Publications by authors named "Xin Pan"

460 Publications

Two different protein corona formation modes on Soluplus® nanomicelles.

Colloids Surf B Biointerfaces 2022 Aug 2;218:112744. Epub 2022 Aug 2.

School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, Guangdong, PR China. Electronic address:

Soluplus® nanomicelles have been widely reported in biomedical field for their excellent drug loading capacity and solubility enhancement ability. However, when administrated in vivo, the protein corona will be formed on Soluplus® nanomicelles, significantly affecting their drug delivery performance. Up to now, few studies examined the protein corona formation process and its impact factors of Soluplus® nanomicelles. The multiple proteins in biofluids may form protein corona in different modes due to their diversified properties. In this study, Bovine serum albumin (BSA), Lysozyme (Lyso) and Bovine hemoglobin (BHb) were chosen as model proteins to investigate the protein corona formation process of Soluplus® nanomicelles. By analyzing the polarity of the protein amino acid residues distributing microenvironments, the results showed that there were two different protein corona formation modes, i.e., surface adsorption and insertion, which were determined by the hydrophilicity of proteins. The hydrophobic BHb followed the insertion mode while hydrophilic BSA and Lyso followed the surface adsorption mode. Ultimately, upon protein corona formation, the size and surface chemistry of nanomicelles was significantly affected. We believe this study will provide a new research paradigm to the design and application of Soluplus® nanomicelles.
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http://dx.doi.org/10.1016/j.colsurfb.2022.112744DOI Listing
August 2022

Strategies to Improve the Biosynthesis of β-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects.

Front Bioeng Biotechnol 2022 18;10:936487. Epub 2022 Jul 18.

Department of Cardiology, Central Laboratory, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.

β-Lactam antibiotics are widely used anti-infection drugs that are traditionally synthesized via a chemical process. In recent years, with the growing demand for green alternatives, scientists have turned to enzymatic synthesis. Penicillin G acylase (PGA) is the second most commercially used enzyme worldwide with both hydrolytic and synthetic activities toward antibiotics, which has been used to manufacture the key antibiotic nucleus on an industrial level. However, the large-scale application of PGA-catalyzed antibiotics biosynthesis is still in the experimental stage because of some key limitations, such as low substrate concentration, unsatisfactory yield, and lack of superior biocatalysts. This paper systematically reviews the strategies adopted to improve the biosynthesis of β-lactam antibiotics by adjusting the enzymatic property and manipulating the reaction system in recent 20 years, including mining of enzymes, protein engineering, solvent engineering, product removal, and one-pot reaction cascade. These advances will provide important guidelines for the future use of enzymatic synthesis in the industrial production of β-lactam antibiotics.
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http://dx.doi.org/10.3389/fbioe.2022.936487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340067PMC
July 2022

Bone Tissue Engineering in the Treatment of Bone Defects.

Pharmaceuticals (Basel) 2022 Jul 17;15(7). Epub 2022 Jul 17.

Jiangsu Provincial Engineering Research Center of Traditional Chinese Medicine External Medication Development and Application, Nanjing University of Chinese Medicine, Nanjing 210023, China.

Bones play an important role in maintaining exercise and protecting organs. Bone defect, as a common orthopedic disease in clinics, can cause tremendous damage with long treatment cycles. Therefore, the treatment of bone defect remains as one of the main challenges in clinical practice. Today, with increased incidence of bone disease in the aging population, demand for bone repair material is high. At present, the method of clinical treatment for bone defects including non-invasive therapy and invasive therapy. Surgical treatment is the most effective way to treat bone defects, such as using bone grafts, Masquelet technique, Ilizarov technique etc. In recent years, the rapid development of tissue engineering technology provides a new treatment strategy for bone repair. This review paper introduces the current situation and challenges of clinical treatment of bone defect repair in detail. The advantages and disadvantages of bone tissue engineering scaffolds are comprehensively discussed from the aspect of material, preparation technology, and function of bone tissue engineering scaffolds. This paper also summarizes the 3D printing technology based on computer technology, aiming at designing personalized artificial scaffolds that can accurately fit bone defects.
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http://dx.doi.org/10.3390/ph15070879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324138PMC
July 2022

Evaluation of the Abilities of Three Kinds of Copper-Based Nanoparticles to Control Kiwifruit Bacterial Canker.

Antibiotics (Basel) 2022 Jul 4;11(7). Epub 2022 Jul 4.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals, Guizhou University, Huaxi District, Guiyang 550025, China.

Kiwifruit bacterial canker caused by reduces kiwifruit crop yield and quality, leading to economic losses. Unfortunately, few agents for its control are available. We prepared three kinds of copper-based nanoparticles and applied them to control kiwifruit bacterial canker. The successful synthesis of Cu(OH) nanowires, Cu(PO) nanosheets, and Cu(OH)Cl nanoparticles were confirmed by transmission and scanning electron microscopy, energy dispersive spectroscopy, X-ray diffraction analysis, and X-ray photoelectron spectroscopy. The minimum bactericidal concentrations (MBCs) of the three nanoparticles were 1.56 μg/mL, which exceeded that of the commercial agent thiodiazole copper (MBC > 100 μg/mL). The imaging results indicate that the nanoparticles could interact with bacterial surfaces and kill bacteria by inducing reactive oxygen species' accumulation and disrupting cell walls. The protective activities of Cu(OH) nanowires and Cu(PO) nanosheets were 59.8% and 63.2%, respectively, similar to thiodiazole copper (64.4%) and better than the Cu(OH)Cl nanoparticles (40.2%). The therapeutic activity of Cu(OH)Cl nanoparticles (67.1%) bested that of Cu(OH) nanowires (43.9%), Cu(PO) nanosheets (56.1%), and thiodiazole copper (53.7%). Their therapeutic and protective activities for control of kiwifruit bacterial canker differed in vivo, which was related to their sizes and morphologies. This study suggests these copper-based nanoparticles as alternatives to conventional bactericides for controlling kiwifruit diseases.
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http://dx.doi.org/10.3390/antibiotics11070891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312301PMC
July 2022

Structural and Functional Basis of JAMM Deubiquitinating Enzymes in Disease.

Biomolecules 2022 Jun 29;12(7). Epub 2022 Jun 29.

Department of Cardiology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225000, China.

Deubiquitinating enzymes (DUBs) are a group of proteases that are important for maintaining cell homeostasis by regulating the balance between ubiquitination and deubiquitination. As the only known metalloproteinase family of DUBs, JAB1/MPN/Mov34 metalloenzymes (JAMMs) are specifically associated with tumorigenesis and immunological and inflammatory diseases at multiple levels. The far smaller numbers and distinct catalytic mechanism of JAMMs render them attractive drug targets. Currently, several JAMM inhibitors have been successfully developed and have shown promising therapeutic efficacy. To gain greater insight into JAMMs, in this review, we focus on several key proteins in this family, including AMSH, AMSH-LP, BRCC36, Rpn11, and CSN5, and emphatically discuss their structural basis, diverse functions, catalytic mechanism, and current reported inhibitors targeting JAMMs. These advances set the stage for the exploitation of JAMMs as a target for the treatment of various diseases.
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http://dx.doi.org/10.3390/biom12070910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313428PMC
June 2022

Deoxynivalenol Biosynthesis in Significantly Repressed by a Megabirnavirus.

Toxins (Basel) 2022 Jul 19;14(7). Epub 2022 Jul 19.

Department of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.

Deoxynivalenol (DON) is a mycotoxin widely detected in cereal products contaminated by Fusarium pseudograminearum megabirnavirus 1 (FpgMBV1) is a double-stranded RNA virus infecting . In this study, it was revealed that the amount of DON in was significantly suppressed by FpgMBV1 through a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay. A total of 2564 differentially expressed genes were identified by comparative transcriptomic analysis between the FpgMBV1-containing strain FC136-2A and the virus-free strain FC136-2A-V. Among them, 1585 genes were up-regulated and 979 genes were down-regulated. Particularly, the expression of 12 genes (, , , , , , , , , , and ) in the trichothecene biosynthetic () gene cluster was significantly down-regulated. Specific metabolic and transport processes and pathways including amino acid and lipid metabolism, ergosterol metabolic and biosynthetic processes, carbohydrate metabolism, and biosynthesis were regulated. These results suggest an unrevealing mechanism underlying the repression of DON and gene expression by the mycovirus FpgMBV1, which would provide new methods in the detoxification of DON and reducing the yield loss in wheat.
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http://dx.doi.org/10.3390/toxins14070503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324440PMC
July 2022

Retrograde Approach in Balloon Pulmonary Vein Angioplasty.

JACC Cardiovasc Interv 2022 07 25;15(14):e171-e172. Epub 2022 May 25.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

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http://dx.doi.org/10.1016/j.jcin.2022.04.008DOI Listing
July 2022

"Pincer movement": Reversing cisplatin resistance based on simultaneous glutathione depletion and glutathione -transferases inhibition by redox-responsive degradable organosilica hybrid nanoparticles.

Acta Pharm Sin B 2022 Apr 21;12(4):2074-2088. Epub 2021 Oct 21.

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.

The therapeutic efficacy of cisplatin has been restricted by drug resistance of cancers. Intracellular glutathione (GSH) detoxification of cisplatin under the catalysis of glutathione -transferases (GST) plays important roles in the development of cisplatin resistance. Herein, a strategy of "pincer movement" based on simultaneous GSH depletion and GST inhibition is proposed to enhance cisplatin-based chemotherapy. Specifically, a redox-responsive nanomedicine based on disulfide-bridged degradable organosilica hybrid nanoparticles is developed and loaded with cisplatin and ethacrynic acid (EA), a GST inhibitor. Responding to high level of intracellular GSH, the hybrid nanoparticles can be gradually degraded due to the break of disulfide bonds, which further promotes drug release. Meanwhile, the disulfide-mediated GSH depletion and EA-induced GST inhibition cooperatively prevent cellular detoxification of cisplatin and reverse drug resistance. Moreover, the nanomedicine is integrated into microneedles for intralesional drug delivery against cisplatin-resistant melanoma. The results show that the nanomedicine-loaded microneedles can achieve significant GSH depletion, GST inhibition, and consequent tumor growth suppression. Overall, this research provides a promising strategy for the construction of new-type nanomedicines to overcome cisplatin resistance, which extends the biomedical application of organosilica hybrid nanomaterials and enables more efficient chemotherapy against drug-resistant cancers.
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http://dx.doi.org/10.1016/j.apsb.2021.10.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279704PMC
April 2022

Co-spray-dried poly-L-lysine with L-leucine as dry powder inhalations for the treatment of pulmonary infection: Moisture-resistance and desirable aerosolization performance.

Int J Pharm 2022 Jul 9;624:122011. Epub 2022 Jul 9.

College of Pharmacy, Jinan University, Guangzhou 510632, Guangdong, PR China. Electronic address:

Poly-L-lysine (PLL) is a promising candidate for the treatment of pulmonary infection with lower occurrence of drug-resistance due to its unique antibacterial mechanisms. Dry powder inhalations (DPIs) are considered as the first choice for formulating PLL to treat pulmonary infection on account of direct delivery and satisfactory stability. However, hygroscopicity of PLL limited its therapeutic effect on pulmonary infection when PLL developed into DPIs. The hygroscopicity caused two obstacles including the low drug deposition in the lower respiratory tract and undesirable aerosolization performance deterioration. In this study, PLL was co-spray-dried with L-leucine (LL) to achieve moisture-resistance and desirable aerosolization performance. The ratio of PLL and LL was optimized to obtain particles with different morphology, hygroscopicity and aerodynamic properties. The obtained PLL DPIs were suitable for inhalation with a corrugated surface formed by hydrophobic LL. The anti-hygroscopicity, aerosolization performance and rheological properties of P DPIs were optimal when PLL:LL = 85:15. The DPIs particles were stable after being stored at high relative humidity (60 ± 5%), and their superiority in treating pulmonary infections was also proved by in vitro and in vivo experiments. The established PLL DPIs were proved to be a feasible and desirable approach to treat pulmonary infections.
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http://dx.doi.org/10.1016/j.ijpharm.2022.122011DOI Listing
July 2022

Pannus formation and iatrogenic ventricular septal defect after transcatheter aortic valve implantation.

Eur Heart J Case Rep 2022 Jul 28;6(7):ytac265. Epub 2022 Jun 28.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 241 West Huaihai Road, Shanghai 200030, China.

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http://dx.doi.org/10.1093/ehjcr/ytac265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263154PMC
July 2022

Amelioration of Coastal Salt-Affected Soils with Biochar, Acid Modified Biochar and Wood Vinegar: Enhanced Nutrient Availability and Bacterial Community Modulation.

Int J Environ Res Public Health 2022 06 14;19(12). Epub 2022 Jun 14.

Institute of Coastal Environmental Pollution Control, Key Laboratory of Marine Environment and Ecology, Ministry of Education, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266100, China.

As an important part of the ecological environment, degraded coastal soils urgently require efficient and eco-friendly soil amendment. Biochar and wood vinegar have been proved to be effective soil amendments, and acid-modified biochar has great potential in ameliorating the degraded coastal saline-alkali soil. However, the effects of individual or combined application of biochar (BC), acid-modified biochar (ABC), and wood vinegar (WV) on coastal saline-alkali soil are unknown. Hence, biochar, wood vinegar, and acid-modified biochar were prepared by pyrolysis of poplar wood. The properties of biochar were characterized, and soil incubation experiments were conducted. The results showed that ABC decreased the soil alkalinity by acid-base neutralization and improved the soil fertility by increasing the nutrients (C, N, P). ABC provided a more suitable environment and changed the abundance and diversity of soil microorganisms. ABC increased the relative contents of specific families (e.g., and ), which had strong ecological linkages in the C, N, and P cycles and organic matter degradation. The results indicated that WV had little effect on coastal saline-alkali soil, whereas individual and combined application of biochar (especially ABC) showed an efficient remediation effect. Our preliminary study demonstrated that the ABC could be a suitable solution for ameliorating degraded coastal saline-alkali soils.
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http://dx.doi.org/10.3390/ijerph19127282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223450PMC
June 2022

Genetic polymorphisms in DNA repair genes and their association with risk of cervical cancer: A systematic review and meta-analysis.

J Obstet Gynaecol Res 2022 Jun 22. Epub 2022 Jun 22.

Institute of Traditional Chinese Medicine Literature, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.

Background: There have been a large number of epidemiologic studies regarding the association between genetic polymorphisms in DNA repair genes and onset of cervical cancer. However, results are inconsistent.

Methods: Articles published before June 2021 and regarding genetic polymorphisms in DNA repair genes and cervical cancer were searched in following databases: PubMed, Web of Science, Google Scholar, and CNKI. With at least three articles for each polymorphism, we made meta-analysis to compute multivariate odds ratios (ORs) and their 95% confidence intervals (CIs).

Results: The present study showed significant associations between XRCC1 Arg399Gln polymorphisms and risk of cervical cancer in Asian, whereas no significant association between them were showed in Caucasian (Asian: GA vs. GG: OR = 1.27, 95%CI 1.06-1.52; AA vs. GG: OR = 1.91, 95%CI 1.29-2.83; GA + AA vs. GG: OR = 1.36, 95%CI 1.12-1.65; AA vs. GG + GA: OR = 1.66, 95%CI 1.17-2.37; Caucasian: GA vs. GG: OR = 1.08, 95%CI 0.83-1.41; AA vs. GG: OR = 2.18, 95%CI 0.75-6.31; GA + AA vs. GG: OR = 1.23, 95%CI 0.85-1.78; AA vs. GG + GA: OR = 1.70, 95%CI 0.69-4.18). In addition, there were significant associations between ERCC2 rs13181 polymorphisms and risk of cervical cancer in Asian (AC vs AA: OR = 0.53, 95%CI 0.37-0.75, I  = 0.0%, p value of Q test = 0.847; AC + CC vs AA: OR = 0.50, 95%CI 0.36-0.70, I  = 0.0%, p value of Q test = 0.856).

Conclusions: The meta-analysis showed that there were significant associations between XRCC1 Arg399Gln and ERCC2 rs13181 polymorphisms and risk of cervical cancer.
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http://dx.doi.org/10.1111/jog.15325DOI Listing
June 2022

Dry Powder Inhalers Based on Chitosan-Mannitol Binary Carriers: Effect of the Powder Properties on the Aerosolization Performance.

AAPS PharmSciTech 2022 Jun 14;23(5):164. Epub 2022 Jun 14.

College of Pharmacy, Jinan University, Guangzhou, 510632, Guangdong, People's Republic of China.

Carriers play an important role in improving the aerosolization performance of dry powder inhalers (DPIs). Despite that intensive attention had been paid to the establishment of the advanced carriers with controllable physicochemical properties in recent years, the design and optimization of carrier-based DPIs remain an empiricism-based process. DPIs are a powder system of complex multiphase, and thus their physicochemical properties cannot fully explain the powder behavior. A comprehensive exposition of powder properties is demanded to build a bridge between the physicochemical properties of carriers and the aerosolization performance of DPIs. In this study, an FT-4 powder rheometer was employed to explore the powder properties, including dynamic flow energy, aeration, and permeability of the chitosan-mannitol binary carriers (CMBCs). CMBCs were self-designed as an advanced carrier with controllable surface roughness to obtain enhanced aerosolization performance. The specific mechanism of CMBCs to enhance the aerosolization performance of DPIs was elaborated based on the theory of pulmonary delivery processes by introducing powder properties. The results exhibited that CMBCs with appropriate surface roughness had lower special energy, lower aeration energy, and higher permeability. It could be predicted that CMBC-based DPIs had greater tendency to fluidize and disperse in airflow, and the lower adhesion force between particles enabled drugs to be detached from the carrier to achieve higher fine particle fractions. The specific mechanism on how physicochemical properties influenced the aerosolization performance during the pulmonary delivery processes could be figured out with the introduction of powder properties.
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http://dx.doi.org/10.1208/s12249-022-02287-9DOI Listing
June 2022

Guanidinium-rich lipopeptide functionalized bacteria-absorbing sponge as an effective trap-and-kill system for the elimination of focal bacterial infection.

Acta Biomater 2022 08 6;148:106-118. Epub 2022 Jun 6.

College of Pharmacy, Jinan University, Guangzhou 511443, China. Electronic address:

Focal bacterial infections are often difficult to treat due to the rapid emergence of antibiotic-resistant bacteria, high risk of relapse, and severe inflammation at local lesions. To address multidrug-resistant skin and soft tissue infections, a bacteria-absorbing sponge was prepared to involve a "trap-and-kill" mechanism. The system describes a guanidinium-rich lipopeptide functionalized lyotropic liquid-crystalline hydrogel with bicontinuous cubic networks. Amphiphilic lipopeptides can be spontaneously anchored to the lipid-water interface, exposing their bacterial targeting sequences to enhance antibacterial trapping/killing activity. Computational simulations supported our structural predictions, and the sponge was confirmed to successfully remove ∼98.8% of the bacteria in the medium. Release and degradation behavior studies indicated that the bacteria-absorbing sponge could degrade, mediate enzyme-responsive lipopeptide release, or generate ∼200 nm lipopeptide nanoparticles with environmental erosion. This implies that the sponge can effectively capture and isolate high concentrations of bacteria at the infected site and then sustainably release antimicrobial lipopeptides into deep tissues for the eradication of residual bacteria. In the animal experiment, we found that the antibacterial performance of the bacterial-absorbing sponge was significant, which demonstrated not only a long-term inhibition effect to disinfect and avoid bacterial rebound, but also a unique advantage to protect tissue from bacterial attack. STATEMENT OF SIGNIFICANCE: Host defense peptides/peptidomimetics (HDPs) have shown potential for the elimination of focal bacterial infections, but the application of their topical formulations suffers from time-consuming preparation processes, indistinctive toxicity reduction effects, and inefficient bacterial capture ability. To explore new avenues for the development of easily prepared, low-toxicity and high-efficiency topical antimicrobials, a guanidinium-rich lipopeptide was encapsulated in a lyotropic liquid-crystalline hydrogel (denoted as "bacteria-absorbing sponge") to achieve complementary superiorities. The superior characteristic of the bacteria-absorbing sponge involves a "trap-and-kill" mechanism, which undergoes not only a long-term inhibition effect to disinfect and avoid bacterial rebound, but also effective bacterial capture and isolating action to confine bacterial diffusion and protect tissues from bacterial attack.
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http://dx.doi.org/10.1016/j.actbio.2022.05.052DOI Listing
August 2022

Adsorption behavior of Cr(VI) by biomass-based adsorbent functionalized with deep eutectic solvents (DESs).

BMC Chem 2022 Jun 2;16(1):41. Epub 2022 Jun 2.

Department of Chemistry & Life Science, Hubei University of Education, Wuhan, 430205, China.

This study was aimed to evaluate the performance of DESs functionalized peanut shell (PSD) as biosorbent for removing Cr(VI) from water. The effects of pretreatment, initial concentration, adsorption temperature, kinetics, adsorption isotherm, and thermodynamics were investigated. Scanning electron microscopy (SEM) and Point of Zero charge (pHpzc) techniques were used for characterization of the adsorbents. The results showed that the rigid structure of peanut shell was broken down after DESs modification and the point of zero charge was 6.02 for peanut shell and 6.84 for PSD, which exhibited a slightly acid character. Based on the comparisons of linear and nonlinear analysis of four kinetic models and four isotherms, the pseudo-second-order kinetic model was found to be suitable for describing the adsorption process. The presence of a boundary effect was observed within the range of research, indicating that internal diffusion was not the only rate-controlling step. The equilibrium data were well represented by the Langmuir model rather than the Freundlich, Temkin, and Dubinin-Radushkevich models. The maximum capacity derived was 5.36 mg g. Changes in Gibb's free energy, enthalpy, and entropy revealed that Cr(VI) adsorption onto modified peanut-shell powders was a spontaneous and endothermic process. However, the highest desorption efficiency was only 8.77% by using NaOH as a desorbing agent.
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http://dx.doi.org/10.1186/s13065-022-00834-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161602PMC
June 2022

Author Correction: Copy number variation in the CES1 gene and the risk of non-alcoholic fatty liver in a Chinese Han population.

Sci Rep 2022 Jun 2;12(1):9242. Epub 2022 Jun 2.

Department of Epidemiology and Health Statistics, Fujian Provincial Key Laboratory of Environment Factors and Cancer, School of Public Health, Fujian Medical University, Fujian, 350122, China.

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http://dx.doi.org/10.1038/s41598-022-14015-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163088PMC
June 2022

Visible-NIR hyperspectral classification of grass based on multivariate smooth mapping and extreme active learning approach.

Sci Rep 2022 05 30;12(1):9017. Epub 2022 May 30.

College of Water Conservancy and Civil Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.

Grass community classification is the basis for the development of animal husbandry and dynamic monitoring of environment, which has become a critical problem to further strengthen the intelligent management of grassland. Compared with grass survey based on satellite remote sensing, the visible near infrared (NIR) hyperspectral not only monitor dynamically in a short distance, but also have high dimensions and detailed spectral information in each pixel. However, the hyperspectral labeled sample for classification is expensive and manual selection is more subjective. In order to solve above limitations, we proposed a visible-NIR hyperspectral classification model for grass based on multivariate smooth mapping and extreme active learning (MSM-EAL). Firstly, MSM is used to preprocess and reconstruct the spectrum. Secondly, by jointing XGBoost and active learning (AL), the advanced samples with the largest amount of information are actively selected to improve the performance of target classification. Innovation lies in: (1) MSM global enhanced preprocessing spectral reconstruction algorithm is proposed, in which isometric feature mapping is effectively applied to the grass hyperspectral for the first time. (2) EAL framework is constructed to solve the issue of high cost and small number for hyperspectral labeled samples, at the same time, enhance the physical essence behind spectral classification more intuitively. A field hyperspectral collection platform is assembled to establish nm resolution visible-NIR hyperspectral dataset of grass, Grass1, containing 750 samples, which to verify the effectiveness of the model. Experiments on the Grass1 dataset confirmed that compared with the full spectrum, the time consumption of MSM was reduced by 9.471 s with guaranteed overall accuracy (OA). Comparing EAL with AL, and other classification algorithms, EAL improves OA 22.2% over AL, and XAL has the best performance value on Kappa, Macro, Recall and F1-score, respectively. Altogether, the lightweight MSM-EAL model realizes intelligent and real-time classification, providing a new method for obtaining high-precision inter group classification of grass.
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http://dx.doi.org/10.1038/s41598-022-13136-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151682PMC
May 2022

Implantation of right ventricular endocardial pacing lead via paravalvular leak and subsequent paravalvular leak closure in a patient with mechanical tricuspid valve.

HeartRhythm Case Rep 2022 May 3;8(5):362-365. Epub 2022 Mar 3.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

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http://dx.doi.org/10.1016/j.hrcr.2022.02.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123329PMC
May 2022

Comparative Meta-Analysis of the Effects of OLIF and TLIF in Lumbar Spondylolisthesis Central Nerve Injury.

Comput Intell Neurosci 2022 10;2022:6861749. Epub 2022 May 10.

Qilu Hospital of Shandong University, Jinan 250000, Shandong, China.

Objective: The main objective is to explore the efficacy of oblique anterior lumbar fusion (OLIF) and transforaminal lumbar fusion (TLIF) in the treatment of lumbar spondylolisthesis central nerve injury.

Methods: The perioperative indexes, pain score (VAS), Oswestry dysfunction index (ODI), vertebral slip degree, slip angle, intervertebral space height, and quality of life score of the two groups were compared by meta-analysis.

Results: According to the observation indexes, the perioperative indexes of patients in the OLIF group were better than those in the TLIF group, which showed that the effect of OLIF treatment was better than of TLIF. The pain score and ODI score of the two groups can be obtained. The one-week postoperative pain degree and ODI of patients in the OLIF group are lower than those in the TLIF group, indicating that OLIF treatment will reduce the pain of patients to a greater extent and is more conducive to the recovery of patients. There was no significant difference in vertebral slip, slip angle, and intervertebral space height between the OLIF group and TLIF group. After treatment, the quality-of-life scores of patients in the OLIF group were significantly higher than those in the TLIF group.

Conclusion: The treatment of lumbar fusion through OLIF has irreplaceable perioperative advantages of TLIF, such as less bleeding, shorter operation time, less drainage and shorter hospital stay, less postoperative complications, less surgical wound, indirect decompression, no destruction of lumbar posterior stable structure, and maximum preservation of tissue structure. It has the advantages of reducing the intraoperative dural sac injury and nerve root traction injury and shortening the rehabilitation time of patients. It has the prospect of clinical application and can be popularized.
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http://dx.doi.org/10.1155/2022/6861749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113908PMC
May 2022

Determination and environmental risk assessment of organophosphorus flame retardants in sediments of the South China Sea.

Environ Sci Pollut Res Int 2022 May 19. Epub 2022 May 19.

College of Environmental Science and Engineering, Qingdao University, Qingdao, 266071, China.

As ubiquitous contaminants in the environment, organophosphorus flame retardants (OPFRs) would eventually settle in marine sediment. In this study, concentrations, spatial distributions, and ecological risks of seven OPFRs in sediment samples of the South China Sea (SCS) were investigated for the first time. Total concentration of all OPFRs ranged from 2.5 to 32.3 ng/g dry weight (dw), in which the abundance of tri-cresyl phosphates (TCPs) was the highest. OPFRs in the SCS were at a medium level compared with those from other parts of the world. The nearshore ocean current, ship transportation, and riverine inputs might influence the spatial distributions of OPFRs. The total inventory of six OPFRs in sediment was estimated to be 202.8 tons (16.7×10 km). The hazard quotient (HQ) of OPFRs ranged from 0 to 3.2E-02, indicating the ignorable ecological risk of OPFRs in sediments of the SCS. This study provides insight into the occurrence of current-use OPFRs in the SCS which deserved long-term concern in the future due to their continuous terrigenous inputs.
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http://dx.doi.org/10.1007/s11356-022-20752-7DOI Listing
May 2022

Inhalable Biomimetic Protein Corona-Mediated Nanoreactor for Self-Amplified Lung Adenocarcinoma Ferroptosis Therapy.

ACS Nano 2022 May 15. Epub 2022 May 15.

School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, Guangdong, China.

Ferroptosis therapy by catalyzing the Fenton reaction has emerged as a promising tumor elimination strategy for lung adenocarcinoma (ADC). However, the unsatisfactory Fenton reaction efficiency, strong intracellular antioxidant system, and insufficient lung drug accumulation limits the ferroptosis therapeutic effect. To address these issues, an inhalable nanoreactor was proposed by spontaneously adsorbing biomimetic protein corona (PC) composed of matrix metalloproteinase 2 responsive gelatin and glutamate (Glu) on the surface of cationic nanostructured lipid carriers (NLC) core loaded with ferrocene (Fc) and fluvastatin. The prepared Fc-NLC(F)@PC could be nebulized into lung lesions with 2.6 times higher drug accumulation and boost lipid peroxide production by 3.2 times to enhance ferroptosis therapy. Mechanically, fluvastatin was proved to inhibit monocarboxylic acid transporter 4 mediated lactate efflux, inducing tumor acidosis to boost Fc-catalyzing reactive oxygen species production, while the extracellular elevating Glu concentration was found to inhibit xCT (system X) functions and further collapse the tumor antioxidant system by glutathione synthesis suppression. Mitochondrial dysfunction and cell membrane damage were involved in the nanoreactor-driven ferroptotic cell death process. The enhanced antitumor effects by combination of tumor acidosis and antioxidant system collapse were confirmed in an orthotopic lung ADC tumor model. Overall, the proposed nanoreactor highlights the pulmonary delivery approach for local lung ADC treatment and underscores the great potential of ferroptosis therapy.
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http://dx.doi.org/10.1021/acsnano.2c02634DOI Listing
May 2022

Structural Origin of Enhanced Circularly Polarized Luminescence in Hybrid Manganese Bromides.

Angew Chem Int Ed Engl 2022 Jul 25;61(30):e202205906. Epub 2022 May 25.

Department of Chemistry, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.

Chiral hybrid metal halides with a high dissymmetry factor (g ) and a superior photoluminescence quantum yield (PLQY) are promising candidates for circularly polarized luminescence (CPL) light sources. Here, we report eight new chiral hybrid manganese halides, crystallizing in the non-centrosymmetric space group P2 2 2 and showing intense CPL emissions. Oppositely-signed circular dichroism (CD) and CPL signals are detected according to the R- and S-configurations of the chiral alkanolammonium cations. Time-resolved PL spectra show long averaged decay lifetimes up to 1 ms for (R-3-quinuclidinol)MnBr (R-1). The g of polycrystalline samples for coordinated structures (23×10 ) is more than doubled compared with the non-coordinated ones (8.5×10 ), due to the structural variations. R-1 exhibit both a high g and a high PLQY (50.2 %). The effective chirality transfer mechanism through coordination bonds, with strongly emissive Mn centers, enables a new class of high-performance CPL materials.
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http://dx.doi.org/10.1002/anie.202205906DOI Listing
July 2022

Isoliquiritigenin Ameliorates Ischemia-Induced Myocardial Injury via Modulating the Nrf2/HO-1 Pathway in Mice.

Drug Des Devel Ther 2022 29;16:1273-1287. Epub 2022 Apr 29.

Department of Cardiology, the Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, 225001, People's Republic of China.

Background: Oxidative stress and inflammatory reaction play critical roles in acute myocardial infarction (AMI). Isoliquiritigenin (ISL), a flavonoid monomer extracted from licorice, has been found to have antioxidant and anti-inflammatory effects in cancer studies. Here, we tested the effect and underlying mechanisms of ISL on ischemia-induced myocardial injury in a mouse AMI model.

Methods: Adult C57BL/6 mice were pre-treated by intraperitoneal injection of ISL and/or a specific nuclear factor E2-related factor 2 (Nrf2) inhibitor ML385 for 3 days, respectively. Then, the AMI model was established by ligating the anterior descending branch of the left coronary artery. Myocardial oxidative stress status, inflammatory response, cardiac function and infarction size were assessed after 7th day of surgery.

Results: Compared with sham group, the reactive oxygen species (ROS) and malondialdehyde (MDA) level in AMI group were significantly increased. However, the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) level were dramatically decreased. ISL treatment significantly reduced the myocardial infarction area, improved cardiac function, inhibited the production of ROS and MDA and reduced the consumption of SOD and GSH-Px. Interestingly, ISL could significantly increase nuclear Nrf2 and cytosolic heme oxygenase 1 (HO-1) level in the infarcted myocardium and reduce the oxidative stress after AMI. Also, ISL treatment dramatically inhibited the activation of myocardial NF-κB pathway and reduced the expression of pro-inflammatory factors in the AMI group. However, the administration of ML385 not only suppressed the Nrf2/HO-1 activation, the anti-oxidant and anti-inflammatory effects induced by ISL, but also attenuated the beneficial role of ISL on reducing infarct size and improving cardiac function in the mouse with AMI.

Conclusion: The results suggested that activation of Nrf2/HO-1 pathway has an essential role in ISL-induced cardiac protection by alleviating myocardial oxidative stress and inflammation response in mice with AMI.
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http://dx.doi.org/10.2147/DDDT.S362754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064455PMC
May 2022

Improvement of antioxidant properties of jujube puree by biotransformation of polyphenols via fermentation.

Food Chem X 2022 Mar 17;13:100214. Epub 2022 Jan 17.

College of Food Science and Nutritional Engineering, China Agricultural University, National Engineering Research Center for Fruit & Vegetable Processing, Key Laboratory of Fruit & Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory for Food Non-thermal Processing, Beijing 100083, China.

To investigate the effect of lactic acid bacteria fermentation on jujube bioactivity, was used to ferment jujube puree. The number of viable bacteria cells, physicochemical properties, phenolics profile and antioxidant capacity were analyzed, and their correlation were investigated. exhibited a high growth capacity in jujube puree, and significantly ( < 0.05) increased the total phenolics content, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and reducing power after 48 h fermentation, while 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity was decreased. 12 differentially metabolized polyphenols were identified in fermented jujube puree. Upregulated phenolics exhibited a positive correlation with DPPH radical-scavenging ability and reducing power. This work demonstrated that fermentation can be an effective method with great practical application potential to improve the antioxidant activity in jujube puree by modifying the phenolic compositional quantity and quality.
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http://dx.doi.org/10.1016/j.fochx.2022.100214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039917PMC
March 2022

Non-covalent interactions of selected flavors with pea protein: Role of molecular structure of flavor compounds.

Food Chem 2022 Sep 22;389:133044. Epub 2022 Apr 22.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address:

The influence of the molecular structures of flavor compounds (specifically, variations in chain length and functional groups) on the binding of the flavor compounds (Z)-2-penten-1-ol, hexanal, and (E)-2-octenal to pea protein was investigated. The results showed that the molecular structures of the flavor compounds strongly influenced their binding affinity for pea protein. Specifically, (E)-2-octenal exhibited a higher binding affinity and a higher Stern-Volmer constant with pea protein than both hexanal and (Z)-2-penten-1-ol. Thermodynamic analysis indicated that the flavor compound-pea protein interactions were spontaneous. Hydrophobic interactions were dominant in the non-covalent interactions between (E)-2-octenal/(Z)-2-penten-1-ol and pea protein, whereas hydrogen bonding was dominant in the non-covalent interactions between hexanal and pea protein. Surface hydrophobicity measurements, the use of bond-disrupting agents, and molecular docking further supported the hypothesis that hydrogen bonding, as well as hydrophobic interactions, occurred between the flavor compounds and pea protein.
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http://dx.doi.org/10.1016/j.foodchem.2022.133044DOI Listing
September 2022

Pulmonary delivery nanomedicines towards circumventing physiological barriers: Strategies and characterization approaches.

Adv Drug Deliv Rev 2022 06 22;185:114309. Epub 2022 Apr 22.

School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, Guangdong, PR China. Electronic address:

Pulmonary delivery of nanomedicines is very promising in lung local disease treatments whereas several physiological barriers limit its application via the interaction with inhaled nanomedicines, namely bio-nano interactions. These bio-nano interactions may affect the pulmonary fate of nanomedicines and impede the distribution of nanomedicines in its targeted region, and subsequently undermine the therapeutic efficacy. Pulmonary diseases are under worse scenarios as the altered physiological barriers generally induce stronger bio-nano interactions. To mitigate the bio-nano interactions and regulate the pulmonary fate of nanomedicines, a number of manipulating strategies were established based on size control, surface modification, charge tuning and co-delivery of mucolytic agents. Visualized and non-visualized characterizations can be employed to validate the robustness of the proposed strategies. This review provides a guiding overview of the physiological barriers affecting the in vivo fate of inhaled nanomedicines, the manipulating strategies, and the validation methods, which will assist with the rational design and application of pulmonary nanomedicine.
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http://dx.doi.org/10.1016/j.addr.2022.114309DOI Listing
June 2022

Targeting impaired autophagy as a therapeutic strategy for Koolen-de Vries syndrome.

Autophagy 2022 Apr 29:1-3. Epub 2022 Apr 29.

Department of Cell Biology, State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, China Beijing, China.

Koolen-de Vries syndrome (KdVS) is a genomic disorder characterized by intellectual disability, heart failure, hypotonia and congenital malformations, which is caused by haploinsufficiency of . Because the pathogenesis of the disease is unknown, there is still no effective treatment. Here, we discuss our recent work identifying as an essential gene for macroautophagy/autophagy. We find that KANSL1 modulates autophagosome-lysosome fusion for cargo degradation by transcriptionally regulating expression. heterozygous mice exhibit impaired neuronal and cardiac functions, resulting from the obstruction of autophagic clearance of damaged mitochondria and accumulation of reactive oxygen species in these tissues. Furthermore, we discovered an FDA-approved drug, 13- retinoic acid, is capable of alleviating these mitophagic defects and neurobehavioral abnormalities in heterozygous mice by promoting autophagosome-lysosome fusion via directly binding to STX17 and SNAP29. Our study provides the proof of concept to set up a link between KANSL1, autophagic defects and KdVS, and also proposes a therapeutic strategy for treatment of KdVS.
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http://dx.doi.org/10.1080/15548627.2022.2069904DOI Listing
April 2022

Nur77 Deficiency Exacerbates Macrophage NLRP3 Inflammasome-Mediated Inflammation and Accelerates Atherosclerosis.

Oxid Med Cell Longev 2022 13;2022:2017815. Epub 2022 Apr 13.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China.

Purpose: Activation of NLR (nucleotide-binding and leucine-rich repeat immune receptor) family pyrin domain containing 3 (NLRP3) inflammasome mediating interleukin- (IL-) 1 secretion has emerged as an important component of inflammatory processes in atherogenesis. The nuclear receptor Nur77 is highly expressed in human atherosclerotic lesions; however, its functional role in macrophage NLRP3 inflammasome activation has not yet been clarified. . Eight-week-old apolipoprotein E (ApoE)-/- and ApoE-/- Nur77-/- mice that were fed a Western diet underwent partial ligation of the left common carotid artery (LCCA) and left renal artery (LRA) to induce atherogenesis. Four weeks later, severe plaque burden associated with increased lipid deposition, reduced smooth muscle cells, macrophage infiltration, and decreased collagen expression was identified in ApoE-/- Nur77-/- mice compared with those in ApoE-/- mice. ApoE-/- Nur77-/- mice showed increased macrophage inflammatory responses in carotid atherosclerotic lesions. In vitro studies demonstrated that oxidized low-density lipoprotein cholesterol (ox-LDL) increased the release of lactate dehydrogenase (LDH) and upregulated the expressions of cleaved caspase-1, cleaved IL-1 and gasdermin D (GSMD) in WT peritoneal macrophages (PMs) in a NLRP3-dependent manner. Nur77-/- PMs exhibited a further increased level of NLRP3 inflammasome-mediated inflammation under ox-LDL treatment compared with WT PMs. Mechanistically, Nur77 could bind to the promoter of NLRP3 and inhibit its transcriptional activity.

Conclusions: This study demonstrated that Nur77 deletion promotes atherogenesis by exacerbating NLRP3 inflammasome-mediated inflammation.
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http://dx.doi.org/10.1155/2022/2017815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020982PMC
April 2022

Low Drug Loading Hampers the Clinical Translation of Peptide Drugs-Containing Metered-Dose Inhalers.

Pharmaceuticals (Basel) 2022 Mar 23;15(4). Epub 2022 Mar 23.

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.

Peptide-based drugs have attracted extensive attention from the medical and pharmaceutical industry because of their relatively high safety and efficacy. However, most of the peptide drugs approved are administrated by injection, which can easily cause poor patient compliance. In this circumstance, pulmonary administration as an alternative to injection administration can not only avoid the above issue but also accelerate the absorption rate of peptide drugs and improve bioavailability. Among the pulmonary delivery systems available on the market, metered-dose inhalers (MDIs) have emerged as appealing candidates for pulmonary delivery systems with clinical translational value, owing to their many merits, including portable, easy-to-operate, and cost-effective properties. Nevertheless, the industrialization of peptide drugs-containing MDIs encounters a bottleneck of low drug loading, owing to the incompatibility between the propellant and the peptide drugs, which cannot be effectively overcome by the current carrier particle encapsulation strategy. Herein, we put forward the following strategies: (1) To screen amphiphilic materials with high surface activity and strong interaction with peptide drugs; (2) To construct a chemical connection between peptide drugs and amphiphilic substances; (3) To optimize the cosolvent for dispersing peptide drugs. We suppose these strategies have the potential to defeat the bottleneck problem and provide a new idea for the industrialization of peptide drugs-containing MDIs.
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http://dx.doi.org/10.3390/ph15040389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031202PMC
March 2022
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