Publications by authors named "Xin Ye"

676 Publications

Identifying transcriptional programs underlying cancer drug response with TraCe-seq.

Nat Biotechnol 2021 Sep 16. Epub 2021 Sep 16.

Department of Discovery Oncology, Genentech Inc., South San Francisco, CA, USA.

Genetic and non-genetic heterogeneity within cancer cell populations represent major challenges to anticancer therapies. We currently lack robust methods to determine how preexisting and adaptive features affect cellular responses to therapies. Here, by conducting clonal fitness mapping and transcriptional characterization using expressed barcodes and single-cell RNA sequencing (scRNA-seq), we have developed tracking differential clonal response by scRNA-seq (TraCe-seq). TraCe-seq is a method that captures at clonal resolution the origin, fate and differential early adaptive transcriptional programs of cells in a complex population in response to distinct treatments. We used TraCe-seq to benchmark how next-generation dual epidermal growth factor receptor (EGFR) inhibitor-degraders compare to standard EGFR kinase inhibitors in EGFR-mutant lung cancer cells. We identified a loss of antigrowth activity associated with targeted degradation of EGFR protein and an essential role of the endoplasmic reticulum (ER) protein processing pathway in anti-EGFR therapeutic efficacy. Our results suggest that targeted degradation is not always superior to enzymatic inhibition and establish TraCe-seq as an approach to study how preexisting transcriptional programs affect treatment responses.
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http://dx.doi.org/10.1038/s41587-021-01005-3DOI Listing
September 2021

Spatiotemporal evolution law and driving force of mining city patterns.

Environ Sci Pollut Res Int 2021 Sep 13. Epub 2021 Sep 13.

College of Mining Engineering, Heilongjiang University of Science and Technology, Harbin, 150022, People's Republic of China.

Urban transformation is an inevitable trend for mining cities to achieve sustainable development. Analyzing the spatiotemporal evolution laws and driving mechanisms of mining cities is necessary for providing a scientific basis for their transformation. In this study, Jixi was taken as an example, which is a typical mining city in China. Based on geographic information system, various mathematical statistical analysis methods were used to quantitatively analyze the evolution pattern of mining cities. In addition, the driving mechanisms of land expansion in mining cities were examined further. The results showed that (1) the urban land in the mining city is mainly distributed in low-lying areas, and land expansion mainly occurred in flat areas. (2) Based on the distribution of mineral resources, the land use pattern of mining cities was scattered; with steady economic development, the urban spatial pattern tends to be compact. (3) The spatial pattern of mining cities is affected by natural, economic, and policy factors. The results reveal the spatiotemporal evolution law and driving mechanism of the patterns in mining cities, thereby providing a scientific basis for the sustainable development planning and land management of mining cities.
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http://dx.doi.org/10.1007/s11356-021-16488-5DOI Listing
September 2021

[Effects of slope position and aspect on structure and species diversity of shrub community in the Jiajin Mountains, Sichuan Province, China].

Ying Yong Sheng Tai Xue Bao 2020 Aug;31(8):2507-2514

College of Forestry, Sichuan Agricultural University, Chengdu 611130, China.

We examined the impacts of slope direction and position on the community structure and species diversity of shrub community in Jiajin Mountains. The results showed that a total of 186 vascular plant species belonging to 50 families and 127 genera were recorded in the 24 sample plots. 32 species belonging to 14 families, 22 genera were recorded in the shrubs layer, with most species distributed at lower position of the shady slope. 154 species belonging to 43 families and 109 genera were recorded in the herbaceous layer, with the species number being the lowest on the shady slope. The average height of the shrub layer showed a decreasing order of sunny slope, semi-sunny slope, and shady slope, while an opposite order was observed for the average density. The average height of the herb layer was in the decreasing order of the shady slope, semi-sunny slope, sunny slope, whereas no significant changes were observed for the average density. The slope position had significant effect only on the average height of the herbaceous layer on the sunny slope. The overall level of species diversity in the shrub layer on the shady slope was relatively high, while that in the herbaceous layer was relatively high at the lower position of the sunny slope and at the middle position of the shady slope. Slope aspect had significant effect on species richness index, Shannon diversity index, Simpson dominance index, Pielou evenness index of shrub and herb layers except for Simpson index of herb layer. Slope position did not affect these indices. The interaction effect of slope aspect and position on the diversity index for herb layer was greater than that for the shrub layer. Results from redundancy analysis showed that species diversity was related to sin(aspect), cos(aspect), and community structure.
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http://dx.doi.org/10.13287/j.1001-9332.202008.004DOI Listing
August 2020

Effect of 580 °C (20 h) Heat Treatment on Mechanical Properties of 25Cr2NiMo1V Rotor-Welded Joints of Oscillating Arc (MAG) Narrow Gap Thick Steel.

Materials (Basel) 2021 Aug 11;14(16). Epub 2021 Aug 11.

Shanghai Turbine Factory, Shanghai Electric Power Station Equipment Co., Ltd., Shanghai 201100, China.

The thick plate narrow gap welding of 25Cr2NiMo1V rotor steel is achieved by metal active gas arc welding, in which the weld gap was 18.04-19.9 mm. After welding, the weldment was heat treated at 580 °C (20 h). The impact and tensile properties in the as-welded and heat-treated were studied. The results show that after heat treatment, the coarse carbides in the center of the weld were transformed into fine granular carbides distributed along the grain boundaries, and the quantity of carbide precipitates in the weld near the fusion line was reduced. The tensile fracture mode changed from a ductile fracture to a combination of brittle and ductile fractures, and the tensile strength of the weld metal changed from 605 MPa to 543 MPa. After heat-treated, the radiation zone of the weld center changed from a brittle fracture to a combination of brittle and ductile fractures, and the impact energy changed from 141 J to 183 J; the characteristics of the brittle fracture in the radial zone of the fusion line were more obvious, and the impact energy changed from 113 J to 95 J. Therefore, after heat treatment, the toughness of the welded metal was improved, without reducing the strength and hardness of the welded metal to a large extent.
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http://dx.doi.org/10.3390/ma14164498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400107PMC
August 2021

Effect of statins use on risk and prognosis of breast cancer: a meta-analysis.

Anticancer Drugs 2021 Aug 16. Epub 2021 Aug 16.

Nanjing University of Chinese Medicine, Nanjing Department of General Surgery Department of Oncology, Lianshui County People's Hospital, Huaian, Jiangsu, China.

The findings regarding the association between statins use and breast cancer are inconsistent. Given the widely and long-term use of statins as first choice drug for dyslipidemia, we conducted this meta-analysis for better understanding the associations between statins use and the risk and prognosis of breast cancer. Articles regarding effect of statins use on risk, prognosis of breast cancer and published before January 2021 were searched in the following databases: Web of Science, PubMed, EMBASE, Medline and Google Scholar. Odds ratios (ORs)/relative risks (RRs) or hazard ratios (HRs) and their 95% confidence intervals (CIs) were computed to generate a pooled effect size and 95% CI. The meta-analysis showed no significant association between statins use and risk of breast cancer (OR/RR = 1.02; 95% CI, 0.97-1.08; I2 = 76.1%; P < 0.001). The meta-analysis showed that statins use was associated with lower breast cancer recurrence, all-cause mortality and disease-specific mortality (breast cancer recurrence: HR = 0.75; 95% CI, 0.67-0.84; I2 = 31.7%; P = 0.154; all-cause mortality: HR = 0.82; 95% CI, 0.77-0.89; I2 = 67.5%; P < 0.001; and disease-specific mortality: HR = 0.82; 95% CI, 0.72-0.93; I2 = 83.6%; P < 0.001). Overall, in this report we demonstrated that the use of statins can improve the prognosis of breast cancer patients including lower risks of breast cancer recurrence, all-cause and cancer-specific mortality, though statins therapy may not have an impact on reducing the risk of breast cancer.
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http://dx.doi.org/10.1097/CAD.0000000000001151DOI Listing
August 2021

Contralateral protective effect against repeated bout of damaging exercise: A meta-analysis.

Res Sports Med 2021 Jul 26:1-20. Epub 2021 Jul 26.

Department of Rehabilitation Sciences, University of Hartford, West Hartford, CT, USA.

The purpose was to summarize the studies examining the contralateral protective effect on the maximal strength in the subsequent bout of muscle-damaging exercise. The literature search was conducted through CINAHL plus, SportDiscus, and PubMed. Hedge's g effect size (ES) and 95% confidence intervals (CIs) were computed using a random effects model. From 14 papers and 25 ESs, the mean ES for contralateral repeated bout effect (CL-RBE) on 1-, 2-, and 3-day post maximal strength were -0.61 (95% CI = -0.80, -0.41), -0.50 (95% CI = -0.67, -0.33), and -0.74 (95% CI = -1.01, -0.48), respectively. For moderator analyses, the mean ESs were not influenced by type (isometric vs. isokinetic) of strength, but CL-RBE on maximal strength was influenced by duration (≤6 weeks) between bouts. Therefore, the meta-analysis demonstrated that an initial bout of exercise induces the protective effect on contralateral limb muscles regardless of the different type of strength, but can be affected by different duration (≤6 weeks) between exercise bouts.
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http://dx.doi.org/10.1080/15438627.2021.1954512DOI Listing
July 2021

A high-specificity flap probe-based isothermal nucleic acid amplification method based on recombinant FEN1-Bst DNA polymerase.

Biosens Bioelectron 2021 Nov 15;192:113503. Epub 2021 Jul 15.

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China. Electronic address:

The COVID-19 pandemic has unfortunately demonstrated how easily infectious diseases can spread and harm human life and society. As of writing, pandemic has now been on-going for more than one year. There is an urgent need for new nucleic acid-based methods that can be used to diagnose pathogens early, quickly, and accurately to effectively impede the spread of infections and gain control of epidemics. We developed a flap probe-based isothermal nucleic acid amplification method that is triggered by recombinant FEN1-Bst DNA polymerase, which-through enzymatic engineering-has both DNA synthesis, strand displacement and cleavage functions. This novel method offers a simpler and more specific probe-primer pair than those of other isothermal amplifications. We tested the method's ability to detect SARS-CoV-2 (both ORF1ab and N genes), rotavirus, and Chlamydia trachomatis. The limits of detection were 10 copies/μL for rotavirus, C. trachomatis, and SARS-CoV-2 N gene, and 100 copies/μL for SARS-CoV-2 ORF1ab gene. There were no cross-reactions among 11 other common pathogens with characteristics similar to those of the test target, and the method showed 100% sensitivity and 100% specificity in clinical comparisons with RT-PCR testing. In addition to real-time detection, the endpoint could be displayed under a transilluminator, which is a convenient reporting method for point-of-care test settings. Therefore, this novel nucleic acid senor has great potential for use in clinical diagnostics, epidemic prevention, and epidemic control.
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http://dx.doi.org/10.1016/j.bios.2021.113503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280370PMC
November 2021

Polysaccharides Ameliorate Diet-Induced Gallstone Formation by Modulating Synthesis of Bile Acids and the Gut Microbiota.

Front Pharmacol 2021 1;12:701003. Epub 2021 Jul 1.

Digestive Endoscopic Center, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Cholesterol gallstone (CG) disease has relationships with several metabolic abnormalities. polysaccharides (APS) have been shown to have multiple benefits against metabolic disorders. We attempted to uncover the effect and mechanism of action of APS on diet-induced CG formation in mice. Animals were fed a chow diet or lithogenic diet (LD) with or without APS supplementation. The effect of APS on CG formation was evaluated. The level of individual bile acids (BAs) in gallbladder bile and ileum were measured by liquid chromatography-tandem mass spectrometry. Real-time reverse transcription-quantitative polymerase chain reaction and western blotting were used to assess expression of the genes involved in BA metabolism and the enterohepatic circulation. Cecal contents were collected to characterize microbiota profiles. APS ameliorated LD-induced CG formation in mice. APS reduced the level of total cholesterol, bile acid hydrophobicity index and cholesterol saturation index in gallbladder bile. The protective effect of APS might result from reduced absorption of cholic acid in the intestine and increased hepatic BA synthesis. APS relieved the LD-induced activation of the intestinal farnesoid X receptor and decreased ileal expression of fibroblast growth factor 15. In the liver, expression of cytochrome P450 () enzyme and was increased, whereas expression of adenosine triphosphate-binding cassette () transporters and was decreased by APS. APS improved the diversity of the gut microbiota and increased the relative abundance of the Bacteroidetes phylum. APS had demonstratable benefits against CG disease, which might be associated with enhanced BA synthesis and improved gut microbiota. Our results suggest that APS may be a potential strategy for the prevention of CG disease.
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http://dx.doi.org/10.3389/fphar.2021.701003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281024PMC
July 2021

Sodium butyrate alleviates cholesterol gallstones by regulating bile acid metabolism.

Eur J Pharmacol 2021 Oct 14;908:174341. Epub 2021 Jul 14.

Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Medicine, Shanghai, China; Digestive Endoscopic Center, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, China. Electronic address:

Cholesterol overloading and bile acid metabolic disorders play an important role in the onset of cholesterol gallstone (CGS). Short-chain fatty acids (SCFAs) can regulate bile acid metabolism by modulating the gut microbiota. However, the role and mechanism by which sodium butyrate (NaB) targets bile acids to attenuate CGS are still unknown. In this study, continuous administration of 12 mg/day for 8 weeks was decreased the incidence of gallstones induced by lithogenic diet (LD) from 100% to 25%. NaB modulated SCFAs and improved the gut microbiota. The remodeling of the gut microbiota changed the bile acid compositions and decreased cecal tauro-α-muricholic acid (T-α-MCA) and tauro-β-muricholic acid (T-β-MCA) which are effective farnesoid X receptor (FXR) antagonists. The quantitative real-time PCR examination showed that NaB significantly increased levels of ileal Fxr, fibroblast growth factor-15 (Fgf-15) and small heterodimer partner (Shp) mRNA and subsequently inhibited bile acid synthesis. In addition, NaB enhanced bile acid excretion by increasing the levels of hepatic multidrug resistance protein 2 (Mdr2) and bile salt export pump (Bsep) mRNA, and it enhanced bile acid reabsorption in the intestine by increasing the levels of ileal bile acid transporter (Ibat) mRNA. In addition, NaB reduced the absorption of cholesterol in the intestine and inhibited the excretion of cholesterol in the liver, which reduced the cholesterol concentration in serum and bile. Furthermore, the protective effects of NaB administration were abolished by FXR antagonists. Taken together, our results suggest that NaB mitigates CGS by modulating the gut microbiota to regulate the FXR-FGF-15/SHP signaling pathway.
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http://dx.doi.org/10.1016/j.ejphar.2021.174341DOI Listing
October 2021

Computed tomography-guided percutaneous microwave ablation for pulmonary multiple ground-glass opacities.

J Cancer Res Ther 2021 Jul;17(3):811-813

Department of Oncology, Shandong Key Laboratory of Rheumatic Disease and Translational Medicine, Shandong Lung Cancer Institute, Shandong Provincial Qianfoshan Hospital, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong, China.

With the wide application of low-dose computed tomography (CT) and high-resolution CT, the increasing cases of pulmonary nodules are identified through routine thoracic imaging examination, many of which are presented as multiple ground-glass opacities (GGOs). The multiple GGOs could be divided into four pathological types and usually got different combined mutation patterns, suggesting that each GGO is an independent event and should be treated separately. However, there is no established guideline to the treatment of multiple GGOs so far. Here, we report a multiple GGOs case with a different mutation pattern treated by CT-guided percutaneous microwave ablation.
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http://dx.doi.org/10.4103/jcrt.jcrt_531_21DOI Listing
July 2021

Short-term efficacy and safety of callispheres drug-loaded microsphere embolization in primary hepatocellular carcinoma.

J Cancer Res Ther 2021 Jul;17(3):733-739

Department of Oncology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital; Shandong Key Laboratory of Rheumatic Disease and Translational Medicine, Shandong Lung Cancer Institute, Jinan, China.

Background: Drug-eluting beads transarterial chemoembolization (DEB-TACE) is a newly developed local regional therapy for improving the efficacy and safety of conventional transarterial chemoembolization (cTACE), which is now universally used to treat patients with unresectable liver cancer.Cohort studies, clinical trials, and meta-analysis have shown DEB-TACE to be associated with favorable treatment responses, prolonged survival, and at least similar safety profile when compared with cTACE.

Aims And Objectives: This study was to evaluate the short term clinical efficacy, side effects, and risk factors affecting the clinical effectiveness of CalliSpheres drug loaded bead transcatheter arterial chemoembolization (DEB TACE) in the treatment of primary hepatocellular carcinoma (HCC).

Materials And Methods: A total of 172 consecutive patients with HCC undergoing DEB TACE (loaded with doxorubicin) from January 2017 to December 2018 were prospectively enrolled. Short term local tumor response was evaluated by the modified RECIST criteria. Postoperative complications and liver function disorders were analyzed based on examinations and clinical symptoms.

Results: The median follow up period was 310 days. Based on the modified response evaluation criteria in solid tumors criteria, objective response rates(complete response [CR] + partial response [PR]) were 78.7%, 71.6%, and 63.2%, and disease control rates(CR + PR + stable disease) were 95.3%, 92.1%, and 85.9% at 2, 4, and 6 months posttreatment, respectively. Multivariate logistic regression analysis showed that nodule number >3, high BCLC stage, no vascular leak, and previous conventional TACE treatment were associated with poor ORR (P < 0.05). Postoperation, liver function showed transient changes. Postoperative complications were tolerated and relieved by symptomatic treatment. The average interval of TACE before D TACE was 43 days, compared with 70 days for average interval of DEB TACE. The average hospital stay was 1.87 days.

Conclusion: DEB TACE has improved short term efficacy and lower incidence of complications in primary HCC and prolongs the interval of TACE. It significantly increases the ORR, especially in patients with no extra hepatic metastasis pretreatment. DEB usage actually improves treatment efficacy and provides more benefits to patients. KEY WORDS: Drug-loaded bead-transcatheter arterial chemoembolization, hepatocellular carcinoma, microsphere embolization.
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http://dx.doi.org/10.4103/jcrt.JCRT_1848_20DOI Listing
July 2021

Low urinary iodine is a protective factor of central lymph node metastasis in papillary thyroid cancer: a cross-sectional study.

World J Surg Oncol 2021 Jul 12;19(1):208. Epub 2021 Jul 12.

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuaifuyuan Wangfujing Dongcheng District, Beijing, 100730, China.

Background: An abrupt increase of thyroid cancer has been witnessed paralleling the supplemented iodine intake in formerly iodine-deficient countries. And increased iodine intake has been linked to the rising incidence rate of papillary thyroid cancer (PTC). However, the correlation between iodine and clinicopathological features of PTC has not been well-characterized. This study aimed to investigate the associations between iodine intake and the clinicopathological features of PTC patients.

Methods: Three hundred and fifty-nine PTC patients who received surgical treatment in Peking Union Medical College Hospital from May 2015 to November 2020 were retrospectively reviewed. The associations between urinary iodine (UI), urinary iodine/creatinine ratio (UI/U-Cr), and the clinicopathological features of PTC were analyzed. Univariate and multivariate analysis were performed to investigate the relationship between UI level and central lymph node metastasis (CLNM).

Results: There were no significant differences in UI in different groups according to the variables studied, except that patients with CLNM had higher UI level than CLNM(-) patients. No associations were found between UI/U-Cr and clinicopathological features except variant subtypes (classic/follicular). After dividing patients into high-iodine group and low-iodine group, more patients were found to have CLNM in the high-iodine group (p = 0.02). In addition, younger age, larger tumor size, and classic variant were positively correlated with CLNM (p < 0.05). Univariate analysis showed that insufficient iodine intake (≤ 99 μg/L) was associated with decreased CLNM risk in PTC. And after defining insufficient iodine intake as ≤ 109 μg/L and above requirements as ≥ 190 μg/L, multivariate analysis showed that lower iodine was associated with CLNM in total population of PTC (OR 0.53, 95% CI 0.31-0.91) and in PTC < 1 cm (papillary thyroid microcarcinoma, PTMC) (OR 0.43, 95% CI 0.21-0.87).

Conclusions: Low iodine was a protective factor for CLNM in papillary thyroid cancer, particularly in those < 1 cm. These results indicated that iodine may not only be an initiator of tumorigenesis, but also a promoter of the development of PTC.
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http://dx.doi.org/10.1186/s12957-021-02302-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276512PMC
July 2021

Endoscopic radiofrequency ablation may improve overall survival in patients with inoperable ampullary carcinoma.

Dig Endosc 2021 Jul 7. Epub 2021 Jul 7.

Departments of, Endoscopy, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.

Objectives: Patients with advanced ampullary carcinoma (AC) who are unsuitable for surgery are most likely to have poor outcomes. The role of endoscopic radiofrequency ablation (RFA) in this population has not been fully defined. We aimed to assess the short- and long-term outcomes of RFA in a large cohort of AC patients.

Methods: In this retrospective study, data of consecutive patients with pathologically proven AC who underwent successful endobiliary RFA and/or stent placement were collected. All patients did not undergo surgical resection. The primary outcome was overall survival (OS). The secondary outcomes included clinical success and adverse events.

Results: A total of 85 patients, 50 in the RFA plus stenting group and 35 in the stenting alone group, were identified. The median OS was significantly longer in the RFA group than in the stenting alone group (16.9 vs. 9.8 months, P < 0.001). In multivariable Cox analysis, RFA (hazards ratio 0.408; 95% confidence interval 0.235-0.706; P = 0.001) was the only independent OS predictor. Eight patients with stage II tumors, exclusively from the RFA group, survived for more than 3 years. Clinical success was comparable between the two groups (96% vs. 100%, P = 0.231). Early adverse events between the two groups were similar (10% vs. 2.9%, P = 0.206); however, late biliary/pancreatic stenoses occurred in three RFA patients who were successfully managed with endoscopic interventions.

Conclusions: Endoscopic RFA appears to prolong patients' survival with acceptable safety; it may therefore be a feasible treatment option for patients with inoperable ampullary cancers.
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http://dx.doi.org/10.1111/den.14078DOI Listing
July 2021

Could concurrent biopsy and microwave ablation be reliable? Concordance between frozen section examination and final pathology in CT-guided biopsy of lung cancer.

Int J Hyperthermia 2021 ;38(1):1031-1036

Department of Oncology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Key Laboratory of Rheumatic Disease and Translational Medicine, Shandong Lung Cancer Institute, Jinan, Shandong, China.

Purpose: Microwave ablation combined with concurrent biopsy has been used for lung cancer. Frozen section (FS) diagnosis is an important supplement for the final pathology (FP). Thus, a retrospective study was conducted to evaluate the concordance between FS examination and FP in the computed tomography (CT)-guided biopsy of lung cancer.

Materials And Methods: Patients who underwent percutaneous transthoracic needle lung biopsies and were diagnosed using both intraoperative FS examination and FP were retrospectively enrolled. Concordance between FS findings and FP in the diagnosis of malignant lung cancer and the definitive histology types were recorded.

Results: Overall, 163 patients were enrolled. The concordance rate in the diagnosis of malignant tumors was 96.3%. The definitive histology types were concordant between FS examinations and FP in 112 patients (68.7%). Lung cancers undefined with FS but diagnosed as adenocarcinoma with FP were the most common type, observed in 18 patients. The concordance in the histology type was lower for those requiring immunohistochemistry for FP diagnoses (47.3 79.6%,  < 0.000). Concordance rates differed for the different histology types diagnosed using FP (adenocarcinoma squamous cell carcinoma small-cell lung cancer others, 76.6 56.2 69.2 0.0%,  < 0.000).

Conclusions: FS was inferior to FP in the diagnosis of definitive histology types, but had a high concordance with FP in the diagnosis of malignant lung cancer.
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http://dx.doi.org/10.1080/02656736.2021.1947528DOI Listing
August 2021

A Novel Lightweight Wearable Soft Exosuit for Reducing the Metabolic Rate and Muscle Fatigue.

Biosensors (Basel) 2021 Jun 30;11(7). Epub 2021 Jun 30.

Shenzhen Institute of Adanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Wearable robotic devices have been proved to considerably reduce the energy expenditure of human walking. It is not only suitable for healthy people, but also for some patients who require rehabilitation exercises. However, in many cases, the weight of soft exosuits are relatively large, which makes it difficult for the assistant effect of the system to offset the metabolic consumption caused by the extra weight, and the heavy weight will make people uncomfortable. Therefore, reducing the weight of the whole system as much as possible and keeping the soft exosuit output power unchanged, may improve the comfort of users and further reduce the metabolic consumption. In this paper, we show that a novel lightweight soft exosuit which is currently the lightest among all known powered exoskeletons, which assists hip flexion. Indicated from the result of experiment, the novel lightweight soft exosuit reduces the metabolic consumption rate of wearers when walking on the treadmill at 5 km per hour by 11.52% compared with locomotion without the exosuit. Additionally, it can reduce more metabolic consumption than the hip extension assisted (HEA) and hip flexion assisted (HFA) soft exosuit which our team designed previously, which has a large weight. The muscle fatigue experiments show that using the lightweight soft exosuit can also reduce muscle fatigue by about 10.7%, 40.5% and 5.9% for rectus femoris, vastus lateralis and gastrocnemius respectively compared with locomotion without the exosuit. It is demonstrated that decreasing the weight of soft exosuit while maintaining the output almost unchanged can further reduce metabolic consumption and muscle fatigue, and appropriately improve the users' comfort.
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http://dx.doi.org/10.3390/bios11070215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301778PMC
June 2021

T-cell infiltration, contribution and regulation in the central nervous system post-traumatic injury.

Cell Prolif 2021 Aug 29;54(8):e13092. Epub 2021 Jun 29.

Department of Basic Medicine Sciences, and Department of Orthopaedics of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

T cells participate in the repair process and immune response in the CNS post-traumatic injury and play both a beneficial and harmful role. Together with nerve cells and other immune cells, they form a microenvironment in the CNS post-traumatic injury. The repair of traumatic CNS injury is a long-term process. T cells contribute to the repair of the injury site to influence the recovery. Recently, with the advance of new techniques, such as mass spectrometry-based flow cytometry, modern live-cell imaging, etc, research focusing on T cells is becoming one of the valuable directions for the future therapy of traumatic CNS injury. In this review, we summarized the infiltration, contribution and regulation of T cells in post-traumatic injury, discussed the clinical significance and predicted the future research direction.
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http://dx.doi.org/10.1111/cpr.13092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349661PMC
August 2021

Development and validation of tumor-to-blood based nomograms for preoperative prediction of lymph node metastasis in lung cancer.

Thorac Cancer 2021 08 24;12(15):2189-2197. Epub 2021 Jun 24.

Department of Thoracic Surgery, Beijing Chao-Yang Hospital, Beijing, China.

Background: To develop and validate tumor-to-blood based nomograms for preoperative prediction of lymph node (LN) metastasis in patients with lung cancer (LC).

Methods: A prediction model was developed in a primary cohort comprising 330 LN stations from patients with pathologically confirmed LC, these data having been gathered from January 2016 to June 2019. Tumor-to-blood variables of LNs were calculated from positron emission tomography-computed tomography (PET-CT) images of LC and the short axis diameters of LNs were measured on CT images. Tumor-to-blood variables, number of stations suspected of harboring LN metastasis according to PET, and independent clinicopathological risk factors were included in the final nomograms. After being internally validated, the nomograms were used to assess an independent validation cohort containing 101 consecutive LN stations accumulated from July 2019 to March 2020.

Results: Four tumor-to-blood variables (left atrium, inferior vena cava, liver, and aortic arch) and the maximum standardized uptake value (SUVmax) for LNs were found to be significantly associated with LN status (p < 0.001 for both primary and validation cohorts). Five predictive nomograms were built. Of these, one with LN SUVmax/left atrium SUVmax was found to be optimal for predicting LN status with AUC 0.830 (95% confidence interval [CI]: 0.774-0.886) in the primary cohort and AUC 0.865 (95% CI: 0.782-0.948) in the validation cohort. All models showed good discrimination, with a modest C-index, and good calibration in both primary and validation cohorts.

Conclusions: We have developed tumor-to-blood based nomograms that incorporate identified clinicopathological risk factors and facilitate preoperative prediction of LN metastasis in LC patients.
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http://dx.doi.org/10.1111/1759-7714.14066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327690PMC
August 2021

New Type of Thermoelectric CdSSe Nanowire Chip.

ACS Appl Mater Interfaces 2021 Jul 24;13(26):30959-30966. Epub 2021 Jun 24.

Beijing Key Lab of Nano-photonics and Ultrafine Optoelectronic Systems School of Physics Beijing Institute of Technology, Beijing 100081, P. R. China.

Facing the increasingly serious problem of environmental pollution and energy waste, the thermoelectric generator has been attracting more and more attention owing to its advantages including low cost, no pollution, and good stability. The family of thermoelectric material is constantly extended with enhanced performance. Note that nanostructuring can enhance thermoelectric performance. However, the most recent excellent material with effective thermoelectric transformation reported from bulk materials has definite benefits to the practical application compared to nanomaterials. In this work, a nanostructure integrated macroscale thermoelectric chip, that is an alloyed band gap gradient macroscale chip (1.0 cm × 2.0 cm) composed of CdSSe nanowires, has been proven as an excellent thermoelectric generator for the first time. A high Seebeck coefficient of -152.4 μV/K and the average output voltage of 10.8 mV are obtained after optimizing the electrode patterns and distance between electrodes. More interestingly, upon illumination by white light from a xenon lamp, a photo-thermoelectric output voltage is greatly elevated to 45 mV due to the high concentration of photogenerated carriers. The CdSSe thermoelectric chip also shows good repeatability and high stability with a relative error of <6%. No study on the thermoelectric performance of such an alloyed band gap gradient macroscale chip is mentioned before. The results illustrate a bright avenue to realize a type of light-modulated macroscale thermoelectric chips by nanostructure, allowing such kinds of CdSSe chips to be used to generate electric energy in the near future.
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http://dx.doi.org/10.1021/acsami.1c04206DOI Listing
July 2021

The study of spectroscopy and vibrational assignments of high nitrogen material 1,1'-azobis-1,2,3-triazole.

J Mol Model 2021 Jun 23;27(7):205. Epub 2021 Jun 23.

School of Physical Science and Technology, Southwest Jiaotong University, Key Laboratory of Advanced Technologies of Materials, Ministry of Education of China, Chengdu, 610031, People's Republic of China.

Benefiting from the new strategy of oxidative azo coupling of the N-NH moiety, a series of energetic nitrogen-rich molecules with long catenated nitrogen chains have been successfully synthesized. As one of them, the synthesized 1,1'-azobis-1,2,3-triazole shows excellent thermal stability, great explosive performance, and special photochromic properties, which has caused widespread concern. To further characterize its performance, the structural, electronic, vibrational, mechanical, and thermodynamic properties of 1,1'-azobis-1,2,3-triazole were investigated based on the first-principles density functional theory calculations. The obtained structural parameters are consistent with previous results. We used the band structure, density of states, Mulliken charges, bond populations, and electron density to analyze the electronic properties and chemical bonding. The vibrational frequency regions (396.51-3210.12 cm) were assigned to the corresponding vibrational modes. Furthermore, mechanical properties of 1,1'-azobis-1,2,3-triazole are also calculated. Finally, the thermodynamic properties of 1,1'-azobis-1,2,3-triazole were calculated, including the specific heat at constant volume C, temperature*entropy TS, enthalpy H, Gibbs free energy G, and Debye temperature Θ.
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http://dx.doi.org/10.1007/s00894-021-04822-0DOI Listing
June 2021

Multicentre study of microwave ablation for pulmonary oligorecurrence after radical resection of non-small-cell lung cancer.

Br J Cancer 2021 Aug 15;125(5):672-678. Epub 2021 Jun 15.

Department of Oncology, Shandong Lung Cancer Institute, Shandong Provincial Qianfoshan Hospital, The First Affiliated Hospital of Shandong First Medical University, Jinan, China.

Background: Microwave ablation (MWA) is an effective minimally invasive technique for lung tumours. We aim to evaluate its role for pulmonary oligorecurrence after radical surgery of non-small-cell lung cancer (NSCLC).

Methods: From June 2012 to Jan 2020, a total of 103 patients with pulmonary oligorecurrence after previous radical surgical resection of NSCLC were retrospectively analysed. The primary endpoint was postoperative progression-free survival (PFS). Secondary endpoints were postoperative overall survival (OS), patterns of failure, complications and predictive factors associated with prognosis.

Results: Of the 103 patients identified, 135 pulmonary oligorecurrences developed at a median interval of 34.8 months. In total, 143 sessions of MWA were performed to ablate all the nodules. The median PFS and OS were 15.1 months and 40.6 months, respectively. After MWA, 15 (14.6%) patients had local recurrence as the first event, while intrathoracic oligorecurrence and distant metastases were observed in 45 (43.7%) and 20 (19.4%) patients, respectively. In the multivariate analysis, local recurrence and intrathoracic oligorecurrence were not significant predictors for OS (P = 0.23 and 0.26, respectively). However, distant metastasis was predictive of OS (HR = 5.37, 95% CI, 1.04-27.84, P = 0.04).

Conclusion: MWA should be considered to be an effective and safe treatment option for selected patients with pulmonary oligorecurrence after NSCLC radical surgical resection.
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http://dx.doi.org/10.1038/s41416-021-01404-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405665PMC
August 2021

Direct and indirect associations between childhood socioeconomic status and cognitive function in the middle-aged and older adults in China.

Aging Ment Health 2021 Jun 14:1-8. Epub 2021 Jun 14.

China Center for Health Development Studies, Peking University, Beijing, China.

Objectives: To investigate whether childhood socioeconomic status (SES) is associated with cognitive function, and what factors might mediate the associations.

Method: Using data from the China Health and Retirement Longitudinal Study (CHARLS) and within frameworks of the latency model, the pathway model and the accumulation model, we quantified direct and indirect pathways between childhood SES and cognitive function for Chinese middle-aged and older adults aged 45+ by structural equations modeling.

Results: We found significant direct, indirect and total effects of childhood SES on cognitive function at baseline. The indirect effects were mediated through educational attainment, household consumption, smoking behaviors and social engagement. At follow-ups, cognitive enhancement can be made by indirect pathways through educational attainment, improvement of household consumption and social engagement.

Conclusion: Our results supported the latency model, the pathway model and the accumulation model when considering pathways linking childhood SES to cognitive function. The findings underscored the value of taking early interventions to improve SES and cognitive function, especially among those with low childhood SES.
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http://dx.doi.org/10.1080/13607863.2021.1935459DOI Listing
June 2021

Ligand conjugate SAR and enhanced delivery in NHP.

Mol Ther 2021 Jun 4. Epub 2021 Jun 4.

Genevant Sciences Corporation, Vancouver, BC V5T 4T5, Canada. Electronic address:

N-Acetylgalactosamine (GalNAc) conjugated short interfering RNAs (siRNAs) are a leading RNA interference (RNAi) platform allowing targeted inhibition of disease-causing genes in hepatocytes. More than a decade of development has recently resulted in the first approvals for this class of drugs. While substantial effort has been made to improve nucleic acid modification patterns for better payload stability and efficacy, relatively little attention has been given to the GalNAc targeting ligand. In addition, the lack of an intrinsic endosomal release mechanism has limited potency. Here, we report a stepwise analysis of the structure activity relationships (SAR) of the components comprising these targeting ligands. We show that there is relatively little difference in biological performance between bi-, tri-, and tetravalent ligand structures while identifying other features that affect their biological activity more significantly. Further, we demonstrate that subcutaneous co-administration of a GalNAc-functionalized, pH responsive endosomal release agent markedly improved the activity and duration of effect for siRNA conjugates, without compromising tolerability, in non-human primates. These findings could address a significant bottleneck for future siRNA ligand conjugate development.
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http://dx.doi.org/10.1016/j.ymthe.2021.06.002DOI Listing
June 2021

Long non-coding RNA CASC9/microRNA-590-3p axis participates in lutein-mediated suppression of breast cancer cell proliferation.

Oncol Lett 2021 Jul 20;22(1):544. Epub 2021 May 20.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.

Previous studies have shown that lutein can inhibit the proliferation of breast cancer cells. However, the mechanism of lutein inhibiting the proliferation of breast cancer cells remains unclear. The present study aimed to determine whether the long non-coding RNA (lncRNA) Cancer Susceptibility 9 (CASC9)/microRNA (miR)-590-3p axis participates in the antiproliferative effects of lutein via lncRNA microarray hybridization, reverse transcription-quantitative PCR, dual-luciferase reporter and MTT assays. The results demonstrated that CASC9 was the most significantly downregulated lncRNA in MCF7 cells treated with lutein. miR-590-3p was identified as the target of CASC9. In addition, lutein downregulated CASC9 expression and upregulated miR-590-3p expression in dose- and time-dependent manners, respectively. CASC9 knockdown or overexpression of miR-590-3p inhibited the proliferation of breast cancer cells. Notably, simultaneous transfection with miR-590-3p mimics and CASC9 small interfering RNA increased the potency of lutein in inhibiting the proliferation of breast cancer cells. Taken together, these results suggest that the CASC9/miR-590-3p axis participates in the antiproliferative effects of lutein on breast cancer.
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http://dx.doi.org/10.3892/ol.2021.12805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161424PMC
July 2021

Affinity Mass Spectrometry-Based Fragment Screening Identified a New Negative Allosteric Modulator of the Adenosine A Receptor Targeting the Sodium Ion Pocket.

ACS Chem Biol 2021 06 28;16(6):991-1002. Epub 2021 May 28.

iHuman Institute, ShanghaiTech University, Shanghai 201210, China.

Allosteric ligands provide new opportunities to modulate G protein-coupled receptor (GPCR) function and present therapeutic benefits over orthosteric molecules. Negative allosteric modulators (NAMs) can inhibit the activation of a receptor and downstream signal transduction. Screening NAMs for a GPCR target is particularly challenging because of the difficulty in distinguishing NAMs from antagonists bound to the orthosteric site as they both show inhibitory effects in receptor signaling assays. Here we report an affinity mass spectrometry (MS)-based approach tailored to screening potential NAMs of a GPCR target especially from fragment libraries. Compared to regular surface plasmon resonance or NMR-based methods for fragment screening, our approach features a reduction of the protein and compound consumption by 2-4 orders of magnitude and an increase in the data acquisition speed by 2-3 orders of magnitude. Our affinity MS-based fragment screening led to the identification of a new NAM of the adenosine A receptor (AAR) bearing an unprecedented azetidine moiety predicted to occupy the allosteric sodium binding site. Molecular dynamics simulations, ligand structure-activity relationship (SAR) studies, and in-solution NMR analyses further revealed the unique binding mode and antagonistic property of this compound that differs considerably from HMA (5-(,-hexamethylene)amiloride), a well-characterized NAM of AAR. Taken together, our work would facilitate fragment-based screening of allosteric modulators, as well as guide the design of novel NAMs acting at the sodium ion pocket of class A GPCRs.
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http://dx.doi.org/10.1021/acschembio.0c00899DOI Listing
June 2021

Differences in morphological and physiological features of citrus seedlings are related to Mg transport from the parent to branch organs.

BMC Plant Biol 2021 May 27;21(1):239. Epub 2021 May 27.

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Background: In this study, we aimed to test the hypothesis that magnesium (Mg) remobilization in citrus plants is regulated by Mg supply and contributes to differences in the growth of the parent and branch organs. Citrus seedlings were grown in sand under Mg deficient (0 mmol Mg L, -Mg) and Mg sufficient (2 mmol Mg L, + Mg) conditions. The effects on biomass, Mg uptake and transport, gas exchange and chlorophyll fluorescence, as well as related morphological and physiological parameters were evaluated in different organs.

Results: Mg deficiency significantly decreased plant biomass, with a decrease in total plant biomass of 39.6%, and a greater than twofold decrease in the branch organs compared with that of the parent organs. Reduced photosynthesis capacity was caused by a decreased in pigment levels and photosynthetic electron transport chain disruption, thus affecting non-structural carbohydrate accumulation and plant growth. However, the adaptive responses of branch leaves to Mg deficiency were greater than those in parent leaves. Mg deficiency inhibited plant Mg uptake but enhanced Mg remobilization from parent to branch organs, thus changing related growth variables and physiological parameters, including protein synthesis and antioxidant enzyme activity. Moreover, in the principal components analysis, these variations were highly clustered in both the upper and lower parent leaves, but highly separated in branch leaves under the different Mg conditions.

Conclusions: Mg deficiency inhibits the growth of the parent and branch organs of citrus plants, with high Mg mobility contributing to differences in physiological metabolism. These findings suggest that Mg management should be optimized for sustainable citrus production.
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http://dx.doi.org/10.1186/s12870-021-03028-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157678PMC
May 2021

Successful prenatal therapy of anti-CD36-mediated severe FNAIT by deglycosylated antibodies in a novel murine model.

Blood 2021 05 26. Epub 2021 May 26.

Institute of Blood Transfusion, Guangzhou Blood Center, China, Guangzhou, China.

Recent studies have demonstrated that maternal anti-CD36 antibodies represent a frequent cause of fetal/neonatal alloimmune thrombocytopenia (FNAIT) in Asian and African populations. However, little is known about the pathomechanism and antenatal treatment of anti-CD36-mediated FNAIT. Here, we established a novel animal model to examine the clinical features of pups from immunized Cd36-/- female mice after breeding with wild-type male mice. Mild thrombocytopenia was observed, but high pup mortality was also documented (40.26%). IVIG (1 g/kg) administration on days 7, 12, and 17 to immunized Cd36-/- mothers after breeding reduced fetal death (12.70%). However, delaying the IVIG administration series on days 10, 15, and 20 did not reduce fetal death (40.00%). In contrast, injection of deglycosylated anti-CD36 (deg-anti-CD36) polyclonal antibodies (5 mg/kg) on days 10, 15, and 20 significantly reduced fetal death (5.26%). Subsequently, monoclonal antibodies (mAbs) against mouse CD36 were developed, and one clone producing high-affinity anti-CD36 (termed 32-106) effectively inhibited maternal antibody binding and was therefore selected. Using the same approach of deg-anti-CD36, the administration of deg-32-106 significantly reduced fetal death (2.17%). Furthermore, immunized Cd36-/- mothers showed placenta deficiency. Accordingly, maternal anti-CD36 antibodies inhibited angiogenesis of placenta endothelial cells, which could be restored by deg-32-106. In summary, maternal anti-CD36 antibodies caused a high frequency of fetal death in our animal model, associated with placental dysfunction. This deleterious effect could be diminished by the antenatal administration of IVIG and deg-mAb 32-106. Interestingly, treatment with deg-32-106 appears more beneficial considering the lower dose, later start of treatment, and therapy success.
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http://dx.doi.org/10.1182/blood.2021011131DOI Listing
May 2021

Role of each step in the combined treatment of reactive ion etching and dynamic chemical etching for improving the laser-induced damage resistance of fused silica.

Opt Express 2021 Apr;29(8):12365-12380

We investigate the role of each step in the combined treatment of reactive ion etching (RIE) and dynamic chemical etching (DCE) for improving the laser-induced damage resistance of fused silica optics. We employ various surface analytical methods to identify the possible damage precursors on fused silica surfaces treated with different processes (RIE, DCE, and their combination). The results show that RIE-induced defects, including F contamination, broken Si-O bonds, luminescence defects (i.e., NBOHCs and ODCs), and material densification, are potential factors that limit the improvement of laser-induced damage resistance of the optics. Although being capable of eliminating the above factors, the DCE treatment can achieve rough optical surface with masses of exposed scratches and pits which might serve as reservoirs of the deposits such as inorganic salts, thus limiting the further improvement in damage resistance of fused silica. The study guides us to a deep understanding of the laser-induced damage process in achieving fused silica optics with enhanced resistance to laser-induced damage by the combined treatment of RIE and DCE.
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http://dx.doi.org/10.1364/OE.415438DOI Listing
April 2021

[Study on oral absorption mechanisms of puerarin in nanocrystals self-stabilized pickering emulsion].

Zhongguo Zhong Yao Za Zhi 2021 Apr;46(8):2051-2060

College of Pharmaceutical Sciences, Southwest University Chongqing 400716, China.

Nanocrystals self-stabilized Pickering emulsion(NSSPE) is a new kind of emulsion where only nanocrystals of poorly soluble drugs are used as stabilizers. Our previous study showed that NSSPE with Ligusticum chuanxiong oil as the main oil phase can significantly promote oral absorption of puerarin. The present study aimed to explore its absorption mechanism in oral administration. The in vitro dissolution test was carried out to study the effect of NSSPE on release of puerarin. The effects and mechanism of NSSPE on uptake and transport of puerarin across Caco-2 cell were investigated. The results showed that the drug release rate of NSSPE was similar to that of nanocrystals, with their cumulative dissolution of puerarin not affected by pH of releasing mediums, both significantly higher than that of crude material. The uptake of puerarin in NSSPE was concentration-dependent and significantly higher than that of solution or surfactant stabilized emulsion. Genistein and indomethacin, inhibitors of lipid rafts/caveolin, could significantly reduce the uptake of puerarin in NSSPE. Compared with solution, NSSPE and surfactants stabilized emulsion obviously increased transport rate K_a and apparent permeability coefficient P_(app) of puerarin in AP → BL direction, but there was no significant difference in BL → AP direction. It could be inferred that there were both passive and active transport mechanisms, as well as lipid raft/caveolin mediated endocytosis for absorption of NSSPE. The promoted oral absorption of puerarin in NSSPE was mainly related to the existing nanocrystal form which could promote dissolution, puerarin as well as Ligusticum chuanxiong oil which could promote drug transmembrane transport and inhibit drug efflux. It is the unique structure and composition of the compound NSSPE that promoted the oral absorption of puerarin.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210129.304DOI Listing
April 2021

Pexidartinib improves physical functioning and stiffness in patients with tenosynovial giant cell tumor: results from the ENLIVEN randomized clinical trial.

Acta Orthop 2021 Aug 12;92(4):493-499. Epub 2021 May 12.

Department of Orthopaedic Surgery, Memorial Sloan Kettering Cancer Center and Weill Cornell Medical College, New York, NY, USA.

Background and purpose - The ENLIVEN trial showed that, after 25 weeks, pexidartinib statistically significantly reduced tumor size more than placebo in patients with symptomatic, advanced tenosynovial giant cell tumor (TGCT) for whom surgery was not recommended. Here, we detail the effect of pexidartinib on patient-reported physical function and stiffness in ENLIVEN.Patients and methods - This was a planned analysis of patient-reported outcome data from ENLIVEN, a double-blinded, randomized phase 3 trial of adults with symptomatic, advanced TGCT treated with pexidartinib or placebo. Physical function was assessed using the Patient-Reported Outcomes Measurement Information System (PROMIS)-physical function (PF), and worst stiffness was assessed using a numerical rating scale (NRS). A mixed model for repeated measures was used to compare changes in PROMIS-PF and worst stiffness NRS scores from baseline to week 25 between treatment groups. Response rates for the PROMIS-PF and worst stiffness NRS at week 25 were calculated based on threshold estimates from reliable change index and anchor-based methods.Results - Between baseline and week 25, greater improvements in physical function and stiffness were experienced by patients receiving pexidartinib than patients receiving placebo (change in PROMIS-PF = 4.1 [95% confidence interval (CI) 1.8-6.3] vs. -0.9 [CI -3.0 to 1.2]; change in worst stiffness NRS = -2.5 [CI -3.0 to -1.9] vs. -0.3 [CI -0.9 to 0.3]). Patients receiving pexidartinib had higher response rates than patients receiving placebo for meaningful improvements in physical function and stiffness. Improvements were sustained after 50 weeks of pexidartinib treatment.Interpretation - Pexidartinib treatment provided sustained, meaningful improvements in physical function and stiffness for patients with symptomatic, advanced TGCT.
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http://dx.doi.org/10.1080/17453674.2021.1922161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382018PMC
August 2021
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