Publications by authors named "Xing Liang"

185 Publications

Hyperglycemia accelerates inflammaging in the gingival epithelium through inflammasomes activation.

J Periodontal Res 2021 Mar 2. Epub 2021 Mar 2.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Background And Objective: Diabetes accelerates inflammaging in various tissue with an increase in senescent cell burden and senescence-associated secretory phenotype (SASP) secretion, which is a significant cause of tissue dysfunction and contributes to the diabetic complications. Recently, inflammasomes are thought to contribute to inflammaging. Here, utilizing diabetic models in vivo and in vitro, we investigated the potential association between hyperglycemia-induced inflammaging and gingival tissue dysfunction and the mechanism underlying inflammasome-associated inflammaging.

Materials And Methods: Gingival epithelium and serum were collected from control and diabetic patients and mice. The expression of p16, p21, and inflammasomes in the gingival epithelium, SASP factors in serum, and the molecular factors associated with gingival epithelial barrier function were assessed. Human oral keratinocyte (HOK) was stimulated with normal and high glucose, and pre-treated with Z-YVAD-FMK (Caspase-1 inhibitor) prior to evaluating cellular senescence, SASP secretion, and inflammasome activation.

Results: In vivo, hyperglycemia significantly elevated the local burden of senescent cells in the gingival epithelium and SASP factors in the serum and simultaneously reduced the expression levels of Claudin-1, E-cadherin, and Connexin 43 in the gingival epithelium. Interestingly, the inflammasomes were activated in the gingival epithelium. In vitro, high glucose-induced the inflammaging in HOK, and blocking inflammasome activation through inhibiting Caspase-1 and glucose-induced inflammaging.

Conclusions: Hyperglycemia accelerated inflammaging in the gingival epithelium through inflammasomes activation, which is potentially affiliated with a decline in the gingival epithelial barrier function in diabetes. Inflammasomes-related inflammaging may be the crucial mechanism underlying diabetic periodontitis and represents significant opportunities for advancing prevention and treatment options.
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http://dx.doi.org/10.1111/jre.12863DOI Listing
March 2021

Ammonium and nitrate sources and transformation mechanism in the Quaternary sediments of Jianghan Plain, China.

Sci Total Environ 2021 Feb 9;774:145131. Epub 2021 Feb 9.

School of Environmental Studies, China University of Geosciences, Wuhan 430078, China.

Excessive inorganic nitrogen (IN) compound content in groundwater is generally attributed to anthropogenic activities. Here, natural nitrogen sources in Quaternary sediments from aquifers and aquitards of Jianghan Plain (JHP), China were identified. Ammonium and nitrate content in groundwater samples collected from 129 well sites were determined through chemical analysis. Subsequent 4 boreholes were drilled at areas with high nitrogen concentration in the Quaternary aquifer. Indicators from hydrochemistry and soil geochemistry analysis, as well as optically stimulated luminescence dating and various of radioactive isotope δC-CO and stable isotopes including δN-NH, δN-total organic nitrogen (TON), δN-NO, δO-NO, δO-HO, δD-HO, and δC-total organic carbon (TOC) were used to identify high-concentration N compound sources and transformation mechanisms (NO: 0.02-770 mg L; NH-N: 0-30.5 mg L) in the porous media. The thick clay layer protected the underlying media. Paleo-precipitation characteristics were preserved in the porewater; that is, it had not been affected by anthropogenic activities. The high nitrate concentration in the shallow oxidized aquifer was mostly attributed to manure and sewage (δN-NO was 14‰). The ammonium-N in the deep strata and part of ammonium-N in the shallow strata (aquifers and aquitards) were from natural sources, mainly from natural TON mineralization. Adsorption was an auxiliary factor for ammonium enrichment in the shallow strata, as were dissimilatory nitrate reduction to ammonium (DNRA) and low ammonia volatilization. Organic matter (OM) involved in mineralization was a mixture of lacustrine algae and terrigenous clastic sediments (from river upstream). The algae were traced to lake formation and frequent evolutionary changes in river environments, as indicated by alterations in sedimentary facies. The present findings may encourage researchers to consider natural IN sources' contribution to N contamination using quantitative models. They also serve as a valuable reference for understanding other pollutants' transformation mechanism in similar environments and provide research ideas for similar areas.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145131DOI Listing
February 2021

The RNA methyltransferase NSUN6 suppresses pancreatic cancer development by regulating cell proliferation.

EBioMedicine 2021 Jan 6;63:103195. Epub 2021 Jan 6.

The Core Laboratory in Medical Centre of Clinical Research, Department of Endocrinology, Shanghai Ninth People's Hospital, State Key Laboratory of Medical Genomics, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; Department of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. Electronic address:

Background: Pancreatic cancer (PC) is one of the most lethal solid malignancies in the world due to its excessive cell proliferation and aggressive metastatic features. Emerging evidences revealed the importance of posttranscriptional modifications of RNAs in PC progression. However, knowledge about the 5-methylcytosine (m5C) RNA modification in PC is still extremely limited. In this study, we attempted to explore the expression changes and clinical significances of 12 known m5C-related genes among PC patients.

Methods: A total of 362 normal and 382 tumor specimens from PC patients were examined for candidate m5C-related gene and protein expression by using quantitative PCR (qPCR) and immunohistochemistry (IHC). The proliferation rate of PC cells was detected by MTS assay. Xenograft mouse models were used to assess the role of NSUN6 in PC tumor formation.

Findings: Through analyzing the four Gene Expression Omnibus (GEO) databases, six m5C-related genes shown significant and consistent alterations were selected for further examination in our 3 independent PC cohorts. Finally, we identified the reduction of NSUN6 as a common feature of all PC sample sets examined. NSUN6 expression correlated with clinicopathologic parameters including T stage, and Ki67 cell rate. Further assessing the transcriptional profiles of 50 PC tissues, we found biological processes associated with cell proliferation like cell cycle and G2M checkpoint were enriched in NSUN6 lower expression group. Helped by in vitro PC cell lines and in vivo xenograft mouse models, we confirmed the role of NSUN6 in regulating cell proliferation and PC tumor growth. Last but also importantly, we also show the good performance of NSUN6 in evaluating tumor recurrence and survival among PC patients.

Interpretation: Our data suggested that NSUN6 is an important factor involved in regulating cell proliferation of PC, and highlights the potential of novel m5C-based clinical modalities as a therapeutic approach in PC patients.

Funding: This study was supported by the National Natural Science Foundation of China (Grant Nos. 81803014, 81802424, and 81802911).
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http://dx.doi.org/10.1016/j.ebiom.2020.103195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804980PMC
January 2021

A novel monocanalicular silicone intubation technique for canalicular laceration repair.

J Plast Reconstr Aesthet Surg 2020 Dec 14. Epub 2020 Dec 14.

Department of Ophthalmology, Shanxi Dayi Hospital, No. 99 of Longcheng Street, Taiyuan 030012, PR China.

Objectives: This study aimed to evaluate the clinical efficacy and reliability of a novel monocanalicular silicone intubation (nMCI) technique for canalicular laceration repair in a series of patients treated over a 3-year period.

Methods: The case records of 86 patients (86 eyes) who had undergone nMCI-based surgical repair of canalicular lacerations were retrospectively reviewed. The silicone tube removal was planned for 3 months postoperatively. A minimal follow-up of 6 months was adopted for the final analysis. The primary outcome measures included anatomical patency on irrigation and relief from epiphora, which were assessed subjectively and objectively through a fluorescein dye disappearance test.

Results: A total of 86 patients (72 males and 14 females; mean age = 34 years) were included. The upper canaliculus was damaged in 13 cases, while the lower canaliculus was damaged in 73 cases. Successful stenting was achieved in all cases. The postoperative eyelid position was satisfactory in the majority of the patients. The mean period of stenting was 3 months, while the mean postoperative follow-up period was 6 months. There were no cases of premature stent extrusion. The functional drainage following stent removal was normal in 95% of the patients, while the syringing revealed full patency with no narrowing or reflux.

Conclusion: The present results suggest that the nMCI technique presents an effective and atraumatic surgical approach for adult patients with canalicular lacerations. The main advantages of the technique are the simple insertion and the easy removal of the tube, which results in high anatomical and functional success rates and offers an effective alternative in the treatment of monocanalicular lacerations.
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http://dx.doi.org/10.1016/j.bjps.2020.12.021DOI Listing
December 2020

[Current Advances in Peri-implantitis Mouse Model].

Sichuan Da Xue Xue Bao Yi Xue Ban 2020 Nov;51(6):767-770

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Peri-implantitis is the most common and intractable complication of dental implant-supported prothesis affecting its long-term success, and is one of the main reasons for implant failure. Due to the limitation of the research methods, the pathogenesis and pathological processes of peri-implantitis remain unclear. Animal models are indispensable tools to study the pathogenesis of diseases. With the advances of the dental implants, the peri-implantitis mouse model has been used in experimental research. This paper summarized recent studies from the following five aspects: the advantages of the mouse model, the influence of mouse strain, the design of micro-implant, the way of implant insertion, as well as the induction of peri-implantitis, aiming to provide references and help for researchers. Compared with the large animal models of peri-implantitis, the mouse model of peri-implantitis is more flexible in use. Lower costs can better control the sample number and shorter induction time can better control experimental duration. The completion of mouse genome sequencing and the progress of the genetic operating system also make the pathogenetic study possible. However, the mouse model of peri-implantitis still has some limitations. Limited by the small size of mouse oral cavity, implant insertion surgery is technically demanding, and complex surgeries are even more challenging. Moreover, due to short history of the peri-implantitis mouse model, its corresponding technical theories such as implantation methods, peri-implantitis induction methods and so on are not unified yet and still need further research and development.
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http://dx.doi.org/10.12182/20201160205DOI Listing
November 2020

Organic fluorescent probes for monitoring autophagy in living cells.

Chem Soc Rev 2021 Jan 6;50(1):102-119. Epub 2020 Nov 6.

School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Daxue Road 3501, Changqing District, Jinan 250353, P. R. China.

As a ubiquitous degradation process in cells, autophagy plays important roles in various biological activities. However, the abnormality of autophagy is closely related to many diseases, such as aging, neurological disorder, and cancer. Thus, monitoring the process of autophagy in living cells has high significance in biological studies and diagnosis of related diseases. In order to real-time and in situ monitor the process of autophagy, various organic fluorescent probes have been explored in recent years owing to the advantages such as handy staining processes, flexible molecular design strategies, and near-nondestructive detection. However, this interesting and frontier topic has not been reviewed so far. In this tutorial review, we will focus on the latest breakthrough results of organic fluorescent probes in monitoring autophagy of living cells, especially the probe design strategies based on the several microenvironment changes of the autophagy process, and the responding mechanisms and bio-imaging applications in the autophagy process. In addition, we will discuss the shortcomings and limitations of the probes developed, such as susceptible to interference, unable to monitor the whole process, and lack of clinical applications. Finally, we will highlight some challenges and further opportunities in this field. This tutorial review may promote the development of more robust fluorescent probes to further reveal the mechanisms of autophagy, which is the basis of degradation and recycling of cell components.
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http://dx.doi.org/10.1039/d0cs00896fDOI Listing
January 2021

[Mechanism of Inorganic Nitrogen Transformation and Identification of Nitrogen Sources in Water and Soil].

Huan Jing Ke Xue 2020 Sep;41(9):4333-4344

School of Environmental Studies, China University of Geosciences, Wuhan 430074, China.

Nitrogen plays a vital role in biological activities as the basic element of organic molecules and the main nutrient of soil. In the study of nitrogen pollution, the first step is to understand the transformation mechanism of various nitrogen forms. Based on the transformation process and the mechanism of nitrogen in the ecosystem, this review summarizes the research methods of nitrogen source analysis and nitrogen isotope fractionation in soil and water, and categorically reviews the applications of nitrogen source identification in surface water and groundwater. We showed that it is more effective to identify the sources of soil and water nitrogen pollution by combining hydrochemical methods with the multi-isotope approach. The importance of primary nitrogen sources should also be quantified to study groundwater pollution. A new approach to determine the source of oxygen atoms during nitrosation was also presented, and the enrichment principle of O-NO during secondary oxidation was explained. Finally, the contribution of primary ammonium nitrogen to groundwater pollution was discussed, and innovative research ideas were provided.
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http://dx.doi.org/10.13227/j.hjkx.202001047DOI Listing
September 2020

Investigation of esthetic evaluation and its influencing factors for a tunnel portal based on dynamic vision.

PLoS One 2020 23;15(9):e0238762. Epub 2020 Sep 23.

School of Highway, Chang'an University, Xi'an, Shanxi, China.

With the development of modern cities, roads, and landscapes, it is becoming increasingly important for infrastructure such as tunnels to provide an esthetically pleasing experience. In this respect, it is necessary to conduct studies that consider the esthetic design of tunnel portals using esthetics research. Regarding the esthetic evaluation of tunnel portals, this paper fully considers the dynamic visual effect from the driver's perspective. This study combines the use of Blender, SpeedTree Modeler Cinema, Adobe Photoshop CS6, and other software for secondary development. These programs are connected to the driving simulation platform Euro Truck Simulator 2 (which is equipped with a driving simulator) to construct a set of driving simulation tests that enable the esthetic evaluation of a tunnel portal. The Banlun Tunnel on the Funing-Longliu Expressway in Yunnan Province, China, is used as a case study, and four impact factors that vary significantly in esthetic design are included: the linearity, color, greening and texture of the portal. Using an orthogonal experimental design, the influence of the esthetic degree was simulated and evaluated, and the order of sensitivity to esthetic factors of a headwall tunnel portal was sequentially determined as follows: the portal texture exerts the maximum impact on the beauty degree of the headwall portal, followed by the portal greening and the portal color, while the portal linearity exerts the minimum impact. The results show that the developed driving simulation test system can be used to determine the sensitivity of esthetic factors for a tunnel portal and obtain an optimal collocation of esthetic factors on different levels; hence, it provides feedback for use in designing the optimum esthetic tunnel portal. This test system can be used as a reference when conducting future evaluations and studies on tunnel portal esthetics.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238762PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510998PMC
November 2020

Synthesis, Characterization, Photoluminescence, Molecular Docking and Bioactivity of Zinc (II) Compounds Based on Different Substituents.

Molecules 2020 Jul 29;25(15). Epub 2020 Jul 29.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China.

Six new zinc(II) complexes were prepared by the reaction of ZnBr or ZnI with 4'-(substituted-phenyl)-2,2':6',2''-terpyridine compounds, bearing -methylsulfonyl (L), -methoxy (L) and -methyl (L), which were characterized by elemental analysis, FT-IR, NMR and single crystal X-ray diffraction. The antiproliferative properties against Eca-109, A549 and Bel-7402 cell lines and the cytotoxicity test on RAW-264.7 of these compounds were monitored using a CCK-8 assay, and the studies indicate that the complexes show higher antiproliferative activities than cisplatin. The interactions of these complexes with CT-DNA and proteins (BSA) were studied by UV-Vis, circular dichroism (CD) and fluorescent spectroscopy, respectively. The results indicate that the interaction of these zinc(II) complexes with CT-DNA is achieved through intercalative binding, and their strong binding affinity to BSA is fulfilled through a static quenching mechanism. The simulation of the complexes with the CT-DNA fragment and BSA was studied by using molecular docking software. It further validates that the complexes interact with DNA through intercalative binding mode and that they have a strong interaction with BSA.
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http://dx.doi.org/10.3390/molecules25153459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436059PMC
July 2020

Clinical Efficacy of Chlorhexidine as an Adjunct to Mechanical Therapy of Peri-Implant Disease: A Systematic Review and Meta-Analysis.

J Oral Implantol 2021 Feb;47(1):78-87

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

The aim of this systematic review and meta-analysis was to determine the efficacy of chlorhexidine (CHX) as an adjunctive therapy to mechanical debridement in the treatment of peri-implant diseases. Five databases (PubMed, EMBASE, Cochrane Central Register of Controlled Trails, Web of Science, and ClinicalTrials.gov) were searched. Randomized controlled trials (RCTs) comparing mechanical debridement combined with CHX to mechanical debridement alone for patients with peri-implant disease were identified. The trial investigators evaluated factors indicating inflammatory levels, including bleeding on probing (BOP), probing depth (PD), and clinical attachment level (CAL). Six RCTs with 207 patients were ultimately included in this review. Low- to moderate-quality evidence demonstrated that the adjunctive CHX therapy had no significant effect on BOP reduction within 1 month (mean difference [MD], 0.10; 95% confidence interval [CI], -0.06 to 0.25), 3-4 months (MD, 0.06; 95% CI, -0.03 to 0.15), and 6-8 months (MD, 0.06; 95% CI, -0.03 to 0.14) of follow-up. Significant differences in PD reduction and CAL gain were also not found. Although 1 subgroup analysis revealed a significant result (MD, 009; 95% CI, 0.01-0.18) for the use of CHX solution, this could be interpreted as clinically slight. Based on available evidence, adding CHX to mechanical debridement, compared with mechanical debridement alone, did not significantly enhance the clinical results. Therefore, clinicians should consider the negligible effect of adjunctive CHX.
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http://dx.doi.org/10.1563/aaid-joi-D-19-00213DOI Listing
February 2021

Growth cone-localized microtubule organizing center establishes microtubule orientation in dendrites.

Elife 2020 07 13;9. Epub 2020 Jul 13.

Department of Biology, Stanford University, Stanford, United States.

A polarized arrangement of neuronal microtubule arrays is the foundation of membrane trafficking and subcellular compartmentalization. Conserved among both invertebrates and vertebrates, axons contain exclusively 'plus-end-out' microtubules while dendrites contain a high percentage of 'minus-end-out' microtubules, the origins of which have been a mystery. Here we show that in the dendritic growth cone contains a non-centrosomal microtubule organizing center (MTOC), which generates minus-end-out microtubules along outgrowing dendrites and plus-end-out microtubules in the growth cone. RAB-11-positive endosomes accumulate in this region and co-migrate with the microtubule nucleation complex γ-TuRC. The MTOC tracks the extending growth cone by kinesin-1/UNC-116-mediated endosome movements on distal plus-end-out microtubules and dynein clusters this advancing MTOC. Critically, perturbation of the function or localization of the MTOC causes reversed microtubule polarity in dendrites. These findings unveil the endosome-localized dendritic MTOC as a critical organelle for establishing axon-dendrite polarity.
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http://dx.doi.org/10.7554/eLife.56547DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7375809PMC
July 2020

Synthesis, characterization, photoluminescence, antiproliferative activity, and DNA interaction of cadmium(II) substituted 4'-phenyl-terpyridine compounds.

J Inorg Biochem 2020 09 25;210:111165. Epub 2020 Jun 25.

School of Chemistry and Chemical Engineering, Guangxi University, 530004 Nanning, Guangxi, People's Republic of China; Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. Electronic address:

A series of CdCl complexes (1a-1f and 2a-2c) with 4'-(substituted-phenyl)-2,2':6',2″-terpyridine compounds bearing hydrogen (L), p-methyl (L), p-phenyl (L), p-tolyl (L), p-carboxyl (L), p-fluoro (L), p-hydroxyl (L), m-hydroxyl (L) or o-hydroxyl (L), were prepared and characterized by H NMR, IR, elemental analysis and single crystal X-ray diffraction. All the compounds display interesting photoluminescent properties and different maximal emission peaks due to the difference of the substituent groups. The in vitro antiproliferative activities against four human carcinoma cell lines, A549, Bel-7402, Eca-109 and MCF-7, were investigated and cell viability studies indicate that the compounds have excellent results with the lowest IC values of 0.372 (1c), 1.003 (1c), 1.161 (1b) and 0.231 (1c) μM, respectively. The DNA interaction was studied by fluorescence titration, circular dichroism spectroscopy and molecular modeling methods. Spectrophotometric results reveal that the compounds have strong affinity binding with DNA as intercalators and molecular docking studies indicate that the binding is contributed by the π…π stacking and hydrogen bonds.
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http://dx.doi.org/10.1016/j.jinorgbio.2020.111165DOI Listing
September 2020

Preparation and properties of amphiphilic hydrophobically associative polymer/ montmorillonite nanocomposites.

R Soc Open Sci 2020 May 20;7(5):200199. Epub 2020 May 20.

CNPC Nanochemistry Key Laboratory, College of Science, China University of Petroleum, Beijing 102249, People's Republic of China.

In this research, a novel amphiphilic hydrophobically associative polymer nanocomposite (ADOS/OMMT) was prepared using acrylamide (AM), sodium 4-vinylbenzenesulfonate (SSS), N, N'-dimethyl octadeyl allyl ammonium bromide (DOAAB) and organo-modified montmorillonite (OMMT) through polymerization. Both X-ray diffraction patterns and transmission electron microscopy images verified the dispersion morphology of OMMT in the copolymer matrix. Then, the effect of the introduction of OMMT layers on the copolymer properties was studied by comparing with pure copolymer AM/SSS/DOAAB (ADOS). The thermal degradation results demonstrated that the thermal stability of the ADOS/OMMT were better than pure copolymer ADOS. During the solution properties tests, ADOS/OMMT nanocomposite was superior to ADOS in viscosifying ability, temperature resistance, salt tolerance, shear resistance and viscoelasticity, which was because OMMT contributed to enhance the hydrophobic association structure formed between polymer molecules. Additionally, the ADOS/OMMT nanocomposite exhibited more excellent interfacial activity and crude oil emulsifiability in comparison to pure copolymer ADOS. These performances indicated ADOS/OMMT nanocomposite had good application prospects in tertiary recovery.
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http://dx.doi.org/10.1098/rsos.200199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277241PMC
May 2020

Loganin attenuates intestinal injury in severely burned rats by regulating the toll-like receptor 4/NF-κB signaling pathway.

Exp Ther Med 2020 Jul 7;20(1):591-598. Epub 2020 May 7.

Department of Burns and Plastic Surgery, Affiliated Hospital of Chengde Medical University, Chengde, Hebei 067000, P.R. China.

Severe burns may lead to intestinal inflammation and oxidative stress, resulting in intestinal barrier damage and gut dysfunction. Loganin, an iridoid glycoside compound, has been isolated from Sieb. et Zucc; however, its role in the treatment of burn injury is yet to be fully elucidated. Therefore, the present study examined the effect of loganin administration on burn-induced intestinal inflammation and oxidative stress after severe burns in male Sprague-Dawley rats. Histological injury was assessed by hematoxylin and eosin staining. Furthermore, cytokine expression in intestinal tissues was measured by ELISA and reverse transcription-quantitative PCR. Antioxidative activities were assessed by determining the levels of reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). Apoptosis was detected by flow cytometry. Apoptosis-related proteins, toll-like receptor 4 (TLR4) protein and NF-κB translocation were examined by western blotting. Immunohistochemical staining was used to observe TLR4 and NF-κB p65 expression in intestinal tissues. The present study suggested that loganin administration significantly reduced burn injury-induced intestinal histological changes, tumor necrosis factor-α, interleukin (IL)-6 and IL-1β production and oxidative stress, evidenced by decreased ROS levels and MDA content (P<0.05). Furthermore, loganin increased SOD, CAT and GSH-Px levels and intestinal epithelial cell apoptosis. Loganin treatment also significantly inhibited activation of the TLR4/NF-κB signaling pathway in the intestine of severely burned rats (P<0.05). In conclusion, loganin reduced burns-induced intestinal inflammation and oxidative stress, potentially by regulating the TLR4/NF-κB signaling pathway.
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http://dx.doi.org/10.3892/etm.2020.8725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281942PMC
July 2020

Author Correction: Chronic Kidney Disease Impairs Bone Defect Healing in Rats.

Sci Rep 2020 Jun 8;10(1):9482. Epub 2020 Jun 8.

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41598-020-65651-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280222PMC
June 2020

Effects of Herbal Mouthwashes on Plaque and Inflammation Control for Patients with Gingivitis: A Systematic Review and Meta-Analysis of Randomised Controlled Trials.

Evid Based Complement Alternat Med 2020 20;2020:2829854. Epub 2020 Jan 20.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, South Renmin Road, Chengdu 610041, China.

Objective: The aim of this study was to evaluate the overall effects of herbal mouthwashes as supplements to daily oral hygiene on plaque and inflammation control compared with placebos and chlorhexidine (CHX) mouthwashes in the treatment of gingivitis.

Methods: PubMed, EMBASE, Cochrane Database of Systematic Reviews, Cochrane Central Register of Controlled Trials, and grey literature databases were searched. Only randomised controlled trials (RCTs) comparing herbal mouthwashes with placebos or CHX in the daily oral hygiene of patient with gingivitis were included to compare the effect of different mouthwashes on plaque and inflammation control.

Results: A total of 13 studies satisfied the eligibility criteria, and 11 studies were included in meta-analyses. Significant differences were observed in favour of herbal mouthwashes compared with placebos in both plaque- and inflammation-related indices (Quigley-Hein Plaque Index, QHPI: WMD = -0.61, 95% CI (-0.80, -0.42), < 0.001; Gingival Index, GI: -0.28 (-0.51, -0.06), =0.01; Modified Gingival Index, MGI: -0.59 (-1.08, -0.11), =0.02; Gingival Bleeding Index, GBI: -0.06 (-0.09, -0.04), < 0.001). No significant difference was found between herbal and CHX mouthwashes.

Conclusions: Herbal mouthwashes have potential benefits in plaque and inflammation control as supplements to the daily oral hygiene of patients with gingivitis. Although no difference was observed between herbal and CHX mouthwashes in the selected studies, further high-quality RCTs are needed for more firm support before advising patients with gingivitis about whether they can use herbal mouthwashes to substitute for CHX mouthwashes or not (: CRD42019122841).
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http://dx.doi.org/10.1155/2020/2829854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201456PMC
January 2020

Efficacy of aromatherapy on dental anxiety: A systematic review of randomised and quasi-randomised controlled trials.

Oral Dis 2020 Apr 8. Epub 2020 Apr 8.

State Key Laboratory of Oral Diseases, Department of Prosthodontics, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Objectives: To evaluate the efficacy of aromatherapy on dental anxiety.

Methods: A detailed protocol was registered a priori (PROSPERO: CRD42019141093). PubMed, EMBASE, CDSR, CENTRAL, CNKI and grey literature databases were searched from inception to 10 January 2020. Randomised controlled trials (RCTs) and quasi-randomised controlled trials (CCTs) that examined the effects of aromatherapy on dental anxiety, compare to either negative control (i.e. no aromatherapy) or other positive control approaches (e.g. music), were included. The risk of bias of the selected studies was evaluated using the Cochrane Collaboration's tool and ROBINS-I, while the certainty of evidence was assessed using GRADE. A narrative synthesis was performed, rather than meta-analysis, due to the high level of heterogeneity across studies.

Results: Eleven RCTs and six CCTs were included. The risk of bias was identified as either high or unclear in RCTs, and moderate to serious in CCTs. Performance bias and detection bias were the most prevalent in RCTs, followed by selection bias and reporting bias. As to CCTs, greater risk of bias was found in the selection of participants into the study and in the measurement of outcomes. Based on the selected studies, the most commonly used aromatherapy for dental anxiety management was lavender fragrance, followed by orange. Compared to the negative control condition, aromatherapy might reduce the anxiety-related physiological parameters and psychometric rating, pain, and mood, alertness, and calmness compared to the negative control condition; and there might be no intergroup difference in such outcomes between aromatherapy and music. However, GRADEs of the relevant evidence were judged as low to very low.

Conclusions: Compared to the negative control condition, aromatherapy is more effective to reduce dental anxiety and has comparable effects on dental anxiety compared to music intervention; however, the certainty of evidence is relatively limited. Further high-quality randomised trials with robust study design and large sample size are warranted to confirm our findings and to confirm the role of aromatherapy in relieving dental anxiety.
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http://dx.doi.org/10.1111/odi.13346DOI Listing
April 2020

Long-term clinical performance of flapless implant surgery compared to the conventional approach with flap elevation: A systematic review and meta-analysis.

World J Clin Cases 2020 Mar;8(6):1087-1103

Department of Prosthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China.

Background: The conventional implant approach involves flap elevation, which may result in increased soft tissue and bone loss and postoperative morbidity. The flapless surgical technique, aided by three-dimensional medical imaging equipment, is regarded as a possible alternative to the conventional approach to alleviate the above issues. Several studies have been performed regarding the role of flapless implant surgery. However, the results are inconsistent and there is no robust synthesis of long-term evidence to better inform surgeons regarding which type of surgical technique is more beneficial to the long-term prognosis of patients in need of implant insertion.

Aim: To compare the long-term clinical performance after flapless implant surgery to that after the conventional approach with flap elevation.

Methods: PubMed, EMBASE, Cochrane Central Register of Controlled Trials, and grey literature databases were searched from inception to 23 September 2019. Randomised controlled trials (RCTs) and cohort studies comparing the long-term clinical performance after flapless implant surgery to that after the conventional approach over a follow-up of three years or more were included. Meta-analyses were conducted to estimate the odds ratios (ORs) or mean differences (MDs) and their 95% confidence intervals (CIs) between the long-term implant survival rate, marginal bone loss, and complication rate of the flapless and conventional groups. Subgroup analyses were carried out to account for the possible effects of the guided or free-hand method during flapless surgery.

Results: Ten articles, including four RCTs and six cohort studies, satisfied the eligibility criteria and nine of them were included in the meta-analysis. There was no significant difference between the long-term implant survival rate [OR = 1.30, 95%CI (0.37, 4.54), = 0.68], marginal bone loss [MD = 0.01, 95%CI (-0.42, 0.44), = 0.97], and complication rate [OR = 1.44, 95%CI (0.77, 2.68), = 0.25] after flapless implant surgery and the conventional approach. Moreover, subgroup analyses revealed that there was no statistically significant difference between the implant survival rate [guided: OR = 1.52, 95%CI (0.19, 12.35), = 0.70]; free-hand: = 1, could not be estimated), marginal bone loss [guided: MD = 0.22, 95%CI (-0.14, 0.59), = 0.23; free-hand: MD = -0.27, 95%CI (-1.10, 0.57), = 0.53], or complication rate [guided: OR = 1.16, 95%CI (0.52, 2.63), = 0.71; free-hand: OR = 1.75, 95%CI (0.66, 4.63), = 0.26] in the flapless and conventional groups either with use of the surgical guide or by the free-hand method.

Conclusion: The flapless surgery and conventional approach had comparable clinical performance over three years or more. The guided or free-hand technique does not significantly affect the long-term outcomes of flapless surgery.
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http://dx.doi.org/10.12998/wjcc.v8.i6.1087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103964PMC
March 2020

In-vivo histocompatibility and osteogenic potential of biodegradable PLDLA composites containing silica-based bioactive glass fiber.

J Biomater Appl 2020 07 31;35(1):59-71. Epub 2020 Mar 31.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

The purpose of this two-year study was to evaluate the histocompatibility and osteogenic properties of a composite material consisting of poly(l--d,l lactide) (PLDLA) and silica-based bioactive glass fibers in vivo. PLDLA and PLDLA/silica-based bioactive glass fibers pins were implanted into the erector spinae muscles and femurs of beagles. Muscle and bone tissue samples were harvested 6, 12, 16, 26, 52, 78, and 104 weeks after implantation. Histology analysis was used to assess the histocompatibility, angiogenesis, and bone-implant contact. Micro-computed tomography was used to evaluate bone formation around the pins. Immunohistochemistry and western blotting revealed the expression level of the osteogenesis-related proteins. Addition of bioactive glass was demonstrated to possess better histocompatibility and reduce the inflammatory reactions in vivo. Moreover, PLDLA/silica-based bioactive glass fibers pins were demonstrated to promote angiogenesis and increase osteogenesis-related proteins expression, and thus played a positive role in osteogenesis and osseointegration after implantation. Our findings indicated that a composite of PLDLA and silica-based bioactive glass fiber is a promising biodegradable material for clinical use.
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http://dx.doi.org/10.1177/0885328220911598DOI Listing
July 2020

Study on the substitution effects of zinc benzoate terpyridine complexes on photoluminescence, antiproliferative potential and DNA binding properties.

J Biol Inorg Chem 2020 03 28;25(2):311-324. Epub 2020 Feb 28.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, PR China.

Six zinc(II) complexes, [Zn(OCOPh)L] (R = 1, 2, 3, 4, 5, 6) were synthesized by the reaction of zinc benzoate and six para-substituted 4-phenyl-terpyridine complexes and their structures were confirmed by elemental analysis, FT-IR, H NMR and X-ray single crystal diffraction analysis. Their photoluminescent properties in solid and in solutions of DMSO were studied. Three human cancer cell lines were used for antiproliferative potential: human lung cancer cell line (A549), human esophageal cancer cell line (Eca-109) and human breast cancer cell line (MCF-7). The results have shown that these zinc complexes have good inhibitory effects on cancer cells, which are better than that of the commonly used clinical drug cisplatin. The ability of the complexes to binding to CT-DNA was studied by UV spectroscopy and fluorescence titration, while the interaction between the complexes and CT-DNA, AT6, GC6 short-chain DNA sequences and G-quadruplex were analyzed by circular dichroism (CD). It is found that these complexes can bind to DNA, and the binding mode is mainly intercalator. The docking of the complexes with the DNA fragment was simulated using molecular docking software. All the results clearly display that the substituents at these ligands of the complexes have the substitution effects on the properties of photoluminescence, antiproliferative potential and DNA binding study.
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http://dx.doi.org/10.1007/s00775-020-01763-6DOI Listing
March 2020

Long-term study on the osteogenetic capability and mechanical behavior of a new resorbable biocomposite anchor in a canine model.

J Orthop Translat 2020 Mar 14;21:81-90. Epub 2020 Jan 14.

School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.

Background: Biodegradable suture anchors are commonly used for repairing torn rotator cuffs, but these biodegradable materials still suffer from low mechanical strength, poor osteointegration, and the generation of acidic degradation byproducts.

Method: The purpose of this study was to evaluate the long-term mechanical behavior and osteogenetic capabilities of a biocomposite anchor injection molded with 30% β-tricalcium phosphate microparticles blended with 70% poly (L-lactide-co-glycolide) (85/15). This study investigated degradation and bone formation in a canine model. The initial mechanical behavior, mechanical strength retention with degradation time, and degradation features were investigated.

Results: The results showed that the biocomposite anchor had sufficient initial mechanical stability confirmed by comparing the initial shear load on the anchor with the minimum shear load borne by an ankle fracture fixation screw, which is considered a worst-case implantation site for mechanical loading. The maximum shear load retention of the biocomposite anchor was 83% at 12 weeks, which is desirable, as it aligns with the rate of bone healing. The β-tricalcium phosphate fillers were evenly dispersed in the polymeric matrix and acted to slow the degradation rate and improve the mechanical strength of the anchor. The interface characteristics between the β-tricalcium phosphate particles and the polymeric matrix changed the degradation behavior of the biocomposite. Phosphate buffer saline was shown to diffuse through the interface into the biocomposite to inhibit the core accelerated degradation rate. , the addition of β-tricalcium phosphate induced new bone formation. The biocomposite material developed in this study demonstrated improved osteogenesis in comparison to a plain poly (L-lactide-co-glycolide) material. Neither anchor produced adverse tissue reactions, indicating that the biocomposite had favorable biocompatibility following long-term implantation.

Conclusion: In summary, the new biocomposite anchor presented in this study had favorable osteogenetic capability, mechanical property, and controlled degradation rate for bone fixation.

Translational Potential Of This Article: The new biocomposite anchor had sufficient initial and long-term fixation stability and bone formation capability in the canine model. It is indicated that the new biocomposite anchor has a ​potential for orthopedic application.
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http://dx.doi.org/10.1016/j.jot.2019.12.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033359PMC
March 2020

Real Time Hand Movement Trajectory Tracking for Enhancing Dementia Screening in Ageing Deaf Signers of British Sign Language.

IFIP Adv Inf Commun Technol 2019 Aug 23;3:377-394. Epub 2019 Aug 23.

Cognitive Computing Research Lab, University of Westminster, London, UK.

Real time hand movement trajectory tracking based on machine learning approaches may assist the early identification of dementia in ageing Deaf individuals who are users of British Sign Language (BSL), since there are few clinicians with appropriate communication skills, and a shortage of sign language interpreters. Unlike other computer vision systems used in dementia stage assessment such as RGBD video with the aid of depth camera, activities of daily living (ADL) monitored by information and communication technologies (ICT) facilities, or X-Ray, computed tomography (CT), and magnetic resonance imaging (MRI) images fed to machine learning algorithms, the system developed here focuses on analysing the sign language space envelope (sign trajectories/depth/speed) and facial expression of deaf individuals, using normal 2D videos. In this work, we are interested in providing a more accurate segmentation of objects of interest in relation to the background, so that accurate real-time hand trajectories (path of the trajectory and speed) can be achieved. The paper presents and evaluates two types of hand movement trajectory models. In the first model, the hand sign trajectory is tracked by implementing skin colour segmentation. In the second model, the hand sign trajectory is tracked using Part Affinity Fields based on the OpenPose Skeleton Model [1, 2]. Comparisons of results between the two different models demonstrate that the second model provides enhanced improvements in terms of tracking accuracy and robustness of tracking. The pattern differences in facial and trajectory motion data achieved from the presented models will be beneficial not only for screening of deaf individuals for dementia, but also for assessment of other acquired neurological impairments associated with motor changes, for example, stroke and Parkinson's disease.
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http://dx.doi.org/10.1007/978-3-030-29726-8_24DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025874PMC
August 2019

Mechanical and degradative properties of PLDLA biodegradable pins with bioactive glass fibers in a beagle model.

Biomed Mater 2020 03 27;15(3):035010. Epub 2020 Mar 27.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.

The present study aimed to evaluate the mechanical and degradative properties of poly(L-co-D,L-lactic acid)/silicate bioactive glass fibers (PLDLA/SGFs) composite pins in vivo. Both PLDLA and PLDLA/SGFs pins were inserted into the erector spinae muscles and femurs of beagle dogs and were harvested 6, 12, 16, 26, 52, 78, and 104 weeks after insertion. Bone formation around the pins was evaluated by micro-computed tomography. Mechanical properties were measured by the shear strength test. Thermogravimetric analysis, differential scanning calorimetry, and gel permeation chromatography were used to assess the degradation of these materials. The surface and cross-sectional morphology of both pins were observed using a scanning electron microscope. The experimental data demonstrated that PLDLA/SGFs pins can support new bone formation due to the influence of bioactive glass fibers. PLDLA/SGFs composite pins had higher initial shear strength and were relatively stable for at least 26 weeks. The addition of bioactive glass fibers accelerated the degradation rate of the composite pins. Thus, PLDLA/SGFs composite pins have promising potential for bone fixation applications.
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http://dx.doi.org/10.1088/1748-605X/ab772dDOI Listing
March 2020

Effects of different culture systems on the culture of prepuberal buffalo () spermatogonial stem cell-like cells .

J Vet Sci 2020 Jan;21(1):e13

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Key Laboratory for Animal Reproduction and Biotechnology, College of Animal Science and Technology, Guangxi University, Nanning 530005, China.

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system ( < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, ( < 0.05), ( < 0.01) and ( < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3⁺ subset in the cells cultured in the defined materials system. The expression levels of ( < 0.05) and ( < 0.01) were significantly increased, and the expression levels of ( < 0.01) and ( < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs .
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http://dx.doi.org/10.4142/jvs.2020.21.e13DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000897PMC
January 2020

Thermal conductivity of VO nanowires and their contact thermal conductance.

Nanoscale 2020 Jan;12(2):1138-1143

School of Physics Science and Engineering, Tongji University, Shanghai 200092, China.

Vanadium pentoxide (V2O5)-based composites show outstanding performances as cathode materials in lithium-ion batteries. However, their inferior thermal conductivity restricts the heat dissipation through the cathode electrode. In this study, we measured the thermal conductivity of V2O5 nanowires using the thermal bridge method and found that their thermal conductivity is 3.84 ± 0.38 W m-1 K-1 at T = 300 K. The contact thermal resistance between two nanowires with the same size was measured to be up to 50%-80% of the total thermal resistance in the measured samples, indicating that their contact is the bottleneck for thermal dissipation.
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http://dx.doi.org/10.1039/c9nr08803bDOI Listing
January 2020

Zinc(II) Terpyridine Complexes: Substituent Effect on Photoluminescence, Antiproliferative Activity, and DNA Interaction.

Molecules 2019 Dec 10;24(24). Epub 2019 Dec 10.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China.

A series of ZnCl complexes (compounds -) with 4'-(substituted-phenyl)-2,2':6',2''-terpyridine that bears hydrogen (), -methyl (), -methoxy (), -phenyl (), -tolyl (), -hydroxyl (), -hydroxyl (), -hydroxyl (), -carboxyl (), or -methylsulfonyl () were prepared and then characterized by H NMR, electrospray mass-spectra (ESI-MS), IR, elemental analysis, and single crystal X-ray diffraction. In vitro cytotoxicity assay was used to monitor the antiproliferative activities against tumor cells. Absorption spectroscopy, fluorescence titration, circular dichroism spectroscopy, and molecular modeling studied the DNA interactions. All of the compounds display interesting photoluminescent properties and different maximal emission peaks due to the difference of the substituent groups. The cell viability studies indicate that the compounds have excellent antiproliferative activity against four human carcinoma cell lines, A549, Bel-7402, MCF-7, and Eca-109, with the lowest IC values of 0.33 (), 0.66 (), 0.37 (), and 1.05 () μM, respectively. The spectrophotometric results reveal that the compounds have strong affinity binding with DNA as intercalator and induce DNA conformational transition. Molecular docking studies indicate that the binding is contributed by the π…π stacking and hydrogen bonds, providing an order of nucleotide sequence binding selectivity as ATGC > ATAT > GCGC. These compounds intercalate into the base pairs of the DNA of the tumor cells to affect their replication and transcription, and the process is supposed to play an important role in the anticancer mechanism.
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http://dx.doi.org/10.3390/molecules24244519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943603PMC
December 2019

Hyperglycemia-induced inflamm-aging accelerates gingival senescence via NLRC4 phosphorylation.

J Biol Chem 2019 12 1;294(49):18807-18819. Epub 2019 Nov 1.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. Electronic address:

Inflamm-aging was recently affiliated with the progression of diabetic complications. Local cellular senescence together with senescence-associated secretory phenotype (SASP) are the main contributors to inflamm-aging. However, little is known about their involvement in diabetic periodontitis. Gingiva is the first line of host defense in the periodontium, and macrophages are key SASP-carrying cells. Here, we explored the molecular mechanism by which hyperglycemia drives the inflamm-aging in the gingival tissue of diabetic mice and macrophages. We demonstrated that hyperglycemia increased the infiltrated macrophage senescence in gingival tissue of diabetic mice. Simultaneously, hyperglycemia elevated the local burden of senescent cells in gingival tissue and induced the serum secretion of SASP factors Moreover, , high glucose induced macrophage senescence and SASP factors secretion through phosphorylation of NLRC4, which further stimulated the NF-κB/Caspase-1 cascade via an IRF8-dependent pathway. Deletion of NLRC4 or IRF8 abolished hyperglycemia-induced cellular senescence and SASP in macrophages. In addition, we found that treatment with metformin inhibited NLRC4 phosphorylation and remarkably decreased cellular senescence and SASP in the context of hyperglycemia. Our data demonstrated that hyperglycemia induces the development of inflamm-aging in gingival tissue and suggested that NLRC4 is a potential target for treatment of diabetes-associated complications.
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http://dx.doi.org/10.1074/jbc.RA119.010648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901307PMC
December 2019

Evaluation of different chrominance models in the detection and reconstruction of faces and hands using the growing neural gas network.

Pattern Anal Appl 2019 Nov 8;22(4):1667-1685. Epub 2019 Apr 8.

School of Computer Science and Engineering, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK.

Physical traits such as the shape of the hand and face can be used for human recognition and identification in video surveillance systems and in biometric authentication smart card systems, as well as in personal health care. However, the accuracy of such systems suffers from illumination changes, unpredictability, and variability in appearance (e.g. occluded faces or hands, cluttered backgrounds, etc.). This work evaluates different statistical and chrominance models in different environments with increasingly cluttered backgrounds where changes in lighting are common and with no occlusions applied, in order to get a reliable neural network reconstruction of faces and hands, without taking into account the structural and temporal kinematics of the hands. First a statistical model is used for skin colour segmentation to roughly locate hands and faces. Then a neural network is used to reconstruct in 3D the hands and faces. For the filtering and the reconstruction we have used the growing neural gas algorithm which can preserve the topology of an object without restarting the learning process. Experiments conducted on our own database but also on four benchmark databases (Stirling's, Alicante, Essex, and Stegmann's) and on deaf individuals from normal 2D videos are freely available on the BSL signbank dataset. Results demonstrate the validity of our system to solve problems of face and hand segmentation and reconstruction under different environmental conditions.
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http://dx.doi.org/10.1007/s10044-019-00819-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774769PMC
November 2019

Evaluation of Light Fluence Distribution Using an IR Navigation System for HPPH-mediated Pleural Photodynamic Therapy (pPDT).

Photochem Photobiol 2020 03 22;96(2):310-319. Epub 2019 Oct 22.

Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA.

Uniform light fluence distribution for patients undergoing photodynamic therapy (PDT) is critical to ensure predictable PDT outcomes. However, current practice when delivering intrapleural PDT uses a point source to deliver light that is monitored by seven isotropic detectors placed within the pleural cavity to assess its uniformity. We have developed a real-time infrared (IR) tracking camera to follow the movement of the light point source and the surface contour of the treatment area. The calculated light fluence rates were matched with isotropic detectors using a two-correction factor method and an empirical model that includes both direct and scattered light components. Our clinical trial demonstrated that we can successfully implement the IR navigation system in 75% (15/20) of the patients. Data were successfully analyzed in 80% (12/15) patients because detector locations were not available for three patients. We conclude that it is feasible to use an IR camera-based system to track the motion of the light source during PDT and demonstrate its use to quantify the uniformity of light distribution, which deviated by a standard deviation of 18% from the prescribed light dose. The navigation system will fail when insufficient percentage of light source positions is obtained (<30%) during PDT.
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http://dx.doi.org/10.1111/php.13166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093257PMC
March 2020

Synthesis, characterization, anti-tumor activity, photo-luminescence and BHb/HHb/Hsp90 molecular docking of zinc(II) hydroxyl-terpyridine complexes.

J Inorg Biochem 2019 12 22;201:110790. Epub 2019 Jul 22.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China. Electronic address:

Six zinc(II) complexes [Zn(Br)L] (1), [Zn(Br)L] (2) and [Zn(Br)L] (3), [Zn(I)L] (4), [Zn(I)L] (5) and [Zn(I)L] (6) have been obtained by the reactions of ZnBr or ZnI with p-hydroxyl-4'-phenyl-terpyridine(L), m-hydroxyl-4'-phenyl-terpyridine(L) and o-hydroxyl-4'-phenyl-terpyridine(L), which were characterized by elemental analysis, FT-IR and NMR, as well as single crystal X-ray diffraction. Comparing to cisplatin, compounds 1-6 show higher antiproliferative activity against human lung carcinoma cell line (A549), human ileocecal colorectal adenocarcinoma cell line (HCT-8) and human breast cancer cell line (MCF-7). As the concentration of compounds 1-6 increases in the BHb/HHb-compound system, the fluorescent intensity of bovine hemoglobin (BHb) and human hemoglobin (HHb) reduces with a static quenching mechanism. The binding constant and the number of binding sites for the interaction of the compounds with BHb and HHb were calculated. Molecular docking studies suggest that the binding process of these compounds with heat shock protein 90 (Hsp90), BHb and HHb is a spontaneous molecular interaction process, in which van der Waals forces and hydrogen bonds play major roles, and π-π interaction also has influence on binding process. These results suggest that compounds 1-6 can be candidates for further evaluation as chemotherapeutic agents against human tumor.
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http://dx.doi.org/10.1016/j.jinorgbio.2019.110790DOI Listing
December 2019