Publications by authors named "Xin-Jian Zhang"

11 Publications

  • Page 1 of 1

History of IUD utilization and the risk of preterm birth: a cohort study.

Arch Gynecol Obstet 2021 Jul 30. Epub 2021 Jul 30.

Dongguan Maternal and Child Health Care Hospital, No.99 Zhenxing Road, Dongcheng District, Dongguan City, 523112, Guangdong Province, China.

Objective: To explore whether a history of IUD use could increase the risk of subsequent preterm birth.

Methods: We performed a cohort study of 24,496 multipara aged 19-48 years in Dongguan City. Each subject was followed up for 1 year, and 12,508 women obtained pregnancy outcomes. They were divided into 2 groups: 2130 subjects with IUD use history (exposure group), and 10,378 subjects without IUD use history (control group). The exposure group will remove the IUD before pregnancy. The primary outcomes were preterm birth (less than 37 weeks of gestation) and early preterm birth (less than 34 weeks of gestation). We used log-binomial regression to estimate adjusted risk ratios (aRR) of preterm birth and early preterm birth for women with a history of IUD. According to the different adjusted baseline data, three regression models were established, and the propensity matching score method was also used to verify the stability of the results.

Results: The delivery rate of women with IUD history was 51.24%, and that of women without IUD was 51.03% ( = 0.063, P = 0.802). Six hundred and eighty-five women had preterm birth (5.48%, 95% CI 5.08-5.88) and 133 women had early preterm birth (1.06%, 95% CI 0.83-1.24). Compared with the control group, the incidence of preterm birth and early preterm birth in the exposure group were significantly lower. The results are stable in all four models. Subgroup analysis also supported the result. This study also found that the longer the women used IUD before pregnancy, the younger the age of first using IUD, and the shorter the time from condom removal to pregnancy, the lower the incidence of premature birth.

Conclusion: The women with a history of IUD use are less likely to have premature birth after the IUD is removed. More prospective studies are needed to confirm it.
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http://dx.doi.org/10.1007/s00404-021-06158-9DOI Listing
July 2021

(Brassicaceae), a new species from China, with a re-evaluation of the complex.

PhytoKeys 2021 4;178:179-191. Epub 2021 Jun 4.

CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China Kunming Institute of Botany, Chinese Academy of Sciences Kunming China.

, from Qinghai and Tibet (China), is described and illustrated. Morphological and molecular data indicate that is closely related to , from which it is easily distinguished by the densely hirsute (vs. glabrous or sparsely pubescent), elliptic to ovate-lanceolate (vs. suborbicular, oblong, or lanceolate) fruits with undulate (vs. straight) margins. A re-evaluation of the widely distributed and related taxa is also provided.
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http://dx.doi.org/10.3897/phytokeys.178.62922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195944PMC
June 2021

Development and characterization of a panel of cross-reactive monoclonal antibodies generated using H1N1 influenza virus.

Immunobiology 2015 Aug 14;220(8):941-6. Epub 2015 Feb 14.

Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States.

To characterize the antigenic epitopes of the hemagglutinin (HA) protein of H1N1 influenza virus, a panel consisting of 84 clones of murine monoclonal antibodies (mAbs) were generated using the HA proteins from the 2009 pandemic H1N1 vaccine lysate and the seasonal influenza H1N1(A1) vaccines. Thirty-three (39%) of the 84 mAbs were found to be strain-specific, and 6 (7%) of the 84 mAbs were subtype-specific. Twenty (24%) of the 84 mAbs recognized the common HA epitopes shared by 2009 pandemic H1N1, seasonal A1 (H1N1), and A3 (H3N2) influenza viruses. Twenty-five of the 84 clones recognized the common HA epitopes shared by the 2009 pandemic H1N1, seasonal A1 (H1N1) and A3 (H3N2) human influenza viruses, and H5N1 and H9N2 avian influenza viruses. We found that of the 16 (19%) clones of the 84 mAbs panel that were cross-reactive with human respiratory pathogens, 15 were made using the HA of the seasonal A1 (H1N1) virus and 1 was made using the HA of the 2009 pandemic H1N1 influenza virus. Immunohistochemical analysis of the tissue microarray (TMA) showed that 4 of the 84 mAb clones cross-reacted with human tissue (brain and pancreas). Our results indicated that the influenza virus HA antigenic epitopes not only induce type-, subtype-, and strain-specific monoclonal antibodies against influenza A virus but also cross-reactive monoclonal antibodies against human tissues. Further investigations of these cross-reactive (heterophilic) epitopes may significantly improve our understanding of viral antigenic variation, epidemics, pathophysiologic mechanisms, and adverse effects of influenza vaccines.
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http://dx.doi.org/10.1016/j.imbio.2015.02.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124281PMC
August 2015

[Energy balance and evapotranspiration in broad-leaved Korean pine forest in Changbai Mountains].

Ying Yong Sheng Tai Xue Bao 2011 Mar;22(3):607-13

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

Based on the continuous measurements of an open-path eddy covariance system, this paper analyzed the characteristics of energy balance components and evapotranspiration in a broad-leaved Korean pine forest in Changbai Mountains in 2008, as well as the differences of energy balance components and evapotranspiration between growth season and dormant season. For the test forest, the year-round energy balance closure was 72%, being at a medium level, compared to the other studies in the Fluxnet community. The energy balance components had significant differences in their diurnal and seasonal variations. In growth season, turbulent energy exchange was dominated by upward latent heat flux, accounting for 66% of available energy; while in dormant season, the turbulent energy exchange was dominated by upward sensible heat flux, accounting for 63% of available energy. The accumulated annual evapotranspiration in the study site in 2008 was 484.7 mm, occupying 87% of the precipitation at the same time period (558.9 mm), which demonstrated that evapotranspiration was the main water loss item in temperate forests of northern China.
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March 2011

Cajanol inhibits the growth of Escherichia coli and Staphylococcus aureus by acting on membrane and DNA damage.

Planta Med 2011 Jan 27;77(2):158-63. Epub 2010 Aug 27.

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, PR China.

In the present study, the mechanism of antibacterial activity of cajanol extracted from the roots of Cajanus cajan (L.) Millsp. towards Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was investigated. The antibacterial activity of cajanol was evaluated towards six bacterial strains (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa) by the broth microdilution method. It showed strong antibacterial activity towards all bacteria tested with minimal inhibition concentration (MIC) values ranging from 98.90 µM to 197.8 µM. Cajanol-induced death rates in the most sensitive strains ( E.COLI, 96.55 % and S. AUREUS, 97.25 %) were analyzed by flow cytometry. Furthermore, the activity of cajanol on the membranes of E. coli and S. aureus was investigated by using lecithin, phosphate groups, and fluorescence microscopy. Cajanol-induced DNA damage was observed by agarose gel electrophoresis. In summary, cajanol inhibited E. coli only by DNA damage, whereas S. aureus was inhibited by affecting both, the lecithin and phosphate groups on the cellular membrane and DNA. The present study shows that cajanol possesses antibacterial activity in vitro towards both gram-negative and gram-positive bacteria and therefore may be a promising candidate as an antibacterial agent for the therapy of microbial infections.
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http://dx.doi.org/10.1055/s-0030-1250146DOI Listing
January 2011

[Accuracy of perioperative cardiac preload monitoring by global end-diastolic volume and intrathoracic blood volume in orthotopic liver transplantation].

Nan Fang Yi Ke Da Xue Xue Bao 2010 Jul;30(7):1577-9

Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.

Objective: To investigate the clinical value of global end-diastolic volume (GEDV) and intrathoracic blood volume (ITBV) in perioperative monitoring of the cardiac preload in patients undergoing orthotopic liver transplantations (OLT).

Methods: Eight ASA III or IV patients aged 42-50 years undergoing OLT without venovenous bypass under general anesthesia were enrolled in this study. Before the induction, a thermodilution femoral artery catheter was inserted into the femoral artery under local anesthesia and connected to a PiCCOplus system to monitor ITBV and GEDV. A CCO catheter was inserted into the right internal jugular vein to monitor the pulmonary artery obstruction pressure (PAOP), central venous press (CVP) and stroke volume (SVPAC). Anesthesia was induced with a combination of midazolam (0.1 mg/kg), propofol (1 mg/kg) and fentanyl (3 microg/kg). Pipecuronium (0.1 mg/kg) was given to facilitate naso-endotracheal intubation. Before anesthesia (T0) and at 10 min before the anhepatic phase (T1), 10 min after anhepatic phase (T2), 10 min after neohepatic phase (T3) and at the end of surgery (T4), all the TPTD and CCO parameters were measured by injecting 10 ml cold saline solution (below 8 degrees celsius;) via the distal port of the central venous catheter.

Results: ITBV and GEDV at T2 were significantly lower than those at T0, T1, T3 and T4 (P<0.05). SVPAC at T2 was dramatically decreased compared with that at T0 and T1 (P<0.05). The changes in the pressure preload parameters of the pulmonary artery catheter (PAOP and CVP) did not correlate to the changes in SVPAC, whereas the changes in the volume preload parameters (ITBV and GEDV) of the TPTD was significantly correlated to the changes in SVPAC (P<0.01). PAOP and CVP did not correlate to the changes in ITBV and GEDV.

Conclusion: ITBV and GEDV are more reliable than PAOP and CVP in perioperative monitoring of the cardiac preload in patients undergoing OLT.
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July 2010

[Effect of different doses of recombinant adenovirus mediated hypoxia-inducible factor-1alpha on angiogenesis in ischemic lower hind limb of rabbits].

Nan Fang Yi Ke Da Xue Xue Bao 2010 Feb;30(2):210-3

Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To investigate the effect of recombinant adenovirus-mediated hypoxia-inducible factor-1alpha (Ad-HIF-1alpha) at different doses on angiogenesis in a rabbit model of hind limb ischemia.

Methods: Left hind limb ischemia was induced in 45 Zealand white rabbits by ligation of the left femoral artery. The rabbits were randomly divided into 5 groups (n=9) to receive intramuscular injections of 0.5 ml saline, 2x10(10) PFU empty vector (Ad-null), or different doses of Ad-HIF-1alpha (2x10(9), 2x10(10) or 2x10(11) PFU) immediately after the operation. On the 7th day after the operation, real-time PCR was used to detect the expression of HIF-1alpha mRNA in the skeletal muscles. Immediately and on the 14th and 28th days after the operation, contrast enhanced ultrasound (CEU) was used to observe the blood perfusion of the hind limb. On the 28th day postoperatively, immunohistochemistry for CD31 was performed to evaluate the microvascular density (MVD).

Results: Real-time PCR showed that Ad-HIF-1alpha significantly increased the expression of HIF-1alpha mRNA (P<0.01) in a dose-dependent manner as compared with that in the saline and Ad-null groups (P<0.01). CEU revealed greater blood perfusion in the hind limb of rabbits in association with increased dose of Ad-HIF-1alpha (P<0.05 or P<0.01); similar changes in the MVD was observed following Ad-HIF-1alpha injections as shown by immunohistochemistry (P<0.05 or P<0.01). No significant differences were found either in the blood perfusion or MVD between saline and Ad-null groups (P>0.05).

Conclusion: Ad-HIF-1alpha can dose-dependently promote the angiogenesis in the ischemic limb of rabbits.
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February 2010

[Effect of propofol at different effect-site concentrations on approximate entropy of transient evoked otoacoustic emission signals in adults].

Nan Fang Yi Ke Da Xue Xue Bao 2009 Jan;29(1):94-6

Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To study the effect of propofol at different effect-site concentrations on approximate entropy (ApEn) of transient evoked otoacoustic emission (TEOAE) signals in adults and investigate the possibility of using ApEn for monitoring anesthesia depth.

Methods: Fifteen ASA class I or II patients (aged 18-49 years with normal hearing) undergoing elective surgery under general anesthesia were enrolled in this study. Anesthesia was maintained with target-controlled infusion of propofol. With the effect-site concentrations of 1, 2, 3 and 4 microg/ml, TEOAE signals were monitored and recorded before and after anesthesia. ApEn of TEOAE in 4 frequency ranges (0-2, 1-3, 2.5-4.5, and 4-6 kHz) were calculated using MATLAB software.

Results: The ApEn of TEOAE in different frequency ranges showed no significant differences at the same effect-site concentration of propofol, or at different effect-site concentrations in the same frequency range (P>0.05).

Conclusion: Anesthesia with propofol at different effect-site concentrations does not obviously affect ApEn of TEOAE signals in adults, and ApEn can not be used as the indicator for evaluating the depth of anesthesia.
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January 2009

Altered inotropic reactivity in diabetic rabbit right ventricular myocardium.

Can J Physiol Pharmacol 2004 Oct;82(10):903-10

Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock 79430-6592, USA.

Alloxan monohydrate was used to induce diabetes in rabbits, which were maintained for a 3-month period with or without daily insulin replacement along with age-matched controls. Isolated right ventricular myocardial strips were used to generate dose-response curves to isoproterenol, forskolin, and Bay K 8644. Basal developed force was significantly elevated in diabetic ventricular strips. While isoproterenol acted as a full inotropic agonist, diabetic preparations revealed a consistent but insignificant decrease in the maximum developed force. While both sensitivity to isoproterenol and beta-adrenoceptor density were decreased in preparations from diabetic rabbits, there was no associated increase in circulating plasma catecholamines. In contrast, forskolin and Bay K 8644 were partial agonists in control preparations but full inotropic agonists in diabetic preparations, demonstrating significant increases in maximum developed force. This hyperresponsiveness was not associated with altered calcium channel density. Finally, insulin replacement reduced or prevented all diabetic-related changes. These data indicate that the hyperresponsiveness to forskolin and Bay K 8644 represents an altered utilization of intracellular calcium in the diabetic rabbit, converting them into full agonists similar to isoproterenol. The decrease in sensitivity to isoproterenol correlated with a decrease in beta-adrenoceptor density but not elevated circulating catecholamines as previously observed in diabetic rats.
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http://dx.doi.org/10.1139/y04-101DOI Listing
October 2004

Hypoglycemic effects of taurine in the alloxan-treated rabbit, a model for type 1 diabetes.

Adv Exp Med Biol 2003 ;526:97-104

Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.

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http://dx.doi.org/10.1007/978-1-4615-0077-3_13DOI Listing
February 2004

High-level Expression and Characterization of the Fusion Protein Consisting of Diphtheria Toxin and Human Interleukin 6.

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1998 ;30(2):169-173

Institute of Virology, the Chinese Academy of Preventive Medicine, Beijing 100052, China.

Diphtheria toxin is a single chain exotoxin of 535 amino acids, secreted from beta Corynebacteriophage diphtheriae. Eukaryotic cells, especially tumor cells are very sensitive to DT. Just one or two molecules of DT can kill a cell. On the surface of some tumor cells, such as human myeloma, heptoma, etc, IL-6 receptor has been demonstrated to be expressed at a very-high level. Selective cytotoxicity mediated by IL-6 receptor could be useful in the targeting therapy of these tumors. Based on this strategy, a hybrid protein consisting of DT and IL-6 was constructed, expressed and characterized. IL-6 cDNA was first modified for constructing the fusion protein of DT/IL-6 and the receptor binding domain of DTDNA was replaced by IL-6 cDNA to obtain the expression vector pdeltaDT/TL-6. After induction by IPTG, the fusion protein was expressed successfully, accounting for 20% of the total bacterial protein. The results of SDS-PAGE and Western blot showed that there was a band of about 64 kD. After preliminary purification, the IL-6 receptor competitive binding test and the cytotoxic activity assay of the deltaDT/TL-6 showed that the fusion protein possessed significant cytotoxic activity to U266 cells; while the cells expressing IL-6 receptors at a medium-level was resistant it to a certain degree.
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January 1998
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