Publications by authors named "Xin Zhang"

5,424 Publications

  • Page 1 of 1

Total IgE variability is associated with future asthma exacerbations: a one-year prospective cohort study.

J Allergy Clin Immunol Pract 2021 May 12. Epub 2021 May 12.

Airway Physiology and Imaging Group and the Woolcock Emphysema Centre, The Woolcock Institute of Medical Research, Sydney, NSW, Australia; Discipline of Medical Sciences, School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia.

Background: Few prospective studies have investigated the relationship between IgE variability and risk of asthma exacerbations (AEs).

Objective: To explore the relationship between IgE variability and AEs.

Methods: Recruited patients with stable asthma underwent two serum total IgE tests within a month (at screening [baseline IgE] and one-month) to obtain coefficient of variation (CV) of base-10 log-transformed IgE. Patients with IgE CV were divided into IgE CV-high and IgE CV-low cohorts based on the CV's median and followed up within a 12-month period during which, the association between IgE variability and AEs was explored using a negative binomial regression model.

Results: IgE CV levels obtained from 340 patients classified the patients into two groups (n=170 for the IgE CV-high and IgE CV-low groups, respectively) based on the serum total IgE CV median of 2.12% (Q1, Q3: 0.98%, 3.91%). IgE CV-high patients exhibited worse asthma control and lung function, more marked airway inflammation, and received more intensive medication use compared to IgE CV-low patients. IgE CV-high patients exhibited increased rates of moderate-to-severe (adjusted rate ratio [RR]=2.88, 95% CI=[1.65, 5.03], P<0.001) and severe (RR=2.16, 95% CI=[1.08, 4.32], P=0.029) AEs during the follow-up year compared to IgE CV-low patients. Furthermore, sputum IL-6 partially mediated the associations between IgE CV with moderate-to-severe and severe AEs.

Conclusions: Variability in total serum IgE levels is an easily obtained and practical measure for predicting AEs. Future studies are needed to investigate whether IgE variability can be used to guide precision medicine in asthma.
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http://dx.doi.org/10.1016/j.jaip.2021.04.065DOI Listing
May 2021

Percutaneous cholecystostomy versus emergency cholecystectomy for the treatment of acute calculous cholecystitis in high-risk surgical patients: a meta-analysis and systematic review.

Updates Surg 2021 May 15. Epub 2021 May 15.

Department of Gastrointestinal Surgery, Changzheng Hospital, Naval Medical University, Shanghai, China.

The present meta-analysis was performed to compare the efficacy and safety of percutaneous cholecystostomy (PC) versus emergency cholecystectomy (EC) for the treatment of acute calculous cholecystitis (ACC) in high-risk surgical patients. Literature searches for eligible studies were performed using MEDLINE, EMBASE and the Cochrane Library. Quality assessment was conducted in each study. Meta-analyses were performed to demonstrate the pooled effects of relative risk (RR) with 95% confidence intervals (CI). A total of 8960 patients from 6 studies were finally included. PC resulted in increased risks of mortality (RR = 2.87; CI = 1.33-6.18; p = 0.007) and readmission rate (RR = 4.70; CI = 3.30-6.70; p < 0.00001) as compared with EC. No significant difference was detected between PC and EC in terms of morbidity, severe complication rate or hospitalization length. Moreover, PC was associated with significantly higher risks of mortality (RR = 7.47; CI = 1.88-29.72; p = 0.004), morbidity (RR = 3.71; 95% CI = 1.78-7.75; p = 0.0005), readmission rate (RR = 7.91; CI = 3.80-16.49; p < 0.00001), and hospitalization length (WMD = 6.92; CI = 5.89-7.95; p < 0.00001) when directly compared with laparoscopic cholecystectomy (LC). Therefore, EC is superior to PC for the treatment of ACC in high-risk surgical patients, and LC is the preferred surgical strategy.
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http://dx.doi.org/10.1007/s13304-021-01081-9DOI Listing
May 2021

Programmable two-dimensional nanocrystals assembled from POSS-containing peptoids as efficient artificial light-harvesting systems.

Sci Adv 2021 May 14;7(20). Epub 2021 May 14.

Physical Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.

Inspired by the formation of hierarchically structured natural biominerals (e.g., bone and tooth), various sequence-defined polymers have been synthesized and exploited for design and synthesis of functional hybrid materials. Here, we synthesized a series of organic-inorganic hybrid peptoids by using polyhedral oligomeric silsesquioxane (POSS) nanoclusters as side chains at a variety of backbone locations. We further demonstrated the use of these hybrid peptoids as sequence-defined building blocks to assemble a new class of programmable two-dimensional (2D) nanocrystals. They are highly stable and exhibit an enhanced mechanical property and electron scattering due to the incorporated POSS nanoclusters. By varying peptoid side-chain chemistry, we further demonstrated the precise displacement of a large variety of function groups within these 2D nanocrystals and developed a highly efficient aqueous light-harvesting system for live cell imaging. Because these 2D nanocrystals are biocompatible and highly programmable, we expect that they offer unique opportunities for applications.
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http://dx.doi.org/10.1126/sciadv.abg1448DOI Listing
May 2021

Vintage analysis of Chinese Baijiu by GC and H NMR combined with multivariable analysis.

Food Chem 2021 Apr 23;360:129937. Epub 2021 Apr 23.

China National Research Institute of Food and Fermentation Industries Co. Ltd, Beijing 100015, China. Electronic address:

Economical-driven counterfeit and inferior aged Chinese Baijiu has caused serious concern of publicity in China. In this study, a total of 167 authentic Chinese Baijiu samples with different vintages including 3 flavor types were carefully collected. Gas chromatography (GC) was used to determine main volatile components and proton nuclear magnetic resonance (H NMR) spectroscopy was employed to obtain non-targeted fingerprints of Chinese Baijiu samples. Partial least squares regression (PLSR) models, which were confirmed by internal and external validation, were established for effectively identifying actual storage vintage of Chinese Baijiu with various brands, flavor types. Centering (Ctr), pareto scaling (Par), unit variance scaling (UV) data pretreatment methods, principal components (PCs), and three modified variable selection methods were proposed to successfully optimize the vintage model and effectively extract important vintage characteristic factors. This study demonstrated that NMR and GC combined with multivariate statistical analysis are effective tools for validating vintage authenticity of Chinese Baijiu.
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http://dx.doi.org/10.1016/j.foodchem.2021.129937DOI Listing
April 2021

Effects of crustacean hyperglycemic hormone (CHH) RNA interference on regulation of glucose metabolism in after ammonia-N exposure.

Br J Nutr 2021 May 14:1-40. Epub 2021 May 14.

The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.

To unveil the adaptation of Litopenaeus vannamei to elevated ambient ammonia-N, crustacean hyperglycemic hormone (CHH) was knocked down to investigate its function in glucose metabolism pathway under ammonia-N exposure. When CHH was silenced, haemolymph glucose increased significantly during 3-6 h, decreased significantly during 12-48 h, and recovered to the control groups' level at 72 h. After CHH knockdown, DA contents reduced significantly during 3-24 h, which recovered after 48 h. Besides, the expressions of GC and DA1R in the hepatopancreas decreased significantly, while DA4R increased significantly. Correspondingly, the contents of cAMP, cGMP and DAG and the expressions of PKA, PKG, AMPKα and AMPKγ were significantly downregulated, while the levels of PKC and AMPKβ were significantly upregulated. The expressions of CREB and GLUT2 decreased significantly, while GLUT1 increased significantly. Moreover, glycogen content, glycogen synthase and glycogen phosphorylase activities in hepatopancreas and muscle were significantly increased. Furthermore, the levels of key enzymes HK, PK and PFK in glycolysis, rate-limiting enzymes CS in TCA, and critical enzymes PEPCK, FBP and G6P in gluconeogenesis were significantly decreased in hepatopancreas. These results suggest that CHH affects DA, and then they affect their receptors respectively to transmit glucose metabolism signals into the hepatopancreas of L. vannamei under ammonia-N stress. CHH acts on cGMP-PKG-AMPKα-CREB pathway through GC, and CHH affects DA to influence cAMP-PKA-AMPKγ-CREB and DAG-PKC-AMPKβ-CREB pathways, thereby regulating GLUTs, inhibiting glycogen metabolism and promoting glycolysis and gluconeogenesis. This study contributes to further understand glucose metabolism mechanism of crustacean in response to environmental stress.
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http://dx.doi.org/10.1017/S0007114521001574DOI Listing
May 2021

Ambient ozone pollution at a coal chemical industry city in the border of Loess Plateau and Mu Us Desert: characteristics, sensitivity analysis and control strategies.

PeerJ 2021 27;9:e11322. Epub 2021 Apr 27.

College of Resource and Environment Engineering, Guizhou University, Guiyang, China.

In this study, ambient ozone (O) pollution characteristics and sensitivity analysis were carried out in Yulin, a city in the central area of the Loess Plateau during 2017 to 2019 summer. O concentrations increased for 2017 to 2019. Correlation and statistics analysis indicated high temperature (T > 25 °C, low relative humidity (RH < 60%), and low wind speed (WS < 3 m/s) were favorable for O formation and accumulation, and the O pollution days (MDA8 O > 160 µg/m) were predominantly observed when the wind was traveling from the easterly and southerly. O concentration in urban area of Yulin was higher than that in background. The pollution air masses from Fenwei Plain increase the level and duration of O pollution. In order to clarify the formation mechanism and source of O, online measurements of volatile organic compounds (VOCs) were conducted from 7 July to 10 August in 2019. The average of VOCs concentration was 26 ± 12 ppbv, and large amounts of alkenes followed by aromatics, characteristic pollutants of the coal chemical industry, were detected in the ambient air. To further measure the sensitivity, the observation-based model (OBM) simulation was conducted. Empirical Kinetic Modeling Approach (EKMA) plot and relative incremental reactivity (RIR) value indicated Yulin located on VOCs-limited regime. That implied a slight decrease of NO may increase O concentration. When the emission reduction ratio of anthropogenic VOCs/NO higher than 1:1, the O will decrease. O control strategies analysis shows that the O targets of 5% and 10% O concentration reductions are achievable through precursor control, but more effort is needed to reach the 30% and 40% reduction control targets.
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http://dx.doi.org/10.7717/peerj.11322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086580PMC
April 2021

Low-threshold and controllable nanolaser based on quasi-BIC supported by an all-dielectric eccentric nanoring structure.

Opt Express 2021 Apr;29(8):12634-12643

High-Q factor can enhance the interaction between light and matter, which is an important parameter to decrease the threshold of nanolasers. Here, we theoretically propose an eccentric nanoring structure with a high and controllable Q factor to realize a low-threshold and controllable nanolaser by amplifying the quasi-bound states in the continuum (quasi-BIC). The designed nanostructure supports a quasi-BIC because of the symmetry protection-breaking of the nanostructure. The quasi-BIC has a very high Q factor of about 9.6×10 and can also be adjusted by changing structural parameters. We use the energy level diagram of the four-level two-electron system to study the lasing action of the eccentric nanoring structure. The results show that the nanolaser has a relatively low threshold of about 6.46 μJ/cm. Furthermore, the lasing behavior can be tuned by controlling the structural parameters of the eccentric circular ring structure.
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http://dx.doi.org/10.1364/OE.420001DOI Listing
April 2021

Concentration and atmospheric transport of PM-bound polycyclic aromatic hydrocarbons at Mount Tai, China.

Sci Total Environ 2021 May 5;786:147513. Epub 2021 May 5.

Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Key Laboratory for Aerosol-Cloud-Precipitation of China Meteorological Administration, Nanjing University of Information Science & Technology, Nanjing 210044, China.

Atmospheric PM-bound polycyclic aromatic hydrocarbons (PAHs) pose a major threat to human health. At present, studies on PAHs in the atmosphere have mostly focused on their concentration levels and source apportionment, whereas studies on the vertical transport of PAHs in the atmosphere are limited. However, the vertical transport of PAHs is important for their diffusion near the ground and their long-range transport at higher altitude. In this study, PM samples were collected simultaneously at the summit and foot of Mount Tai (MT and MT, respectively) from May to June 2017, and the concentrations of 18 PAHs in the samples were determined. The total concentration of PAHs at MT was 2.406 ng m, which was well below the pollution levels of domestic cities, whereas that at MT was as high as 9.068 ng m, which was within the range of pollution levels in domestic cities. The total carcinogenic risk for both MT and MT was within the potential risk range. Given the source of PAHs and the diurnal variation of the planetary boundary layer, the PAHs showed opposite diurnal trends at MT and MT. Vertical transport was an important source of daytime PAHs at MT, and the vertical transport efficiency of PAHs decreased with an increasing ring number; this may be due to the combined effects of gas-particle partitioning and chemical reactions. Furthermore, PAHs originating in the surrounding high-emission provinces can affect the Mount Tai area via atmospheric trans-regional transport, and the BaP/BeP ratio is a useful indicator of the transport distance of PAHs.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147513DOI Listing
May 2021

Multiplex Electrochemiluminescence Polarization Assay Based on the Surface Plasmon Coupling Effect of Au NPs and Ag@Au NPs.

Anal Chem 2021 May 13. Epub 2021 May 13.

Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.

A novel multiplex electrochemiluminescence (ECL) polarization assay was developed to detect breast cancer-related genes BRCA1 and BRCA2 simultaneously based on the polarization characteristics of surface plasmon-coupled electrochemiluminescence (SPC-ECL). In this work, boron nitride quantum dots (BN QDs) were used as ECL emitters, and gold nanoparticles (Au NPs) and gold-coated silver nanoparticles (Ag@Au NPs) were employed as surface plasmon materials. The surface plasmon coupling resonance of different metal NPs not only enhanced the ECL intensity but also converted the isotropic emission into directional emission. This study revealed the relation between the structure of metal nanomaterials and SPC-ECL, and a high polarization-resolved sensing system was designed to detect multitarget DNA from 100 aM to 1 nM simultaneously. Polarization-based multiple ECL analysis has broad prospects in related cancer diagnosis and treatment evaluation.
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http://dx.doi.org/10.1021/acs.analchem.1c00757DOI Listing
May 2021

Causal structural covariance network revealing atrophy progression in Alzheimer's disease continuum.

Hum Brain Mapp 2021 May 12. Epub 2021 May 12.

Department of Radiology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

The structural covariance network (SCN) has provided a perspective on the large-scale brain organization impairment in the Alzheimer's Disease (AD) continuum. However, the successive structural impairment across brain regions, which may underlie the disrupted SCN in the AD continuum, is not well understood. In the current study, we enrolled 446 subjects with AD, mild cognitive impairment (MCI) or normal aging (NA) from the Alzheimer's Disease Neuroimaging Initiative (ADNI) database. The SCN as well as a casual SCN (CaSCN) based on Granger causality analysis were applied to the T1-weighted structural magnetic resonance images of the subjects. Compared with that of the NAs, the SCN was disrupted in the MCI and AD subjects, with the hippocampus and left middle temporal lobe being the most impaired nodes, which is in line with previous studies. In contrast, according to the 194 subjects with records on CSF amyloid and Tau, the CaSCN revealed that during AD progression, the CaSCN was enhanced. Specifically, the hippocampus, thalamus, and precuneus/posterior cingulate cortex (PCC) were identified as the core regions in which atrophy originated and could predict atrophy in other brain regions. Taken together, these findings provide a comprehensive view of brain atrophy in the AD continuum and the relationships among the brain atrophy in different regions, which may provide novel insight into the progression of AD.
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http://dx.doi.org/10.1002/hbm.25531DOI Listing
May 2021

CircRNA_2646 functions as a ceRNA to promote progression of esophageal squamous cell carcinoma via inhibiting miR-124/PLP2 signaling pathway.

Cell Death Discov 2021 May 11;7(1):99. Epub 2021 May 11.

Department of Thoracic Surgery, the First Affiliated Hospital, Sun Yat-sen University, 510080, Guangzhou, China.

MicroRNA-124 (miR-124) has been predicted as a tumor suppressor in esophageal squamous cell carcinoma (ESCC). However, factors contributing to miR-124 reduction remain unclear. Circular RNAs (circRNAs) are a new family of non-coding RNAs with gene regulatory potential via interacting with miRNAs. We predicted three circRNAs, including CircRNA_14359, CircRNA_2646, and CircRNA_129, that could interact with miR-124 by bioinformatics analysis and determined their expressions in ESCC tissues and adjacent normal tissues. We found that CircRNA_2646 was up-regulated in ESCC, negatively correlated with the expression of miR-124 and positively associated with TNM stage and lymph node metastasis of ESCC. Luciferase reporter assay showed that CircRNA_2646 interacted with miR-124 in ESCC Eca109 and TE-1 cells. Moreover, ectopical overexpression of CircRNA_2646 accelerated cell proliferation, migration, invasion, and epithelial-to-mesenchymal transition (EMT), but restoration of miR-124 abrogated these functions and promoted Bcl-2-dependent cell apoptosis. Furthermore, it was found that the oncogene Proteolipid Protein 2 (PLP2) was the target gene of miR-124. In Eca109 and TE-1 cells, restoration of miR-124 decreased the level of PLP2 and inhibited PLP2-induced cell proliferation, migration, invasion, and EMT, but enhanced cell apoptosis. The in vivo study confirmed that CircRNA_2646 promoted ESCC development by repressing miR-124 and activating PLP2. Taken together, we identified that CircRNA_2646 functioned as an inhibitor in miR-124 signaling pathway in ESCC for carcinogenesis and could be a promising target for ESCC therapy.
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http://dx.doi.org/10.1038/s41420-021-00461-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113544PMC
May 2021

Safety assessment of phytase transgenic maize 11TPY001 by 90-day feeding study in rats.

Food Chem Toxicol 2021 May 7:112254. Epub 2021 May 7.

NHC Key Laboratory of Food Safety Assessment, Chinese Academy of Medical Science Research Unit (2019RU014), China National Center for Food Safety Risk Assessment, Beijing 100021, China. Electronic address:

11TPY001 is a transgenic maize that expresses the Aspergillus niger phyA2 gene which could significantly improve phosphorus bioavailability in monogastric animals. The present study was conducted to investigate the potential health effects of phytase transgenic maize 11TPY001 through a 90-day subchronic rodent feeding study. Maize grains from 11TPY001 or its parental counterpart maize OSL963 were incorporated into rodent diets at 12.5%, 25% and 50% concentrations by mass and administered to Sprague-Dawley rats (n=10/sex/group) for 90 days. An additional control group of rats (n=10/sex/group) were fed with common maize Zhengdan958 diets at 50% by mass. All formulated diets were nutritionally balanced. Body weights, food intake, hematology, serum chemistry, absolute and relative organ weights were measured, and gross as well as microscopic pathology were examined. Compared with rats fed OSL963 maize and the common maize diet groups, no adverse diet-related differences were observed in rats fed 11TPY001 maize diets with respect to clinical signs of toxicity, body weight/gain, food consumption/efficiency, hematology, clinical chemistry, organ weights, and gross and microscopic pathology. Under the conditions of this study, the results indicated that 11TPY001 did not cause any treatment related adverse effects in rats compared with its non-transgenic parental maize OSL963.
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http://dx.doi.org/10.1016/j.fct.2021.112254DOI Listing
May 2021

Complete mitochondrial genomes of two insular races of swordtails from Taiwan, China (Lepidoptera: Papilionidae: ).

Mitochondrial DNA B Resour 2021 Apr 26;6(4):1557-1559. Epub 2021 Apr 26.

School of Agriculture, Yunnan University, Kunming, China.

The mitogenomes of two insular subspecies of , () and () from Taiwan Island, are reported. Both mitogenomes are circular, 15,228 bp and 15,240 bp in length respectively, and consist of 37 genes, including 13 PCGs, 22 tRNAs, and two rRNAs. The Bayesian phylogenetic tree containing the focal taxa and 31 other Papilioninae members clustered them with () (Alphéraky, 1897) and then () (Nicéville, 1900) inside tribe Leptocircini, which agrees with their taxonomic positions. The findings of this study would benefit future understanding of phylogeography and conservation of subgenus .
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http://dx.doi.org/10.1080/23802359.2021.1915719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079028PMC
April 2021

Laparoscopic vs. Open Repeat Hepatectomy for Recurrent Liver Tumors: A Propensity Score-Matched Study and Meta-Analysis.

Front Oncol 2021 22;11:646737. Epub 2021 Apr 22.

Department of Liver Surgery & Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China.

It remains unclear whether the short-term benefits of laparoscopic repeat hepatectomy (LRH) accrue to patients with recurrent liver tumors. The present study aimed to report our own center's experience and perform a meta-analysis to evaluate the safety and feasibility of LRH in comparison with open repeat hepatectomy (ORH) for treating recurrent liver tumors. A propensity score-matched study was performed including 426 patients receiving LRH or ORH for recurrent hepatocellular carcinoma between January 2017 and December 2018. Surgical outcomes and perioperative inflammation-based markers, including monocyte-to-lymphocyte ratio, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and systemic immune-inflammation index were collected from medical records and analyzed. Additionally, a systematic literature review was performed to identify relevant studies in PubMed, EMBASE, Web of Science, and Cochrane library databases up to October 1, 2020. Information including patient demographics, pathologic characteristics, and short-term outcomes was extracted and analyzed using random- or fixed-effects models. Of 68 LRHs, 57 were matched with an ORH finally. Our study demonstrated that LRH was significantly associated with less intraoperative blood loss (50 vs. 100 mL; < 0.001), lower rate of hepatic inflow occlusion (10.52 vs. 33.3%; = 0.003), and shorter postoperative hospital stay (5 vs. 6 days; = 0.001) after 1:1 propensity score matching. The operation time, rate of blood transfusion, and postoperative complications were similar between the two groups. Moreover, all four inflammation-based markers were significantly lower in LRH group on postoperative day 1. In the meta-analysis, a total of 12 studies comprising 1,315 patients receiving repeat hepatectomy met the selection criteria. Similar to our own study, the meta-analysis showed shorter hospital stay [standard mean difference (SMD) = -0.51, 95% confidence interval (CI) = -0.79 to -0.22, < 0.001], less intraoperative blood loss (SMD = -0.79, 95% CI = -1.11 to -0.47, < 0.001), and lower rate of major postoperative complications [odds ratio (OR) = 0.35, 95% CI = 0.19-0.66, = 0.001] in the LRH group. There was no difference in the field of overall postoperative complication and operation time between LRH and ORH groups. Compared with ORH, LRH results in relatively better surgical outcomes and faster postoperative recovery. It could be considered a feasible and effective option for the treatment of recurrent liver tumors.
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http://dx.doi.org/10.3389/fonc.2021.646737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100033PMC
April 2021

A characterization and prognosis prediction model for primary squamous cell carcinoma of the thyroid.

Gland Surg 2021 Apr;10(4):1325-1338

Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, China.

Background: Primary squamous cell carcinoma of the thyroid (PSCCTh) is a sporadic malignancy arising from the thyroid gland. The factors that affect treatment and survival in patients with PSCCTh remain unclear. Our study aims to characterize PSCCTh and establish a prognosis prediction model for patients with PSCCTh.

Methods: Clinical data and follow-up information for 277 patients from 1973 to 2016 were collected from the Surveillance, Epidemiology, and End Results Program (SEER) 18-registry database (RRID:SCR_003293). Univariate and multivariate Cox proportional hazards analyses and nomogram modeling of potential prognostic factors were conducted.

Results: Among the collected patient cases, 57% were female and 43% were male. The median survival of all cases was 6 months; by gender, median survival was 5 and 8 months in the female and male groups, respectively. Univariate and multivariate Cox proportional hazards analyses revealed that age, extent of disease (EOD), T stage, N stage, and treatment were independent prognostic indicators for overall survival (OS) and disease-specific survival (DSS) in patients with PSCCTh. In addition, it was confirmed that the established nomogram model had good consistency and discrimination for PSCCTh prognosis as measured by the concordance index (C-index), area under the receiver operating characteristic (ROC) curve (AUC), and calibration curves.

Conclusions: Our study indicates that age, EOD, T stage, N stage, and treatment may correlate with OS and DSS in patients with PSCCTh. Importantly, our nomogram prediction model, constructed using parameters including age, T stage, N stage, and treatment, may assist physicians in evaluating patients' prognoses and providing precise therapy for PSCCTh.
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http://dx.doi.org/10.21037/gs-20-847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102235PMC
April 2021

Upregulation of glucosamine-phosphate N-acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma.

Oncol Lett 2021 Jun 22;21(6):488. Epub 2021 Apr 22.

Department of Thoracic and Cardiovascular Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, P.R. China.

Lung adenocarcinoma, a type of non-small cell lung cancer, is the leading cause of cancer death worldwide. Great efforts have been made to identify the underlying mechanism of adenocarcinoma, especially in relation to oncogenes. The present study by integrating computational analysis with western blotting, aimed to understand the role of the upregulation of glucosamine-phosphate N-acetyltransferase 1 (GNPNAT1) in carcinogenesis. In the present study, publicly available gene expression profiles and clinical data were downloaded from The Cancer Genome Atlas to determine the role of GNPNAT1 in lung adenocarcinoma (LUAD). In addition, the association between LUAD susceptibility and GNPNAT1 upregulation were analyzed using Wilcoxon signed-rank test and logistic regression analysis. In LUAD, GNPNAT1 upregulation was significantly associated with disease stage [odds ratio (OR)=2.92, stage III vs. stage I], vital status (dead vs. alive, OR=1.89), cancer status (tumor status vs. tumor-free status, OR=1.85) and N classification (yes vs. no, OR=1.75). Cox regression analysis and the Kaplan-Meier method were utilized to evaluate the association between GNPNAT1 expression and overall survival (OS) time in patients with LUAD. The results demonstrated that patients with increased GNPNAT1 expression levels exhibited a reduced survival rate compared with those with decreased expression levels (P=8.9×10). In addition, Cox regression analysis revealed that GNPNAT1 upregulation was significantly associated with poor OS time [hazard ratio (HR): 1.07; 95% confidence interval (CI): 1.04-1.10; P<0.001]. The gene set enrichment analysis revealed that 'cell cycle', 'oocyte meiosis', 'pyrimidine mediated metabolism', 'ubiquitin mediated proteolysis', 'one carbon pool by folate', 'mismatch repair progesterone-mediated oocyte maturation' and 'basal transcription factors purine metabolism' were differentially enriched in the GNPNAT1 high-expression samples compared with GNPNAT1 low-expression samples. The aforementioned pathways are involved in the pathogenesis of LUAD. The findings of the present study suggested that GNPNAT1 upregulation may be considered as a promising diagnostic and prognostic biomarker in patients with LUAD. In addition, the aforementioned pathways may be pivotal pathways perturbed by the abnormal expression of GNPNAT1 in LUAD. The findings of the present study demonstrated the therapeutic value of the regulation of GNPNAT1 in lung adenocarcinoma.
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http://dx.doi.org/10.3892/ol.2021.12750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100941PMC
June 2021

A Double-Antibody Sandwich ELISA for Sensitive and Specific Detection of Swine Fibrinogen-Like Protein 1.

Front Immunol 2021 23;12:670626. Epub 2021 Apr 23.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, China.

Fibrinogen-like protein 1 (FGL1), a member of the fibrinogen family, is a specific hepatocyte mitogen. Recently, it has been reported that FGL1 is the main inhibitory ligand of lymphocyte activating gene 3 (LAG3). Furthermore, the FGL1-LAG3 pathway has a synergistic effect with programmed death 1 (PD-1)/programmed death ligand 1 (PD-L1) pathway and is regarded as a promising immunotherapeutic target. However, swine FGL1 (sFGL1) has not been characterized and its detection method is lacking. In the study, the sFGL1 gene was amplified from the liver tissue of swine and then inserted into a prokaryotic expression vector, pQE-30. The recombinant plasmid pQE30-sFGL1 was transformed into JM109 competent cells. The recombinant sFGL1 was induced expression by isopropyl-β-d-thiogalactoside (IPTG) and the purified sFGL1 was used as an antigen to produce mouse monoclonal antibody (mAb) and rabbit polyclonal antibody (pAb). After identification, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for sensitive and specific detection of sFGL1 was developed. Swine FGL1 in samples was captured by anti-sFGL1 mAb followed by detection with anti-sFGL1 rabbit pAb and HRP-conjugated goat anti-rabbit IgG. The limit of detection of the developed sFLG1-DAS-ELISA is 35 pg/ml with recombinant sFLG1. Besides, it does not show cross-reactivity with the control protein. Then serum samples of PRRSV-negative and -positive pigs were tested with the established DAS-ELISA and calculated according to the equation of y=0.0735x+0.0737. The results showed that PRRSV infection enhanced the serum FGL1 levels significantly. Our research provides a platform for the research on the functional roles of swine FGL1.
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http://dx.doi.org/10.3389/fimmu.2021.670626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102871PMC
April 2021

Heavy ozone pollution episodes in urban Beijing during the early summertime from 2014 to 2017: Implications for control strategy.

Environ Pollut 2021 Apr 27;285:117162. Epub 2021 Apr 27.

Environment Research Institute, Shandong University, Qingdao, 266237, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.

Ground-level ozone (O) has become the principal air pollutant in Beijing during recent summers. In this context, an investigation of ambient concentrations and variation characteristics of O and its precursors in May and June from 2014 to 2017 in a typical urban area of Beijing was carried out, and the formation sensitivity and different causes of heavy O pollution (HOP, daily maximum 8-h O (MDA8h O)>124 ppbv) were analyzed. The results showed that the monthly assessment values of the O concentrations (the 90 percentile MDA8h O within one month) were highest in May or June from 2014 to 2017, and the values presented an overall increasing trend. During this period, the number of O pollution days (MDA8h O > 75 ppbv) also showed an increasing trend. During the HOP episodes, the concentrations of volatile organic compounds (VOCs), nitrogen oxides (NO), and carbon monoxide (CO) were higher than their respective mean values in May and June, and the meteorological conditions were more conducive to atmospheric photochemical reactions. The HOP episodes were mainly caused by local photochemical formation. From 2014 to 2017, O formation during the HOP episodes shifted from VOC and NO mixed-limited to VOC-limited conditions, and O formation was most sensitive to anthropogenic VOCs. Six categories of VOC sources were identified, among which vehicular exhaust contributed the most to anthropogenic VOCs. The VOC concentrations and OFPs of anthropogenic sources have decreased significantly in recent years, indicating that VOC control measures have been effective in Beijing. Nevertheless, NO concentrations did not show an evident decreasing trend in the same period. Therefore, more attention should be devoted to O pollution control in May and June; control measure adjustments are needed according to the changes in O precursors, and coordinated control of VOCs and NO should be strengthened in long-term planning.
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http://dx.doi.org/10.1016/j.envpol.2021.117162DOI Listing
April 2021

Ipsilateral hypoperfusion caused by intracerebral steal phenomenon after carotid artery stenting: a case report.

BMC Neurol 2021 May 8;21(1):188. Epub 2021 May 8.

Department of Neurosurgery, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, No. 305 Zhongshan East Road, Jiangsu Province, 210002, Nanjing, People's Republic of China.

Background: Abnormal hypoperfusion on the surgical side after carotid artery stenting is rare. Neurological deterioration caused by it is deceptive, which can easily lead to misdiagnosis. The mechanism of hypoperfusion has rarely been demonstrated. We present here a fully studied case with a high probability of intracerebral steal phenomenon.

Case Presentation: A 68-year-old male with severe right internal carotid artery stenosis and left internal carotid artery occlusion underwent right stenosis stent implantation. Restlessness and left limb hemiplegia occurred within 24 h after the procedure, which was similar to hyperperfusion syndrome. However, postoperative computerized tomography perfusion (CTP) revealed abnormal hypoperfusion in the right hemisphere. Transcranial Doppler (TCD) also showed decreased flow velocity in the right middle cerebral artery, and increased flow velocity in the right anterior cerebral artery. We considered that intracerebral steal phenomenon might be the cause, then hypervolemic therapy was accepted and the symptoms completely resolved after 3 days.

Conclusions: Ipsilateral hypoperfusion is rarely seen after carotid artery stenting. Intracerebral steal phenomenon may be the underlying mechanism. CTP or TCD is helpful for the early detection of this adverse event.
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http://dx.doi.org/10.1186/s12883-021-02208-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106848PMC
May 2021

Weakly Supervised Low-Rank Representation for Hyperspectral Anomaly Detection.

IEEE Trans Cybern 2021 May 7;PP. Epub 2021 May 7.

In this article, we propose a weakly supervised low-rank representation (WSLRR) method for hyperspectral anomaly detection (HAD), which formulates deep learning-based HAD into a low-lank optimization problem not only characterizing the complex and diverse background in real HSIs but also obtaining relatively strong supervision information. Different from the existing unsupervised and supervised methods, we first model the background in a weakly supervised manner, which achieves better performance without prior information and is not restrained by richly correct annotation. Considering reconstruction biases introduced by the weakly supervised estimation, LRR is an effective method for further exploring the intricate background structures. Instead of directly applying the conventional LRR approaches, a dictionary-based LRR, including both observed training data and hidden learned data drawn by the background estimation model, is proposed. Finally, the derived low-rank part and sparse part and the result of the initial detection work together to achieve anomaly detection. Comparative analyses validate that the proposed WSLRR method presents superior detection performance compared with the state-of-the-art methods.
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http://dx.doi.org/10.1109/TCYB.2021.3065070DOI Listing
May 2021

Enhancing fluorescence of benzimidazole derivative via solvent-regulated ESIPT and TICT process: A TDDFT study.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 21;258:119862. Epub 2021 Apr 21.

Institute of Atomic and Molecular Physics, Jilin University, Changchun 130012, China. Electronic address:

In this work, we use density functional theory and time dependent density functional theory to explore the ESIPT and TICT process of 6-(1H-Benzoimidazol-2-yl)-2,3-dimethoxy-phenol (BIDOP) in cyclohexane (CHX) and tetrahydrofuran (THF) solvent, respectively. It reveals that ESIPT process of BIDOP can occur in both CHX and THF solvent at the first excited state with similar reaction barrier. Remarkably, compared to barrierless from keto (K*) to TICT state of BIDOP in THF solvent, the reaction barrier between K* and TICT state is up to 20.28 kcal/mol for in CHX that TICT process is inhibited in CHX solvent. The absence of nonradiative decay TICT state of BIDOP in CHX solvent induces higher fluorescence in CHX compared to in THF solvent. These findings indicate that CHX solvent can effectively enhance fluorescence of BIDOP. Our study highlights a convenient approach for enhancing fluorescence and is significant for photophysics and photobiology field.
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http://dx.doi.org/10.1016/j.saa.2021.119862DOI Listing
April 2021

Evolving cryo-EM structural approaches for GPCR drug discovery.

Structure 2021 May 2. Epub 2021 May 2.

Drug Discovery Biology Theme, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville 3052, VIC, Australia; ARC Centre for Cryo-Electron Microscopy of Membrane Proteins, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville 3052, VIC, Australia. Electronic address:

G protein-coupled receptors (GPCRs) are the largest class of cell surface drug targets. Advances in stabilization of GPCR:transducer complexes, together with improvements in cryoelectron microscopy (cryo-EM) have recently been applied to structure-assisted drug design for GPCR agonists. Nonetheless, limitations in the commercial application of these approaches, including the use of nanobody 35 (Nb35) to aid complex stabilization and the high cost of 300 kV imaging, have restricted broad application of cryo-EM in drug discovery. Here, using the PF 06882961-bound GLP-1R as exemplar, we validated the formation of stable complexes with a modified Gs protein in the absence of Nb35. In parallel, we compare 200 versus 300 kV image acquisition using a Falcon 4 or K3 direct electron detector. Moreover, the 200 kV Glacios-Falcon 4 yielded a 3.2 Å map with clear density for bound drug and multiple structurally ordered waters. Our work paves the way for broader commercial application of cryo-EM for GPCR drug discovery.
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http://dx.doi.org/10.1016/j.str.2021.04.008DOI Listing
May 2021

Realization of Ethylene Production from Its Quaternary Mixture through Metal-Organic Framework Materials.

ACS Appl Mater Interfaces 2021 May 6. Epub 2021 May 6.

Department of Chemistry, University of Texas at San Antonio, One UTSA Circle, San Antonio, Texas 78249-0698, United States.

Ethylene production from oxidative coupling of methane is a sustainable and economically attractive alternative to that through traditional hydrocarbon cracking technology. However, efficient ethylene separation from the complex reaction mixture is a daunting challenge that hinders the practical adoption of this technology. Herein, we report the efficient adsorptive separation of the CH/CO/CH/CH mixture using three representative metal-organic frameworks (MOFs) (UTSA-74, MOF-74, and HKUST-1) with diverse open metal sites. The efficient separation relies on tuning the selectivity through the convergence of characteristics including Lewis acidity of open metal sites, pore space, and cooperative binding behavior. The separation performance of these materials has been evaluated through single-component gas adsorption and dynamic breakthrough experiments. HKUST-1 provides the highest separation potential (4.1 mmol/g) thanks to its simultaneously high ideal adsorbed solution theory (IAST) selectivity and ethylene adsorption capacity, representing a benchmark material for such a challenging quaternary separation.
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http://dx.doi.org/10.1021/acsami.1c03923DOI Listing
May 2021

Epidemiology of Drug- and Herb-Induced Liver Injury Assessed for Causality Using the Updated RUCAM in Two Hospitals from China.

Biomed Res Int 2021 24;2021:8894498. Epub 2021 Feb 24.

Department of Pharmacy, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

Drug- and herb-induced liver injury (DILI and HILI) is an increasingly common and serious condition. Here, data for DILI and HILI patients from two large tertiary hospitals were retrospectively analyzed. Patient characteristics, causes and severity of DILI and HILI, the correlation between expression of p62 and the severity of DILI and HILI, treatment of DILI and HILI, and the prognostic factors of DILI and HILI were studied. A total of 82 patients with DILI and HILI were recruited for the study. Most patients presented with hepatocellular injury, followed by cholestatic injury and mixed injury. Our results indicate that traditional Chinese medicine or herbal and dietary supplements were the prevalent causal agents of HILI, which was characterized by higher frequencies of hepatocellular injury. Expression of p62 in the liver correlated with the severity of DILI and HILI. Improvements in the results of the liver enzymatic tests correlated with alanine transaminase (ALT) levels upon the first diagnosis of DILI and HILI and with the hepatocellular type of DILI and HILI. In conclusion, we provide an epidemiological assessment of DILI and HILI based on causality using the updated RUCAM on patients from two hospitals in China. ALT levels at first diagnosis and the hepatocellular type of injury may be prognostic factors of DILI and HILI.
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http://dx.doi.org/10.1155/2021/8894498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067772PMC
February 2021

Faded Critical Dynamics in Adult Moyamoya Disease Revealed by EEG and fMRI.

Oxid Med Cell Longev 2021 16;2021:6640108. Epub 2021 Apr 16.

Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China.

Criticality is considered a dynamic signature of healthy brain activity that can be measured on the short-term timescale with neural avalanches and long-term timescale with long-range temporal correlation (LRTC). It is unclear how the brain dynamics change in adult moyamoya disease (MMD). We used BOLD-fMRI for LRTC analysis from 16 hemorrhagic () and 34 ischemic () patients and 25 healthy controls. Afterwards, they were examined by EEG recordings in the eyes-closed (EC), eyes-open (EO), and working memory (WM) states. The EEG data of 11 and 13 patients and 21 healthy controls were in good quality for analysis. Regarding the 4 metrics of neural avalanches (e.g., size (), duration (), value, and branching parameter ()), both MMD subtypes exhibited subcritical states in the EC state. When switching to the WM state, remained inactive, while surpassed controls and became supercritical ( < 0.05). Regarding LRTC, the amplitude envelope in the EC state was more analogous to random noise in the MMD patients than in controls. During state transitions, LRTC decreased sharply in the controls but remained chaotic in the MMD individuals ( < 0.05). The spatial LRTC reduction distribution based on both EEG and fMRI in the EC state implied that, compared with controls, the two MMD subtypes might exhibit mutually independent but partially overlapping patterns. The regions showing decreased LRTC in both EEG and fMRI were the left supplemental motor area of and right pre-/postcentral gyrus and right inferior temporal gyrus of . This study not only sheds light on the decayed critical dynamics of MMD in both the resting and task states for the first time but also proposes several EEG and fMRI features to identify its two subtypes.
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http://dx.doi.org/10.1155/2021/6640108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064775PMC
April 2021

Increasing prevalence of NAFLD/NASH among children, adolescents and young adults from 1990 to 2017: a population-based observational study.

BMJ Open 2021 May 4;11(5):e042843. Epub 2021 May 4.

Department of Epidemiology, School of Public Health, Fudan University, Shanghai, China

Objective: To describe the prevalence and variations of non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH) among children and adolescents (CADs) and young adults (YADs).

Design: A population-based observational study.

Setting: Annual cases and prevalence of NAFLD/NASH from 1990 to 2017, by sex, region and country were collected from the Global Burden of Disease database.

Main Outcome Measures: The estimated annual percentage change, which was calculated by a regression line, was used to quantify the temporal trends in NAFLD/NASH burden among young people at the global, regional and national levels.

Results: Globally, NAFLD/NASH incidence increased from 19.34 million in 1990 to 29.49 million in 2017 among CADs, with an annual increase of 1.35%. Additionally, in YADs, the number of cases and NAFLD/NASH prevalence significantly increased during this period, independent of sex and region. The greatest NAFLD/NASH increase was in North Africa and the Middle East. Almost all countries showed an increasing trend from 1990 to 2017, with the most pronounced increase observed in the developed regions.

Conclusions: The epidemiology of NAFLD/NASH in young people has changed considerably over the last three decades. Both the prevalence and number of cases have increased irrespective of sex, age and region. This phenomenon can result in a predictable increase in chronic liver disease burden in the near future. Understanding the prevalence of NAFLD/NASH and its variations is of paramount importance to develop strategies to implement public health policy.
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http://dx.doi.org/10.1136/bmjopen-2020-042843DOI Listing
May 2021

Multiomics Analysis Reveals New Insights into the Apple Fruit Quality Decline under High Nitrogen Conditions.

J Agric Food Chem 2021 May 4. Epub 2021 May 4.

State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, Shandong, China.

Excessive application of nitrogen (N) fertilizer is common in Chinese apple production. High N reduced the contents of soluble sugar and total flavonoids by 16.05 and 19.01%, respectively, resulting in poor fruit quality. Moreover, high N increased the total N and decreased the total C and C/N ratio of apple fruits. On the basis of the transcriptomic, proteomic, and metabolomic analyses, the global network was revealed. High N inhibited the accumulation of carbohydrates (sucrose, glucose, and trehalose) and flavonoids (rhamnetin-3--rutinoside, rutin, and trihydroxyisoflavone-7--galactoside) in fruits, and more C skeletons were used to synthesize amino acids and their derivatives (especially low C/N ratio, e.g., arginine) to be transferred to N metabolism. This study revealed new insights into the decline in soluble sugar and flavonoids caused by high N, and hub genes (MD07G1172700, MD05G1222800, MD16G1227200, MD01G1174400, and MD02G1207200) and hub proteins (PFK, gapN, and HK) were obtained.
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http://dx.doi.org/10.1021/acs.jafc.1c01548DOI Listing
May 2021

On the efficient modeling of generic source directivity in Gaussian beam tracing.

J Acoust Soc Am 2021 Apr;149(4):2743

Department of Mechanical and Aerospace Engineering, Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China.

For large-distance sound propagation with complex obstacles, the Gaussian beam tracing (GBT) method is often applied. An omnidirectional source model is commonly implemented in GBT, which, however, neglects the influence of generic directivity patterns of practical acoustic problems. There have been efforts to synthesize or reproduce the target directivity pattern over an observation surface by using multiple distributed point sources. However, the efficiency and applicability of general applications still call for improvement. More specifically, rays from each of the point sources and their traces in the space should be computed and superposed to estimate the sound field, making the computational cost largely dependent on the complexity of the source directivity pattern. In this work, a complex-valued radiation function model is developed to realize the generic source directivity for GBT computation. One advantage of the method is that only one source is required such that computation cost can be greatly reduced. Rays are emitted from the source with direction-dependent amplitude and phase to realize the target directivity pattern. The development of the radiation function is associated with the GBT method. The verification cases show that this method can give good agreement with analytical or wave-based numerical solutions. Capabilities of modeling a complex source model of the spinning sound field to mimic the propeller noise are studied, and the result matches well with analytical solutions. Finally, a demonstration case of a four-propeller-powered drone in an urban region is conducted.
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http://dx.doi.org/10.1121/10.0004318DOI Listing
April 2021

RIP1 Perturbation Induces Chondrocyte Necroptosis and Promotes Osteoarthritis Pathogenesis Targeting BMP7.

Front Cell Dev Biol 2021 16;9:638382. Epub 2021 Apr 16.

Beijing Key Laboratory of Sports Injuries, Department of Sports Medicine, Institute of Sports Medicine of Peking University, Peking University Third Hospital, Beijing, China.

Osteoarthritis (OA) is a highly prevalent and debilitating joint disorder that characterized by progressive destruction of articular cartilage. There is no effective disease-modifying therapy for the condition due to limited understanding of the molecular mechanisms on cartilage maintenance and destruction. Receptor-interacting protein kinase 1 (RIP1)-mediated necroptosis plays a vital role in various diseases, but the involvement of RIP1 in OA pathogenesis remains largely unknown. Here we show that typical necrotic cell morphology is observed within human OA cartilage samples , and that RIP1 is significantly upregulated in cartilage from both OA patients and experimental OA rat models. Intra-articular RIP1 overexpression is sufficient to induce structural and functional defects of cartilage in rats, highlighting the crucial role of RIP1 during OA onset and progression by mediating chondrocyte necroptosis and disrupting extracellular matrix (ECM) metabolism homeostasis. Inhibition of RIP1 activity by its inhibitor necrostatin-1 protects the rats from trauma-induced cartilage degradation as well as limb pain. More importantly, we identify bone morphogenetic protein 7 (BMP7) as a novel downstream target that mediates RIP1-induced chondrocyte necroptosis and OA manifestations, thereby representing a non-canonical regulation mode of necroptosis. Our study supports a model whereby the activation of RIP1-BMP7 functional axis promotes chondrocyte necroptosis and subsequent OA pathogenesis, thus providing a new therapeutic target for OA.
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http://dx.doi.org/10.3389/fcell.2021.638382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085605PMC
April 2021

The Roles of Reduced Folate Carrier-1 (RFC1) A80G (rs1051266) Polymorphism in Congenital Heart Disease: A Meta-Analysis.

Med Sci Monit 2021 May 3;27:e929911. Epub 2021 May 3.

Department of Cardiovascular Surgery, Gansu Provincial Hospital, Lanzhou, Gansu, China (mainland).

BACKGROUND We performed the present study to better elucidate the correlation of reduced folate carrier-1 (RFC1) A80G (rs1051266) polymorphism with the risk of congenital heart disease (CHD). MATERIAL AND METHODS According to the designed search strategy, a systematic literature search was performed through the PubMed, Cochrane Library, Web of Science, EMBASE, CNKI, VIP, and Wan Fang databases to collect published case-control studies on the correlation between RFC1 A80G polymorphism and CHD. All relevant studies up to October 1, 2019 were identified. The odds ratio (OR) and 95% confidence interval (CI) of the genotype distribution were used as the effect indicators. RESULTS A total of 6 eligible studies was finally included in our meta-analysis, including 724 children with CHD, 760 healthy children, 258 mothers of the children with CHD, and 334 mothers of healthy control children. The meta-analysis revealed that for fetal analysis, only in the heterozygous model (GA vs GG, OR=1.36, 95% CI [1.06, 1.75], P=0.02) was RFC1 A80G polymorphism associated with risk of CHD. In maternal analysis, 3 genetic models of RFC1 A80G polymorphism increased the risk of CHD: the allelic model (A vs G, OR=1.36, 95% CI [1.07, 1.71], P=0.01), the homozygote model (AA vs GG, OR=2.99, 95%CI [1.06, 8.41], P=0.04), and the dominance model (GA+AA vs GG, OR=1.53, 95%CI [1.08, 2.16], P=0.02). CONCLUSIONS The maternal RFC1 A80G polymorphism has a strong correlation with CHD. Compared with the G allele, the A allele increases the risk of CHD by 0.36-fold.
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http://dx.doi.org/10.12659/MSM.929911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103792PMC
May 2021