Publications by authors named "Xing Jin"

286 Publications

Structure modification and biological evaluation of indole-chalcone derivatives as anti-tumor agents through dual targeting tubulin and TrxR.

Eur J Med Chem 2021 Oct 5;227:113897. Epub 2021 Oct 5.

The Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, 510720, China; Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China. Electronic address:

Microtubule target agents (MTAs) are widely-used clinical anti-cancer drugs for decades, but the acquired drug resistance severely restricted their application. Thioredoxin reductases (TrxR) was reported to be overexpressed in most tumors and closely related to high risk of cancer recurrence and drug resistance, making it a potential target for anticancer drug discovery. Multi-target-directed ligands (MTDLs) by a single molecule provide a logical and alternative approach to drug combinations. In this work, based on the structure-activity relationships obtained in our previous study, some structure modifications were performed. On one hand, the retained skeleton structure of MTAs endowed its tubulin polymerization inhibition activity, on the other hand, the selenium-containing structure and α,β-unsaturated ketone moiety endowed the TrxR inhibition activity. As results, the newly obtained compounds exhibited superior anti-proliferative activities towards various human cancer cells and drug-resistance cells, and displayed high selectivity towards various human normal cells. The mechanism study revealed that the dual effect of cell cycle arrest triggered by targeting tubulin and the abnormal accumulation of ROS caused by TrxR inhibition eventually lead to cell apoptosis. Notably, compared with the MTA agents CA-4P, and the TrxR inhibitor Ethaselen, the optimized compound 14c, which served as dual-targeting inhibitor of tubulin and TrxR, exerted greatly improved in vivo anti-tumor activity. In summary, 14c deserved further consideration for cancer therapy.
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http://dx.doi.org/10.1016/j.ejmech.2021.113897DOI Listing
October 2021

Over 21% Efficiency Stable 2D Perovskite Solar Cells.

Adv Mater 2021 Oct 14:e2107211. Epub 2021 Oct 14.

Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China.

Owing to their insufficient light absorption and charge transport, 2D Ruddlesden-Popper (RP) perovskites show relatively low efficiency. In this work, methylammonium (MA), formamidinum (FA), and FA/MA mixed 2D perovskite solar cells (PSCs) are fabricated. Incorporating FA cations extends the absorption range and enhances the light absorption. Optical spectroscopy shows that FA cations substantially increase the portion of 3D-like phase to 2D phases, and X-ray diffraction (XRD) studies reveal that FA-based 2D perovskite possesses an oblique crystal orientation. Nevertheless, the ultrafast interphase charge transfer results in an extremely long carrier-diffusion length (≈1.98 µm). Also, chloride additives effectively suppress the yellow δ-phase formation of pure FA-based 2D PSCs. As a result, both FA/MA mixed and pure FA-based 2D PSCs exhibit a greatly enhanced power conversion efficiency (PCE) over 20%. Specifically, the pure FA-based 2D PSCs achieve a record PCE of 21.07% (certified at 20%), which is the highest efficiency for low-dimensional PSCs (n ≤ 10) reported to date. Importantly, the FA-based 2D PSCs retain 97% of their initial efficiency at 85 °C persistent heating after 1500 h. The results unambiguously demonstrate that pure-FA-based 2D PSCs are promising for achieving comparable efficiency to 3D perovskites, along with a better device stability.
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http://dx.doi.org/10.1002/adma.202107211DOI Listing
October 2021

Jujuboside B Inhibits the Proliferation of Breast Cancer Cell Lines by Inducing Apoptosis and Autophagy.

Front Pharmacol 2021 24;12:668887. Epub 2021 Sep 24.

Cancer Institute, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, China.

Jujuboside B (JB) is one of the main biologically active ingredients extracted from Zizyphi Spinosi Semen (ZSS), a widely used traditional Chinese medicine for treating insomnia and anxiety. Breast cancer is the most common cancer and the second leading cause of cancer-related death in women worldwide. The purpose of this study was to examine whether JB could prevent breast cancer and its underlying mechanism. First, we reported that JB induced apoptosis and autophagy in MDA-MB-231 and MCF-7 human breast cancer cell lines. Further mechanistic studies have revealed that JB-induced apoptosis was mediated by NOXA in both two cell lines. Moreover, the AMPK signaling pathway plays an important role in JB-induced autophagy in MCF-7. To confirm the anti-breast cancer effect of JB, the interaction of JB-induced apoptosis and autophagy was investigated by both pharmacological and genetic approaches. Results indicated that autophagy played a pro-survival role in attenuating apoptosis. Further study showed that JB significantly suppressed the growth of MDA-MB-231 and MCF-7 xenografts. In conclusion, our findings indicate that JB exerts its anti-breast cancer effect in association with the induction of apoptosis and autophagy.
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http://dx.doi.org/10.3389/fphar.2021.668887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497973PMC
September 2021

Integrated analysis of patients with KEAP1/NFE2L2/CUL3 mutations in lung adenocarcinomas.

Cancer Med 2021 Oct 6. Epub 2021 Oct 6.

Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Objectives: To explore the clinical features, molecular characteristics, and immune landscape of lung adenocarcinoma patients with KEAP1/NFE2L2/CUL3 mutations.

Methods: The multi-omics data from the GDC-TCGA LUAD project of The Cancer Genome Atlas (TCGA) database were downloaded from the Xena browser. The estimate of the immune infiltration was implemented by using the GSVA analysis and CIBERSORT. The status of KEAP1/NFE2L2/CUL3 mutation in 50 LUAD samples of our department was detected by using Sanger sequencing, following the relative expression level of differentially expressed genes (DEGs), miRNAs (DEmiRNAs), and lncRNAs (DElncRNAs) was validated by IHC and real-time quantitative polymerase chain reaction (RT-qPCR).

Results: The Kaplan-Meier and multivariable Cox regression analyses demonstrated that KEAP1/NFE2L2/CUL3 mutations had independent prognostic value for OS and PFS in LUAD patients. The differential analysis detected 207 upregulated genes (like GSR/UGT1A6) and 447 downregulated genes (such as PIGR). GO, KEGG, and GSEA analyses demonstrated that DEGs were enriched in glutamate metabolism and the immune response. The constructed ceRNA network shows the linkage of differential lncRNAs and mRNAs. Three hundred and nine somatic mutations were detected, alterations in immune infiltration DNA methylations and stemness scores were also founded between the two groups. Eight mutated LUAD patients were detected by Sanger DNA sequencing in 50 surgical patients. GSR and UGT1A6 were validated to express higher in the Mut group, whereas the expression of PIGR was restrained. Furthermore, the IHC staining conducted on paraffin-embedded tissue emphasizes the consistency of our result.

Conclusion: This research implemented the comprehensive analysis of KEAP1/NFE2L2/CUL3 somatic mutations in the LUAD patients. Compared with the wild type of LUAD patients, the Mut group shows a large difference in clinical features, RNA sequence, DNA methylation, and immune infiltrations, indicating complex mechanism oncogenesis and also reveals potential therapeutic targets.
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http://dx.doi.org/10.1002/cam4.4338DOI Listing
October 2021

Long non‑coding RNA AL355711 promotes smooth muscle cell migration through the ABCG1/MMP3 pathway.

Int J Mol Med 2021 Dec 5;48(6). Epub 2021 Oct 5.

Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Atherosclerosis and related cardiovascular diseases pose severe threats to human health worldwide. There is evidence to suggest that at least 50% of foam cells in atheromas are derived from vascular smooth muscle cells (VSMCs); the first step in this process involves migration to human atherosclerotic lesions. Long non‑coding RNAs (lncRNAs) have been found to play significant roles in diverse biological processes. The present study aimed to investigate the role of lncRNAs in VSMCs. The expression of lncRNAs or mRNAs was detected using reverse transcription‑quantitative polymerase chain reaction. The Gene Expression Omnibus datasets in the NCBI portal were searched using the key words 'Atherosclerosis AND tissue AND ' and the GSE12288 dataset. Gene expression in circulating leukocytes was measured to identify patients with coronary artery disease (CAD) or controls, and used to analyze the correlation coefficient and expression profiles. The protein level of ATP‑binding cassette sub‑family G member 1 (ABCG1) and matrix metalloproteinase (MMP)3 was determined using immunohistochemistry and western blot analysis. The analysis of mouse aortic roots was performed using Masson's and Oil Red O staining. The expression of lncRNA AL355711, ABCG1 and MMP3 was found to be higher in human atherosclerotic plaques or in patients with atherosclerotic CAD. The correlation analysis revealed that ABCG1 may be involved in the regulation between lncRNA AL355711 and MMP3 in atherosclerotic CAD. The knockdown of lncRNA AL355711 inhibited ABCG1 transcription and smooth muscle cell migration. In addition, lncRNA AL355711 was found to regulate MMP3 expression through the ABCG1 pathway. The expression of ABCG1 and MMP3 was found to be high in an animal model of atherosclerosis. The results indicated that lncRNA AL355711 promoted VSMC migration and atherosclerosis partly via the ABCG1/MMP3 pathway. On the whole, the present study demonstrates that the inhibition of lncRNA AL355711 may serve as a novel therapeutic target for atherosclerosis. lncRNA AL355711 in circulating leukocytes may be a novel biomarker for atherosclerotic CAD.
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http://dx.doi.org/10.3892/ijmm.2021.5040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510679PMC
December 2021

A multi-omics study delineates new molecular features and therapeutic targets for esophageal squamous cell carcinoma.

Clin Transl Med 2021 Sep;11(9):e538

Cancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Esophageal squamous cell carcinoma (ESCC) is a major histological subtype of esophageal cancer with inferior prognosis. Here, we conducted comprehensive transcriptomic, proteomic, phosphoproteomic, and metabolomic characterization of human, treatment-naive ESCC and paired normal adjacent tissues (cohort 1, n = 24) in an effort to identify new molecular vulnerabilities for ESCC and potential therapeutic targets. Integrative analysis revealed a small group of genes that were related to the active posttranscriptional and posttranslational regulation of ESCC. By using proteomic, phosphoproteomic, and metabolomic data, networks of ESCC-related signaling and metabolic pathways that were closely linked to cancer etiology were unraveled. Notably, integrative analysis of proteomic and phosphoproteomic data pinpointed that certain pathways involved in RNA transcription, processing, and metabolism were stimulated in ESCC. Importantly, proteins with close linkage to ESCC prognosis were identified. By enrolling an ESCC patient cohort 2 (n = 41), three top-ranked prognostic proteins X-prolyl aminopeptidase 3 (XPNPEP3), bromodomain PHD finger transcription factor (BPTF), and fibrillarin (FBL) were verified to have increased expression in ESCC. Among these prognostic proteins, only FBL, a well-known nucleolar methyltransferase, was essential for ESCC cell growth in vitro and in vivo. Furthermore, a validation study using an ESCC patient cohort 3 (n = 100) demonstrated that high FBL expression predicted unfavorable patient survival. Finally, common cancer/testis antigens and established cancer drivers and kinases, all of which could direct therapeutic decisions, were characterized. Collectively, our multi-omics analyses delineated new molecular features associated with ESCC pathobiology involving epigenetic, posttranscriptional, posttranslational, and metabolic characteristics, and unveiled new molecular vulnerabilities with therapeutic potential for ESCC.
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http://dx.doi.org/10.1002/ctm2.538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473482PMC
September 2021

Cardiopulmonary bypass does not consequentially contribute to postoperative distant metastasis of giant refractory thoracic tumors: A retrospective study with long-term follow-up.

Thorac Cancer 2021 Sep 16. Epub 2021 Sep 16.

Department of Thoracic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China.

Background: Few clinical research studies with long-term follow-up have revealed whether cardiopulmonary bypass (CPB) increases the risk of postoperative distant metastasis in patients with giant refractory thoracic tumors. The present study evaluated the risk of distant metastasis after surgery utilizing CPB with long-term follow-up.

Methods: Clinical data for patients with giant refractory thoracic tumors who underwent resection with the use of CPB in the Second Affiliated Hospital of Soochow University during the past 11 years were retrospectively reviewed.

Results: Of the 14 patients with giant refractory thoracic tumors who had undergone surgery under CPB, 10 patients (71.4%) were completely resected. Twelve patients were followed up for 13-127 months with 10 patients were completely resected and two patients could not be completely resected due to severe tissue invasion. Three patients (25%) suffered from distant metastasis, and four patients (33.3%) experienced local recurrence. Only one patient (1/10) with complete resection suffered from distant metastasis, while two patients (2/10) experienced local recurrence. Two patients (2/2) with major resection suffered from both distant metastasis and local recurrence. Median overall survival for patients who have been regularly followed up was 50 months with 1-, 5-, and 10-year survival of 100%, 75%, and 66.7%. No difference was found between the distant metastasis survival and the local recurrence survival. (p = 0.99).

Conclusions: CPB is an effective strategy for complete resection of the giant refractory thoracic tumors with an acceptable risk of postoperative distant metastasis for some patients.
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http://dx.doi.org/10.1111/1759-7714.14162DOI Listing
September 2021

Ablation of USP21 in skeletal muscle promotes oxidative fibre phenotype, inhibiting obesity and type 2 diabetes.

J Cachexia Sarcopenia Muscle 2021 Sep 15. Epub 2021 Sep 15.

College of Pharmacy and Integrated Research Institute for Drug Development, Dongguk University-Seoul, Goyang-si, Gyeonggi-Do, South Korea.

Background: Skeletal muscle as a metabolic consumer determines systemic energy homeostasis by regulating myofibre type conversion and muscle mass control. Perturbation of the skeletal muscle metabolism elevates the risk of a variety of diseases including metabolic disorders. However, the regulatory pathways and molecules are not completely understood. The discovery of relevant responsible molecules and the associated network could be an attractive strategy to overcome diseases associated with muscle problems.

Methods: An initial screening using quantitative trait locus analysis enabled us to extract a set of genes including ubiquitin-specific proteases21 (USP21) (r = 0.738; P = 0.004) as potential targets associated with fasting blood glucose content. Given tight regulation of the ubiquitination status of proteins in muscle, we focused on USP21 and generated whole-body (KO) and skeletal muscle-specific USP21 knockout (MKO) mice. Transcriptomics, proteomics, and lipidomics assays in combination with various in vivo and in vitro experiments were performed to understand the functions of USP21 and underlying mechanisms. A high-fat diet (60%)-fed mouse model and diabetic patient-derived samples were utilized to assess the effects of USP21 on energy metabolism in skeletal muscle.

Results: USP21 was highly expressed in both human and mouse skeletal muscle, and controlled skeletal muscle oxidative capacity and fuel consumption. USP21-KO or USP21-MKO significantly promoted oxidative fibre type changes (Δ36.6% or Δ47.2%), muscle mass increase (Δ13.8% to Δ22.8%), and energy expenditure through mitochondrial biogenesis, fatty acid oxidation, and UCP2/3 induction (P < 0.05 or P < 0.01). Consistently, cold exposure repressed USP21 expression in mouse skeletal muscle (Δ55.3%), whereas loss of USP21 increased thermogenesis (+1.37°C or +0.84°C; P < 0.01). Mechanistically, USP21 deubiquitinated DNA-PKcs and ACLY, which led to AMPK inhibition. Consequently, USP21 ablation diminished diet-induced obesity (WT vs. USP21-KO, Δ8.02 g, 17.1%, P < 0.01; litter vs. USP21-MKO, Δ3.48 g, 7.7%, P < 0.05) and insulin resistance. These findings were corroborated in a skeletal muscle-specific gene KO mouse model. USP21 was induced in skeletal muscle of a diabetic patient (1.94-fold), which was reciprocally changed to p-AMPK (0.30-fold).

Conclusions: The outcomes of this research provide novel information as to how USP21 in skeletal muscle contributes to systemic energy homeostasis, demonstrating USP21 as a key molecule in the regulation of myofibre type switch, muscle mass control, mitochondrial function, and heat generation and, thus, implicating the potential of this molecule and its downstream substrates network as targets for the treatment and/or prevention of muscle dysfunction and the associated metabolic diseases.
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http://dx.doi.org/10.1002/jcsm.12777DOI Listing
September 2021

SMG9 Serves as an Oncogene to Promote the Tumor Progression EMT and Wnt/β-Catenin Signaling Pathway in Hepatocellular Carcinoma.

Front Pharmacol 2021 12;12:701454. Epub 2021 Aug 12.

Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China.

SMG9 participates in the nonsense-mediated mRNA decay process that degrades mRNA harboring nonsense mutations introduced either at the level of transcription or RNA processing. However, little is known about the role of SMG9 in hepatocellular carcinoma (HCC). The objective of this research was to clarify the effects of SMG9 expression on HCC progression. Microarray data were acquired from NCBI Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) database to bioinformatically analyze the differential expression of SMG9 between HCC patients and normal controls. SMG9 mRNA level was measured in sixteen sets of fresh tumor tissues and adjacent non-cancerous liver tissues (ANLTs) reverse transcription-quantitative polymerase chain reaction (RT-qPCR). SMG9 protein expression was analyzed in ninety-five sets of paired formalin-fixed and paraffin-embedded tissue specimens by immunohistochemistry (IHC). In addition, clinicopathological features of SMG9 in HCC were checked. For studies, small interfering RNA (siRNA) was used to silence SMG9 expression for exploring biological functions underlying of SMG9 in SMMC-7721 and HepG2. We found that SMG9 was upregulated in HCC tissues and SMG9 levels were closely related to TNM stage, tumor number and tumor size. Cox regression and Kaplan-Meier proportional hazards analyses showed that high expression of SMG9 was associated with poor patient survival. Furthermore, proliferation, apoptosis resistance, migration and invasion of both SMMC-7721 and HepG2 cells were suppressed by SMG9 inhibition. In addition, EMT and the Wnt/β-catenin signaling pathway were involved in SMG9-mediated HCC progression. SMG9 may serve as a potential novel prognostic biomarker and therapeutic target in HCC patients.
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http://dx.doi.org/10.3389/fphar.2021.701454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397484PMC
August 2021

Comparison of yield and relative costs of different screening algorithms for tuberculosis in active case-finding: a cross-section study.

BMC Infect Dis 2021 Aug 13;21(1):813. Epub 2021 Aug 13.

National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

Background: Part of tuberculosis (TB) patients were missed if symptomatic screening was based on the main TB likely symptoms. This study conducted to compare the yield and relative costs of different TB screening algorithms in active case-finding in the whole population in China.

Methods: The study population was screened based on the TB likely symptoms through a face-to-face interview in selected 27 communities from 10 counties of 10 provinces in China. If the individuals had any of the enhanced TB likely symptoms, both chest X-ray and sputum tests were carried out for them furtherly. We used the McNemar test to analyze the difference in TB detection among four algorithms in active case-finding. Of four algorithms, two were from WHO recommendations including 1a/1c, one from China National Tuberculosis Program, and one from this study with the enhanced TB likely symptoms. Furthermore, a two-way ANOVA analysis was performed to analyze the cost difference in the performance of active case-finding adjusted by different demographic and health characteristics among different algorithms.

Results: Algorithm with the enhanced TB likely symptoms defined in this study could increase the yield of TB detection in active case-finding, compared with algorithms recommended by WHO (p < 0.01, Kappa 95% CI: 0. 93-0.99) and China NTP (p = 0.03, Kappa 95% CI: 0.96-1.00). There was a significant difference in the total costs among different three algorithms WHO 1c/2/3 (F = 59.13, p < 0.01). No significant difference in the average costs for one active TB case screened and diagnosed through the process among Algorithms 1c/2/3 was evident (F = 2.78, p = 0.07). The average costs for one bacteriological positive case through algorithm WHO 1a was about two times as much as the costs for one active TB case through algorithms WHO 1c/2/3.

Conclusions: Active case-finding based on the enhanced symptom screening is meaningful for TB case-finding and it could identify more active TB cases in time. The findings indicated that this enhanced screening approach cost more compared to algorithms recommend by WHO and China NTP, but the increased yield resulted in comparative costs per patient. And it cost much more that only smear/bacteriological-positive TB cases are screened in active case-finding.
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http://dx.doi.org/10.1186/s12879-021-06486-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361931PMC
August 2021

Learning Dynamic Relationships for Facial Expression Recognition Based on Graph Convolutional Network.

IEEE Trans Image Process 2021 12;30:7143-7155. Epub 2021 Aug 12.

Facial action units (AUs) analysis plays an important role in facial expression recognition (FER). Existing deep spectral convolutional networks (DSCNs) have made encouraging performance for FER based on a set of facial local regions and a predefined graph structure. However, these regions do not have close relationships to AUs, and DSCNs cannot model the dynamic spatial dependencies of these regions for estimating different facial expressions. To tackle these issues, we propose a novel double dynamic relationships graph convolutional network (DDRGCN) to learn the strength of the edges in the facial graph by a trainable weighted adjacency matrix. We construct facial graph data by 20 regions of interest (ROIs) guided by different facial AUs. Furthermore, we devise an efficient graph convolutional network in which the inherent dependencies of vertices in the facial graph can be learned automatically during network training. Notably, the proposed model only has 110K parameters and 0.48MB model size, which is significantly less than most existing methods. Experiments on four widely-used FER datasets demonstrate that the proposed dynamic relationships graph network achieves superior results compared to existing light-weight networks, not just in terms of accuracy but also model size and speed.
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http://dx.doi.org/10.1109/TIP.2021.3101820DOI Listing
August 2021

Identification of a Seven-lncRNA-mRNA Signature for Recurrence and Prognostic Prediction in Relapsed Acute Lymphoblastic Leukemia Based on WGCNA and LASSO Analyses.

Anal Cell Pathol (Amst) 2021 9;2021:6692022. Epub 2021 Jun 9.

Department of Hematology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310014, China.

Abnormal expressions of long noncoding RNAs (lncRNAs) and protein-encoding messenger RNAs (mRNAs) are important for the development of childhood acute lymphoblastic leukemia (ALL). This study developed an lncRNA-mRNA integrated classifier for the prediction of recurrence and prognosis in relapsed childhood ALL by using several transcriptome data. Weighted gene coexpression network analysis revealed that green, turquoise, yellow, and brown modules were preserved across the TARGET, GSE60926, GSE28460, and GSE17703 datasets, and they were associated with clinical relapse and death status. A total of 184 genes in these four modules were differentially expressed between recurrence and nonrecurrence samples. Least absolute shrinkage and selection operator analysis showed that seven genes constructed a prognostic signature (including one lncRNA: LINC00652 and six mRNAs: INSL3, NIPAL2, REN, RIMS2, RPRM, and SNAP91). Kaplan-Meier curve analysis observed that patients in the high-risk group had a significantly shorter overall survival than those of the low-risk group. Receiver operating characteristic curve analysis demonstrated that this signature had high accuracy in predicting the 5-year overall survival and recurrence outcomes, respectively. LINC00652 may function by coexpressing with the above prognostic genes (INSL3, SNAP91, and REN) and lipid metabolism-related genes (MIA2, APOA1). Accordingly, this lncRNA-mRNA-based classifier may be clinically useful to predict the recurrence and prognosis for childhood ALL. These genes represent new targets to explain the mechanisms for ALL.
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http://dx.doi.org/10.1155/2021/6692022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208884PMC
June 2021

Prevalence of Hepatitis E Virus Infection among Laboratory Rabbits in China.

Pathogens 2021 Jun 21;10(6). Epub 2021 Jun 21.

Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing 102629, China.

Hepatitis E virus (HEV) is zoonotic and the leading cause of acute viral hepatitis worldwide. Rabbit HEV can infect humans and is prevalent globally. It is reported that laboratory rabbits are also naturally infected with HEV. Therefore, it is important to investigate in a large scale the prevalence of HEV in laboratory rabbits. Serum samples were collected from 649 laboratory rabbits of 13 different commercial vendors in Beijing, China, from 2017 to 2019, and anti-HEV and HEV antigen (Ag) were tested. Fecal samples were collected from 50 laboratory rabbits from one of the vendors for HEV RNA detection. Six laboratory rabbits with natural HEV infection were euthanized and their liver, kidney, bile and urine samples were collected for HEV RNA quantification. Liver tissues were subjected to histopathology analysis. The overall positive rates of anti-HEV antibodies and HEV-Ag are 2.6% (15/588) and 7.9% (51/649), respectively. HEV RNA was detected in 12.0% (6/50) of the rabbits. High viral load of HEV RNA was detected in liver and bile samples. Liver inflammation was observed. HEV is circulating in laboratory rabbit population in China. Strict screening is crucial to ensure experimental accuracy and prevent zoonotic transmission to research personnel.
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http://dx.doi.org/10.3390/pathogens10060780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233994PMC
June 2021

Metabolic mechanisms of a drug revealed by distortion-free C tracer analysis.

Chem Sci 2021 Feb 24;12(13):4958-4962. Epub 2021 Feb 24.

Natural Product Research Institute, College of Pharmacy, Seoul National University 1 Gwanak-ro, Gwanak-gu Seoul 08826 Korea

Metabolomic isotopic tracing can provide flux information useful for understanding drug mechanisms. For that, NMR has the unique advantage of giving positional isotope enrichment information, but the current C 1D NMR approach suffers from low sensitivity and high overlaps. We developed a new 2D heteronuclear NMR experiment incorporating -scaling and distortion-free elements that allows for quantitative analysis of multiplets with high sensitivity and resolution. When applied to an old chemotherapeutic drug, the approach provided a quantitative estimation of TCA-cycle turns, confirming the conventional mechanism of its mitochondrial metabolic enhancement. Additionally, the approach identified a new mechanism of the higher contribution of the pentose phosphate pathway to serine synthesis in the cytosolic compartment, possibly explaining the broad pharmacological activities of the drug. Our approach may prove beneficial in helping to find new usages or metabolic mechanisms of other drugs.
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http://dx.doi.org/10.1039/d0sc06480gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179602PMC
February 2021

Bone Autophagy: A Potential Way of Exercise-Mediated Meg3/P62/Runx2 Pathway to Regulate Bone Formation in T2DM Mice.

Diabetes Metab Syndr Obes 2021 17;14:2753-2764. Epub 2021 Jun 17.

Rehabilitation Medicine Department, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou City, Jiangsu Province, People's Republic of China.

Background: Meg3 has been shown to attenuate T2DM bone autophagy by activating p62 to inhibit bone formation. However, whether exercise can reverse this process to promote T2DM bone formation and its mechanism remains unknown.

Methods: A T2DM mouse model was established by a high-fat diet and STZ injection, and the mice were trained with 8-week HIIT and downhill running exercise. Micro-CT was used to scan the bone microstructure. Bone morphology was observed by HE staining, and the osteoblast (OB) activity in bones was observed by AKP staining. Calcium ion and phosphorus concentration in serum was detected by ELISA; RT-PCR was used to detect the mRNA level, and Western blot was used to detect the protein level of related indexes in Meg3/p62/Runx2 pathway.

Results: The inhibition of bone autophagy, in the bones of T2DM mice, resulted in the degradation of the bone tissue morphology and structure, with the increase of the expressions of Meg3, PI3K, Akt, mTOR, p62 and NF-κB. However, 8-week HIIT and downhill running could reverse this process, especially downhill running, manifested with the up-regulation of miR-16 mRNA level, along with Beclin-1, LC3 II and Runx2 mRNA and protein level.

Conclusion: T2DM leads to pathology in model mice. Eight-week HIIT and downhill running exercise can inhibit Meg3, activate autophagy of osteoblasts and promote bone formation in T2DM mice.
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http://dx.doi.org/10.2147/DMSO.S299744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216663PMC
June 2021

Propofol Anesthesia Decreased the Efficiency of Long-Range Cortical Interaction in Humans.

IEEE Trans Biomed Eng 2021 Jun 23;PP. Epub 2021 Jun 23.

Objective: Phase-amplitude coupling (PAC) has recently been used to illuminate cross-frequency coordination in neurophysiological activity of electroencephalogram. However, the PAC at a meso-scale (electrocorticogram, ECoG) and PAC between different areas have still not been fully clarified.

Methods: In this study, we analyzed ECoG data recorded from surgical patients (n= 9) with pharmaco-resistant epilepsy during the surgical treatment. The modulogram and a genuine modulation index, based on a Kullback-Leibler distance and permutation test method, were developed and used to measure the slow oscillation (SO) (0.15-1 Hz)- (8-13 Hz) PAC of within-lead and cross-lead during transitions from states of wakefulness to unconsciousness during propofol induced general anesthesia.

Results: In within-lead SO- PAC, the modulation index increased in the unconscious state (p<0.05, Tukeys test), the percentages of genuine modulation indices also increased in the unconscious state (p<0.001 in the frontal area and p<0.01 in the parietal area), and distinct PAC patterns emerged more often. In cross-lead SO- PAC, there are fewer PAC patterns compared to within-lead, and the percentages of genuine modulation indices decreased significantly (p<0.001).

Conclusion: The increased modulation index of within-lead and cross-lead SO- PAC is associated with a reduction of information integration and the efficiency of long distance synchronization. These findings demonstrate that the propofol causes the neuronal populations to enter a busy state in a local scale, which prevents the information integration in long-range areas.
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http://dx.doi.org/10.1109/TBME.2021.3090027DOI Listing
June 2021

Improving the outcomes from electroactive constructed wetlands by mixing wastewaters from different beverage-processing industries.

Chemosphere 2021 Nov 15;283:131203. Epub 2021 Jun 15.

College of Geography and Environment, Shandong Normal University, Jinan, 250014, PR China. Electronic address:

Denitrification in electroactive constructed wetland (EW) systems is constrained by the carbon source and the carbon/nitrogen (C/N) ratio (the COD/TN ratio). In this study, wastewater with a high C/N from a brewery was added to wastewater with a low C/N (dairy wastewater) in an EW system, and the pollutant removal, bioelectricity generation, transformations of dissolved organic matter, and microbial community structures were evaluated. The results showed that the average removal rates of ammonium nitrogen, total nitrogen, and chemical oxygen demand from the wastewater mixture were 6.40%, 46.44%, and 23.85% higher than those from the wastewater with a low C/N, respectively. Dissimilatory nitrate reduction to ammonium was effectively inhibited, and the NH-N removal was 25.52% higher, when the wastewater mixture was used instead of the high C/N wastewater. Similarly, the output voltage was significantly increased, and the internal resistance of the device was reduced, for the wastewater mixture. The structure of the microbial community improved, the relative abundance of electrochemically active bacteria was higher, and the protein-like and humic-like components were lower, in the mixture treatment than in the individual treatment. The results show that the nitrogen removal and biopower generation improved in an EW system when high C/N wastewater was used as the carbon source.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131203DOI Listing
November 2021

[Application of Two Ultrafine Chest Tube for Drainage after
Pulmonary Uniportal Video-assisted Thoracoscopic Surgery].

Zhongguo Fei Ai Za Zhi 2021 Aug 17;24(8):577-582. Epub 2021 Jun 17.

Department of Cardiothoracic Surgery, the Second Affiliated Hospital, Soochow University, Suzhou 215004, China.

Background: At present, an ultrafine chest tube combined with a traditional thick tube were often used after pulmonary uniportal video-assisted thoracoscopic surgery (U-VATS). However, the thick tube was often placed in the incision, which increased the risk of poor wound healing and postoperative pain. The aim of this study is to investigate the feasibility and safety of using two ultrafine chest tubes (10 F pigtail tube) for drainage after pulmonary U-VATS.

Methods: The medical records of patients who underwent pulmonary U-VATS during June 2018 and June 2020 in the department of cardiothoracic surgery of the second affiliated hospital of Soochow university were retrospectively reviewed to compare two different drainage strategies, receiving two 10 F pigtail tubes as chest tube (group A) or one 10 F pigtail tube as lower chest tube combined with one 24 F tube as upper chest tube (group B).

Results: 106 patients in group A receiving two 10 F pigtail tubes during June 2019 and June 2020 and 183 patients in group B receiving one 10 F pigtail tube as lower chest tube combined with one 24 F tube as upper chest tube during June 2018 and June 2019 were included. There was no significant difference between two groups in terms of the postoperative thoracic drainage (mL) (1st: 199.54±126.56 vs 203.59±139.32, P=0.84; 2nd: 340.30±205.47 vs 349.74±230.92, P=0.76; 3rd: 435.19±311.51 vs 451.37±317.03, P=0.70; 4th: 492.58±377.33 vs 512.57±382.94, P=0.69; Total: 604.57±547.24 vs 614.64±546.08, P=0.88), drainage time (d) (upper chest tube: 2.54±2.20 vs 3.40±2.07, P=0.21; lower chest tube: (2.24±2.43 vs 3.82±2.12, P=0.10), postoperative hospital stays (d) (6.87±3.17 vs 7.06±3.21, P=0.63), poor wound healing (0 vs 3.28%, P=0.09), replacement of lower chest tube (0.94% vs 2.19%, P=0.66) and the VAS1 (3.00±0.24 vs 2.99±0.15, P=0.63). Notably, there were significant differences between two groups in terms of the VAS₂ (2.28±0.63 vs 2.92±0.59, P<0.01) and VAS₃ (2.50±1.58 vs 2.79±1.53, P=0.02), as well as the frequency of using additional analgesics (25.47% vs 38.25%, P=0.03) and replacement of the upper chest tube (0 vs 4.37%, P=0.03).

Conclusions: It's feasible and safe to use two 10 F pigtail tubes for drainage after pulmonary U-VATS, which can achieve less postoperative pain and lower frequency of replacement of the upper chest tube on some specific patients.
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http://dx.doi.org/10.3779/j.issn.1009-3419.2021.101.23DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387650PMC
August 2021

Lactic acid bacteria that activate immune gene expression in Caenorhabditis elegans can antagonise Campylobacter jejuni infection in nematodes, chickens and mice.

BMC Microbiol 2021 06 5;21(1):169. Epub 2021 Jun 5.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.

Background: Campylobacter jejuni is the major micro-bacillary pathogen responsible for human coloenteritis. Lactic acid bacteria (LAB) have been shown to protect against Campylobacter infection. However, LAB with a good ability to inhibit the growth of C. jejuni in vitro are less effective in animals and animal models, and have the disadvantages of high cost, a long cycle, cumbersome operation and insignificant immune response indicators. Caenorhabditis elegans is increasingly used to screen probiotics for their anti-pathogenic properties. However, no research on the use of C. elegans to screen for probiotic candidates antagonistic to C. jejuni has been conducted to date.

Results: This study established a lifespan model of C. elegans, enabling the preselection of LAB to counter C. jejuni infection. A potential protective mechanism of LAB was identified. Some distinct LAB species offered a high level of protection to C. elegans against C. jejuni. The LAB strains with a high protection rate reduced the load of C. jejuni in C. elegans. The transcription of antibacterial peptide genes, MAPK and Daf-16 signalling pathway-related genes was elevated using the LAB isolates with a high protection rate. The reliability of the lifespan model of C. elegans was verified using mice and chickens infected with C. jejuni.

Conclusions: The results showed that different LAB had different abilities to protect C. elegans against C. jejuni. C. elegans provides a reliable model for researchers to screen for LAB that are antagonistic to C. jejuni on a large scale.
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http://dx.doi.org/10.1186/s12866-021-02226-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180125PMC
June 2021

Identification of important proteins from the gonads and pituitary involved in the gonad development of Amur sturgeon, Acipenser schrenckii, regulated by GnRH-a treatment by iTRAQ-based analysis.

Comp Biochem Physiol Part D Genomics Proteomics 2021 09 31;39:100831. Epub 2021 Mar 31.

Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, China. Electronic address:

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http://dx.doi.org/10.1016/j.cbd.2021.100831DOI Listing
September 2021

TruthTrust: Truth Inference-Based Trust Management Mechanism on a Crowdsourcing Platform.

Sensors (Basel) 2021 Apr 7;21(8). Epub 2021 Apr 7.

School of Cyberspace, Hangzhou Dianzi University, Hangzhou 310018, China.

On a crowdsourcing platform, in order to cheat for rewards or sabotage the crowdsourcing processes, spam workers may submit numerous erroneous answers to the tasks published by requesters. This type of behavior extremely reduces the completion rate of tasks and the enthusiasm of honest users, which may lead a crowdsourcing platform to a failure. Defending against malicious attacks is an important issue in crowdsourcing, which has been extensively addressed by existing methods, e.g., verification-based defense mechanisms, data analysis solutions, trust-based defense models, and workers' properties matching mechanisms. However, verification-based defense mechanisms will consume a lot of resources, and data analysis solutions cannot motivate workers to provide high-quality services. Trust-based defense models and workers' properties matching mechanisms cannot guarantee the authenticity of information when collusion requesters publish shadow tasks to help malicious workers get more participation opportunities. To defend such collusion attacks in crowdsourcing platforms, we propose a new defense model named TruthTrust. Firstly, we define a complete life cycle system that from users' interaction to workers' recommendation, and separately define the trust value of each worker and the credence of each requester. Secondly, in order to ensure the authenticity of the information, we establish a trust model based on the CRH framework. The calculated truth value and weight are used to define the global properties of workers and requesters. Moreover, we propose a reverse mechanism to improve the resistance under attacks. Finally, extensive experiments demonstrate that TruthTrust significantly outperforms the state-of-the-art approaches in terms of effective task completion rate.
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http://dx.doi.org/10.3390/s21082578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067557PMC
April 2021

A Self-Healing Flexible Quasi-Solid Zinc-Ion Battery Using All-In-One Electrodes.

Adv Sci (Weinh) 2021 Apr 14;8(8):2004689. Epub 2021 Feb 14.

National Laboratory of Solid State Microstructures College of Engineering and Applied Sciences Nanjing University Nanjing Jiangsu 210093 P. R. China.

Self-healing and flexibility are significant for many emerging applications of secondary batteries, which have attracted broad attention. Herein, a self-healing flexible quasi-solid Zn-ion battery composing of flexible all-in-one cathode (VS nanosheets growing on carbon cloth) and anode (electrochemically deposited Zn nanowires), and a self-healing hydrogel electrolyte, is presented. The free-standing all-in-one electrodes enable a high capacity and robust structure during flexible transformation of the battery, and the hydrogel electrolyte possesses a good self-healing performance. The presented battery remains as a high retention potential even after healing from being cut into six pieces. When bending at 60°, 90°, and 180°, the battery capacities remain 124, 125, and 114 mAh g, respectively, cycling at a current density of 50 mA g. Moreover, after cutting and healing twice, the battery still delivers a stable capacity, indicating a potential use of self-healing and wearable electronics.
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http://dx.doi.org/10.1002/advs.202004689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061350PMC
April 2021

Integration of single-cell and bulk RNA sequencing data reveals key cell types and regulators in traumatic brain injury.

Math Biosci Eng 2021 01;18(2):1201-1214

Department of Neurosurgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China.

Traumatic brain injury (TBI) is a leading cause of disability and mortality worldwide, whose symptoms ranging from mild to severe, even life-threatening. However, specific cell types and key regulators involved in traumatic brain injury have not been well elucidated. In this study, utilizing single-cell RNA-seq (scRNA-seq) data from mice with TBI, we have successfully identified and characterized 13 cell populations including astrocytes, oligodendrocyte, newly formed oligodendrocytes, microglia, two types of endothelial cells, five types of excitatory and two types of inhibitory neurons. Differential expression analysis and gene set enrichment analysis (GSEA) revealed the upregulation of microglia and endothelial markers, along with the downregulation of markers of excitatory neurons in TBI. The cell-cell communication analysis revealed that microglia and endothelial cell might interact through the interaction of Icam1-Il2rg and C1qa-Cd93, and microglia might also communicate with each other via Icam1-Itagm. The autocrine ligand-receptor in microglia might result in activation of TYROBP causal network via Icam1-Itgam. The cell-cell contact between microglia and endothelial cell might activate integrin signaling pathways. Moreover, we also found that genes involved in microglia activation were highly downregulated in Tyrobp/Dap12-deficient microglia, indicating that the upregulation of Tyrobp and TYROBP causal network in microglia might be a candidate therapeutic target in TBI. In contrast, the excitatory neurons were involved in maintaining normal brain function, and their inactivation might cause dysfunction of nervous system in TBI patients. In conclusion, the present study has discerned major cell types such as microglia, endothelial cells and excitatory neurons, and revealed key regulator such as TYROBP, C1QA, and CD93 in TBI, which shall improve our understanding of the pathogenesis of TBI.
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http://dx.doi.org/10.3934/mbe.2021065DOI Listing
January 2021

Per- and polyfluoroalkyl substances and their alternatives in paper food packaging.

Compr Rev Food Sci Food Saf 2021 05 7;20(3):2596-2625. Epub 2021 Mar 7.

World Centric, Rohnert Park, California, USA.

Per- and polyfluoroalkyl substances (PFAS) have been used in food contact paper and paperboard for decades due to their unique ability to provide both moisture and oil/grease resistance. Once thought to be innocuous, it is now clear that long chain PFAS bioaccumulate and are linked to reproductive and developmental abnormalities, suppressed immune response, and tumor formation. Second-generation PFAS have shorter biological half-lives but concerns about health risks from chronic exposure underscore the need for safe substitutes. Waxes and polymer film laminates of polyethylene, poly(ethylene-co-vinyl alcohol), and polyethylene terephthalate are commonly used alternatives. However, such laminates are neither compostable nor recyclable. Lamination with biodegradable polymers, including polyesters, such as polylactic acid (PLA), polybutylene adipate terephthalate, polybutylene succinate, and polyhydroxyalkanoates, are of growing research and commercial interest. PLA films are perhaps the most viable alternative, but performance and compostability are suboptimal. Surface sizings and coatings of starches, chitosan, alginates, micro- and nanofibrilated cellulose, and gelatins provide adequate oil barrier properties but have poor moisture resistance without chemical modification. Plant proteins, including soy, wheat gluten, and corn zein, have been tested as paper coatings with soy being the most commercially important. Internal sizing agents, such as alkyl ketene dimers, alkenyl succinic anhydride, and rosin, improve moisture resistance but are poor oil/grease barriers. The difficulty in finding a viable replacement for PFAS chemicals that is cost-effective, fully biodegradable, and environmentally sound underscores the need for more research to improve barrier properties and process economics in food packaging products.
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http://dx.doi.org/10.1111/1541-4337.12726DOI Listing
May 2021

Response of macroinvertebrate communities to land use and water quality in Wudalianchi Lake.

Ecol Evol 2021 Feb 7;11(3):1368-1377. Epub 2021 Jan 7.

Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences Harbin China.

Macroinvertebrate assemblages are structured by a number of abiotic and biotic factors interacting simultaneously. We investigated macroinvertebrate assemblages along gradients of human disturbance and morphometric characteristics in five lakes connected by the same stream. We aimed to assess the relative effects of environmental gradients on macroinvertebrate assemblages and to investigate whether water quality effects on the assemblages were correlated with buffer land use. There were significant differences in macroinvertebrate community compositions among lakes, and our results indicated that oligochaetes (mainly s) and insects (mainly ) contributed highly to the differences. We used redundancy analysis with variation partitioning to quantify the independent and combined anthropogenic effects of water quality and land use gradients on the macroinvertebrate community. The independent effect of water quality was responsible for 17% of the total variance in macroinvertebrate community composition, the independent effect of buffer land use accounted for 6% of variation, and the combined variation between land use change and water quality accounted for 12%. Our study indicated that both the independent effects of land use and within-lake water quality can explain the influence in macroinvertebrate assemblages, with significant interactions between the two. This is rather important to notice that changes in buffer land use generally may alter nutrient inputs and thus severely affect abiotic conditions encountered by macroinvertebrate. Our study demonstrates that considering buffer zone effects explicitly may be significant in the selection and application of conservation and management strategies.
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http://dx.doi.org/10.1002/ece3.7140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863386PMC
February 2021

A Signature of Five Long Non-Coding RNAs for Predicting the Prognosis of Alzheimer's Disease Based on Competing Endogenous RNA Networks.

Front Aging Neurosci 2020 28;12:598606. Epub 2021 Jan 28.

Department of Orthopedic Surgery, School of Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China.

Long non-coding RNAs (lncRNAs) play important roles in the pathogenesis of Alzheimer's disease (AD). However, the functions and regulatory mechanisms of lncRNA are largely unclear. Herein, we obtained 3,158 lncRNAs by microarray re-annotation. A global network of competing endogenous RNAs (ceRNAs) was developed for AD and normal samples were based on the gene expressions profiles. A total of 255 AD-deficient messenger RNA (mRNA)-lncRNAs were identified by the expression correlation analysis. Genes in the dysregulated ceRNAs were found to be mainly enriched in transcription factors and micro RNAs (miRNAs). Analysis of the disordered miRNA in the lncRNA-mRNA network revealed that 40 pairs of lncRNA shared more than one disordered miRNA. Among them, nine lncRNAs were closely associated with AD, Parkinson's disease, and other neurodegenerative diseases. Of note, five lncRNAs were found to be potential biomarkers for AD. Real-time quantitative reverse transcription PCR (qRT-PCR) assay revealed that PART1 was downregulated, while SNHG14 was upregulated in AD serum samples when compared to normal samples. This study elucidates the role of lncRNAs in the pathogenesis of AD and presents new lncRNAs that can be exploited to design diagnostic and therapeutic agents for AD.
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http://dx.doi.org/10.3389/fnagi.2020.598606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876075PMC
January 2021

Towards an End-to-End Framework of CCTV-Based Urban Traffic Volume Detection and Prediction.

Sensors (Basel) 2021 Jan 18;21(2). Epub 2021 Jan 18.

Australian Urban Research Infrastructure Network (AURIN), The University of Melbourne, Corner Swanston and Elgin Street, Perth, VIC 3010, Australia.

Near real-time urban traffic analysis and prediction are paramount for effective intelligent transport systems. Whilst there is a plethora of research on advanced approaches to study traffic recently, only one-third of them has focused on urban arterials. A ready-to-use framework to support decision making in local traffic bureaus using largely available IoT sensors, especially CCTV, is yet to be developed. This study presents an end-to-end urban traffic volume detection and prediction framework using CCTV image series. The framework incorporates a novel Faster R-CNN to generate vehicle counts and quantify traffic conditions. Then it investigates the performance of a statistical-based model (SARIMAX), a machine learning (random forest; RF) and a deep learning (LSTM) model to predict traffic volume 30 min in the future. Tests at six locations with varying traffic conditions under different lengths of past time series are used to train the prediction models. RF and LSTM provided the most accurate predictions, with RF being faster than LSTM. The developed framework has been successfully applied to fill data gaps under adverse weather conditions when data are missing. It can be potentially implemented in near real time at any CCTV location and integrated into an online visualization platform.
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http://dx.doi.org/10.3390/s21020629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830990PMC
January 2021

Relationship between TSHR, BRAF and PIK3CA gene copy number variations and thyroid nodules.

Endocrine 2021 07 11;73(1):116-124. Epub 2021 Jan 11.

Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang, China.

Purpose: This study aims to investigate the relationship between the TSHR, BRAF, and PIK3CA gene copy number variations (CNVs) and thyroid nodules by analyzing gene CNVs, and to explore the interaction between iodine status and the above genes CNVs in the occurrence of thyroid nodules.

Methods: Three hundred and ninety-five subjects were selected from 3 regions with different iodine status in Shanxi Province of China, including 192 patients with thyroid nodules and 203 healthy controls. The basic information about subjects had been obtained through a questionnaire. B ultrasound was utilized to check thyroid nodules. Blood and urine samples were harvested to detect the thyroid function and urinary iodine concentration. Real-time quantitative polymerase chains reaction (RT-PCR) served to detect CNVs in DNA from human blood.

Results: There was an association between TSHR gene CNV and thyroid nodules (χ = 8.403, P = 0.004). The prevalence of BRAF and PIK3CA gene CNVs was not statistically significant between the case group and the control group. Differences in the TSHR gene CNV rates for cases of the 3 areas were statistically significant (χ = 10.072, P = 0.007). No statistical difference in the prevalence rates of the 3 genes CNVs between diverse characteristics of thyroid nodules was observed. UIC > 300 μg/L (OR = 1.74, 95% CI: 1.02-2.96, P = 0.041) and TSHR gene CNV (OR = 3.53, 95% CI: 1.40-8.92, P = 0.008) were risk factors for thyroid nodules. There was no significant interaction between the UIC and the examined genes CNVs.

Conclusions: TSHR gene CNV and high urinary iodine levels can increase the risk of thyroid nodules. But the interactions between the 3 above genes CNVs and iodine nutrition were not found in the occurrence of thyroid nodules.
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http://dx.doi.org/10.1007/s12020-020-02587-9DOI Listing
July 2021

[Trophic niche and interspecific diet relationship of in autumn from Langcuo Lake of Tibet, China.]

Ying Yong Sheng Tai Xue Bao 2020 Dec;31(12):4284-4290

Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haerbin 150070, China.

Langcuo Lake is a typical plateau lake in the southern Tibet, with an altitude of 4300 m. and coexisted in this lake. In this study, we investigated the food composition and trophic niche of two fishes in autumn by carbon and nitrogen stable isotopes technique in 2018. We further analyzed their interspecific diet relationship, including the diet similarity index and overlap coefficient. The results showed that mainly feed on ephemeroptera larvae, chironomidae larvae, periphytic algae, gammarus and plankton, while mainly feed on ephemeroptera larvae, periphytic algae, chironomidae larvae, gammarus and aquatic vascular plants. Two fishes had trophic niche overlap. The δC range value (CR=1.60), δN range value (NR=1.89), and total area of convex hull (TA=1.80) of were lower than those of , with the difference values being 0.09, 0.57 and 1.29, respectively. The diet similarity index and overlap coefficient of those two species were higher, with a value of 94.2% and 91.5%, respectively. Our results suggested that the interspecific diet competition may be intense between the and in Langcuo Lake in autumn.
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http://dx.doi.org/10.13287/j.1001-9332.202012.034DOI Listing
December 2020

Complete mitochondrial genome sequence of in the Yarlung Zangbo River, Tibet.

Mitochondrial DNA B Resour 2020 Jan 7;5(1):368-369. Epub 2020 Jan 7.

Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, P. R. China.

belongs to the family of Sisoridae, . It is distributed in the Yarlung Zangbo River, the Irrawaddy River, the Nujiang River, and the Lancang River in southwestern China. In this study, we first published the complete mitochondrial genome sequence of , which was 16,589 bp in length. This genome consists of 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a non-coding A + T-rich region. The PCGs start with a traditional ATG except for COX1 and NAD3, which start with GTG and ATA instead, respectively, and end with stop codon TAA, TAG, TA, or a single T base. All tRNA have the typical clover-leaf structure. The phylogenetic tree of the whole mitogenome sequence is constructed by using neighbor-joining (NJ) method and the phylogenetic relationship among the family Sisoridae is further analyzed. We except to provide the theoretical basis for the further study of the phylogenetic relationship, taxonomic status, and conservation and management of genetic resources of Sisoridae catfishes.
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http://dx.doi.org/10.1080/23802359.2019.1703576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748630PMC
January 2020
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