Publications by authors named "Xin Zhong"

330 Publications

Exosomal Vaccine Loading T Cell Epitope Peptides of SARS-CoV-2 Induces Robust CD8 T Cell Response in HLA-A Transgenic Mice.

Int J Nanomedicine 2022 29;17:3325-3341. Epub 2022 Jul 29.

Institute of Nephrology, Zhongda Hospital, Medical School of Southeast University, Nanjing, 210009, People's Republic of China.

Purpose: Current vaccines for the SARS-CoV-2 virus mainly induce neutralizing antibodies but overlook the T cell responses. This study aims to generate an exosomal vaccine carrying T cell epitope peptides of SARS-CoV-2 for the induction of CD8 T cell response.

Methods: Thirty-one peptides presented by HLA-A0201 molecule were conjugated to the DMPE-PEG-NHS molecules, and mixed with DSPE-PEG to form the peptide-PEG-lipid micelles, then fused with exosomes to generate the exosomal vaccine, followed by purification using size-exclusion chromatography and validation by Western blotting, liquid nuclear magnetic resonance (NMR) test and transmission electron microscopy. Furthermore, the exosomal vaccine was mixed with Poly (I:C) adjuvant and subcutaneously administered for three times into the hybrid mice of HLA-A0201/DR1 transgenic mice with wild-type mice. Then, the epitope-specific T cell responses were detected by ex vivo ELISPOT assay and intracellular cytokine staining.

Results: The exosomal vaccine was purified from the Peak 2 fraction of FPLC and injected into the hybrid mice for three times. The IFN-γ spot forming units and the frequencies of IFN-γ/CD8 T cells were 10-82-fold and 13-65-fold, respectively, higher in the exosomal vaccine group compared to the Poly (I:C) control group, without visible organ toxicity. In comparison with the peptides cocktail vaccine generated in our recent work, the exosomal vaccine induced significantly stronger T cell response.

Conclusion: Exosomal vaccine loading T cell epitope peptides of SARS-CoV-2 virus was initially generated without pre-modification for both peptides and exosomes, and elicited robust CD8 T cell response in HLA-A transgenic mice.
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http://dx.doi.org/10.2147/IJN.S367494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346304PMC
August 2022

Plastid Phylogenomics and Plastome Evolution of Nandinoideae (Berberidaceae).

Front Plant Sci 2022 30;13:913011. Epub 2022 Jun 30.

Laboratory of Systematic & Evolutionary Botany and Biodiversity, College of Life Sciences, Zhejiang University, Hangzhou, China.

Subfamily Nandinoideae Heintze (Berberidaceae), comprising four genera and 19 species, is disjunctively distributed in eastern North America vs. Eurasia (eastern Asia, Central Asia, Middle East, and southeastern Europe), and represents an ideal taxon to explore plastid phylogenomics and plastome evolution in Berberidaceae. Many species of this subfamily have been listed as national or international rare and endangered plants. In this study, we sequenced and assembled 20 complete plastomes, representing three genera and 13 species of Nandinoideae. Together with six plastomes from GenBank, a total of 26 plastomes, representing all four genera and 16 species of Nandinoideae, were used for comparative genomic and phylogenomic analyses. These plastomes showed significant differences in overall size (156,626-161,406 bp), which is mainly due to the expansion in inverted repeat (IR) regions and/or insertion/deletion (indel) events in intergenic spacer (IGS) regions. A 75-bp deletion in the F gene occurred in and when compared with and . We found a severe truncation at the 5' end of 1 in three plastomes, and a premature termination of 1 in . Our phylogenomic results support the topology of {, [, (, )]}. Within the core genus , we identified from northeastern Asia (Clade A) as the earliest diverging species, followed by from eastern China (Clade B), while the rest species clustered into the two sister clades (C and D). Clade C included three species from West Tianshan (, , ). Clade D consisted of from northern Central Asia, plus one species from the Caucasus Mountains () and three from southeastern Europe (, , ). Overall, we identified 21 highly variable plastome regions, including two coding genes (22, 1) and 19 intergenic spacer (IGS) regions, all with nucleotide diversity () values > 0.02. These molecular markers should serve as powerful tools (including DNA barcodes) for future phylogenetic, phylogeographic and conservation genetic studies.
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http://dx.doi.org/10.3389/fpls.2022.913011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302238PMC
June 2022

Impact of Subintimal Plaque Modification on Reattempted Chronic Total Occlusions Percutaneous Coronary Intervention.

JACC Cardiovasc Interv 2022 07;15(14):1427-1437

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai, China; Institutes of Biomedical Sciences, Fudan University, Shanghai, China. Electronic address:

Background: Predictors of success in reattempted chronic total occlusion (CTO) percutaneous coronary intervention (PCI) procedures remain obscure, mainly owing to the lack of consecutive angiograms and procedural records of initial attempts in the same cohort.

Objectives: This study sought to investigate the factors predicting the success of reattempted CTO PCI procedures.

Methods: A total of 208 consecutive patients who underwent a failed CTO PCI attempt and received reattempted procedure at the same cardiac center were retrospectively analyzed. Predictors of the success of reattempted procedures were evaluated.

Results: The overall technical success rate of reattempted CTO PCI procedures was 71.2%. Subintimal plaque modification (SPM) was implemented in 35 (16.8%) procedures in initial attempts. The reattempted technical success rate was 93.3% in cases in which SPM with guidewire (GW) crossing was achieved in the initial attempt; however, the success rate was 55.0% for procedures involving SPM without GW crossing. SPM with GW crossing (OR: 11.21; 95% CI: 1.31-96.16; P = 0.028), referral to high-volume operators (OR: 2.38; 95% CI: 1.14-4.98; P = 0.021), and a bidirectional approach (OR: 2.31; 95% CI: 1.12-4.79; P = 0.024) were positive independent predictors of technical success in the subsequent reattempt. The time interval for reattempt (per 90-day increment) was negatively correlated with the technical success of the reattempted procedures (OR: 0.85; 95% CI: 0.73-0.98; P = 0.030).

Conclusions: This study identified independent predictors of success in reattempted CTO PCI procedures. SPM with GW crossing achieved in the initial attempt is associated with a higher success rate in the subsequent reattempt.
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http://dx.doi.org/10.1016/j.jcin.2022.06.015DOI Listing
July 2022

Spread and Molecular Characteristics of Carrying -Like Genes from Farms in China.

Microbiol Spectr 2022 Jul 19:e0054522. Epub 2022 Jul 19.

College of Animal Science and Technology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Universitygrid.256609.e, Nanning, China.

In this study, we aimed to investigate the occurrence and molecular characteristics of fosfomycin-resistant isolates from pig, chicken and pigeon farms in Guangxi Province of China. A total of 200 fosfomycin-resistant strains were obtained from food animals and their surrounding environments, with the , , and genes being detected in 26% (52/200), 10% (20/200), and 5% (10/200), respectively. Surprisingly, three -producing E. coli isolates were found to be concomitant with . Most of the -like-gene-positive isolates were multidrug-resistant strains and consistently possessed , , and genes. Only was successfully transferred to the recipient strains, and the 29 -carrying transconjugants exhibited high-level resistance to fosfomycin (MIC ≥ 512 μg/mL). Multilocus sequence typing (MLST) combined with enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analyses indicated that or genes were spread by horizontal transfer as well as via clonal transmission between E. coli. We used the PCR mapping method to explore the genetic contexts of -like genes, and two representative strains (fEc.1 and fEcg99-1) were fully sequenced. Six different genetic structures surrounding were detected and one infrequent context was discovered among the conjugable -positive E. coli isolates. The five genetic environments of were identified and found to be highly similar to the partial sequence of transposon Tn. Furthermore, whole-genome sequencing (WGS) results showed that was colocalized with , , , (A), , and a number of virulence-related factors on the same chromosomes of strains, and various insertion sequences (IS/IS) were detected upstream or downstream of . The phylogenetic analysis revealed that both - and -carrying E. coli ST602 and -carrying E. coli ST2599 were closely related to E. coli isolates from humans, which may indicate that they pose a threat to human health. Here, we report the widespread and complex genetic environments of -like genes in animal-derived strains in China. The gene was identified in this study and was found to confer resistance to fosfomycin. The high prevalence of -like genes in farms indicates that food animals serve as a potential reservoir for the resistance genes. This study also discovered that fosfomycin resistance genes were always associated with mobile elements, which would accelerate the transmission of -like genes in strains. Importantly, E. coli ST602 and ST2599 carrying or from food animals had high similarity to E. coli isolates from humans, suggesting that -like genes can be transmitted to humans through the food chain, thus posing a serious threat to public health. Therefore, the prevalence of -like genes isolated from animals should be further monitored.
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http://dx.doi.org/10.1128/spectrum.00545-22DOI Listing
July 2022

Offset-decoupled deformable convolution for efficient crowd counting.

Sci Rep 2022 Jul 18;12(1):12229. Epub 2022 Jul 18.

Department of Educational Technology, Ocean University of China, Qingdao, 266100, China.

Crowd counting is considered a challenging issue in computer vision. One of the most critical challenges in crowd counting is considering the impact of scale variations. Compared with other methods, better performance is achieved with CNN-based methods. However, given the limit of fixed geometric structures, the head-scale features are not completely obtained. Deformable convolution with additional offsets is widely used in the fields of image classification and pattern recognition, as it can successfully exploit the potential of spatial information. However, owing to the randomly generated parameters of offsets in network initialization, the sampling points of the deformable convolution are disorderly stacked, weakening the effectiveness of feature extraction. To handle the invalid learning of offsets and the inefficient utilization of deformable convolution, an offset-decoupled deformable convolution (ODConv) is proposed in this paper. It can completely obtain information within the effective region of sampling points, leading to better performance. In extensive experiments, average MAE of 62.3, 8.3, 91.9, and 159.3 are achieved using our method on the ShanghaiTech A, ShanghaiTech B, UCF-QNRF, and UCF_CC_50 datasets, respectively, outperforming the state-of-the-art methods and validating the effectiveness of the proposed ODConv.
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http://dx.doi.org/10.1038/s41598-022-16415-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293988PMC
July 2022

Prediction of Above-Room-Temperature Superconductivity in Lanthanide/Actinide Extreme Superhydrides.

J Am Chem Soc 2022 Jul 12;144(29):13394-13400. Epub 2022 Jul 12.

State Key Laboratory of Superhard Materials and International Center for Computational Method & Software, College of Physics, Jilin University, Changchun 130012, China.

Achieving room-temperature superconductivity has been an enduring scientific pursuit driven by broad fundamental interest and enticing potential applications. The recent discovery of high-pressure clathrate superhydride LaH with superconducting critical temperatures () of 250-260 K made it tantalizingly close to realizing this long-sought goal. Here, we report a remarkable finding based on an advanced crystal structure search method of a new class of extremely hydrogen-rich clathrate superhydride MH (M: rare-earth/actinide atom) stoichiometric compounds stabilized at an experimentally accessible pressure of 350 GPa. These compounds are predicted to host up to 330 K, which is well above room temperature. The bonding and electronic properties of these MH clathrate superhydrides closely resemble those of atomic metallic hydrogen, giving rise to the highest hitherto found in a thermodynamically stable hydride compound. An in-depth study of these extreme superhydrides offers insights for elucidating phonon-mediated superconductivity above room temperature in hydrogen-rich and other low-Z materials.
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http://dx.doi.org/10.1021/jacs.2c05834DOI Listing
July 2022

Impact of intracoronary reteplase during primary percutaneous coronary intervention on infarct size in large anterior myocardial infarction: rationale and design of the RECOVER II trial.

Cardiovasc Diagn Ther 2022 Jun;12(3):352-359

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Thrombus embolization and microvascular obstruction during percutaneous coronary intervention (PCI) in ST-segment elevation myocardial infarction (STEMI) is commonly detected, which causes inadequate myocardial perfusion and elevated infarct size. An approach with low-dose intracoronary fibrinolytic treatment for reducing distal embolization and improving myocardial reperfusion in high-risk STEMI cases remains controversial.

Methods: The RECOVER II study represents a multicenter, randomized, double-blind, parallel-group trial assessing low-dose adjunctive intracoronary reteplase during primary PCI in individuals with large anterior myocardial infarction and thrombus determined by angiography. The trial will enroll 306 cases who present within 12 h following STEMI for proximal or mid left anterior descending artery occlusion undergoing primary PCI. Cases will be randomized to receive a bolus intracoronary reteplase at 9 mg or 18 mg . placebo. The drug will be delivered over 2 minutes proximal to culprit lesions with an intracoronary catheter early after wire-crossing and before thrombus aspiration or balloon dilation.

Results: The primary outcome will be infarct size assessed by late gadolinium-enhanced magnetic resonance imaging (MRI) (% of left ventricular mass) on day 7 after enrollment. Secondary outcomes will include the amount of microvascular obstruction and myocardial salvage index examined via MRI on day 7, angiographic measures of reperfusion [Thrombolysis in Myocardial Infarction (TIMI) coronary flow grade, TIMI frames count and myocardial blush grade], incidence of complete ST-segment resolution at 2 hours after reperfusion, area under the curve for troponin T, and rates of major adverse cardiovascular events at 30 days.

Conclusions: RECOVER II will determine whether the addition of low-dose intracoronary reteplase early after wire-crossing as an adjunct to reperfusion treatment reduces infarct size in individuals with large anterior myocardial infarction.

Trial Registration: ClinicalTrials.gov Identifier: NCT04571580.
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http://dx.doi.org/10.21037/cdt-21-756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253174PMC
June 2022

The diversity of elaborate petals in Isopyreae (Ranunculaceae): a special focus on nectary structure.

Protoplasma 2022 Jun 27. Epub 2022 Jun 27.

Key Laboratory of Medicinal Plant Resource and Natural Pharmaceutical Chemistry of Ministry of Education, Shaanxi Normal University, Xi'an, 710062, China.

Elaborate petals are highly diverse in morphology, structure, and epidermal differentiation and play a key role in attracting pollinators. There have been few studies on the elaborate structure of petals in the tribe Isopyreae (Ranunculaceae). Seven genera in Isopyreae (Aquilegia, Semiaquilegia, Urophysa, Isopyrum, Paraquilegia, Dichocarpum, and Leptopyrum) have petals that vary in morphology, and two genera (Enemion and Thalictrum) have no petals. The petals of nine species belonged to 7 genera in the tribe were studied to reveal their nectary structure, epidermal micromorphology and ancestral traits. The petal nectaries of Isopyreae examined in this study were located at the tip of spurs (Aquilegia yabeana and A. rockii), or the bottom of shallow sacs (Semiaquilegia adoxoides, Urophysa henryi, Isopyrum manshuricum, and Paraquilegia microphylla), a cup-shaped structure (Dichocarpum fargesii) and a bilabiate structure (Leptopyrum fumarioides). The petal nectary of eight species in Isopyreae (except A. ecalcarata) was composed of secretory epidermis, nectary parenchyma, and vascular tissues, and some sieve tubes reached the secretory parenchyma cells. Among the eight species with nectaries examined in the present study, A. yabeana had the most developed nectaries, with 10-15 layers of secretory parenchyma cells. The epidermal cells of mature petals of the nine species were divided into 11 types. Among these 11 types, there were two types of secretory cells and two types of trichomes. Aquilegia yabeana and A. rockii had the highest number of cell types (eight types), and I. manshuricum and L. fumarioides had the lowest number of cell types (three types). Aquilegia ecalcarata had no secretory cells, and the papillose conical polygonal secretory cells of D. fargesii were different from those of the other seven species with nectaries. Trichomes were found only in Aquilegia, Semiaquilegia, Urophysa, and Paraquilegia. The ancestral mode of nectar presentation in Isopyreae was petals with hidden nectar (70.58%). The different modes of nectar presentation in petals may reflect adaptations to different pollinators in Isopyreae.
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http://dx.doi.org/10.1007/s00709-022-01787-6DOI Listing
June 2022

Germacrone induces caspase-3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis.

Exp Ther Med 2022 Jul 20;24(1):456. Epub 2022 May 20.

Department of Liver Disease, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, Guangdong 518033, P.R. China.

Liver cancer is a highly lethal malignancy. Despite considerable efforts made in recent years, the prognosis of patients with liver cancer remains poor. (known as Ezhu in Chinese) is widely prescribed in traditional Chinese medicine. Germacrone (GM) is a sesquiterpene constituent derived from the essential oil of Ezhu, and exerts anti-carcinogenic effects by inducing apoptosis in various cancer cells. The present study investigated the potential mechanism of GM in HepG2 cells. Cell Counting Kit-8, colony-formation and lactate dehydrogenase-release assays, as well as cell death assays using flow cytometry, were performed to evaluate HepG2 cell proliferation following GM treatment. HepG2 cells were transfected with caspase-3 small interfering RNA and then treated with GM. Caspase-3 expression levels were determined by reverse transcription-quantitative PCR and western blotting. The present study showed that GM inhibited the growth of HepG2 cells and induced the proteolytic cleavage of caspase 3, with concomitant cleavage of gasdermin E (GSDME), by markedly increasing the production of reactive oxygen species (ROS). This led to caspase 3-dependent cleavage of GSDME, thereby promoting pyroptosis in HepG2 cells. However, these changes were rescued by ROS scavengers, such as N-acetylcysteine. Furthermore, GM inhibited tumor growth by promoting the cleavage of caspase 3 and GSDME in HepG2 cell xenograft models. These results indicated that GM induced GSDME-dependent pyroptosis through caspase 3 activation, at least in part, by damaging the mitochondria and enhancing ROS production, thereby supporting the possible development of GM as a candidate for the prevention and treatment of liver cancer.
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http://dx.doi.org/10.3892/etm.2022.11383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204551PMC
July 2022

Aerobic Exercise Inhibited P2X7 Purinergic Receptors to Improve Cardiac Remodeling in Mice With Type 2 Diabetes.

Front Physiol 2022 31;13:828020. Epub 2022 May 31.

Department of Physical Education, Wenzhou Medical University, Wenzhou, China.

Diabetic cardiomyopathy (DCM), the main complication of diabetes mellitus, presents as cardiac dysfunction by ventricular remodeling. In addition, the inhibition of P2X7 purinergic receptors (P2X7R) alleviates cardiac fibrosis and apoptosis in Type 1 diabetes. However, whether exercise training improves cardiac remodeling by regulating P2X7R remains unknown. Db/db mice spontaneously induced with type 2 diabetes and high-fat diet (HFD) and mice with streptozotocin (STZ)-induced type 2 diabetes mice were treated by 12-week treadmill training. Cardiac functions were observed by two-dimensional echocardiography. Hematoxylin-eosin staining, Sirius red staining and transmission electron microscopy were respectively used to detect cardiac morphology, fibrosis and mitochondria. In addition, real-time polymerase chain reaction and Western Blot were used to detect mRNA and protein levels. Studying the hearts of db/db mice and STZ-induced mice, we found that collagen deposition and the number of disordered cells significantly increased compared with the control group. However, exercise markedly reversed these changes, and the same tendency was observed in the expression of MMP9, COL-I, and TGF-β, which indicated cardiac fibrotic and hypertrophic markers, including ANP and MyHC expression. In addition, the increased Caspase-3 level and the ratio of Bax/Bcl2 were reduced by exercise training, and similar results were observed in the TUNEL test. Notably, the expression of P2X7R was greatly upregulated in the hearts of db/db mice and HFD + STZ-induced DM mice and downregulated by aerobic exercise. Moreover, we indicated that P2X7R knock out significantly reduced the collagen deposition and disordered cells in the DM group. Furthermore, the apoptosis levels and TUNEL analysis were greatly inhibited by exercise or in the P2X7R group in DM. We found significant differences between the P2X7R + DM + EX group and DM + EX group in myocardial tissue apoptosis and fibrosis, in which the former is significantly milder. Moreover, compared with the P2X7R + DM group, the P2X7R + DM + EX group represented a lower level of cardiac fibrosis. The expression levels of TGF-β at the protein level and TGF-β and ANP at the genetic level were evidently decreased in the P2X7R + DM + EX group. Aerobic exercise reversed cardiac remodeling in diabetic mice at least partly through inhibiting P2X7R expression in cardiomyocytes.
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http://dx.doi.org/10.3389/fphys.2022.828020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197582PMC
May 2022

The International Penile Prosthesis Implant Consensus Forum: clinical recommendations and surgical principles on the inflatable 3-piece penile prosthesis implant.

Nat Rev Urol 2022 Jun 16. Epub 2022 Jun 16.

University of Western Ontario, London, Ontario, Canada.

Despite significant scientific advances in the modern three-piece inflatable penile prosthesis implant surgery, it is not without surgical risks and can carry additional cosmetic and psychosocial consequences in poorly selected and consented individuals. To address this problem, an international group of key opinion leaders and high-volume prosthetic surgeons reviewed the current guidelines and clinical evidence, discussed their experiences, and formed a consensus regarding inflatable penile prosthesis surgery. The findings of this consensus panel were presented at the 17th biennial Asia Pacific Society of Sexual Medicine scientific meeting. The experts concluded that proper patient selection, informed consent and strict adherence to safe surgical principles are important to optimize clinical outcomes. Furthermore, most intraoperative complications, if recognized, can be addressed intraoperatively to enable placement of the device at the time of initial surgery. Men with significant corporal fibrosis due to Peyronie's disease, prior prosthesis explantation and priapism, and men who have undergone construction of a neophallus, as well as men who receive concurrent continence surgery, are complex cases requiring additional care and advanced techniques to obtain optimal surgical outcomes. Variability in patient care - in terms of postoperative antibiotic use, pain management, scrotal care, and cycling of the penile prosthesis implant - must be reduced to enable optimization and assessment of outcomes across patient groups.
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http://dx.doi.org/10.1038/s41585-022-00607-zDOI Listing
June 2022

High-speed and efficient removal of uranium (VI) from aqueous solution by hydroxyapatite-modified ordered mesoporous carbon (CMK-3).

Environ Sci Pollut Res Int 2022 Jun 15. Epub 2022 Jun 15.

School of Life Science, Shaoxing University, Huancheng West Road 508, Shaoxing, 312000, People's Republic of China.

In recent years, the synthesis and application of green, cost-effective, and sustainable materials for uranium (VI) removal was significant to environmental protection. The ordered mesoporous carbon (CMK-3) supported different mass of hydroxyapatite materials ([email protected]) were facilely synthesized via hydrothermal method. The resultant materials were characterized by XRD, FT-IR, BET, SEM, TEM mapping, and XPS, and implemented for immobilizing U(VI). Not only the specific surface area of HAP (7.01 m/g) was increased by the loading on CMK-3 (818.37 m/g), but also the adsorption capacity of CMK-3 was increased by HAP modification. Impressively, [email protected] exhibited highly adsorption capacity of U(VI) with the increase of HAP deposition and was capable of achieving fast reaction. Therein to, the specific surface area of [email protected](2:1) was 253.68 m/g, as well as the adsorption capacity was up to 1072 mg/g (fitted by Langmuir isotherm, at pH=3.0, 298 K) and the adsorption process was completed in 30 min (followed by pseudo-second-order kinetic). The adsorption mechanisms of U(VI) on [email protected] involved electrostatic forces, ionic interactions, and chemical complexation. This work offered new avenues to address the limitations of cost and less secondary pollution for the removal of U(IV) from wastewater.
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http://dx.doi.org/10.1007/s11356-022-21351-2DOI Listing
June 2022

Genetic Evidence Supporting a Causal Role of Snoring in Erectile Dysfunction.

Front Endocrinol (Lausanne) 2022 25;13:896369. Epub 2022 May 25.

Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.

Background: The association between snoring and erectile dysfunction (ED) is inconsistent in multiple observational studies. To clarify the causal association of snoring on ED, we performed this two-sample Mendelian randomization study.

Materials And Methods: The single nucleotide polymorphisms (SNPs) associated with snoring were retrieved from the UK biobank cohort with 314,449 participants (117,812 cases and 196,637 controls). The summary statistics of ED were obtained from the European ancestry with 223,805 subjects (6,175 cases and 217,630 controls). Single-variable Mendelian randomization (MR) and multivariable MR were used to assess the causal relationship between snoring and ED.

Results: Snoring increases the risk of ED (Odds ratio [OR] = 3.45, 95% confidence interval [CI] = 1.68 - 7.09, < 0.001) in the inverse variance weighting estimator. In sensitivity analyses, the ORs for the weighted median, MR robust adjusted profile score, and MR Pleiotropy Residual Sum and Outlier approach, MR-Egger, and maximum likelihood method are 5.70 (95% CI = 1.19 - 27.21, < 0.05), 3.14 (95% CI = 1.01 - 9.72, < 0.05), 3.11 (95% CI = 1.63 - 5.91, < 0.01), 1.23 (95% CI = 0.01 - 679.73, > 0.05), and 3.59 (95% CI = 1.07 - 12.00, < 0.05), respectively. No heterogeneity and pleiotropy are observed ( for MR-Egger intercept = 0.748; for global test = 0.997; for Cochran's Q statistics > 0.05). After adjusting for total cholesterol, triglyceride, low-density lipoprotein, and cigarette consumption, the ORs for ED are 5.75 (95% CI = 1.80 - 18.34, < 0.01), 4.16 (95% CI = 1.10 - 15.81, < 0.05), 5.50 (95% CI = 1.62 - 18.69, < 0.01), and 2.74 (95% CI = 1.06 - 7.10, < 0.05), respectively.

Conclusion: This study provides genetic evidence supporting the causal role of snoring in ED.
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http://dx.doi.org/10.3389/fendo.2022.896369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174907PMC
May 2022

A high TSH level is associated with diabetic macular edema: a cross-sectional study of patients with type 2 diabetes mellitus.

Endocr Connect 2022 Jul 29;11(7). Epub 2022 Jun 29.

Beijing Diabetes Institute, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Aims: In this study, we determined the association between thyroid-stimulating hormone (TSH) and diabetic macular edema (DME) by assessing the prevalence and risk factors for DME in type 2 diabetes mellitus (T2DM) patients with different thyroid dysfunctions.

Methods: This was a retrospective cross-sectional study including 1003 euthyroid and 92 subclinical hypothyroidism (SCH) T2DM patients. DME status was detected by optical coherence tomography (OCT). The association between TSH and DME and the impact of TSH on DME were analyzed.

Results: The DME prevalence was 28.3% in the SCH patients and 14.0% in the euthyroid population. The serum FT4 (P = 0.001) and FT3 (P < 0.001) levels were significantly higher in the non-DME group than in the DME group, and the TSH level (P < 0.001) was significantly lower. Four subgroups (G1-G4) were divided by TSH level, and the chi-square test indicated that even in the normal range, the TSH level was positively related to DME prevalence (P = 0.001). Subgroup data indicated that the association between TSH and DME detected by OCT (P = 0.001) was stronger than the correlation between TSH and diabetic retinopathy detected by digital retinal photographs (P = 0.027). The logistic regression model confirmed that elevated TSH was an independent risk factor for DME. The odds ratio was 1.53 (P = 0.02).

Conclusions: A high TSH level was an independent risk factor for DME. More attention should be given to the TSH level in T2DM patients due to its relationship with diabetic complications.
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http://dx.doi.org/10.1530/EC-22-0122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254322PMC
July 2022

Enhanced Photocatalytic Hydrogen Production Activity by Constructing a Robust Organic-Inorganic Hybrid Material Based Fulvalene and TiO.

Nanomaterials (Basel) 2022 Jun 3;12(11). Epub 2022 Jun 3.

Hainan Provincial Key Laboratory of Fine Chemicals, College of Chemical Engineering and Technology, Hainan University, Haikou 570228, China.

A novel redox-active organic-inorganic hybrid material (denoted as HTTFTB-TiO) based on tetrathiafulvalene derivatives and titanium dioxide with a micro/mesoporous nanomaterial structure has been synthesized via a facile sol-gel method. In this study, tetrathiafulvalene-3,4,5,6-tetrakis(4-benzoic acid) (HTTFTB) is an ideal electron-rich organic material and has been introduced into TiO for promoting photocatalytic H production under visible light irradiation. Notably, the optimized composites demonstrate remarkably enhanced photocatalytic H evolution performance with a maximum H evolution rate of 1452 μmol g h, which is much higher than the prototypical counterparts, the common dye-sensitized sample (denoted as HTTFTB-5.0/TiO) (390.8 μmol g h) and pure TiO (18.87 μmol g h). Moreover, the composites perform with excellent stability even after being used for seven time cycles. A series of characterizations of the morphological structure, the photoelectric physics performance and the photocatalytic activity of the hybrid reveal that the donor-acceptor structural HTTFTB and TiO have been combined robustly by covalent titanium ester during the synthesis process, which improves the stability of the hybrid nanomaterials, extends visible-light adsorption range and stimulates the separation of photogenerated charges. This work provides new insight for regulating precisely the structure of the fulvalene-based composite at the molecule level and enhances our in-depth fundamental understanding of the photocatalytic mechanism.
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http://dx.doi.org/10.3390/nano12111918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182102PMC
June 2022

Detection and Quantification of Fusarium oxysporum f. sp. niveum Race 1 in Plants and Soil by Real-time PCR.

Plant Pathol J 2022 Jun 1;38(3):229-238. Epub 2022 Jun 1.

College of Horticulture, Hebei Agricultural University, Baoding 071001, China.

Fusarium wilt caused by Fusarium oxysporum f. sp. niveum (Fon) is the most serious soil-borne disease in the world and has become the main limiting factor of watermelon production. Reliable and quick detection and quantification of Fon are essential in the early stages of infection for control of watermelon Fusarium wilt. Traditional detection and identification tests are laborious and cannot efficiently quantify Fon isolates. In this work, a real-time polymerase chain reaction (PCR) assay has been described to accurately identify and quantify Fon in watermelon plants and soil. The FONRT-18 specific primer set which was designed based on identified specific sequence amplified a specific 172 bp band from Fon and no amplification from the other formae speciales of Fusarium oxysporum tested. The detection limits with primers were 1.26 pg/µl genomic DNA of Fon, 0.2 pg/ng total plant DNA in inoculated plant, and 50 conidia/g soil. The PCR assay could also evaluate the relationships between the disease index and Fon DNA quantity in watermelon plants and soil. The assay was further used to estimate the Fon content in soil after disinfection with CaCN2. The real-time PCR method is rapid, accurate and reliable for monitoring and quantification analysis of Fon in watermelon plants and soil. It can be applied to the study of disease diagnosis, plant-pathogen interactions, and effective management.
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http://dx.doi.org/10.5423/PPJ.OA.03.2022.0039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343908PMC
June 2022

Late contralateral recurrence of retinal detachment in incontinentia pigmenti: A case report.

World J Clin Cases 2022 May;10(13):4171-4176

Department of Ophthalmology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China.

Background: Incontinentia pigmenti (IP) is a rare X-linked genetic disease. It mainly manifests as skin lesions and causes problems in the eyes, teeth, bones, and central nervous system. Of the various ocular manifestations, the most severe with difficult recovery is retinal detachment (RD). Here, we report an unusual case of bilateral asymmetrical RD.

Case Summary: We present the case of an 11-year-old Chinese girl with IP who complained of sudden blurring of vision in the left eye. At that time, she had been blind in her right eye for 4 years. RD with traction was observed in both eyes. A massive retinal proliferative membrane, exudation, and hemorrhage were seen in the left eye. We performed vitrectomy in her left eye. Her visual acuity recovered to 20/50, and her retina had flattened within 2 d after surgery. During the 3-mo follow-up, we performed retinal laser treatment of the non-perfused retinal area in her left eye. Eventually, her visual acuity returned to 20/32, and no new retinal abnormalities developed.

Conclusion: In patients with IP with fundal abnormalities in one eye, it is important to focus on the rate of fundal change in the other eye. RD in its early stages can be effectively treated with timely vitrectomy and laser photocoagulation.
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http://dx.doi.org/10.12998/wjcc.v10.i13.4171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131234PMC
May 2022

Association of Circulating Apolipoprotein AI Levels in Patients With Alzheimer's Disease: A Systematic Review and Meta-Analysis.

Front Aging Neurosci 2022 18;14:899175. Epub 2022 May 18.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.

With the development of medicine, our research on Alzheimer's disease (AD) has been further deepened, but the mechanism of its occurrence and development has not been fully revealed, and there is currently no effective treatment method. Several studies have shown that apolipoprotein AI (ApoA-I) can affect the occurrence and development of Alzheimer's disease by binding to amyloid β (Aβ). However, the association between circulating levels of ApoA-I and AD remains controversial. We conducted a meta-analysis of 18 studies published between 1992 and 2017 to determine whether the ApoA-I levels in the blood and cerebrospinal fluid (CSF) are abnormal in AD. Literatures were searched in PubMed, EMBASE and Web of Science databases without language limitations. A pooled subject sample including 1,077 AD patients and 1,271 healthy controls (HCs) was available to assess circulating ApoA-I levels; 747 AD patients and 680 HCs were included for ApoA-I levels in serum; 246 AD patients and 456 HCs were included for ApoA-I levels in plasma; 201 AD patients and 447 HCs were included for ApoA-I levels in CSF. It was found that serum and plasma levels of ApoA-I were significantly reduced in AD patients compared with HCs {[standardized mean difference (SMD) = -1.16; 95% confidence interval (CI) (-1.72, -0.59); = 0.000] and [SMD = -1.13; 95% CI (-2.05, -0.21); = 0.016]}. Patients with AD showed a tendency toward higher CSF ApoA-I levels compared with HCs, although this difference was non-significant [SMD = 0.20; 95% CI (-0.16, 0.56); = 0.273]. In addition, when we analyzed the ApoA-I levels of serum and plasma together, the circulating ApoA-I levels in AD patients was significantly lower [SMD = -1.15; 95% CI (-1.63, -0.66); = 0.000]. These results indicate that ApoA-I deficiency may be a risk factor of AD, and ApoA-I has the potential to serve as a biomarker for AD and provide experimental evidence for diagnosis of AD. PROSPERO, identifier: 325961.
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http://dx.doi.org/10.3389/fnagi.2022.899175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157647PMC
May 2022

Non-muscle-invasive bladder cancer with metastasis to cervical lymph nodes without local recurrence or progression: A case report.

Asian J Surg 2022 May 26. Epub 2022 May 26.

Department of Urology, West China Hospital, Sichuan University, Chengdu, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.05.077DOI Listing
May 2022

Proton density fat fraction measurements of rotator cuff muscles: Accuracy, repeatability, and reproducibility across readers and scanners.

Magn Reson Imaging 2022 Oct 24;92:260-267. Epub 2022 May 24.

Department of Radiology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China. Electronic address:

Purpose: To determine the accuracy, repeatability, and reproducibility of magnetic resonance imaging-based proton density fat fraction (MRI-PDFF) measurements of rotator cuff muscles between two readers and three different scanners.

Methods: Twenty-seven volunteers underwent serial shoulder MRI examinations of both left and right sides on one 1.5-T MRI scanner and two 3.0-T MRI scanners. Two independent readers measured muscular PDFF of the supraspinatus, infraspinatus/teres minor muscle, and subscapularis. MR spectroscopy-based proton density fat fraction (MRS-PDFF) was regarded as the reference standard for assessing accuracy. A "coffee break" examination method was used to test the repeatability of each scanner. Bland-Altman plots, Pearson correlation, and linear regression analysis were used to assess bias and linearity. The Wilcoxon signed-rank test and Friedman test were applied to evaluate repeatability and reproducibility.

Results: MRI-PDFF measurements indicated strong linearity (R = 0.749) and small bias (-0.18%) in comparison with the MRS-PDFF measurements. A very strong positive Pearson correlation (r = 0.955-0.986) between the PDFF estimates of the two repeat scans indicated excellent repeatability. The PDFF measurements showed high reproducibility, with a strong positive Pearson correlation (r = 0.668-0.698) and a small mean bias (-0.04 to -0.10%) across different scanners.

Conclusion: MRI-PDFF measurements of rotator cuff muscles were highly accurate, repeatable, and reproducible across different readers and scanners, leading us to the conclusion that PDFF can be a reliable and robust quantitative imaging biomarker for longitudinal or multi-center studies.
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http://dx.doi.org/10.1016/j.mri.2022.05.013DOI Listing
October 2022

Comparison Between Linear Stapler and Circular Stapler After Laparoscopic-Assisted Distal Gastrectomy in Patients With Gastric Cancer.

Front Surg 2022 6;9:858236. Epub 2022 May 6.

Department of Gastrointestinal Surgery, General Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background And Aim: To evaluate the safety and efficacy of laparoscopy distal gastrectomy using a linear stapler compared with a circular stapler in patients with gastric cancer.

Methods: We retrospectively reviewed 173 patients who underwent laparoscopic distal gastrectomy for gastric cancer at a single center from January 2018 to December 2020. Patients were categorized into the linear stapler group and the circular stapler group. General data, intraoperative and postoperative outcomes, postoperative pathological results, postoperative complications, and postoperative follow-up in the two groups were compared and analyzed.

Results: The operation time (208.76 ± 32.92 vs. 226.69 ± 26.92 min, < 0.05), anastomosis time (71.87 ± 9.50 vs. 90.56 ± 3.18 min, < 0.05), time to first flatus (68.60 ± 25.96 vs. 76.16 ± 21.05 h, < 0.05), time to the first sip of water (3.66 ± 0.61 vs. 4.07 ± 0.77 days, < 0.05), and time to the first liquid diet (4.43 ± 1.02 vs. 5.03 ± 1.70 days, < 0.05) were significantly shorter in the linear stapler group. In addition, the highest postoperative body temperature within 3 days (37.4 ± 0.61 vs. 37.7 ± 0.61, < 0.05) after the operation, white blood cell count (WBC) on the 3rd day (9.07 ± 2.52 vs. 10.01 ± 2.98 × 10∧9/L, < 0.05), and average gastric tube drainage within 3 days (36.65 ± 24.57 vs. 52.61 ± 37 ml, < 0.05) were also significantly lower in the linear stapler group.

Conclusions: Both circular and linear staplers are safe and feasible for gastrointestinal reconstruction in laparoscopic distal gastrectomy. In contrast, a linear stapler has advantages over a circular stapler in shortening operation time and accelerating the postoperative recovery of patients.
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http://dx.doi.org/10.3389/fsurg.2022.858236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120601PMC
May 2022

Increasing obstructive sleep apnea risk is associated with hearing impairment in middle-aged Chinese men-A cross-sectional study.

PLoS One 2022 20;17(5):e0268412. Epub 2022 May 20.

Physical Examination Department, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Objective: Midlife males with obstructive sleep apnea (OSA) bear a high risk for cardiovascular diseases. However, the association of OSA and hearing impairment is controversial. Our objective was to observe the incidence of hearing loss in middle-aged males with different risks for OSA.

Methods: 794 men aged 40-65 who participated in health examination and pure tone hearing screening between January and June 2021 were recruited in the study. Medical history was collected. Height, weight and blood pressure were tested, and biochemical test including blood lipids and blood glucose was performed. According to the STOP-BANG score, the observed subjects were divided into low, intermediate and high groups for OSA risk. Hearing impairment was defined as failure in responding to any pure tone of 25 dB HL in any ear at the frequencies: 4 kHz for high frequency range and 0.5k, 1k, 2 kHz for low/medium frequency range. The incidence of hearing loss in those groups was compared after adjusting the cardiovascular risk factors.

Results: The incidence of hearing impairment in the groups of intermediate, high, and intermediate/high risk for OSA (46.9%, 45.2%, 46.3%, respectively) were higher than that in the group of low risk for OSA (33.3%, P<0.001). After adjusting cardiovascular risk factors, the risk of hearing impairment in the group of high risk for OSA is 1.64 times of the group of low risk for OSA (95%CI: 1.02-2.69, P<0.05). The risk of hearing impairment at high frequency(4kHz) in the group of intermediate/high risk for OSA is 1.43 times of the group of low-risk for OSA (95%CI: 1.00-2.06, P<0.05).

Conclusion: The risk of hearing impairment in midlife men with high, intermediate/high risk for OSA is significantly increased, especially at high frequency of 4 kHz.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0268412PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122213PMC
May 2022

Platelet Activating Factor Receptor Exaggerates Microglia-Mediated Microenvironment by IL10-STAT3 Signaling: A Novel Potential Biomarker and Target for Diagnosis and Treatment of Alzheimer's Disease.

Front Aging Neurosci 2022 28;14:856628. Epub 2022 Apr 28.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.

Background: Early diagnosis and effective intervention are the keys to delaying the progression of Alzheimer's Disease (AD). Therefore, we aimed to identify new biomarkers for the early diagnosis of AD through bioinformatic analysis and elucidate the possible underlying mechanisms.

Methods And Results: GSE1297, GSE63063, and GSE110226 datasets from the GEO database were used to screen the highly differentially expressed genes. We identified a potential biomarker, Platelet activating factor receptor (PTAFR), significantly upregulated in the brain tissue, peripheral blood, and cerebrospinal fluid of AD patients. Furthermore, PTAFR levels in the plasma and brain tissues of APP/PS1 mice were significantly elevated. Simultaneously, PTAFR could mediate the inflammatory responses to exaggerate the microenvironment, particularly mediated by the microglia through the IL10-STAT3 pathway. In addition, PTAFR was a putative target of anti-AD compounds, including EGCG, donepezil, curcumin, memantine, and Huperzine A.

Conclusion: PTAFR was a potential biomarker for early AD diagnosis and treatment which correlated with the microglia-mediated microenvironment. It is an important putative target for the development of a novel strategy for clinical treatment and drug discovery for AD.
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http://dx.doi.org/10.3389/fnagi.2022.856628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096237PMC
April 2022

Time Course of Coagulo-Fibrinolytic Derangements During Acclimatization to High Altitude in Rabbits and a Preliminary Study on the Possible Mechanisms.

High Alt Med Biol 2022 May 11. Epub 2022 May 11.

State Key Laboratory of Trauma, Burn and Combined Injury, Department for Combat Casualty Care Training, Training Base for Army Health Care, Army Medical University, Chongqing, China.

Zhong, Xin, Zhao Ye, Xiaolin Zhou, Renqing Jiang, Yijun Jia, Wenqiong Du, Haoyang Yang, Lin Zhang, Bai Lu, and Zhaowen Zong. Time course of coagulo-fibrinolytic derangements during acclimatization to high altitude in rabbits and a preliminary study on the possible mechanisms. . 00:000-000, 2022. Conflicting data exist regarding changes in the coagulation system during acclimatization to high altitude (HA), which makes the prevention of thromboembolic events difficult. The present study aimed at observing the dynamic changes in the coagulo-fibrinolysis system during acclimatization to HA and at exploring the possible mechanisms. Twenty rabbits of both sexes were randomly divided into two groups, including group A rabbits (healthy plain controls) and group B rabbits (acutely exposed to HA). A traditional coagulation test, thromboelastography analysis, and full blood cell count were used to assess the coagulo-fibrinolytic changes at different time points. Plasma was collected to examine the levels of relevant biomarkers. Six hours and 1 day after acute exposure to HA, the coagulo-fibrinolytic system demonstrated a hypercoagulable state. Further, 3 days after exposure to HA, group B rabbits showed hypocoagulability, increased fibrinolysis, and lower clot firmness and 7 days after exposure to HA, delayed coagulation, decreased fibrinolysis, and increased clot firmness were observed. Subsequently, 14, 21, and 28 days after exposure to HA, we found increased clot firmness. Increased platelet counts and concentrations of fibrinogen and plasminogen activator inhibitor-1 contributed to this change. The coagulo-fibrinolytic derangements during acclimatization to HA in rabbits demonstrated a dynamic pattern.
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http://dx.doi.org/10.1089/ham.2021.0120DOI Listing
May 2022

A randomized multicenter trial to evaluate early invasive strategy for patients with acute ST-segment elevation myocardial infarction presenting 24-48 hours from symptom onset: Protocol of the RESCUE-MI study.

Am Heart J 2022 Sep 5;251:54-60. Epub 2022 May 5.

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China. Electronic address:

Background: For ST-segment elevation myocardial infarction (STEMI) patients presenting 24 to 48 hours from symptom onset, whether early invasive strategy should be performed still remains controversial.

Methods: This is a prospective, open-label, multicenter, investigator initiated, randomized controlled trial (NCT04962178) to evaluate the efficacy of early invasive strategy for STEMI patients within 24 to 48 hours of symptom onset. A total of 366 patients will be included from 10 hospitals in mainland China. They will be randomly (1:1) divided into 2 groups: the early invasive strategy group (primary percutaneous coronary intervention, PPCI) and conservative strategy group (optimal medical therapy with primary PCI not performed). All patients will be followed for 1 month. The primary end point is myocardial infarction size on cardiac magnetic resonance (CMR). The secondary end points are as follows: (1) major adverse cardiovascular events (MACE), which is defined as a composite of cardiac death, recurrent myocardial infarction, ischemic driven target vessel revascularization and stroke; (2) other CMR end points, including microvascular obstruction, intramyocardial hemorrhage, myocardial area at risk, left ventricular ejection fraction, left ventricular end diastolic volume and left ventricular end systolic volume.

Discussion: This study is designed to evaluate the efficacy of early invasive strategy for STEMI patients within 24 to 48 hours of symptom onset and will add more evidence for clinical practice.

Trial Registration: ClinicalTrials.gov Identifier: NCT04962178. Registered on July 14, 2021.
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http://dx.doi.org/10.1016/j.ahj.2022.05.002DOI Listing
September 2022

Ox-LDL-mediated ILF3 overexpression in gastric cancer progression by activating the PI3K/AKT/mTOR signaling pathway.

Aging (Albany NY) 2022 05 4;14(9):3887-3909. Epub 2022 May 4.

Department of Gastrointestinal Surgery, General Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.

Background: This study aimed to investigate the relationship of dyslipidemia and interleukin-enhancer binding factor 3 (ILF3) in gastric cancer, and provide insights into the potential application of statins as an agent to prevent and treat gastric cancer.

Methods: The expression levels of ILF3 in gastric cancer were examined with publicly available datasets such as TCGA, and western blotting and immunohistochemistry were performed to determine the expression of ILF3 in clinical specimens. The effects of ox-LDL on expression of ILF3 were further verified with western blot analyses. RNA sequencing, Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and Gene Set Enrichment Analysis (GSEA) pathway analyses were performed to reveal the potential downstream signaling pathway targets of ILF3. The effects of statins and ILF3 on PI3K/AKT/mTOR signaling pathway, cell proliferation, cell cycle, migration and invasion of gastric cancer cells were investigated with Edu assay, flow cytometry and transwell assay.

Results: Immunohistochemistry and western blot demonstrated that the positive expression rates of ILF3 in gastric cancer tissues were higher than adjacent mucosa tissues. The ox-LDL promoted the expression of ILF3 in a time-concentration-dependent manner. ILF3 promoted the proliferation, cell cycle, migration and invasion by activating the PI3K/AKT/mTOR signaling pathway. Statins inhibited the proliferation, cell cycle, migration and invasion of gastric cancer by inhibiting the expression of ILF3.

Conclusions: These findings demonstrate that ox-LDL promotes ILF3 overexpression to regulate gastric cancer progression by activating the PI3K/AKT/mTOR signaling pathway. Statins inhibits the expression of ILF3, which might be a new targeted therapy for gastric cancer.
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http://dx.doi.org/10.18632/aging.204051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134943PMC
May 2022

A Case Report of Intravitreal Dexamethasone Implant with Exudative Retinal Detachment for Ocular Toxocariasis Treatment.

Korean J Parasitol 2022 Apr 20;60(2):133-137. Epub 2022 Apr 20.

Department of Ophthalmology, The First Affiliated Hospital of Guangxi Medical University, Nanning 513200, Guangxi Zhuang Autonomous Region, China.

Toxocariasis is one of the most common geohelminth infections in several parts of the world. We describe a rare case of ocular toxocariasis with secondary exudative retinal detachment treated with albendazole and an intravitreal dexamethasone implant. A 13-year-old boy with counting finger vision was diagnosed with retinal vasculitis and exudative retinal detachment in his right eye. Fundoscopic examination revealed retinal hemorrhage, retinal vasculitis, and exudative retinal detachment. Serological test using serum and intraocular aqueous humor were positive for anti-Toxocara specific IgG antibodies. He received repeated doses of intravitreal dexamethasone implants combined with oral albendazole. A sequential follow-up optical coherence tomography revealed that the retina was successfully reattached. His visual acuity subsequently improved to 20/400.
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http://dx.doi.org/10.3347/kjp.2022.60.2.133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058278PMC
April 2022

Association between hypertension and nonalcoholic fatty liver disease: a cross-sectional and meta-analysis study.

J Hum Hypertens 2022 Apr 11. Epub 2022 Apr 11.

Department of Preventive Medicine, Medical College, Yanbian University, 977 Gongyuan Street, Yanji, 133000, Jilin, China.

The association between hypertension and nonalcoholic fatty liver disease (NAFLD) is not completely understood. This study aimed to investigate the association between hypertension and hepatic ultrasound examination-diagnosed positive NAFLD in healthy people; to conduct a comprehensive meta-analysis combining the results of previous studies; to explore whether hypertension was a risk factor for NAFLD. This study included 2049 adults (male: 870 and female: 1179), aged ≥20 years, whose anthropometric parameters were measured to analyze the risk of hypertension on NAFLD. We also collected data from 11 cross-sectional studies relevant to this topic using PubMed, Embase, Web of Science, CNKI, Wanfang, and CQVIP from beginning till 31 August 2020 and combined it with our data for a meta-analysis to explore whether hypertension was a risk factor for NAFLD. After adjusting for confounding factors, the odds of NAFLD in hypertensive subjects was 1.473 (95%CI: 1.119-1.938). After combining with 10 selected studies, 42711 participants were enrolled in meta-analysis. Hypertension was a risk factor for NAFLD (Z = 13.46, P < 0.001); the odds of NAFLD in hypertensive subjects was 1.43 (95%CI: 1.36-1.51). The results were consistent with the results of the meta-analysis. Further studies are required to confirm these results.
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http://dx.doi.org/10.1038/s41371-022-00686-wDOI Listing
April 2022

Study on the application of preoperative three-dimensional CT angiography of perigastric arteries in laparoscopic radical gastrectomy.

Sci Rep 2022 04 11;12(1):6026. Epub 2022 Apr 11.

Department of Gastrointestinal Surgery, Qilu Hospital of Shandong University, No. 107, Wenhuaxi Road, Jinan, 250012, Shandong Province, China.

To investigate the clinical value and significance of preoperative three-dimensional computerized tomography angiography (CTA) in laparoscopic radical gastrectomy for gastric cancer. The clinical data were analyzed retrospectively from 214 gastric cancer patients. We grouped according to whether to perform CTA, and we compared and analyzed the difference of the data between the two groups. The perigastric arteries were classified according to CTA images of patients in the CTA group. The celiac trunk was classified according to Adachi classification: Type I (118/125, 94.4%), Type II (3/125, 2.4%), Type III (0/125, 0%), Type IV (1/125, 0.8%), Type V (2/125, 1.6%), Type VI (1/125, 0.8%). Hepatic artery classification was performed according to Hiatt classification: Type I (102/125, 81.6%), Type II (9/125, 7.2%), Type III (6/125, 4.8%), Type IV (2/125, 1.6%), Type V (3/125, 2.4%), Type VI (0, 0%), Others (3/125, 2.4%). And this study combined vascular anatomy and surgical risk to establish a new splenic artery classification model. In comparison, the operation time, first exhaust time, and estimated blood loss in the CTA group were significantly lower than those in the non-CTA group. In addition, the blood loss in the CTA group combined with ICG (Indocyanine Green) labeled fluorescence laparoscopy was significantly less than that in the group without ICG labeled. Preoperative CTA could objectively evaluate patients' vascular route and variation and then help us avoid or decrease the risk of vascular injury and bleeding. When combined with ICG labeled fluorescence laparoscopy, it could further reduce the risk of iatrogenic injury during the operation and improve postoperative recovery.
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http://dx.doi.org/10.1038/s41598-022-09584-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9001703PMC
April 2022

Ultrasound-targeted microbubble destruction (UTMD)-mediated miR-150-5p attenuates oxygen and glucose deprivation-induced cardiomyocyte injury by inhibiting TTC5 expression.

Mol Biol Rep 2022 Jul 31;49(7):6041-6052. Epub 2022 Mar 31.

Department of Cardiovascular, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, 61, Jiefang West Road, Furong District, Changsha City, 410002, Hunan, China.

Background: Cardiomyocyte injury is a typical feature in cardiovascular diseases. Changes in cardiomyocytes strongly affect the progression of cardiovascular diseases. This work aimed to investigate the biological function and potential mechanism of action of miR-150-5p in cardiomyocytes.

Methods And Results: A myocardial ischemia (MI) injury rat model was constructed to detect miR-150-5p and tetratricopeptide repeat domain 5 (TTC5) expression during heart ischemia injury. Primary cardiomyocytes were isolated for in vitro study. CCK-8 assays were used to detect cardiomyocyte viability. Western blots were used to detect TTC5 and P53 expression. qPCR was utilized to measure RNA expression of miR-150-5p and TTC5. The TUNEL assay was used to determine cell apoptosis. ELISA was used to determine cytokine (TNF-α, IL-1β, IL-6, and IL-8) levels in heart tissues and cell culture supernatants. A dual-luciferase reporter assay was carried out to verify the binding ability between miR-150-5p and TTC5. Oxygen-glucose deprivation (OGD) treatment significantly inhibited cell viability. Ultrasound-targeted microbubble destruction (UTMD)-mediated uptake of miR-150-5p inverted these results. Additionally, UTMD-mediated uptake of miR-150-5p retarded the effects of OGD treatment on cell apoptosis. Besides, UTMD-mediated uptake of miR-150-5p counteracted the effects of OGD treatment on the inflammatory response by regulating cytokine (TNF-α, IL-1β, IL-6, and IL-8) levels. For the mechanism of the protective effect on the heart, we predicted and confirmed that miR-150-5p bound to TTC5 and inhibited TTC5 expression.

Conclusions: UTMD-mediated uptake of miR-150-5p attenuated OGD-induced primary cardiomyocyte injury by inhibiting TTC5 expression. This discovery contributes toward further understanding the progression of primary cardiomyocyte injury.
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http://dx.doi.org/10.1007/s11033-022-07392-3DOI Listing
July 2022
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