Publications by authors named "Xing Liu"

1,437 Publications

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Extended En Bloc Reoperation for Recurrent or Persistent Parathyroid Carcinoma: Analysis of 31 Cases in a Single Institute Experience.

Ann Surg Oncol 2021 Oct 25. Epub 2021 Oct 25.

Department of Thyroid and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, China.

Background: Parathyroid carcinoma (PC) is a rare malignancy that is difficult to eradicate completely after recurrence. We assessed the efficiency of extended en bloc resection (EEBR) in the management of recurrent or persistent PC.

Methods: In this observational cohort study, 31 patients who underwent reoperations for recurrent or persistent PC were enrolled after 2-9 surgeries by other medical teams. EEBRs, which provided the oncologic resection by removing all possible tumor-bearing scar tissues, were adopted in 25 patients. The other 6 with gross infiltration into the upper aerodigestive tract (UAT) underwent less radical procedures for unwillingness to sacrifice laryngeal function.

Results: The 5-year overall survival (OS) rate after EEBR was 59.6% compared with 16.7% after less radical procedures, with an improved median expected survival time of 90.0 months compared with 13.0 months after local excision. EEBR exhibited a favorable local control of relapses in 84.0% of patients after a median follow-up period of 27.0 months, 40.0% even achieved disease-free survival, although 56.0% had subsequent distant metastases (DMs) and suffered a worse 5-year OS of 36.7% in contrast with 100.0% in the absence of DM (p = 0.011). UAT invasion, rather than age, number of previous operations, or preoperative PTH levels, was the unique independent factor associated with both DM (HR = 5.466, p = 0.006) and mortality (HR = 7.606, p = 0.011).

Conclusion: EEBRs provide better outcomes than other conventional surgical approaches and might offer a second chance of cure for patients with recurrent or persistent PC in the absence of DM.
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http://dx.doi.org/10.1245/s10434-021-10962-7DOI Listing
October 2021

Novel 2D CaCl crystals with metallicity, room-temperature ferromagnetism, heterojunction, piezoelectricity-like property and monovalent calcium ions.

Natl Sci Rev 2021 Jul 7;8(7):nwaa274. Epub 2020 Nov 7.

Department of Physics, East China University of Science and Technology, Shanghai 200237, China.

Under ambient conditions, the only known valence state of calcium ions is +2, and the corresponding crystals with calcium ions are insulating and nonferromagnetic. Here, using cryo-electron microscopy, we report direct observation of two-dimensional (2D) CaCl crystals on reduced graphene oxide (rGO) membranes, in which the calcium ions are only monovalent (i.e. +1). Remarkably, metallic rather than insulating properties are displayed by those CaCl crystals. More interestingly, room-temperature ferromagnetism, graphene-CaCl heterojunction, coexistence of piezoelectricity-like property and metallicity, as well as the distinct hydrogen storage and release capability of the CaCl crystals in rGO membranes are experimentally demonstrated. We note that such CaCl crystals are obtained by simply incubating rGO membranes in salt solutions below the saturated concentration, under ambient conditions. Theoretical studies suggest that the formation of those abnormal crystals is attributed to the strong cation-π interactions of the Ca cations with the aromatic rings in the graphene surfaces. The findings highlight the realistic potential applications of such abnormal CaCl material with unusual electronic properties in designing novel transistors and magnetic devices, hydrogen storage, catalyzers, high-performance conducting electrodes and sensors, with a size down to atomic scale.
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http://dx.doi.org/10.1093/nsr/nwaa274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310769PMC
July 2021

Association of Obesity with the Risk of Hyperhomocysteinemia among the Chinese Community Residents: A Prospective Cohort Study in Shanghai, China.

Nutrients 2021 Oct 18;13(10). Epub 2021 Oct 18.

The Key Laboratory of Public Health Safety of Ministry of Education, Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China.

A prospective community-based cohort study was conducted to investigate the effects of obesity on hyperhomocysteinemia (HHcy) in community residents from Shanghai, China, with a median follow-up period of 2.98 years. The exposures were high body mass index (BMI) (BMI ≥ 28.0 kg/m) and high waist circumference (WC) (WC ≥ 85.0 cm for female and WC ≥ 90.0 for male) at baseline investigation, and the outcome was the incident of HHcy after the follow-up. A restricted cubic spline (RCS) was performed to assess the possible nonlinear relationship of BMI and WC with HHcy. A Cox proportional hazard regression model was used to evaluate the association between BMI and WC measured obesity and the risk of HHcy (Hcy level > 15 µmol/L). No significant non-linearity was found between BMI and WC with HHcy. Cox regression model showed that underweight measured by BMI was negatively associated with the risk of HHcy after controlling for confounder variables (adjusted HR = 0.64, 95% CI = 0.42 to 0.99). While abdominal obesity was positively associated with the risk of HHcy for those without CVD-related comorbidities (adjusted HR = 1.26, 95% CI = 1.05 to 1.51). Our results suggested that individuals could maintain a relatively low BMI and normal WC to lower the risk of HHcy.
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http://dx.doi.org/10.3390/nu13103648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537264PMC
October 2021

Long Non-coding RNAs and Circular RNAs: Insights Into Microglia and Astrocyte Mediated Neurological Diseases.

Front Mol Neurosci 2021 5;14:745066. Epub 2021 Oct 5.

Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

Microglia and astrocytes maintain tissue homeostasis in the nervous system. Both microglia and astrocytes have pro-inflammatory phenotype and anti-inflammatory phenotype. Activated microglia and activated astrocytes can contribute to several neurological diseases. Long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), two groups of non-coding RNAs (ncRNAs), can function as competing endogenous RNAs (ceRNAs) to impair the microRNA (miRNA) inhibition on targeted messenger RNAs (mRNAs). LncRNAs and circRNAs are involved in various neurological disorders. In this review, we summarized that lncRNAs and circRNAs participate in microglia dysfunction, astrocyte dysfunction, neuron damage, and inflammation. Thereby, lncRNAs and circRNAs can positively or negatively regulate neurological diseases, including spinal cord injury (SCI), traumatic brain injury (TBI), ischemia-reperfusion injury (IRI), stroke, neuropathic pain, epilepsy, Parkinson's disease (PD), multiple sclerosis (MS), and Alzheimer's disease (AD). Besides, we also found a lncRNA/circRNA-miRNA-mRNA regulatory network in microglia and astrocyte mediated neurological diseases. Through this review, we hope to cast light on the regulatory mechanisms of lncRNAs and circRNAs in microglia and astrocyte mediated neurological diseases and provide new insights for neurological disease treatment.
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http://dx.doi.org/10.3389/fnmol.2021.745066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523841PMC
October 2021

Clinical analysis of metastatic characteristics of infrapyloric lymph nodes (No.206) and terminal ileum lymph nodes in patients with right colon cancer.

World J Surg Oncol 2021 Oct 22;19(1):310. Epub 2021 Oct 22.

Department of Colorectal Surgery, the First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Fuzhou, 350000, Fujian, China.

Background: D3 or complete mesocolic excision (CME) surgery has become a common surgical procedure for the treatment of colon cancer metastasis. Clinical misuse and overuse of lymph node dissection bring unnecessary burdens to patients. A detailed guidance for lymph node dissection in patients with T3 and T4 stage right colon cancer at different locations is urgently needed.

Methods: A retrospective study was performed. Patients received D3 or CME surgery were divided into ileocecal group, ascending colon group, and hepatic flexure group according to the 9th edition of the Japanese Society for Cancer of the Colon and Rectum guidelines. The distributions of lymph node metastases were analyzed according to tumor infiltration depth (T stage) and tumor location.

Results: The incidence of metastases in the paracolic area (or station), intermediate area, and main (or central) area was 38.4% (139/362), 12.7% (46/362), and 9.7% (35/362), respectively. The proportion of patients having No.206 and terminal ileum lymph nodes metastases was 7.7% (14/181) and 3.7% (9/244), respectively. No.206 lymph node metastasis is related to tumor location (χ = 7.955, p = 0.019) and degree of differentiation (χ = 18.99, p = 0.000), and terminal ileum lymph node metastasis is related to tumor location (χ = 6.273, p = 0.043). Patients with T3/T4 hepatic flexure cancer received radical right hemicolectomy in addition to No.206 lymph node dissection.

Conclusion: Radical right hemicolectomy and No.206 group lymph node dissection are necessary for T3 and T4 stage colon cancer therapy.
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http://dx.doi.org/10.1186/s12957-021-02414-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532261PMC
October 2021

-Doping of Graphene Aerogel as a Multifunctional Air Cathode for Microbial Fuel Cells.

ACS Appl Mater Interfaces 2021 Oct 21. Epub 2021 Oct 21.

College of Marine Science-Technology and Environment, Dalian Ocean University, No. 52 Heishijiao, Shahekou District, Dalian 116023, P.R. China.

One of the main challenges faced by microbial fuel cells (MFCs) generating voltage is how to facilitate the oxygen reduction reaction (ORR) process using a specifically designed air cathode, especially by optimizing a three-phase catalytic interface and enhanced O diffusion on it. Herein, a three-dimensional porous -doped graphene aerogel (NGA) is polymerized onto a steel mesh (SM) to construct a simple structure of an air cathode (NGA-x/SM) hydrothermal synthesis and subsequent freeze-drying treatment; more specifically, NGA was simultaneously used as an efficient ORR catalyst layer and breathable gas diffusion layer to improve the performance of MFCs. In this system, the NGA-5/SM (with a precursor concentration of = 5.0 mg mL) makes itself a perfect candidate to be used as an air cathode. Characterization parameters reveal that sub-micrometer micropores, defective multilayer structures, and the highest proportion of pyridinic-N (48.1%) exist in NGA-5/SM. Furthermore, electrochemical measurements demonstrate that it has an oxygen reduction peak potential of 0.63 V, a Tafel slope of 187 mV dec, and closest 4e transfer pathway ( = 3.2-3.5). These data prove that a three-phase boundary can naturally form in NGA-5/SM, where the ORR occurs. More importantly, this work provides a proof of concept that a Pt-free air cathode could be prepared with high-efficiency NGA by a two-step preparation method to achieve a MFC maximum power density of 1593 mW m.
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http://dx.doi.org/10.1021/acsami.1c12605DOI Listing
October 2021

Versatile Types of Inorganic/Organic NIR-IIa/IIb Fluorophores: From Strategic Design toward Molecular Imaging and Theranostics.

Chem Rev 2021 Oct 19. Epub 2021 Oct 19.

State Key Laboratory of Virology, College of Science, Research Center for Ecology, Laboratory of Extreme Environmental Biological Resources and Adaptive Evolution, Tibet University, Lhasa 850000, China.

imaging in the second near-infrared window (NIR-II, 1000-1700 nm), which enables us to look deeply into living subjects, is producing marvelous opportunities for biomedical research and clinical applications. Very recently, there has been an upsurge of interdisciplinary studies focusing on developing versatile types of inorganic/organic fluorophores that can be used for noninvasive NIR-IIa/IIb imaging (NIR-IIa, 1300-1400 nm; NIR-IIb, 1500-1700 nm) with near-zero tissue autofluorescence and deeper tissue penetration. This review provides an overview of the reports published to date on the design, properties, molecular imaging, and theranostics of inorganic/organic NIR-IIa/IIb fluorophores. First, we summarize the design concepts of the up-to-date functional NIR-IIa/IIb biomaterials, in the order of single-walled carbon nanotubes (SWCNTs), quantum dots (QDs), rare-earth-doped nanoparticles (RENPs), and organic fluorophores (OFs). Then, these novel imaging modalities and versatile biomedical applications brought by these superior fluorescent properties are reviewed. Finally, challenges and perspectives for future clinical translation, aiming at boosting the clinical application progress of NIR-IIa and NIR-IIb imaging technology are highlighted.
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http://dx.doi.org/10.1021/acs.chemrev.1c00553DOI Listing
October 2021

[Effects of biochar application patterns on soil nutrients and nitrogen- and phosphorus-related enzyme activities in Phaeozem and Luvisol].

Ying Yong Sheng Tai Xue Bao 2021 Aug;32(8):2693-2702

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

We investigated the effects of different biochar application patterns on soil nutrient contents and element transformation, with soil samples being collected from two five-year field experiments in Phaeozem and Luvisol amended with biochar at annual low-rate (AL, 22.5 t·hm·a) and intervalic high-rate (IH, 112.5 t·hm·5 a). Changes of soil total carbon (C), nitrogen (N) and phosphorus (P) contents as well as the related enzyme activities were measured under different biochar application patterns to provide fundamental information for the straw utilization and soil fertility improvement in agroecosystem. Results showed that total C and organic N contents in AL treatment were significantly higher than those in IH treatment in Phaeozem soil. Compared with the control, the decreases of dehydrogenase activity in AL treatment was more pronounced than that in IH treatment in Phaeozem soil, and the increases of protease activity in IH treatment was pronounced than that in AL treatment in Luvisol. Compared with Luvisol soil, the application of biochar had stronger effect on total soil C and organic N contents in Phaeozem soil. Application of biochar significantly increased the activities of soil dehydrogenase and protease in Luvisol soil, but decreased the activity of soil dehydrogenase. Soil types and biochar application patterns interacted to affect soil C and N contents, microbial metabolic activity, N- and P-related enzyme activities. In summary, soil types and biochar addition affected soil properties and microbial characteristics, which would provide important information for straw application and soil management.
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http://dx.doi.org/10.13287/j.1001-9332.202108.041DOI Listing
August 2021

Astrocytes in Neural Circuits: Key Factors in Synaptic Regulation and Potential Targets for Neurodevelopmental Disorders.

Front Mol Neurosci 2021 29;14:729273. Epub 2021 Sep 29.

Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

Astrocytes are the major glial cells in the brain, which play a supporting role in the energy and nutritional supply of neurons. They were initially regarded as passive space-filling cells, but the latest progress in the study of the development and function of astrocytes highlights their active roles in regulating synaptic transmission, formation, and plasticity. In the concept of "tripartite synapse," the bidirectional influence between astrocytes and neurons, in addition to their steady-state and supporting function, suggests that any negative changes in the structure or function of astrocytes will affect the activity of neurons, leading to neurodevelopmental disorders. The role of astrocytes in the pathophysiology of various neurological and psychiatric disorders caused by synaptic defects is increasingly appreciated. Understanding the roles of astrocytes in regulating synaptic development and the plasticity of neural circuits could help provide new treatments for these diseases.
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http://dx.doi.org/10.3389/fnmol.2021.729273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515196PMC
September 2021

Zebrafish Attenuates Antiviral Innate Immunity by Targeting traf6.

J Immunol 2021 Oct 15. Epub 2021 Oct 15.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China;

TNFR-associated factor 6 (TRAF6) not only recruits TBK1/IKKε to MAVS upon virus infection but also catalyzes K63-linked polyubiquitination on substrate or itself, which is critical for NEMO-dependent and -independent TBK1/IKKε activation, leading to the production of type I IFNs. The regulation at the TRAF6 level could affect the activation of antiviral innate immunity. In this study, we demonstrate that zebrafish , a type I arginine methyltransferase, attenuates traf6-mediated antiviral response. Prmt2 binds to the C terminus of traf6 to catalyze arginine asymmetric dimethylation of traf6 at arginine 100, preventing its K63-linked autoubiquitination, which results in the suppression of traf6 activation. In addition, it seems that the N terminus of prmt2 competes with mavs for traf6 binding and prevents the recruitment of tbk1/ikkε to mavs. By zebrafish model, we show that loss of promotes the survival ratio of zebrafish larvae after challenge with spring viremia of carp virus. Therefore, we reveal, to our knowledge, a novel function of prmt2 in the negative regulation of antiviral innate immunity by targeting traf6.
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http://dx.doi.org/10.4049/jimmunol.2100627DOI Listing
October 2021

Repression of p53 function by SIRT5-mediated desuccinylation at Lysine 120 in response to DNA damage.

Cell Death Differ 2021 Oct 12. Epub 2021 Oct 12.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, P. R. China.

p53 is a classic tumor suppressor that functions in maintaining genome stability by inducing either cell arrest for damage repair or cell apoptosis to eliminate damaged cells in response to different types of stress. Posttranslational modifications (PTMs) of p53 are thought to be the most effective way for modulating of p53 activation. Here, we show that SIRT5 interacts with p53 and suppresses its transcriptional activity. Using mass spectrometric analysis, we identify a previously unknown PTM of p53, namely, succinylation of p53 at Lysine 120 (K120). SIRT5 mediates desuccinylation of p53 at K120, resulting in the suppression of p53 activation. Moreover, using double knockout mice (p53Sirt5), we validate that the suppression of p53 target gene expression and cell apoptosis upon DNA damage is dependent on cellular p53. Our study identifies a novel PTM of p53 that regulates its activation as well as reveals a new target of SIRT5 acting as a desuccinylase.
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http://dx.doi.org/10.1038/s41418-021-00886-wDOI Listing
October 2021

Association of the Reproductive Period with Decreased Estimated Glomerular Filtration Rate in Menopausal Women: A Study from the Shanghai Suburban Adult Cohort and Biobank (2016-2020).

Int J Environ Res Public Health 2021 10 5;18(19). Epub 2021 Oct 5.

Key Laboratory of Public Health Safety of Ministry of Education, School of Public Health, Fudan University, Shanghai 200032, China.

In previous studies, it has been documented that a short reproductive period is associated with a higher risk of diabetes, cardiovascular disease, and chronic kidney disease. This study aims to investigate the association of the reproductive period length with decreased renal function. This study obtained data from "the Shanghai Suburban Adult Cohort and Biobank". An estimated glomerular filtration rate (eGFR) below 60 mL/min/1.73 m indicated decreased renal function during follow-up. Participants were grouped into quintiles by reproductive period. Logistic regression analysis was performed to examine the association between the reproductive period and decreased renal function. A total of 5503 menopausal women with baseline eGFR > 60 mL/min/1.73 m were included. Age, eGFR, and metabolic equivalent of task (MET) at baseline were 61.0 (range, 36.0-74.0) years, 92.2 (range, 60.1-194.5) mL/min/1.73 m, and 1386 (range, 160-6678), respectively. A reproductive period of 37-45 years was associated with a lower risk of decreased eGFR (OR: 0.59, 95% CI: 0.35-1.00, = 0.049) after adjusting for confounding variables. METs decreased the risk of decreased eGFR in women with a reproductive period of 37-45 years (OR: 0.43, 95% CI: 0.23-0.81, = 0.010). Women with a longer reproductive period have a lower risk of decreased renal function. METs had an opposite influence on renal function in women with longer (decreased risk) or shorter (increased risk) reproductive periods.
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http://dx.doi.org/10.3390/ijerph181910451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508464PMC
October 2021

Transcriptome sequencing analysis of porcine alveolar macrophages infected with PRRSV strains to elucidate virus pathogenicity and immune evasion strategies.

Virusdisease 2021 Sep 31;32(3):559-567. Epub 2021 Jul 31.

College of Veterinary Medicine, Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, Inner Mongolia Agricultural University, No. 29, E'erduosi east street, Saihan Region, Hohhot, People's Republic of China.

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) causes a serious disease to the swine industry worldwide. To understand the mechanisms of HP-PRRSV infection, RNA-seq-based transcriptome analyses were performed on porcine alveolar macrophages (PAMs) infected with a HP-PRRSV strain (TJ), a less virulent strain of a classical lineage (CH-1a), and a vaccine strain TJM-F92. Gene ontology, Kyoto Encyclopedia of Genes and Genomes analyses indicate that TJM-F92 led to significant up-regulation of gene expression for proteins associated with membrane-bound organelles. The differentially expressed genes of HP-PRRSV TJ-infected PAM cells were up-regulated in the special G-protein coupled receptor. The six cytokines were tested by real time Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The relative expression levels showed the same trend of expression difference. Significant up-regulation of TMEM173 plays an important role in the cytosolic DNA-sensing pathway and the RIG-I-like receptor signaling pathway in TJM-F92 infected PAM cells. These data provide new insight into PRRSV pathogenicity and immune evasion strategies.
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http://dx.doi.org/10.1007/s13337-021-00724-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473512PMC
September 2021

Hyperinsulinemia negatively affects the association between insulin resistance and blood pressure.

Nutr Metab Cardiovasc Dis 2021 Aug 14. Epub 2021 Aug 14.

Hangzhou Aeronautical Sanatorium for Special Service of China Air Force, No. 27, Yang Gong Di, Xihu District, Hangzhou, Zhejiang, 310007, China. Electronic address:

Background And Aims: Although hyperinsulinemia and insulin resistance (IR) together cause metabolic diseases, the available evidence fails to link hyperinsulinemia with blood pressure (BP) elevation. To further understand the role of hyperinsulinemia in the pathophysiology of hypertension, we conducted this study to investigate the moderating effect of fasting insulin (FINS) on the association between IR and BP.

Methods And Results: The health screening data of 72,076 individuals were analyzed for this moderation analysis. IR was indicated by the homeostatic model assessment of insulin resistance (HOMA-IR), triglyceride-glucose index (TyG), and triglyceride to high-density lipoprotein cholesterol ratio (TG/HDLc). In the adjusted model, three IR indicators were considered independent variables; FINS was used as a moderator, and systolic BP (SBP) and diastolic BP (DBP) were used as dependent variables. The regression coefficient of the interaction term between the three IR indicators and FINS was significantly negative in all moderation models. Simple slope tests and the Johnson-Neymann technique also indicated that FINS negatively moderated the association between IR and BP.

Conclusions: This moderation analysis showed that FINS negatively mediated the association between IR and BP, suggesting that hyperinsulinemia may buffer, not reinforce, the effect of IR on hypertension.
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http://dx.doi.org/10.1016/j.numecd.2021.08.029DOI Listing
August 2021

A series of organic hybrid polyoxovanadate clusters incorporating tris(hydroxymethyl)methane derivatives.

Dalton Trans 2021 Oct 8. Epub 2021 Oct 8.

Chongqing Key Laboratory of Inorganic Functional Materials, College of Chemistry, Chongqing Normal University, Chongqing 401331, P. R. China.

A series of new organic hybrid polyoxovanadate clusters [VO(μ-OH)(acac)(Htri)] (1, Htri = tris(hydroxymethyl) aminomethane, acac = acetylacetone), [VO(acac)(Htri)(L)] {HL = methanol (2), ethanol (3a and 3b), ethylene glycol (4) and benzyl alcohol (5)}, {VO(HO)(tri-acetamide)(CHCOO)} (6, Htri-acetamide = -(2-hydroxy-1,1-bis-hydroxymethyl-ethyl)-acetamide), [VO(μ-OH)(Htri)]·6HO (7) and [VO(tri)(Htri)(HCOO)(CHCOO)]·2HO (8) were prepared by hydro(solvo)thermal methods and characterized structurally. 1 contains [VO(OH)(acac)] and [VO(Htri)] units, which are further interconnected common edges to build a tetravanadyl cluster [VO(OH)(acac)(Htri)] with the double-deficient cube [VO]. The tetravanadyl cluster frameworks of 2-5 can be derived from the tetravanadyl cluster of 1 by replacing two -OH groups with two deprotonated organic alcohol ligands, namely, CHO (2), CHCHO (3a and 3b), HO(CH)O (4) and CHCHO (5). Interestingly, both 3a and 3b have the same chemical structure, but they exhibit different conformational polymorphisms [denoted as α-type (3a) and β-type (3b)]. Such conformational polymorphisms within the polyoxovanadate clusters incorporating tris(hydroxymethyl)methane derivatives emerged for the first time. 6 displays another tetravanadyl cluster {VO(HO)(tri-acetamide)(CHCOO)} with a [VO] fragment, where the tri-acetamide unit comes from the amidation reaction of Htri and acetic acid and caps the tetrahedral void of the tetravanadyl cluster. The polyoxovanadate cluster of 7 can originate from the Lindqvist-type hexavanadyl cluster [VO] by replacing nine μ-oxides with nine alkoxides of three tri-acetamide ligands. 8 exhibits a fully reduced tetradecavanadyl cluster based on the linkage of two heptavanadyl clusters two O bridges. The magnetic properties of 1-8 show typical antiferromagnetic interactions.
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http://dx.doi.org/10.1039/d1dt02912fDOI Listing
October 2021

Identification of mRNA Prognostic Markers for TGCT by Integration of Co-Expression and CeRNA Network.

Front Endocrinol (Lausanne) 2021 21;12:743155. Epub 2021 Sep 21.

National Health Commission Key Laboratory of Human Stem Cell and Reproductive Engineering, Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, China.

Testicular germ cell tumor (TGCT) is the most common malignant tumor in young men and is associated with poor prognosis. We assessed the RNA expression profiles of 13 TGCT tissues and 4 adjacent normal tissues by transcriptome sequencing to identify novel prognostic biomarkers. We detected several differentially expressed mRNAs in TGCT that were functionally annotated by GO and KEGG enrichment analyses to tumorigenesis-related processes such as immunity and chemotherapeutic resistance. An mRNA-lncRNA-miRNA regulatory network was constructed using RNA-Seq data and public databases, and integrated with TCGA database to develop a prediction model for metastasis and recurrence. Finally, GRK4, PCYT2 and RGSL1 were identified as predictive markers of survival and therapeutic response. In conclusion, we found several potential predictors for TGCT prognosis and immunotherapeutic response by ceRNA network analysis.
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http://dx.doi.org/10.3389/fendo.2021.743155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491582PMC
September 2021

Gait characteristics and effects of the cerebrospinal fluid tap test in probable idiopathic normal pressure hydrocephalus.

Clin Neurol Neurosurg 2021 Sep 20;210:106952. Epub 2021 Sep 20.

Department of Neurology, Aviation General Hospital, 100012 Beijing, China. Electronic address:

Objectives: To evaluate gait characteristics and investigate changes pre- and post- cerebrospinal fluid tap test (CSF TT) in gait parameters in patients with probable idiopathic normal pressure hydrocephalus (iNPH).

Methods: Sixty patients were sequential circuited and diagnosed with possible iNPH according to Japanese second iNPH guidelines at our hospital from December 2016 to March 2021. All patients underwent the CSF TT. Gait parameters, cognitive and urinary function were assessed pre- and post-CSF TT. Patients who were unable to ambulate to take the tests or could not walk independently or walked normally were excluded.

Results: Twenty-six patients were diagnosed with probable iNPH using the CSF TT. After CSF TT, the Boon sum score improved from 20.0 ± 7.7-16.6 ± 8.0 (p < 0.001), the Boon walking score improved from 8.9 ± 3.5-7.8 ± 4.4 (p = 0.008), the Boon step score improved from 6.3 ± 2.3-5.2 ± 2.1 (p < 0.001), the Boon time score improved from 4.9 ± 2.4-3.7 ± 2.3 (p < 0.001), tandem walking disturbance improved from 1.7 ± 0.7-1.4 ± 0.9 (p = 0.043), tendency toward falling improved from 1.7 ± 0.7-1.3 ± 1(p = 0.022), 3-meter timed up and go test (3-mTUG) improved from 21.9 ± 7.1-17.6 ± 5.1( p < 0.001), 10-meter walking (10-MWT) step improved from 31.1 ± 13.1-24.6 ± 7.5 (p < 0.001), velocity improved from 0.7 ± 0.2-0.8 ± 0.3 (p < 0.001) and stride length improved from 0.4 ± 0.1-0.46 ± 0.1(p < 0.001), compared with before the CSF TT.

Conclusion: These results suggest that many parameters in the Boon gait test were responsive to the CSF TT, and the Boon gait test may help objectify response to the CSF TT by combining the 10-MWT and 3-mTUG gait assessments.
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http://dx.doi.org/10.1016/j.clineuro.2021.106952DOI Listing
September 2021

Relieving Cellular Energy Stress in Aging, Neurodegenerative, and Metabolic Diseases, SIRT1 as a Therapeutic and Promising Node.

Front Aging Neurosci 2021 20;13:738686. Epub 2021 Sep 20.

Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

The intracellular energy state will alter under the influence of physiological or pathological stimuli. In response to this change, cells usually mobilize various molecules and their mechanisms to promote the stability of the intracellular energy status. Mitochondria are the main source of ATP. Previous studies have found that the function of mitochondria is impaired in aging, neurodegenerative diseases, and metabolic diseases, and the damaged mitochondria bring lower ATP production, which further worsens the progression of the disease. Silent information regulator-1 (SIRT1) is a multipotent molecule that participates in the regulation of important biological processes in cells, including cellular metabolism, cell senescence, and inflammation. In this review, we mainly discuss that promoting the expression and activity of SIRT1 contributes to alleviating the energy stress produced by physiological and pathological conditions. The review also discusses the mechanism of precise regulation of SIRT1 expression and activity in various dimensions. Finally, according to the characteristics of this mechanism in promoting the recovery of mitochondrial function, the relationship between current pharmacological preparations and aging, neurodegenerative diseases, metabolic diseases, and other diseases was analyzed.
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http://dx.doi.org/10.3389/fnagi.2021.738686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489683PMC
September 2021

Reprogrammable optical metasurfaces by electromechanical reconfiguration.

Opt Express 2021 Sep;29(19):30751-30760

Metasurfaces, with artificially designed ultrathin and compact optical elements, enable versatile manipulation of the amplitude, phase, and polarization of light waves. While most of the metasurfaces are static and passive, here we propose a reprogrammable metasurface based on the state-of-art electromechanical nano-kirigami, which allows for independent manipulation of pixels at visible wavelengths through mechanical deformation of the nanostructures. By incorporating electrostatic forces between the top suspended gold nano-architectures and bottom silicon substrate, out-of-plane deformation of each pixel and the associated phase retardation are independently controlled by applying single voltage to variable pixels or exerting programmable voltage distribution on identical pixels. As a proof-of-concept demonstration, the metasurfaces are digitally controlled and a series of tunable metasurface holograms such as 3D dynamic display and ultrathin planar lenses are achieved at visible wavelengths. The proposed electromechanical metasurface provides a new methodology to explore versatile reconfigurable and programmable functionalities that may lead to advances in a variety of applications such as hologram, 3D displays, data storage, spatial light modulations, and information processing.
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http://dx.doi.org/10.1364/OE.434321DOI Listing
September 2021

Mitophagy in the basolateral amygdala mediates increased anxiety induced by aversive social experience.

Neuron 2021 Sep 27. Epub 2021 Sep 27.

Section on Synapse Development Plasticity, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address:

Psychosocial stress is a common risk factor for anxiety disorders. The cellular mechanism for the anxiogenic effect of psychosocial stress is largely unclear. Here, we show that chronic social defeat (CSD) stress in mice causes mitochondrial impairment, which triggers the PINK1-Parkin mitophagy pathway selectively in the amygdala. This mitophagy elevation causes excessive mitochondrial elimination and consequent mitochondrial deficiency. Mitochondrial deficiency in the basolateral amygdalae (BLA) causes weakening of synaptic transmission in the BLA-BNST (bed nucleus of the stria terminalis) anxiolytic pathway and increased anxiety. The CSD-induced increase in anxiety-like behaviors is abolished in Pink1 and Park2 mice and alleviated by optogenetic activation of the BLA-BNST synapse. This study identifies an unsuspected role of mitophagy in psychogenetic-stress-induced anxiety elevation and reveals that mitochondrial deficiency is sufficient to increase anxiety and underlies the psychosocial-stress-induced anxiety increase. Mitochondria and mitophagy, therefore, can be potentially targeted to ameliorate anxiety.
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http://dx.doi.org/10.1016/j.neuron.2021.09.008DOI Listing
September 2021

Treatment of nevus of Ota with 1064 nm picosecond Nd:YAG laser: A retrospective study.

Dermatol Ther 2021 Oct 5:e15152. Epub 2021 Oct 5.

Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu, China.

Nevus of Ota has been successfully treated by lasers. Currently, 1064 nm picosecond Nd:YAG lasers have become available for the treatment of pigmented disorders. However, there are few studies concerning the application of 1064 nm picosecond Nd:YAG laser in nevus of Ota. This study aimed to evaluate the efficacy and safety of a 1064 nm picosecond Nd:YAG laser for the treatment of nevus of Ota. We conducted a retrospective analysis of Chinese patients with nevus of Ota who had been treated with a 1064 nm picosecond Nd:YAG laser. Those who had any other laser treatment during the period of picosecond laser treatment were excluded. Via a visual analog scale for percentage of pigmentary clearance in standard photographs, the treatment efficacy was assessed by three blinded physician evaluators. A total of 16 subjects were included in this retrospective study. The average age at the beginning of treatment was 16.87 years old (range of 4 months to 59 years), and all patients were of Fitzpatrick skin type IV. Total treatment ranged from 1 to 5 sessions. A 1064 nm picosecond Nd:YAG laser with a mean fluence of 1.8-4.3 J/cm was used at 3-12 month intervals. The mean efficacy score for all 16 patients was 2.56 after one session, and the mean efficacy score of 13 patients who completed two sessions and nine patients who completed three sessions were 3.15 and 3.51, respectively. Postinflammatory hyperpigmentation after treatment was only observed in 1 (1/16, 6.25%) patient. The 1064 nm picosecond Nd:YAG laser is an effective and safe approach for treating nevus of Ota.
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http://dx.doi.org/10.1111/dth.15152DOI Listing
October 2021

Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis.

Nat Chem Biol 2021 Oct 4. Epub 2021 Oct 4.

MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.

Spindle position control is essential for cell fate determination and organogenesis. Early studies indicate the essential role of the evolutionarily conserved Gαi/LGN/NuMA network in spindle positioning. However, the regulatory mechanisms that couple astral microtubules dynamics to the spindle orientation remain elusive. Here we delineated a new mitosis-specific crotonylation-regulated astral microtubule-EB1-NuMA interaction in mitosis. EB1 is a substrate of TIP60, and TIP60-dependent crotonylation of EB1 tunes accurate spindle positioning in mitosis. Mechanistically, TIP60 crotonylation of EB1 at Lys66 forms a dynamic link between accurate attachment of astral microtubules to the lateral cell cortex defined by NuMA-LGN and fine tune of spindle positioning. Real-time imaging of chromosome movements in HeLa cells expressing genetically encoded crotonylated EB1 revealed the importance of crotonylation dynamics for accurate control of spindle orientation during metaphase-anaphase transition. These findings delineate a general signaling cascade that integrates protein crotonylation with accurate spindle positioning for chromosome stability in mitosis.
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http://dx.doi.org/10.1038/s41589-021-00875-7DOI Listing
October 2021

The rhizosphere effect of native legume Albizzia julibrissin on coastal saline soil nutrient availability, microbial modulation, and aggregate formation.

Sci Total Environ 2021 Sep 30:150705. Epub 2021 Sep 30.

Institute of Subtropical Crops of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Wenzhou 325005, China. Electronic address:

In the coastal zones, numerous ecological shelterbelt projects were conducted to protect against natural hazards. However, it is still not fully understood whether phytoremediation with native legume Albizzia julibrissin plantation can improve saline soil structural development or microbial community structure. In this study, a field experiment was conducted to investigate the responses of rhizosphere soil salinity, nutrients, bacterial community, and aggregate structure to A. julibrissin plantation in a recently reclaimed area along Zhejiang coast, China. After ~3-year plantation, rhizosphere soil pH and EC reduced to 8.25 and 0.14 dS·m, respectively, belonging to non-saline soil. Meanwhile, total organic carbon (TOC), permanganate-oxidizable carbon (POXC), total nitrogen (TN), alkali-hydrolyzable nitrogen (AN), and ammonium nitrogen (NH-N) were significantly increased in rhizosphere soil compared with bare land (P < 0.05). Consequently, rhizosphere soil had favorable habitat condition for copiotrophic bacterial taxa (e.g., Chloroflexi, Acidobacteria, and Bacteroidates), as well as high diversity, complex co-occurrence network, and catabolism related with nutrient cycling. The soil particle size of bare land was <0.053 mm, while microaggregate (0.053-0.25 mm) and macroaggregate (0.25-2 mm) were formed in the rhizosphere and coupled with C accumulation and Fe removal. Soil aggregates were of great importance to soil fertility with more efficient bacterial network and biogeochemical cycles of nutrients. N-fixing Rhizobiales preferred to inhabit large soil particle and might primarily contribute to N accumulation. Generally, A. julibrissin was a suitable pioneer tree for mudflat reclamation projects, which effectively improved saline soil rhizosphere environment by reducing salinity, accumulating C and N, and promoting microbial community succession, as well as aggregate structure formation.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150705DOI Listing
September 2021

Efficacy Analysis of Day Surgery A1 Pulley Release for Pediatric Trigger Thumb.

Front Pediatr 2021 14;9:734115. Epub 2021 Sep 14.

Department of Orthopedics, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, National Clinical Research Center for Child Health and Disorders, Chongqing, China.

To investigate clinical application of day surgery A1 pulley release for pediatric trigger thumb. We retrospectively analyzed the clinical data of 1,642 children with trigger thumb who were treated with day surgery A1 pulley release at our hospital, including satisfaction surveys, functional recovery, and complications. The operative time for unilateral and bilateral tenolysis was 4.8 ± 3.1 and 9.2 ± 3.8 min, respectively. Three children had postoperative fever and were discharged on the 2nd day after surgery. The rest of the children were discharged on the day of surgery. All incisions healed primarily, and no complications of vascular and nerve injury were reported. The patients' degree of satisfaction with the medical treatment process, diagnosis and treatment workflow, treatment effectiveness, length of hospital stay and hospitalization cost, and discharge guidance were 97.9, 96.1, 99.3, 91.1, and 98.5%, respectively. The follow-up period was between 5 months and 3 years and 1 month. Four children experienced symptom relapse after the operation, and re-tenolysis was performed in one of them. At the final follow-up, the appearance and function of the thumb had recovered well in all cases. Day surgery A1 pulley release can effectively release tendon sheaths and has a short operative time, no complications of vascular and nerve injury, and good recovery of thumb function. It is a safe and reliable procedure with high patient satisfaction, and it is worthy of clinical promotion.
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http://dx.doi.org/10.3389/fped.2021.734115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477024PMC
September 2021

Several anthropometric measurements and cancer mortality: predictor screening, threshold determination, and joint analysis in a multicenter cohort of 12138 adults.

Eur J Clin Nutr 2021 Sep 28. Epub 2021 Sep 28.

Department of Gastrointestinal Surgery and Department of Clinical Nutrition, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.

Background: Anthropometric measurements (AMs) are cost-effective surrogates for evaluating body size. This study aimed to identify the optimal prognostic AMs, their thresholds, and their joint associations with cancer mortality.

Methods: We performed an observational cohort study including 12138 patients with cancer at five institutions in China. Information on demographics, disease, nutritional status, and AMs, including the body mass index, mid-arm muscle circumference, mid-arm circumference, handgrip strength, calf circumference (CC), and triceps-skinfold thickness (TSF), was collected and screened as mortality predictors. The optimal stratification was used to determine the thresholds to categorize those prognostic AMs, and their associations with mortality were estimated independently and jointly by calculating multivariable-adjusted hazard ratios (HRs).

Results: The study included 5744 females and 6394 males with a mean age of 56.9 years. The CC and TSF were identified as better mortality predictors than other AMs. The optimal thresholds were women 30 cm and men 32.8 cm for the CC, and women 21.8 mm and men 13.6 mm for the TSF. Patients in the low CC or low TSF group had a 13% (HR = 1.13, 95% CI = 1.03-1.23) and 22% (HR = 1.22, 95% CI = 1.12-1.32) greater mortality risk compared with their normal CC/TSF counterparties, respectively. Concurrent low CC and low TSF showed potential joint effect on mortality risk (HR = 1.39, 95% CI = 1.25-1.55).

Conclusions: These findings support the importance of assessing the CC and TSF simultaneously in hospitalized cancer patients to guide interventions to optimize their long-term outcomes.
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http://dx.doi.org/10.1038/s41430-021-01009-xDOI Listing
September 2021

Cross-cultural validation of the IRB Researcher Assessment Tool: Chinese Version.

BMC Med Ethics 2021 Sep 28;22(1):133. Epub 2021 Sep 28.

Center for Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, People's Republic of China.

Background: Using an effective method for evaluating Institutional Review Board (IRB) performance is essential for ensuring an IRB's effectiveness, efficiency, and compliance with applicable human research standards and organizational policies. Currently, no empirical research has yet been published in China evaluating IRB performance measures by the use of a standardized tool. This study was therefore conducted to develop a Chinese version of the IRB Researcher Assessment Tool (IRB-RAT), assess the psychometric properties of the Chinese version (IRB-RAT-CV), and validate the tool for use in China.

Methods: In this cultural adaptation, cross-sectional validation study, the IRB-RAT-CV was developed through a back-translation process and then distributed to 587 IRB staff members and researchers in medical institutions and schools in Hunan Province that review biomedical and social-behavioral research. Data from the 470 valid questionnaires collected from participants was used to evaluate the reliability, content validity, and construct validity of the IRB-RAT-CV.

Results: Participants' ratings of their ideal and actual IRB as measured by the IRB-RAT-CV achieved Cronbach's alpha 0.989 and 0.992, Spearman-Brown coefficient 0.964 and 0.968, and item-total correlation values ranging from 0.631 to 0.886 and 0.743 to 0.910, respectively.

Conclusion: The IRB-RAT-CV is a linguistically and culturally applicable tool for assessing the quality of IRBs in China.
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http://dx.doi.org/10.1186/s12910-021-00699-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479956PMC
September 2021

Analysis of Gene Family and Its Function on Cell Lignification in Pears ().

Plants (Basel) 2021 Sep 10;10(9). Epub 2021 Sep 10.

Pear Engineering Research Centre, College of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.

Class III peroxidases (PRXs) are plant-specific enzymes that play key roles in the responses to biotic and abiotic stress during plant growth and development. In addition, some peroxidases also play roles in plant lignification. In this study, a total of 114 (designated ) genes were identified in the pear () genome based on systematic analysis. These genes were divided into 12 groups based on their phylogenetic relationships. We performed systematic bioinformatics analysis of the genes, including analysis of gene structures, conserved motifs, phylogenetic relationships, and gene expression patterns during pear fruit growth. The are unevenly distributed on the 17 pear chromosomes and some of them on other scaffolds. Gene duplication event analysis indicated that whole-genome duplication (WGD) and segmental duplication play key roles in gene amplification. Ka/Ks analysis suggested that most duplicated experienced purifying selection, with limited functional divergence during the duplication events. Furthermore, the analysis indicated that those highly expressed genes might play significant roles in the lignification of cells to form stone cells in pear fruit. We examined the expression of those highly expressed genes during fruit growth using quantitative real-time PCR (qRT-PCR), verifying differential expression patterns at different stages of fruit. This study provides useful information for further functional analysis of the gene family in pears.
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http://dx.doi.org/10.3390/plants10091874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470002PMC
September 2021

Cellular Organelles Involved in Hepatitis E Virus Infection.

Pathogens 2021 Sep 17;10(9). Epub 2021 Sep 17.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.

Hepatitis E virus (HEV), a major cause of acute hepatitis worldwide, infects approximately 20 million individuals annually. HEV can infect a wide range of mammalian and avian species, and cause frequent zoonotic spillover, increasingly raising public health concerns. To establish a successful infection, HEV needs to usurp host machineries to accomplish its life cycle from initial attachment to egress. However, relatively little is known about the HEV life cycle, especially the functional role(s) of cellular organelles and their associated proteins at different stages of HEV infection. Here, we summarize current knowledge regarding the relation of HEV with the different cell organelles during HEV infection. Furthermore, we discuss the underlying mechanisms by which HEV infection is precisely regulated in infected cells and the modification of host cell organelles and their associated proteins upon HEV infection.
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http://dx.doi.org/10.3390/pathogens10091206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469867PMC
September 2021

Complete chloroplast genome sequence of (Saxifragaceae).

Mitochondrial DNA B Resour 2021 22;6(10):3028-3030. Epub 2021 Sep 22.

Laboratory of Extreme Environmental Biological Resources and Adaptive Evolution, College of Science, Tibet University, Lhasa, China.

Bunge, Tibetan name 'Yajima,' growing in the highlands of China is a perennial herb belonging to the genus Saxifragaceae. As a traditional Chinese medicine, it has been used to treat digestive diseases for hundreds of years. The complete chloroplast genome of is 152,775 bp in length and comprises two inverted repeats (IR, 25,962 bp), a large single-copy region (LSC, 83,533 bp), and a small single-copy region (SSC, 17,318 bp). It harbors 112 genes, including 78 protein-coding genes, four ribosomal RNA genes, and 30 transfer RNA genes. In addition, the gene was deleted. The GC content of the whole chloroplast genome is 37.54%. This chloroplast genome resource will be useful for study on the evolution and genetic diversity of in the future.
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http://dx.doi.org/10.1080/23802359.2021.1978893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8462875PMC
September 2021
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