Results: A total of 201 (50.8%) lung cancer patients without chief complaints were found by physical examination at admission, and 195 (49.2%) patients had chief complaints. The top 5 chief complaints were coughing (38.1%), expectoration (25.5%), chest pain (13.6%), hemoptysis (10.6%), and shortness of breath (5.3%). There were significantly more patients with chief complaints of coughing (38.1% vs 15.0%, P <0.001) and pain (20.5% vs 6.9%, P<0.001) than those with the same symptoms rated ≥4 via MD Anderson Symptom Inventory‒Lung Cancer (MDASI-LC). There were less patients with chief complaints of fatigue (1.8% vs 10.9%, P<0.001), nausea (0.3% vs 2.5%, P=0.006), and vomiting (0.3% vs 1.8%, p=0.032) than those with the same symptoms rated ≥4 via MDASI-LC. In patients without chief complaints, the five most common moderate to severe patient-reported symptoms were disturbed sleep (19.5%), distress (13.5%), dry mouth (13%), sadness (12%), and difficulty remembering (11.1%).

Conclusion: Symptoms of lung cancer patients not included in the chief complaint could be identified via a patient-reported outcome instrument, suggesting the necessity of implementing the patient-reported outcome assessment before lung cancer surgery for better patient care.
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http://dx.doi.org/10.2147/PPA.S307814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164718PMC
May 2021

Two-Dimensional Cytometry Platform for Single-Particle/Cell Analysis with Laser-Induced Fluorescence and ICP-MS.

Anal Chem 2021 06 2;93(23):8203-8209. Epub 2021 Jun 2.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China.

A two-dimensional cytometry platform (CytoLM) with high sensitivity and high temporal resolution is developed for single-particle and single-cell sampling and analysis. First, a flow-assisted vortex capillary cell sampling (VCCS) unit confines the sample stream in curved flow and drives to focus and align the particles or cells in a small probe volume. By coupling VCCS to a laser-induced fluorescence (LIF) detector with data acquisition and processing capability, a high-throughput single-particle/cell analysis system (VCCS-LIF) was established. The particle analysis throughput of 119.42/s and a detection recovery of 78.20 ± 1.75% were achieved at a density of 9.16 × 10/mL for fluorescent particles, and the cell analysis throughput is 48.20/s at a density of 1.5 × 10/mL. Second, the CytoLM platform is constructed by hyphenating VCCS-LIF with inductively coupled plasma mass spectrometry (ICP-MS). In the analysis of HepG2 cells by Ag incubation and AO staining, 10,760 fluorescence bursts and 3068 MS events were observed in 240 s. Invalid signals due to undispersed cells were controlled at 3.80% for LIF and 1.01% for MS, with a proportion of effective signal of >96.20%. After peak identification and integral processing of the original data, the statistical results including peak area, height, width, and spacing are obtained concurrently and the information on concentration and elemental quantification of single cells is evaluated. CytoLM facilitates high-throughput, multi-dimensional, and multi-parameter characterization of particles and cells, and it may provide vast potential in life science analysis.
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http://dx.doi.org/10.1021/acs.analchem.1c00484DOI Listing
June 2021

Identification of Hub Genes to Regulate Breast Cancer Spinal Metastases by Bioinformatics Analyses.

Comput Math Methods Med 2021 12;2021:5548918. Epub 2021 May 12.

Department of Pathology, Inner Mongolia People's Hospital, Hohhot, 010017 Inner Mongolia, China.

Breast cancer (BC) had been one of the deadliest types of cancers in women worldwide. More than 65% of advanced-stage BC patients were identified to have bone metastasis. However, the molecular mechanisms involved in the BC spinal metastases remained largely unclear. This study screened dysregulated genes in the progression of BC spinal metastases by analyzing GSE22358. Moreover, we constructed PPI networks to identify key regulators in this progression. Bioinformatics analysis showed that these key regulators were involved in regulating the metabolic process, cell proliferation, Toll-like receptor and RIG-I-like receptor signaling, and mRNA surveillance. Furthermore, our analysis revealed that key regulators, including C1QB, CEP55, HIST1H2BO, IFI6, KIAA0101, PBK, SPAG5, SPP1, DCN, FZD7, KRT5, and TGFBR3, were correlated to the OS time in BC patients. In addition, we analyzed TCGA database to further confirm the expression levels of these hub genes in breast cancer. Our results showed that these regulators were significantly differentially expressed in breast cancer, which were consistent with GSE22358 dataset analysis. Furthermore, our analysis demonstrated that CEP55 was remarkably upregulated in the advanced stage of breast cancer compared to the stage I breast cancer sample and was significantly upregulated in triple-negative breast cancers (TNBC) compared to other types of breast cancers, including luminal and HER2-positive cancers, demonstrating CEP55 may have a regulatory role in TNBC. Finally, our results showed that CEP55 was the most highly expressed in Basal-like 1 TNBC and Basal-like 2 TNBC samples but the most lowly expressed in mesenchymal stem-like TNBC samples. Although more studies are still needed to understand the functions of key regulators in BC, this study provides useful information to understand the mechanisms underlying BC spinal metastases.
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http://dx.doi.org/10.1155/2021/5548918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133842PMC
May 2021

Retraction Note to: Decellularized scaffolds containing hyaluronic acid and EGF for promoting the recovery of skin wounds.

J Mater Sci Mater Med 2021 May 26;32(6):62. Epub 2021 May 26.

Key Lab for Genetic Medicine of Guangdong Province, National Engineering Research Center of Genetic Medicine, Institute of Biomedicine, Jinan University, Guangzhou, 510632,, Guangdong, China.

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http://dx.doi.org/10.1007/s10856-021-06531-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154757PMC
May 2021

Preoperative prediction for lauren type of gastric cancer: A radiomics nomogram analysis based on CT images and clinical features.

J Xray Sci Technol 2021 May 21. Epub 2021 May 21.

Department of Radiology, Affiliated Hospital of Jiangnan University, Wuxi City, Jiangsu, China.

Purpose: To investigate feasibility of predicting Lauren type of gastric cancer based on CT radiomics nomogram before operation.

Materials And Methods: The clinical data and pre-treatment CT images of 300 gastric cancer patients with Lauren intestinal or diffuse type confirmed by postoperative pathology were retrospectively analyzed, who were randomly divided into training set and testing set with a ratio of 2:1. Clinical features were compared between the two Lauren types in the training set and testing set, respectively. Gastric tumors on CT images were manually segmented using ITK-SNAP software, and radiomic features of the segmented tumors were extracted, filtered and minimized using the least absolute shrinkage and selection operator (LASSO) regression to select optimal features and develop radiomics signature. A nomogram was constructed with radiomic features and clinical characteristics to predict Lauren type of gastric cancer. Clinical model, radiomics signature model, and the nomogram model were compared using the receiver operating characteristic (ROC) curve analysis with area under the curve (AUC). The calibration curve was used to test the agreement between prediction probability and actual clinical findings, and the decision curve was performed to assess the clinical usage of the nomogram model.

Results: In clinical features, Lauren type of gastric cancer relate to age and CT-N stage of patients (all p <  0.05). Radiomics signature was developed with the retained 10 radiomic features. The nomogram was constructed with the 2 clinical features and radiomics signature. Among 3 prediction models, performance of the nomogram was the best in predicting Lauren type of gastric cancer, with the respective AUC, accuracy, sensitivity and specificity of 0.864, 78.0%, 90.0%, 70.0%in the testing set. In addition, the calibration curve showed a good agreement between prediction probability and actual clinical findings (p >  0.05).

Conclusion: The nomogram combining radiomics signature and clinical features is a useful tool with the increased value to predict Lauren type of gastric cancer.
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http://dx.doi.org/10.3233/XST-210888DOI Listing
May 2021

Intracellular silver speciation by coupling capillary electrophoresis to ICP-MS integrating a high performance spiral flow spray chamber.

Anal Chim Acta 2021 Jun 29;1166:338540. Epub 2021 Apr 29.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China. Electronic address:

The study of silver species and their distribution/transformation in cell interior is of high significance for the elucidation of toxicology of silver nanoparticles (AgNPs). The intracellular speciation of dissolved Ag(I) and AgNPs was reported. The analytical platform integrated capillary electrophoresis (CE) to inductively coupled plasma-mass spectrometry (ICP-MS) incorporating a high efficiency interface and a high performance spiral flow spray chamber (SFSC). The interface and the SFSC provide a favorable detection limit of 87 ng L for the dissolved Ag(I). Total silver content was quantified by ICP-MS subject to digestion of the cell lysate, and quantification of AgNPs was carried out by subtraction. The speciation of dissolved Ag(I) and AgNPs in culture medium and HepG2 cells was performed, with RSDs of <3% for relative peak area and <2% for migration time, as well as spiking recoveries of 93.8%-94.3% in opti-MEM and 92.7%-106.6% in cell lysate. The present study indicated higher solubility of AgNPs in the cell interior with respect to that in the culture medium, due to oxidative stress or acidic microenvironment in the cancer cells.
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http://dx.doi.org/10.1016/j.aca.2021.338540DOI Listing
June 2021

Delayed brain development of Rolandic epilepsy profiled by deep learning-based neuroanatomic imaging.

Eur Radiol 2021 May 20. Epub 2021 May 20.

Department of Medical Imaging, Jinling Hospital, the First School of Clinical Medicine, Southern Medical University, Nanjing, China.

Objectives: Although Rolandic epilepsy (RE) has been regarded as a brain developmental disorder, neuroimaging studies have not yet ascertained whether RE has brain developmental delay. This study employed deep learning-based neuroanatomic biomarker to measure the changed feature of "brain age" in RE.

Methods: The study constructed a 3D-CNN brain age prediction model through 1155 cases of typically developing children's morphometric brain MRI from open-source datasets and further applied to a local dataset of 167 RE patients and 107 typically developing children. The brain-predicted age difference was measured to quantitatively estimate brain age changes in RE and further investigated the relevancies with cognitive and clinical variables.

Results: The brain age estimation network model presented a good performance for brain age prediction in typically developing children. The children with RE showed a 0.45-year delay of brain age by contrast with typically developing children. Delayed brain age was associated with neuroanatomic changes in the Rolandic regions and also associated with cognitive dysfunction of attention.

Conclusion: This study provided neuroimaging evidence to support the notion that RE has delayed brain development.

Key Points: • The children with Rolandic epilepsy showed imaging phenotypes of delayed brain development with increased GM volume and decreased WM volume in the Rolandic regions. • The children with Rolandic epilepsy had a 0.45-year delay of brain-predicted age by comparing with typically developing children, using 3D-CNN-based brain age prediction model. • The delayed brain age was associated with morphometric changes in the Rolandic regions and attentional deficit in Rolandic epilepsy.
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http://dx.doi.org/10.1007/s00330-021-08048-9DOI Listing
May 2021

Synergistic regulation of methylation and SP1 on MAGE-D4 transcription in glioma.

Am J Transl Res 2021 15;13(4):2241-2255. Epub 2021 Apr 15.

Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University Nanning, Guangxi, P. R. China.

Background: The family of MAGE genes is well known due to the majority of MAGE genes expressing specifically in tumor tissues while restrictedly in normal tissues. MAGE-D4 is one of the MAGE family and considered as a promising target for glioma immunotherapy because of its overexpression in glioma and restricted expression in normal tissues. Whereas the mechanism of MAGE-D4 heterogeneous expression in glioma has not yet been elucidated. In this study, the transcriptional regulation mechanism of MAGE-D4 in glioma is focused from the perspectives of promoter methylation and SP1.

Methods: Dual-luciferase reporter assay was performed to identify the core promoter of MAGE-D4 gene. Mass spectrometry was applied to quantify the methylation status of MAGE-D4 promoter in 50 glioma and 9 normal brain tissues. The influence of methylation and SP1 on MAGE-D4 transcriptional activity was evaluated by dual-luciferase reporter assay, qRT-PCR, western blot and ChIP-qPCR. Decitabine, an epigenetic drug, was used to treat the glioma cells. Then the treated cells were evaluated the influence of demethylation on SP1 binding to MAGE-D4 promoter.

Results: The -358 to +172 bp region was identified as the core promoter of MAGE-D4 gene which demonstrated hypomethylated and negative correlation between methylation level and MAGE-D4 mRNA expression in glioma tissues. For single CpG unit analysis, 8 CpG units (CpG unit 1, 2, 3, 4, 5, 6, 9 and 12) in MAGE-D4 core promoter showed hypomethylated in glioma and the methylation level of CpG unit 6 was positively associated with the prognosis of glioma patients. Furthermore, the methylation level of CpG unit 1 and 6 was negative negatively correlated with MAGE-D4 mRNA expression. Then, the results demonstrated that the promoter activity of MAGE-D4 was decreased by methylation in glioma cell lines. In addition, SP1 can binds directly to the MAGE-D4 promoter leading to up-regulation of MAGE-D4 mRNA through activation of its promoter. Finally, demethylation of MAGE-D4 promoter could benefit the SP1 binding and resulting co-activation of MAGE-D4 promoter by demethylation and SP1 in glioma cell lines.

Conclusion: These findings indicate that the synergies of promoter hypomethylation and SP1 up-regulated MAGE-D4 transcription in glioma, which implies a potential approach to resolve the heterogeneous expression of MAGE-D4 in order to establish foundation for the MAGE-D4 based glioma therapy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129322PMC
April 2021

Clinical characterization and the improved molecular diagnosis of autosomal dominant cone-rod dystrophy in patients with SCA7.

Mol Vis 2021 7;27:221-232. Epub 2021 May 7.

Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Purpose: To evaluate the retinal phenotype and genetic features of Chinese patients with spinocerebellar ataxia type 7 (SCA7).

Methods: Detailed ophthalmic examinations, including electroretinograms, fundus photography, fundus autofluorescence and optical coherence tomography, were performed to analyse the retinal lesions of patients with SCA7. A molecular genetic analysis was completed to confirm the number of CAG repeats in gene on the patients and their family members.

Results: Eight patients from three families with SCA7 were included in this study. Trinucleotide repeat was expanded from 43 to 113 in the affected patients. The affected patients were characterized by different degrees of cone-rod dystrophy, which is positively related to the number of CAG repeats and age. All patients complained of progressive bilateral visual loss, and most cases reported visual disturbance earlier than gait movement or dysarthria. A coarse granular appearance of the macular region on scanning laser ophthalmoscopy, hypofluorescence in the macula on autofluorescence, retinal atrophy on optic coherence tomography, depression of multifocal electroretinograms and prominent abnormalities in cone-mediated responses on electrograms are the general features of SCA7-related retinopathy. Hyperreflective dots in the outer retinal layers and choroidal vessel layers are a common sign in optic coherence tomography in the advanced stage.

Conclusions: SCA7 shows a cone-rod dystrophy phenotype. The multimodal imaging of the retina is beneficial to detect the early lesions of cone-rod dystrophy related to SCA7.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116265PMC
May 2021

ASO Author Reflections: Using Patient-Reported Outcomes to Evaluate Surgical Approaches for Locally Advanced Lung Cancer.

Ann Surg Oncol 2021 May 15. Epub 2021 May 15.

School of Public Health and Management, Chongqing Medical University, Chongqing, China.

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http://dx.doi.org/10.1245/s10434-021-10018-wDOI Listing
May 2021

Application of in situ simulation teaching in the training of trainee nurses to respond to emergencies.

Ann Palliat Med 2021 Apr;10(4):4509-4515

Department of General surgery, Tunchang People's Hospital, Tunchang, China.

Background: To explore the effect of the in situ simulation teaching method in the emergency training of trainee nurses.

Methods: A total of 108 trainee nurses from the First Affiliated Hospital of Hainan Medical College were selected, and in situ simulation teaching was employed in emergency training. Following the in situ simulation teaching training, a questionnaire was issued to evaluate CIPP (context evaluation, input evaluation, process evaluation, product evaluation) simulation teaching, clinical thinking ability, nursing team cooperation, and student satisfaction. These data were then collected and statistical analysis was conducted.

Results: The response rate of this teaching questionnaire was 100%. After using in situ simulation teaching to instruct trainee nurses in emergencies, the satisfaction rate of the trainee nurses was 94.9%, and the satisfaction rate of the instructor with the trainee nurses was 92.2%. After in-situ simulation teaching, the clinical thinking ability (critical thinking ability, systematic thinking ability, evidence-based thinking ability), teamwork ability (trust, team orientation, support, shared mental model and team leadership), the theoretical and clinical practice ability had been improved.

Conclusions: Most nurses agreed that the in situ simulation teaching method can cultivate clinical thinking and teamwork ability for common emergencies, thereby improving their comprehensive quality and job competence, which is invaluable when responding to emergencies.
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http://dx.doi.org/10.21037/apm-21-545DOI Listing
April 2021

Value of Exome Sequencing in Diagnosis and Management of Recurrent Non-immune Hydrops Fetalis: A Retrospective Analysis.

Front Genet 2021 9;12:616392. Epub 2021 Apr 9.

Department of Fetal Medicine, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.

The purpose of the study was to use exome sequencing (ES) to study the contribution of single-gene disorders to recurrent non-immune hydrops fetalis (NIHF) and retrospectively evaluate the value of genetic diagnosis on prenatal management and pregnancy outcome. From January 2012 to October 2018, a cohort of 28 fetuses with recurrent NIHF was analyzed by trio ES. Fetuses with immune hydrops, non-genetic factors (including infection, etc.), karyotype, or CNV abnormalities were excluded. Variants were interpreted based on ACMG/AMP guidelines. Fetal therapy was performed on seven fetuses. Of the 28 fetuses, 10 (36%) were found to carry causal genetic variants (pathogenic or likely pathogenic) in eight genes (, , , , , , , and ). Five (18%) fetuses had variant(s) of uncertain significance (VUS). Of the 10 fetuses with definitive molecular diagnosis, five (50%) were diagnosed with inborn errors of metabolism. Among the seven fetuses who received fetal therapy, two had definitive molecular diagnosis and resulted in neonatal death. Among the remaining five fetuses with negative results, four had newborn survival and one had intrauterine fetal death. Trio ES could facilitate genetic diagnosis of recurrent NIHF and improve the prenatal management and pregnancy outcome.
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http://dx.doi.org/10.3389/fgene.2021.616392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063045PMC
April 2021

Patient-Reported Outcomes of Video-Assisted Thoracoscopic Surgery Versus Thoracotomy for Locally Advanced Lung Cancer: A Longitudinal Cohort Study.

Ann Surg Oncol 2021 Apr 20. Epub 2021 Apr 20.

School of Public Health and Management, Chongqing Medical University, Chongqing, China.

Background: The effects of video-assisted thoracoscopic surgery (VATS) and traditional thoracotomy with respect to patient-reported outcomes (PROs) have only been assessed for early-stage lung cancer. This study was a longitudinal PRO assessment to compare the effects of these surgeries for locally advanced (stage II and III) lung cancer from the patients' perspective.

Methods: We investigated lung cancer patients from a previous prospective, multicentre study. Longitudinal data of clinical characteristics and PROs were collected. PROs were obtained preoperatively, daily in the hospital postoperatively, and weekly up to 4 weeks after discharge or the beginning of postoperative adjuvant therapy. Symptoms and impact on daily functioning and quality of life (QOL) were assessed by using the MD Anderson Symptom Inventory for lung cancer and a single-item QOL scale. Trajectories of PROs over the investigation period were compared.

Results: Overall, 117 primary lung cancer patients (stage II or III), including 63 and 54 patients who underwent VATS and traditional thoracotomy, respectively, were included. During postoperative hospitalization, VATS patients reported milder disturbed sleep (p = 0.048), drowsiness (p = 0.008), and interference with activity (p = 0.001), as well as better work ability (p < 0.0001), walking ability (p < 0.0001), and life enjoyment (p = 0.004). Post-discharge, VATS patients had less distress (p = 0.039), milder pain (p = 0.006), better work ability (p = 0.001), and better QOL (p = 0.047).

Conclusions: Locally advanced lung cancer patients who underwent VATS had lower postoperative symptom burden, less daily function interference, and better QOL than those who underwent thoracotomy.
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http://dx.doi.org/10.1245/s10434-021-09981-1DOI Listing
April 2021

Evaluation of the anti-inflammatory and antioxidant pharmcodynamic compoents of naoxintong capsules as a basis of broad spectrum effects.

Pharm Biol 2021 Dec;59(1):242-251

State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Context: Naoxintong capsule (NXT) is one of the most prevalent Traditional Chinese Medicine formulations in the treatment of coronary heart disease (CHD), yet the action of pharmacodynamic components remains unclear.

Objective: To determine the basis by which pharmacodynamic components of NXT may be effective in the treatment of CHD.

Materials And Methods: The protective effect of NXT (0.01-100 μg/mL) on 293 T and hy926 cells was determined by MTT assay for 24 h. Afterwards, to investigate the pharmacodynamic material basis of NXT in anti-inflammatory and antioxidant effects, based on previous UPLC/Q-TOF analysis, 293 T and hy926 cells were divided into control (treated with solvent), model (incubated with TNF-α, LPS or HO), intervention (treated with UPLC components) and positive groups. After 24 h of treatment, all cells were tested to verify the screening results. MOE software was applied to dock bioactive compounds with phosphoinositide 3-kinase (PI3K), then the protein expression and phosphate levels were determined by western blotting.

Results: NXT could significantly inhibit the expression of NF-κB, MMP-9 and NO in cells with IC values of 0.1178, 0.1182 and 0.1094 μg/mL. Based on the screening results, six components of NXT were identified (calycosin, ferulic acid, salvianolic acid B, ononin, salvianolic acid E, and salvianolic acid F) which can inhibit NF-κB, MMP-9, and NO simultaneously, while exerting cytoprotective effects by inhibiting the activation of the PI3K/AKT pathway under different conditions by virtue of their advantageous interaction with PI3K.

Conclusions: These ingredients have outstanding therapeutic potential and may provide a scientific basis for the future application and research of NXT.
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http://dx.doi.org/10.1080/13880209.2020.1870506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079059PMC
December 2021

Proliferative glomerulonephritis with monoclonal immunoglobulin G deposits in a young woman: A case report.

World J Clin Cases 2021 Apr;9(10):2357-2366

Department of Nephrology, Shenzhen Longhua District Central Hospital, Shenzhen 518110, Guangdong Province, China.

Background: Proliferative glomerulonephritis with monoclonal immunoglobulin G (IgG) deposits (PGNMID) is a newly recognized rare disease. The renal pathology is characterized by prominent manifestations of membranous hyperplasia, which are easy to misdiagnose. The clinical symptoms are severe. Massive proteinuria and hypoproteinemia are conspicuous, and most patients are accompanied by renal insufficiency and microscopic hematuria.

Case Summary: A 27-year-old woman was admitted to a hospital for macroscopic hematuria and proteinuria 4 years prior, and renal biopsy in the hospital suggested moderate-to-severe mesangial proliferating glomerulonephritis (MsPGN). She had taken a glucocorticoid, cyclophosphamide, mycophenolate mofetil, and other treatments and achieved brief partial remission. Recently, the patient visited our hospital due to massive proteinuria. Repeated renal biopsy and re-evaluation of the first biopsy obtained 4 years previously revealed monoclonal immunoglobulin deposition in the glomeruli. A bone marrow examination was performed to exclude hematologic malignancy, and a diagnosis of PGNMID was established. The patient showed remission after four cycles of a bortezomib + cyclophosphamide + dexamethasone scheme.

Conclusion: PGNMID is usually misdiagnosed as MsPGN or membranoproliferative glomerulonephritis. Although it often occurs in middle-aged and elderly individuals, it cannot be readily excluded in young people, even when serum immunofixation electrophoresis is negative. IgG subtype and light chain staining are necessary when this disease is highly suspected. An accurate diagnosis at the earliest stage may avoid the overuse of glucocorticoids and immunosuppressants.
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http://dx.doi.org/10.12998/wjcc.v9.i10.2357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026847PMC
April 2021

Generation of a human induced pluripotent stem cell line from a Bietti crystalline corneoretinal dystrophy patient with CYP4V2 mutations.

Stem Cell Res 2021 05 7;53:102330. Epub 2021 Apr 7.

Department of Ophthalmology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Bietti crystalline corneoretinal dystrophy (BCD) is an autosomal recessively inherited progressive retinal disease. Here, we describe the generation and characterization of a human induced pluripotent stem cell (hiPSC) line of BCD patient with CYP4V2 mutations. The reprogramming of this iPSC line was performed from skin fibroblast by using the Sendai-virus based approach. The established hiPSC line retained the disease-associated mutations and showed normal karyotype, pluripotency and differentiation capacity.
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http://dx.doi.org/10.1016/j.scr.2021.102330DOI Listing
May 2021

Afterload-related cardiac performance identifies cardiac impairment and associates with outcome in patients with septic shock: a retrospective cohort study.

J Intensive Care 2021 Apr 13;9(1):33. Epub 2021 Apr 13.

Department of Cardiology, Heart Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Background: Septic patients with cardiac impairment are with high mortality. Afterload-related cardiac performance (ACP), as a new tool for diagnosing septic cardiomyopathy (SCM), still needs to be evaluated for its impact on the prognosis for patients with septic shock.

Methods: In this retrospective study, 100 patients with septic shock undertaken PiCCO monitoring were included. The ability of ACP, cardiac index (CI), and cardiac power index (CPI) to discriminate between survivors and non-survivors was tested by comparing the area under the receiver operating characteristic curve (AUROC) analysis. Cox proportional hazards regression analyses were performed to assess the associations of ACP with day-28 mortality. Curve estimation was used to describe the relationship between the hazard ratio (HR) of death and ACP.

Results: ACP had a strong linear correlation with CI and CPI (P < 0.001). ACP demonstrated significantly greater discrimination for day-28 mortality than CI before adjusted [AUROC 0.723 (95% CI 0.625 to 0.822) vs. 0.580 (95% CI 0.468 to 0.692), P = 0.007] and CPI after adjusted [AUROC 0.693 (95% CI 0.590 to 0.797) vs. 0.448 (0.332 to 0.565), P < 0.001]. Compared with ACP > 68.78%, HR for ACP ≤ 68.78% was 3.55 (1.93 to 6.54) (P < 0.001). When adjusted with age, APACHE-II score, Vasoactive Inotropic Score, Lactate, CRRT, day-1 volume, fibrinogen and total bilirubin as possible confounders, and decrease ACP are still associated with increasing day-28 mortality (P < 0.05). An exponential relationship was observed between ACP12h and HR of day-28 death.

Conclusions: Our results suggested thatACP could improve mortality predictions when compared to CI and CPI. Decreased ACP was still an independent risk factor for increased day-28 mortality.
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http://dx.doi.org/10.1186/s40560-021-00549-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042871PMC
April 2021

Whole Genome Sequencing in the Evaluation of Fetal Structural Anomalies: A Parallel Test with Chromosomal Microarray Plus Whole Exome Sequencing.

Genes (Basel) 2021 03 6;12(3). Epub 2021 Mar 6.

Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 201204, China.

Whole genome sequencing (WGS) is a powerful tool for postnatal genetic diagnosis, but relevant clinical studies in the field of prenatal diagnosis are limited. The present study aimed to prospectively evaluate the utility of WGS compared with chromosomal microarray (CMA) and whole exome sequencing (WES) in the prenatal diagnosis of fetal structural anomalies. We performed trio WGS (≈40-fold) in parallel with CMA in 111 fetuses with structural or growth anomalies, and sequentially performed WES when CMA was negative (CMA plus WES). In comparison, WGS not only detected all pathogenic genetic variants in 22 diagnosed cases identified by CMA plus WES, yielding a diagnostic rate of 19.8% (22/110), but also provided additional and clinically significant information, including a case of balanced translocations and a case of intrauterine infection, which might not be detectable by CMA or WES. WGS also required less DNA (100 ng) as input and could provide a rapid turnaround time (TAT, 18 ± 6 days) compared with that (31 ± 8 days) of the CMA plus WES. Our results showed that WGS provided more comprehensive and precise genetic information with a rapid TAT and less DNA required than CMA plus WES, which enables it as an alternative prenatal diagnosis test for fetal structural anomalies.
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http://dx.doi.org/10.3390/genes12030376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999180PMC
March 2021

Adherence to Multidrug Resistant Tuberculosis Treatment and Case Management in Chongqing, China - A Mixed Method Research Study.

Infect Drug Resist 2021 15;14:999-1012. Epub 2021 Mar 15.

Department of Social Medicine and Health Service Management, Army Medical University (Third Military Medical University), Chongqing, People's Republic of China.

Aim: This paper evaluated the treatment adherence for multidrug-resistant tuberculosis (MDR-TB) and MDR-TB case management (MTCM) in Chongqing, China in order to identify factors associated with poor treatment adherence and case management.

Methods: Surveys with 132 MDR-TB patients and six in-depth interviews with health care workers (HCWs) from primary health centers (PHC), doctors from MDR-TB designated hospitals and MDR-TB patients were conducted. Surveys collected demographic and socio-economic characteristics, as well as factors associated with treatment and case management. In-depth interviews gathered information on treatment and case management experience and adherence behaviors.

Results: Patient surveys found the two main reasons for poor adherence were negative side-effects from the treatment and busy work schedules. In-depth interviews with key stakeholders found that self-perceived symptom improvement, negative side-effects from treatment and financial difficulties were the main reasons for poor adherence. MDR-TB patients from urban areas, who were unmarried, were female, had migrant status, and whose treatments were supervised by health care workers from primary health clinics, had poorer treatment adherence (P<0.05). Among the MDR-TB patients surveyed, 86.7% received any type of MTCM in general (received any kind of MTCM from HCWs in PHC, MDR-TB designated hospital and centers of disease control/TB dispensaries and 62.50% received MTCM from HCWs in PHC sectors). Patients from suburban areas were more likely to receive both MTCM in general (OR=6.70) and MTCM from HCWs in MDR-TB designated hospitals (OR=2.77), but female patients (OR=0.26) were less likely to receive MTCM from HCWs in PHC sectors, and patients who were not educated about MTCM by TB doctors in designated hospitals were less likely to receive MTCM in general (OR=0.14). Patients who had not been hospitalized were less likely to receive MTCM from HCWs in MDR-TB designated hospitals (OR=0.21).

Conclusion: Stronger MTCM by HCWs in PHC sectors would improve treatment adherence among MDR-TB patients. Community-based patient-centered models of MTCM in PHC sectors and the use of digital health technology could help to improve case management and thereby improve adherence.
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http://dx.doi.org/10.2147/IDR.S293583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979342PMC
March 2021

A 14-gene gemcitabine resistance gene signature is significantly associated with the prognosis of pancreatic cancer patients.

Sci Rep 2021 Mar 17;11(1):6087. Epub 2021 Mar 17.

Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.

To identify a gemcitabine resistance-associated gene signature for risk stratification and prognosis prediction in pancreatic cancer. Pearson correlation analysis was performed with gemcitabine half maximal inhibitory concentration (IC50) data of 17 primary pancreatic cancer lines from Genomics of Drug Sensitivity in Cancer (GDSC) and the transcriptomic data from GDSC and Broad Institute Cancer Cell Line Encyclopedia, followed by risk stratification, expression evaluation, overall survival (OS) prediction, clinical data validation and nomogram establishment. Our biomarker discovery effort identified a 14-gene signature, most of which featured differential expression. The 14-gene signature was associated with poor OS in E-MTAB-6134 (HR 2.37; 95% CI 1.75-3.2; p < 0.0001), pancreatic cancer-Canada (PACA-CA) (HR 1.76; 95% CI 1.31-2.37; p = 0.00015), and 4 other independent validation cohorts: pancreatic cancer-Australia (PACA-AU) (HR 1.9; 95% CI 1.38-2.61; p < 0.0001), The Cancer Genome Atlas (TCGA) (HR 1.73; 95% CI 1.11-2.69; p = 0.014), GSE85916 (HR 1.97; 95% CI 1.14-3.42; p = 0.014) and GSE62452 (HR 1.82; 95% CI 1.02-3.24; p = 0.039). Multivariate analysis revealed that the 14-gene risk score was an independent pancreatic cancer outcome predictor in E-MTAB-6134 (p < 0.001) and TCGA (p = 0.006). A nomogram including the 14-gene was established for eventual clinical translation. We identified a novel gemcitabine resistance gene signature for risk stratification and robust categorization of pancreatic cancer patients with poor prognosis.
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http://dx.doi.org/10.1038/s41598-021-85680-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969955PMC
March 2021

Pituitary tumor apoplexy associated with extrapontine myelinolysis during pregnancy: A case report.

Medicine (Baltimore) 2021 Mar;100(10):e25075

Department of Radiology.

Rationale: Pituitary tumor apoplexy (PTA) is a rare clinical syndrome which requires urgent diagnosis and treatment due to its life-threatening consequences. Management of undiagnosed pituitary tumor before pregnancy is a problem during pregnancy.

Patient Concerns: We reported a case with PTA which was not diagnosed before pregnancy presenting with vomiting associated with hyponatremia during the third trimester. After supplying the sodium the patient presented with dysarthria and hemiplegia.

Diagnoses: MRI examination showed PTA accompanied with extrapontine myelinolysis (EPM).

Interventions: The patient was given hydrocortisone according to the symptoms gradually to taper off dose, at the same times oral levothyroxine therapy (25μg/day) was given.

Outcomes: The patient delivered a healthy baby via cesarean section at hospital at 38 + 1 week of gestation. We performed MRI examination regularly and the tumor regressed significantly 8 months postpartum.

Lessons: We reported a case as PTA associated with EPM. Headache during pregnancy is often nonspecific, so careful medical history inquiry is very important.
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http://dx.doi.org/10.1097/MD.0000000000025075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969261PMC
March 2021

Can R ' mapping evaluate hypoxia in renal ischemia reperfusion injury quantitatively? An experimental study.

Magn Reson Med 2021 08 16;86(2):974-983. Epub 2021 Mar 16.

Department of Radiology, Third Affiliated Hospital of Soochow University, Changzhou, People's Republic of China.

Purpose: To explore if R ' mapping can assess renal hypoxia in rabbits with ischemia reperfusion injury (IRI).

Methods: Forty rabbits were randomly divided into 4 groups according to the clipping time: the sham group and 45 min, 60 min, and 75 min for the mild, moderate, and severe groups (with n = 10 each group), respectively. Intravenous furosemide (FU) was administered 24 h after IRI. All rabbits were performed 5 times (IRI , IRI , FU , FU , and FU ) with a 3.0 Tesla MR. The R ' values and the hypoxic scores were then recorded. The repeated measurement analysis of variance and Spearman correlation analysis was used for statistical analysis.

Results: Compared to the baseline, the medullary R ' values increased significantly 24 h after the IRI (baseline 19.31 ± 1.21 s , mild group 20.05 ± 1.26 s , moderate group 25.38 ± 1.38 s , and severe group 25.79 ± 1.10 s ; each P < .001). FU led to a significant decrease in the medullary R ' value (sham group 11.17 ± 4.33 s , mild group 7.80 ± 0.74 s , moderate group 3.92 ± 0.28 s , and severe group 3.82 ± 0.23 s ; each P < .05). Quantitative hypoxic scores revealed significant differences among the 4 groups in the outer medulla (P < .001 each). The medullary R ' differences (before and after intravenous FU) were significantly correlated with the hypoxic scores, respectively (P < .001).

Conclusion: R ' mapping can evaluate the renal hypoxia in the procession of IRI in rabbits and might serve as a quantitative biomarker for IRI.
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http://dx.doi.org/10.1002/mrm.28696DOI Listing
August 2021

Radiomics: The Next Frontier of Cardiac Computed Tomography.

Circ Cardiovasc Imaging 2021 03 16;14(3):e011747. Epub 2021 Mar 16.

Department of Diagnostic Radiology, Jinling Hospital, the First School of Clinical Medicine, Southern Medical University, Nanjing, Jiangsu Province, China (Y.X., Y.Y., L.Z.).

Radiomics uses advanced image analysis to extract massive amounts of quantitative information from digital images, which is not otherwise distinguishable to the human eye. The mined data can be used to explore and establish new and undiscovered correlations between these imaging features and clinical end points. Cardiac computed tomography (CT) is a first-line imaging modality for evaluating coronary artery disease and has a primary role in the assessment of cardiac structures. Conventional interpretation of cardiac CT images relies mostly on subjective and qualitative analysis, as well as basic geometric quantification. To date, some proof-of-concept studies have demonstrated the feasibility and diagnostic performance of cardiac CT radiomics analysis. This review describes the current literature on radiomics in cardiac CT and discusses its advantages, challenges, and future directions. Although much evidences are needed in this field, cardiac CT radiomics has a lot to offer to patients and physicians with potential to define cardiac disease phenotypes on imaging with higher precision.
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http://dx.doi.org/10.1161/CIRCIMAGING.120.011747DOI Listing
March 2021
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