Publications by authors named "Xin Xin"

130 Publications

Clinical significance of kinesin family member 2A as a facilitating biomarker of disease surveillance and prognostication in cervical cancer patients.

Ir J Med Sci 2021 Apr 2. Epub 2021 Apr 2.

Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, 7th Floor, Renji Building, No. 26 Shengli Street, Jiang'an District, Wuhan, 430000, China.

Background: Cervical cancer is one of the most common and fatal malignancies among females, and biomarkers are essential for assisting in its management. Kinesin family member 2A (KIF2A) has been exhibited to be a potential maker in various cancers; however, its role in cervical cancer has yet to be reported. Therefore, we aimed to assess the expression of KIF2A and its correlation with clinicopathological characteristics as well as survival profile in cervical cancer patients.

Methods: A hundred and thirty-five cervical cancer patients who underwent simple trachelectomy or radical hysterectomy were retrospectively analyzed. Tumor tissues and paired adjacent tissues were acquired, in which KIF2A mRNA and protein expressions were determined by reverse transcription quantitative polymerase chain reaction and immunohistochemistry assay, respectively. Disease-free survival (DFS) and overall survival (OS) were documented with a median follow-up duration of 28.0 months.

Results: KIF2A protein (P < 0.001) and mRNA (P < 0.001) expressions were both upregulated in tumor tissues compared to paired adjacent tissues in cervical cancer patients. In addition, tumor tissue KIF2A protein and mRNA expressions were positively associated with lymph node metastasis (P = 0.025 and P = 0.010, respectively) and FIGO stage (P = 0.022 and P = 0.015, respectively) in cervical cancer patients. Moreover, patients with tumor tissue KIF2A high expression (mRNA and protein) displayed worse DFS (P = 0.010 and P = 0.046, respectively) and OS (P = 0.042 and P = 0.030, respectively) compared to patients with tumor tissue KIF2A low expression (mRNA and protein).

Conclusion: Tumor tissue KIF2A expression could serve as a biomarker enhancing the disease surveillance and prognostication in cervical cancer management.
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http://dx.doi.org/10.1007/s11845-021-02510-9DOI Listing
April 2021

Performances of simultaneous enhanced removal of nitrogen and phosphorus via biological aerated filter with biochar as fillers under low dissolved oxygen for digested swine wastewater treatment.

Bioprocess Biosyst Eng 2021 Apr 1. Epub 2021 Apr 1.

College of Resources and Environment, Chengdu University of Information Technology, Chengdu, 610225, People's Republic of China.

This study aims to explore the feasibility of biochar as a carrier to improve the simultaneous removal of nitrogen and phosphorus in biological aerated filters (BAFs) for treating low C/N digested swine wastewater (DSW). Two similar BAFs (BAF-A with hydrophobic polypropylene resin as fillers and BAF-B with bamboo biochar as carrier) were developed for DSW treatment. Results showed that the NH-N, TN, and TP removal performances in BAF-B were higher than those in BAF-A. Carrier type had an obvious influence on the structures and diversity of the microbial population. The biochar carrier in BAF-B was conducive to the enrichment of the functional microorganisms and the increase of microbial diversity under high NH-N conditions. Microbial analysis showed that the genera Rhodanobacter (10.64%), JGI_0001001-h003 (14.24%), RBG-13-54-9 (8.87%), Chujaibacter (11.27%), and Ottowia were the predominant populations involved in nitrogen and phosphorus removal in the later stage of phase III in BAF-B. BAF with biochar as carrier was highly promising for TN and TP removal in low C/N and high NH-N DSW treatment.
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http://dx.doi.org/10.1007/s00449-021-02557-zDOI Listing
April 2021

Imaging characteristics of a rare case of monostotic fibrous dysplasia of the sacrum: A case report.

World J Clin Cases 2021 Feb;9(5):1111-1118

MRI Department, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, Shaanxi Province, China.

Background: Fibrous dysplasia (FD) is a common benign intramedullary fibro-osseous lesion. Involvement of the spine is rare, with the literature including only case reports, and cases of monostotic FD (MFD) in the sacrum are extremely rare. A correct preoperative diagnosis of spinal MFD is important for clinicians to select proper treatment.

Case Summary: We retrospectively assessed a case report of MFD in the sacrum. This patient was examined by computed tomography (CT) and magnetic resonance imaging (MRI), and the diagnosis was confirmed by pathology. A review of the literature was performed to analyze the imaging characteristics and differential diagnoses of spinal MFD. For our patient, the CT scan showed the lesion to be expansile, with ground glass opacity and a sclerotic rim. On MRI, the lesion showed iso-low signal intensity on T1WI and iso-high signal intensity on T2WI. A low signal rim was found on T1WI and T2WI. Our patient was treated by posterior focal excision, decompression, bone grafting, fusion and pedicle screw fixation. A satisfactory result was achieved, with pain disappearance. No complications had occurred at the 1-year follow up.

Conclusion: MFD is an expansile osteolytic change. Ground glass opacity and a sclerotic margin are obvious characteristics. The lesion often involves the vertebral body and posterior element. Knowledge of these imaging characteristics of spinal FD could be helpful for diagnosis and prevent unnecessary procedures.
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http://dx.doi.org/10.12998/wjcc.v9.i5.1111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896654PMC
February 2021

Challenges and opportunities for ovarian cancer management in the epidemic of Covid-19: lessons learned from Wuhan, China.

J Ovarian Res 2021 Feb 18;14(1):35. Epub 2021 Feb 18.

Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, No. 26 Shengli Street, Jiangan District, Wuhan, 430014, China.

China and the rest of the world are experiencing an outbreak of the 2019 novel coronavirus disease (COVID-19). Patients with cancer are more susceptible to viral infection and are more likely to develop severe complications, as compared to healthy individuals. The growing spread of COVID-19 presents challenges for the clinical care of patients with gynecological malignancies. Ovarian debulking surgery combined with the frequent need for chemotherapy is most likely why ovarian cancer was rated as the gynecologic cancer most affected by COVID-19. Therefore, ovarian cancer presents a particular challenging task. Concerning the ovarian cancer studies with confirmed COVID-19 reported from large-scale general hospitals in Wuhan, we hold that the treatment plan was adjusted appropriately and an individualized remedy was implemented. The recommendations discussed here were developed mainly based on the experience from Wuhan. We advise that the management strategy for ovarian cancer patients should be adjusted in the light of the local epidemic situation and formulated according to the pathological type, tumor stage and the current treatment phase. Online medical service is an effective and convenient communication platform during the pandemic.
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http://dx.doi.org/10.1186/s13048-021-00784-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891806PMC
February 2021

Effect of HMGB1 on monocyte immune function in respiratory syncytial virus bronchiolitis.

Exp Ther Med 2021 Jan 25;21(1):75. Epub 2020 Nov 25.

Department of Critical Care Medicine, Xuzhou Children's Hospital, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China.

Expression of high mobility group protein box 1 (HMGB1) in children with respiratory syncytial virus bronchiolitis and its effect on the inflammatory function of monocytes were investigated. A total of 30 cases of respiratory syncytial viral bronchitis and 30 cases of healthy persons from physical examination were collected from January 2017 to September 2019 in the pediatric department of Xuzhou Children's Hospital, Xuzhou Medical University. HMGB1 expression level in plasma was detected by ELISA. All participants in the study were followed up for 18 months. Human recombinant respiratory syncytial virus (RSV)-A2 virus was used to infect human bronchial epithelial cell line 16HBE, and cell culture supernatant was collected to detect HMGB1. Transwell plate was used to co-culture infected or no-infection groups of epithelial cells and monocytes THP-1. Western blot was used to detect the level of Toll-like receptor (TLR)4 and TLR7 in monocytes. HMGB1 expression level in peripheral blood of children with bronchiolitis was significantly increased compared with that in healthy controls (P<0.0001), and was significantly correlated with the severity of the children's condition (P<0.01). The expression level of HMGB1 was significantly correlated with the number of monocytes, lymphocytes and CRP expression level. HMGB1 was also significantly increased in cell culture supernatant compared with no-infection group (P<0.0001). TLR4 expression in monocytes could be activated by the virus infected cell lines. Follow-up results showed that children with bronchiolitis had a higher incidence of asthma within 18 months (P<0.05). The independent risk factors for children to develop asthma were age, number of monocytes and HMGB1 level. HMGB1 is highly expressed in peripheral blood of children with respiratory syncytial virus bronchitis, and RSV epithelial cells can activate TLR4 expression in monocytes, suggesting that HMGB1 plays an important role in monocyte mediated immune inflammation. HMGB1 expression level is related to the development of asthma in children, which is of great significance for understanding the pathogenesis of bronchiolitis and suggesting the prognosis of children.
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http://dx.doi.org/10.3892/etm.2020.9507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716648PMC
January 2021

Comparative Study of Auto Plan and Manual Plan for Nasopharyngeal Carcinoma Intensity-Modulated Radiation Therapy.

Cancer Manag Res 2020 2;12:12439-12445. Epub 2020 Dec 2.

Department of Radiation Therapy, Sichuan Cancer Hospital, Radiation Oncology Key Laboratory of Sichuan Province, Chengdu, People's Republic of China.

Purpose And Objective: Auto planning might reduce the manual time required for the optimization and could also potentially improve the overall plan quality. The aim of this study is to demonstrate the statistical comparison of automatic (AU) and manually (MA) generated nasopharyngeal carcinoma (NPC) intensity-modulated radiation therapy (IMRT) plans.

Materials And Methods: The study included 105 nasopharyngeal carcinoma patients, admitted to our hospital. The patients underwent IMRT treatments. The clinically delivered plans were performed with Eclipse (Version 11.0) using manual optimization. The same plans were optimized successively in Pinnacle (version 9.10) treatment planning system using the auto plan software package module. D95 (dose of 95% volume) and D98 (dose of 98% volume) were calculated for the targets and maximum dose (Dmax) and mean dose (Dmean) for the organ at risks (OARs); moreover, the average doses of each target and OARs for 105 patients were evaluated.

Results: There is no significant difference in the homogeneity of the target between AU and MA treatment plans, while a significant difference is observed for what is concerning the OARs or most of OARs in 105 patients, OAR doses were significantly reduced in AU plan. For OARs which have no significant difference between AU and MA plans are highlighted, the mean dose of OARs in AU plans was at least not higher than MA plans.

Conclusion: Nasopharyngeal carcinoma IMRT plans made by an automatic planning tool met the clinical requirements for target prescription dose; moreover, the dose of normal tissues was lower than in MA plans. Clinical physicists' time can be saved and the influence of factors such as the lack of experience in treatment planning can be avoided.
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http://dx.doi.org/10.2147/CMAR.S226495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719327PMC
December 2020

Event-Triggered Adaptive Fault Tolerant Control for a Class of Uncertain Nonlinear Systems.

Entropy (Basel) 2020 May 27;22(6). Epub 2020 May 27.

Key Laboratory of Measurement and Control of CSE Ministry of Education, School of Automation, Southeast University, Nanjing 210096, China.

This paper considers an adaptive fault-tolerant control problem for a class of uncertain strict feedback nonlinear systems, in which the actuator has an unknown drift fault and the loss of effectiveness fault. Based on the event-triggered theory, the adaptive backstepping technique, and Lyapunov theory, a novel fault-tolerant control strategy is presented. It is shown that an appropriate comprise between the control performance and the sensor data real-time transmission consumption is made, and the fault-tolerant tracking control problem of the strict feedback nonlinear system with uncertain and unknown control direction is solved. The adaptive backstepping method is introduced to compensate the actuator faults. Moreover, a new adjustable event-triggered rule is designed to determine the sampling state instants. The overall control strategy guarantees that the output signal tracks the reference signal, and all the signals of the closed-loop systems are convergent. Finally, the fan speed control system is constructed to demonstrate the validity of the proposed strategy and the application of the general systems.
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http://dx.doi.org/10.3390/e22060598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7517132PMC
May 2020

Enhancing anti-melanoma outcomes in mice using novel chitooligosaccharide nanoparticles loaded with therapeutic survivin-targeted siRNA.

Eur J Pharm Sci 2021 Mar 18;158:105641. Epub 2020 Nov 18.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address:

Melanoma anti-tumor therapy remains a challenge. SiRNA-based therapies provide a powerful means, but limitations remain in its pharmaceutical applications owing to the lack of highly efficient delivery systems. In this study, to improve the siRNA delivery efficiency of chitooligosaccharide (COS), phenylboronic acid (PBA)-modified COS was synthesized and structurally characterized. PBA-modified COS was used to deliver survivin-targeted siRNA for melanoma treatment. The siRNA-loaded nanoparticles were prepared by a synergetic assembly of electrostatic complexation and chemical cross-linking. The particle size and zeta potential were characterized by dynamic light scattering, and transmission electron microscopy was utilized to observe the morphology of the nanoparticles. The cellular uptake of nanoparticles on B16F10 cells was studied by flow cytometry and confocal laser scanning microscopy. A luciferase reporter gene assay determined the gene silencing efficiency of different nanoparticles. As a result, the novel nanoparticles remarkably inhibited the proliferation of B16F10 cells in vitro and significantly inhibited the growth and metastasis of melanoma in vivo. In conclusion, PBA-modified COS can serve as a promising carrier for siRNA delivery in the field of anti-tumor therapy.
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http://dx.doi.org/10.1016/j.ejps.2020.105641DOI Listing
March 2021

Impact of Positioning Errors on the Dosimetry of Breath-Hold-Based Volumetric Arc Modulated and Tangential Field-in-Field Left-Sided Breast Treatments.

Front Oncol 2020 29;10:554131. Epub 2020 Oct 29.

Department of Radiation Oncology, Sichuan Cancer Hospital and Research Institute, Chengdu, China.

Heart diseases and cardiovascular events are well-known side effects in left-sided breast irradiation. Deep inspiration breath hold (BH) combined with fast delivery techniques such as volumetric modulated arc therapy (VMAT) or tangential field-in-field (TFiF) can serve as a valuable solution to reduce the dose to the heart. This study aims to compare the impact of positioning errors in VMAT and TFiF plans for BH left-sided breast treatments. Fifteen left-sided breast patients treated in BH with TFiF technique were included in this retrospective study. For each patient, a second plan with VMAT technique was optimized. Eighteen setup variations were introduced in each of these VMAT and TFiF reference plans, shifting the isocenter along six different directions by 3, 5, and 10 mm. A total of 540 perturbed plans, 270 for each technique, were recalculated and analyzed. The dose distributions on the target and organs at risk obtained in the different perturbed scenarios were compared with the reference scenarios, using as dosimetric endpoints the dose-volume histograms (DVH). The results were compared using the Wilcoxon test. Comparable plan quality was obtained for the reference VMAT and TFiF plans, except for low doses to organs at risk for which higher values (p < 0.05) were obtained for VMAT plans. For TFiF plans, perturbations of the isocenter position of 3, 5, or 10 mm produced mean deviations of the target DVH dosimetric parameters up to -0.5, -1.0, and -5.2%, respectively; VMAT plans were more sensitive to positioning errors resulting in mean deviations up to -0.5, -4.9, and -13.9%, respectively, for the same magnitude of the above mentioned perturbations. For organs at risk, only perturbations along the left, posterior, and inferior directions resulted in dose increase with a maximum deviation of +2% in the DVH dosimetric parameters. A notable exception were low doses to the left lung and heart for 10 mm isocenter shifts for which the mean differences ranged between +2.7 and +4.1%. Objective information on how external stresses affect the dosimetry of the treatment is the first step towards personalized radiotherapy.
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http://dx.doi.org/10.3389/fonc.2020.554131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658584PMC
October 2020

Comparison of visible light driven HO and peroxymonosulfate degradation of norfloxacin using Co/g-CN.

Chemosphere 2021 Jan 19;262:127955. Epub 2020 Aug 19.

College of Environmental Science and Engineering, Beijing Forestry University, Beijing, 100083, PR China. Electronic address:

As common advanced oxidation processes, Fenton-like and peroxymonosulfate (PMS) processes have received enormous attention due to their high efficiency in the pollutants degradation. In this study, the Co/g-CN photocatalyst was prepared by facial calcination strategy and used to evaluate the behavior of the Co/g-CN/HO and Co/g-CN/PMS systems for norfloxacin (NOR) photocatalytic degradation under visible light irradiation. The composite photocatalysts exhibited better performance compared to that of pure g-CN due to the efficient separation of electron-hole pairs and visible light absorption. The Co/g-CN/PMS system possessed better photocatalytic performance than the Co/g-CN/HO system, where the degradation ratio of NOR and removal ratio of total organic carbon (TOC) were 96.4% and 54%, respectively, in 10 min. The photocatalytic mechanism was investigated using reactive species trapping experiments and electron spin-resonance spectroscopy (ESR). ⋅OH and SO were the dominant reaction species in the Co/g-CN/HO and Co/g-CN/PMS systems, respectively. According to the analysis of the NOR degradation path, SO could attack the C-H bond on the piperazine ring or quinolone group of NOR, which resulted in it more active and accelerating the destruction of NOR with SO and ⋅OH. The destruction of the quinolone group was the main pathway in the HO process, while the destruction of the piperazine ring was the main pathway in the PMS process. In sum, the Co/g-CN/PMS process had a higher photocatalytic activity and economic applicability.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127955DOI Listing
January 2021

[Analysis of potential pharmacodynamic material basis of Euonymus alatus in treatment of nephritis based on integrated chemomics and network biology].

Zhongguo Zhong Yao Za Zhi 2020 Sep;45(18):4429-4439

School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China Hubei University of Medicine, Institute of Wudang Traditional Chinese Medicine, Taihe Hospital Shiyan 442000, China.

This paper was aimed to explore the potential pharmacodynamics effect of Euonymus alatus in the treatment of nephritis based on integrated chemomics and network biology. The chemical constituent database of E. alatus was constructed by consulting litera-ture and using online database. The chemical constituents were identified by UPLC-Q-TOF/HRMS~E and UNIFI software. On this basis, a series of comparisons, molecular docking studies and in-depth analysis of the chemical constituents and nephritis disease targets were carried out with use of network biology method, and the potential pharmacodynamic effect of E. alatus for the treatment of nephritis was investigated by reviewing the existing. In this study, 62 chemical constituents were collected in the database of chemical consti-tuents of E. alatus, and 24 chemical constituents were identified by mass spectrum. Subsequently, based on the network biology me-thod, 22 important chemical constituents and 5 key targets were obtained by reverse screening. Molecular docking study showed that a total of 11 chemical constituents such as quercetin, kaempferol, and catechinmay be the potential material basis for E. alatus in the treatment of nephritis. Starting with chemomics and using the technology of network biology, we established a network interaction model between drug components and disease targets in this study. Through the interaction between targets in complex networks, we can find the key targets easily and quickly. By docking the key targets with small drug molecules, we can screen out the potential pharmacodynamic components, providing a reference for the follow-up study of active ingredients.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200520.402DOI Listing
September 2020

Responses of Nitrogen and Phosphorus Removal Performance and Microbial Community to FeO@SiO Nanoparticles in a Sequencing Batch Reactor.

Appl Biochem Biotechnol 2021 Feb 10;193(2):544-559. Epub 2020 Oct 10.

College of Resources and Environment, Chengdu University of Information Technology, Chengdu, 610225, China.

The responses of total nitrogen (TN) and total phosphorus (TP) removal performance and microbial community to 0-1.2 g/L FeO@SiO nanoparticles (NPs) in sequencing batch reactors were investigated. Results showed that an appropriate dose of FeO@SiO NPs (0.3 g/L) could promote the removal efficiency of TN and TP. High-throughput sequencing results indicated that microbial richness increased, whereas microbial diversity did not vary upon exposure to 0.1-1.2 g/L FeO@SiO NPs. The relative abundances of Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria increased from 11.75%, 3.52%, and 6.77%, respectively, at 0 g/L FeO@SiO to 27.05%, 7.21%, and 14.77%, respectively, upon exposure to 0.3 g/L FeO@SiO. At the genus level, 0.3 g/L FeO@SiO NPs enriched norank_f_Nitrosomonadaceae, norank_f_Xanthomonadaceae, Amaricoccus, and Shinella. Real-time quantitative polymerase chain reaction results suggested that the gene copy number of ammonium-oxidizing, nitrite-oxidizing, and denitrifying bacteria population remarkably increased, whereas the number of phosphorus-accumulating organisms slightly increased under long-term exposure to 0.3 g/L FeO@SiO NPs. Energy-dispersive spectrum analysis showed that the phosphorus content was higher at 0.3 g/L FeO@SiO than at 0 g/L FeO@SiO. Nitrogen removal primarily occurred through a biological mechanism, while most phosphorus in wastewater may be removed by the combination of physicochemical and biological methods.
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http://dx.doi.org/10.1007/s12010-020-03441-4DOI Listing
February 2021

Liraglutide modulates gut microbiome and attenuates nonalcoholic fatty liver in db/db mice.

Life Sci 2020 Nov 19;261:118457. Epub 2020 Sep 19.

Shuguang Hospital Affliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203,China. Electronic address:

Aims: Liraglutide, a glucagon-like peptide-1(GLP-1) analog, is effective for the treatment of type II diabetes and nonalcoholic fatty liver disease (NAFLD). It was proved that gut microbiome plays a role in the development of NAFLD. This study aims to observe the therapeutic effect of liraglutide on nonalcoholic fatty liver (NAFL) in mice and effect on the gut microbial community.

Main Methods: The db/db mice were used as the NAFL model, and lactulose was used as the positive control drug. Hepatic triglyceride, liver histopathology, and indices of glucolipid metabolism, including fasting blood glucose, fasting insulin, insulin resistance index and blood lipids were evaluated after treatment of liraglutide or lactulose for four weeks. The colonic microbiome of the mice was analyzed by 16S rRNA gene sequencing.

Key Findings: Liraglutide significantly reduced the hepatic triglyceride (TG) content, alanine aminotransferase (ALT) activity, fasting blood glucose, insulin resistance and serum low density lipoprotein (LDL) in the db/db mice. In terms of hepatic pathologies, hepatic steatosis was significantly improved after liraglutide treating. Microbiome analysis revealed that liraglutide significantly increased the abundance of Akkermansia, Romboutsia, norank_f_Bacteroidales_S24-7_group, and decreased the abundance of Klebsiella, Anaerotruncus, Bacteroides, Lachnospiraceae_UCG-001, Lachnospiraceae_NK4A136_group, Ruminiclostridium, uncultured_f__Ruminococcaceae, and Desulfovibrio.

Significance: The results of the present study suggested that liraglutide had a certain therapeutic effect on fatty liver in db/db mice and had an impact on the composition of the intestinal microflora, especially some bacteria related to glucolipid metabolism and intestinal inflammation. Affecting gut microbiome might be a potential mechanism of liraglutide in treating NAFL.
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http://dx.doi.org/10.1016/j.lfs.2020.118457DOI Listing
November 2020

[Progress in clinical application of 3D printed navigational template in orthopedic surgery].

Nan Fang Yi Ke Da Xue Xue Bao 2020 Aug;40(8):1220-1224

Department of Human Anatomy, School of Basic Medical Science, Southern Medical University//Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangzhou 510515, China.

Orthopedic 3D printed surgical navigational template is an instrument that is prepared by 3D reconstruction based on preoperative radiological data of the patient using computer-aided design (CAD) and 3D printing techniques. The 3D printed navigational template allows accurate intra-operative assessment of the relative spatial distance, angular relationship, direction and depth. The application of 3D printed navigational template technique in orthopedics surgeries achieves the conversion of preoperative planning from 2/3D graphics to 3D models, and provides a new method for individualized and precise treatment. Herein we review the evolution, clinical application, and basic classification of 3D printed navigation template technique, analyze its advantages and disadvantages, and discuss the current problems and the future development of this technique.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2020.08.22DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429156PMC
August 2020

Plastic optical fiber sensor for temperature-independent high-sensitivity detection of humidity.

Appl Opt 2020 Jul;59(19):5708-5713

A simple U-shaped plastic optical fiber evanescent-wave sensor was fabricated for temperature-independent highly sensitive detection of humidity. The sensing region of the sensor was subjected to five cycles of heating-cooling to improve temperature independence. The effects of the polyimide (PI) coating thickness, number of graphene oxide (GO) coating layers, and alternate PI-GO coating sequence were investigated to optimize sensitivity. The fabricated sensor exhibited high-temperature independence and good sensitivity of 0.17×10 (% relative ) in the temperature range of 10°C to 70°C.
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http://dx.doi.org/10.1364/AO.391090DOI Listing
July 2020

[Bacterial Community Shifts and Nitrogen Removal Characteristics for a SNAD Process Treating Anaerobic Digester Liquor of Swine Wastewater (ADLSW) in a Continuous-Flow Biofilm Reactor (CFBR)].

Huan Jing Ke Xue 2020 May;41(5):2349-2357

College of Resources and Environment, Chengdu University of Information Technology, Chengdu 610225, China.

To realize a simultaneous partial nitrification, ANAMMOX (anaerobic ammonium oxidation), and denitrification (SNAD) process treating anaerobic digester liquor of swine wastewater (ADLSW) in a continuous-flow biofilm reactor (CFBR), we first gradually increased the influent ammonium (NH-N) concentration, and then enhanced the ADLSW ratio in the influent during operation; dissolved oxygen (DO) was controlled at (0.4±0.1) mg·L by adjusting the air flow rate, and the temperature was kept at (30±1)℃. Meanwhile, high-throughput sequencing and quantitative PCR (polymerase chain reaction) techniques were used to analyze the bacterial community shifts and the amount of dominant nitrogen removal bacteria. The results demonstrated that a successful start-up of the SNAD process was accomplished in 150 d, and replacement of the actual biogas slurry was completed in 298 d. The effluent (NO-N+NO-N)/ΔNH-N value was less than 0.11, and the average removal rates of NH-N and TN (total nitrogen) increased to 63.26% and 55.71%, respectively. Moreover, high-throughput sequencing results demonstrated that the dominant microbial populations at phylum level were Chloroflexi (with a relative abundance of 50.78%), Proteobacteria (13.34%), and Planctomycetes (9.26%). The relative abundance of increased from 1.55% to 1.98%. In addition, the relative abundance of and increased from 0.01% and (<0.01%) to 4.66% and 4.18%, respectively, and the relative abundance of increased from (<0.01%) to 2.06%. Meanwhile, qPCR analysis showed that the amounts of ammonia-oxidizing bacteria, ANAMMOX, and denitrifying bacteria increased significantly compared with the inoculated sludge. An efficient and stable nitrogen removal rate can be achieved, and the follow-up processing cost can be reduced, by application of the SNAD treatment process for ADLSW.
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http://dx.doi.org/10.13227/j.hjkx.201910018DOI Listing
May 2020

High-trans fatty acid and high-sugar diets can cause mice with non-alcoholic steatohepatitis with liver fibrosis and potential pathogenesis.

Nutr Metab (Lond) 2020 26;17:40. Epub 2020 May 26.

Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Road, Pudong New Area, Shanghai, 201203 China.

Background And Aims: Even Non-alcoholic steatohepatitis (NASH) has been becoming the key role in process of liver fibrosis or cirrhosis, no any NASH involving liver fibrosis mice model which consistent with the mechanisms of fatty acid and glucose metabolism disorder was widely accepted. Here, we established a mouse model of nonalcoholic steatohepatitis (NASH) with liver fibrosis using a high-fat, high-carbohydrate diet (HFHC) and analyzed the potential pathogenesis using a transcriptome microarray.

Methods: Fifty mice were stratified by weight and randomly divided into the HFHC model and control (Con) groups. Ten mice were sacrificed at the beginning of the experiments, 10 mice of HFHC and Con group were euthanized at the end of 20 and 30 weeks. The following analyses were performed: biochemical analysis; histological assessment; evaluation of hepatic type I collagen (Col-I), α-smooth muscle actin (α-SMA) and transforming growth factor-β1 (TGF-β1) protein and mRNA expression levels; and transcriptomic gene chip analysis.

Results: Compared with the Con group at each time point, the body weight and liver wet weight of the HFHC model group of mice were significantly higher. At 30th weeks, alanine aminotransferase (ALT), aspartate aminotransferase (AST), fasting blood glucose (FBG) and fasting insulin (FINS) levels or activities and the triglyceride (TG) and hydroxyproline (HYP) content in the HFHC model group were significantly elevated. Severe steatosis was present in the liver tissues contributed from the HFHC group of mice. Typically, substantial perisinusoidal fibrosis with a cage-like structure and bridging formations were observed in the mice liver in HFHC group. Col-I, α-SMA and TGF-β1 protein and mRNA expression levels in liver tissues of HFHC mice dramatically increased over time. Compared with the Con group, the HFHC group had 151 differentially expressed genes that were involved in 41 signaling pathways.

Conclusions: After keeping 30 weeks HFHC diet treatment, the mice exhibited substantial liver fibrosis, hepatic steatosis, ballooning degeneration and inflammation. Basing on the transcriptome microarray assays, the experimental NASH involving liver fibrosis potentially related to dramatically changed ECM-receptor interaction, Toll-like receptor signaling and other signaling pathways.
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http://dx.doi.org/10.1186/s12986-020-00462-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249374PMC
May 2020

Tetramethylpyrazine enhanced the therapeutic effects of human umbilical cord mesenchymal stem cells in experimental autoimmune encephalomyelitis mice through Nrf2/HO-1 signaling pathway.

Stem Cell Res Ther 2020 05 19;11(1):186. Epub 2020 May 19.

Department of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai, China.

Introduction: The therapeutic effects of mesenchymal stem cells (MSCs) have been limited by their apoptosis induced by oxidative stress after delivery into the injured sites. Therefore, strategies designed to improve the MSC therapeutic efficacy need to be explored. Tetramethylpyrazine (TMP) can promote the proliferation and differentiation of neural stem cells. In this study, we first evaluated the effects and mechanism of TMP on HO-stimulated human umbilical cord MSCs (hUCMSCs) and then further investigated the therapeutic effects of TMP-stimulated hUCMSCs on experimental autoimmune encephalomyelitis (EAE) mice.

Methods: The toxicity of hUCMSCs against of TMP was determined by cell count kit-8 (CCK-8) assay. The effects of TMP on the hUCMSC cell cycle, the reactive oxygen species (ROS) production, and the apoptosis of HO-stimulated hUCMSCs were determined by flow cytometry. The expression of malondialdehyde (MDA) and superoxide dismutase (SOD) were also measured by colorimetry. The signaling pathway of TMP induced on HO-stimulated hUCMSCs was investigated by western blot. EAE was induced using immunization with MOG35-55 in C57BL/6 mice. The inflammatory cell infiltration and demyelination were detected by immunofluorescence staining. The blood-brain barrier (BBB) disruption was detected by Evans blue (EB) stain and the expression of tight junction protein (ZO-1) by western blot.

Results: TMP significantly increased cell viability and changed the cell cycle of hUCMSCs. In addition, TMP (100 μM) significantly reduced intracellular ROS production, expression of MDA, and apoptosis, but increased expression of SOD through nuclear factor-erythroid 2-related factor-2 (Nrf2)/heme oxygenase 1 (HO-1) signaling pathway in HO-stimulated hUCMSCs. Most importantly, compared with wild hUCMSCs, TMP-stimulated hUCMSCs significantly ameliorated EAE, by attenuation of inflammation, demyelination, and BBB disruption.

Conclusion: The TMP-stimulated hUCMSCs provide a potential therapeutical protocol to enhance the therapeutic effects of hUCMSCs in multiple sclerosis.
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http://dx.doi.org/10.1186/s13287-020-01700-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238657PMC
May 2020

Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis.

Nutr Metab (Lond) 2020 1;17:34. Epub 2020 May 1.

1Institute of Liver Disease, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Gypenosides (Gyp) are the main ingredient of the Chinese medicine, They are widely used in Asia as a hepatoprotective agent. Here, we elucidated the mechanism of Gyp in non-alcoholic steatohepatitis (NASH) with a focus on farnesoid X receptor (FXR)-mediated bile acid and lipid metabolic pathways.

Methods: NASH was induced in mice by high-fat diet (HFD) feeding, while mice in the control group were given a normal diet. At the end of week 10, HFD-fed mice were randomly divided into HFD, HFD plus Gyp, and HFD plus obeticholic acid (OCA, FXR agonist) groups and were given the corresponding treatments for 4 weeks. Next, we analyzed the histopathological changes as well as the liver triglyceride (TG) level and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), fasting blood glucose (FBG), fasting insulin (FINS), TG, total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels as well as the bile acid profile. We carried out RT-PCR and western blotting to detect HFD-induced alterations in gene/protein expression related to bile acid and lipid metabolism.

Results: The HFD group had histopathological signs of hepatic steatosis and vacuolar degeneration. The liver TG and serum ALT, AST, FBG, FINS, TC, and LDL-C levels as well as the total bile acid level were significantly higher in the HFD group than in the control group ( < 0.01). In addition, we observed significant changes in the expression of proteins involved in bile acid or lipid metabolism ( < 0.05). Upon treatment with Gyp or OCA, signs of hepatic steatosis and alterations in different biochemical parameters were significantly improved ( < 0.05). Further, HFD-induced alterations in the expression genes involved in bile acid and lipid metabolism, such as CYP7A1, BSEP, SREBP1, and FASN, were significantly alleviated.

Conclusions: Gyp can improve liver lipid and bile acid metabolism in a mouse model of NASH, and these effects may be related to activation of the FXR signaling pathway.
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http://dx.doi.org/10.1186/s12986-020-00454-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195801PMC
May 2020

Astaxanthin Attenuates Hypertensive Vascular Remodeling by Protecting Vascular Smooth Muscle Cells from Oxidative Stress-Induced Mitochondrial Dysfunction.

Oxid Med Cell Longev 2020 14;2020:4629189. Epub 2020 Apr 14.

Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.

Oxidative stress aggravates mitochondrial injuries and accelerates the proliferation of vascular smooth muscle cells (VSMCs), which are important mechanisms contributing to vascular remodeling in hypertension. We put forward the hypothesis that Astaxanthin (ATX), known to possess strong features of antioxidant, could attenuate vascular remodeling by inhibiting VSMC proliferation and improving mitochondrial function. The potential effects of ATX were tested on spontaneously hypertensive rats (SHRs) and cultured VSMCs that injured by angiotensin II (Ang II). The results showed that ATX lowered blood pressure, reduced aortic wall thickness and fibrosis, and decreased the level of reactive oxygen species (ROS) and HO in tunica media. Moreover, ATX decreased the expression of proliferating cell nuclear antigen (PCNA) and ki67 in aortic VSMCs. , ATX mitigated VSMC proliferation and migration, decreased the level of cellular ROS, and balanced the activities of ROS-related enzymes including NADPH oxidase, xanthine oxidase, and superoxide dismutase (SOD). Besides, ATX mitigated Ca overload, the overproduction of mitochondrial ROS (mtROS), mitochondrial dysfunction, mitochondrial fission, and Drp1 phosphorylation at Ser616. In addition, ATX enhanced mitophagy and mitochondrial biosynthesis by increasing the expression of PINK, parkin, mtDNA, mitochondrial transcription factor A (Tfam), and PGC-1. The present study indicated that ATX could efficiently treat vascular remodeling through restraining VSMC proliferation and restoring mitochondrial function. Inhibiting mitochondrial fission by decreasing the phosphorylation of Drp1 and stimulating mitochondrial autophagy and biosynthesis via increasing the expression of PINK, parkin, Tfam, and PGC-1 may be part of its underlying mechanisms.
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http://dx.doi.org/10.1155/2020/4629189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178508PMC
February 2021

microRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat.

Diabetes Metab Syndr Obes 2020 15;13:1147-1159. Epub 2020 Apr 15.

Department of Cardiology, The Central Laboratory, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China.

Purpose: A high-fat diet (HFD) can lead to cardiac dysfunction, hypertrophy, and fibrosis. This study aimed to explore microRNA expression profiles in the myocardium of HFD-induced obesity rat.

Materials and methods: Wistar rats were randomly divided into two groups, and fed with normal chow diet (NCD) or HFD for 20 weeks. Cardiac function was evaluated by echocardiography. Left ventricular myocardium was harvested to assess the extent of myocardial morphology alteration. MicroRNA expression was analyzed using Agilent miRNA microarray and quantitative real-time PCR (qRT-PCR) was used to validate the microarray data. The mirdbV6 database was used to forecast the miRNA target genes. The role of microRNAs in palmitate-induced cardiac hypertrophy and fibrosis in primary neonatal rat cardiomyocytes was evaluated by loss- and gain-of-function experiments.

Results: Significant changes in cardiac function, hypertrophy, fibrosis, and apoptosis were found in HFD rats as compared with NCD rats. miR-141-3p and miR-144-3p were also significantly upregulated in the myocardium of HFD-induced obesity rat. A series of genes involved in essential biological processes, including anatomical structure development and metabolic process, was targeted by these two miRNAs. These target genes were also implicated in signaling pathways involved in the PI3K-Akt signaling pathway, Wnt signaling pathway, autophagy, and protein processing in the endoplasmic reticulum. Inhibition of miR-141 or overexpression of miR-144 attenuated palmitate-induced cardiac hypertrophy and fibrosis. In contrast, overexpression of miR-141 or inhibition of miR-144 aggravated palmitate-induced cardiac hypertrophy and fibrosis.

Conclusion: This study identifies that miR-141 and miR-144 are candidate miRNAs associated with the development of HFD-induced cardiac dysfunction and structure alteration.
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http://dx.doi.org/10.2147/DMSO.S248948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167270PMC
April 2020

A polystyrene-degrading Acinetobacter bacterium isolated from the larvae of Tribolium castaneum.

Sci Total Environ 2020 Jul 10;726:138564. Epub 2020 Apr 10.

Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

Polystyrene (PS) has been widely used in various fields, whereas this thermoplastic material is generally considered to be resistant to biodegradation. Tribolium castaneum (Coleoptera: Tenebrionidae), a common pest of stored agricultural products, is a powerful model organism for general insect research. In this study, the larvae of T. castaneum were observed chewing and eating extruded polystyrene foam (XPS). Investigation of the gut microbiome of plastic- and bran-fed T. castaneum larvae showed that Acinetobacter sp. was strongly associated with PS ingestion. Additionally, one bacterial strain capable of PS degradation, was successfully isolated from the gut of these larvae and identified as Acinetobacter sp. AnTc-1 by its 16S rDNA sequence. Gel penetration chromatography (GPC), H nuclear magnetic resonance (H NMR) spectroscopy, thermo gravimetric analysis (TGA) and scanning electron microscope (SEM) were employed to characterize the PS degradation. After incubation with AnTc-1 for 60 days, the mass weight (12.14%) and molecular weight (13%/25%, weight-average molecular weight (Mw)/number-average molecular weight (Mn)) of PS powder were significantly reduced. The results indicated that the isolated strain of Acinetobacter sp. AnTc-1 has PS-degrading capacity. The isolated strain may play a role in the larval gut for biodegradation of PS and has potential to be applied for petroleum-based plastic degradation study and development of remediation approaches.
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http://dx.doi.org/10.1016/j.scitotenv.2020.138564DOI Listing
July 2020

Metabolic Engineering of Histidine Kinases in for Enhanced Butanol Production.

Front Bioeng Biotechnol 2020 20;8:214. Epub 2020 Mar 20.

School of Bioengineering, Dalian University of Technology, Dalian, China.

, a promising industrial microorganism for butanol production, suffers from low butanol titer and lack of high-efficiency genetical engineering toolkit. A few histidine kinases (HKs) responsible for Spo0A phosphorylation have been demonstrated as functionally important components in regulating butanol biosynthesis in solventogenic clostridia such as , but no study about HKs has been conducted in . In this study, six annotated but uncharacterized candidate HK genes sharing partial homologies (no less than 30%) with those in were selected based on sequence alignment. The encoding region of these HK genes were deleted with CRISPR-Cas9n-based genome editing technology. The deletion of and resulted in significant change in butanol biosynthesis, with butanol production increased by 40.8 and 17.3% (13.8 g/L and 11.5 g/L vs. 9.8 g/L), respectively, compared to the wild-type. Faster butanol production rates were observed, with butanol productivity greatly increased by 40.0 and 20.0%, respectively, indicating these two HKs are important in regulating cellular metabolism in . In addition, the sporulation frequencies of two HKs inactivated strains decreased by 96.9 and 77.4%, respectively. The other four HK-deletion (including , and ) mutant strains showed few phenotypic changes compared with the wild-type. This study demonstrated the role of HKs on sporulation and solventogenesis in , and provided a novel engineering strategy of HKs for improving metabolite production. The hyper-butanol-producing strains generated in this study have great potentials in industrial biobutanol production.
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http://dx.doi.org/10.3389/fbioe.2020.00214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098912PMC
March 2020

Meta-analysis on the use of hyaluronic acid gel to prevent intrauterine adhesion after intrauterine operations.

Exp Ther Med 2020 Apr 3;19(4):2672-2678. Epub 2020 Feb 3.

Department of Human Health and Human Services, Yiwu Maternity and Children Hospital, Jinhua, Zhejiang 322000, P.R. China.

Intrauterine adhesion (IUA) is a severe complication that occurs following abortion, and hyaluronic acid gel has been used to prevent IUA following intrauterine operation. The present study aimed to systematically evaluate the efficacy of hyaluronic acid gel in preventing IUA following intrauterine operation. In the current study, a literature search was performed using PubMed, Cochrane Library and EMbase databases for randomized controlled clinical trials that used hyaluronic acid gel as an adjuvant therapy following intrauterine operation. The terms 'hyaluronic acid', 'intrauterine adhesions', 'Asherman's syndrome', 'IUA', 'dilatation and curettage', 'abortion' and 'hysteroscopic' were used to search for articles published online before July 31, 2018. RevMan 5.3 software was used to analyze the indicators of uterine cavity adhesion formation and pregnancy rates following intrauterine operation. A total of seven randomized controlled clinical studies were included, consisting of 952 patients who underwent intrauterine operation. The meta-analysis indicated that the use of hyaluronic acid gel reduced the incidence of IUA [relative risk (RR)=0.42; 95% confidence interval (CI)=0.30-0.57; P<0.001] and the score for IUA after an intrauterine operation (mean difference=-1.29; 95%=-1.73 to -0.84; P<0.001). A subgroup analysis revealed that the preventive effect of hyaluronic acid gel on IUA was not affected by the type of intrauterine operation, namely abortion (RR=0.40; 95% CI=0.26-0.62; P<0.001) and hysteroscopy (RR=0.44; 95% CI=0.28-0.68; P<0.001). The preventive effect of hyaluronic acid gel on IUA was also not affected by primary disorders/diseases including the following: Abortion (RR=0.48; 95% CI=0.29-0.78; P=0.003); IUA (RR=0.38; 95% CI=0.21-0.67; P<0.001) and submucosal myoma of the uterus, endometrial polyps or mediastinum uterus (RR=0.40; 95% CI=0.18-0.90; P=0.03). Hyaluronic acid gel improved pregnancy rates after intrauterine operations (RR=1.94; 95% CI=1.46-2.60; P<0.001). In conclusion, hyaluronic acid gel was indicated to significantly reduce the incidence of IUA following intrauterine operation, regardless of the type of intrauterine operation or the presence of primary diseases. Treatment with hyaluronic acid gel was also revealed to increase pregnancy rates following intrauterine operation.
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http://dx.doi.org/10.3892/etm.2020.8483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086218PMC
April 2020

Hybrid Solvation Model with First Solvation Shell for Calculation of Solvation Free Energy.

Chemphyschem 2020 04 10;21(8):762-769. Epub 2020 Mar 10.

College of Physics and Electronics, Shandong Normal University, Jinan, Shandong, 250014, China.

We present a hybrid solvation model with first solvation shell to calculate solvation free energies. This hybrid model combines the quantum mechanics and molecular mechanics methods with the analytical expression based on the Born solvation model to calculate solvation free energies. Based on calculated free energies of solvation and reaction profiles in gas phase, we set up a unified scheme to predict reaction profiles in solution. The predicted solvation free energies and reaction barriers are compared with experimental results for twenty bimolecular nucleophilic substitution reactions. These comparisons show that our hybrid solvation model can predict reliable solvation free energies and reaction barriers for chemical reactions of small molecules in aqueous solution.
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http://dx.doi.org/10.1002/cphc.202000039DOI Listing
April 2020

Salidroside improves high-fat diet-induced non-alcoholic steatohepatitis by regulating the gut microbiota-bile acid-farnesoid X receptor axis.

Biomed Pharmacother 2020 Apr 25;124:109915. Epub 2020 Jan 25.

Institute of Liver Disease, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. Electronic address:

Background: Our previous studies found that salidroside can effectively treat non-alcoholic steatohepatitis (NASH). Here, we discuss the mechanism of salidroside in the treatment of NASH with a focus on the gut microbiota-bile acid-farnesoid X receptor axis.

Methods: A NASH mouse model was created by providing mice with a high-fat diet (HFD) for 14 weeks. Mice were randomly divided into the HFD group, HFD + salidroside treatment group, and HFD + obeticholic acid treatment group (n = 8 in each group) and were intragastrically administered corresponding drugs for 4 weeks. Hematoxylin-eosin staining was performed to evaluate the histopathological changes associated with the various treatments. In addition, liver triglyceride (TG) content, serum alanine aminotransferase (ALT) activity, serum inflammatory factors, gut microbiota diversity, and the bile acid profile were evaluated. Western blotting and RT-PCR were performed to detect the expressions of FXR and fibroblast growth factor 15 (FGF15).

Results: The HFD group displayed obvious signs of hepatic steatosis. The liver TG, serum ALT, and IL-1a, IL-12, MCP-1, KC, MIP-1a, and MIP-1β were significantly higher in the HFD group than the control group (P < 0.01). Intestinal bacteria and bile acid profiles changed significantly in the HFD group (P < 0.05). Further, the expressions of FXR and FGF15 decreased significantly in the HFD group (P < 0.05). After treatment with salidroside, liver steatosis, TG content, and serum inflammatory factors significantly improved and HFD-induced intestinal bacteria, bile acid disorder, and FXR deficiency were significantly alleviated (P < 0.05).

Conclusion: Salidroside can improve NASH via the gut microbiota-bile acid-FXR axis.
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http://dx.doi.org/10.1016/j.biopha.2020.109915DOI Listing
April 2020

PHLDA1 is a new therapeutic target of oxidative stress and ischemia reperfusion-induced myocardial injury.

Life Sci 2020 Mar 22;245:117347. Epub 2020 Jan 22.

Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China. Electronic address:

Aim: Oxidative stress plays an important role in myocardial ischemia-reperfusion injury. Pleckstrin homology-like domain, family A, member 1 (PHLDA1) was first identified in apoptosis induced by T cell receptor activation, and was shown to play a different role in different cell types and under different stimuli. The role and mechanism of PHLDA1 in oxidative stress-induced cardiomyocyte injury and cardiac ischemia-reperfusion were therefore determined.

Main Methods: Cell viability and apoptotic rate were measured by Cell Counting Kit-8 and flow cytometry, respectively. Mitochondrial membrane potential was measured using JC-1 test kit. Reactive oxygen species (ROS) production was detected using ROS kit. HE staining was used to detect histological morphology, 2,3,5-triphenyltetrazolium chloride staining to detect infarct size, terminal deoxynucleotidyl transferase dUTP nick end labeling staining to detect the apoptotic rate, and immunohistochemistry and western blot analysis to detect protein expression. The binding of PHLDA1 to Bcl-2 associated X (Bax) was detected by immunoprecipitation.

Key Findings: The results indicated that PHLDA1 is highly expressed in oxidative stress-induced cardiomyocyte and myocardial ischemia-reperfusion injuries. PHLDA1 overexpression in cardiomyocytes promoted oxidative stress-induced cardiomyocyte injury. At the same time, PHLDA1 knockdown improved oxidative stress-induced cardiomyocyte and myocardial ischemia-reperfusion injuries. In addition, PHLDA1 binds to Bax and the interaction is enhanced under HO stimulation.

Significance: The present results indicated that PHLDA1 interacts with Bax to participate in oxidative stress-induced cardiomyocyte injury and myocardial ischemia reperfusion injury.
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http://dx.doi.org/10.1016/j.lfs.2020.117347DOI Listing
March 2020

The clinical efficacy study of treatment to Chiari malformation type I with syringomyelia under the minimally invasive surgery of resection of Submeningeal cerebellar Tonsillar Herniation and reconstruction of Cisterna magna.

Saudi J Biol Sci 2019 Dec 25;26(8):1927-1931. Epub 2019 Jul 25.

Department of Neurospinal Surgery, Yuquan Hospital of Tsinghua University, Beijing 10000, China.

Purpose: Discuss the clinical efficacy of treatment to Chiari malformation type I with syringomyelia under the minimally invasive surgery of resection of Submeningeal Cerebellar Tonsillar Herniation and reconstruction of Cisterna magna.

Methods: 130 Chiari malformation type I with syringomyelia patients, divided into treatment group, literature group and control group, were collected to be treated under the monitoring of ultrasound in the surgery.

Results: 6 months after operation, the lesions were decreased or disappeared, the symptoms were relieved obviously. According to MRI and Mimics 17.0 software, the volumes of Cisterna magna increased distinctly ( < 0.001), the proportions of brain in foramen magnum region were decreased ( < 0.001). Assessed by CCOS scale and Tator methods, the improvement rates of treatment group were 97.7% and 94.6%, the literature group and control group were 82.2% and 77.8%, respectively.

Conclusion: The efficacy of Chiari malformation type I with syringomyelia under the minimally invasive surgery of resection of Submeningeal Cerebellar Tonsillar Herniation and reconstruction of Cisterna magna is remarkable, and the complications are fewer. This surgery emphasizes recovery of tonsil of cerebellum and reconstruction of Cisterna magna and the circulation path of cerebrospinal fluid, which is a safe and efficient treatment.
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http://dx.doi.org/10.1016/j.sjbs.2019.07.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921303PMC
December 2019

Meta-analysis on the use of hyaluronic acid gel to prevent recurrence of intrauterine adhesion after hysteroscopic adhesiolysis.

Taiwan J Obstet Gynecol 2019 Nov;58(6):731-736

Department of Human Health and Human Services, Yiwu Maternity and Children Hospital, Jinhua, China. Electronic address:

Intrauterine adhesion is a severe complication after intrauterine operation, Various adjuvant therapies failed to improve clinical symptoms and pregnancy rates among patients with moderate-to-severe intrauterine adhesion. At present, hyaluronic acid gel is widely used in the primary prevention of adhesion after hysteroscopic adhesiolysis. However, its efficacy is still under debate. Therefore, the aim of this study was to systematically evaluate the efficacy of hyaluronic acid gel in preventing the recurrence of intrauterine adhesion after hysteroscopic adhesiolysis. The Cochrane Library, Embase, and PubMed databases were used to search for articles published before July 31, 2018, using the following terms: hyaluronic acid, intrauterine adhesions, Asherman's syndrome, IUA, hysteroscopy, and hysteroscopic adhesiolysis. Studies on therapies after hysteroscopic adhesiolysis were collected. The recurrence rate of and pregnancy rate in the presence of intrauterine adhesion after hysteroscopic adhesiolysis were analyzed by RevMan 5.3 software. A total of 6 articles were selected, which included 394 patients who were subjected to hysteroscopic adhesiolysis. The meta-analysis results showed that (1) no statistically significant difference was found between hyaluronic acid gel use and without its use on the score of intrauterine adhesion after hysteroscopic adhesiolysis [the mean difference (MD) = -0.89, 95% confidence interval (CI) (-2.53-0.76), P = 0.29], neither a statistically significant difference was observed between the same groups on the recurrence rate of intrauterine adhesion [odds ratio (OR) = 0.75, 95% CI (0.31-1.81), P = 0.53]; (2) subgroup analysis showed that hyaluronic acid gel could reduce the rate of intrauterine adhesion recurrence in randomized controlled trials [OR = -0.28, 95% CI (0.14-0.56), P = 0.0006]. However, the recurrence rate of intrauterine adhesion after the use of hyaluronic acid gel was not statistically significant in non-randomized controlled experiments [OR = 1.53, 95% CI (0.79-2.95), P = 0.21]; (3) hyaluronic acid gel did not result in a significant effect on pregnancy rate after intrauterine adhesion separation [OR = 2.02, 95% CI (0.53-7.66), P = 0.3]. In conclusion, hyaluronic acid gel could reduce the recurrence rate of intrauterine adhesion, but had no significant effect on the postoperative pregnancy rate.
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http://dx.doi.org/10.1016/j.tjog.2019.09.002DOI Listing
November 2019

Meta-analysis of the use of hyaluronic acid gel to prevent intrauterine adhesions after miscarriage.

Eur J Obstet Gynecol Reprod Biol 2020 Jan 22;244:1-4. Epub 2019 Oct 22.

Department of Human Health and Human Services, Yiwu Maternity and Children's Hospital, Jinhua, China. Electronic address:

Background: Intrauterine adhesions are a severe complication after miscarriage. Hyaluronic acid gel has been used to prevent intrauterine adhesions after miscarriage.

Objective: To systematically evaluate the efficacy of adjuvant therapy with hyaluronic acid gel to prevent intrauterine adhesions after miscarriage.

Search Strategy: The Cochrane Library, Embase and PubMed databases were searched for articles published before 31 July 2018 using the terms: ('hyaluronic acid gel' or 'gel') and ('dilatation and curettage' or 'D&C' or 'abortion' or 'miscarriage' or 'hysteroscopic') and ('intrauterine adhesions' or 'Asherman syndrome' or 'IUA' or 'endometrial injury' or 'intrauterine adhesion').

Selection Criteria: Randomized controlled trials of hyaluronic acid gel therapy after miscarriage.

Data Collection And Analysis: Four studies were included in the meta-analysis (625 patients in total). Dichotomous outcomes were expressed as relative risk (RR) with 95 % confidence intervals (CI). Continuous variables were expressed as standardized mean differences (SMD).

Main Results: Hyaluronic acid gel reduced the intrauterine adhesion scores after miscarriage (SMD -0.68, 95 % CI -1.08~-0.28; p = 0.0008) and the incidence of postoperative intrauterine adhesions after miscarriage (RR 0.44, 95 % CI 0.29~0.67; p = 0.0001). Subgroup analysis found that hyaluronic acid gel reduced the incidence of moderate and severe intrauterine adhesions after miscarriage (RR 0.18, 95 % CI: 0.07~0.47; p = 0.0004), but had no effect on the incidence of mild intrauterine adhesions (RR 0.77, 95 % CI 0.42~1.19; p = 0.19). Hyaluronic acid gel also improved the pregnancy rate after miscarriage (RR 1.94, 95 % CI 1.46~2.60; p < 0.00001).

Conclusion: Hyaluronic acid gel significantly reduced the incidence of moderate and severe intrauterine adhesions and significantly improved the pregnancy rate after miscarriage.
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http://dx.doi.org/10.1016/j.ejogrb.2019.10.018DOI Listing
January 2020