Publications by authors named "Xin Yang"

1,785 Publications

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Predicting prognosis in patients with stroke treated with intravenous alteplase through the 24-h trajectory of blood pressure changes.

J Clin Hypertens (Greenwich) 2021 Aug 4. Epub 2021 Aug 4.

Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatic Disease, Beijing, China.

Blood pressure (BP) monitored within 24 h from the beginning of intravenous thrombolysis (IVT) with alteplase, is one of the important factors affecting the prognosis of patients with acute ischemic stroke (AIS). This study aimed to explore longitudinal BP trajectory patterns and determine their association with stroke prognosis after thrombolysis. From November 2018 to September 2019, a total of 391 patients were enrolled consecutively during the study period, and 353 patients were ultimately analyzed. Five systolic (SBP) and four diastolic blood pressure (DBP) trajectory subgroups were identified. The regression analysis showed that when compared with the rapidly moderate stable group, the continuous fluctuation-very high level SBP group (odds ratio [OR]: 2.743, 95% confidence interval [CI]: 1.008-7.467) was associated with early neurological deterioration (END). Both the rapid drop-high level SBP (OR: 0.448, 95% CI: 0.219-0.919) and DBP groups (OR: 0.399, 95% CI: 0.219-0.727) were associated with early neurological improvement (ENI). Moreover, there was a U-shaped correlation between the OR value of SBP trajectory group and favorable outcome (the modified Rankin Scale [mRS] score 0-2) at 3 months: the slow drop-low level SBP group represent a well-established unfavorable outcome risk factor (OR:5.239, 95% CI: 1.271-21.595), and extremely high SBP-the continuous fluctuation-very high level SBP group, are equally associated with elevated unfavorable outcome risk (OR:3.797, 95% CI: 1.486-9.697). The continuous fluctuation-very high level DBP group was statistically significant in mRS (OR: 3.387, CI: 1.185-9.683). The BP trajectory groups show varying clinical features and risk of neurological dysfunction. The findings may help identify potential candidates for clinical BP monitoring, control, and specialized care.
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http://dx.doi.org/10.1111/jch.14331DOI Listing
August 2021

EHMT2 promotes the pathogenesis of hepatocellular carcinoma by epigenetically silencing APC expression.

Cell Biosci 2021 Aug 3;11(1):152. Epub 2021 Aug 3.

Organ Transplantation Center, The Affiliated Hospital of Qingdao University, 59 Haier Blvd, Qingdao, 266000, Shandong, China.

Background: Hepatocellular carcinoma (HCC), the second leading cause of cancer death worldwide, alone accounts for over half (466,100) of new cancer cases and 422,100 deaths based on the average year incidence rates of 2009 to 2011 in China. Due to unclear and complex underlying mechanisms for HCC development, effective therapy for HCC is still unavailable. The Wnt-β-catenin pathway is a critical contributor of HCC pathogenesis: 40-70% of HCCs from patients harbor the nuclear accumulation of β-catenin protein. However, the mechanisms for β-catenin activation are not fully understood.

Methods: The deletion of EHMT2 in Hep3B and Huh1 cells was achieved by transiently transfecting cells with pX459 plasmids, which carry EHMT2 specific small guide RNA (sgRNA) sequences for Cas9 protein. All experiments were performed in triplicate and repeated more than three times.

Results: In the present study, we observed that EHMT2 (but not EHMT1) mRNA and protein levels were significantly elevated in HCC compared with normal controls. Next, the results of Ki67 staining, as well as MTT, soft-agar and xenograft assays, in wild-type and EHMT2 Hep3B and Huh1 cancer stem cells collectively revealed that the elevation of EHMT2 expression is required for the tumorigenesis of HCC. Meanwhile, we found that elevated EHMT2 expression contributes to the activation of Wnt-β-catenin signaling: deletion of EHMT2 in Hep3B or Huh1 cells promoted the cytoplasmic localization of β-catenin and restrained the expression of Wnt-β-catenin signaling targets such as Myc, CCND1, MMP-7, etc. We demonstrated that EMHT2 directly mediates the H3K9me2 methylation of the APC promoter to epigenetically silence its expression. More intriguingly, our findings also showed that UNC0642, a specific inhibitor of EHMT2, exhibits anti-tumorigenesis effects in HCC both in vitro and in vivo, which were largely abolished by deletion of EHMT2 or overexpression of APC in Hep3B and Huh1 cells.

Conclusion: Altogether, our observations emphasize that the EHMT2-APC axis is a critical contributor to Wnt-β-catenin pathway activation in HCC, and UNC0642 may be a potential candidate for target drug treatment of HCC.
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http://dx.doi.org/10.1186/s13578-021-00663-9DOI Listing
August 2021

Identification and integrative analysis of ACLY and related gene panels associated with immune microenvironment reveal prognostic significance in hepatocellular carcinoma.

Cancer Cell Int 2021 Aug 3;21(1):409. Epub 2021 Aug 3.

Department of General Surgery, Huashan Hospital, Cancer Metastasis Institute, Fudan University, 12 Urumqi Road (M), Shanghai, 200040, China.

Background: Cumulating evidence reveals the key role of aberrant lipogenesis and immunogenomic features in hepatocellular carcinoma (HCC). However, there are still obstacles in our understanding of the complicated interaction between metabolic reprogramming and tumor immune microenvironment.

Methods: We compared metabolomic, transcriptomic and immunogenomic characteristics of portal vein tumor thrombosis (PVTT) and primary tumor to seek valuable markers. Human HCC samples with PVTT (n  =  28) was analyzed through ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS). Transcript levels of mRNA in two cohorts from published database GEO (n  =  60) and TCGA (n  =  411) were downloaded to explore differentially expressed genes and functional enriched gene set. Evaluation of immune infiltration was estimated and validated from transcriptomic data in both cohorts through six immune deconvolution algorithms and in a high-resolution mode (CIBERSORTx). Survival analysis (Kaplan-Meier and multivariable Cox regression model) was performed to examine prognostic value of ACLY, related immune checkpoints and immune infiltration levels from TCGA cohort. LASSO regression was further conducted to determine a gene panel to further predict survival outcomes associated with ACLY.

Results: We identified a novel signature, ATP citrate lyase, through transcriptomic and metabolomic approaches. We demonstrated that the metabolism adaptations in both fatty acid and cholesterol biosynthesis triggered by ACLY oncogenic activation. We illustrated the crucial function of ACLY in lipogenesis and its potential interaction with immune microenvironment. CD276, a promising target in immune checkpoint blockade, showed correlation to ACLY and differential expression in ACLY risk classification. Combination of ACLY, CD276 and immune infiltration level and a novel ACLY-associated panel from a predictive model retrieved from published database validated the prognostic value to risk stratification in patients with HCC.ACLY blockade to counteract metabolic activation and immunosuppressive status of the tumor microenvironment highlighted attractive prospect for translational application.

Conclusions: We investigated ACLY and its indispensable role in metabolism, immune function and a prognostic gene panel in HCC. We anticipate that the multifaced role of ACLY may reveal the potential value for mechanistic research and combinational therapy, suggesting that the combination blockade of ACLY and immune checkpoints may work as a promising strategy.
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http://dx.doi.org/10.1186/s12935-021-02108-2DOI Listing
August 2021

Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickens.

J Anim Sci Biotechnol 2021 Aug 3;12(1):86. Epub 2021 Aug 3.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

Background: Many researches about in ovo feeding (IOF) of vitamin C (VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems.

Results: Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase (GLO) expression was lower during pre-hatch development than that during post-hatch development. Sodium-dependent vitamin C transporter 1 (SVCT1) expression was increased continuously in yolk sac from embryonic age 19 (E19) to post-hatch day 1 (D1) and in intestine (duodenum, jejunum and ileum) from E17 to D1. Plasma VC content was higher at D1 than that at D21 and D42. IOF of VC significantly reduced GLO expression in liver, kidney and yolk sac as well as SVCT1 expression in duodenum, jejunum and ileum, but increased the VC content in plasma, brain, kidney and liver. In addition, IOF of VC obviously reduced the embryonic morality and increased the hatchability under heat stress.

Conclusions: This study suggested that IOF of VC at E11 in yolk was effective for embryonic VC supplementation. These findings provide a theoretical reference about the method of embryonic VC supplementation and effective methodology on embryonic VC nutrition in broiler chickens.
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http://dx.doi.org/10.1186/s40104-021-00607-wDOI Listing
August 2021

Filtration of Active Components with Antioxidant Activity Based on the Differing Antioxidant Abilities of and through UPLC/MS Coupling with Network Pharmacology.

Evid Based Complement Alternat Med 2021 21;2021:5547976. Epub 2021 Jul 21.

School of General Medicine and Continuing Education, Qiqihar Medical University, No. 333, Bukui Street, Qiqihar 161006, China.

This study attempted to filter active components with antioxidant activities based on the differing antioxidant abilities of () and (). First, the antioxidant activity of and was evaluated through the DPPH free radical scavenging method and compared with the half maximal inhibitory concentration (IC) value. Next, components of and were detected by employing ultrahigh-performance liquid chromatography-Q-Exactive Orbitrap mass spectrometry (UPLC-QEO/MS) technology, and differential compounds were screened out as potential antioxidant compounds by using Compound Discover 3.1 Software. After that step, in order to verify the antioxidant compounds, the network method was applied. Biological targets were searched in the GeneCards database, and that related to antioxidant ability were selected in the Comparative Toxicogenomics Database (CTD). Finally, the pharmacology network was constructed. Results showed that and possessed different compounds and antioxidant abilities. A total of 14 differential compounds such as -schizandrin, schisandrin B, schisandrin, and tigloylgomisin H between them were screened out and identified. Twenty targets associated with antioxidant activity contained MAP2K1, MAPK8, RPS6KB1, PRKCB, HIF1A, and so on were investigated. Thirty-six pathways contained HIF-1 signaling pathways, choline metabolism in cancer, serotonergic synapse, Fc epsilon RI signaling pathway, GnRH signaling pathway, and so on related to the above twenty targets were identified. The pharmacology network analysis indicated that the differential components may be helpful in treating various diseases, especially cancer, by exerting antioxidant activity. In conclusion, this study provided a novel method for identifying active components with antioxidant activity in and , and this method may be applicable for the filtration of bioactive components in other herbs.
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http://dx.doi.org/10.1155/2021/5547976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321731PMC
July 2021

Mussel-inspired triple bionic adsorbent: Facile preparation of layered double [email protected]@metal-polyphenol networks and their selective adsorption of dyes in single and binary systems.

J Hazard Mater 2021 Jul 9;420:126609. Epub 2021 Jul 9.

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality & Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs of China, 100081 Beijing, China. Electronic address:

To effectively address the serious human health challenges and ecological damage caused by organic dyes in wastewater, we developed a novel bionic adsorbent ([email protected]@MPNs) for the selective adsorption and removal of malachite green (MG) and crystalline violet (CV). The adsorbent was prepared using a facile two-step method based on mussel-inspired chemistry and metal complexation. The physicochemical structure, surface morphology, and composition of the [email protected]@MPNs were characterized by scanning electron microscopy, Fourier-transform infrared spectrometry, X-ray photoelectron spectroscopy, and thermogravimetric analysis. Adsorption of MG and CV with the [email protected]@MPNs was evaluated. Under optimal conditions, the maximum adsorption of MG and CV by the adsorbent was 89.608 and 40.481 mg/g, respectively. The adsorption kinetics showed that the experimental data were in good agreement with the pseudo-second-order kinetic model, and the equilibrium adsorption isotherm data fitted well with the Freundlich model. The thermodynamic results indicated that the adsorption of the dyes on [email protected]@MPNs was a spontaneous endothermic process. Importantly, the bionic adsorbent not only shows high removal efficiency by easy regeneration with low-cost reagents but also exhibits high selectivity for dyes in both single and binary systems. Therefore, [email protected]@MPNs have the potential to adsorb and remove dyes from complex wastewater solutions.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126609DOI Listing
July 2021

Incidence of multiple sclerosis in China: A nationwide hospital-based study.

Lancet Reg Health West Pac 2020 Aug 6;1:100010. Epub 2020 Aug 6.

China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Background: Multiple sclerosis (MS) is a leading cause of disability among young adults and effects considerable social and economic burdens. Data of MS incidence in China at the national level is lacking. We conducted the first nationwide hospital-based study to estimate the incidence and hospitalization burden of MS in China.

Methods: This study is based on an administrative database of the National Hospital Quality Monitoring System, which covers all 1665 tertiary hospitals in mainland China. The "Medical Record Homepage" of all patients, including 346 variables including demographic characteristics, diagnoses, procedures, and expenses etc., were uniformly collected across each tertiary hospital via standard protocol. MS was defined by the 2010 International Panel criteria for MS and was identified by ICD-10 code (G35•0).

Findings: We identified 27,336 hospital admissions for 15,060 MS patients from 2016 to 2018; amongst these patients, 9,879 were newly diagnosed. The age- and sex-adjusted incidence per 100,000 person-years is 0•235 (95% confidence interval [CI] 0•230-0•240), with 0•055 (0•050-0•060) in children and 0•288 (0•282-0•294) in adults, respectively. The female to male ratio is 2•02; the peak disease onset is age of 40-49 years. Residents in high-latitude and high-altitude areas are more likely to develop MS (F = 8•99; p < 0•001). Prevalent comorbidities include hypertension (18•8%), diabetes (7•2%), stroke (14•7%), depression or anxiety (3•7%), and autoimmune disease (2•3%). Through 2016-2018, 104 adults and 2 children died, with a hospital mortality rate of 9•9 per 1,000 person-years.

Interpretation: For the first time, we obtain the national incidence of MS as 0.055 in children and 0.288 in adults per 100,000 in China. The geographical distribution of MS incidence presented a north-south latitude gradient and a west-east altitude gradient.

Funding: National Science Foundation of China (81801199, 91642205, and 81830038); Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing.
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http://dx.doi.org/10.1016/j.lanwpc.2020.100010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315658PMC
August 2020

Assessment of Trends in Guideline-Based Oral Anticoagulant Prescription for Patients With Ischemic Stroke and Atrial Fibrillation in China.

JAMA Netw Open 2021 Jul 1;4(7):e2118816. Epub 2021 Jul 1.

China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Importance: Adherence to oral anticoagulants (OACs) per guideline recommendations is crucial in reducing ischemic stroke and systemic thromboembolism in high-risk patients with ischemic stroke and atrial fibrillation. However, data on OAC use are underreported in China.

Objective: To assess adherence to the Chinese Stroke Association or the American Heart Association/American Stroke Association's clinical management guideline-recommended prescription of OACs, the temporal improvement in adherence, and the risk factors associated with OAC prescriptions.

Design, Setting, And Participants: This quality improvement study was conducted at 1430 participating hospitals in the Chinese Stroke Center Alliance (CSCA) among patients with ischemic stroke and atrial fibrillation enrolled in the CSCA between August 1, 2015, and July 31, 2019.

Exposure: Calendar year.

Main Outcomes And Measures: Adherence to the Chinese Stroke Association or the American Heart Association/American Stroke Association's clinical management guideline-recommended prescribing of OACs (warfarin and non-vitamin K OACs, including dabigatran, rivaroxaban, apixaban, and edoxaban) at discharge.

Results: Among 35 767 patients (18 785 women [52.5%]; mean [SD] age, 75.5 [9.2] years) with previous atrial fibrillation at admission, the median CHA2DS2-VASc (cardiac failure or dysfunction, hypertension, age 65-74 [1 point] or ≥75 years [2 points], diabetes, and stroke, transient ischemic attack or thromboembolism [2 points]-vascular disease, and sex category [female]) score was 4.0 (interquartile range, 3.0-5.0); 6303 (17.6%) were taking OACs prior to hospitalization for stroke, a rate that increased from 14.3% (20 of 140) in the third quarter of 2015 to 21.1% (118 of 560) in the third quarter of 2019 (P < .001 for trend). Of 49 531 eligible patients (26 028 men [52.5%]; mean [SD] age, 73.4 [10.4] years), 20 390 (41.2%) had an OAC prescription at discharge, an increase from 23.2% (36 of 155) in the third quarter of 2015 to 47.1% (403 of 856) in the third quarter of 2019 (P < .001 for trend). Warfarin was the most commonly prescribed OAC (11 956 [24.2%]) and had the largest temporal increase (from 5.8% [9 of 155] to 20.7% [177 of 856]). Older age (adjusted odds ratio [aOR] per 5 year increase, 0.89;95% CI, 0.89-0.90), lower levels of education (aOR for below elementary school, 0.84; 95% CI, 0.74-0.95 ), lower income (aOR for ≤¥1000 [$154], 0.66; 95% CI, 0.59-0.73), having new rural cooperative medical scheme insurance (aOR, 0.92; 95% CI, 0.87-0.96), prior antiplatelet use (aOR, 0.70; 95% CI, 0.66-0.74), having several cardiovascular comorbid conditions (including stroke or transient ischemic attack [aOR, 0.78; 95% CI, 0.75-0.82], hypertension [aOR, 0.84; 95% CI, 0.80-0.89], diabetes [aOR, 0.91; 95% CI, 0.83-0.99], dyslipidemia [aOR, 0.87; 95% CI, 0.80-0.94], carotid stenosis [aOR, 0.83; 95% CI, 0.69-0.98], and peripheral vascular disease [aOR, 0.80; 95% CI, 0.71-0.90]), and admission to secondary hospitals (aOR, 0.71; 95% CI, 0.68-0.74) or hospitals located in the central region of China (aOR, 0.80; 95% CI, 0.75-0.84) were associated with not being prescribed an OAC at discharge.

Conclusions And Relevance: This quality improvement study suggests that, despite significant improvement over time, OAC prescriptions remained low. Efforts to increase OAC prescriptions, especially non-vitamin K OACs, are needed for vulnerable subgroups by age, socioeconomic status, and presence of comorbid conditions.
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http://dx.doi.org/10.1001/jamanetworkopen.2021.18816DOI Listing
July 2021

Microplastics in the environment: Sampling, pretreatment, analysis and occurrence based on current and newly-exploited chromatographic approaches.

Sci Total Environ 2021 Jun 29;794:148725. Epub 2021 Jun 29.

Laboratory of Environmental Pollution Control, Department of Chemistry, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Greece; Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, Thessaloniki GR-57001, Greece. Electronic address:

The omnipresent character of microplastics (MPs) in environmental matrices, organisms and products has recently posed the need of their qualitative as well as quantitative analysis imperative, in order to provide data about their abundance and specification of polymer types in several substrates. In this framework, current and emerging approaches based on the chromatographic separation are of increased relevance in the field of MPs analysis and possess a large number of merits, since most of them are applicable in various complex matrices, sensitive and ideal for the detection of small-sized particles, whereas the common absence of any special pre-treatment step before analysis should also be highlighted. Αnalytical pyrolysis coupled with gas chromatography mass spectrometry (GC-MS) has recently gained ground as a powerful means to deliver information on MPs composition and degradation after their release into environment. Several instrumentations and trends in the area of analytical pyrolysis are thoroughly described within this review, while newly-exploited chromatographic methods in the field of MPs analysis, including Liquid Chromatography (LC) and Gel Permeation Chromatography (GPC) in this line are also investigated. The present review fills the gap of standardization concerning sampling, pre-treatment and chromatographic approaches and gathers all the available methodologies applied inside this area in accordance with the studied substrate, with the most examined environmental matrices being the solid one. After investigating the various works, some development options arise and it appears that chromatographic approaches should focus on improved extraction processes in terms of MPs isolation, since it is a crucial part in plastic items monitoring and is commonly depended on the polymer type and matrix. Special attention is given on the potential of chromatographic techniques for microplastics identification as well as quantification by confirming the current research status and knowledge gaps and highlighting some of the recent trends in this field.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148725DOI Listing
June 2021

Breast and Prostate Cancer Risks for Male BRCA1 and BRCA2 Pathogenic Variant Carriers Using Polygenic Risk Scores.

J Natl Cancer Inst 2021 Jul 28. Epub 2021 Jul 28.

Department of Molecular Medicine, University La Sapienza, Rome, Italy.

Background: Recent population-based female breast cancer and prostate cancer polygenic risk scores (PRS) have been developed. We assessed the associations of these PRS with breast and prostate cancer risks for male BRCA1 and BRCA2 pathogenic variant carriers.

Methods: 483 BRCA1 and 1,318 BRCA2 European ancestry male carriers were available from the Consortium of Investigators of Modifiers of BRCA1/2 (CIMBA). A 147-single nucleotide polymorphism (SNP) prostate cancer PRS (PRSPC) and a 313-SNP breast cancer PRS were evaluated. There were three versions of the breast cancer PRS, optimized to predict overall (PRSBC), estrogen-receptor (ER) negative (PRSER-) or ER-positive (PRSER+) breast cancer risk.

Results: PRSER+ yielded the strongest association with breast cancer risk. The odds ratios (ORs) per PRSER+ standard deviation estimates were 1.40 (95% confidence interval [CI] =1.07-1.83) for BRCA1 and 1.33 (95% CI = 1.16-1.52) for BRCA2 carriers. PRSPC was associated with prostate cancer risk for both BRCA1 (OR = 1.73, 95% CI = 1.28-2.33) and BRCA2 (OR = 1.60, 95% CI = 1.34-1.91) carriers. The estimated breast cancer ORs were larger after adjusting for female relative breast cancer family history. By age 85 years, for BRCA2 carriers, the breast cancer risk varied from 7.7% to 18.4% and prostate cancer risk from 34.1% to 87.6% between the 5th and 95th percentiles of the PRS distributions.

Conclusions: Population-based prostate and female breast cancer PRS are associated with a wide range of absolute breast and prostate cancer risks for male BRCA1 and BRCA2 carriers. These findings warrant further investigation aimed at providing personalized cancer risks for male carriers and to inform clinical management.
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http://dx.doi.org/10.1093/jnci/djab147DOI Listing
July 2021

[Impact of proximal femoral shape on leg length discrepancy after total hip arthroplasty].

Zhongguo Gu Shang 2021 Jul;34(7):641-5

Department of Orthopaedics, Yijishan Hospital, Wannan Medical College, Wuhu 241000, Anhui, China.

Objective: To investigate the effect of different proximal femoral shapes on leg length discrepancy(LLD) after total hip arthroplasty(THA).

Methods: Total 131 patients with osteoarthritis or osteonecrosis received unilateral biological total hip arthroplasty from June 2013 to June 2019. All patients' age, sex, side and pelvis anteroposterior digital radiography were retraspectively analyzed. There were 69 males and 62 females, 57 cases of left hip and 74 cases of right hip. The age ranges from 25 to 89 with an average age of 62 years. There were 48 cases of osteoarthritis and 83 cases of osteonecrosis. In this study, femoral cortical index (FCI) was used as the classification of proximal femoral shape, and bilateral lower limb length differences were measured by preoperative and postoperative pelvis anteroposterior digital radiography. Grouping according to FCI:> 0.6 was Dorr A group, 0.5 to 0.6 was Dorr B group, <0.5 was the Dorr C group, and the relationship between proximal femur morphology and the leg length discrepancy after total hip arthroplasty was determined.

Results: The postoperative average FCI was(0.56±0.08) mm and the median LLD was 5.10 mm (IQR -1.00 to 8.80 mm). Three groups were divided according to the level of FCI, and there were no statistically significant differences in gender, age, side, diagnosis and intraoperative fracture rate distribution among three groups. FCI>0.6, the postoperative LLD was 6.30 mm (IQR 1.00 to 10.95 mm). When FCI was 0.5 to 0.6, the postoperative LLD was 5.85 mm(IQR-0.55 to 8.90 mm). FCI<0.5, the postoperative LLD was 1.95 mm(IQR -2.50 to 6.68 mm). LLD comparison of different proximal femoral shape was statistically significant (<0.05).

Conclusion: High FCI increases the risk of lower extremity prolongation after surgery on the affectedside, while low FCI reduces the risk of lower extremity prolongation after surgery on the affected side. The surgeon can assess the shape of the proximal femur of the patient preoperatively and inform the patient in advance of possible changes in leg length of both lower extremities after total hip replacement.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.07.010DOI Listing
July 2021

Follow-up study of airway microbiota in children with persistent wheezing.

Respir Res 2021 Jul 27;22(1):213. Epub 2021 Jul 27.

Department of Rheumatology Immunology & Allergy, and Pulmonary Medicine, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, People's Republic of China.

Background: Increasing evidence revealed that airway microbial dysbiosis was associated with increased risk of asthma, or persistent wheezing (PW). However, the role of lung microbiota in PW or wheezing recurrence remains poorly understood.

Methods: In this prospective observational study, we performed a longitudinal 16S rRNA-based microbiome survey on bronchoalveolar lavage (BAL) samples collected from 35 infants with PW and 28 age-matched infants (control group). A 2-year follow-up study on these PW patients was conducted. The compositions of lower airway microbiota were analyzed at the phylum and genus levels.

Results: Our study showed a clear difference in lower airway microbiota between PW children and the control group. Children with PW had a higher abundance of Elizabethkingia and Rothia, and lower abundance of Fusobacterium compared with the control group. At the end of the 2-year follow-up, 20 children with PW (57.1%) experienced at least one episode of wheezing, and 15 (42.9%) did not suffer from wheezing episodes. Furthermore, PW children with recurrence also had increased abundances of Elizabethkingia and Rothia relative to those who had no recurrence. Additionally, wheezing history, different gender, and caesarean section demonstrated a greater impact in airway microbiota compositions.

Conclusion: This study suggests that the alterations of lower airway microbiota could be strongly associated with the development of wheezing, and early airway microbial changes could also be associated with wheezing recurrence later in life.
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http://dx.doi.org/10.1186/s12931-021-01806-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314579PMC
July 2021

Phytonanomaterials as therapeutic agents and drug delivery carriers.

Adv Drug Deliv Rev 2021 Jul 22:113868. Epub 2021 Jul 22.

Department of Neurosurgery, Yale University, New Haven, CT, 06510, US; Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225009, China. Electronic address:

Medicinal plants have been a major resource for drug discovery. Emerging evidence shows that in addition to pharmacologically active components, medicinal plants also contain phytochemical nanomaterials, or phytonanomaterials, which form nanoparticles for drug delivery. In this review, we examine the evidence supporting the existence of phytonanomaterials. Next, we review identification, isolation, and classification of phytonanomaterials, characteristics of phytonanomaterial-derived nanoparticles, and molecular mechanisms of phytonanomaterial assembly. We will then summarize the current progress in exploring phytonanomaterial-derived NPs as therapeutic agents and drug delivery carriers for disease treatment. Last, we will provide perspectives on future discovery and applications of phytonanomaterials.
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http://dx.doi.org/10.1016/j.addr.2021.113868DOI Listing
July 2021

Acetazolamide Alleviate Cerebral Edema Induced by Ischemic Stroke Through Inhibiting the Expression of AQP4 mRNA.

Neurocrit Care 2021 Jul 23. Epub 2021 Jul 23.

Department of Neurosurgery, The First Hospital of Shanxi Medical University, 85 Jiefang South Road, Taiyuan, Shanxi, People's Republic of China.

Objective: We want to investigate the effect of aquaporin-4 (AQP4) on cerebral edema induced by ischemic stroke in rats and explore whether inhibiting the expression of AQP4 through acetazolamide (AZA) could attenuate brain edema and protect cerebral function.

Methods: The Sprague Dawley (SD) rats were randomly divided into four groups: sham + saline group, sham + AZA group, AZA intervention group, and nonintervention group. Each group was divided into five subgroups according to the time of cerebral ischemia (6 h, 1 day, 3 days, 5 days, and 7 days). The model of cerebral infarction in rats was adopted by means of the bilateral carotid arteries ligation (2-VO) method. The rats in intervention group were given intraperitoneal injection of AZA (35 mg/kg/day). Hematoxylin-eosin staining was performed for pathological analysis of the infarcted area. The brain water content was calculated to evaluate the degree of brain edema. The messenger RNA (mRNA) and protein expressions of AQP4 in the brain were measured by quantitative real-time polymerase chain reaction and immunohistochemistry, respectively.

Results: Significant cerebral pathological damages were found in ischemic stroke rats. The brain water content, protein, and mRNA expression of AQP4 of the intervention and nonintervention groups were markedly higher than those of the sham groups. By contrast, AZA administration reduced the brain water content, whereas improved cerebral dysfunction was induced by ischemic stroke. Moreover, AZA obviously reduced the protein and mRNA expression of AQP4 after ischemic stroke in rats' brains.

Conclusions: The expression of AQP4 was closely related to cerebral edema induced by ischemic stroke. Decreasing the expression of AQP4 mRNA by AZA administration can effectively relieve cerebral edema and decrease cerebral pathological damage.
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http://dx.doi.org/10.1007/s12028-021-01261-wDOI Listing
July 2021

Scalable High-Pressure Synthesis of sp-sp Carbon Nanoribbon via [4 + 2] Polymerization of 1,3,5-Triethynylbenzene.

J Phys Chem Lett 2021 Aug 23;12(30):7140-7145. Epub 2021 Jul 23.

Center for High Pressure Science and Technology Advanced Research, 10 Xibeiwang East Road, Haidian, Beijing 100094, China.

Pressure-induced polymerization of aromatics is an effective method to construct extended carbon materials, including the diamond-like nanothread and graphitic structures, but the reaction pressure of phenyl is typically around 20 GPa and too high to be applied for large-scale preparation. Here by introducing ethynyl to phenyl, we obtained a sp-sp carbon nanoribbon structure by compressing 1,3,5-triethynylbenzene (TEB), and the reaction pressure of phenyl was successfully decreased to 4 GPa, which is the lowest reaction pressure of aromatics at room temperature. Using experimental and theoretical methods, we figured out that the ethynylphenyl of TEB undergoes [4 + 2] dehydro-Diels-Alder (DDA) reaction with phenyl upon compression at an intermolecular C···C distance above 3.3 Å, which is much longer than those of benzene and acetylene. Our research suggested that the DDA reaction between ethynylphenyl and phenyl is a promising route to decrease the reaction pressure of aromatics, which allows the scalable high-pressure synthesis of nanoribbon materials.
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http://dx.doi.org/10.1021/acs.jpclett.1c01945DOI Listing
August 2021

Rate Constants and Mechanisms for Reactions of Bromine Radicals with Trace Organic Contaminants.

Environ Sci Technol 2021 Aug 23;55(15):10502-10513. Epub 2021 Jul 23.

School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, China.

Bromine radicals can pose great impacts on the photochemical transformation of trace organic contaminants in natural and engineered waters. However, the reaction kinetics and mechanisms involved are barely known. In this work, second-order reaction rate constants with Br and Br were determined for 70 common trace organic contaminants and for 17 model compounds using laser flash photolysis and steady-state competition kinetics. The values ranged from <10 to (2.86 ± 0.31) × 10 M s and the values from <10 to (1.18 ± 0.09) × 10 M s at pH 7.0. Six quantitative structure-activity relationships were developed, which allow predicting additional unknown and values. Single-electron transfer was shown to be a favored pathway for the reactions of Br and Br with trace organic contaminants, and this was supported by transient spectroscopy and quantum chemical calculations. This study is essential in advancing the scientific understanding of halogen radical-involved chemistry in contaminant transformation.
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http://dx.doi.org/10.1021/acs.est.1c02313DOI Listing
August 2021

A Promising Anticancer Agent Dimethoxycurcumin: Aspects of Pharmacokinetics, Efficacy, Mechanism, and Nanoformulation for Drug Delivery.

Front Pharmacol 2021 6;12:665387. Epub 2021 Jul 6.

School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University) Ministry of Education, Yantai University, Yantai, China.

Curcumin is a well-known anticancer natural product with various significant bioactivities that has been well documented, but its widespread use is mainly hindered by insufficient ADME properties such as poor solubility and low metabolic stability. Dimethoxycurcumin (DiMC) is a kind of lipophilic compound derived from curcumin that maintains its anticancer potency and has greatly improved systematic bioavailability. Therefore, DiMC is regarded as a promising plant-derived anticancer agent that deserves to be well developed. Herein, we concentrate on the published work by those from original research groups concerned with the pharmacokinetics, efficacy, and mechanism of DiMC involved in the treatment of various tumors, as well as the nanoformulations for effective drug delivery.
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http://dx.doi.org/10.3389/fphar.2021.665387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290316PMC
July 2021

Identification of a Prognostic Index Based on a Metabolic-Genomic Landscape Analysis of Hepatocellular Carcinoma (HCC).

Cancer Manag Res 2021 15;13:5683-5698. Epub 2021 Jul 15.

Department of Infectious Diseases, The First Affiliated Hospital of University of South China, Heng Yang, Hunan, 421000, People's Republic of China.

Background: Metabolic disorders have attracted increasing attention from scientists who conduct research on various tumours, especially hepatocellular carcinoma (HCC). The purpose of this study was to assess the prognostic significance of metabolism in HCC.

Methods: The expression profiles of metabolism-related genes (MRGs) of 349 surviving HCC patients were extracted from The Cancer Genome Atlas (TCGA) database. Subsequently, a series of biomedical computational algorithms were used to identify a seven-MRG signature as a prognostic model. GSEA indicated the function and pathway enrichment of these MRGs. Then, drug sensitivity analysis was used to identify the hub gene, which was tested using IHC staining.

Results: A total of 420 differential MRGs and 116 differentially expressed transcription factors (TFs) were identified in HCC patients based on data from the TCGA database. The GO and KEGG enrichment analyses indicated that metabolic disturbance might be involved in the development of HCC. LASSO regression analysis was used to construct a seven-MRG signature (DHDH, ENO1, G6PD, LPCAT1, PDE6D, PIGU and PPAT) that could predict the prognosis of HCC patients. GSEA revealed the functional and pathway enrichment of these seven MRGs. Then, drug sensitivity analysis indicated that G6PD might play a key role in the prognosis of HCC by promoting chemoresistance. Finally, we used IHC staining to demonstrate the relationship between G6PD expression levels and clinical parameters in HCC patients.

Conclusion: The results of this study provide a potential method for predicting the prognosis of HCC patients and avenues for further studies of HCC metabolism. Moreover, the function of G6PD may play a key role in the development and progression of HCC.
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http://dx.doi.org/10.2147/CMAR.S316588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290353PMC
July 2021

Glutathione S-transferase A2 promotes hepatocellular carcinoma recurrence after liver transplantation through modulating reactive oxygen species metabolism.

Cell Death Discov 2021 Jul 21;7(1):188. Epub 2021 Jul 21.

Department of Surgery, HKU-SZH & LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.

Hepatocellular carcinoma (HCC) recurrence after liver transplantation remains a significant clinical problem. Ischemia-reperfusion injury (IRI) occurred inevitably at the early phase after liver transplantation (LT) spawns a significant risk of HCC recurrence. However, their linkage and IRI-derived risk factors for HCC recurrence remain exclusive. Understanding the mechanism of post-transplantation hepatic injury could provide new strategies to prevent the later event of HCC recurrence. We demonstrated that glutathione S-transferase A2 (GSTA2) expression was significantly associated with early phase hepatic and systemic injury and ROS level after liver transplantation. Early phase circulating GSTA2 (GSTA2) protein was a significant predictor of HCC recurrence and survival. Heterogeneous single nucleotide polymorphism at G335C of GSTA2 was significantly associated with poor survival of HCC recipients. Enhancement of GSTA2 could protect HCC cells against HO-induced cell death by compensating for the elevated ROS stress. We also demonstrated that GSTA2 played crucial roles in regulating the ROS-associated JNK and AKT signaling pathways and ROS metabolism in HCCs in responding to a dynamic ROS environment. Functionally, endogenous or exogenous upregulation of GSTA2 could promote HCC growth and invasion through activating the epithelial-mesenchymal-transition process. Targeted inhibition of GSTA2 could suppress HCC growth and metastasis. In conclusion, GSTA2 could be a novel prognostic and therapeutic target to combat HCC recurrence after liver transplantation.
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http://dx.doi.org/10.1038/s41420-021-00569-yDOI Listing
July 2021

Digital Light Processing 4D Printing of Transparent, Strong, Highly Conductive Hydrogels.

ACS Appl Mater Interfaces 2021 Aug 20;13(30):36286-36294. Epub 2021 Jul 20.

Key Laboratory of Science and Technology on High-tech Polymer Materials, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.

Hydrogels have excellent biocompatibility, transparency, stretchability, and ionic conductivity, but their fabrication through photopolymerization-based 3D printing is limited due to the low solubility of hydrophobic photoinitiators and lack of efficient hydrophilic photoinitiators. Herein, a type of microemulsion is synthesized and the common hydrophobic photoinitiator can be adopted and finally, a series of transparent hydrogels with high strength (up to 22.9 MPa), elasticity (up to 583%), and ionic conductivity (up to 9.64 S m) are fabricated through digital light processing 3D printing technology. Objects with complex structures and a high printing resolution are printed. Hydrogels with both high strength and high ionic conductivity are obtained through chemical crosslinking and ion coordination effect. Dual-material 3D printing is applied to package the hydrogel with elastomers. Due to the high sensitivity and reliability under both stretching and compressive deformation, the hydrogel sensors are applied to monitor various human motions. In addition, the hydrogel exhibits solvent-induced dehydration and excellent water-activated shape memory properties, which are greatly beneficial for its storage and applications in the biomedical field.
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http://dx.doi.org/10.1021/acsami.1c08438DOI Listing
August 2021

Preparation and Application of Quaternized Chitosan- and AgNPs-Base Synergistic Antibacterial Hydrogel for Burn Wound Healing.

Molecules 2021 Jul 1;26(13). Epub 2021 Jul 1.

Shenzhen Bao'an Traditional Chinese Medicine Hospital Group, Shenzhen 518133, China.

Infection is the major reason that people die from burns; however, traditional medical dressings such as gauze cannot restrain bacterial growth and enhance the healing process. Herein, an organic- and inorganic-base hydrogel with antibacterial activities was designed and prepared to treat burn wounds. Oxidized dextran (ODex) and adipic dihydrazide grafted hyaluronic acid (HA-ADH) were prepared, mixed with quaternized chitosan (HACC) and silver nanoparticles to fabricate [email protected]/HA-ADH/HACC hydrogel. The hydrogel, composed of nature biomaterials, has a good cytocompatibility and biodegradability. Moreover, the hydrogel has an excellent antibacterial ability and presents fast healing for burn wounds compared with commercial Ag dressings. The [email protected]/HA-ADH/HACC hydrogel will be a promising wound dressing to repair burn wounds and will significantly decrease the possibility of bacterial infection.
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http://dx.doi.org/10.3390/molecules26134037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271850PMC
July 2021

Bis-acenaphthoquinone diimides with high electron deficiency and good coplanar conformation.

Chem Commun (Camb) 2021 Aug;57(63):7822-7825

Laboratory of Molecular Materials and Devices, Department of Materials Science, Fudan University, Shanghai 200438, China.

A series of novel bis-acenaphthoquinone diimides featuring a highly electron-deficient bis-acenaphthoquinone core are facilely synthesized via Knoevenagel condensation reaction. The diimides show high electron deficiency and good coplanar conformation, together with one of them having a maximum electron mobility up to 0.038 cm2 V-1 s-1.
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http://dx.doi.org/10.1039/d1cc02693cDOI Listing
August 2021

The condensed tannin chemistry and astringency properties of fifteen Foex grapes and wines.

Food Chem X 2021 Oct 29;11:100125. Epub 2021 Jun 29.

College of Enology, Northwest A & F University, Yangling, Shaanxi 712100, China.

This study sought to determine the effects of variety on the astringency and chemistry of condensed tannins of spine grapes and wines. Fifteen varieties of red spine grape ( Foex) were used. Condensed tannin content, composition, and wine astringency were determined. The condensed tannin profiles were assessed by high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). The condensed tannin content highly depended on the variety ranging from 0.30 mg/g to 7.80 mg/g (in skins), from 3.12 mg/g to 8.82 mg/g (in seeds), and from 62.60 mg/L to 225.90 mg/L (in wines). There were significant differences in proportions of certain constitutive subunits (as mole%) and mean degree of polymerization (mDp) among the varieties. Correlation analysis revealed that condensed tannin concentration and composition had a significant effect on the sensory evaluation and quantitative analysis of astringency. A positive correlation between mDp and astringency was also observed. The present results expand knowledge of the characterization of spine grape and wine condensed tannin chemistry and astringency.
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http://dx.doi.org/10.1016/j.fochx.2021.100125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261001PMC
October 2021

Intravenous thrombolysis in Chinese patients with mild acute ischemic stroke.

Ann Transl Med 2021 May;9(9):767

Vascular Neurology, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Mild stroke accounts for more than a half of all stroke patients, and short-term outcomes after treatment with intravenous (IV) recombinant tissue plasminogen activator (rtPA) have not been fully investigated in this group.

Methods: Our study investigated short-term outcomes and predictors for a favorable functional outcome at discharge in mild stroke patients with IV rtPA. 6,752 mild stroke patients in the China Stroke Center Alliance with a clinical diagnosis of acute ischemic stroke, within 4.5 hours from symptom onset, with a baseline National Institutes of Health Stroke Scale score ≤5 and received rt-PA treatment were included in this retrospective analysis. Univariable and multivariable analyses were performed to identify factors independently associated with a favorable functional outcome.

Results: Only 18.5% had an unfavorable functional outcome at discharge, 91.1% were discharged home, 89.9% could ambulate independently, 95.9% had a length of stay of 3 days or longer and 1.9% had sICH. A multivariable Logistic regression model identified that age >80 years [adjusted odds ratio (aOR): 1.57 (1.1-2.25)], diabetes mellitus [aOR: 1.35 (1.16-1.58)], 3-4.5 h time window [aOR: 1.43 (1.26-1.63)] and NIHSS score [3 0, aOR: 1.49 (1.05-2.11); 4 0, aOR: 2.36 (1.68-3.33); 5 0, aOR: 2.51 (1.77-3.56)] were independent risk factors for mRS >2 with hospital region, hospital level and hypertension as covariates.

Conclusions: Our findings suggest that tPA is safe and effective in mild stroke patients with age ≤80 within the 3 hour time window and in those without diabetes mellitus, further studies are needed to confirm the findings.
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http://dx.doi.org/10.21037/atm-21-40DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246215PMC
May 2021

Zn-Ion Batteries: Boosting the Rate Capability and Low-temperature Performance by Combining Structure and Morphology Engineering.

ACS Appl Mater Interfaces 2021 Jul 14;13(29):34468-34476. Epub 2021 Jul 14.

College of Chemistry & Engineering, Yantai University, Yantai 264005, China.

Prussian blue analogues (PBAs) have been considered as one kind of the most promising cathode materials for Zn-ion batteries (ZIBs) due to their low cost, high performance, high safety, and high abundance. However, owing to the low conductivity and single electron reaction, it is a great challenge to obtain a PBA cathode material with high reversible capacity, high rate capability, and good temperature adaptability. Here, a cathode material, K(VO)[Fe(CN)]·6.8HO (KVHCF), with a multielectron reaction and double conductive carbon framework (DCCF) is designed and synthesized by combining structure and morphology engineering. With the multielectron reaction and high electronic conductivity simultaneously, the [email protected] cathode material delivers a high specific capacity (180 mAh·g @ 400 mA·g) and the best rate performance (116 mAh·g @ 8000 mA·g) of the reported PBAs. Moreover, [email protected] presents a high specific capacity of 132 mAh·g @ 400 mA·g at 0 °C. Even at -10 °C, it still delivers specific capacities of 127 mAh·g @ 40 mA·g and 80 mAh·g @ 400 mA·g with a retention of 86% after 700 cycles. In situ X-ray diffraction (XRD) and ex situ X-ray photoelectron spectroscopy (XPS) are carried out to investigate the charge-discharge electrochemical reaction mechanism.
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http://dx.doi.org/10.1021/acsami.1c09798DOI Listing
July 2021

Particulate matter exposure is highly correlated to pediatric asthma exacerbation.

Aging (Albany NY) 2021 07 13;13(13):17818-17829. Epub 2021 Jul 13.

Department of Pulmonology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou 310052, China.

Asthma is a heterogeneous disease in which environmental factors play an important role, and the effect of particulate matter (PM) on the occurrence and severity of asthma is drawing more attention. This study aims to identify the correlation between PM and pediatric asthma exacerbation and explore the potential mechanisms. The asthma visits data (N = 16,779,739) in a university-based tertiary children's hospital from January 2013 to December 2017 were collected, and the relationship between asthma visits and local PM concentration was analyzed. For further study, we established a house dust mite (HDM)-induced allergic airway inflammation model with PM intervention. We detected a correlation between PM concentration and pediatric asthma visits, especially in children under 6 years old. The data showed that PM aggravated HDM-induced airway inflammation, and IL-33 neutralizing antibody exerted a protective role. Our study suggests that PM is a risk factor in promoting pediatric asthma exacerbation, in which IL-33 might be a promising target.
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http://dx.doi.org/10.18632/aging.203281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312457PMC
July 2021

Major pathologic response assessment and clinical significance of metastatic lymph nodes after neoadjuvant therapy for non-small cell lung cancer.

Mod Pathol 2021 Jul 12. Epub 2021 Jul 12.

Department of Pathology, Peking University Cancer Hospital & Institute, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing, People's Republic of China.

For neoadjuvant therapy in patients with non-small cell lung cancer, the major pathologic response of primary tumors may be an assessable and reliable surrogate measure of survival. Few studies have examined the pathologic evaluation of metastatic lymph node responses and their prognostic significance. This retrospective study enrolled 336 patients with non-small cell lung cancer (squamous cell carcinoma, n = 216; adenocarcinoma, n = 120) treated with neoadjuvant therapy including chemotherapy (n = 316) and targeted therapy (adenocarcinoma, n = 20). The treatment response of the primary tumor and lymph node metastases (LNM) were pathologically assessed according to the multidisciplinary recommendations of the International Association for the Study of Lung Cancer. The relationship of overall survival (OS) and disease-free survival (DFS) with the responses of the primary tumor or LNM was analyzed. The optimal cutoff value of the residual viable tumor (%RVT) of the primary tumor was 12% for both OS (P < 0.001) and DFS (P < 0.001). The pathologic assessment identified LNM in 208 patients. The optimal %RVT cutoff value in LNM was 8% for both OS (P = 0.003) and DFS (P < 0.001). The Spearman's rank correlation coefficient between primary tumors and corresponding LNM was 0.487 for %RVT (P < 0.001), which indicated a positive correlation. On multivariable analysis, an RVT of the primary tumor ≤12% was an independent prognostic factor for improved OS (P = 0.024), whereas an RVT of LNM ≤ 8% was an independent prognostic factor for increased DFS (P = 0.018). Furthermore, in the neoadjuvant chemotherapy group, the optimal %RVT cutoff values for OS in patients with squamous cell carcinoma and adenocarcinoma in the primary tumor were 12% and 58%, respectively. Considering its convenience and operability in clinical application, a 10% threshold RVT value can be used for prognostic evaluation of LNM and primary tumors of squamous cell carcinoma histology; further studies are needed to confirm the optimal cutoff value for primary tumors of adenocarcinoma.
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http://dx.doi.org/10.1038/s41379-021-00871-1DOI Listing
July 2021

Contrastive rendering with semi-supervised learning for ovary and follicle segmentation from 3D ultrasound.

Med Image Anal 2021 Jun 22;73:102134. Epub 2021 Jun 22.

School of Biomedical Engineering, Health Center, Shenzhen University, China; Medical UltraSound Image Computing (MUSIC) Lab, Shenzhen University, China. Electronic address:

Segmentation of ovary and follicles from 3D ultrasound (US) is the crucial technique of measurement tools for female infertility diagnosis. Since manual segmentation is time-consuming and operator-dependent, an accurate and fast segmentation method is highly demanded. However, it is challenging for current deep-learning based methods to segment ovary and follicles precisely due to ambiguous boundaries and insufficient annotations. In this paper, we propose a contrastive rendering (C-Rend) framework to segment ovary and follicles with detail-refined boundaries. Furthermore, we incorporate the proposed C-Rend with a semi-supervised learning (SSL) framework, leveraging unlabeled data for better performance. Highlights of this paper include: (1) A rendering task is performed to estimate boundary accurately via enriched feature representation learning. (2) Point-wise contrastive learning is proposed to enhance the similarity of intra-class points and contrastively decrease the similarity of inter-class points. (3) The C-Rend plays a complementary role for the SSL framework in uncertainty-aware learning, which could provide reliable supervision information and achieve superior segmentation performance. Through extensive validation on large in-house datasets with partial annotations, our method outperforms state-of-the-art methods in various evaluation metrics for both the ovary and follicles.
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http://dx.doi.org/10.1016/j.media.2021.102134DOI Listing
June 2021

Fabrication of [email protected] microspheres by one-step electrospray for magnetic resonance imaging during transcatheter arterial embolization.

Acta Biomater 2021 Jul 7. Epub 2021 Jul 7.

National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, China. Electronic address:

Magnetic resonance imaging (MRI) has attracted increasing attention as a feasible alternative or adjunctive imaging modality for X-ray digital subtraction angiography because of the high tissue resolution and non-ionization radiation. In this study, a one-step electrospray method was developed to fabricate PVA microspheres encapsulated with in situ synthesized FeO nanoparticles. [email protected] microspheres were mono-dispersed black spheres with a wide range of sizes (262-958 µm). The in situ-synthesized FeO nanoparticles were used as the contrast agent of MRI and the cross-linkers of PVA matrixes for the embolization purpose. In vivo evaluation of renal arteries of normal rabbits showed that [email protected] microspheres had good embolic effect and enhanced capability of MRI. In vitro and in vivo biosafety assessment confirmed that [email protected] microspheres had favorable biocompatibility. The DOX-loaded [email protected] microspheres showed a typical drug-sustained release profile. These results suggest that the prepared DOX-loaded [email protected] microspheres have the function of MRI, embolotherapy and chemotherapy. We expect our study could provide a simple and useful approach for the systematic design, fabrication, and application of a new type of magnetic microspheres as a triple-functional embolic agent for the development of MRI-guided TACE. STATEMENT OF SIGNIFICANCE: Due to the low tissue resolution and hazardous ionization radiation of X-ray digital subtraction angiography, it is beneficial to study MR imaging embolic microspheres for the development of MRI-guided TACE. In this study, a one-step electrospray method was firstly developed to fabricate PVA microspheres encapsulated with in situ synthesized FeO nanoparticles. Then, chemotherapeutic agent (DOX), contrast media of MRI (FeO) and embolic agent (PVA matrix) were combined together in one body (DOX-loaded [email protected] microspheres) to achieve the triple effects of chemotherapy, MR imaging and embolization. This triple-functional embolic agent offers potential for the future development of MRI-guided TACE.
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http://dx.doi.org/10.1016/j.actbio.2021.07.006DOI Listing
July 2021

Knockdown of UGT352A5 decreases the thiamethoxam resistance in Bemisia tabaci (Hemiptera: Gennadius).

Int J Biol Macromol 2021 Jul 7;186:100-108. Epub 2021 Jul 7.

Hubei Engineering Technology Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, PR China; Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address:

Uridine diphosphate (UDP)-glycosyltransferases (UGTs), which are major phase II detoxification enzymes, have been implicated in the glycosylation of lipophilic endobiotics and xenobiotics and thus potentially lead to the evolution of insecticide resistance. In this study, we identified and cloned two putative UGT genes from transcriptome data which are named UGT352A4 and UGT352A5. As demonstrated by qRT-PCR, two UGT genes were over-expressed in the thiamethoxam-resistant (THQR) strain relative to the susceptible (THQS) strain. Moreover, the induction experiment revealed that the expression of the UGT352A5 gene was significantly increased following exposure to thiamethoxam in the THQR strain. Furthermore, the expression of both UGT352A4 and UGT352A5 was downregulated after RNA interference, whereas only the silencing of UGT352A5 resulted in a noticeable increase in the mortality of THQR adults. Our results represent the first line of evidence showing that UGT352A5 might be responsible for conferring thiamethoxam resistance in B. tabaci. The results will be shed new insights for obtaining a better understanding of the role of UGTs in the evolution of insecticide resistance and developing new insect resistance management tactics within the sustainable integrated pest management framework.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.040DOI Listing
July 2021
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