Publications by authors named "Xing Ma"

155 Publications

Serum macrophage migration inhibitory factor as a potential biomarker to evaluate therapeutic response in patients with allergic asthma: an exploratory study.

J Zhejiang Univ Sci B 2021 Jun;22(6):512-520

Department of Pulmonary and Critical Care Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

: Previous studies have shown that macrophage migration inhibitory factor (MIF) is involved in the pathogenesis of asthma. This study aimed to investigate whether serum MIF reflects a therapeutic response in allergic asthma. : We enrolled 30 asthmatic patients with mild-to-moderate exacerbations and 20 healthy controls, analyzing the parameter levels of serum MIF, serum total immunoglobulin E (tIgE), peripheral blood eosinophil percentage (EOS%), and fractional exhaled nitric oxide (FeNO). Lung function indices were used to identify disease severity and therapeutic response. : Our study showed that all measured parameters in patients were at higher levels than those of controls. After one week of treatment, most parameter levels decreased significantly except for serum tIgE. Furthermore, we found that serum MIF positively correlated with EOS% as well as FeNO, but negatively correlated with lung function indices. Receiver operator characteristic (ROC) curve analysis indicated that among the parameters, serum MIF exhibited a higher capacity to evaluate therapeutic response. The area under the curve (AUC) of MIF was 0.931, with a sensitivity of 0.967 and a specificity of 0.800. : Our results suggested that serum MIF may serve as a potential biomarker for evaluating therapeutic response in allergic asthma with mild-to-moderate exacerbations.
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http://dx.doi.org/10.1631/jzus.B2000555DOI Listing
June 2021

Aptamer-based lateral flow assay on-site biosensors.

Biosens Bioelectron 2021 Apr 24;186:113279. Epub 2021 Apr 24.

School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, 611731, China. Electronic address:

The lateral flow assay (LFA) is a widely used paper-based on-site biosensor that can detect target analytes and obtain test results in several minutes. Generally, antibodies are utilized as the biorecognition molecules in the LFA. However, antibodies selected using an in vivo process not only may risk killing the animal hosts and causing errors between different batches but also their range is restricted by the refrigerated conditions used to store them. To avoid these limitations, aptamers screened by an in vitro process have been studied as biorecognition molecules in LFAs. Based on the sandwich or competitive format, the aptamer-based LFA can accomplish on-site detection of target analytes. Since aptamers have a distinctive ability to undergo conformational changes, the adsorption-desorption format has also been exploited to detect target analytes in aptamer-based LFAs. This paper reviews developments in aptamer-based LFAs in the last three years for the detection of target analytes. Three formats of aptamer-based LFAs, i.e., sandwich, competitive, and adsorption-desorption, are described in detail. Based on these formats, signal amplification strategies and multiplexed detection are discussed in order to provide an overview of aptamer-based LFAs for on-site detection of target analytes. In addition, the potential commercialization and future perspectives of aptamer-based LFAs for rapid detection of SARS-CoV-2 are given to support the COVID-19 pandemic.
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http://dx.doi.org/10.1016/j.bios.2021.113279DOI Listing
April 2021

Estimation of trapezoidal-shaped overlapping nuclear pulse parameters based on a deep learning CNN-LSTM model.

J Synchrotron Radiat 2021 May 19;28(Pt 3):910-918. Epub 2021 Apr 19.

College of Nuclear Technology and Automation Engineering, Chengdu University of Technology, Dongsanlu, Erxianqiao, Chengdu 610059, People's Republic of China.

The Long Short-Term Memory neural network (LSTM) has excellent learning ability for the time series of the nuclear pulse signal. It can accurately estimate the parameters (such as amplitude, time constant, etc.) of the digitally shaped nuclear pulse signal (especially the overlapping pulse signal). However, due to the large number of pulse sequences, the direct use of these sequences as samples to train the LSTM increases the complexity of the network, resulting in a lower training efficiency of the model. The convolution neural network (CNN) can effectively extract the sequence samples by using its unique convolution kernel structure, thus greatly reducing the number of sequence samples. Therefore, the CNN-LSTM deep neural network is used to estimate the parameters of overlapping pulse signals after digital trapezoidal shaping of exponential signals. Firstly, the estimation of the trapezoidal overlapping nuclear pulse is considered to be obtained after the superposition of multiple exponential nuclear pulses followed by trapezoidal shaping. Then, a data set containing multiple samples is set up; each sample is composed of the sequence of sampling values of the trapezoidal overlapping nuclear pulse and the set of shaping parameters of the exponential pulse before digital shaping. Secondly, the CNN is used to extract the abstract features of the training set in these samples, and then these abstract features are applied to the training of the LSTM model. In the training process, the pulse parameter set estimated by the present neural network is calculated by forward propagation. Thirdly, the loss function is used to calculate the loss value between the estimated pulse parameter set and the actual pulse parameter set. Finally, a gradient-based optimization algorithm is applied to update the weight by getting back the loss value together with the gradient of the loss function to the network, so as to realize the purpose of training the network. After model training was completed, the sampled values of the trapezoidal overlapping nuclear pulse were used as input to the CNN-LSTM model to obtain the required parameter set from the output of the CNN-LSTM model. The experimental results show that this method can effectively overcome the shortcomings of local convergence of traditional methods and greatly save the time of model training. At the same time, it can accurately estimate multiple trapezoidal overlapping pulses due to the wide width of the flat top, thus realizing the optimal estimation of nuclear pulse parameters in a global sense, which is a good pulse parameter estimation method.
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http://dx.doi.org/10.1107/S1600577521003441DOI Listing
May 2021

Chinese adolescents' sexual and reproductive health education: A quasi-experimental study.

Public Health Nurs 2021 May 5. Epub 2021 May 5.

The Nethersole School of Nursing, Faculty of Medicine, The Chinese University of Hong Kong, HKSAR.

Objective: This study investigated the effectiveness of an interactive sexual and reproductive health education program in aspects of knowledge, attitudes, and self-efficacy among adolescents.

Design: Quasi-experimental study underpinned by social cognitive theory.

Sample: A stratified cluster sample of 469 students from the two-branch middle school in a city in eastern China who were assigned to the experimental (n = 233) and control (n = 236) groups.

Measurements: Students' sexual knowledge, attitudes, and refusal self-efficacy were assessed before (T0), immediately after (T1), and 1 month after the intervention (T2), respectively.

Intervention: Students in the experimental group received two 40-min sessions of the educational program while the control group received the usual mode of sexual and reproductive health education.

Results: Compared with the control group, students in the experimental group acquired more sexual knowledge (p < .01), and developed more positive sexual attitudes (p < .05) and stronger sexual self-efficacy (p < .05) across the study period.

Conclusions: The proposed sexual and reproductive health education program incorporating various interactive activities was effective and could be used for school-based implementation led by nurses and other health care workers.
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http://dx.doi.org/10.1111/phn.12914DOI Listing
May 2021

A sensitive and quantitative prognosis of C-reactive protein at picogram level using mesoporous silica encapsulated core-shell up-conversion nanoparticle based lateral flow strip assay.

Talanta 2021 Aug 23;230:122335. Epub 2021 Mar 23.

School of Automation Engineering, University of Electronic Science and Technology of China, Chengdu, 611731, PR China. Electronic address:

C- reactive protein (CRP) is a sensitive indicator for infectious or inflammatory diseases in human which can reflect the body's inflammation latency and early pathophysiological changes. The most common detection method of serum CRP is ELISA that has been proved to be expensive and time-consuming, restricting its use in point-of-care application. In this paper, we demonstrated a lateral flow system for CRP quantification by using mesoporous silica (mSiO) coated up-converting nanoparticles (UCNPs) (denoted as [email protected]) as fluorescent labels. The up-converting core can emit strong green fluorescence signals under NIR excitation light (980 nm) with excellent photostability, high signal-to-noise ratio and low background fluorescence. By wrapping ultrathin mSiO outside, the core-shell structured [email protected] exhibits good dispersity and stability meanwhile maintains strong fluorescence emission. Besides, the mSiO shell provides further functionalities for antibody linkage. By using a portable fluorescence sensor, we reached a CRP detection limit of 0.05 ng/mL and a linear range from 0.1 ng/mL-50 ng/mL, and the detection time was no more than 8 min. The lateral flow test strips exhibit great stability in CRP quantification (CV%<5) and have a life time of more than 1 week at ambient temperature. Furthermore, the proposed system can work with a cloud-enabled smartphone through Bluetooth for Internet of Medical Things application. This CRP detection method proves to be rapid and easy-operated, which has great potential in early inflammatory disease perception in the point-of-care tests and future's 5G-enabled remote healthcare management.
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http://dx.doi.org/10.1016/j.talanta.2021.122335DOI Listing
August 2021

Microscopic Afterglow Bioimaging by Ultralong Organic Phosphorescent Nanoparticles in Living Cells and Zebrafish.

Anal Chem 2021 04 14;93(16):6516-6522. Epub 2021 Apr 14.

Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, China.

Compared with short-lived emission probes featuring fluorescence imaging , the use of phosphorescent probes imparts the advantage of long-lived signal persistence that distinguishes against background fluorescence interference. However, the realization of ultralong organic phosphorescent (UOP) probes with an ultralong emission lifetime in an aqueous medium is still a challenge. Here, we present a rational strategy for obtaining UOP nanoparticles (NPs) in an air-saturated aqueous medium prepared using an organic phosphor (PDBCz) and a surfactant polymer (PVP), named [email protected], showing an ultralong emission lifetime of 284.59 ms and a phosphorescence quantum efficiency of 7.6%. The excellent phosphorescence properties and water solubility of [email protected] make it a promising candidate for biological imaging. The as-prepared [email protected] NPs possess excellent luminescence intensity as well as illustrious biocompatibility both in vitro and in vivo. We demonstrate their use as an efficient phosphorescent nanoprobe both in living cells and zebrafish by capturing their afterglow emission signals under microscopy observation for the first time, realizing convenient and fast bioimaging with low cost, which allows for anti-fluorescence interference and shows promise for the future theragnostic applications in nanomedicine.
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http://dx.doi.org/10.1021/acs.analchem.1c00423DOI Listing
April 2021

5G-enabled ultra-sensitive fluorescence sensor for proactive prognosis of COVID-19.

Biosens Bioelectron 2021 Jun 13;181:113160. Epub 2021 Mar 13.

School of Automation Engineering, University of Electronic Science and Technology of China, Chengdu, 611731, PR China. Electronic address:

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is spreading around the globe since December 2019. There is an urgent need to develop sensitive and online methods for on-site diagnosing and monitoring of suspected COVID-19 patients. With the huge development of Internet of Things (IoT), the impact of Internet of Medical Things (IoMT) provides an impressive solution to this problem. In this paper, we proposed a 5G-enabled fluorescence sensor for quantitative detection of spike protein and nucleocapsid protein of SARS-CoV-2 by using mesoporous silica encapsulated up-conversion nanoparticles ([email protected]) labeled lateral flow immunoassay (LFIA). The sensor can detect spike protein (SP) with a detection of limit (LOD) 1.6 ng/mL and nucleocapsid protein (NP) with an LOD of 2.2 ng/mL. The feasibility of the sensor in clinical use was further demonstrated by utilizing virus culture as real clinical samples. Moreover, the proposed fluorescence sensor is IoMT enabled, which is accessible to edge hardware devices (personal computers, 5G smartphones, IPTV, etc.) through Bluetooth. Medical data can be transmitted to the fog layer of the network and 5G cloud server with ultra-low latency and high reliably for edge computing and big data analysis. Furthermore, a COVID-19 monitoring module working with the proposed the system is developed on a smartphone application (App), which endows patients and their families to record their medical data and daily conditions remotely, releasing the burdens of going to central hospitals. We believe that the proposed system will be highly practical in the future treatment and prevention of COVID-19 and other mass infectious diseases.
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http://dx.doi.org/10.1016/j.bios.2021.113160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954646PMC
June 2021

Anti-SARS-CoV-2 IgG and IgM detection with a GMR based LFIA system.

Talanta 2021 May 10;227:122207. Epub 2021 Feb 10.

School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, 611731, PR China. Electronic address:

Since December 2019, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused millions of deaths and seriously threatened the safety of human life; indeed, this situation is worsening and many people are infected with the new coronavirus every day. Therefore, it is very important to understand patients' degree of infection and infection history through antibody testing. Such information is useful also for the government and hospitals to formulate reasonable prevention policies and treatment plans. In this paper, we develop a lateral flow immunoassay (LFIA) method based on superparamagnetic nanoparticles (SMNPs) and a giant magnetoresistance (GMR) sensing system for the simultaneously quantitative detection of anti-SARS-CoV-2 immunoglobulin M (IgM) and G (IgG). A simple and time-effective co-precipitation method was utilized to prepare the SMNPs, which have good dispersibility and magnetic property, with an average diameter of 68 nm. The Internet of Medical Things-supported GMR could transmit medical data to a smartphone through the Bluetooth protocol, making patient information available for medical staff. The proposed GMR system, based on SMNP-supported LFIA, has an outstanding advantage in cost-effectiveness and time-efficiency, and is easy to operate. We believe that the suggested GMR based LFIA system will be very useful for medical staff to analyze and to preserve as a record of infection in COVID-19 patients.
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http://dx.doi.org/10.1016/j.talanta.2021.122207DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874965PMC
May 2021

Magnetic Resistance Sensory System for the Quantitative Measurement of Morphine.

IEEE Trans Biomed Circuits Syst 2021 Feb 30;15(1):171-176. Epub 2021 Mar 30.

Point-of-care testing (POCT) is characterized by fast detection, simple use, and cost efficiency. As the focus of healthcare shifts to precision medicine, population health, and chronic disease management, the potential impact of POCT has increased significantly in the past decade. Immunochromatographic test strips (ICTSs) are currently the most promising POCT diagnostic format due to the advantages of fast detection, simple operation and cost-effective. The lateral flow immune analyzer (LFIA) system that uses magnetic resistance (MR) sensors as readers and magnetic nanoparticle labeling materials has attracted wide attention due to its high sensitivity and specificity. This paper designs a quantitative lateral flow immunoassay instrument for MR sensors using InSb single crystals as magnetoresistive elements. MR sensors detect samples of ICTSs labeled with superparamagnetic nanoparticles (SMNPs). The collected weak magnetic signal is processed by the designed amplifying circuit and software algorithm. As a result, the MR system can quantitatively detect the concentration of morphine (MOP), and has good repeatability. Compared with other optical detection instruments, the system has good specificity because there is very low background noise in the detection sample. The size of the whole system is [Formula: see text], and it has the characteristics of compactness and fast detection.
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http://dx.doi.org/10.1109/TBCAS.2021.3060181DOI Listing
February 2021

GINS2 affects cell proliferation, apoptosis, migration and invasion in thyroid cancer via regulating MAPK signaling pathway.

Mol Med Rep 2021 04 4;23(4). Epub 2021 Feb 4.

Department of General Surgery, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.

Globally, thyroid cancer (TC) is considered to be the commonest endocrine malignancy. GINS complex subunit 2 (GINS2) belongs to the GINS complex family and is associated with cellular migration, invasion and growth. The present study aimed to investigate the underlying mechanisms of GINS2 on cell viability, migration and invasion in TC cells. By using MTT, wound healing and Transwell assays, the cell viability, migration and invasion were determined. Apoptosis was examined by immunofluorescence. Western blotting was used to detect protein expression levels. In the present study, biological function analysis demonstrated that GINS2 interference attenuated cell viability, migration and invasion in TC cell lines (K1 and SW579). It was discovered that, compared with the control group, GINS2 silencing induced apoptosis in TC cells. Additionally, GINS2 interference inhibited key proteins in the MAPK signaling pathway, including JNK, ERK and p38. According to these comparative experiments, GINS2 was considered to act a pivotal part in cell viability, migration and invasion of TC by regulating the MAPK signaling pathway and might be a potential therapeutic target for treating TC.
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http://dx.doi.org/10.3892/mmr.2021.11885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893785PMC
April 2021

Magnetically steerable iron oxides-manganese dioxide core-shell micromotors for organic and microplastic removals.

J Colloid Interface Sci 2021 Apr 29;588:510-521. Epub 2020 Dec 29.

State Key Laboratory of Advanced Welding and Joining, Flexible Printed Electronic Technology Center, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China; Shenzhen Bay Laboratory, No. 9 Duxue Road, Shenzhen 518050, China. Electronic address:

Because of micro/nanoscale manipulation and task-performing capability, micro/nanomotors (MNMs) have attracted lots of research interests for potential applications in biomedical and environmental applications. Owing to the low-cost, good motion behavior, and environmental friendliness, various low-cost metal oxides based MNMs become promising alternatives to the precious metal based MNMs, in particular for environmental remediation applications. Hereby, we demonstrate the facile and scalable fabrication of two types of bubble-propelled iron oxides-MnO core-shell micromotors (FeO-MnO and FeO-MnO) for pollutant removal. The FeO-MnO micromotor exhibits efficient removals of both aqueous organics and suspended microplastics via the synergy of catalytic degradation, surface adsorption, and adsorptive bubbles separations mechanisms. The adsorptive bubbles separation achieved more than 10% removal of the suspended microplastics from the polluted water in 2 h. We clarified the major contributions of different remediation mechanisms in pollutants removals, and the findings may be beneficial to a wide range of environmental applications of MNMs.
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http://dx.doi.org/10.1016/j.jcis.2020.12.097DOI Listing
April 2021

Undercorrection: the undesired effect of compression on the osteotomy gap of the medial opening wedge high tibial osteotomy and its clinical significance.

Arch Orthop Trauma Surg 2021 Jan 8. Epub 2021 Jan 8.

Department of Orthopedic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.

Introduction: Undercorrection is a common problem in opening wedge high tibial osteotomy (OWHTO). We investigated the compression effect of cortex screw on the osteotomy gap and its clinical significance.

Materials And Methods: A standard OWHTO using the TomoFix plate was conducted on 20 bone models in two groups to get a 10-mm medial osteotomy gap. A cortex screw was used temporarily in a neutral (at the center) and an eccentric position (near the inclined plane) of the dynamic hole in group 1 and group 2, respectively. The mean of undercorrection observed in the two groups was compared using an independent t test. Also, the effect of compression on the gap between the plate and medial tibial cortex, and the osteotomy gap was evaluated using a Sine rule. Besides, the mean undercorrection observed was assessed for clinical significance based on the effect on the weight-bearing axis (WBA) using a Cosine Rule.

Results: The mean undercorrection was 1.3 ± 0.6 mm and 2.6 ± 0.6 mm in group 1 and group 2, respectively. A significantly greater undercorrection was observed in group 2 (p < 0.001). The correction loss in group 2 has resulted from combinations of the sliding effect of the dynamic hole and oblique compression effect over the gap between the plate and medial tibial cortex whereas in group 1 it has only resulted from the oblique compression effect. The observed undercorrection in group 2 has resulted in clinically significant WBA shift (10%) over the width of the tibial plateau.

Conclusions: In OWHTO, compression is important for the stability and healing of osteotomy, but it can also cause loss of correction. In patients requiring large correction, the surgeon should control the amount of compression required and consider making extra osteotomy gap to avoid undercorrection. Furthermore, the placement of cortex screws in neutral is essential to lower the risk of undercorrection.
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http://dx.doi.org/10.1007/s00402-020-03717-8DOI Listing
January 2021

Identify Molecular Mechanisms of Jiangzhi Decoction on Nonalcoholic Fatty Liver Disease by Network Pharmacology Analysis and Experimental Validation.

Biomed Res Int 2020 12;2020:8829346. Epub 2020 Dec 12.

The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, 358 Datong Road, Pudong, Shanghai 200137, China.

Background: Jiangzhi Decoction (JZD), a traditional herb mixture, has shown significant clinical efficacy against nonalcoholic fatty liver disease (NAFLD). However, its multicomponent and multitarget characteristics bring difficulty in deciphering its pharmacological mechanisms. Our study is aimed at identifying the core molecular mechanisms of JZD against NAFLD.

Methods: The active ingredients were searched from Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and Traditional Chinese Medicine Integrated Database (TCMID). The targets of those ingredients were identified using ChemMapper database based on 3D structure similarity. NAFLD-related genes were searched from DisGeNET database and Gene Expression Omnibus (GEO) database. Then, we performed protein-protein interaction (PPI) analysis, functional enrichment analysis, and constructed pathway networks of "herbs-active ingredients-candidate targets" and identified the core molecular mechanisms and key active ingredients in the network. Also, molecular docking was carried out to predict the ligands of candidate targets using SwissDock. Finally, the human hepatic L02 cell line was used to establish the NAFLD model The effect and key molecules were validated by Oil Red O staining, biochemical assays, and quantitative real-time PCR (qRT-PCR).

Results: We found 147 active ingredients in JZD, 1285 targets of active ingredients, 401 NAFLD-related genes, and 59 overlapped candidate targets of JZD against NAFLD. 22 core targets were obtained by PPI analysis. Finally, nuclear receptor transcription and lipid metabolism regulation were found as the core molecular mechanisms of JZD against NAFLD by functional enrichment analysis. The candidate targets PPAR and LXR were both docked with hyperin as the most favorable interaction, and HNF4 was docked with linolenic acid ethyl ester. According to experiments, it was found that JZD had an inhibitory effect on lipid accumulation and regulatory effects on cholesterol and triglycerides. Compared with OA group, the mRNA expression levels of and were significantly upregulated in JZD group ( < 0.05), and was significantly downregulated ( < 0.001).

Conclusion: JZD might alleviate hepatocyte steatosis by regulating some key molecules related to nuclear receptor transcription and lipid metabolism, such as , , and . Our study will provide the scientific evidences of the clinical efficacy of JZD against NAFLD.
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http://dx.doi.org/10.1155/2020/8829346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753939PMC
December 2020

Identification of Pectobacterium versatile causing blackleg of potato in New York State.

Plant Dis 2021 Jan 6. Epub 2021 Jan 6.

USDA-ARS, Plant-microbe Interactions, 322 Plant Science, Cornell University, Ithaca, New York, United States, 14853.

Soft rot bacteria classified in the Pectobacteriaceae (SRP), including Pectobacterium and Dickeya spp., are responsible for soft rot and blackleg diseases of potato. Since 2014, blackleg outbreaks caused by D. dianthicola have increased in the US and Canada. Our previous study found that the most abundant causal organisms of blackleg disease in New York State were P. parmentieri and D. dianthicola, with the latter being the only Dickeya species reported. In the present study, we identified and characterized pathogenic SRP bacteria from 19 potato samples collected in New York State during the 2017 growing season. We used genome sequence comparison to determine the pathogens' species. We found eight P. versatile, one P. atrosepticum, two P. carotovorum, two P. parmentieri, and six D. dianthicola isolates in our 2017 sample SRP collection. This is the first time that P. versatile is reported to cause potato blackleg disease in New York State. We determined the phylogenetic relationships between the SRP strains using 151 single copy orthologous gene sequences shared among the set of bacteria in our analysis, which provided better resolution than phylogenies constructed using the dnaX gene.
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http://dx.doi.org/10.1094/PDIS-09-20-2089-REDOI Listing
January 2021

Mesoporous Nanostructures Encapsulated with Metallic Nanodots for Smart SERS Sensing.

ACS Appl Mater Interfaces 2021 Jan 22;13(1):186-195. Epub 2020 Dec 22.

Flexible Printed Electronic Technology Center and School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China.

In virtue of uniform mesopores and core-shell nanoarchitectures, metallic nanodot-encapsulated hollow mesoporous nanostructures have shown promising potential in various applications. However, their fabrication with versatile tunability of the encapsulated metallic content has been a challenge. Herein, we have prepared metallic nanodot-encapsulated hollow mesoporous silica nanoparticles (M-HMSNPs) with adjustable inner metallic components. The sacrificial template of polystyrene (PS) nanoparticles precoated with metals (Au/Ag/Pt) is fully wrapped with mesoporous silica (mSiO). The metallic nanodots are formed during the template removal process by calcination. The type and content of the encapsulated nanodots can be readily and precisely controlled by the initially deposited metallic layers. We demonstrate the application of the gold (Au) nanodot-loaded HMSNPs (denoted Au-HMSNPs) as smart surface-enhanced Raman spectroscopy (SERS) probes, which can screen between big molecules and small analytes. With the aid of a Raman reporter, the SERS probe can successfully quantify HO, which is used to distinguish cancer cells in vitro. Further integrated with enzymes, the SERS chips of specificity are prepared and used to detect corresponding substrates of glucose and uric acid, responsively. Besides SERS sensing, the current strategy can inspire future development of many other M-HMSNPs for various applications such as catalysis, energy storage, theranostics, etc.
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http://dx.doi.org/10.1021/acsami.0c17316DOI Listing
January 2021

Fundamentals and applications of enzyme powered micro/nano-motors.

Bioact Mater 2021 Jun 1;6(6):1727-1749. Epub 2020 Dec 1.

Flexible Printed Electronic Technology Center and School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen, 518055, China.

Micro/nanomotors (MNMs) are miniaturized machines that can convert many kinds of energy into mechanical motion. Over the past decades, a variety of driving mechanisms have been developed, which have greatly extended the application scenarios of MNMs. Enzymes exist in natural organisms which can convert chemical energy into mechanical force. It is an innovative attempt to utilize enzymes as biocatalyst providing driving force for MNMs. The fuels for enzymatic reactions are biofriendly as compared to traditional counterparts, which makes enzyme-powered micro/nanomotors (EMNMs) of great value in biomedical field for their nature of biocompatibility. Until now, EMNMs with various shapes can be propelled by catalase, urease and many others. Also, they can be endowed with multiple functionalities to accomplish on-demand tasks. Herein, combined with the development process of EMNMs, we are committed to present a comprehensive understanding of EMNMs, including their types, propelling principles, and potential applications. In this review, we will introduce single enzyme that can be used as motor, enzyme powered molecule motors and other micro/nano-architectures. The fundamental mechanism of energy conversion process of EMNMs and crucial factors that affect their movement behavior will be discussed. The current progress of proof-of-concept applications of EMNMs will also be elaborated in detail. At last, we will summarize and prospect the opportunities and challenges that EMNMs will face in their future development.
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http://dx.doi.org/10.1016/j.bioactmat.2020.11.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711193PMC
June 2021

RBMX contributes to hepatocellular carcinoma progression and sorafenib resistance by specifically binding and stabilizing BLACAT1.

Am J Cancer Res 2020 1;10(11):3644-3665. Epub 2020 Nov 1.

Department of Nuclear Medicine, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine 358 Datong Road, Pudong, Shanghai 200137, P. R. China.

Hepatocellular carcinoma (HCC) is one of the top five causes of cancer death. The interaction of RNA binding proteins and long no coding RNA play vital role in malignant tumor progression, and even contribute to chemoresistance. RNA binding protein X (RBMX) plays a vital role in binding and stabilizing many proteins. In this study, we have identified RBMX significantly contributes to the tumorigenesis and sorafenib resistance of hepatocellular carcinoma (HCC). We observed that RBMX was highly expressed in both the HCC patient tissues and HCC cell lines. The HCC cell's viability, proliferation, and sorafenib resistance ability were both increased when RBMX was overexpressed. Additional, RBMX also promotes HCC development and chemoresistance . Further, we found that the autophagy level was increased in HCC cells, which RBMX was up regulated, with sorafenib processing. Interestingly, our study found that long no coding RNA bladder cancer associated transcript 1 (LncBLACAT1) was also raised in HCC. Mechanically, RIP, RNA pull-down and RNA Stability assay proved that RBMX could specially binds BLACAT1's mRNA and matins its expression, which is high degree of consistency with database prediction. This mechanism of action is beneficial for cancer cells proliferation, anti-apoptotic, and colony formation with sorafenib treatment. Further, the autophagy level and cancer cell stemness were also improved when RBMX/BLACAT1 upregulated. Our study indicated that hepatoma cells can improve their proliferation, colony ability and autophagy by RBMX stabilizing BLACAT1 expression then promote HCC development and drug resistance. Hence, RBMX could be considered as novel therapeutic target for HCC treatment strategies.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716158PMC
November 2020

Wearable Circuits Sintered at Room Temperature Directly on the Skin Surface for Health Monitoring.

ACS Appl Mater Interfaces 2020 Oct 27;12(40):45504-45515. Epub 2020 Sep 27.

Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.

A soft body area sensor network presents a promising direction in wearable devices to integrate on-body sensors for physiological signal monitoring and flexible printed circuit boards (FPCBs) for signal conditioning/readout and wireless transmission. However, its realization currently relies on various sophisticated fabrication approaches such as lithography or direct printing on a carrier substrate before attaching to the body. Here, we report a universal fabrication scheme to enable printing and room-temperature sintering of the metal nanoparticle on paper/fabric for FPCBs and directly on the human skin for on-body sensors with a novel sintering aid layer. Consisting of polyvinyl alcohol (PVA) paste and nanoadditives in the water, the sintering aid layer reduces the sintering temperature. Together with the significantly decreased surface roughness, it allows for the integration of a submicron-thick conductive pattern with enhanced electromechanical performance. Various on-body sensors integrated with an FPCB to detect health conditions illustrate a system-level example.
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http://dx.doi.org/10.1021/acsami.0c11479DOI Listing
October 2020

Durability of Fibre-Reinforced Calcium Aluminate Cement (CAC)-Ground Granulated Blast Furnace Slag (GGBFS) Blended Mortar after Sulfuric Acid Attack.

Materials (Basel) 2020 Aug 29;13(17). Epub 2020 Aug 29.

UniSA STEM, University of South Australia, Adelaide 5095, Australia.

Concrete wastewater infrastructures are important to modern society but are susceptible to sulfuric acid attack when exposed to an aggressive environment. Fibre-reinforced mortar has been adopted as a promising coating and lining material for degraded reinforced concrete structures due to its unique crack control and excellent anti-corrosion ability. This paper aims to evaluate the performance of polyethylene (PE) fibre-reinforced calcium aluminate cement (CAC)-ground granulated blast furnace slag (GGBFS) blended strain-hardening mortar after sulfuric acid immersion, which represented the aggressive sewer environment. Specimens were exposed to 3% sulfuric acid solution for up to 112 days. Visual, physical and mechanical performance such as water absorption ability, sorptivity, compressive and direct tensile strength were evaluated before and after sulfuric acid attack. In addition, micro-structure changes to the samples after sulfuric acid attack were also assessed by X-Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM) to further understand the deterioration mechanism. The results show that overall fibre-reinforced calcium aluminate cement (CAC)-based samples performed significantly better than fibre-reinforced ordinary Portland cement (OPC)-based samples as well as mortar samples in sulfuric acid solution in regard to visual observations, penetration depth, direct tensile strength and compressive reduction. Gypsum generation in the cementitious matrix of both CAC and OPC-based systems was the main reason behind the deterioration mechanism after acid attack exposure. Moreover, laboratory sulfuric acid testing has been proven for successfully screening the cementitious material against an acidic environment. This method can be considered to design the service life of concrete wastewater pipes.
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http://dx.doi.org/10.3390/ma13173822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503559PMC
August 2020

Biomarkers detection with magnetoresistance-based sensors.

Biosens Bioelectron 2020 Oct 13;165:112340. Epub 2020 Jun 13.

School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, 611731, PR China. Electronic address:

Biosensing platforms for detecting and quantifying biomarkers have played an important role in the past decade. Among them, platforms based on magnetoresistance (MR) sensing technology are attractive. The resistance value of the material changes with the externally applied magnetic field is the core mechanism of MR sensing technology. A typical MR-based sensor has the characteristics of cost-effective, simple operation, high compactness, and high sensitivity. Moreover, using magnetic nanoparticles (MNPs) as labels, MR-based sensors have the ability to overcome the high background noise of complex samples, so they are particularly suitable for point-of-care testing (POCT). However, the problem still exists. How to obtain high-throughput, that is, multiple detections of biomarkers in MR-based sensors, thereby improving detection efficiency and reducing the burden on patients is an important issue in future work. This paper reviews three MR-based detection technologies for the detection of biomarkers, i.e., anisotropic magnetoresistance (AMR), giant magnetoresistance (GMR), and tunneling magnetoresistance (TMR). Based on these three common technologies, different typical applications that include biomedical diagnosis, food safety, and environmental monitoring are presented. Furthermore, the existing MR-based detection method is better expanded to make it more in line with present detection needs by combining different advanced technologies including microfluidics, Microelectromechanical systems (MEMS), and Immunochromatographic test strips (ICTS). And then, a brief discussion of current challenges and perspectives of MR-based sensors are pointed out.
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http://dx.doi.org/10.1016/j.bios.2020.112340DOI Listing
October 2020

GINS2 affects cell viability, cell apoptosis, and cell cycle progression of pancreatic cancer cells via MAPK/ERK pathway.

J Cancer 2020 19;11(16):4662-4670. Epub 2020 May 19.

Department of Nuclear Medicine, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China.

GINS complex subunit 2 (GINS2), a member of the GINS complex, is involved in DNA replication. GINS2 is upregulated in a variety of aggressive tumors, such as leukemia, breast cancer, and cervical cancer. However, the role of GINS2 in pancreatic cancer has still remained elusive. In this study, PANC-1 and BxPC-3 cell lines were chosen to perform experiments in vitro. Additionally, the effects of GINS2 interference on the cell viability, cell apoptosis, cell cycle, and tumor growth in nude mice were analyzed. We utilized pancreatic cancer cell lines that knocked down GINS2 expression using small interference RNA (siRNA) and evaluated GINS2 expression using Western blot analysis. To explore the function of GINS2 in pancreatic cancer cell lines in vitro, MTT assay and flow cytometry were used. Additionally, we investigated the potential mechanism of GINS2 interference by identifying the MAPK/ERK pathway using Western blotting. Finally, PANC-1 cells with GINS2 knockdown were subcutaneously injected into nude mice to evaluate the effects of GINS2 on tumor growth . It was unveiled that GINS2 interference inhibited cell viability, induced cell cycle arrest at G1 phase, and enhanced apoptosis of pancreatic cancer cell lines. Western blot assay indicated that GINS2 interference increased the expression level of Bax, while the expression level of Bcl-2 was remarkably decreased. In addition, the expression levels of CDK4, CDK6, and Cyclin D1 were significantly reduced after treatment with GINS2 siRNA. Furthermore, GINS2 interference drastically attenuated the expression levels of MEK, p-MEK, ERK, and p-ERK, belonging to the MAPK/ERK pathway. The results of an established cancer xenograft model revealed that nude mice transplanted with cells expressing negative control (NC) exhibited larger and heavier tumors, while volume and weight of tumor were remarkably reduced in ones transplanted with cells expressing GINS2 siRNA. GINS2 interference inhibited cell viability, induced cell cycle arrest, and promoted cell apoptosis of pancreatic cancer cell lines via the MAPK/ERK pathway, and our findings may be valuable for treating pancreatic cancer.
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http://dx.doi.org/10.7150/jca.38386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330693PMC
May 2020

Complete Genome Sequence of a Gram-Positive Bacterium, sp. Strain PS1209, a Potato Endophyte.

Microbiol Resour Announc 2020 Jun 25;9(26). Epub 2020 Jun 25.

School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, New York, USA.

We report the complete and annotated genome sequence of a Gram-positive bacterium, sp. strain PS1209, a potato endophyte that was isolated from apparently healthy tubers of potato cultivar NY166. The circular genome is 4,091,164 bp long, with a GC content of 69.08%, containing 3,926 genes.
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http://dx.doi.org/10.1128/MRA.00447-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317099PMC
June 2020

Biomedical Micro-/Nanomotors: From Overcoming Biological Barriers to In Vivo Imaging.

Adv Mater 2021 Feb 24;33(6):e2000512. Epub 2020 Jun 24.

Key Laboratory of Microsystems and Microstructures Manufacturing, Harbin Institute of Technology, 92 West Dazhi Street, Harbin, 150080, China.

Self-propelled micro- and nanomotors (MNMs) have shown great potential for applications in the biomedical field, such as active targeted delivery, detoxification, minimally invasive diagnostics, and nanosurgery, owing to their tiny size, autonomous motion, and navigation capacities. To enter the clinic, biomedical MNMs request the biodegradability of their manufacturing materials, the biocompatibility of chemical fuels or externally physical fields, the capability of overcoming various biological barriers (e.g., biofouling, blood flow, blood-brain barrier, cell membrane), and the in vivo visual positioning for autonomous navigation. Herein, the recent advances of synthetic MNMs in overcoming biological barriers and in vivo motion-tracking imaging techniques are highlighted. The challenges and future research priorities are also addressed. With continued attention and innovation, it is believed that, in the future, biomedical MNMs will pave the way to improve the targeted drug delivery efficiency.
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http://dx.doi.org/10.1002/adma.202000512DOI Listing
February 2021

Magnetic Nanomotor-Based Maneuverable SERS Probe.

Research (Wash D C) 2020 5;2020:7962024. Epub 2020 Jun 5.

Flexible Printed Electronic Technology Center and School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China.

Surface-enhanced Raman spectroscopy (SERS) is a powerful sensing technique capable of capturing ultrasensitive fingerprint signal of analytes with extremely low concentration. However, conventional SERS probes are passive nanoparticles which are usually massively applied for biochemical sensing, lacking controllability and adaptability for precise and targeted sensing at a small scale. Herein, we report a "rod-like" magnetic nanomotor-based SERS probe (MNM-SP) that integrates a mobile and controllable platform of micro-/nanomotors with a SERS sensing technique. The "rod-like" structure is prepared by coating a thin layer of silica onto the self-assembled magnetic nanoparticles. Afterwards, SERS hotspots of silver nanoparticles (AgNPs) are decorated as detecting nanoprobes. The MNM-SPs can be navigated on-demand to avoid obstacles and target sensing sites by the guidance of an external gradient magnetic field. Through applying a rotating magnetic field, the MNM-SPs can actively rotate to efficiently stir and mix surrounding fluid and thus contact with analytes quickly for SERS sensing. Innovatively, we demonstrate the self-cleaning capability of the MNM-SPs which can be used to overcome the contamination problem of traditional single-use SERS probes. Furthermore, the MNM-SPs could precisely approach the targeted single cell and then enter into the cell by endocytosis. It is worth mentioning that by the effective mixing of intracellular biocomponents, much more informative Raman signals with improved signal-to-noise ratio can be captured after active rotation. Therefore, the demonstrated magnetically activated MNM-SPs that are endowed with SERS sensing capability pave way to the future development of smart sensing probes with maneuverability for biochemical analysis at the micro-/nanoscale.
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http://dx.doi.org/10.34133/2020/7962024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293755PMC
June 2020

P4HB modulates epithelial-mesenchymal transition and the β-catenin/Snail pathway influencing chemoresistance in liver cancer cells.

Oncol Lett 2020 Jul 23;20(1):257-265. Epub 2020 Apr 23.

Department of Nuclear Medicine, The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.

The aim of the present study was to investigate the role of prolyl 4-hydroxylase beta polypeptide (P4HB) in the chemoresistance of liver cancer. Drug-resistant liver cancer cell lines, such as HepG2/adriamycin (ADR) cells, were treated and screened using adriamycin. Gene interference was used to silence the expression of in liver cancer cells. Cell viability, invasiveness and migration were assessed using CCK8, Transwell and wound healing assays, respectively. In addition, changes to key genes and proteins in the epithelial-mesenchymal transition (EMT) and β-catenin/Snail pathway were analyzed using reverse transcription-quantitative PCR and western blotting. Drug-resistant HepG2/ADR cells were successfully cultivated; the IC to ADR for HepG2/ADR and HepG2 cell lines was 4.85 and 0.61 µM, respectively. HepG2/ADR cells exhibited higher invasion and migration abilities compared with HepG2 cells (P<0.05). E-cadherin mRNA and protein expression levels in HepG2/ADR cells were decreased significantly, whereas P4HB, N-cadherin and vimentin mRNA and protein levels were significantly increased compared with HepG2 cells (all P<0.05). Knockdown of P4HB significantly decreased cell viability and the invasion and migration ability of HepG2/ADR cells. In addition, P4HB knockdown enhanced E-cadherin mRNA and protein expression levels, whereas N-cadherin, vimentin, total β-catenin, nuclear β-catenin and Snail mRNA and protein levels were significantly decreased (all P<0.05). Overall, the present study demonstrated that EMT and β-catenin/Snail pathway influence P4HB modulation in liver cancer chemoresistance.
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http://dx.doi.org/10.3892/ol.2020.11569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285890PMC
July 2020

Ginkgo biloba extract protects diabetic rats against cerebral ischemia‑reperfusion injury by suppressing oxidative stress and upregulating the expression of glutamate transporter 1.

Mol Med Rep 2020 Apr 19;21(4):1809-1818. Epub 2020 Feb 19.

Department of Pharmacology, School of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China.

The current study aimed to evaluate the neuroprotective effect of Ginkgo biloba extract (GbE) on the progression of acute cerebral ischemia‑reperfusion injury in diabetic rats, and to determine the molecular mechanism associated with this effect. Streptozotocin (STZ) induced diabetic rats were pretreated with GbE (50, 100 and 200 mg/kg/day; intragastric) for 3 weeks. During this period, body weight changes and fasting blood glucose levels were assessed each week. Following pretreatment, rats were subjected to suture occlusion of the middle cerebral artery for 30 min, which was followed by 24 h of reperfusion. Neurological deficits were subsequently evaluated at 2 and 24 h following reperfusion. Rats were sacrificed after 24 h reperfusion, and infarct volume and S100B content were measured to evaluate the neuroprotective effect of GbE. The results of the present study demonstrated that GbE pretreatment improved neurological scores, and reduced cerebral infarct volume and S100B content. Oxidative stress markers, including glutathione (GSH) and superoxide dismutase (SOD) were increased, and malondialdehyde (MDA) contents were reduced following GbE treatment. The levels of p‑Akt, p‑mTOR and glutamate transporter 1 (GLT1) were observed to be increased in GbE‑pretreated rats. These results indicated that GbE pretreatment may serve a protective role against cerebral ischemia‑reperfusion injury in diabetic rats by inhibiting oxidative stress reaction, upregulating the expression of Akt/mTOR and promoting GLT1 expression. In conclusion, the current study revealed the protective role and molecular mechanisms of GbE in diabetic rats with cerebral ischemia‑reperfusion injury, and may provide novel insight into the future clinical treatment of this condition.
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http://dx.doi.org/10.3892/mmr.2020.10990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057817PMC
April 2020

Bimetallic coatings synergistically enhance the speeds of photocatalytic TiO micromotors.

Chem Commun (Camb) 2020 Apr 29;56(34):4728-4731. Epub 2020 Mar 29.

School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong 518055, China.

The design of powerful, more biocompatible microrobots calls for faster catalytic reactions. Here we demonstrate a two-fold increase in the speed of photocatalytic TiO-metal Janus micromotors via a Au/Ag bi-layered coating. Electrochemical measurements show that such a bimetallic coating is a better photocatalyst than either metal alone. Similarly, an additional sputtered Ag layer could also significantly increase the speed of Pt-PS or TiO-Pt micromotors, suggesting that applying bimetallic coatings is a generalizable strategy in the design of faster catalytic micromotors.
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http://dx.doi.org/10.1039/d0cc00212gDOI Listing
April 2020

Multiplexed detection of biomarkers in lateral-flow immunoassays.

Analyst 2020 Apr 27;145(8):2828-2840. Epub 2020 Mar 27.

School of Automation Engineering, University of Electronic Science and Technology of China, Chengdu 611731, P. R. China.

Multiplexed detection of biomarkers, i.e., simultaneous detection of multiple biomarkers in a single assay, is a process of great advantages including enhanced diagnostic precision, improved diagnostic efficiency, reduced diagnostic cost, and alleviated pain of patients. A typical lateral-flow immunoassay (LFIA) is a widely used paper-based immunochromatographic test strip designed to detect a target biomarker through two common formats: sandwich assay and competitive assay. In order to obtain qualitative or quantitative results, a probe with unique optical or magnetic properties is usually employed to characterize the concentration of the target biomarker. The typical LFIA is suitable for point-of-care testing due to its simplicity, portability, cost-effectiveness, and rapid detection of a target biomarker. However, detection of a single biomarker in the typical LFIA is not favorable for high throughput analysis. Therefore, multiplexed detection of biomarkers in LFIAs has been extensively studied in recent years for high throughput analysis. To accomplish multiplexed detection of biomarkers in LFIAs, the most frequently used structure is a test strip with multiple test lines (TLs), where each TL can detect a specific biomarker. An alternative structure, i.e., a multi-channel structure with multiple test strips, where each test strip has one TL for detecting a specific biomarker, is employed for multiplexed detection of biomarkers. Sometimes, a single test strip with only one TL containing different receptors, where each detection receptor corresponds to a specific biomarker, is another structure applied for multiplexed detection of biomarkers. This paper reviews three common structures for multiplexed detection of biomarkers in LFIAs, i.e., a test strip with multiple TLs, a multi-channel structure with multiple test strips, and a test strip with a single TL. Based on the three common structures, different signal detection strategies that include colorimetric detection, fluorescence detection, surface-enhanced Raman scattering detection, and magnetic detection, along with performance and perspectives are discussed.
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http://dx.doi.org/10.1039/c9an02485aDOI Listing
April 2020

Protective effect of brain-derived neurotrophic factor and neurotrophin-3 overexpression by adipose-derived stem cells combined with silk fibroin/chitosan scaffold in spinal cord injury.

Neurol Res 2020 May 9;42(5):361-371. Epub 2020 Mar 9.

Department of Orthopaedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

: Spinal cord injury (SCI) is a most debilitating traumatic injury, and cytotherapy is a promising alternative treatment strategy. Here we investigated the effect and mechanism of adipose-derived stem/stromal cells (ASCs) with overexpressing brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT3) (BDNF-NT3) in combination with silk fibroin/chitosan scaffold (SFCS) in SCI.: Female Sprague-Dawley rats were used as an SCI model. SFCS,SFCS and ASCs, or ASCs overexpressing NT3, BDNF, and BDNF-NT3 were implanted into SCI rats. Basso, Beattie, and Bresnahan score, pathological changes, and spinal cord tissue and nerve fiber morphology were observed and assayed. GAP-43, GFAP, and caspase-3 expression was determined using immunohistochemistry and western blotting.: Smoother spinal cords, less scar tissue, and lower inflammatory activity were found in the SFCS, SFCS and ASCs, ASCs with NT3, BDNF, and BDNF-NT3 overexpression treatment than in the untreated SCI rat groups. Increasing formation of nerve fibers was observed in the above groups in order. GAP-43 expression significantly increased, while GFAP and caspase-3 expression significantly decreased. These results indicated obvious alleviation in pathological changes and BDNF-NT3 overexpression in ASCs combined with SFCS treatment in SCI rats.: Thus, BDNF-NT3 overexpression from ASCs with SFCS had synergistic neuroprotective effects on SCI and may be a treatment option for SCI.
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http://dx.doi.org/10.1080/01616412.2020.1735819DOI Listing
May 2020

Photoacoustic Imaging-Trackable Magnetic Microswimmers for Pathogenic Bacterial Infection Treatment.

ACS Nano 2020 03 10;14(3):2880-2893. Epub 2020 Mar 10.

Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, United States.

Micro/nanorobots have been extensively explored as a tetherless small-scale robotic biodevice to perform minimally invasive interventions in hard-to-reach regions. Despite the emergence of versatile micro/nanorobots in recent years, matched development remains challenging, limited by unsatisfactory integration of core functions. Herein, we report a polydopamine (PDA)-coated magnetic microswimmer consisting of a magnetized (MSP) matrix and PDA surface. Apart from the properties of the existing MSP (, robust propulsion, natural fluorescence, tailored biodegradation, and selective cytotoxicity), the introduced PDA coating enhances the photoacoustic (PA) signal and photothermal effect of the MSP, thus making PA image tracking and photothermal therapy possible. Meanwhile, the PDA's innate fluorescence quenching and diverse surface reactivity allows an off-on fluorescence diagnosis with fluorescence probes (, coumarin 7). As a proof of concept, real-time image tracking (by PA imaging) and desired theranostic capabilities of PDA-MSP microswimmer swarms are demonstrated for the treatment of pathogenic bacterial infection. Our study suggests a feasible antibacterial microrobot for development and a facile yet versatile functionalization strategy of micro/nanorobots.
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http://dx.doi.org/10.1021/acsnano.9b06731DOI Listing
March 2020