Publications by authors named "Xing Fan"

436 Publications

Fractional microneedle radiofrequency device and fractional erbium-doped glass 1,565-nm device treatment of human facial photoaging: a prospective, split-face, random clinical trial.

J Cosmet Laser Ther 2022 Jan 27:1-7. Epub 2022 Jan 27.

Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.

Microneedle fractional radiofrequency (MFR) and non-ablative 1565 nm fractional laser (NAFL) have recently been introduced as new techniques to address the growing concern of facial photoaging. In this prospective randomized split-face study, we wanted to compare the safety and efficacy of MFR with that of NAFL for the treatment of facial photoaging in Asian patients. Fifteen healthy Chinese patients were enrolled for this randomized split-face study. Each patient underwent three sessions of treatment with MFR and NAFL on opposite sides of their face, one month apart. A blinded outcome assessment of the photoaging severity was performed by two independent plastic surgeons on a 5-point visual analogue scale (VAS, 0-4). Patient satisfaction was also scored based on a 5-point VAS (0 = dissatisfaction, 4 = extremely satisfied). Sagging of the nasolabial groove was evaluated using the Antera 3D camera, facial wrinkles and pores using the VISIA skin analysis system. Any adverse events that occurred during the study were also evaluated. Based on the VAS scores and results from the Antera 3D and VISIA, it was noted that there was a significant improvement in facial skin laxity, wrinkles, and pores, and lesser sagging of the nasolabial groove on both the MFR and NAFL sides of the face, compared with that of the baseline. Most patients were satisfied with the treatment and reported tolerable pain and crusting. Although no significant differences were observed between the MFR and NAFL treatments, the NAFL treatment resulted in a shorter downtime(4.56 ± 2.72d) than the MFR treatment(6.96 ± 3.27d). This study confirms the efficacy of MFR and NAFL treatments for facial skin rejuvenation in Asian patients. Furthermore, the therapies were found to be safe and well-tolerated. Our findings suggest that NAFL may be a more convenient treatment modality for facial photoaging because of its shorter downtime. However, sagging of the nasolabial groove was more improved by the MFR treatment than by the NAFL treatment.
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http://dx.doi.org/10.1080/14764172.2022.2033783DOI Listing
January 2022

Immune Dysfunctions of CD56 NK Cells Are Associated With HIV-1 Disease Progression.

Front Immunol 2021 7;12:811091. Epub 2022 Jan 7.

Department of Infectious Diseases, the Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing, China.

Background: Populations of natural killer cells lacking CD56 expression [CD56 natural killer (NK) cells] have been demonstrated to expand during human immunodeficiency virus (HIV)-1 infection. However, their phenotypic and functional characteristics have not been systematically analyzed, and their roles during disease progression remain poorly understood.

Methods: In this study, 84 donors, namely 34 treatment-naïve HIV-1-infected patients (TNs), 29 HIV-1-infected patients with successful antiretroviral therapy (ARTs), and 21 healthy controls (HCs), were enrolled. The phenotypic and functional characteristics of CD56 NK cells were analyzed using single-cell RNA-sequencing (scRNA-seq) and flow cytometry. A potential link between the characteristics of CD56 NK cells and the clinical parameters associated with HIV-1 disease progression was examined.

Results: The frequency of the CD56 NK cell population was significantly increased in TNs, which could be partially rescued by ART. Flow cytometry analyses revealed that CD56 NK cells were characterized by high expression of CD39, TIGIT, CD95, and Ki67 compared to CD56 NK cells. assays revealed reduced IFN-γ and TNF-α secretion, as well as decreased expression of granzyme B and perforin in CD56 NK cells. In line with the data obtained by flow cytometry, scRNA-seq analysis further demonstrated impaired cytotoxic activities of CD56 NK cells. Notably, a negative correlation was observed between CD39, CD95, and Ki67 expression levels in CD56 NK cells and CD4 T cell counts.

Conclusions: The results presented in this study indicate that the CD56 NK cell population expanded in HIV-1-infected individuals is dysfunctional and closely correlates with HIV-1 disease progression.
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http://dx.doi.org/10.3389/fimmu.2021.811091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777256PMC
January 2022

A novel phosphorylation site in SARS-CoV-2 nucleocapsid regulates its RNA-binding capacity and phase separation in host cells.

J Mol Cell Biol 2022 Jan 18. Epub 2022 Jan 18.

Centre for Infection and Immunity Study (CIIS), School of Medicine, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518197, China.

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http://dx.doi.org/10.1093/jmcb/mjac003DOI Listing
January 2022

MicroRNA profile as potential molecular signature for attention deficit hyperactivity disorder in children.

Biomarkers 2022 Jan 6:1-27. Epub 2022 Jan 6.

Department of Clinical Medicine, Hangzhou Medical College, Zhejiang, P.R. China.

Aims: Attention deficit/hyperactivity disorder (ADHD) is a prevalent disorder of neurodevelopment in children. The diagnosis of ADHD mainly relies on the symptoms and some may be misdiagnosed due to age-based variation in behaviours. This study aimed to explore biomarkers that are greatly needed for the accurate diagnosis of ADHD.

Methods: 742 samples were retrospectively investigated in 3 independent cohorts, screening, training, and validation, for circulation microRNA measurement using microarray, Taqman polymerase chain reaction and regression analysis.

Results: A panel of five miRNAs (miR-4516, miR-6090, miR-4763-3p, miR-4281 and miR-4466) were identified as ADHD independent risk factors that provided a high diagnostic accuracy and specificity of ADHD (AUC =0.940 and 0.927 in the training and validation datasets, respectively). This panel of miRNAs differentiated ADHD well from control groups. After clinical improvement by treatment, the panel of miRNAs in patients and AUC changed significantly, and were close to those in healthy controls. Importantly, the targets of the miRNAs identified were commonly enriched in receptor signalling pathways, ion channels and synapse structures.

Conclusion: Our study identified a useful panel of miRNAs that have considerable clinical value in evaluating ADHD and provide important evidence for aberrant epigenetic regulation in ADHD.
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http://dx.doi.org/10.1080/1354750X.2021.2024600DOI Listing
January 2022

Increased Platelet-CD4 T Cell Aggregates Are Correlated With HIV-1 Permissiveness and CD4 T Cell Loss.

Front Immunol 2021 20;12:799124. Epub 2021 Dec 20.

Medical School of Chinese People's Liberation Army of China (PLA), Beijing, China.

Chronic HIV-1 infection is associated with persistent inflammation, which contributes to disease progression. Platelet-T cell aggregates play a critical role in maintaining inflammation. However, the phenotypic characteristics and clinical significance of platelet-CD4 T cell aggregates remain unclear in different HIV-infected populations. In this study, we quantified and characterized platelet-CD4 T cell aggregates in the peripheral blood of treatment-naïve HIV-1-infected individuals (TNs), immunological responders to antiretroviral therapy (IRs), immunological non-responders to antiretroviral therapy (INRs), and healthy controls (HCs). Flow cytometry analysis and immunofluorescence microscopy showed increased platelet-CD4 T cell aggregate formation in TNs compared to HCs during HIV-1 infection. However, the frequencies of platelet-CD4 T cell aggregates decreased in IRs compared to TNs, but not in INRs, which have shown severe immunological dysfunction. Platelet-CD4 T cell aggregate frequencies were positively correlated with HIV-1 viral load but negatively correlated with CD4 T cell counts and CD4/CD8 ratios. Furthermore, we observed a higher expression of CD45RO, HIV co-receptors, HIV activation/exhaustion markers in platelet-CD4 T cell aggregates, which was associated with HIV-1 permissiveness. High levels of caspase-1 and caspase-3, and low levels of Bcl-2 in platelet-CD4 T cell aggregates imply the potential role in CD4 T cell loss during HIV-1 infection. Furthermore, platelet-CD4 T cell aggregates contained more HIV-1 gag viral protein and HIV-1 DNA than their platelet-free CD4 T cell counterparts. The platelet-CD4 T cell aggregate levels were positively correlated with plasma sCD163 and sCD14 levels. Our findings demonstrate that platelet-CD4 T cell aggregate formation has typical characteristics of HIV-1 permissiveness and is related to immune activation during HIV-1 infection.
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http://dx.doi.org/10.3389/fimmu.2021.799124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720770PMC
December 2021

Implications of the accumulation of CXCR5 NK cells in lymph nodes of HIV-1 infected patients.

EBioMedicine 2021 Dec 29;75:103794. Epub 2021 Dec 29.

Senior Department of Infectious Diseases, the Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing, China. Electronic address:

Background: B cell follicles are immune-privileged sites where intensive HIV-1 replication and latency occur, preventing a permanent cure. Recent study showed that CXCR5 NK cells in B cell follicles can inhibit SIV replication in African green monkeys, but this has not been reported in HIV-1 infected patients.

Methods: Lymphocytes and tissue sections of lymph node were collected from 11 HIV-1 positive antiretroviral therapy (ART)-naive and 19 HIV-1 negative donors. We performed immunofluorescence and RNA-scope to detect the location of CXCR5 NK cells and its relationship with HIV-1 RNA, and performed flow cytometry and RNA-seq to analyze the frequency, phenotypic and functional characteristics of CXCR5 NK cells. The CXCL13 expression were detected by immunohistochemistry.

Findings: CXCR5 NK cells, which accumulated in LNs from HIV-1 infected individuals, expressed high levels of activating receptors such as NKG2D and NKp44. CXCR5 NK cells had upregulated expression of CD107a and β-chemokines, which were partially impaired in HIV-1 infection. Importantly, the frequency of CXCR5NK cells was inversely related to the HIV-1 viral burden in LNs. In addition, CXCL13-the ligand of CXCR5-was upregulated in HIV-1 infected individuals and positively correlated with the frequency of CXCR5 NK cells.

Interpretation: During chronic HIV-1 infection, CXCR5 NK cells accumulated in lymph node, exhibit altered immune characteristics and underlying anti-HIV-1 effect, which may be an effective target for a functional cure of HIV-1.
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http://dx.doi.org/10.1016/j.ebiom.2021.103794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728057PMC
December 2021

Variation in Plastome Sizes Accompanied by Evolutionary History in Monogenomic Triticeae (Poaceae: Triticeae).

Front Plant Sci 2021 13;12:741063. Epub 2021 Dec 13.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

To investigate the pattern of chloroplast genome variation in Triticeae, we comprehensively analyzed the indels in protein-coding genes and intergenic sequence, gene loss/pseudonization, intron variation, expansion/contraction in inverted repeat regions, and the relationship between sequence characteristics and chloroplast genome size in 34 monogenomic Triticeae plants. Ancestral genome reconstruction suggests that major length variations occurred in four-stem branches of monogenomic Triticeae followed by independent changes in each genus. It was shown that the chloroplast genome sizes of monogenomic Triticeae were highly variable. The chloroplast genome of , , , , , , , and in Triticeae had evolved toward size reduction largely because of pseudogenes elimination events and length deletion fragments in intergenic. The / complex, , , , , and in Triticeae had a larger chloroplast genome size. The large size variation in major lineages and their subclades are most likely consequences of adaptive processes since these variations were significantly correlated with divergence time and historical climatic changes. We also found that several intergenic regions, such as N-C and E-L containing unique genetic information, which can be used as important tools to identify the maternal relationship among Triticeae species. Our results contribute to the novel knowledge of plastid genome evolution in Triticeae.
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http://dx.doi.org/10.3389/fpls.2021.741063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710740PMC
December 2021

Identification of a Wheat- 7Ns Ditelosomic Addition Line Conferring Early Maturation by Cytological Analysis and Newly Developed Molecular and FISH Markers.

Front Plant Sci 2021 9;12:784001. Epub 2021 Dec 9.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Early maturation is an important objective in wheat breeding programs that could facilitate multiple-cropping systems, decrease disaster- and disease-related losses, ensure stable wheat production, and increase economic benefits. Exploitation of novel germplasm from wild relatives of wheat is an effective means of breeding for early maturity. Keng f. ex P. C. KUO (2=2=14, NsNs) is a promising source of useful genes for wheat genetic improvement. In this study, we characterized a novel wheat- line, DT23, derived from distant hybridization between common wheat and . Fluorescence hybridization (FISH) and sequential genomic hybridization (GISH) analyses indicated that DT23 is a stable wheat- ditelosomic addition line. FISH painting and PCR-based landmark unique gene markers analyses further revealed that DT23 is a wheat- 7Ns ditelosomic addition line. Observation of spike differentiation and the growth period revealed that DT23 exhibited earlier maturation than the wheat parents. This is the first report of new earliness () gene(s) probably associated with a group 7 chromosome of . Based on specific locus-amplified fragment sequencing technology, 45 new specific molecular markers and 19 specific FISH probes were developed for the 7Ns chromosome. Marker validation analyses revealed that two specific markers distinguished the Ns genome chromosomes of and the chromosomes of other wheat-related species. These newly developed FISH probes specifically detected Ns genome chromosomes of in the wheat background. The DT23 line will be useful for breeding early maturing wheat. The specific markers and FISH probes developed in this study can be used to detect and trace chromosomes and chromosomal segments carrying elite genes in diverse materials.
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http://dx.doi.org/10.3389/fpls.2021.784001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695443PMC
December 2021

Prediction of Post-operative Visual Deterioration Using Visual-Evoked Potential Latency in Extended Endoscopic Endonasal Resection of Craniopharyngiomas.

Front Neurol 2021 3;12:753902. Epub 2021 Dec 3.

Department of Neurophysiology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.

The current study aimed to investigate the predictive value of visual-evoked potential (VEP) latency for post-operative visual deterioration in patients undergoing craniopharyngioma resection via extended endoscopic endonasal approach (EEEA). Data from 90 patients who underwent craniopharyngioma resection EEEA with intraoperative VEP monitoring were retrospectively reviewed. P100 latency was compared between patients with and without post-operative visual deterioration, and the threshold value of P100 latency for predicting post-operative visual deterioration was calculated by the receiver operating characteristic curve analysis. In addition, other potential prognostic factors regarding post-operative visual outcomes were also analyzed by multivariate analysis. Patients with post-operative visual deterioration showed a significantly longer VEP latency than those without ( < 0.001). An extension over 8.61% in VEP latency was identified as a predictor of post-operative visual deterioration ( < 0.001). By contrast, longer preoperative visual impairment duration and larger tumor volume were not significant predictors for post-operative visual deterioration. The current study revealed that intraoperative VEP monitoring in EEEA is effective for predicting post-operative visual deterioration, and an extension over 8.61% in VEP latency can be used as a critical cut-off value to predict post-operative visual deterioration.
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http://dx.doi.org/10.3389/fneur.2021.753902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678070PMC
December 2021

Intraoperative motor and somatosensory evoked potential monitoring during surgical clipping of ruptured and unruptured intracranial aneurysms: a comparative study.

J Neurosurg 2021 Nov 26:1-8. Epub 2021 Nov 26.

Objective: The current study investigated the correlation between intraoperative motor evoked potential (MEP) and somatosensory evoked potential (SSEP) monitoring and both short-term and long-term motor outcomes in aneurysm patients treated with surgical clipping. Moreover, the authors provide a relatively optimal neurophysiological predictor of postoperative motor deficits (PMDs) in patients with ruptured and unruptured aneurysms.

Methods: A total of 1017 patients (216 with ruptured aneurysms and 801 with unruptured aneurysms) were included. Patient demographic characteristics, clinical features, intraoperative monitoring data, and follow-up data were retrospectively reviewed. The efficacy of using changes in MEP/SSEP to predict PMDs was assessed using binary logistic regression analysis. Subsequently, receiver operating characteristic curve analysis was performed to determine the optimal critical value for duration of MEP/SSEP deterioration.

Results: Both intraoperative MEP and SSEP monitoring were significantly effective for predicting short-term (p < 0.001 for both) and long-term (p < 0.001 for both) PMDs in aneurysm patients. The critical values for predicting short-term PMDs were amplitude decrease rates of 57.30% for MEP (p < 0.001 and area under the curve [AUC] 0.732) and 64.10% for SSEP (p < 0.001 and AUC 0.653). In patients with an unruptured aneurysm, the optimal critical values for predicting short-term PMDs were durations of deterioration of 17 minutes for MEP (p < 0.001 and AUC 0.768) and 21 minutes for SSEP (p < 0.001 and AUC 0.843). In patients with a ruptured aneurysm, the optimal critical values for predicting short-term PMDs were durations of deterioration of 12.5 minutes for MEP (p = 0.028 and AUC 0.706) and 11 minutes for SSEP (p = 0.043 and AUC 0.813).

Conclusions: The authors found that both intraoperative MEP and SSEP monitoring are useful for predicting short-term and long-term PMDs in patients with unruptured and ruptured aneurysms. The optimal intraoperative neuromonitoring method for predicting PMDs varies depending on whether the aneurysm has ruptured or not.
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http://dx.doi.org/10.3171/2021.8.JNS21479DOI Listing
November 2021

A randomized, split-face controlled trial on the safety and effects of microneedle fractional radiofrequency and fractional erbium-doped glass 1,565-nm laser therapies for baggy lower eyelids.

J Cosmet Laser Ther 2021 Nov 23:1-8. Epub 2021 Nov 23.

Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, SN, China.

The non-ablative fractional erbium-doped glass 1,565-nm laser (NAFL) and the microneedle fractional radiofrequency (MFR) procedures are effective treatments that enable periorbital skin rejuvenation. To compare the clinical effectiveness and side effects of MFR and the NAFL for baggy lower eyelids (BLEs) in the Chinese population. Fifteen Chinese subjects with BLEs received three split-face treatments on a monthly basis randomly. Objective and subjective assessments were performed at baseline, as well as 1 month and 3 months after the third treatment. The results were evaluated using Antera-3D and CineScan systems. Blinded investigator assessments were performed by two plastic surgeons using a 0 to 4 score in six anatomic categories of BLEs. The patients also reported their level of satisfaction based on a four-point score. Most of the patients reported a greater than 47% satisfaction rate with both treatments. The cumulative contribution scores of prolapse of orbital fat, hollow tear trough, and skin laxity for each category variable declined with time. Using Antera 3D, the volume of elevation (mm) decreased from 0.6 ± 0.4 to 0.4 ± 0.3 and from 0.6 ± 0.3 to 0.3 ± 0.3, the elevation area (mm) decreased from 17.0 ± 8.4 to 13.0 ± 7.1 and from 17.0 ± 7.8 to 10.0 ± 5.6, and the maximum peak height (mm) also decreased from 0.10 ± 0.04 to 0.06 ± 0.04 and from 0.10 ± 0.03 to 0.06 ± 0.02 in the MFR and NAFL groups, respectively. Using CineScan, the depth of middle orbital fat (mm) decreased significantly from 10.2 ± 2.2 to 8.0 ± 0.7 and from 9.8 ± 1.1 to 8.0 ± 0.9 and the length of orbital fat significantly decreased from 9.2 ± 1.2 to 7.7 ± 0.7 and from 9.7 ± 1.4 to 7.8 ± 0.6 in the MFR and NAFL groups, respectively. MFR and NAFL therapies were effective for the treatment of BLEs, especially in BLE patients with skin elasticity in addition to tear trough deformity and orbital fat prolapse. Trial registration number: NCT04237324. Trial register: ClinicalTrials.gov. Level of Evidence: Level I, therapeutic study.
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http://dx.doi.org/10.1080/14764172.2021.2001532DOI Listing
November 2021

Enhanced degradation capability of white-rot fungi after short-term pre-exposure to silver ion: Performance and selectively antimicrobial mechanisms.

Sci Total Environ 2021 Nov 15:151672. Epub 2021 Nov 15.

College of Civil Engineering, Fuzhou University, Fuzhou 350116, China.

Azo dyes in wastewater have great threats to environment and human health. White-rot fungi (WRF) have broad-spectrum potential for such refractory organics bioremediation; however, their applications are largely restrained by the poor viability owning to microbial invasion under non-sterile conditions. In this study, short-term pre-exposure to silver ion (Ag) was demonstrated to be a practical, economic, and green method to enhance the perdurability of azo dyes decoloration by WRF Phanerochaete chrysosporium under non-sterile conditions. In control (without Ag pre-exposure), decoloration deactivated since cycle 7 (<10%), whereas in Ag pre-exposure groups, the decoloration ratios remained 91.5%-94.7% after 7 cycles. Variations in decoloration-related extracellular lignin enzyme activities were consistent with the decoloration effectiveness. The enhanced decoloration capability in Ag pre-exposure groups under non-sterile conditions could be ascribed to the selectively antimicrobial action by Ag. The released Ag from the self-assembled silver nanoparticles (AgNPs) could selectively "stimulate" the proliferation and viability of P. chrysosporium, and simultaneously inhibit the growths of invasive microorganisms. The pyrosequencing results indicated that genus Sphingomonas (24.1%-31.3%) was the main invasive bacteria in Ag pre-exposure groups after long-term operation owing to the AgNPs passivation. As control, the invasive fungi (Asterotremella humicola) and bacteria (Burkholderia spp.) occurred in control after short-term operation, and genus Burkholderia (74.9%) dominated after long-term operation, leading to decoloration deactivation. Overall, these findings offer a new insight into the bio-nano interactions between WRF and invasive microorganisms in response to Ag or biogenic AgNPs, and could extend WRF application perspective under non-sterile conditions in future.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151672DOI Listing
November 2021

Development and validation of prognostic scoring in primary intestinal diffuse large B-cell lymphoma: a single-institution study of 184 patients.

Ann Transl Med 2021 Oct;9(20):1542

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: The incidence of primary intestinal diffuse large B-cell lymphoma (PI-DLBCL) is much lower than primary gastric DLBCL, and large-scale analyses on the clinical characteristics, molecular features, therapeutic strategies, and risk stratification have been seldomly performed in PI-DLBCL.

Methods: To assess prognostic model development, 107 PI-DLBCL patients diagnosed before 2014 were studied for prognosis factors including different primary involved sites and treatment strategies. For internal validation, a non-random split sample set with 77 PI-DLBCL patients after 2014 was included for validation of the prognosis factors.

Results: Patients with an ileocecal lesion presented with better survival time than those with non-ileocecal sites, with surgical resection significantly influencing the prognosis. Non-ileocecal patients who underwent surgery with lymphadenectomy had superior overall survival (OS) and progression-free survival (PFS) compared to those receiving surgery without lymphadenectomy or those not receiving (without) surgery. For ileocecal patients, surgery with or without lymphadenectomy resulted in better OS and PFS than those without surgery. For biomarker analysis, only BCL-2 >50% or Ki67 >80% on tumor cells indicated poor clinical outcome. In multivariate analysis, age, Eastern Cooperative Oncology Group (ECOG) score, and site of origin were independent prognostic factors for inferior OS in PI-DLBCL. A prognosis model was set up based on age, ECOG score, and site of origin, and validated well.

Conclusions: The prognosis in patients with PI-DLBCL with ileocecal involvement showed was better than those with non-ileocecal involvement. Surgical strategy can impact the clinical outcome of PI-DLBCL patients.
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http://dx.doi.org/10.21037/atm-21-4761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576653PMC
October 2021

Functional Group Characteristics and Pyrolysis/Combustion Performance of Karamay OS Based on FT-IR and TG-DTG Analyses.

ACS Omega 2021 Oct 11;6(42):27684-27696. Epub 2021 Oct 11.

State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources and Key Laboratory of Coal Clean Conversion & Chemical Engineering Process (Xinjiang Uyghur Autonomous Region), College of Chemical Engineering, Xinjiang University, Urumqi, Xinjiang 830046, China.

Proximate analysis, ultimate analysis, Fourier-transform infrared spectroscopy (FT-IR), and thermogravimetry-differential thermal analysis characterization were carried out on oily sludge (OS) samples OS1-OS5, from Karamay, Xinjiang, China. The Coast-Redfern model (CRm) was used to simulate the pyrolysis and combustion kinetics of oily samples. The results showed that the peak area percentage of benzene ring trisubstitution of OS5, in the range of 700-900 cm, is close to 75%, corresponding to its high volatile content. Based on the kinetic analysis by the CRm, it is found that the fitting degree of the five samples is better when the reaction order is selected as = 2, with close to 1.00 and 2/ to 0. Among them, the and of OS5 are 17.8 × 10% min °C and 0.10899 × 10% min °C, respectively, higher than those of other samples, indicating a good combustion performance.
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http://dx.doi.org/10.1021/acsomega.1c02734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552240PMC
October 2021

HIV-1-Specific CD11c CD8 T Cells Display Low PD-1 Expression and Strong Anti-HIV-1 Activity.

Front Immunol 2021 15;12:757457. Epub 2021 Oct 15.

Senior Department of Infectious Diseases, Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing, China.

Exhaustion of HIV-1-specific CD8 T cells prevents optimal control of HIV-1 infection. Identifying unconventional CD8 T cell subsets to effectively control HIV-1 replication is vital. In this study, the role of CD11c CD8 T cells during HIV-1 infection was evaluated. The frequencies of CD11c CD8 T cells significantly increased and were negatively correlated with viral load in HIV-1-infected treatment-naïve patients. HIV-1-specific cells were enriched more in CD11c CD8 T cells than in CD11c CD8 T cells, which could be induced by HIV-1-derived overlapping peptides, marking an HIV-1-specific CD8 T cell population. This subset expressed higher levels of activating markers (CD38 and HLA-DR), cytotoxic markers (granzyme B, perforin, and CD107a), and cytokines (IL-2 and TNF-α), with lower levels of PD-1 compared to the CD11c CD8 T cell subset. In vitro analysis verified that CD11c CD8 T cells displayed a stronger HIV-1-specific killing capacity than the CD11c counterparts. These findings indicate that CD11c CD8 T cells have potent immunotherapeutic efficacy in controlling HIV-1 infection.
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http://dx.doi.org/10.3389/fimmu.2021.757457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554207PMC
October 2021

Enhanced thermophilic denitrification performance and potential microbial mechanism in denitrifying granular sludge system.

Bioresour Technol 2022 Jan 26;344(Pt A):126190. Epub 2021 Oct 26.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China. Electronic address:

Thermophilic biological nitrogen removal will provide low-cost strategies for the treatment of high-temperature nitrogenous wastewater (greater than 45 ℃). In this study, a thermophilic denitrifying granular sludge system was established at 50 ℃ and compared with mesophilic systems (30 ℃ and 40 ℃). The results showed a significant increase in COD and nitrate removal rate with the elevating temperature. Besides, the microbial community analysis indicated an obvious succession of key functional bacteria at different temperatures. Enriched thermophiles including Truepera, Azoarcus, and Elioraea were the dominant denitrifiers in the thermophilic denitrifying granular sludge system, which ensured the high nitrate removal at 50 ℃. Moreover, the functional gene prediction also denoted an enrichment of nitrate reduction genes and carbon metabolism pathways at 50 ℃, which could explain the enhancement of thermophilic denitrification. These findings could provide new insight into the application of denitrifying granular sludge in thermophilic wastewater treatment.
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http://dx.doi.org/10.1016/j.biortech.2021.126190DOI Listing
January 2022

AURKA facilitates the psoriasis-related inflammation by impeding autophagy-mediated AIM2 inflammasome suppression.

Immunol Lett 2021 12 25;240:98-105. Epub 2021 Oct 25.

Department of Dermatology and Venereology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China; Institute of Dermatology, Anhui Medical University, Hefei, Anhui, 230022, China.

Psoriasis is an immune-mediated genetic disease involving innate and the adaptive immune system. Aurora kinase A (AURKA) belongs to a seine/threonine kinases family and is elevated in lesional psoriatic tissues. This research aimed to investigate the effects of AURKA on psoriasis progression and whether it worked by regulating autophagy or inflammasome activation. The results showed that the expression of AURKA was higher in psoriasis tissue than that in the psoriasis skin. IFN-γ (100 ng/mL) plus poly (dA:dT) (2 mg/mL) induced the increased AURKA, secretion of IL-1β, IL-18 and the active form of caspase-1 (p20). AURKA knockdown inhibited the inflammatory responses of keratinocytes and the activation of AIM2 inflammasome, and enhanced autophagy. 3MA (autophagy inhibitor) attenuated the effects of AURKA on AIM2 inflammasome. In addition, AURKA promoted the activation of the AKT/mTOR pathway. Akt inhibitor (PI-103) attenuated AIM2 inflammasome activation induced by Aurka overexpression. In conclusion, this research demonstrated that AURKA promoted the psoriasis-related inflammation by blocking autophagy-mediated AIM2 inflammasome suppression. AURKA has the potential to be explored as a new promising target for the treatment for psoriasis.
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http://dx.doi.org/10.1016/j.imlet.2021.10.004DOI Listing
December 2021

Development and Identification of a Novel Wheat-Thinopyrum scirpeum 4E (4D) Chromosomal Substitution Line with Stripe Rust and Powdery Mildew Resistance.

Plant Dis 2021 Oct 26. Epub 2021 Oct 26.

Sichuan Agricultural University, Triticeae Research Institute, Chengdu, Sichuan, China, 611130;

Stripe rust caused by Puccinia striiformis f. sp. tritici and powdery mildew caused by Blumeria graminis f. sp. tritici are devastating diseases of wheat worldwide. Exploration of new disease-resistant genes from cultivated wheat and wild relatives are the most effective means of reducing the amounts of fungicides applied to combat these diseases. Thinopyrum scirpeum (2n = 4x = 28, EEEE) is an important promising reservoir of useful genes, including stripe rust and powdery mildew resistance, and may be useful for enhancing wheat disease resistance. Here, we characterize a novel wheat-Th. scirpeum disomic substitution line, K16-730-3, and chromosome-specific markers were developed that can be used to trace the Th. scirpeum chromosome or chromosome segments transferred into wheat. Genomic in situ hybridization and fluorescence in situ hybridization analyses indicated that K16-730-3 is a new 4E (4D) chromosomal substitution line. Evaluation of seedling and adult disease responses revealed that K16-730-3 is resistant to stripe rust and powdery mildew. In addition, no obvious difference in grain yield was observed between K16-730-3 and its wheat parents. Genotyping-by-sequencing analyses indicated that 74 polymerase chain reaction -based markers can accurately trace chromosome 4E which were linked to the disease resistance genes in the wheat background. Further marker validation analyses revealed that 13 specific markers can distinguish between the E-genome chromosomes of Th. scirpeum and the chromosomes of other wheat-related species. The new substitution line K16-730-3 carrying the stripe rust and powdery mildew resistance genes will be useful as novel germplasm in breeding for disease resistance. The markers developed in this study can be used in marker-assisted selection for improvement of disease resistance in wheat.
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http://dx.doi.org/10.1094/PDIS-08-21-1599-REDOI Listing
October 2021

Fusion Genes Altered in Adult Malignant Gliomas.

Front Neurol 2021 4;12:715206. Epub 2021 Oct 4.

Department of Neurosurgery, University of Minnesota, Minneapolis, MN, United States.

Malignant gliomas are highly heterogeneous brain tumors in molecular genetic background. Despite the many recent advances in the understanding of this disease, patients with adult high-grade gliomas retain a notoriously poor prognosis. Fusions involving oncogenes have been reported in gliomas and may serve as novel therapeutic targets to date. Understanding the gene fusions and how they regulate oncogenesis and malignant progression will contribute to explore new approaches for personalized treatment. By now, studies on gene fusions in gliomas remain limited. However, some current clinical trials targeting fusion genes have presented exciting preliminary findings. The aim of this review is to summarize all the reported fusion genes in high-grade gliomas so far, discuss the characterization of some of the most popular gene fusions occurring in malignant gliomas, as well as their function in tumorigenesis, and the underlying clinical implication as therapeutic targets.
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http://dx.doi.org/10.3389/fneur.2021.715206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520976PMC
October 2021

Characterization of the Durum Wheat- 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance.

Front Plant Sci 2021 30;12:745290. Epub 2021 Sep 30.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Durum wheat is one of the important food and cash crops. The main goals in current breeding programs are improving its low yield potential, kernel characteristics, and lack of resistance or tolerance to some biotic and abiotic stresses. In this study, a nascent synthesized hexaploid wheat Lanmai/AT23 is used as the female parent in crosses with its AB genome donor Lanmai. A tetraploid line YL-443 with supernumerary spikelets and high resistance to stripe rust was selected out from the pentaploid F progeny. Somatic analysis using multicolor fluorescence hybridization (mc-FISH) revealed that this line is a disomic substitution line with the 4B chromosome pair of Lanmai replaced by the 4D chromosome pair of AT23. Comparing with Lanmai, YL-443 shows an increase in the number of spikelets and florets per spike by 36.3 and 75.9%, respectively. The stripe rust resistance gene carried on the 4D chromosome was fully expressed in the tetraploid background. The present 4D(4B) disomic substitution line YL-443 was distinguished from the previously reported 4D(4B) lines with the 4D chromosomes from Chinese Spring (CS). Our study demonstrated that YL-443 can be used as elite germplasm for durum wheat breeding targeting high yield potential and stripe rust resistance. The -specific PCR marker and the 4D chromosome-specific KASP markers together with its unique features of pubescent leaf sheath and auricles can be utilized for assisting selection in breeding.
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http://dx.doi.org/10.3389/fpls.2021.745290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514839PMC
September 2021

Correction to: Tumor location-based classification of surgery-related language impairments in patients with glioma.

J Neurooncol 2021 Nov;155(2):153

Beijing Neurosurgical Institute, Capital Medical University, 119, the Western Road of the southern 4th Ring Road, Beijing, 100070, China.

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http://dx.doi.org/10.1007/s11060-021-03871-yDOI Listing
November 2021

TpIRT1 from Polish wheat (Triticum polonicum L.) enhances the accumulation of Fe, Mn, Co, and Cd in Arabidopsis.

Plant Sci 2021 Nov 16;312:111058. Epub 2021 Sep 16.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China. Electronic address:

Uptake and internal transport of micronutrients are essential for plant growth, development, and yield. In this regard, Iron Regulated Transporters (IRTs) from the Zinc Regulated Transporter (ZRT)/IRT-related protein (ZIP) family play an important role in transition metal uptake. Most studies have been focused on IRT1-like proteins in diploid species. Information on IRT1-like proteins in polyploids is limited. Here, we studied the function of TpIRT1A and TpIRT1B homoeologs in a tetraploid crop, Polish wheat (Triticum polonicum L.). Our results highlighted the importance of TpIRT1 in mediating the uptake and translocation of Fe, Mn, Co, and Cd with direct implications for wheat yield potential. Both TpIRT1A and TpIRT1B were located at the plasma membrane and internal vesicle-like organelle in protoplasts of Arabidopsis thaliana L. and increased Cd and Co sensitivity in yeast. The over-expression of TpIRT1B in A. thaliana increased Fe, Mn, Co, and Cd concentration in its tissues and improved plant growth under Fe, Mn, and Co deficiencies, while increased the sensitivity to Cd compared to wild type. Functional analysis of IRT1 homoeologs from tetraploid and diploid ancestral wheat species in yeast disclosed four distinct amino acid residues in TdiIRT1B (T. dicoccum L. (Schrank)) and TtuIRT1B (T. turgidum L.). Together, our results increase the knowledge of IRT1 function in a globally important crop, wheat.
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http://dx.doi.org/10.1016/j.plantsci.2021.111058DOI Listing
November 2021

SIRT1 Regulates Tau Expression and Tau Synaptic Pathology.

J Alzheimers Dis 2021 ;84(2):895-904

Department of Biochemistry and Molecular Biology, Medical School, Nantong University, Nantong, Jiangsu, P.R. China.

Background: Amyloid plaques and neurofibrillary tangles are two pathological hallmarks of Alzheimer's disease (AD). However, synaptic deficits occur much earlier and correlate stronger with cognitive decline than amyloid plaques and neurofibrillary tangles. Mislocalization of tau is an early hallmark of neurodegeneration and precedes aggregations. Sirtuin type 1 (SIRT1) is a deacetylase which acts on proteins including transcriptional factors and associates closely with AD.

Objective: The present study investigated the association between SIRT1 and tau expression/tau localization in cells and in mice brains.

Methods: Western blot was performed to detected tau, SIRT1, C/EBPα, and GAPDH protein levels. Immunological fluorescence assay was used to assess tau localization in primary cortical neuronal cells. Golgi staining was performed to evaluated dendritic spine morphology in mice brains.

Results: In the present study, we found that SIRT1 negatively regulates expression of tau at the transcriptional level through transcriptional factor C/EBPα. Inhibition of the activity of SIRT1 limits the distribution of tau to the neurites. In the meantime, the alteration of dendritic spine morphology is also observed in the brains of SIRT1+/- mice.

Conclusion: SIRT1 may be a potential drug target for early intervention in AD.
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http://dx.doi.org/10.3233/JAD-215118DOI Listing
January 2022

Tumor location-based classification of surgery-related language impairments in patients with glioma.

J Neurooncol 2021 Nov 1;155(2):143-152. Epub 2021 Oct 1.

Beijing Neurosurgical Institute, Capital Medical University, 119, the Western Road of the southern 4th Ring Road, Beijing, 100070, China.

Introduction: Many patients with glioma experience surgery-related language impairment. This study developed a classification system to predict postoperative language prognosis.

Methods: Sixty-eight patients were retrospectively reviewed. Based on their location, tumors were subtyped as follows: (I) inferior frontal lobe or precentral gyrus; (II) posterior central gyrus or supramarginal gyrus (above the lateral fissure level); (III) posterior region of the superior or middle temporal gyri or supramarginal gyrus (below the lateral fissure level); and (IV) insular lobe. The distance from the tumor to the superior longitudinal fasciculus/arcuate fasciculus was calculated. The recovery of language function was assessed using the Western Aphasia Battery before surgery, and a comprehensive language test was conducted on the day of surgery; 3, 7, and 14 days after surgery. Our follow-up information of was the comprehensive language test from telephone interviews in 3 months after surgery.

Results: Thirty-three patients experienced transient language impairment within 1 week of surgery. Fourteen patients had permanent language impairment. Type II tumors, shorter distance from the tumor to the posterior superior longitudinal fasciculus/arcuate fasciculus, and isocitrate dehydrogenase mutations were risk factors for surgery-related language impairment. Regarding the presence or absence of permanent surgery-related language impairments, the cut-off distance between the tumor and posterior superior longitudinal fasciculus/arcuate fasciculus was 2.75 mm.

Conclusions: According to our classification, patients with type II tumors had the worst language prognosis and longest recovery time. Our classification, based on tumor location, can reliably predict postoperative language status and may be used to guide tumor resection.
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http://dx.doi.org/10.1007/s11060-021-03858-9DOI Listing
November 2021

Optimal dose of perineural dexmedetomidine to prolong analgesia after brachial plexus blockade: a systematic review and Meta-analysis of 57 randomized clinical trials.

BMC Anesthesiol 2021 09 28;21(1):233. Epub 2021 Sep 28.

Department of Anesthesiology, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.

Background And Objectives: Peripheral injection of dexmedetomidine (DEX) has been widely used in regional anesthesia to prolong the duration of analgesia. However, the optimal perineural dose of DEX is still uncertain. It is important to elucidate this characteristic because DEX may cause dose-dependent complications. The aim of this meta-analysis was to determine the optimal dose of perineural DEX for prolonged analgesia after brachial plexus block (BPB) in adult patients undergoing upper limb surgery.

Method: A search strategy was created to identify suitable randomized clinical trials (RCTs) in Embase, PubMed and The Cochrane Library from inception date to Jan, 2021. All adult patients undergoing upper limb surgery under BPB were eligible. The RCTs comparing DEX as an adjuvant to local anesthetic (LA) with LA alone for BPB were included. The primary outcome was duration of analgesia for perineural DEX. Secondary outcomes included visual analog scale (VAS) in 12 and 24 h, consumption of analgesics in 24 h, and adverse events.

Results: Fifty-seven RCTs, including 3332 patients, were identified. The subgroup analyses and regression analyses revealed that perineural DEX dose of 30-50 μg is an appropriate dosage. With short-/intermediate-acting LAs, the mean difference (95% confidence interval [CI]) of analgesia duration with less than and more than 60 μg doses was 220.31 (153.13-287.48) minutes and 68.01 (36.37-99.66) minutes, respectively. With long-acting LAs, the mean differences (95% CI) with less than and more than 60 μg doses were 332.45 (288.43-376.48) minutes and 284.85 (220.31-349.39) minutes.

Conclusion: 30-50 μg DEX as adjuvant can provides a longer analgesic time compared to LA alone and it did not increase the risk of bradycardia and hypotension.
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http://dx.doi.org/10.1186/s12871-021-01452-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477554PMC
September 2021

Intra-operative mapping and language protection in glioma.

Chin Med J (Engl) 2021 Sep 22;134(20):2398-2402. Epub 2021 Sep 22.

Beijing Neurosurgical Institute, Capital Medical University, Beijing 100070, China.

Abstract: The demand for acquiring different languages has increased with increasing globalization. However, knowledge of the modification of the new language in the neural language network remains insufficient. Although many details of language function have been detected based on the awake intra-operative mapping results, the language neural network of the bilingual or multilingual remains unclear, which raises difficulties in clinical practice to preserve patients' full language ability in neurosurgery. In this review, we present a summary of the current findings regarding the structure of the language network and its evolution as the number of acquired languages increased in glioma patients. We then discuss a new insight into the awake intra-operative mapping protocol to reduce surgical risks during the preservation of language function in multilingual patients with glioma.
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http://dx.doi.org/10.1097/CM9.0000000000001751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654440PMC
September 2021

Moderating effect of work-related social support on the relationship between role conflicts and job satisfaction among female nurses pursuing a further degree in China.

Int J Nurs Pract 2021 Sep 12:e13014. Epub 2021 Sep 12.

Medicine College of Lishui University, Lishui, China.

Purpose: This study aimed to determine the moderating effect of work-related social support on the relationship between work-family-school role conflicts and job satisfaction among female registered nurses pursuing further degrees in China.

Methods: The study had a cross-sectional correlational design. Convenience sampling was applied to recruit participants from eight hospitals in China from January to August 2017. SPSS software and the PROCESS macro were used to conduct the data analyses in the present study.

Results: A total of 320 nurses were included in the present study. Work-family-school role conflicts were negatively correlated with job satisfaction (r = -0.21, P < 0.001). Work-related social support was a significant moderator (interaction effect: β = 0.22, P = 0.039) on the relationship between work-family-school role conflicts and job satisfaction.

Conclusions: The study revealed that when nurses perceived that they received a low or moderate level of social support in the workplace that strengthened the relationship between work-family-school role conflicts and job satisfaction. Hospital administrators should implement school- and family-friendly policies that increase the social support provided to nurses pursuing further degrees to balance their responsibilities of work, family and school and to improve their job satisfaction.
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http://dx.doi.org/10.1111/ijn.13014DOI Listing
September 2021

Pretreatment with metformin prevents microcystin-LR-induced tau hyperphosphorylation via mTOR-dependent PP2A and GSK-3β activation.

Environ Toxicol 2021 Dec 25;36(12):2414-2425. Epub 2021 Aug 25.

School of Pharmacy, Nantong University, Nantong, China.

Microcystin-leucine-arginine (MC-LR) is a toxin secreted by freshwater cyanobacteria that is considered a potential environmental risk factor for Alzheimer's disease (AD). A previous study indicated that tau protein hyperphosphorylation via protein phosphatase 2A (PP2A) and GSK-3β inhibition was the mechanism by which MC-LR induces neurotoxicity; however, how MC-LR-induced neurotoxicity can be effectively prevented remains unclear. In this study, the reversal effect of metformin on MC-LR-induced neurotoxicity was investigated. The results showed that metformin effectively prevented tau hyperphosphorylation at Ser202 caused by MC-LR through PP2A and GSK-3b activity. The effect of metformin on PP2A activity was dependent on the inhibition of mTOR in MC-LR-treated SH-SY5Y cells. Metformin prevented spatial memory deficits in rats caused by intrahippocampal MC-LR administration. In sum, the results suggested that metformin can ameliorate the MC-LR-induced AD-like phenotype by preventing tau phosphorylation at Ser202, which was mainly mediated by mTOR-dependent PP2A and GSK-3β activation.
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http://dx.doi.org/10.1002/tox.23354DOI Listing
December 2021

Chloroplast Phylogenomic Analyses Resolve Multiple Origins of the Species (Poaceae: Triticeae) Independent Polyploidization Events.

Front Plant Sci 2021 6;12:682040. Epub 2021 Aug 6.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

is a group of allohexaploid species that arose from two hybridization events followed by genome doubling of three ancestral diploid species with different genomes St, Y, and P in the Triticeae. Estimating the phylogenetic relationship in resolution of the maternal lineages has been difficult, owing to the extremely low rate of sequence divergence. Here, phylogenetic reconstructions based on the plastome sequences were used to explore the role of maternal progenitors in the establishment of polyploid species. The plastome sequences of 11 species were analyzed together with 12 tetraploid species (PP, StP, and StY) and 33 diploid taxa representing 20 basic genomes in the Triticeae. Phylogenomic analysis and genetic divergence patterns suggested that (1) is closely related to , , , , , and ; (2) both the StY genome tetraploids and the PP genome tetraploids served as the maternal donors during the speciation of species; (3) the different species derived their StY genome from different species. Multiple origins of species independent polyploidization events have occurred in the genus , resulting in a maternal haplotype polymorphism. This helps explain the rich diversity and wide adaptation of polyploid species in the genus .
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http://dx.doi.org/10.3389/fpls.2021.682040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377392PMC
August 2021
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