Publications by authors named "Xin Song"

521 Publications

Knockdown CYP2S1 inhibits lung cancer cells proliferation and migration.

Cancer Biomark 2021 Jul 9. Epub 2021 Jul 9.

Department of Oncology of Yan'an Hospital Affiliated to Kunming Medical University, Kunming, Yunnan, China.

Background: The incidence of lung cancer in Yunnan area ranks firstly in the world and underlying molecular mechanisms of lung cancer in Yunnan region are still unclear. We screened a novel potential oncogene CYP2S1 used mRNA microassay and bioinformation database. The function of CYP2S1 in lung cancer has not been reported.

Objective: To investigate the functions of CYP2S1 in lung cancer.

Methods: Immunohistochemistry and Real-time PCR were used to verify the expression of CYP2S1. Colony formation and Transwell assays were used to determine cell proliferation, invasion and migration. Xenograft assays were used to detected cell growth in vivo.

Results: CYP2S1 is significantly up-regulated in lung cancer tissues and cells. Knockdown CYP2S1 in lung cancer cells resulted in decrease cell proliferation, invasion and migration in vitro. Animal experiments showed downregulation of CYP2S1 inhibited lung cancer cell growth in vivo. GSEA analysis suggested that CYP2S1 played functions by regulating E2F targets and G2M checkpoint pathway which involved in cell cycle. Kaplan-Meier analysis indicated that patients with high CYP2S1 had markedly shorter event overall survival (OS) time.

Conclusions: Our data demonstrate that CYP2S1 exerts tumor suppressor function in lung cancer. The high expression of CYP2S1 is an unfavorable prognostic marker for patient survival.
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http://dx.doi.org/10.3233/CBM-210189DOI Listing
July 2021

Single-plasmid systems based on CRISPR-Cas9 for gene editing in Lactococcus lactis.

J Dairy Sci 2021 Jul 15. Epub 2021 Jul 15.

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address:

Lactococcus lactis is a food-grade lactic acid bacterial species that is widely used in food and medical industries. Due to its relatively small genome and simple metabolism, L. lactis is commonly engineered to produce large quantities of recombinant proteins. The most common single-gene knockout strategy in L. lactis involves RecA-dependent homologous double-crossover recombination, which is relatively time-consuming and laborious. In this study, a precise and efficient genome-editing plasmid for L. lactis NZ9000 genome engineering, pLL, was established based on clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology. By studying the effects of different single guide RNA (sgRNA) promoters, the efficiency of gene deletion was optimized. For LLNZ_02045 (ldh), gene deletion efficiency of up to 50% was achieved. Effective sequential gene deletion of LLNZ_11240 (upp) and LLNZ_04580 (upp1) was also demonstrated using this tool. Additionally, the gene that encodes for uracil phosphoribosyltransferase was identified using this system. Similar robust gene deletion efficiencies of sgRNA that targeted different regions of a single gene suggested that gene deletion was not affected by the location of sgRNA binding. Thus, our study established a new gene-editing tool that may allow further investigation and understanding of the L. lactis NZ9000 genome.
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http://dx.doi.org/10.3168/jds.2020-19901DOI Listing
July 2021

Association between homocysteine and third ventricle dilatation, mesencephalic area atrophy in Parkinson's disease with cognitive impairment.

J Clin Neurosci 2021 Aug 21;90:273-278. Epub 2021 Jun 21.

Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China.

Objectives: This study aimed to explore the association of homocysteine (Hcy) with third ventricle (V3) dilatation and mesencephalic area (MA) atrophy as determined by transcranial sonography (TCS) in Parkinson's disease (PD) with cognitive impairment.

Methods: The final statistical analysis included 101 PD patients and 20 age- and sex-matched controls. Using the Movement Disorder Society (MDS) level II criteria for PD with cognitive impairment, we categorized the PD patients into PD with normal cognition group (PD) and PD with cognitive impairment group (PDC). All subjects underwent TCS and laboratory analysis.

Results: The V3 width (r = 0.349, P = 0.005) and the MA (r = -0.484, P < 0.001) were significantly correlated with the Hcy concentration in the PDC patients. Binary logistic regression analysis revealed that age (OR [95% CI] = 1.114 [0.991-1.251], P = 0.002), and Hcy level (OR [95% CI] = 0.931 [0.752-1.153], P = 0.411) were independent risk factors for V3 dilatation. Hcy level (OR [95% CI] = 0.557 [0.323-0.967], P = 0.035) were independent risk factors for MA atrophy. After adjustment for confounding factors, the odds ratio of V3 dilatation was 3.50 (95% CI 1.054-11.399, P = 0.031) and the odds ratio of MA atrophy was 4.67 (95% CI 1.395-15.602, P = 0.012) in the patients with higher Hcy level compared with the lower level.

Conclusions: The results revealed a close association between the V3 width, MA and Hcy concentration in PD patients with cognitive impairment. We hypothesized that increased Hcy concentration played a significant role in the development of brain atrophy in PD with cognitive impairment.
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http://dx.doi.org/10.1016/j.jocn.2021.06.006DOI Listing
August 2021

Genome-wide identification and expression analysis of glycosyltransferase gene family 1 in Quercus robur L.

J Appl Genet 2021 Jul 9. Epub 2021 Jul 9.

College of Life Science, North China University of Science and Technology, Tangshan, Hebei, China.

Glycosyltransferase gene family 1, also known as uridine diphosphate glycosyltransferase (UGT), is the largest glycosyltransferase family in plants, playing a vital role in their growth and development. In this study, 244 UGT genes with conserved PSPG motifs were identified in the genome of Quercus robur L. The collinearity analysis results showed that tandem repeat was the main way of UGT genes expansion in Q. robur, with 21 groups of 55 tandem repeat genes. UGT genes were divided into 15 subgroups A-P; group K was lost, and the gene structure and conserved domain of the same subgroup were basically the same. Cis-element analysis showed that upstream 2,000 bp promoter sequence of UGT genes contained light response elements, plant hormone response elements, and stress-related cis-elements, which indicated that UGT genes of Q. robur might be regulated by various metabolic pathways. In particular, some UGTs in group L of Q. robur contained a conserved promoter structure. The expression pattern analysis results demonstrated that UGT genes of groups B, D, E, and I were differentially expressed under Tortrix viridana L. stress. The expression of UGTs in group E decreased under stress, the expression of group L increased, and that of genes in groups D and B were different. The functions of UGT genes in E and L groups are relatively conservative, and their functions may also conserve among species. The study results have a particular reference value for further research on the function of Q. robur UGT genes.
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http://dx.doi.org/10.1007/s13353-021-00650-3DOI Listing
July 2021

Flexible, superhydrophobic and multifunctional carbon nanofiber hybrid membranes for high performance light driven actuators.

Nanoscale 2021 Jul;13(27):12017-12027

Guangling College, Yangzhou University, Yangzhou, Jiangsu 225009, P. R. China.

Recently, a series of super-hydrophobic materials have been prepared and efforts have been made to further expand their applications, especially in electronics and smart actuators. However, it remains challenging to develop light weight, flexible and super-hydrophobic materials integrating multifunctionalities such as superior photothermal conversion, corrosion resistance, and controllable actuation. Herein, a superhydrophobic and multi-responsive carbon nanofiber (CNF) hybrid membrane with an outstanding photo-thermal effect is fabricated by electrospinning the mixture of polyacrylonitrile and nickel acetylacetonate, followed by two step heat treatment and subsequent fluorination. The superhydrophobic CNF hybrid membrane with outstanding anti-corrosion and self-cleaning performance can float on the water surface spontaneously, thus effectively reducing the motion resistance. The light driven actuation with controllable movement can be achieved by adjusting the laser irradiated location, in which the localized absorption of light is transformed into thermal energy, and hence an imbalanced surface tension is created. The multifunctional hybrid membrane also opens up an arena of applications such as freestanding flexible electronics, drug delivery, and environmental protection.
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http://dx.doi.org/10.1039/d1nr02254gDOI Listing
July 2021

Expression of the CLCA4 Gene in Esophageal Carcinoma and Its Impact on the Biologic Function of Esophageal Carcinoma Cells.

J Oncol 2021 4;2021:1649344. Epub 2021 Jun 4.

Department of General Surgery, Qingdao Hospital of Traditional Chinese Medicine, Qingdao Hiser Hospital, Qingdao 266000, China.

Background: Esophageal carcinoma (ESCA) is one of the malignant tumors with a high mortality rate worldwide, which seriously affects people's health. Calcium-activated chloride channel 4 (CLCA4) was reported to be a tumor inhibitor in hepatocellular carcinoma. Nevertheless, the role of CLCA4 in ESCA is still unclear.

Methods: RT-qPCR and western blot assay were used to test the expression pattern of CLCA4 in ESCA tissues and cells. CCK-8 assay was performed to detect the effect of CLCA4 overexpression on cell proliferation in ESCA cells. Transwell assay was used to measure the effect of CLCA4 upregulation on migration and invasion abilities of ESCA cells. Animal experiments were conducted to investigate the role of CLCA4 upregulation in tumor growth in vivo.

Results: CLCA4 was significantly reduced in ESCA tissues and correlated with stage, differentiation, and lymph node metastasis. CLCA4 overexpression was found to inhibit cell proliferation, migration, invasion, and EMT progression in ESCA cells. Moreover, CLCA4 overexpression suppressed tumor growth in vivo.

Conclusion: CLCA4 was suggested to act as a tumor inhibitor in ESCA and might be a therapeutic target gene for the treatment of patients with ESCA.
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http://dx.doi.org/10.1155/2021/1649344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203369PMC
June 2021

Rapid isolation of exopolysaccharide-producing Streptococcus thermophilus based on molecular marker screening.

J Sci Food Agric 2021 Jun 25. Epub 2021 Jun 25.

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China.

Background: As a natural food additive, exopolysaccharide (EPS) produced by Streptococcus thermophilus can improve product viscosity and texture. The protein EpsA is a putative pathway-specific transcriptional regulator for EPS biosynthesis in S. thermophilus.

Results: According to comparative analysis of EPS biosynthetic gene clusters, a conserved region of epsA (609 bp) was employed to design primer pair epsA-F/R as a molecular marker for the isolation of EPS-producing (EPS ) S. thermophilus. Two EPS S. thermophiles strains, AR333 and S-3, were band-positive, whereas Lactococcus lactis NZ9000 (non-EPS-producing, EPS ), Lactobacillus casei LC2W (EPS ) and L. plantarum AR113 (EPS ) were negative by polymerase chain reaction (PCR) amplicon bands using the epsA probe. This indicated good specificity of the epsA probe to EPS S. thermophilus. Moreover, based on PCR screening with the epsA probe, 23 positive strains were isolated and identified as S. thermophilus from our microbial library and natural fermented milk with 141.3-309.2 mg L of EPS production, demonstrating the validity of our molecular marker screening method.

Conclusion: The designed molecular marker of epsA can rapidly screen EPS S. thermophilus, which has potential application in the dairy and other food industries. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11398DOI Listing
June 2021

The Potential of as a Pigment-Stabilizer to Improve the Pigments Preservation, Flavor Profiles, and Sensory Characteristic of Huangjiu.

Front Microbiol 2021 20;12:678903. Epub 2021 May 20.

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China.

Hong Qu Huangjiu (HQW) is distinguished by its inclusion of pigments, meaning that photosensitivity strongly affects the sensory quality of the wine. In this study, the effects of () on the stability of pigments, the flavor profiles, and the sensory characteristics of HQW were investigated. After sterilization, the addition of increased the preservation rate of pigments in HQW by up to 93.20%, which could be accounted for by the synergy of rutin and quercetin in . The total content of the volatile flavor compounds in HQW increased significantly as the added amounts of were increased, especially 3-methyl-1-butanol, 2-methyl-1-propanol, and short-chain fatty acid ethyl esters (SCFAEE). Sensory evaluation and partial least-squares regression revealed that the concentration of significantly affected the aroma characteristics of HQW but had little effect on the mouthfeel. The addition of 0.9 mg/mL yielded a satisfactory HQW with high scores in terms of mouthfeel and aroma. The strong correlation between fruit-aroma, full-body, and SCFAEE suggests that might alter the aroma of HQW by enhancing the synthesis of SCFAEE. Summarily, treatment with represents a new strategy for improving the stability of photosensitive pigments and thus adjusting the aroma of HQW or similar beverages.
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http://dx.doi.org/10.3389/fmicb.2021.678903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174305PMC
May 2021

Copper Preserves Vasculature Structure and Function by Protecting Endothelial Cells from Apoptosis in Ischemic Myocardium.

J Cardiovasc Transl Res 2021 May 17. Epub 2021 May 17.

Regenerative Medicine Research Center, Sichuan University West China Hospital, Chengdu, Sichuan, 610041, People's Republic of China.

The present study was undertaken to investigate whether Cu protects vasculatures from ischemic injury in the heart. C57/B6 mice were introduced to myocardial ischemia (MI) by permanent ligation of the left anterior descending (LAD) coronary artery. Two hours post-LAD ligation, mice were intravenously injected with a Cu-albumin (Cu-alb) solution, or saline as control. At 1, 4, or 7 days post-MI, hearts were collected for further analysis. A dramatic decrease in CD31-positive endothelial cells concomitantly with abundant apoptosis, along with obstruction of blood flow, was observed in ischemic myocardium 1 day post-MI. The early Cu-alb treatment protected CD31-positive cells from apoptosis, along with a preservation of micro-vessels and a decrease in infarct size. This early vasculature preservation ensured myocardial blood perfusion and protected cardiac contractile function until 28 days post-MI. This strategy of Cu-alb treatment immediately following MI would help develop a therapeutic approach for acute heart attack patients in a clinical setting.
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http://dx.doi.org/10.1007/s12265-021-10128-6DOI Listing
May 2021

Influence of drying and calcination temperatures for Ce-Cu-Al trimetallic composite catalyst on simultaneous removal HS and PH: Experimental and DFT studies.

J Environ Sci (China) 2021 Jun 23;104:277-287. Epub 2020 Dec 23.

Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China. Electronic address:

This work explored the influences of the drying and calcination temperatures on a Ce-Cu-Al trimetallic composite catalyst for the simultaneous removal of HS and PH. The effects of both temperatures on the structural features and activity were examined. The density functional theory method was used to calculate adsorption energies and further analyze their adsorption behavior on different slabs. Experiments revealed suitable drying and calcination temperatures to be 60 and 500°C, respectively. The capacity reached 323.8 and 288.1 mg/g. Adjusting drying temperature to 60°C is more inclined to form larger and structured grains of CuO. Rising calcinating temperature to 500°C could increase the grain size and redox capacity of CuO to promote performance. Higher temperatures would destroy the surface structure and lead to a crystal phase transformation, which was that the CuO and AlO were gradually recombined into CuAlO with a spinel structure. The exposed crystal planes of surficial CuO and CuAlO were determined according to characterization results. Calculation results showed that, compared with CuO (111), HS and PH have weaker adsorption strength on CuAlO (100) which is not conducive to their adsorption and removal.
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http://dx.doi.org/10.1016/j.jes.2020.12.001DOI Listing
June 2021

Recent Research Advances in Small Regulatory RNAs in Streptococcus.

Curr Microbiol 2021 Jun 7;78(6):2231-2241. Epub 2021 May 7.

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Small non-coding RNAs (sRNAs) are a class of regulatory RNAs 20-500 nucleotides in length, which have recently been discovered in prokaryotic organisms. sRNAs are key regulators in many biological processes, such as sensing various environmental changes and regulating intracellular gene expression through binding target mRNAs or proteins. Bacterial sRNAs have recently been rapidly mined, thus providing new insights into the regulatory network of biological functions in prokaryotes. Although most bacterial sRNAs have been discovered and studied in Escherichia coli and other Gram-negative bacteria, sRNAs have increasingly been predicted and verified in Gram-positive bacteria in the past decade. The genus Streptococcus includes many commensal and pathogenic Gram-positive bacteria. However, current understanding of sRNA-mediated regulation in Streptococcus is limited. Most known sRNAs in Streptococcus are associated with the regulation of virulence. In this review, we summarize recent advances in understanding of the functions and mechanisms of sRNAs in Streptococcus, and we discuss the RNA chaperone protein and synthetic sRNA-mediated gene regulation, with the aim of providing a reference for the study of microbial sRNAs.
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http://dx.doi.org/10.1007/s00284-021-02484-yDOI Listing
June 2021

Study on Corrosion Mechanism of Different Concentrations of NaSO Solution on Early-Age Cast-In-Situ Concrete.

Materials (Basel) 2021 Apr 16;14(8). Epub 2021 Apr 16.

Shanxi Qinhan Hengsheng New Building Materials Science and Technology Ltd., Xianyang 712000, China.

The deterioration of early-age concrete performance caused by SO internal diffusion in concrete is a critical factor of concrete durability. In this study, the mechanical properties, heat of hydration, and pore structure of early-age cast-in-situ concrete with different sodium sulfate (NaSO) concentrations were studied. The mechanism of SO internal corrosion was evaluated by measuring the dynamic elastic modulus, compressive strength, and heat of hydration rate. Scanning electron microscopy, energy dispersive spectroscopy, X-ray computed tomography, X-ray diffraction, thermogravimetry-derivative thermogravimetry, and differential scanning calorimetry were applied to analyze microstructural variations and complex mineral assemblages of concrete samples. The results indicated that during the hardening process of cast-in-situ concrete, NaSO first promoted and then hindered the hydration rate of cement, and also hindered the early strength development of the cement. As the concentration of NaSO solution increases, the corrosion products of ettringite (AFt) and gypsum (Gyp) gradually increase, causing cross cracks in the concrete. The proportion of small and medium pores first increases and then decreases, and the large pores first decrease and then increase. The mechanical properties of concrete gradually decrease and diminish the mechanical properties of the concrete (thereby accelerating the damage to the concrete).
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http://dx.doi.org/10.3390/ma14082018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073549PMC
April 2021

Effects of aromatherapy on sleep disorders: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(17):e25727

Institute of Health Preservation, Jiangxi University of Traditional Chinese Medicine Nanchang 330006, Jiangxi Province, China.

Background: The progress of modern society and changes in lifestyle have not only increased the proportion of sub-healthy people, but also caused many people to suffer from sleep disorders and mental anxiety. Long-term lack of high-quality sleep will not only cause psychological problems such as anxiety and fatigue, but also cause physical abnormalities, such as abnormal hormone secretion, weakened immunity, neuroendocrine disorders, and high blood pressure. Therefore, the purpose of this study is to systematically evaluate the effectiveness of aromatherapy in improving sleep quality in people with sleep disorders.

Methods: Computer search CNKI, SinoMed, Wanfang, PubMed, Web of science, and EMbase collect randomized controlled trials on aromatherapy to improve sleep quality of people with sleep disorders. The search time limit is to build the database until April 5, 2021. RevMan5.3 software is used for meta-analysis.

Results: This systematic review will provide an assessment of the current state of sleep disorders, aiming to assess the efficacy of aromatherapy for patients with sleep disorders.

Conclusion: This systematic review will provide a credible evidence-based for the clinical treatment of sleep disorders with aromatherapy.
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http://dx.doi.org/10.1097/MD.0000000000025727DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084014PMC
April 2021

Hepatocyte Growth Factor-Induced Tendon Stem Cell Conditioned Medium Promotes Healing of Injured Achilles Tendon.

Front Cell Dev Biol 2021 7;9:654084. Epub 2021 Apr 7.

Department of Pediatric Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Tendon repair is a medical challenge. Our present study investigated the effectiveness of acellular therapy consisting of conditioned medium (CM) of tendon stem cells (TSCs) induced with hepatocyte growth factor (HGF) in promoting the healing of injured Achilles tendon in a rat model. Proteomic analysis of soluble substances in the CM was performed using an array chip, and bioinformatic analysis was carried out to evaluate interactions among the factors. The effects of CM on viability and migratory capacity of tendon fibroblasts derived from rats with ruptured Achilles tendon were evaluated with the Cell Counting Kit 8 and wound healing assay, respectively. The expression of extracellular matrix (ECM)-related protein was assessed by western blotting. Rats with Achilles tendon injury were treated with CM by local injection for 2 weeks, and the organization of tendon fibers at the lesion site was evaluated by hematoxylin and eosin and Masson's trichrome staining of tissue samples. The deposition and degradation of ECM proteins and the expression of inflammatory factors at the lesion site were evaluated by immunohistochemistry and immunofluorescence. Biomechanical testing was carried out on the injured tendons to assess functional recovery. There were 12 bioactive molecules in the CM, with HGF as the hub of the protein-protein interaction network. CM treatment enhanced the viability and migration of tendon fibroblasts, altered the expression of ECM proteins, promoted the organization of tendon fibers, suppressed inflammation and improved the biomechanics of the injured Achilles tendon. These results suggest that HGF stimulates the secretion of soluble secretory products by TSCs and CM promotes the repair and functional recovery of ruptured Achilles tendon. Thus, HGF-induced TSC CM has therapeutic potential for the treatment of tendinopathy.
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http://dx.doi.org/10.3389/fcell.2021.654084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059769PMC
April 2021

Relationship between mismatch repair protein, , , gene expression and clinicopathological characteristics in elderly colorectal cancer patients.

World J Clin Cases 2021 Apr;9(11):2458-2468

Department of Pathology, First Medical Center, Chinese People's Liberation Army General Hospital, Beijing 100853, China.

Background: Colorectal cancer (CRC) is common in elderly patients. Mismatch repair (MMR) protein deletion is one of the causes of CRC. The (), , and genes are important gene targets in CRC treatment and are closely related to the prognosis and survival of patients. However, little is known regarding the relationship between the expression of MMR, and the clinicopathological features in CRC patients.

Aim: To analyze the relationship between the expression of MMR, and the clinicopathological features in CRC.

Methods: A total of 327 elderly patients with CRC were enrolled, and immuno-histochemistry was used to detect the MMR protein. Real-time quantitative polymerase chain reaction was used to detect the (), , and genes. The clinicopathological data of the patients were recorded and analyzed by SPSS 19.0 statistical software.

Results: In 327 elderly patients with CRC, the rate of MMR protein loss was 9.79% (32/327), and the deletion rate of four MMR proteins (MSH2, MSH6, MLH1, PMS2) was 1.83% (6/327), 3.06% (10/327), 7.65% (25/327), and 7.65% (25/327), respectively. There were no significant differences between MMR protein deletion and sex, pathological type, tumor morphology, differentiation degree or lymph node metastasis ( > 0.05), but there was a significant difference between MMR protein deletion and tumor diameter and tumor location ( = 0.048/ = 0.000). The mutation rates of the and genes in elderly CRC patients were 44.95% (147/327), 2.45% (8/327), 3.36% (11/327) and 2.75% (9/327), respectively; the gene mutation was closely related to tumor morphology ( = 0.002) but not to other clinicopathological features ( > 0.05), and there were no significant differences between gene mutation and clinicopathological features ( > 0.05). The gene mutation showed a significant difference in pathological type, tumor location, differentiation degree and lymph node metastasis ( < 0.05), but was not correlated with sex, tumor size and tumor morphology ( > 0.05). The gene mutation showed no significant differences in the above clinicopathological characteristics ( > 0.05). Significant differences were observed between MMR protein deletion and , , and gene mutations in elderly CRC patients ( = 0.044, = 0.000, = 0.003, respectively), but there was no significant difference between MMR protein deletion and mutation ( > 0.05).

Conclusion: In elderly CRC patients, the tumor is mainly located in the right colon, and the deletion rate of MMR protein is higher when the tumor diameter is greater than or equal to 5 cm; the deletion rate of MLH1 and PMS2 is more common; the mutation rate of gene is higher than that of the , and genes, the gene mutation has different degrees of correlation with clinicopathological characteristics; when the MMR protein is deleted, the and gene mutations are often present, and the gene mutation rate is low.
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http://dx.doi.org/10.12998/wjcc.v9.i11.2458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040173PMC
April 2021

Reply to Zhao: The demonstrated magnitude of artifact during stem water extraction signals a clear need for deuterium correction.

Proc Natl Acad Sci U S A 2021 Apr;118(15)

Isotopomics in Chemical Biology, School of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, 710021 Xi'an, China.

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http://dx.doi.org/10.1073/pnas.2102585118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054005PMC
April 2021

Seasonal variations in water flux compositions controlled by leaf development: isotopic insights at the canopy-atmosphere interface.

Int J Biometeorol 2021 Apr 13. Epub 2021 Apr 13.

State Key Laboratory of Earth Surface Processes and Resource Ecology, Faculty of Geographical Science, Beijing Normal University, Beijing, 100875, China.

Water-stable isotopes provide a valuable tool for tracing plant-water interactions, particularly evapotranspiration (ET) partitioning and leaf water dynamics at the plant-atmosphere interface. However, process-based investigations of plant/leaf development and the associated isotopic dynamics of water fluxes involving isotope enrichment at plant-atmosphere interfaces at the ecosystem scale remain challenging. In this study, in situ isotopic measurements and tracer-aided models were used to study the dynamic interactions between vegetation growth and the isotopic dynamics of water fluxes (ET, soil evaporation, and transpiration) involving isotope enrichment in canopy leaves in a multispecies grassland ecosystem. The day-to-day variations in the isotopic compositions of ET flux were mainly controlled by plant growth, which could be explained by the significant logarithmic relationship determined between the leaf area index and transpiration fraction. Leaf development promoted a significant increase in the isotopic composition of ET and led to a slight decrease in the isotopic composition of water in canopy leaves. The transpiration (evaporation) isoflux acted to increase (decrease) the δO of water vapor, and the total isoflux impacts depended on the seasonal tradeoffs between transpiration and evaporation. The isotopic evidence in ET fluxes demonstrates the biotic controls on day-to-day variations in water/energy flux partitioning through transpiration activity. This study emphasizes that stable isotopes of hydrogen and oxygen are effective tools for quantitative evaluations of the hydrological component partitioning of ecosystems and plant-climate interactions.
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http://dx.doi.org/10.1007/s00484-021-02126-9DOI Listing
April 2021

Reply to Evaristo et al.: Strong evidence for the need of correcting extraction bias in an early study of ecohydrological separation.

Proc Natl Acad Sci U S A 2021 Apr;118(17)

Isotopomics in Chemical Biology, School of Chemistry & Chemical Engineering, Shaanxi University of Science & Technology, 710021 Xi'an, China.

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http://dx.doi.org/10.1073/pnas.2103604118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092563PMC
April 2021

Boronic Acid-Functionalized Scholl-Coupling Mesoporous Polymers for Online Solid-Phase Extraction of Brassinosteroids from Plant-Derived Foodstuffs.

J Agric Food Chem 2021 Apr 13;69(16):4883-4893. Epub 2021 Apr 13.

School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.

Brassinosteroids (BRs) are natural, nontoxic, non-hazardous, biosafe, and eco-friendly plant hormones, possessing diverse pharmacological activities. However, little is known about the type and content of BRs in frequently consumed plant-derived foodstuffs because of their low abundance and high abundance of interference. In this study, a selective, accurate, and sensitive method based on the online solid-phase extraction using the boronic acid-functionalized Scholl-coupling microporous polymer was developed for the analysis of BRs in plant-derived foodstuffs. Under optimum conditions, an excellent linearity ( ≥ 0.9970) and lower limits of detection (0.010-0.070 pg mL) were obtained. The high relative recoveries were in the range of 90.33-109.34% with relative standard deviations less than 9.73%. The method was successfully used for the determination of BRs in fifteen plant-derived foodstuffs. The present work offers a valuable tool for exploring BRs from the plant-derived foodstuffs and can provide useful information for developing functional foods.
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http://dx.doi.org/10.1021/acs.jafc.1c00211DOI Listing
April 2021

Investigation into molecular mechanisms and high-frequency core TCM for pulmonary fibrosis secondary to COVID-19 based on network pharmacology and data mining.

Ann Palliat Med 2021 Apr 1;10(4):3960-3975. Epub 2021 Apr 1.

Department of Pharmacy, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Background: The complication, pulmonary fibrosis (PF) secondary to COVID-19, may have a second wave of late mortality, given the huge number of individuals infected by COVID-19. However, the molecular mechanisms of PF secondary to COVID-19 haven't been fully elucidated, making the identification of novel strategies for targeted therapy challenging. This study aimed to systematically identify the molecular mechanisms and high-frequency core traditional Chinese medicine (TCM) targeting PF secondary to COVID-19 through network pharmacology and data mining.

Methods: The molecular mechanisms of PF secondary to COVID-19 were identified by mapping the COVID-19 differentially expressed gene and known targets associated with PF, protein-protein interactions network analysis, and enrichment pathway analysis; then the high-frequency core TCM targeting PF secondary to COVID-19 were identified by data mining and "Key targets related to PF secondary to COVID-19 - Ingredients" and "Key ingredients-key herbs" network analysis; and last we validated the interaction between the key ingredients and key targets by molecular docking.

Results: The molecular mechanisms of PF secondary to COVID-19 were mainly related to tumor necrosis factor (TNF) signaling pathway, cytokine-cytokine receptor interaction pathway, and NF-κB signaling pathway. Among these, cytokines interleukin 6 (IL-6), TNF, and IL-1β were identified as the key targets associated with PF secondary to COVID-19. The high-frequency core TCM targeting these key targets were identified, including ingredients of quercetin, epigallocatechin-3-gallate, emodin, triptolide, resveratrol, and herb of Polygonum cuspidatum. Finally, our results were validated by quercetin and resveratrol both well docked to IL-6, TNF, and IL-1β protein, with the estimated docking energy <0 kcal/mol.

Conclusions: This study identified the cytokines-related molecular mechanisms of PF secondary to COVID-19, and the high-frequency core TCM against PF by targeting IL-6, TNF, and IL-1β. Which provides new ideas for the discovery of small molecular compounds with potential therapeutic effects on PF secondary to COVID-19.
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http://dx.doi.org/10.21037/apm-20-1384DOI Listing
April 2021

Functional Studies of Novel FOXL2 Variants in Chinese Families With Blepharophimosis-Ptosis-Epicanthus Inversus Syndrome.

Front Genet 2021 16;12:616112. Epub 2021 Mar 16.

Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) is a rare autosomal dominant disease mainly caused by FOXL2 variants. This genetic disorder is usually characterized by eyelid malformation and ovarian dysfunction. However, no reliable genotype/phenotype correlations have been established considering the ovarian phenotype. Here, we detected 15 FOXL2 variants including nine novel ones from 7 families and 8 sporadic cases, which expanded the spectrum of FOXL2 variants and identified a potential clinical cause. Functional studies, with respect to the effect of FOXL2 on the StAR promoter, showed that non-sense variants that lead to protein truncation before the polyalanine tract and missense variants [c.307C > T; p.(Arg103Cys), c.311A > C; p.(His104Pro), c.320G > A; p.(Ser107Asn), and c.335T > A; p.(Phe112Tyr)] within the central portion of the FOXL2 forkhead domain significantly affect its suppressor activity. Such changes may explain the mechanism underlying a more severe phenotype, more likely to result in BPES type I. Furthermore, the missenses variants c.307C > T; p.(Arg103Cys), c.311A > C; p.(His104Pro), and c.320G > A; p.(Ser107Asn) were not able to transactivate OSR2, which is consistent with the eyelid malformation in these patients. The results from our cohort have expanded the spectrum of FOXL2 variants and have provided insights into genotype/phenotype correlations.
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http://dx.doi.org/10.3389/fgene.2021.616112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007913PMC
March 2021

Assessing the relationship between the human learned helplessness depression model and anhedonia.

PLoS One 2021 30;16(3):e0249056. Epub 2021 Mar 30.

Department of Psychology, University of Minnesota, Minneapolis, Minnesota, United States of America.

The learned helplessness (LH) model is one of the most commonly used acute stress models to explain depression and it has shown good face and predictive validity. However, despite being able to induce depressed-like behaviors and corresponding psychophysiological changes, there is little evidence showing that the LH paradigm can produce anhedonia, a core symptom seen in all forms of depression in humans. So far a couple of studies showed that rodents bred for helplessness develop anhedonic-like behaviors in response to stress; yet, to the best of our knowledge, no similar human research has tried to investigate the direct relationship between the LH model and anhedonia. In the present study, we use a modified version of the original LH task to experimentally and temporarily induce learned helplessness in college students and then examine if the human LH paradigm induces anhedonia. We aim to 1: address the ill-defined connection between the LH model and anhedonia, and 2: directly assess helplessness in humans as opposed to the majority of non-human animal subjects used in the helplessness literature. We believe that our study will fill an important gap in the learned helplessness model literature, and will advance our understanding of the relationship between depression and perceived control, as well as place limitations to what can and cannot be inferred from non-human animal data in this topic.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249056PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009390PMC
March 2021

Bromodomain-containing proteins BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and vital for their genomic targeting in Arabidopsis.

Mol Plant 2021 Jun 24;14(6):888-904. Epub 2021 Mar 24.

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China. Electronic address:

Switch defective/sucrose non-fermentable (SWI/SNF) chromatin remodeling complexes are multi-subunit machines that play vital roles in the regulation of chromatin structure and gene expression. However, the mechanisms by which SWI/SNF complexes recognize their target loci in plants are not fully understood. Here, we show that the Arabidopsis thaliana bromodomain-containing proteins BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and critical for SWI/SNF genomic targeting. These three BRDs interact directly with multiple SWI/SNF subunits, including the BRAHMA (BRM) catalytic subunit. Phenotypic and transcriptomic analyses of the brd1 brd2 brd13 triple mutant revealed that these BRDs act largely redundantly to control gene expression and developmental processes that are also regulated by BRM. Genome-wide occupancy profiling demonstrated that these three BRDs extensively colocalize with BRM on chromatin. Simultaneous loss of function of three BRD genes results in reduced BRM protein levels and decreased occupancy of BRM on chromatin across the genome. Furthermore, we demonstrated that the bromodomains of BRDs are essential for genomic targeting of the BRD subunits of SWI/SNF complexes to their target sites. Collectively, these results demonstrate that BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and reveal their biological roles in facilitating genomic targeting of BRM-containing SWI/SNF complexes in plants.
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http://dx.doi.org/10.1016/j.molp.2021.03.018DOI Listing
June 2021

Enhanced biostimulation coupled with a dynamic groundwater recirculation system for Cr(VI) removal from groundwater: A field-scale study.

Sci Total Environ 2021 Jun 1;772:145495. Epub 2021 Feb 1.

Nanjing Kangdi Environmental Protection Technology Co., LTD, Nanjing 21000, China.

A large gap exists between laboratory findings and successful implementation of bioremediation technologies for the treatment of chromium (Cr)-contaminated sites. This work conducted the enhanced bioremediation of Cr(VI) in situ via the addition of organic carbon (ethanol) coupled with a dynamic groundwater recirculation (DGR)-based system in a field-scale study. The DGR system was applied to successfully (1) remove Cr(VI) from groundwater via enhanced flushing by the recirculation system and (2) deliver the biostimulant to the heterogeneous subsurface environment, including a sand/cobble aquifer and a fractured bedrock aquifer. The results showed that the combined extraction and bioreduction of Cr(VI) were able to reduce Cr(VI) concentrations from 1000 to 2000 mg/L to below the clean-up goal of 0.1 mg/L within the operation period of 52 days. The effectiveness of Cr(VI) bioremediation and the relationship between microbial communities and geochemical parameters were evaluated. Multiple-line of evidence demonstrated that the introduction of ethanol significantly stimulated a variety of bacteria, including those responsible for denitrification, sulfate reduction and reduction of Cr(VI), which contributed to the establishment of reducing conditions in both aquifers. Cr(VI) was removed from groundwater via combined mechanisms of physical removal through the DGR system and the bioreduction of Cr(VI) followed by precipitation. In particular, it was found competitive growth among Cr(VI)-reducing bacteria (such as the enrichment of Geobacter, along with the reduced relative abundance of Acinetobacter and Pseudomonas) was induced by ethanol injection. Furthermore, Cr(VI), total organic carbon, NO, and SO played important roles in shaping the composition of the microbial community and its functions.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145495DOI Listing
June 2021

SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves.

BMC Neurosci 2021 03 22;22(1):18. Epub 2021 Mar 22.

College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China.

Background: The SCN11A gene, encoded Nav1.9 TTX resistant sodium channels, is a main effector in peripheral inflammation related pain in nociceptive neurons. The role of SCN11A gene in the auditory system has not been well characterized. We therefore examined the expression of SCN11A in the murine cochlea, the morphological and physiological features of Nav1.9 knockout (KO) ICR mice.

Results: Nav1.9 expression was found in the primary afferent endings beneath the inner hair cells (IHCs). The relative quantitative expression of Nav1.9 mRNA in modiolus of wild-type (WT) mice remains unchanged from P0 to P60. The number of presynaptic CtBP2 puncta in Nav1.9 KO mice was significantly lower than WT. In addition, the number of SGNs in Nav1.9 KO mice was also less than WT in the basal turn, but not in the apical and middle turns. There was no lesion in the somas and stereocilia of hair cells in Nav1.9 KO mice. Furthermore, Nav1.9 KO mice showed higher and progressive elevated ABR threshold at 16 kHz, and a significant increase in CAP thresholds.

Conclusions: These data suggest a role of Nav1.9 in regulating the function of ribbon synapses and the auditory nerves. The impairment induced by Nav1.9 gene deletion mimics the characters of cochlear synaptopathy.
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http://dx.doi.org/10.1186/s12868-021-00613-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986359PMC
March 2021

Case Report: Favorable Response to the Tyrosine Kinase Inhibitor Apatinib in Recurrent Merkel Cell Carcinoma.

Front Oncol 2021 3;11:625360. Epub 2021 Mar 3.

Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, China.

Background: As angiogenesis is an essential step in tumor growth and metastasis, the tyrosine kinase inhibitor (TKI) apatinib has become a revolutionary anticancer therapy across various malignancies. However, its efficiency and safety in Merkel cell carcinoma (MCC) are uncertain.

Case Presentation: The current study described the case of a 91-year-old man who presented with a 3.2 × 3.0 × 2.2 cm rapidly growing, solitary tumor of the right lower eyelid. It was diagnosed as MCC pathologically. Twenty-seven days after the surgery, the patient returned to the hospital with recurrent MCC. Apatinib was then administered to this patient. The patient had a complete response (CR) to apatinib after 4.4 months of targeted therapy. Twenty-seven months of progression-free survival (PFS) was achieved with controllable treatment-related adverse events (AEs).

Conclusion: Treatment with apatinib demonstrated clinical benefit in our patient with recurrent MCC, highlighting its potential utility in other MCC patients. Further clinical trials are needed to determine the efficacy and safety of apatinib in MCC patients.
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http://dx.doi.org/10.3389/fonc.2021.625360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966513PMC
March 2021

Human Cytomegalovirus miR-US33as-5p Targets IFNAR1 to Achieve Immune Evasion During Both Lytic and Latent Infection.

Front Immunol 2021 5;12:628364. Epub 2021 Mar 5.

Institute of Health Service and Transfusion Medicine, Academy of Military Medical Sciences, Beijing, China.

As the first line of antiviral defense, type I interferon (IFN) binds IFN receptor 1 (IFNAR1) and IFNAR2 to activate the Jak-STAT signal transduction pathway, producing IFN-stimulated genes (ISGs) to control viral infection. The mechanisms by which human cytomegalovirus (HCMV) counteracts the IFN pathway are only partially defined. We show that miR-US33as-5p encoded by HCMV is expressed in both lytic and latent infection. By analysis with RNA hybrid and screening with luciferase reporter assays, we identified IFNAR1 as a target of hcmv-miR-US33as-5p, which was further verified by examining the expression of two IFNAR1 mutants and the binding of IFNAR1 to miR-US33as-5p/miR-US33as-5p-M1/miR-US33as-5p-M2. We found that after the transfection of miR-US33as-5p mimics into different cell lines, the phosphorylation of downstream proteins and ISG expression were downregulated. Immunofluorescence showed that the miR-US33as-5p mimics also inhibited STAT1 translocation into the nucleus. Furthermore, we constructed HCMV with mutant miR-US33as-5p and determined that the mutation did not affect HCMV replication. We found that MRC-5/human foreskin fibroblast (HFF) cells infected with ΔmiRNA HCMV exhibited higher IFNAR1 and ISG expression and a reduced viral load in the presence of exogenous IFN than cells infected with WT HCMV did, confirming that the knockout of miR-US33as-5p impaired viral resistance to IFN. Finally, we tested the effect of ΔmiRNA HCMV on THP-1 and d-THP-1 cells, common models of latent infection and reactivation, respectively. Again, we found that cells infected with ΔmiRNA HCMV showed a reduced viral load in the presence of IFN than the control cells did, confirming that miR-US33as-5p also affects IFN resistance during both latency and reactivation. These results indicate a new microRNA (miRNA)-based immune evasion mechanism employed by HCMV to achieve lifelong infection.
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http://dx.doi.org/10.3389/fimmu.2021.628364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973039PMC
March 2021

Best Supportive Care Whole-Brain Irradiation, Chemotherapy Alone, or WBRT Plus Chemotherapy in Patients With Brain Metastases From Small-Cell Lung Cancer: A Case-Controlled Analysis.

Front Oncol 2021 1;11:568568. Epub 2021 Mar 1.

Department of Surgery, Shanxi Medical University, Shanxi Cancer Hospital, Taiyuan, China.

Background: WBRT and systemic chemotherapy are the mainstay treatments for small-cell lung cancer (SCLC) brain metastases (BM). However, current recommendations are mainly based on evidence from retrospective analyses. A recent randomized trial found no benefits from WBRT compared with best supportive care (BSC) in patients with more than three BM from non-small-cell lung cancer (NSCLC). Herein, we aimed to evaluate the roles of WBRT and chemotherapy further in the management of BM from SCLC.

Materials And Methods: There were 698 patients with BM from SCLC included. Of these, 580 received anti cancer treatment (Group 1), including 178 who received WBRT only (Group 1a), 129 who received chemotherapy only (Group 1b), and 273 who received WBRT plus chemotherapy (Group 1c). The other 118 received BSC (Group 2). Propensity score matching (PSM) analysis was used to compare Group 2 with each of the other groups.

Results: After PSM, compared with Group 2 (n = 118), patients in Group 1 (n = 440) had a prolonged overall survival (OS) in both univariate and multivariate tests, with a median survival time of 10 months (95% CI = 9-11) in Group 1 and 3.5 months (95% CI = 2-7) in Group 2 (p < 0.001). In subgroup analyses, patients who received WBRT plus chemotherapy were more likely to benefit from treatment (p < 0.001). Chemotherapy alone or WBRT alone did not show survival benefits.

Conclusion: WBRT plus chemotherapy improved OS in patients with BM from SCLC as compared to BSC. Chemotherapy alone and WBRT alone did not show survival benefits. This retrospective study suggests that SCLC patients with BM who receive WBRT combined with chemotherapy have a better outcome than those receiving BSC alone.
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http://dx.doi.org/10.3389/fonc.2021.568568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957068PMC
March 2021

Transforaminal lumbar interbody fusion with reduction of slippage of vertebrae for isthmic lumbar spondylolisthesis: Unilateral versus bilateral interbody fusion.

Clin Neurol Neurosurg 2021 Apr 9;203:106588. Epub 2021 Mar 9.

The Department of Orthopaedics, Shanghai Pudong New District Peoples' Hospital, Shanghai, China. Electronic address:

Objective: To investigate the radiological and clinical efficacy of transforaminal lumbar bilateral interbody fusion (TLBIF) versus transforaminal lumbar unilateral interbody fusion (TLUIF) with reduction of slippage of the vertebra in isthmic lumbar spondylolisthesis (ILS).

Methods: A comparative retrospective study was conducted between patients undergoing TLBIF (n = 46) and TLUIF (n = 40). Demographic data, intraoperative data, complications, and radiographic parameters, including total lumbar lordosis (LL), intervertebral disc height (IDH), and foraminal height (FH), and the final fusion rate were evaluated and compared between the two groups. Clinical outcomes were assessed by the Visual Analog Scale (VAS) for low back pain and leg pain, and the Oswestry Disability Index (ODI).

Results: Significant improvement in terms of radiographic and clinical outcomes was achieved in both groups during the period from pre-operation to at least 24 months post-operation (all, P < 0.05).

Original Sentence: Analysis of back and leg pain by VAS score and radiographic data including LL, IDH and FH showed superior improvement in TLBIF group compared with TLUIF group. However, ODI was similar between the two groups.

Revised: Analysis of radiographic data, including LL, IDH, and FH, showed superior improvement in the TLBIF group compared with the TLUIF group (all, P < 0.05). However, either back and leg pain by the VAS score or ODI was similar between the two groups (all, P > 0.05). The fusion rate was 100 % in the TLBIF group versus 95 % in the TLUIF group during the 24-month follow-up period. The operation time was longer and the complication rate was lower in the TLBIF group than in the TLUIF group (all, P < 0.05), while intraoperative blood loss was similar between the two groups (P > 0.05).

Conclusions: TLBIF with reduction did not cause significant procedure-associated complications in ILS patients. The therapeutic outcome of TLBIF was satisfactory and similar to that of TLUIF for ILS.

Original Sentence: Compared with TLUIF, TLBIF appears to be associated with better radiological data, less postoperative back and leg discomfort, and quicker postoperative recovery.

Revised: Compared with TLUIF, TLBIF appears to be associated with better radiological data, bone fusion rate, and similar patient-reported outcomes (PROs), including the ODI and VAS pain score for the back and leg.
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http://dx.doi.org/10.1016/j.clineuro.2021.106588DOI Listing
April 2021

Antrodin A from Antrodia camphorata modulates the gut microbiome and liver metabolome in mice exposed to acute alcohol intake.

Food Funct 2021 Apr 15;12(7):2925-2937. Epub 2021 Mar 15.

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

This study aimed to investigate the protective effect of Antrodin A (AdA) from Antrodia camphorata (A. camphorata) mycelium on alcohol-induced gut microbiota and liver metabolomic disorders. In acute alcoholic liver injury mice, AdA ameliorated alcoholic exposure-induced hepatic lipid deposition (TC and TG), oxidative stress (MDA), inflammation (TNF-α, IL-1β, IL-6, IL-17 and IFN-γ), and liver damage via modulating microbiome and metabolomic responses. AdA restored the composition of intestinal flora with an increase in the relative abundance of Lactobacillus and Dubosiella and a decrease in Clostridium_sensu_stricto_1, Lachnospiraceae_NK4A136_group, Prevotellaceae_NK3B31_group, and Prevotellaceae_UCG-001. Besides, AdA favorably regulated alcohol-induced metabolic disorders, including glutathione metabolism (S-(2-hydroxyethyl)glutathione and glutathione oxidized), ascorbate and aldarate metabolism (l-ascorbic acid), and taurine and hypotaurine metabolism (taurine). In conclusion, AdA in A. camphorata is a beneficial active ingredient to treat the microbiomic and metabolic disturbance induced by alcohol intake.
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http://dx.doi.org/10.1039/d0fo03345fDOI Listing
April 2021