Publications by authors named "Xin-Sheng Tang"

8 Publications

  • Page 1 of 1

The complete mitochondrial genome of (Reptilia: Scincidae).

Mitochondrial DNA B Resour 2019 Jul 23;4(2):2727-2728. Epub 2019 Jul 23.

College of Life and Environment Sciences, Huangshan University, Huangshan, P.R. China.

The complete mitochondrial genome (mitogenome) sequence of was sequenced and characterized by next-generation sequencing technology. The total length of mitogenome is 17,027 bp and contains 13 protein-coding genes, 22 tRNA genes, 2 ribosome RNA genes, and 2 non-coding regions (the control region and the putative L-strand replication origin). Most of the genes of are encoded on the H-strand, except for the ND6 subunit gene and 8 tRNA genes which is distributed on the L-strand. Phylogenetic reconstruction suggested that is the sister group of the . within the genus . The complete mitochondrial genome sequence presented here will be useful to study the evolutionary relationships and genetic diversity of .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2019.1644979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706459PMC
July 2019

The complete mitochondrial genome of (Reptilia: Lacertidae).

Mitochondrial DNA B Resour 2019 Jul 10;4(2):2193-2194. Epub 2019 Jul 10.

College of Life and Environment Sciences, Huangshan University, Huangshan, China.

The complete mitochondrial genome sequence of was determined by shotgun sequencing. The total length of mitogenome is 18,304 bp, and contains 13 protein-coding genes, 22 tRNA genes, 2 ribosome RNA genes, and 2 control regions. Most of the genes of . were distributed on the H-strand, except for the ND6 subunit gene and eight tRNA genes which were encoded on the L-strand. The phylogenetic tree of . and 8 other closely related species was reconstructed. The phylogenetic analyses based on these mitogenomes presented here will be useful for further insights on the evolutionary relationships of .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2019.1623123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687526PMC
July 2019

Huangshan population of Chinese Zacco platypus (Teleostei, Cyprinidae) harbors diverse matrilines and high genetic diversity.

Dongwuxue Yanjiu 2016 Mar;37(2):103-9

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan Hubei 430072, China.

Six main mitochondrial DNA (mtDNA) lineages have been described in minnow (Zacco platypus) samples obtained from northern, western and southern China. Perdices et al. (2004) predicted that further sampling of other tributaries might discover more lineages of this species. In this study, we collected 26 Zacco platypus individuals in the Huangshan area of eastern China and determined the cytochrome b (cytb) sequence variations. Combined with reported data in GenBank, we identified ten matrilines (Zacco A-J) in a total of 169 samples, with relatively high molecular divergence found among them. The Huangshan population had the greatest genetic variation among all sampled regions and hosted six of the ten matrilines. Our results highlight the significance of the Huangshan area for the conservation of Zacco platypus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13918/j.issn.2095-8137.2016.2.103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876827PMC
March 2016

Development and characterization of polymorphic microsatellite markers (SSRs) for an endemic plant, Pseudolarix amabilis (Nelson) Rehd. (Pinaceae).

Molecules 2015 Feb 4;20(2):2685-92. Epub 2015 Feb 4.

School of Life Science, Nanjing University, Nanjing 210093, China.

Pseudolarix (Pinaceae) is a vulnerable (sensu IUCN) monotypic genus restricted to southeastern China. To better understand levels of genetic diversity, population structure and gene flow among populations of P. amabilis, we developed five compound SSR markers and ten novel polymorphic expressed sequence tags (EST) derived microsatellites. The results showed that all 15 loci were polymorphic with the number of alleles per locus ranging from two to seven. The expected and observed heterozygosities varied from 0.169 to 0.752, and 0.000 to 1.000, respectively. The inbreeding coefficient ranged from -0.833 to 1.000. These markers will contribute to research on genetic diversity and population genetic structure of P. amabilis, which in turn will contribute to the species conservation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules20022685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272200PMC
February 2015

Characteristic Analysis from Excessive to Deficient Syndromes in Hepatocarcinoma Underlying miRNA Array Data.

Evid Based Complement Alternat Med 2013 8;2013:324636. Epub 2013 Dec 8.

Research Center for TCM Complexity System, Shanghai University of TCM, Shanghai 201203, China.

Traditional Chinese medicine (TCM) treatment is regarded as a safe and effective method for many diseases. In this study, the characteristics among excessive, excessive-deficient, and deficient syndromes of Hepatocellular carcinoma (HCC) were studied using miRNA array data. We first calculated the differentially expressed miRNAs based on random module t-test and classified three TCM syndromes of HCC using SVM method. Then, the weighted miRNA-target networks were constructed for different TCM syndromes using predicted miRNA targets. Subsequently, the prioritized target genes of upexpression network of TCM syndromes were analyzed using DAVID online analysis. The results showed that there are distinctly different hierarchical cluster and network structure of TCM syndromes in HCC, but the excessive-deficient combination syndrome is extrinsically close to deficient syndrome. GO and pathway analysis revealed that the molecular mechanisms of excessive-deficient and deficient syndromes of HCC are more complex than excessive syndrome. Furthermore, although excessive-deficient and deficient syndromes have similar complex mechanisms, excessive-deficient syndrome is more involved than deficient syndrome in development of cancer process. This study suggested that miRNAs might be important mediators involved in the changing process from excessive to deficient syndromes and could be potential molecular markers for the diagnosis of TCM syndromes in HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2013/324636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870617PMC
January 2014

Mitochondrial genome of the Chung-an ground lizard Takydromus sylvaticus (Reptilia: Lacertidae).

Mitochondrial DNA 2014 Aug 24;25(4):319-20. Epub 2013 Jun 24.

College of Life and Environment Sciences, Huangshan University , Huangshan, Anhui , P.R. China .

The Chung-an ground lizard Takydromus sylvaticus is an endemic and long-lost reptile species in China. In this study, its mitochondrial genome (mitogenome) sequence was firstly determined by long PCR and primer walking methods. The genome is 17,838 bp long and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 2 main noncoding regions (the control region and the putative L-strand replication origin). The gene order and contents in the T. sylvaticus mitogenome is identical to that found in typical vertebrates, suggesting that it represents an ancestral arrangement. Within the control region, typical conserved domains and distinct repeat regions were identified.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3109/19401736.2013.800488DOI Listing
August 2014

Bioinformatics analysis of the complete sequences of cytochrome b of Takydromus sylvaticus and modeling the tertiary structure of encoded protein.

Int J Biol Sci 2009 Sep 22;5(6):596-602. Epub 2009 Sep 22.

College of Life and Environment Science, Huangshan University, Huangshan, Anhui, 245021, China.

Cytochrome b (cyt b) gene complete sequences (1143bp) of Takydromus sylvaticus were sequenced. In order to clarify the phylogenetic position of the Takydromus sylvaticus, we investigated the phylogeny of 15 Takydromus spp. distributed in East-Asia by Maximum Parsimony (MP), Bayesian Inference (BI), and Maximum Likelihood (ML) methods using DNA fragments of cyt b genes. The results supported that the Platyplacopus merged into Takydromus and negated the validity of Platyplacopus. Furthermore, the prediction of tertiary structures of cyt b exhibited the CD loop region contain two short helices forming a hairpin arrangement, namely cd1 and cd2. Thermostability analysis shows that the CD-loop region is unstable thermodynamically and may provide mobility to amino acids located at the heme, and might provide high flexibility to the top of ISP (iron-sulfur protein) and the cavity region of Qo binding site. It suggested that the two short helices of CD loop region of cyt b was a dominating portion for ISP binding site.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijbs.5.596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2748471PMC
September 2009

Description of male Takydromus sylvaticus (Squamata: Lacertidae) from China, with notes on sexual dimorphism and a revision of the morphological diagnosis of the species.

Zoolog Sci 2007 May;24(5):496-503

Department of Biology, Huangshan University, Huangshan, Anhui, PR China.

The Chungan ground lizard, Takydromus sylvaticus, long known only from five females collected in Fujian Province of China, was recently found in Anhui Province of the country. The newly collected specimens included the first male specimens (n=7), in which the tail bases were stouter than in conspecific females. Ventral scales in these males were invariably keeled, whereas in females those forming the four median rows were smooth. Females had more dorsal scales (47-48) than males (42-43) in the median longitudinal row between axilla and groin. Takydromus sylvaticus differed from the closely related T. dorsalis in having more dorsal scales in the median longitudinal row between axilla and groin (42-48 versus 32-40, respectively), more rows of large dorsal scales between the hind legs (10 versus 7-8, respectively), shorter hind legs, two distinct white longitudinal stripes on each side of the body, and no black stripe in the preocular and postocular regions. The number of transverse rows of ventral scales between collar and femoral pores in T. sylvaticus (26-28) was larger, but with a slight overlap, than that in T. dorsalis (22-26). Ecological observations on T. sylvaticus were also documented.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2108/zsj.24.496DOI Listing
May 2007
-->