Publications by authors named "Xin-Xin Wang"

103 Publications

Pancreatic outflow tract reconstruction after pancreaticoduodenectomy: a meta-analysis of randomized controlled trials.

World J Surg Oncol 2021 Jul 6;19(1):203. Epub 2021 Jul 6.

Department of General Surgery, Gansu Provincial Hospital, No. 204 Donggang West Road, Lanzhou, 730000, Gansu Province, China.

Background: To evaluate the outcomes of pancreaticogastrostomy and pancreaticojejunostomy after pancreatoduodenectomy with the help of a meta-analysis.

Methods: Randomized controlled trials comparing pancreaticogastrostomy and pancreaticojejunostomy were searched electronically using PubMed, The Cochrane Library, and EMBASE. Fixed and random-effects were used to measure pooled estimates. Research indicators included pancreatic fistula, delayed gastric emptying, postoperative hemorrhage, intraperitoneal fluid collection, wound infection, overall postoperative complications, reoperation, and mortality.

Results: Overall, 10 randomized controlled trials were included in this meta-analysis, with a total of 1629 patients. The overall incidences of pancreatic fistula and intra-abdominal collections were lower in the pancreaticogastrostomy group than in the pancreaticojejunostomy group (OR=0.73, 95% CI 0.55~0.96, p=0.02; OR=0.59, 95% CI 0.37~0.96, p=0.02, respectively). The incidence of B/C grade pancreatic fistula in the pancreaticogastrostomy group was lower than that in the pancreaticojejunostomy group, but no significant difference was observed (OR=0.61, 95%CI 0.34~1.09, p=0.09). Postoperative hemorrhage was more frequent in the pancreaticogastrostomy group than in the pancreaticojejunostomy group (OR=1.52; 95% CI 1.08~2.14, p=0.02). No significant differences in terms of delayed gastric emptying, wound infection, reoperation, overall postoperative complications, mortality, exocrine function, and hospital readmission were observed between groups.

Conclusion: This meta-analysis suggests that pancreaticogastrostomy reduces the incidence of postoperative pancreatic fistula and intraperitoneal fluid collection but increases the risk of postoperative hemorrhage compared with pancreaticojejunostomy.
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http://dx.doi.org/10.1186/s12957-021-02314-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262038PMC
July 2021

Pancreas-preserving duodenectomy for treatment of a duodenal papillary tumor: A case report.

World J Clin Cases 2021 Jun;9(18):4748-4753

Department of General Surgery, Gansu Provincial Hospital, Lanzhou 730000, Gansu Province, China.

Background: Duodenal papillary tumor is a rare tumor of the digestive tract, accounting for about 0.2% of gastrointestinal tumors and 7% of periampullary tumors. The clinical manifestations of biliary obstruction are most common. Some benign tumors or small malignant tumors are often not easily found because they have no obvious symptoms in the early stage. Surgical resection is the only treatment for duodenal papillary tumors. At present, the methods of operation for duodenal papillary tumors include pancreatoduodenectomy, duodenectomy, ampullectomy, and endoscopic resection.

Case Summary: A 47-year-old man was admitted to because of a duodenal mass that had been discovered 2 mo previously. Electronic gastroscopy at another hospital revealed a duodenal papillary mass that had been considered to be a high-grade intraepithelial neoplasia. Therefore, we conducted a multidisciplinary group discussion and decided to perform a pancreas-preserving duodenectomy and a R0 resection was successfully performed. After surgery, the patient underwent a follow-up period of 5 yr. No recurrence or metastasis occurred.

Conclusion: According to our experience with a duodenal papillary tumor, compared with pancreaticoduodenectomy, the use of pancreas-preserving duodenectomy can preserve pancreatic function, maintain gastrointestinal structure and function, reduce tissue damage and complications, and render the postoperative recovery faster. Pancreas-preserving duodenectomy for treatment of a duodenal papillary tumor is feasible under strict control of surgical indications.
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http://dx.doi.org/10.12998/wjcc.v9.i18.4748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223821PMC
June 2021

[First Extended Anaerobic Phase Enhanced Nitrogen and Phosphorus Removal by Aerobic Granular Sludge Under Intermittent Gradient Aeration].

Huan Jing Ke Xue 2021 Jul;42(7):3405-3412

Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.

In this study, a SBR reactor was selected to explore the effect of nitrogen and phosphorus removal by aerobic granular sludge under different initial anaerobic time using intermittent gradient aeration (decreasing dissolved oxygen concentration in each aeration section) and actual domestic wastewater with low C/N ratio as the influent matrix. The results showed that the initial anaerobic stage of intermittent gradient aeration increased from 50 min to 90 min, which increased the carbon source reserves in granular sludge. This improved delayed anaerobic condition led to the improvement of nitrogen and phosphorus removal efficiency of granular sludge. When delayed to 70 and 90 min, the removal efficiency of COD, TN, and TP reached 84.74%, 70.05%, and 89.7% and 86.65%, 78.81%, and 85.5%, respectively. However, after the first anaerobic phase time was increased to 110 min, the sludge loss was more severe owing to the disintegration of some cells, leading to a decrease in internal carbon source reserves by about 13.6%. Owing to this, the pollutant removal efficiency decreased. In the process of prolonging the first anaerobic phase time from 50 min to 90 min, the content of PS in LB-EPS changed minimally; when it was delayed to 110 min, the content of PS increased to about 7.18 mg·g, and the PN content increased to about 5.56 mg·g. The contents of PN and PS in TB-EPS were stable, which indicated that the effect of internal carbon storage on LB-EPS was greater than that of TB-EPS, and the decrease in sludge settling performance was closely related to the increase in PS content in LB-EPS. The proportion of DPAOs in granular sludge increased as the first anaerobic phase time increased, and the proportion of DPAOs reached 51.5% when the first anaerobic phase time was 70 min.
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http://dx.doi.org/10.13227/j.hjkx.202011124DOI Listing
July 2021

Sex influences the association between appendicular skeletal muscle mass to visceral fat area ratio and non-alcoholic steatohepatitis in patients with biopsy-proven non-alcoholic fatty liver disease.

Br J Nutr 2021 Jun 28:1-8. Epub 2021 Jun 28.

NAFLD Research Center, Department of Hepatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou325000, People's Republic of China.

Sarcopenic obesity is regarded as a risk factor for the progression and development of non-alcoholic fatty liver disease (NAFLD). Since male sex is a risk factor for NAFLD and skeletal muscle mass markedly varies between the sexes, we examined whether sex influences the association between appendicular skeletal muscle mass to visceral fat area ratio (SVR), that is, an index of skeletal muscle mass combined with abdominal obesity, and the histological severity of NAFLD. The SVR was measured by bioelectrical impedance in a cohort of 613 (M/F = 443/170) Chinese middle-aged individuals with biopsy-proven NAFLD. Multivariable logistic regression and subgroup analyses were used to test the association between SVR and the severity of NAFLD (i.e. non-alcoholic steatohepatitis (NASH) or NASH with the presence of any stage of liver fibrosis). NASH was identified by a NAFLD activity score ≥5, with a minimum score of 1 for each of its categories. The presence of fibrosis was classified as having a histological stage ≥1. The SVR was inversely associated with NASH in men (adjusted OR 0·62; 95 % CI 0·42, 0·92, P = 0·017 for NASH, adjusted OR 0·65; 95 % CI 0·43, 0·99, P = 0·043 for NASH with the presence of fibrosis), but not in women (1·47 (95 % CI 0·76, 2·83), P = 0·25 for NASH, and 1·45 (95 % CI 0·74, 2·83), P = 0·28 for NASH with the presence of fibrosis). There was a significant interaction for sex and SVR (Pinteraction = 0·017 for NASH and Pinteraction = 0·033 for NASH with the presence of fibrosis). Our findings show that lower skeletal muscle mass combined with abdominal obesity is strongly associated with the presence of NASH only in men.
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http://dx.doi.org/10.1017/S0007114521002415DOI Listing
June 2021

NADPH is superior to NADH or edaravone in ameliorating metabolic disturbance and brain injury in ischemic stroke.

Acta Pharmacol Sin 2021 Jun 24. Epub 2021 Jun 24.

Department of Pharmacology and Laboratory of Aging and Nervous Diseases, Jiangsu Key Laboratory of Neuropsychiatric Diseases, College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, China.

Our previous studies confirm that exogenous reduced nicotinamide adenine dinucleotide phosphate (NADPH) exerts a neuroprotective effect in animal models of ischemic stroke, and its primary mechanism is related to anti-oxidative stress and improved energy metabolism. However, it is unknown whether nicotinamide adenine dinucleotide (NADH) also plays a neuroprotective role and whether NADPH is superior to NADH against ischemic stroke? In this study we compared the efficacy of NADH, NADPH, and edaravone in ameliorating brain injury and metabolic stress in ischemic stroke. Transient middle cerebral artery occlusion/reperfusion (t-MCAO/R) mouse model and in vitro oxygen glucose deprivation/reoxygenation (OGD/R) model were established. The mice were intravenously administered the optimal dose of NADPH (7.5 mg/kg), NADH (22.5 mg/kg), or edaravone (3 mg/kg) immediately after reperfusion. We showed that the overall efficacy of NADPH in ameliorating ischemic injury was superior to NADH and edaravone. NADPH had a longer therapeutic time window (within 5 h) after reperfusion than NADH and edaravone (within 2 h) for ischemic stroke. In addition, NADPH and edaravone were better in alleviating the brain atrophy, while NADH and NADPH were better in increasing the long-term survival rate. NADPH showed stronger antioxidant effects than NADH and edaravone; but NADH was the best in terms of maintaining energy metabolism. Taken together, this study demonstrates that NADPH exerts better neuroprotective effects against ischemic stroke than NADH and edaravone.
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http://dx.doi.org/10.1038/s41401-021-00705-5DOI Listing
June 2021

Exploring the Coordination Modes of a Keggin-Type [ZnWO] Anionic Cluster: Bonding Patterns, Crystal Structure, and Semiconducting Properties.

Inorg Chem 2021 Jun 2;60(12):9097-9109. Epub 2021 Jun 2.

Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education, Faculty of Chemistry, Northeast Normal University, Changchun, Jilin 130024, People's Republic of China.

Polyoxometalate-based organic-inorganic hybrid compounds (POIHCs) have been greatly developed due to their wide application prospects, but the pursuit of their directed synthesis via molecular design still remains a challenge. Herein, we demonstrate that the coordination modes of the Keggin-type [ZnWO] anion can be tuned, which leads to different semiconductor characteristics. Using the same building block, ligand, and metal ion (ZnW, phen, Cu), we synthesized three new POIHCs with different bonding patterns by means of different coordination modes of ZnW. The three POIHCs (Hphen){ZnWO[Cu(phen)]}·3HO (), {ZnWO[Cu(phen)(HO)][Cu(phen)(HO)]}·3HO (), and (MeN){ZnWO[Cu(phen)(HO)]}·5HO () (phen = 1,10-phenanthroline) have been structurally characterized by single-crystal X-ray diffraction. Compound appears as a zero-dimensional coordination complex cluster, while compounds and are both 1D chain structures with different Cu bridge linkages. Although these three POIHCs possess the same chemical components, their semiconductor properties are different, which is demonstrated by measurements of transient photocurrent and band gap () values. Furthermore, we carried out comparative experiments on the photoconductivity performance of compounds - and their photocatalytic reduction from O to HO, indicating the significant influence of the energy level matching on the photocatalytic activity.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01098DOI Listing
June 2021

TA allele of rs2070673 in the CYP2E1 gene is associated with lobular inflammation and nonalcoholic steatohepatitis in patients with biopsy-proven nonalcoholic fatty liver disease.

J Gastroenterol Hepatol 2021 May 24. Epub 2021 May 24.

NAFLD Research Center, Department of Hepatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background And Aim: Cytochrome P450 2E1 (CYP2E1) plays a role in lipid metabolism, and by increasing hepatic oxidative stress and inflammation, the upregulation of CYP2E1 is involved in development of nonalcoholic steatohepatitis (NASH). We aimed to explore the relationship between CYP2E1-333A>T (rs2070673) and the histological severity of nonalcoholic fatty liver disease (NAFLD).

Methods: We studied 438 patients with biopsy-proven NAFLD. NASH was defined as NAFLD Activity Score ≥ 5 with existence of steatosis, ballooning, and lobular inflammation. CYP2E1-333A>T (rs2070673) was genotyped by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Serum cytokines related to inflammation were measured by the Bio-plex 200 system to investigate possible mediating factors involved in the process.

Results: The TA genotype of rs2070673 had a higher prevalence of moderate/severe lobular inflammation (27.6% vs 20.3% vs 13.3%, P < 0.01) and NASH (55.7% vs 42.4% vs 40.5%, P < 0.01) compared with the AA and TT genotypes, respectively. In multivariable regression modeling, the heterozygote state TA was associated with moderate/severe lobular inflammation (adjusted odds ratio: 2.31, 95% confidence interval 1.41-3.78, P < 0.01) or NASH (adjusted odds ratio: 1.82, 95% confidence interval 1.22-2.69, P < 0.01), independently of age, sex, common metabolic risk factors, and presence of liver fibrosis. Compared with no-NASH, NASH patients had significantly higher levels of serum interleukin-1 receptor antagonist, interleukin-18, and interferon-inducible protein-10 (IP-10), whereas only IP-10 was increased with the rs2070673 TA variant (P = 0.01). Mediation analysis showed that IP-10 was responsible for ~60% of the association between the rs2070672 and NASH.

Conclusions: The TA allele of rs2070673 is strongly associated with lobular inflammation and NASH, and this effect appears to be largely mediated by serum IP-10 levels.
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http://dx.doi.org/10.1111/jgh.15554DOI Listing
May 2021

Electric field controlled type-I and type-II conversion of BP/SnS van der Waals heterostructure.

J Phys Condens Matter 2021 May 25;33(26). Epub 2021 May 25.

Beijing Key Laboratory of Nanophotonics and Ultrafine Optoelectronic Systems, School of Physics, Beijing Institute of Technology, Zhongguancun South Street, Haidian District, Beijing 100081, People's Republic of China.

Type-I heterostructure, in which electrons and holes are confined in same region, is widely used in light emitting diodes and semiconductor lasers. Type-II heterostructure is widely used in photovoltaic devices because of its excellent spatial separation property of electrons and holes. Can we integrate photovoltaic, photoelectric properties with luminescent property in one device? Here we report a van der Waals heterostructure formed by black phosphorus (BP) and SnS monolayers. It is expected to realize these functions in one device. By first-principles methods, the structural stability, electronic properties and optical properties are investigated. It was found that the BP/SnS bilayer is type-II heterostructure with an indirect bandgap of 0.56 eV. The-like character of the band edge in BP/SnS vdW heterostructure makes it to be an excellent optoelectronic material. The type-II stability of the system can be improved by applying a negative electric field. However, when the positive electric field is bigger than 0.1 V Å, the system begins to transform from type-II to type I. Therefore, by adding a gate voltage the bandgap and band alignment of this system can be controlled. The photovoltaic and photoelectric properties can be integrated in one device based on this heterostructure.
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http://dx.doi.org/10.1088/1361-648X/abfc15DOI Listing
May 2021

[Adsorption of BS-18 Amphoterically Modified Bentonite to Tetracycline and Norfloxacin Combined Pollutants].

Huan Jing Ke Xue 2021 May;42(5):2334-2342

College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China.

Antibiotic pollution in the environment has become a hot topic. The amphoteric surfactant octadecyl dimethyl betaine (BS-18) was adopted to modify bentonite to investigate the effects and mechanisms of the composite adsorption of different types of antibiotics. Under the different modification ratios, temperatures, pH values, and ionic strength conditions, the adsorption of tetracycline (TC) and norfloxacin (NOR) by bentonite was studied under single and compound conditions, and the adsorption mechanism was analyzed and discussed in combination with the surface properties of amphoterically modified bentonite. The results showed that compared with those of CK, the CEC and specific surface area of the soil samples modified by BS-18 decreased, whereas the total carbon and total nitrogen contents increased. The adsorption order of BS-18 amphoterically modified bentonite to TC was CK > 100BS > 25BS > 50BS, which was in accordance with the Langmuir model; the adsorption order of NOR was 25BS > 50BS > CK > 100BS, which was consistent with the Henry model. The adsorption capacity of TC and NOR in the TC and NOR composite system was higher than that in the single system. With the increase in temperature, the adsorption of amphoterically modified bentonite to TC showed a positive warming effect, whereas the adsorption of NOR declined as the temperature increased. When the ionic strength increased from 0.001 mol·L to 0.500 mol·L, the adsorption of TC and NOR on each soil sample was inhibited. The pH of the solution can affect the existing forms of TC and NOR, and the adsorption capacity showed different trends as the pH increased. The adsorption of TC by BS-18-modified bentonite was mainly caused by electric charge attraction, whereas the adsorption of NOR was mainly caused by the combination of electric charge attraction and the hydrophobic effect. The different values of the octanol/water partition coefficient and the difference in structure resulted in different adsorption modes. In the TC and NOR composite system, a TC+NOR mixture was formed to promote the adsorption of soil samples.
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http://dx.doi.org/10.13227/j.hjkx.202009048DOI Listing
May 2021

Association between psychological capital and spiritual care competencies of clinical nurses: A multicentre cross-sectional study.

J Nurs Manag 2021 Mar 7. Epub 2021 Mar 7.

School of Nursing, Hebei University, Baoding, China.

Aims: To investigate the status of spiritual care competencies among clinical nurses and their relationships with psychological capital.

Background: Limited knowledge is about the influence of positive personal characteristics on nurses' spiritual care competencies.

Methods: A multicentre cross-sectional study. A total of 1717 nurses were recruited from nine separate Chinese hospitals. Online questionnaires were delivered through a local nursing association to assess socio-demographics, spiritual care competencies and psychological capital of nurses.

Results: Nurses had mild-to-moderate levels of spiritual care competencies and moderate levels of psychological capital. Psychological capital and its two metrics (self-efficacy and hope), spiritual care education, professional qualification and shift work were the main predictors of spiritual care competencies (each p < .05).

Conclusion: The findings of the study show a positive relationship between psychological capital and spiritual care competencies of clinical nurses. Strengthening nurses' psychological capital could improve their spiritual care competencies.

Implications For Nursing Managers: Nurse managers and hospital administrators should better understand the value of psychological capital for nurses' capacity development. Effective interventions need to be implemented separately or combined with spiritual care education programmes to improve nurses' psychological capital and spiritual care competencies.
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http://dx.doi.org/10.1111/jonm.13303DOI Listing
March 2021

Progress on the GntR family transcription regulators in bacteria.

Yi Chuan 2021 Jan;43(1):66-73

College of Life Science and Technology, Guangxi University, Nanning 530004, China.

In bacteria, GntR family transcription regulators are the widespread family of transcription factors. Members of this family consist of two functional domains, a conserved N-terminal DNA-binding domain that contains a typical helix-turn-helix (HTH) motif and a C-terminal effector-binding or oligomerization domain. Usually, the amino acid sequences of N-terminal DNA-binding domains are highly conserved, but differ in the C-terminal effector-binding or oligomerization domains. In the past several decades, many GntR family transcription regulators have been characterized in a number of bacteria. These regulators control a variety of cellular processes such as cell motility, glucose metabolism, bacterial resistance, pathogenesis and virulence. In this review, we summarized the discovery, C-terminal domains, biological function and regulation mode of GntR family transcription regulators. This review will help researchers to obtain more knowledge about the functions and mechanisms of the GntR family transcriptional regulatory factors.
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http://dx.doi.org/10.16288/j.yczz.20-245DOI Listing
January 2021

A recombinant influenza virus with a CTLA4-specific scFv inhibits tumor growth in a mouse model.

Cell Biol Int 2021 Jun 1;45(6):1202-1210. Epub 2021 Mar 1.

Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.

Oncolytic viruses (OV) have shown excellent safety and efficacy in preclinical and clinical studies. Influenza A virus (IAV) is considered a promising oncolytic virus. In this report, we generated a recombinant influenza virus expressing an immune checkpoint blockade agent targeting CTLA4. Using reverse genetics, a recombinant influenza virus, termed rFlu-CTLA4, encoding the heavy chain of a CTLA4 antibody on the PB1 segment and the light chain of the CTLA4 antibody on the PA segment was produced. RFlu-CTLA4 could replicate to high titers, and antibodies were produced in the allantoic fluid of infected eggs. Furthermore, the selective cytotoxicity of the virus was higher in various hepatocellular carcinoma cancer cell lines than in the normal cell line L02 in vitro, as indicated by MTS assays. More importantly, in a subcutaneous H22 mouse hepatocarcinoma model, intratumoral injections of rFlu-CTLA4 inhibited the growth of treated tumors and increased the overall survival of mice compared with injections of the PR8 virus. Taken together, these results warrant further exploration of this novel recombinant influenza virus for its potential use as a single or combination agent for cancer immunotherapy.
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http://dx.doi.org/10.1002/cbin.11559DOI Listing
June 2021

Impaired meningeal lymphatic drainage in patients with idiopathic Parkinson's disease.

Nat Med 2021 03 18;27(3):411-418. Epub 2021 Jan 18.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Animal studies implicate meningeal lymphatic dysfunction in the pathogenesis of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease (PD). However, there is no direct evidence in humans to support this role. In this study, we used dynamic contrast-enhanced magnetic resonance imaging to assess meningeal lymphatic flow in cognitively normal controls and patients with idiopathic PD (iPD) or atypical Parkinsonian (AP) disorders. We found that patients with iPD exhibited significantly reduced flow through the meningeal lymphatic vessels (mLVs) along the superior sagittal sinus and sigmoid sinus, as well as a notable delay in deep cervical lymph node perfusion, compared to patients with AP. There was no significant difference in the size (cross-sectional area) of mLVs in patients with iPD or AP versus controls. In mice injected with α-synuclein (α-syn) preformed fibrils, we showed that the emergence of α-syn pathology was followed by delayed meningeal lymphatic drainage, loss of tight junctions among meningeal lymphatic endothelial cells and increased inflammation of the meninges. Finally, blocking flow through the mLVs in mice treated with α-syn preformed fibrils increased α-syn pathology and exacerbated motor and memory deficits. These results suggest that meningeal lymphatic drainage dysfunction aggravates α-syn pathology and contributes to the progression of PD.
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http://dx.doi.org/10.1038/s41591-020-01198-1DOI Listing
March 2021

Effect of postoperative early enteral nutrition on clinical outcomes and immune function of cholangiocarcinoma patients with malignant obstructive jaundice.

World J Gastroenterol 2020 Dec;26(46):7405-7415

Department of General Surgery, Gansu Provincial Hospital, Lanzhou 730000, Gansu Province, China.

Background: Most cholangiocarcinoma patients with malignant obstructive jaundice (MOJ) have varying degrees of malnutrition and immunodeficiency preoperatively. Therefore, perioperative nutritional support has important clinical significance in the treatment of cholangiocarcinoma.

Aim: To investigate the effects of postoperative early enteral nutrition (EEN) on immunity function and clinical outcomes of cholangiocarcinoma patients with MOJ.

Methods: This prospective clinical study included 60 cholangiocarcinoma patients with MOJ who underwent surgery. The patients were randomly divided into an experimental group and a control group according to the nutrition support modes. The control group received postoperative total parenteral nutrition (TPN), whereas the experimental group received postoperative EEN and parenteral nutrition (PN; EEN + PN). The clinical outcomes, postoperative immune function, incidences of surgical site infection and bile leakage, intestinal function recovery time, average hospitalization days, and hospitalization expenses of the two groups were assessed on postoperative days (PODs) 1, 3, and 7.

Results: The CD3+T, CD4+T, CD8+T, and CD4+T/CD8+T cell count and the immunoglobulin (Ig) G, IgM, and IgA levels in the EEN + PN group were significantly higher than those in the TPN group on PODs 3 and 7 ( < 0.05), whereas no significant differences in the CD3+T, CD4+T, CD8+T, and CD4+T/CD8+T cell counts and IgG, IgM, and IgA levels before operation and on POD 1 were found between the two groups ( > 0.05). The intestinal function recovery time and postoperative hospital stay were shorter ( < 0.001 for both) in the EEN + PN group than in the TPN group. The hospitalization expenses of the EEN + PN group were lower than those of the TPN group ( < 0.001). However, the incidence of abdominal distension was higher than in the EEN + PN group than in the TPN group ( < 0.05). The incidence rates of biliary leakage and surgical site infection were not significantly different between the two groups ( > 0.05).

Conclusion: A postoperative EEN program could reduce the incidence of postoperative complications and improve the clinical outcomes and immune functions of cholangiocarcinoma patients with MOJ and is thus beneficial to patient recovery.
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http://dx.doi.org/10.3748/wjg.v26.i46.7405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7739166PMC
December 2020

Emergency surgical workflow and experience of suspected cases of COVID-19: A case report.

World J Clin Cases 2020 Nov;8(21):5361-5370

Department of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.

Background: Severe acute respiratory syndrome coronavirus 2 has been confirmed to be a newly discovered zoonotic pathogen that causes highly contagious viral pneumonia, which the World Health Organization has named novel coronavirus pneumonia. Since its outbreak, it has become a global pandemic. During the outbreak of coronavirus disease 2019 (COVID-19), however, there is no mature experience or guidance on how to carry out emergency surgery for suspected cases requiring emergency surgical intervention and perioperative safety protection against virus.

Case Summary: A 41-year-old man was admitted to the hospital for emergency treatment due to "3-d abdominal pain aggravated with cessation of exhaust and defecation". After improving inspections and laboratory tests, the patient was assessed and diagnosed by the multiple discipline team as "strangulation obstruction, pulmonary infection". His body temperature was 38.8 °C, and the chest computed tomography showed pulmonary infection. Given fever and pneumonia, we could not rule out COVID-19 after consultation by fever clinicians and respiratory experts. Hence, we performed emergency surgery under three-level protection for the suspected case. After surgery, his nucleic acid test for COVID-19 was negative, meaning COVID-19 was excluded, and routine postoperative treatment and nursing was followed. The patient was treated with symptomatic support after the operation. The stomach tube and urinary tube were removed on the 1 d after the operation. The clearing diet was started on the 3 d after the operation, and the body temperature returned to normal. Flatus and bowel movements were noted on 5 postoperative day. He was discharged after 8 d of hospitalization. The patient was followed up for 4 mo after discharge, no serious complications occurred. A 71-year-old woman was admitted to our emergency room due to "abdominal distention, fatigue for 6 d and fever for 13 h". After the multiple discipline team evaluation, the patient was diagnosed as "intestinal obstruction, abdominal mass, peritonitis and pulmonary infection". At that time, the patient's body temperature was 39.6 °C, and chest computed tomography indicated pulmonary infection. COVID-19 could not be completely excluded after consultation in the fever outpatient department and respiratory department. Therefore, the patient was treated as a suspected case, and an urgent operation was performed under three-level medical protection. Postoperative nucleic acid test was negative, COVID-19 was excluded, and routine postoperative treatment and nursing were followed. After the operation, the patient received symptomatic and supportive treatment. The gastric tube was removed on the 1 d after the operation, and the urinary tube was removed on the 3 d after the operation. Enteral nutrition began on the 3 d after the operation. To date, no serious complications have been found during follow-up after discharge.

Conclusion: Based on the previous treatment experience, we reviewed the procedures of two cases of suspected COVID-19 emergency surgery and extracted the perioperative protection experience. By referring to the literature and following the regulations on prevention and management of infectious diseases, we have developed a relatively mature and complete emergency surgical workflow for suspected COVID-19 cases and shared perioperative protection and management experience and measures.
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http://dx.doi.org/10.12998/wjcc.v8.i21.5361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674731PMC
November 2020

Effects of early oral feeding after radical total gastrectomy in gastric cancer patients.

World J Gastroenterol 2020 Sep;26(36):5508-5519

Department of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.

Background: Gastric cancer (GC) is a heavy burden in China. Nutritional support for GC patients is closely related to postoperative rehabilitation. However, the role of early oral feeding after laparoscopic radical total gastrectomy in GC patients is unclear and high-quality research evidence is scarce.

Aim: To prospectively explore the safety, feasibility and short-term clinical outcomes of early oral feeding after laparoscopic radical total gastrectomy for GC patients.

Methods: This study was a prospective cohort study conducted between January 2018 and December 2019 based in a high-volume tertiary hospital in China. A total of 206 patients who underwent laparoscopic radical total gastrectomy for GC were enrolled. Of which, 105 patients were given early oral feeding (EOF group) after surgery, and the other 101 patients were given the traditional feeding strategy (control group) after surgery. Perioperative clinical data were recorded and analyzed. The primary endpoints were gastrointestinal function recovery time and postoperative complications, and the secondary endpoints were postoperative nutritional status, length of hospital stay and expenses, .

Results: Compared with the control group, patients in the EOF group had a significantly shorter postoperative first exhaust time (2.48 ± 1.17 d 3.37 ± 1.42 d, = 0.001) and first defecation time (3.83 ± 2.41 d 5.32 ± 2.70 d, = 0. 004). In addition, the EOF group had a significant shorter postoperative hospitalization duration (5.85 ± 1.53 d 7.71 ± 1.56 d, < 0.001) and lower postoperative hospitalization expenses (16.60 ± 5.10 K¥ 21.00 ± 7.50 K¥, = 0.014). On the 5 day after surgery, serum prealbumin level (214.52 ± 22.47 mg/L 204.17 ± 20.62 mg/L, = 0.018), serum gastrin level (246.30 ± 57.10 ng/L 223.60 ± 55.70 ng/L, = 0.001) and serum motilin level (424.60 ± 68.30 ng/L 409.30 ± 61.70 ng/L, = 0.002) were higher in the EOF group. However, there was no significant difference in the incidence of total postoperative complications between the two groups ( = 0.507).

Conclusion: Early oral feeding after laparoscopic radical total gastrectomy can promote the recovery of gastrointestinal function, improve postoperative nutritional status, reduce length of hospital stay and expenses while not increasing the incidence of related complications, which indicates its safety, feasibility and potential benefits for gastric cancer patients.
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http://dx.doi.org/10.3748/wjg.v26.i36.5508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520607PMC
September 2020

A sphingosine kinase 2-mimicking TAT-peptide protects neurons against ischemia-reperfusion injury by activating BNIP3-mediated mitophagy.

Neuropharmacology 2020 12 20;181:108326. Epub 2020 Sep 20.

Department of Pharmacology and Laboratory of Aging and Nervous Diseases, Jiangsu Key Laboratory of Neuropsychiatric Diseases, College of Pharmaceutical Sciences of Soochow University, Suzhou, China. Electronic address:

We have previously shown that sphingosine kinase 2 (SPK2) interacts with Bcl-2 via its BH3 domain, activating autophagy by inducing the dissociation of Beclin-1/Bcl-2 complexes, and that a TAT-SPK2 peptide containing the BH3 domain of SPK2 protects neurons against ischemic injury. The goals of the present study were to establish the functional significance of these findings, by testing whether TAT-SPK2 was effective in a mouse model of ischemic stroke, and to explore potential underlying mechanisms. Mice were administered with TAT-SPK2 by intraperitoneal injection before or after transient middle cerebral artery occlusion (tMCAO). Infarct volume, neurological deficit and brain water content were assessed 24 h after reperfusion. Mitophagy inhibitor Mdivi-1 and BNIP3 siRNAs were used to examine the involvement of BNIP3-dependent mitophagy in the neuroprotection of TAT-SPK2. Mitophagy was quantified by immunoblotting, immunofluorescence and electron microscopy. The interaction between TAT-SPK2 and Bcl-2, Bcl-2 and BNIP3 was detected by co-immunoprecipitation. In the tMCAO model, pre-treatment with TAT-SPK2 significantly reduced infarct volume, improved neurological function and decreased brain edema. Neuroprotection by TAT-SPK2 was still seen when the peptide was administered 3 h after reperfusion. TAT-SPK2 also significantly improved functional recovery and reduced long-term brain atrophy of the ischemic hemisphere 30 days after administration. Our studies further showed that TAT-SPK2 directly binds to Bcl-2 and disrupts Bcl-2/Beclin-1 or Bcl-2/BNIP3 complexes to induce mitophagy. These results suggest that TAT-SPK2 protects neurons against ischemia reperfusion injury by activating BNIP3-mediated mitophagy. Agents exploiting this molecular mechanism are potential candidates for the treatment of ischemic stroke.
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http://dx.doi.org/10.1016/j.neuropharm.2020.108326DOI Listing
December 2020

Widely tunable direct bandgap of two-dimensional GeSe.

J Phys Condens Matter 2020 Mar;33(11):115301

Bulk GeSe is an indirect bandgap semiconductor. However, direct bandgap semiconductor of two-dimensional GeSe can be obtained by applying strain along armchair direction, and the direct bandgap can be tuned in a wide energy range from 0.86 eV to 0.00 eV by electric field. The bandgap modulation mechanism is studied in detail by first-principle calculations. The calculations of phonon spectra show that the crystal structure is relatively stable under the strain and electric field. Therefore, 2D GeSe is a promising material in frequency adjustable electronic and optical devices.
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http://dx.doi.org/10.1088/1361-648X/abba66DOI Listing
March 2020

Role of prophylactic hyperthermic intraperitoneal chemotherapy in patients with locally advanced gastric cancer.

World J Gastrointest Oncol 2020 Jul;12(7):782-790

Department of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.

Background: Gastric cancer is the second most common malignant tumor in China, ranking third among all malignant tumor mortality rates. Hyperthermic intraperitoneal chemotherapy (HIPEC) has been shown to increase significantly the effectiveness of intraperitoneal chemotherapeutic drugs, prolong the action time of these drugs on intraperitoneal tumor cells, and enhance their diffusion in tumor tissues. HIPEC may be one of the best choices for the eradication of residual cancer cells in the abdominal cavity.

Aim: The aim of this study was to study the role of preventive HIPEC after radical gastrectomy.

Methods: A prospective analysis was performed with patients with cTNM gastric cancer to compare the effects of postoperative prophylactic HIPEC plus intravenous chemotherapy with those of routine adjuvant chemotherapy. Patients' medical records were analyzed, and differences in the peritoneal recurrence rate, disease-free survival time, and total survival time between groups were examined.

Results: The first site of tumor recurrence was the peritoneum in 11 cases in the conventional adjuvant chemotherapy group and in 2 cases in the HIPEC group ( = 0.020). The 1-year and 3-year disease-free survival rates were 91.9% and 60.4%, respectively, in the conventional adjuvant chemotherapy group and 92.1% and 63.0%, respectively, in the HIPEC group. The 1-year and 3-year overall survival rates were 95.2% and 66.3%, respectively, in the conventional adjuvant chemotherapy group and 96.1% and 68.6%, respectively, in the HIPEC group. No significant difference in postoperative or chemotherapy complications was observed between groups.

Conclusion: In patients with cTNM gastric cancer, prophylactic HIPEC after radical tumor surgery is beneficial to reduce peritoneal tumor recurrence and prolong survival.
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http://dx.doi.org/10.4251/wjgo.v12.i7.782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428793PMC
July 2020

Histopathological features of multiorgan percutaneous tissue core biopsy in patients with COVID-19.

J Clin Pathol 2021 Aug 26;74(8):522-527. Epub 2020 Aug 26.

Department of Pathology, Beijing Youan Hospital, Capital Medical University, Beijing, China

Aims: The global outbreak of COVID-19 has resulted in an increased mortality. However, whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can affect multiple organs is still unclear. In this study, postmortem percutaneous biopsies of multiple organs from deceased patients were performed to understand the histopathological changes caused by COVID-19.

Methods: Biopsy specimens of pulmonary, cardiac, hepatic and lymphoid tissues were obtained from three patients, who died due to COVID-19 pneumonia. H&E stain, Masson trichrome stain, immunohistochemistry stain and in-situ hybridisation were used.

Results: Pulmonary damages caused by SARS-CoV-2 infection was diffuse alveolar damage (DAD). In the early phase, the histological findings were mainly those of exudative features of DAD. The later phase was characterised by organisation of DAD combined with bacterial pneumonia. No serious damage was found in the bronchiolar epithelium and submucosal glands. The hepatic tissue revealed features of ischaemic necrosis, but findings suggestive of mild lobular hepatitis were also observed. The lymphoid tissue revealed features of non-specific acute lymphadenitis. The cardiac tissue revealed changes of underlying disease. SARS-CoV-2 RNAs were not detected in hepatocytes, cholangiocytes and lymphocytes of lymph nodes.

Conclusions: COVID-19 predominantly involves the pulmonary tissue, causes DAD and aggravates the cardiovascular disease. However, other extrapulmonary tissues did not reveal any virus-specific findings, but were affected by multiple factors. The findings in this report caution the pathologists that they should not mistakenly attribute all the histological features to CoV infection. Moreover, the clinicians should pay attention to the potentially injurious and correctable causes.
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http://dx.doi.org/10.1136/jclinpath-2020-206623DOI Listing
August 2021

Liver stiffness measurement is a potent predictor of histological fibrosis regression after hepatitis C virus clearance.

Eur J Gastroenterol Hepatol 2021 Apr;33(4):547-554

Department of Hepatitis C and Drug-Induced Liver Injury, Beijing Youan Hospital, Capital Medical University, Beijing, China.

Background: Most of the studies on fibrosis regression prediction were based on noninvasive fibrosis markers and differ greatly. The 'Beijing fibrosis classification' can use histological results to classify fibrosis into progressive or 'nonprogressive' according to fibrotic septal morphology. We use this standard which served as the gold standard in order to find fibrosis regression predictors.

Aim: To study the predictors of fibrosis regression after hepatitis C virus clearance according to histological fibrosis staging by the 'Beijing fibrosis classification'.

Materials And Methods: This was a prospective cohort study. A total of 68 patients with advanced liver fibrosis or compensated cirrhosis who achieved sustained virological response were enrolled. Patients with the Ishak scores lower than 3 seemed to have fibrosis regression. The others were divided into the fibrosis progressive group and the nonprogressive group according to the 'Beijing fibrosis classification'. Predictors of fibrosis regression were studied by logistic regression using baseline factors and the dynamic change in noninvasive fibrosis factors.

Results: Eighteen patients were assigned to the progressive group, and the others were assigned to the nonprogressive group. The baseline liver stiffness measurements (LSMs) of the progressive and nonprogressive groups were 14.35 (11.3, 27.3) kPa and 11.3 (8.3, 14.2) kPa, respectively, P = 0.02. The baseline LSM was the only predictor of fibrosis progression. With a cutoff of 11.85 kPa, the AUC was 0.71 (0.5, 0.9), and the negative predictive value was 0.92.

Conclusions: The baseline LSM was found to be the only predictor of fibrosis regression, 11.85 kPa is a possible 'hepatic fibrosis return point'.
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http://dx.doi.org/10.1097/MEG.0000000000001749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908855PMC
April 2021

High-Throughput Metabolomics for Identification of Metabolic Pathways and Deciphering the Effect Mechanism of Dioscin on Rectal Cancer From Cell Metabolic Profiles Coupled With Chemometrics Analysis.

Front Pharmacol 2020 28;11:68. Epub 2020 Feb 28.

Department of Orthopedics, The Affiliated First Hospital of Heilongjiang University of Chinese Medicine, Harbin, China.

High-throughput liquid chromatography-mass spectrometry (LC-MS)-based metabolomics can provide the holistic analysis of the low molecular weight endogenous metabolites in cells and reflect the changes of cellular regulation and metabolic pathways. Our study designed to reveal the potentially pharmacological effects of dioscin on SW480 rectal cancer cells using nontargeted metabolomics method to probe into small molecular metabolites and pathway changes. After the cell assay of proliferation, apoptosis, migration, and invasion, the dioscin-treated cell samples were prepared for nontargeted metabolomics analysis based on LC-MS tool to describe the metabolic profiles. Dioscin has prevented cell proliferation and accelerated cell apoptosis, and it also inhibited the SW480 rectal cancer cells' migration and invasion. A total of 22 metabolites were selected as promising biomarkers of pharmacological reaction of dioscin to rectal cancer, and eight highly correlated pathways including D-glutamine and D-glutamate metabolism, pyruvate metabolism, arachidonic acid metabolism, phenylalanine metabolism, tryptophan metabolism, glycolysis or gluconeogenesis, citrate cycle (TCA cycle), and butanoate metabolism were identified. It showed that strategies based on cell metabolomics are helpful tools to discover the small molecular metabolites to elucidate the action mechanism of drug.
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http://dx.doi.org/10.3389/fphar.2020.00068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059176PMC
February 2020

Comparison of electrolyte and glucose levels measured by a blood gas analyzer and an automated biochemistry analyzer among hospitalized patients.

J Clin Lab Anal 2020 Jul 9;34(7):e23291. Epub 2020 Mar 9.

Center of Clinical Laboratory, School of Medicine, Zhongshan Hospital, Xiamen University, Xiamen, China.

Background: Blood gas analyzers are capable of delivering results on electrolytes and metabolites within a few minutes and facilitate clinical decision-making. However, whether the results can be used interchangeably with values measured by chemistry analyzers remains controversial. Blood gas analyzers are capable of delivering results on electrolytes and metabolites within a few minutes and facilitate clinical decision-making. However, whether the results can be used interchangeably with values measured by chemistry analyzers remains controversial.

Methods: In total, arterial and matched venous blood samples were collected from 200 hospitalized patients. Arterial blood samples were evaluated using a RAPIDPOINT 500 to test electrolyte and glucose levels, then the samples were centrifuged and the same parameters were measured with an AU5800. Venous blood samples were processed and tested in accordance with standard operation procedures. Data were compared by using a paired t test, the agreement between the two analyzers was evaluated by using the Bland-Altman test, and sensitivity and specificity were calculated.

Results: Paired t tests showed that all parameters tested were significantly different between the two analyzers except chloride. The biases calculated indicated that blood gas analyzers tend to underestimate the parameters, and the linear regression showed a strong correlation between the two analyzers. The sensitivity, specificity and kappa values demonstrated that the diagnostic performance of blood gas analyzers is not satisfactory.

Conclusion: The significant reduction in parameter estimation and diagnostic performance we observed suggested that clinicians should interpret results from blood gas analyzers more cautiously. The reference interval of blood gas analyzers should be adjusted accordingly, given that values are underestimated.
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http://dx.doi.org/10.1002/jcla.23291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370720PMC
July 2020

A first-principles study on hydrogen distributions in the α-U/UO interface.

J Phys Condens Matter 2020 May;32(19):195002

LCP, Institute of Applied Physics and Computational Mathematics, Beijing 100094, People's Republic of China.

We investigate the distribution preference of hydrogen in the α-U/UO interface by using the DFT+U method. We find that a monolayer of hydrogen atoms firstly assembles right in the interface between α-U and UO. Through detailed electronic state analysis, it is revealed that the formation of U-H bonds with uranium atoms at the UO side favors this hydrogen atom assembly. The newly formed U-H bonds are similar to the U-O-U superexchange interactions in UO. The incorporation of hydrogen in either α-U or UO is much higher in energy. After formation of two hydrogen monolayers, following hydrogen atoms have no such preference in the interface area, and tend to distribute inside α-U.
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http://dx.doi.org/10.1088/1361-648X/ab6be7DOI Listing
May 2020

Xanthomonas campestris sensor kinase HpaS co-opts the orphan response regulator VemR to form a branched two-component system that regulates motility.

Mol Plant Pathol 2020 03 9;21(3):360-375. Epub 2020 Jan 9.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.

Xanthomonas campestris pv. campestris (Xcc) controls virulence and plant infection mechanisms via the activity of the sensor kinase and response regulator pair HpaS/hypersensitive response and pathogenicity G (HrpG). Detailed analysis of the regulatory role of HpaS has suggested the occurrence of further regulators besides HrpG. Here we used in vitro and in vivo approaches to identify the orphan response regulator VemR as another partner of HpaS and to characterize relevant interactions between components of this signalling system. Bacterial two-hybrid and protein pull-down assays revealed that HpaS physically interacts with VemR. Phos-tag SDS-PAGE analysis showed that mutation in hpaS reduced markedly the phosphorylation of VemR in vivo. Mutation analysis reveals that HpaS and VemR contribute to the regulation of motility and this relationship appears to be epistatic. Additionally, we show that VemR control of Xcc motility is due in part to its ability to interact and bind to the flagellum rotor protein FliM. Taken together, the findings describe the unrecognized regulatory role of sensor kinase HpaS and orphan response regulator VemR in the control of motility in Xcc and contribute to the understanding of the complex regulatory mechanisms used by Xcc during plant infection.
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http://dx.doi.org/10.1111/mpp.12901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036368PMC
March 2020

Motivators for and Barriers to Exercise Rehabilitation in Hemodialysis Centers: A Multicenter Cross-Sectional Survey.

Am J Phys Med Rehabil 2020 05;99(5):424-429

From the Department of Nephrology, China Rehabilitation Research Center, Beijing BoAi Hospital, Capital Medical University Rehabilitation Medical College, Beijing, China (X-XW, Z-HL, Y-CM); Department of Nephrology, Peking University People's Hospital, Beijing, China (YW); Hemodialysis Center, Beijing Zhan Lan Lu Hospital, Beijing, China (M-CX); Hemodialysis Center, General Hospital of China Aviation of Beijing, Beijing, China (Z-MK); and Hemodialysis Center, Chui Yang Liu Hospital affiliated to Tsinghua University, Beijing, China (WZ).

Objectives: The aim of the study was to explore motivators for and barriers to exercise rehabilitation in hemodialysis patients and the barriers perceived by the hemodialysis center staff.

Design: A cross-sectional study was performed in five hemodialysis centers using patient questionnaires designed for this study to evaluate the motivators for and barriers to exercise rehabilitation. Questionnaires were not yet validated.

Results: Of the 471 recruited patients, 63.3% were willing to participant in exercise rehabilitation. The greatest motivators included improving quality of life (98.0%) and wanting to be healthier (98.0%). Perceived barriers included discomfort (59.0%), concerns regarding safety (36.7%), and disinterest (27.0%). Among these, unwillingness, disinterest, and having peripheral arterial disease were independent risk factors of lack of participation in exercise rehabilitation. The most common perceived barriers among the 90 employees that participated were lack of professional guidance and advice from rehabilitation therapists (93.1%), lack of exercise rehabilitation knowledge (86.2%), and lack of special exercise equipment (86.2%).

Conclusions: Most patients were willing to exercise to improve their health and quality of life. Barriers to exercise rehabilitation included patient and staff factors. It is essential to establish a rehabilitation team within dialysis centers, including general staff and rehabilitation therapists. These centers require improved rehabilitation policies and access to specialized rehabilitation equipment.
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http://dx.doi.org/10.1097/PHM.0000000000001360DOI Listing
May 2020

[Application of energy cost in evaluating energy expenditure in multi-ball practice with table tennis players].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2019 Jul;35(4):331-335

China Table Tennis College, Shanghai University of Sport, Shanghai 200438, China.

Objective: To investigate the feasibility of applying the measure of energy cost, utilized widely in cyclic sports, in table tennis multi-ball practice.

Methods: Eleven collegiate table tennis players volunteered (18±1 yrs, 177±2 cm, 71±3 kg, approximately 10 yrs' training experience) to participate in one graded exercise test on treadmill, and two step tests (forehand and backhand, 3 min × 6, 35~85 stroke/min). A portable spirometric system and heart rate monitor were utilized for the three trials. Earlobe blood samples were collected and analyzed prior to and post the test. Energy cost was calculated for one stroke at each stroke frequency.

Results: The energy cost of loop drive multi-ball practice was decreased with increased stroke frequency (P<0.05). The energy cost of forehand loop drive was higher than backhand, with the difference significant at 35, 45, 55, 65, and 85 stroke·min (P<0.05). The function between energy cost and frequency were y=166.4x (R=0.9731), and y=33.21x (R=0.8423), respectively, where y was energy cost, and x was stroke frequency.

Conclusion: The measure of energy cost utilized in cyclic sports could be applied to evaluate the energy expenditure in table tennis multi-ball practice of single technique, and indicate the stroke efficiency of table tennis muti-ball practice with different stroke frequencies.
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http://dx.doi.org/10.12047/j.cjap.5778.2019.070DOI Listing
July 2019

Linear-shaped Ln and Ln clusters constructed by a polydentate Schiff base ligand and a β-diketone co-ligand: structures, fluorescence properties, magnetic refrigeration and single-molecule magnet behavior.

Dalton Trans 2019 Nov;48(44):16744-16755

Department of Chemistry, Taiyuan Normal University, Jinzhong 030619, China.

Herein, ten new linear-shaped LnIII4 and LnIII6 clusters, with the formula [Ln4(acac)6L2(CH3O)2(CH3OH)4]·xCH3OH (Ln = Nd (1), Sm (2), Eu (3), Gd (4), Tb (5), Dy (6), and Tm (8), Hacac = acetylacetone), [Ln6(acac)4L4(CH3O)6]·xCH3OH (Er (7) and Yb (9)), and [Lu4(acac)6L2(OH)2]·2CH2Cl2 (10), based on a polydentate Schiff base ligand, H2L, and a β-diketone co-ligand were successfully synthesized and structurally characterized. Single crystal X-ray diffraction measurements reveal that the structures of the clusters 1-6, 8 and 10 are very similar and their central Ln(iii) ions are linearly arranged Ln4; however, the clusters 7 and 9 possess a rare linearly arranged Ln6. The investigations on the solid-state fluorescence properties show that the clusters 2, 3, 5 and 6 display the characteristic lanthanum luminescence at room temperature. Magnetic studies reveal that weak antiferromagnetic interactions exist between adjacent Gd(iii) ions in cluster 4. More importantly, the cluster 4 exhibits significant MCE with the maximum -ΔSm value of 27.96 J kg-1 K-1 at 2.0 K and 7.0 T, whereas the cluster 6 displays a slow magnetic relaxation behavior under a zero dc field with the effective energy barrier ΔE/kB = 8.64 K and τ0 = 6.98 × 10-6 s.
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http://dx.doi.org/10.1039/c9dt03478aDOI Listing
November 2019

Hexamethylcucurbit[3,3]uril-Based Porous Supramolecular Assemblies and Their Adsorption Properties.

ACS Omega 2018 Aug 23;3(8):9827-9833. Epub 2018 Aug 23.

Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang 550025, People's Republic of China.

In the present work, we selected hexamethylcucurbit[3,3]uril (MeQ[3,3]) as a building block and obtained two MeQ[3,3]-based porous supramolecular assemblies from neutral water () and aqueous HCl solutions (), respectively. Both MeQ[3,3]-based assemblies are constructed of MeQ[3,3] molecules through the typical outer surface interaction of cucurbit[]urils, as well as hydrogen bonding between latticed water molecules and portal carbonyl oxygens of MeQ[3,3]. The assemblies present different porous structure features and exhibit different adsorption properties for eight common volatile organic compounds. However, the two porous assemblies exhibit similar adsorption properties for certain fluorophore dyes, including rhodamine B (), fluorescein (), and pyrene (), and form solid colored fluorescent compounds, some of which exhibit responses to the selected volatile organic compounds.
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http://dx.doi.org/10.1021/acsomega.8b00979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6644729PMC
August 2018

A novel self-sufficient biocatalyst based on transaminase and pyridoxal 5'-phosphate covalent co-immobilization and its application in continuous biosynthesis of sitagliptin.

Enzyme Microb Technol 2019 Nov 17;130:109362. Epub 2019 Jun 17.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.

Transaminases (TAs, EC 2.6.1.X) are a class of promising catalysts for the synthesis of chiral amines. The addition of exogenous expensive cofactor, pyridoxal 5'-phosphate (PLP) leads to the high cost and complicated reaction composition and thus limits the industrial implementation of TAs. In this study, a novel self-sufficient biocatalyst was developed based on transaminase and PLP covalent co-immobilization with the activity recovery of 83.6% and the specific activity of 343.0 U/g catalyst. The self-sufficient BgTA biocatalyst was employed in the continuous biosynthesis of (R)-sitagliptin in a recirculating packed bed reactor (RPBR) for 700 batches reaction without extra addition of PLP. The yield and e.e. of (R)-sitagliptin for each batch were above 90% and 99% respectively. The space-time yield was 40.0 g/(L·h) which was higher than ever reported. This is the first report about the covalent co-immobilization of TA and PLP with high activity and stability, and our work also contributed to the economic flexibility of chiral amine pharmaceutical intermediates biosynthesis.
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http://dx.doi.org/10.1016/j.enzmictec.2019.109362DOI Listing
November 2019
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