Publications by authors named "Xing Chen"

1,065 Publications

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Insights into the photocatalytic peroxymonosulfate activation over defective boron-doped carbon nitride for efficient pollutants degradation.

J Hazard Mater 2021 Jun 5;418:126338. Epub 2021 Jun 5.

School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China; Key Laboratory of Nanominerals and Pollution Control of Higher Education Institutes, Hefei University of Technology, Hefei 230009, China.

The metal-free graphitic carbon nitride is a promising photocatalyst for peroxymonosulfate (PMS) activation towards water decontamination, but bearing low efficiency due to its electronic structure and surface chemistry. Herein, the non-metallic element boron was adopted for catalyst development. The boron dopants and defects were simultaneously introduced by potassium borohydride, resulting in an excellent activity towards PMS activation. The dominant reactive oxygen species was singlet oxygen, which was determined to originate from PMS activation over photo-induced holes initiated by an electron transfer process. Calculations based on density functional theory revealed that at excited states, due to the dopants and defects, the electron-hole distribution was altered from an even population to a significant separation, which was beneficial for photocatalytic performance. Besides, the engineered electronic structure weakened the catalyst resistance to charge transfer, enabling easier electron transfer between the catalyst and the PMS. Moreover, the strengthened and enlarged positive electrostatic potential areas on heptazine rings oriented the electron transfer process from the negatively charged PMS to the catalyst, facilitating the generation of singlet oxygen. These findings provide underlying mechanism insights into the contribution of dopants and defects to catalytic performance on persulfate-based photocatalytic water treatment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126338DOI Listing
June 2021

Pan-ancestry exome-wide association analyses of COVID-19 outcomes in 586,157 individuals.

Am J Hum Genet 2021 Jun 3. Epub 2021 Jun 3.

Centre for Genomics Research, Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, Cambridge CB2 0AA, UK.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19), a respiratory illness that can result in hospitalization or death. We used exome sequence data to investigate associations between rare genetic variants and seven COVID-19 outcomes in 586,157 individuals, including 20,952 with COVID-19. After accounting for multiple testing, we did not identify any clear associations with rare variants either exome wide or when specifically focusing on (1) 13 interferon pathway genes in which rare deleterious variants have been reported in individuals with severe COVID-19, (2) 281 genes located in susceptibility loci identified by the COVID-19 Host Genetics Initiative, or (3) 32 additional genes of immunologic relevance and/or therapeutic potential. Our analyses indicate there are no significant associations with rare protein-coding variants with detectable effect sizes at our current sample sizes. Analyses will be updated as additional data become available, and results are publicly available through the Regeneron Genetics Center COVID-19 Results Browser.
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http://dx.doi.org/10.1016/j.ajhg.2021.05.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173480PMC
June 2021

Highly efficient electrocatalytic hydrogen evolution promoted by O-Mo-C interfaces of ultrafine β-MoC nanostructures.

Chem Sci 2020 Mar 12;11(13):3523-3530. Epub 2020 Mar 12.

Department of Chemistry, University of South Florida 4202 E. Fowler Avenue Tampa Florida 33620 USA

Optimizing interfacial contacts and thus electron transfer phenomena in heterogeneous electrocatalysts is an effective approach for enhancing electrocatalytic performance. Herein, we successfully synthesized ultrafine β-MoC nanoparticles confined within hollow capsules of nitrogen-doped porous carbon (β[email protected]) and found that the surface layer of molybdenum atoms was further oxidized to a single Mo-O surface layer, thus producing intimate O-Mo-C interfaces. An arsenal of complementary technologies, including XPS, atomic-resolution HAADF-STEM, and XAS analysis clearly reveals the existence of O-Mo-C interfaces for these surface-engineered ultrafine nanostructures. The β[email protected] electrocatalyst exhibited excellent electrocatalytic activity for the hydrogen evolution reaction (HER) in water. Theoretical studies indicate that the highly accessible ultrathin O-Mo-C interfaces serving as the active sites are crucial to the HER performance and underpinned the outstanding electrocatalytic performance of β[email protected] This proof-of-concept study opens a new avenue for the fabrication of highly efficient catalysts for HER and other applications, whilst further demonstrating the importance of exposed interfaces and interfacial contacts in efficient electrocatalysis.
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http://dx.doi.org/10.1039/d0sc00427hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152622PMC
March 2020

Loss of immobilized water and intense protein aggregation responsible for quality deterioration of ready to eat firm tofu.

J Texture Stud 2021 Jun 7. Epub 2021 Jun 7.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

The influence of high-temperature treatment (100-120°C, 15 min) on the texture, color, and water-holding capacity of tofu gels was investigated. As the temperature increasing, the hardness and chewiness as well as the values of redness a and yellowness b increased gradually, while the water content and the lightness L value reduced progressively, and these variations were more pronounced at 115 or 120°C. Low field nuclear magnetic resonance showed that the loss of T water led to the decrease of the water content. Scanning electron microscope revealed that the micropore in gels decreased after heating, and almost entirely disappeared at 120°C. Further analysis by SDS-PAGE indicated the soy protein aggregation formed via disulfide linkage was observed in the thermal treated tofu gels, and nondisulfide linkage might also be occurred as temperature reached 110°C or higher. The quality deterioration may be attributed to immobilized water loss combined with the protein aggregation.
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http://dx.doi.org/10.1111/jtxs.12614DOI Listing
June 2021

Protein deamidation to produce processable ingredients and engineered colloids for emerging food applications.

Compr Rev Food Sci Food Saf 2021 May 30. Epub 2021 May 30.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.

With the ever-increasing demands for functional and sustainable foods from the general public, there is currently a paradigm shift in the food industry toward the production of novel protein-based diet. Food scientists are therefore motivated to search for natural protein sources and innovative technologies to modify their chemical structure for desirable functionality and thus utilization. Deamidation is a viable, efficient, and attractive approach for modifying proteins owing to its ease of operating, specificity, and cost-effective processes. Over the past three decades, the knowledge of protein deamidation for food applications has evolved drastically, including the development of novel approaches for deamidation, such as protein-glutaminase and ion exchange resin, and their practices in new protein substrate. Thanks to deamidation, enhanced functionalities of food proteins from cereals, legumes, milk, oil seeds and others, and thereby their processabilities as food ingredients have been achieved. Moreover, deamidated proteins have been used to fabricate engineered food colloids, including self-assembled protein particles, protein-metallic complexes, and protein-carbohydrate complexes, which have demonstrated tailored physicochemical properties to modulate oral perception, improve gastrointestinal digestion and bioavailability, and protect and/or deliver bioactive nutrients. Novel bioactivity, altered digestibility, and varied allergenicity of deamidated proteins are increasingly recognized. Therefore, deamidated proteins with novel techno-functional and biological properties hold both promise and challenges for future food applications, and a comprehensive review on this area is critically needed to update our knowledge and provide a better understanding on the protein deamidation and its emerging applications.
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http://dx.doi.org/10.1111/1541-4337.12759DOI Listing
May 2021

Substance use and psychological distress among school-going adolescents in 41 low-income and middle-income countries.

J Affect Disord 2021 Aug 23;291:254-260. Epub 2021 May 23.

School of Public Health, Medical College of Soochow University, Suzhou, China. Electronic address:

Background: Substance use remains a public health problem worldwide, and it is particularly concerning among adolescents because of its harmful effects. There is a need to better understand the factors associated with adolescents' substance use to inform policies for effective prevention.

Methods: We used data from the Global School-based Student Health Surveys. Data were collected by anonymous self-report questionnaires. This study is based on three questions assessing substance use and four questions assessing psychological distress. Logistic regression models were used to analyse associations between substance use and psychological distress. We calculated the pooled overall and regional estimates by random-effects meta-analysis.

Results: We included 126,763 adolescents (boys, 44.7%; girls, 55.3%). Substance users tended to be older, male, physically attacked, in physical fighting, bullied; to experience psychological distress; and to have a higher socio-economic status. Alcohol use was consistently associated with medium and high psychological distress. In terms of four aspects of psychological distress, only suicidal ideation was consistently associated with the substance use components and all specific combinations of substance use. Additionally, the prevalence of alcohol use was higher than that of cigarette use and drug use. Region-wise analysis showed that substance use was associated with psychological distress in the African region, American region, South-East region and Western Pacific region.

Limitations: The cross-sectional data could not infer causality.

Conclusions: This study indicated that high levels of substance use were significantly associated with an increased likelihood of psychological distress among adolescents. In addition, age also had an effect on substance use.
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http://dx.doi.org/10.1016/j.jad.2021.05.024DOI Listing
August 2021

Observation of the characteristics of the natural course of Bietti crystalline dystrophy by fundus fluorescein angiography.

BMC Ophthalmol 2021 May 28;21(1):239. Epub 2021 May 28.

Beijing Institute of Ophthalmology, Beijing Ophthalmology and Visual Science Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, 17 Hougou Lane, Chongnei Street, 100005, Beijing, People's Republic of China.

Background: Bietti crystalline dystrophy (BCD) is an autosomal recessive genetic disorder that causes progressive vision loss. Here, 12 patients were followed up for 1-5 years with fundus fluorescein angiography (FFA) to observe BCD disease progression.

Methods: FFA images were collected for 12 patients with BCD who visited our clinic twice or more over a 5-year period. Peripheral venous blood was collected to identify the pathogenic gene related to the clinical phenotype.

Results: We observed two types in FFA images of patients with BCD. Type 1 showed retinal pigment epithelium (RPE) atrophy in the macular area, followed by choriocapillaris atrophy and the subsequent appearance of RPE atrophy appeared at the peripheral retina. Type 2 showed RPE atrophy at the posterior pole and peripheral retina, followed by choriocapillaris atrophy around the macula and along the superior and inferior vascular arcades and the nasal side of the optic disc. The posterior and peripheral lesions of both type 1 and type 2 BCD subsequently extended to the mid-periphery; finally, all the RPEs and choriocapillaris atrophied, exposing the choroid great vessels, but type 2 macular RPE atrophy could last longer.

Conclusions: The characterization of two different types of BCD development provides a better understanding of the phenotype and the progression of the disease for a precise prognosis and prediction of pathogenesis.
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http://dx.doi.org/10.1186/s12886-021-01999-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161580PMC
May 2021

Prognostic significance of low TSH concentration in patients with COVID-19 presenting with non-thyroidal illness syndrome.

BMC Endocr Disord 2021 May 27;21(1):111. Epub 2021 May 27.

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Low free triiodothyronine (FT3) levels are related to a poor prognosis deterioration in patients with COVID-19 presenting with non-thyroidal illness syndrome (NTI). This study was designed to explore whether free thyroxin (FT4) or thyroid stimulating hormone (TSH) levels affected the mortality of patients with COVID-19 presenting with NTI.

Methods: Patients with COVID-19 complicated with NTI who were treated at our hospital were included in this retrospective study. Patients were divided into low TSH and normal TSH groups, as well as low and normal-high FT4 group, according to the reference range of TSH or FT4 levels. The 90-day mortality and critical illness rates were compared among patients with low and normal TSH levels, as well as among patients with low FT4 levels and normal-high FT4 levels; in addition, differences in demographic and laboratory data were compared. A Kaplan-Meier analysis and Cox proportional hazards models were used to assess the associations of TSH and FT4 levels with mortality.

Results: One hundred fifty patients with low FT3 levels and without a history of thyroid disease were included, 68% of whom had normal FT4 and TSH levels. Critical illness rates (74.07% VS 37.40%, P = 0.001) and mortality rates (51.85% VS 22.76%, P = 0.002) were significantly higher in the low TSH group than in the normal TSH group. Although no significant difference in the critical illness rate was found (P = 0.296), the mortality rate was significantly higher in the low FT4 group (P = 0.038). Low TSH levels were independently related to 90-day mortality (hazard ratio = 2.78, 95% CI:1.42-5.552, P = 0.003).

Conclusions: Low FT4 and TSH concentrations were associated with mortality in patients with COVID-19 presenting with NTI; moreover, low TSH levels were an independent risk factor for mortality in these patients.
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http://dx.doi.org/10.1186/s12902-021-00766-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159017PMC
May 2021

Value of neutrophil-to-lymphocyte ratio as a marker of renal damage in patients with H-type hypertension.

Biomark Med 2021 Jun 27;15(9):637-646. Epub 2021 May 27.

Hunan University of Traditional Chinese Medicine, Changsha, Hunan, China.

To explore the relationship between the neutrophil-to-lymphocyte ratio (NLR) and renal damage in patients with H-type hypertension. A total of 618 patients between 2017 and 2019 were analyzed retrospectively. NLR was significantly correlated with renal damage in hypertension patients. Appropriate cut-off value for NLR (2.247) was determined by receiver operating characteristic curve; linear regression analysis showed that NLR and estimated glomerular filtration rate, blood urea nitrogen/creatinine has a significant negative correlation in H-type hypertension group (p < 0.05); logistic regression analysis showed that the risk of renal damage increased by 10% for each 1 umol/l increase of homocysteine, and 51% for each 1.0 increase of NLR in H-type hypertension patients. NLR worth popularizing in prediction of renal damage in patients with H-type hypertension.
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http://dx.doi.org/10.2217/bmm-2020-0638DOI Listing
June 2021

Novel pyridine-containing sultones: Structure-activity relationship and biological evaluation as selective AChE inhibitors for the treatment of Alzheimer's disease.

ChemMedChem 2021 May 25. Epub 2021 May 25.

Wuhan University of Technology, School of Chemistry, CHINA.

Novel pyridine-containing sultones were synthesized and evaluated for their ChE inhibitory activity. Most of compounds showed selective AChE inhibitory activity. The structure-activity relationship (SAR) showed: (i) fused pyridine-containing sultones increased the AChE inhibition, series B > series A ; (ii) series B with halo-phenyl had better activity. Compound B4 was identified as a selective AChE inhibitor (IC 50 = 8.93 μM), which was nicely fallen into Tc AChE via hydrogen interactions between δ-pyridylsultone scaffold with Asp72, Ser122, Phe288, Phe290 and Trp84. Compound B4 showed reversible and non-competitive ( K i = 7.67 µM) AChE inhibition, nontoxicity and remarkable neuro-protective activity. In vivo studies confirmed that compound B4 significantly ameliorates performances of scopolamine-treated C57BL/6J mice, suggesting a significant benefit of AChE inhibition for a disease-modifying treatment of AD.
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http://dx.doi.org/10.1002/cmdc.202100272DOI Listing
May 2021

Therapeutic effect of various ginsenosides on rheumatoid arthritis.

BMC Complement Med Ther 2021 May 25;21(1):149. Epub 2021 May 25.

Department of Pharmaceutics, School of Pharmacy, Fudan University & Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai, 201203, China.

Background: Rheumatoid arthritis (RA) is an autoimmune disease which causes disability and threatens the health of humans. Therefore, it is of great significance to seek novel effective drugs for RA. It has been reported that various ginsenoside monomers are able to treat RA. However, it is still unclear which ginsenoside is the most effective and has the potential to be developed into an anti-RA drug.

Methods: The ginsenosides, including Rg1, Rg3, Rg5, Rb1, Rh2 and CK, were evaluated and compared for their therapeutic effect on RA. In in vitro cell studies, methotrexate (MTX) and 0.05% dimethyl sulfoxide (DMSO) was set as a positive control group and a negative control group, respectively. LPS-induced RAW264.7 cells and TNF-α-induced HUVEC cells were cultured with MTX, DMSO and six ginsenosides, respectively. Cell proliferation was analyzed by MTT assay and cell apoptosis was carried out by flow cytometry. CIA mice model was developed to evaluate the therapeutic efficacy of ginsenosides. The analysis of histology, immunohistochemistry, flow cytometry and cytokine detections of the joint tissues were performed to elucidate the action mechanisms of ginsenosides.

Results: All six ginsenosides showed good therapeutic effect on acute arthritis compared with the negative control group, Ginsenoside CK provided the most effective treatment ability. It could significantly inhibit the proliferation and promote the apoptosis of RAW 264.7 and HUVEC cells, and substantially reduce the swelling, redness, functional impairment of joints and the pathological changes of CIA mice. Meanwhile, CK could increase CD8 + T cell to down-regulate the immune response, decrease the number of activated CD4 + T cell and proinflammatory M1-macrophages, thus resulting in the inhibition of the secretion of proinflammatory cytokine such as TNF-α and IL-6.

Conclusion: Ginsenoside CK was proved to be a most potential candidate among the tested ginsenosides for the treatment of RA, with a strong anti-inflammation and immune modulating capabilities.
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http://dx.doi.org/10.1186/s12906-021-03302-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145820PMC
May 2021

Evaluating the genesis and dominant processes of groundwater salinization by using hydrochemistry and multiple isotopes in a mining city.

Environ Pollut 2021 Aug 18;283:117381. Epub 2021 May 18.

School of Resources and Environmental Engineering, Anhui University, Anhui Province Engineering Laboratory for Mine Ecological Remediation, Hefei, 230601, Anhui, China.

The increasing salinization of groundwater renders it challenging to maintain the water quality. Moreover, knowledge regarding the characteristics and mechanism of groundwater salinization in mining areas remains limited. This study represents the first attempt of combining the hydrochemical, isotope (δD, δO, δCl, and Sr/Sr) and multivariate statistical analysis methods to explore the origin, control, and influence of fluoride enrichment in mining cities. The TDS content of groundwater ranged from 275.9 mg/L to 2452.0 mg/L, and 54% of the groundwater samples were classified as class IV water according to China's groundwater quality standards (GB/T 14848-2017), indicating a decline in the water quality of the study area. The results of the groundwater ion ratio and isotope discrimination analysis showed that dissolution and evaporation involving water-rock interactions and halite were the main driving processes for groundwater salinization in the study area. In addition to the hydrogeological and climatic conditions, mine drainage inputs exacerbated the increasing salinity of the groundwater in local areas. The mineral dissolution, cation exchange, and evaporation promoted the F enrichment, while excessive evaporation and salinity inhibited the F enrichment. Gangue accumulation and infiltration likely led to considerable F enrichment in individual groundwater regions. Extensive changes in the groundwater salinity indicated differences in the geochemical processes that controlled the groundwater salinization. Given the particularity of the study area, the enrichment of salinization and fluoride triggered by mining activities cannot be ignored.
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http://dx.doi.org/10.1016/j.envpol.2021.117381DOI Listing
August 2021

Real time detection of 3-nitrotyrosine using smartphone-based electrochemiluminescence.

Biosens Bioelectron 2021 Sep 7;187:113284. Epub 2021 May 7.

Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education of China, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, 310027, Zhejiang, China. Electronic address:

As an oxidase stress biomarker, 3-nitrotyrosine is closely associated with many cardiovascular diseases. Thus, early diagnosis and real time detection of 3-nitrotyrosine at bedside are highly important. Herein, we developed a handheld electrochemiluminescence (ECL) analysis device, which integrates printed circuit board (PCB) for electrical stimulation and smartphone for optical signals readout. Fast and accurate determination of 3-nitrotyrosine was achieved with Antibody/Ru(dcpy)@AuNPs/MoS modified Au electrode (Ab/[email protected]/MoS) for ECL analysis. The linear range of 3-nitrotyrosine detection was from 10 mol/L to 10 mol/L with a detection limit of 8.4 × 10 mol/L. In addition, an Android application was developed to realize real time analysis of ECL emissions and results readout for detection. To confirm the usage of the device, spiked serum with different concentrations was tested and the results indicated the practical reliability and stability of this device. The operating procedure for ECL analysis in this device is extremely easy and electrical stimulation was adjustable from 0 V to 5 V for general ECL systems. Thus, we believe this handheld device for ECL analysis has extensive prospects for application in Point-of-care testing and health caring.
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http://dx.doi.org/10.1016/j.bios.2021.113284DOI Listing
September 2021

Deep-belief network for predicting potential miRNA-disease associations.

Brief Bioinform 2021 May;22(3)

School of Information and Control Engineering, China University of Mining and Technology.

MicroRNA (miRNA) plays an important role in the occurrence, development, diagnosis and treatment of diseases. More and more researchers begin to pay attention to the relationship between miRNA and disease. Compared with traditional biological experiments, computational method of integrating heterogeneous biological data to predict potential associations can effectively save time and cost. Considering the limitations of the previous computational models, we developed the model of deep-belief network for miRNA-disease association prediction (DBNMDA). We constructed feature vectors to pre-train restricted Boltzmann machines for all miRNA-disease pairs and applied positive samples and the same number of selected negative samples to fine-tune DBN to obtain the final predicted scores. Compared with the previous supervised models that only use pairs with known label for training, DBNMDA innovatively utilizes the information of all miRNA-disease pairs during the pre-training process. This step could reduce the impact of too few known associations on prediction accuracy to some extent. DBNMDA achieves the AUC of 0.9104 based on global leave-one-out cross validation (LOOCV), the AUC of 0.8232 based on local LOOCV and the average AUC of 0.9048 ± 0.0026 based on 5-fold cross validation. These AUCs are better than other previous models. In addition, three different types of case studies for three diseases were implemented to demonstrate the accuracy of DBNMDA. As a result, 84% (breast neoplasms), 100% (lung neoplasms) and 88% (esophageal neoplasms) of the top 50 predicted miRNAs were verified by recent literature. Therefore, we could conclude that DBNMDA is an effective method to predict potential miRNA-disease associations.
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http://dx.doi.org/10.1093/bib/bbaa186DOI Listing
May 2021

Metabolic profiles of in biofilm and planktonic states and investigation of its intestinal modulation and immunoregulation in dogs.

Food Funct 2021 Jun;12(12):5317-5332

School of food science and bioengineering, Xihua University, Hongguang Street, Pidu District, Chengdu, 610039, China.

The use of probiotics has recently become a considerably promising research area. The most advanced fourth-generation probiotics involve beneficial bacteria enclosed in biofilms. However, differences in the effects of probiotics in biofilm and those in planktonic states are, as yet, unclear. In this study, it was ascertained that the biofilm mode of Lactobacillus paraplantarum L-ZS9 had a comparatively higher density and stronger resistance. Untargeted metabolomics analysis suggested a significant distinction between planktonic and biofilm cells, with amino acids and carbohydrate metabolism both more active in the biofilm mode. Furthermore, the in vivo experiment showed that the biofilm strain displayed better immunomodulation activity, which could increase the relative abundance of Lactobacillus in the intestinal microbiota of dogs. The relative abundance of intestinal microbiota participating in carbohydrate metabolism was higher in the biofilm probiotic-treated dogs. Correlation analysis between L-ZS9-producing metabolites, dog intestinal microbiome diversity and dog blood immune indexes (sIgA or IgG) revealed the interaction between these three components, which might explain the mechanisms by which biofilm L-ZS9 regulated the intestinal microbiome and immunity activity of the host, through the production of various metabolites. Findings of this study will, thus, enhance understanding of the beneficial effects of biofilm probiotics, as well as provide references for further investigation.
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http://dx.doi.org/10.1039/d1fo00905bDOI Listing
June 2021

An improved cosmetic suture method without stitch removal for facial skin.

J Plast Reconstr Aesthet Surg 2021 May 6. Epub 2021 May 6.

Department of Cosmetology and Dermatology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China. Electronic address:

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http://dx.doi.org/10.1016/j.bjps.2021.03.132DOI Listing
May 2021

Study of the micromorphology and health risks of arsenic in copper smelting slag tailings for safe resource utilization.

Ecotoxicol Environ Saf 2021 Aug 12;219:112321. Epub 2021 May 12.

School of Resources and Environmental Engineering, Anhui Province Engineering Laboratory for Mine Ecological Remediation, Anhui University, Hefei 230601, PR China. Electronic address:

Slag tailings are produced by "cooling-grinding-ball milling-flotation" and other processes of slag, while slag is produced by the flash smelting of the original ore. The utilization and environmental hazards of arsenic in slag tailings have become a focus of attention. This study on slag tailings reveals the presence of arsenic in copper smelting tailings from the mineralogy and leaching perspectives, and the noncarcinogenic and carcinogenic risks of arsenic to the human body were assessed by using the USEPA health risk model. The surface particles of the slag tailings were unevenly dispersed, and the mineral crystals were relatively complete. A small amount of secondary minerals had grown on the mineral surface. Most of the fine particles adhered to the surface of the main mineral to form inclusions. The mineral composition of the slag tailings was dominated by maghemite (FeO) and fayalite (FeSiO), and the arsenic-bearing minerals were unevenly distributed, where As (Ⅴ) fine particles were embedded in maghemite, amorphous phase and fayalite. There was a large amount of residual arsenic in the slag tailing particles, and the leaching content of arsenic in the toxicity leaching procedure was always lower than the limit of 5 mg/L. The health risk to the exposed population was evaluated by the USEPA health risk model. Since the exposed population in the industrial land is mainly adults, it is determined that the tailings will not cause harm to children's health. In this evaluation, the exposure duration (length of service of the workers) of 30 years, exposure frequency of 314 d/y and body weight of 60 kg (average weight of the workers) were taken as the parameters of three exposure pathways: hand-oral ingestion, respiratory system inhalation and skin contact. Therefore, longer activity time of the workers in the tailing workshop corresponds to a higher HI (hazard index). Although the arsenic in the slag tailings had a certain degree of bioavailability, it was not sufficient to adversely affect human health.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112321DOI Listing
August 2021

In vivo monitoring of volatile metabolic trajectories enables rapid diagnosis of influenza A infection.

Chem Commun (Camb) 2021 May 15;57(39):4791-4794. Epub 2021 Apr 15.

Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou 510632, China.

We report that influenza A virus infection induces changes in odor traits that could be captured by real-time high-resolution mass spectrometry in a living mouse model. The most striking changes in the volatile metabolites may be associated mostly to glyoxylate/dicarboxylate metabolism.
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http://dx.doi.org/10.1039/d1cc01061aDOI Listing
May 2021

Circular RNA UBAP2 promotes the proliferation of prostate cancer cells via the miR-1244/MAP3K2 axis.

Oncol Lett 2021 Jun 21;21(6):486. Epub 2021 Apr 21.

Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.

Prostate cancer (PCa) is a common male malignant disease with a high incidence, which can seriously affect the quality of life of patients. The survival rate of patients with PCa has improved to 98.6%; however, new insights for the molecular mechanism are still urgently required. Circular RNA (circ)UBAP2 is a tumor-associated circRNA that has been demonstrated to promote the progression of various types of cancer. CircUBAP2 has been demonstrated to be significantly upregulated in PCa, but its role in the progression of PCa remains unclear. The present study aimed to provide an improved understanding of the regulatory mechanism of circUBAP2 in PCa. circUBAP2 expression was identified to be upregulated in four PCa cell lines and clinical tissues by using reverse transcription-quantitative PCR analysis. Binding sites analysis and luciferase reporter gene assay indicated that the microRNA(miR)-1244/MAP3K2 axis was the target of circUBAP2. Gain-of-function assays revealed that circUBAP2 promoted the proliferation of PCa cells by sponging miR-1244 and promoting the MAP3K2 axis. The present findings may be essential for providing new strategies in the diagnosis and targeted therapy of PCa.
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http://dx.doi.org/10.3892/ol.2021.12747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100954PMC
June 2021

A case report of right upper lung adenosquamous carcinoma resection following neoadjuvant targeted therapy.

Ann Palliat Med 2021 Apr;10(4):4987-4993

Department of Thoracic Surgery, Fuzhou Pulmonary Hospital of Fujian, Fuzhou, China.

The patient was a middle-aged male smoker who had space-occupying lesions in the right upper lung, Positron emission tomography-computed tomography (PET-CT) suggested right upper lung cancer with multiple mediastinal lymph node metastases. Endobronchial ultrasound-guided transbronchial lung biopsy (EBUS-GS-TBLB) performed in the bronchus of the right apical segment confirmed the lesion as squamous cell carcinoma. Neoadjuvant therapy was planned. Because genetic testing revealed the epidermal growth factor receptor (EGFR) L858R mutation, the possibility of adenosquamous carcinoma was considered. The patient was clinically diagnosed with right upper lung squamous cell carcinoma c-T3N2M0, stage IIIB, and PS 1 point. Dacomitinib was selected for the targeted therapy. Eight weeks after the initiation of treatment, efficacy was assessed as a partial response (PR), suggesting a possibility of R0 resection. After sufficient communication with the patient and his family members, on July 1, 2020, thoracoscopic radical resection of the right upper lung cancer was performed under general anesthesia. Postoperative pathology confirmed adenosquamous carcinoma, of which 80% were adenocarcinoma and 20% were squamous cell carcinoma; no tumor thrombus was seen in the interstitial vessels; and the tumor did not invade the visceral pleura. There was no cancerous infiltration in the bronchial stump of the right upper lung. The following were the results for lymph nodes submitted for examination: L2 (0/1), L4 (0/4), L7 (0/3), L8 (0/7), L10 (0/1), L11 (0/2) and L11 (frozen 0/1) showed no cancer metastasis. The postoperative diagnosis was as follows: adenosquamous carcinoma of the right upper lung p-T2N0M0, stage Ib. After surgery, 4 cycles of the GC (gemcitabine + carboplatin) chemotherapy regimen were given, with continued targeted therapy recommended for 2 years. The patient has been followed-up and is in good condition.
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http://dx.doi.org/10.21037/apm-21-868DOI Listing
April 2021

MiR-133a delivery to osteoblasts ameliorates mechanical unloading-triggered osteopenia progression in vitro and in vivo.

Int Immunopharmacol 2021 May 4;97:107613. Epub 2021 May 4.

Department of Orthopaedics, Changxing People's Hospital, Huzhou, Zhejiang 313100, China.

Mechanical unloading-induced bone loss is a clinical challenge, and deep understanding for this disease is necessary for developing novel and effective therapies. MicroRNAs (miRNAs) are small non-coding RNAs, and involved in bone remodeling. In the study, we attempted to explore the potential of miR-133a in regulating osteoblast activation and its anti-osteopenia function both in vitro and in vivo. Our in vitro studies at first showed that miR-133a could significantly promote the expression of osteocalcin (OCN), Collagen I, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2) and osterix (Osx), promoting the activation and mineralization of osteoblasts. Then, hindlimb unloading (HU)-challenged mice were established with or without intravenous injection of agomir-miR-133a using an osteoblast-targeting delivery system. We found that miR-133a in osteoblasts significantly alleviated the bone loss, microstructural, and biomechanical property in mice with mechanical unloading, contributing to osteopenia alleviation. Furthermore, both in vitro and in vivo experiments showed that miR-133a could restrain osteoclastogenesis via tartrate-resistant acid phosphatase (TRAP) staining. In conclusion, our results suggested that miR-133a may be a promising factor in mediating the occurrence and progression of osteopenia caused by mechanical unloading, and thus targeting miR-133a could be considered as an effective therapeutic strategy for the suppression of pathological osteopenia.
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http://dx.doi.org/10.1016/j.intimp.2021.107613DOI Listing
May 2021

Circadian oscillation expression of ornithine carbamoyltransferase and its significance in sleep disturbance.

Biochem Biophys Res Commun 2021 Jun 3;559:217-221. Epub 2021 May 3.

Beijing Institute of Basic Medical Sciences, Beijing, 100850, China; School of Pharmacy, Jiamus University, Jiamusi, 154007, China; Anhui Medical University, 81 Meishan Road, Hefei, 230032, China; College of Life Science, Henan Normal University, 46 Jianshe Road, Xinxiang, 473007, China. Electronic address:

Ornithine transcarbamylases (OTC), a key enzyme in urea cycle, is an important marker for some liver injury or diseases. However, whether OTC could be a sensitive indicator for liver dysfunction under sleep disturbance condition remains unknown. The present study aimed to explore the circadian oscillation expression of OTC and its significance in disturbed sleep condition. Sleep disturbance was conducted by a sleep deprivation (SD) instrument. Our results found that SD for 72h induced abnormal increasing of OTC levels in serum and liver of rats. And, serum OTC concentration and liver OTC expression could return to normal levels after recovery sleep following SD. Moreover, hepatic OTC expression showed circadian oscillation in day and night, characterized with occurrence of a peak between ZT 22 and ZT 2, and a nadir between ZT 14 and ZT 18. Further analysis suggested the existence of ROR response element (RORE) for potential RORɑ binding sites in OTC promoter region, and elevated RORɑ expression in rat livers under sleep disturbance condition. Additionally, oscillation expression of OTC induced by serum shock in HepG2 cells was characterized with a peak occurred between ZT 12 and ZT 16, and RORɑ knockdown at ZT 16 significantly lowered OTC expression. The results together indicate that OTC is closely correlated with circadian clock, and could be a sensitive indicator for sleep disturbance stress.
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http://dx.doi.org/10.1016/j.bbrc.2021.04.100DOI Listing
June 2021

Cabin hospital for COVID-19 patients: Attention should be paid to hospital infection prevention, humanistic care, and privacy protection.

Kaohsiung J Med Sci 2021 May 4. Epub 2021 May 4.

Hospital Administration Office, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, China.

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http://dx.doi.org/10.1002/kjm2.12382DOI Listing
May 2021

The association between short-term exposure to air pollutants and rotavirus infection in Wuhan, China.

J Med Virol 2021 May 3. Epub 2021 May 3.

Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Background: The impact of various meteorological factors on rotavirus (RV) infection has been previously studied; however, few studies have explored the association between short-term exposure to air pollutants and RV infection.

Methods: Daily RV positive cases among children aged 0-6 years were collected from July 2014 to August 2019 in Tongji hospital (Wuhan, China). Daily data on air temperature and air pollutants were obtained from the China Meteorological Network. A distributed lag model to explore the lagged effects of short-term exposure to air pollutants and RV infection was performed. The distribution lag model was used to study the lag effect of short-term exposure to air pollutants and RV infection.

Results: RV infection was negatively correlated with mean air temperature and O concentration. The RV infection risk decreased by 5.2% and 0.47% for every 1℃ increase in average temperature and 1 ug/m increase in O concentration, respectively. Increased PM , SO , and NO concentrations were independent risk factors for an increase in positive rates; their relative risk values were 1.0014 (95% confidence interval [CI], 1.0013-1.0015), 1.0050 (95% CI, 1.0047-1.0053), and 1.0030 (95% CI, 1.0028-1.0032), respectively. The highest RV-positive rates were from January to March and November to December. Additionally, children <18 months of age and boys were more vulnerable to infection.

Conclusions: Air pollutants were important factors impacting the RV-positivity of children in Wuhan. These findings may help develop an early environment-based warning system to prevent and control RV infection.
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http://dx.doi.org/10.1002/jmv.27047DOI Listing
May 2021

Dexmedetomidine protects against burn-induced intestinal barrier injury via the MLCK/p-MLC signalling pathway.

Burns 2021 Feb 9. Epub 2021 Feb 9.

Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin, People's Republic of China; Tianjin Institute of Anesthesiology, Tianjin, People's Republic of China. Electronic address:

Background: Evidence suggests that sedative dexmedetomidine can prevent intestinal dysfunction. However, the specific mechanisms of its protective effects against burn-induced intestinal barrier injury remain unclear. We aimed to explore the possible positive effects of dexmedetomidine on burn-induced intestinal barrier injury and the effects the myosin light chain kinase (MLCK)/phosphorylated myosin light chain (p-MLC) signalling pathway in an experimental model of burn injury.

Methods: In this study, the plasma concentration of fluorescein isothiocyanate-labelled dextran (FITC-dextran) was measured. Histological changes were evaluated using haematoxylin and eosin (HE) staining. Tight junction proteins were evaluated by western blot and immunofluorescence analyses to assess the structural integrity of intestinal tight junctions. The level of inflammation was detected by enzyme-linked immunosorbent assay (ELISA).

Results: The results shows that the increase in intestinal permeability caused by burn injury is accompanied by histological damage to the intestine, decreases in the expression of the tight junction proteins Zonula Occludens-1 (ZO-1) and Occludin, increases in inflammatory cytokine levels and elevation of both MLCK protein expression and MLC phosphorylation. After dexmedetomidine treatment, the burn-induced changes were ameliorated.

Conclusions: In conclusion, dexmedetomidine exerted an anti-inflammatory effect and protected tight junction complexes against burn‑induced intestinal barrier damage by inhibiting the MLCK/p-MLC signalling pathways.
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http://dx.doi.org/10.1016/j.burns.2021.01.017DOI Listing
February 2021

An Empirical Model for the Churning Losses Prediction of Fluid Flow Analysis in Axial Piston Pumps.

Micromachines (Basel) 2021 Apr 3;12(4). Epub 2021 Apr 3.

School of Mechanical Engineering, Yanshan University, Qinhuangdao 066004, China.

The manufacturing development of axial piston pumps usually takes the trend of high speed and miniaturization, and increases power density. Axial piston pumps are usually characterized as high speed to improve the power density; thus, high-speed churning losses caused by the internal rotating components stirring the oil can increase significantly. In order to improve the efficiency, more attention should be given to the churning losses in axial piston pumps, especially in high-speed conditions. Using the method of least-squares curve fitting, this paper establishes a series of formulas based on the churning losses test rig over a wide range of speeds, which enable accurate predictions of churning losses on the cylinder block and pistons. The reduction coefficient of flow resistance of multi-pistons as calculated. The new churning losses formula devoted to the cylinder block and rotating pistons was validated by comparison with experimental evidence in different geometries of axial piston pumps. According to the prediction model of churning losses, some valuable guidance methods are proposed to reduce the energy losses of the axial piston pump, which are the theoretical support for the miniaturization of axial piston pump manufacturing.
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http://dx.doi.org/10.3390/mi12040398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066296PMC
April 2021

Magnetic recyclable heterogeneous catalyst FeO/g-CN for tetracycline hydrochloride degradation via photo-Fenton process under visible light.

Environ Technol 2021 May 8:1-14. Epub 2021 May 8.

Key Laboratory of Nanominerals and Pollution Control of Higher Education Institutes, School of Resources and Environmental Engineering, Hefei University of Technology, Hefei, People's Republic of China.

Antibiotic pollution of water resources is a global problem, and the development of new treatments for destroying antibiotics in water is a priority research. We successfully manufactured recyclable magnetic FeO/g-CN through the electrostatic self-assembly method. Selecting tetracycline (TC) as the target pollutant, using FeO/g-CN and HO developed a heterogeneous optical Fenton system to remove TC under visible light. FeO/g-CN was systematically characterized by SEM, TEM, XRD, FTIR, XPS, DRS, and electrochemical methods. The removal efficiency of 7% FeO/g-CN at pH = 3, HO = 5 mM, and catalyst dosage of 1.0 g/L can reach 99.8%. After magnetic separation, the FeO/g-CN photocatalyst can be recycled five times with minimal efficiency loss. The excellent degradation performance of the prepared catalyst may be attributed to the proper coupling interface between FeO and g-CN which promotes the separation and transfer of photogenerated electrons. Photogenerated electrons can also accelerate the conversion of Fe to Fe, thereby producing more ˙OH. The new FeO/g-CN can be used as a raw material for advanced oxidation of water contaminated by refractory antibiotics.
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http://dx.doi.org/10.1080/09593330.2021.1921052DOI Listing
May 2021

Tea consumption and serum uric acid levels among older adults in three large-scale population-based studies in China.

BMC Geriatr 2021 04 21;21(1):267. Epub 2021 Apr 21.

School of Public Health, Medical College of Soochow University, 199 Ren Ai Road, Suzhou, 215123, China.

Background And Aims: The association between serum uric acid (SUA) and tea consumption has been studied in previous work, and there were arguments among various population group employed as well as different statistical approaches. The aim of this work is to investigate the tea effect on SUA levels among older adults by comparing three large-scale populations with both cross-sectional and longitudinal analyses.

Method: We examined the relationship between intake and SUA levels among older adults using linear regression. All the studies include the parameters SUA levels, tea intake, age, sex, education level, smoking status, alcohol drinking status, body mass index (BMI), and health history (diabetes, hypertension, and fasting plasma glucose). The cross-sectional analyses were conducted with 4579 older adults in the Weitang Geriatric Diseases Study (WGDS, ≥60 years), 2440 in the China Health and Nutrition Survey (CHNS, ≥60 years) and 1236 in the Chinese Longitudinal Healthy Longevity Survey (CLHLS, ≥62 years); and the longitudinal analyses were performed with 3870 (84.5%) in the WGDS and 420 (34.0%) in the CLHLS. Multivariable linear regression analyses were performed in both cross-sectional and longitudinal studies.

Results: Cross-sectional studies showed that tea consumers tended to have higher SUA levels than non-tea consumers in all the three datasets (P < 0.05). However, longitudinal associations of SUA levels with tea consumption had no statistical significance (P>0.05). The results of sex-stratified analyses were consistent with those of the whole datasets.

Conclusions: This work implied that any possible association between tea consumption and SUA levels could be very weak.
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http://dx.doi.org/10.1186/s12877-021-02216-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061055PMC
April 2021

Menstrual patterns and disorders among Chinese women of reproductive age: A cross-sectional study based on mobile application data.

Medicine (Baltimore) 2021 Apr;100(16):e25329

Department of Obstetrics and Gynecology, Peking University Ninth School of Clinical Medicine, Beijing Shijitan Hospital, Capital Medical University.

Abstract: Menstruation is an important indicator of women's health. Identification of abnormal menstrual patterns in adolescence may improve early diagnosis of potential health concerns in adulthood. This study aimed to evaluate menstrual patterns and disorders of Chinese women of reproductive age based on an APP.From December 2015 to January 2016, a cross-sectional study was conducted. We utilized a mobile application (APP) to collect information about participants' age at menarche, length of menstruation, duration of menstruation, amount of menstrual flow, regularity of menstrual cycle, prevalence of abnormal uterine bleeding and dysmenorrhoea.A total of 156,055 women (25,716 from the questionnaire survey and 130,000 from the mobile APP users) participated in the study. The average age of the subjects was 26.32 ± 6.97 years (median age, 25 years). Mean age at menarche was 13.08 ± 1.87 years; average length of menstrual cycle, 30.9 ± 4.28 days (median 30 days); and average duration of menstruation, 5.01 ± 1.13 days (median 5 days). Women with irregular menstrual cycles accounted for 36.41%. Women aged < 18 years and > 30 years were more likely to experience irregular menstrual cycles. The prevalence of secondary amenorrhoea was 4.07%. More than 20% of women reported abnormal menstrual flow. About 20.11% of women had abnormal uterine bleeding, and 77.65% had dysmenorrhoea. A hot compress was the most commonly used approach to ameliorate dysmenorrhoea. Women with low education and low income and those with high education and high income tended to have menstrual problems.A mobile APP as a survey tool has the advantages of large sample size, low cost, and high efficiency. The use of a mobile APP is an emerging approach for collecting big data in the field of health research. The results showed that the prevalence of menstrual disorders among Chinese reproductive women was high. Healthcare providers should educate girls and their caregivers about menstrual physiology, normal menstrual pattern, and reproductive health to prevent long-term diseases.
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http://dx.doi.org/10.1097/MD.0000000000025329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078451PMC
April 2021