Publications by authors named "Xin Lv"

295 Publications

CircPVT1 promotes proliferation of lung squamous cell carcinoma by binding to miR-30d/e.

J Exp Clin Cancer Res 2021 Jun 10;40(1):193. Epub 2021 Jun 10.

School of Medicine, Southern University of Science and Technology, 1088th Xueyuan Road, Shenzhen, 518055, People's Republic of China.

Background: Circular RNAs (circRNAs) are a new type of extensive non-coding RNAs that regulate the activation and progression of different human diseases, including cancer. However, information on the underlying mechanisms and clinical significance of circRNAs in lung squamous cell carcinoma (LUSC) remains scant.

Methods: The expression profile of RNAs in 8 LUSC tissues, and 9 healthy lung tissues were assayed using RNA sequencing (RNA-seq) techniques. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to profile the expression of circPVT1 and its relationship with the prognosis of LUSC, i.e., survival analysis. Moreover, in vitro and in vivo experiments were performed to evaluate the impacts of circPVT1 on the growth of tumors. RNA pull-down tests, mass spectrometry, dual-luciferase reporter assessment, and RNA immune-precipitation tests were further conducted to interrogate the cross-talk between circPVT1, HuR, or miR-30d/e in LUSC.

Results: Our data showed that circPVT1 was upregulated in LUSC tissues, serum, and cell lines. LUSC patients with higher circPVT1 expression exhibited shorter survival rates. The in vivo and in vitro data revealed that circPVT1 promotes the proliferation of LUSC cells. Additionally, mechanistic analysis showed that HuR regulated circPVT1. On the other hand, circPVT1 acted as a competing endogenous RNA (ceRNA) of miR-30d and miR-30e in alleviating the suppressive influences of miR-30d and miR-30e on its target cyclin F (CCNF).

Conclusion: CircPVT1 promotes LUSC progression via HuR/circPVT1/miR-30d and miR-30e/CCNF cascade. Also, it acts as a novel diagnostic biomarker or treatment target of individuals diagnosed with LUSC.
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http://dx.doi.org/10.1186/s13046-021-01976-wDOI Listing
June 2021

Folic acid alleviates age-related cognitive decline and inhibits apoptosis of neurocytes in senescence-accelerated mouse prone 8: deoxythymidine triphosphate biosynthesis as a potential mechanism.

J Nutr Biochem 2021 Jun 5:108796. Epub 2021 Jun 5.

Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin 300070, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin 300070, China.

Disturbed deoxythymidine triphosphate (dTTP) biosynthesis due to the inhibition of thymidylate synthase (TS) can lead to uracil accumulation in DNA, eventually, lead to neurocytes apoptosis and cognitive decline. Folic acid supplementation delayed cognitive decline and neurodegeneration in senescence-accelerated mouse prone 8 (SAMP8). Whether folic acid, one of nutrition factor, the effect on the expression of TS is unknown. The study aimed to determine if folic acid supplementation could alleviate age-related cognitive decline and apoptosis of neurocytes by increasing TS expression in SAMP8 mice. According to folic acid concentration in diet, four-month-old male SAMP8 mice were randomly divided into three different diet groups by baseline body weight in equal numbers. Moreover, to evaluate the role of TS, a TS inhibitor was injected intraperitoneal. Cognitive test, apoptosis rates of neurocytes, expression of TS, relative uracil level in telomere, and telomere length in brain tissue were detected. The results showed that folic acid supplementation decreased deoxyuridine monophosphate accumulation, uracil misincorporation in telomere, alleviated telomere length shorting, increased expression of TS, then decreased apoptosis rates of neurocytes, and alleviated cognitive performance in SAMP8 mice. Moreover, at the same concentration of folic acid, TS inhibitor raltitrexed increased deoxyuridine monophosphate accumulation, uracil misincorporation in telomere, and exacerbated telomere length shorting, decreased expression of TS, then increased apoptosis rates of neurocytes, and decreased cognitive performance in SAMP8 mice. In conclusion, folic acid supplementation alleviated age-related cognitive decline and inhibited apoptosis of neurocytes by increasing TS expression in SAMP8 mice.
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http://dx.doi.org/10.1016/j.jnutbio.2021.108796DOI Listing
June 2021

Neuroprotective Activities of Constituents from Phyllosticta capitalensis, an Endophyte Fungus of Loropetalum chinense var. rubrum.

Chem Biodivers 2021 Jun 8. Epub 2021 Jun 8.

China Pharmaceutical University, Department of Natural Medicinal Chemistry, China Pharmaceutical University Xuanwumen Campus, No.24, Tongjiaxiang, Gulou Dis, China Pharmaceutical University Jiangning Campus, No.639,Longmiandadao, Jiangnin, 210009, Nanjing, CHINA.

One new dioxolanone derivative, guignardianone G ( 1 ) and twelve known compounds ( 2 - 13 ) were isolated from the 95% ethanol extract of the plant endophytic fungus Phyllosticta capitalensis cultured in rice medium. Among the known compounds isoaltenuene ( 3 ), brassicasterol ( 7 ), 5,6-epoxyergosterol ( 8 ), citreoanthrasteroid A ( 9 ), demethylincisterol A ( 10 ), chaxine C ( 11 ) were reported from Phyllosticta sp. for the first time. The structures of 1 was elucidated by 1D- and 2D-NMR experiments and HR-ESI-MS data analysis, and its absolute configuration was established through the comprehensive use of the methods of modified Mosher methods, calculations of ECD spectra and optical rotation values. The neuroprotective activity of compounds 1 - 9 , 11 - 13 were evaluated on PC12 cells damage induced by glutamate, and compounds 9 and 12 showed potential neuroprotective activities and compounds 9 and 12 with half effective concentration (EC 50 ) of 24.2 and 33.9 μ M, respectively.
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http://dx.doi.org/10.1002/cbdv.202100314DOI Listing
June 2021

Global Trends in Research of Macrophages Associated With Acute Lung Injury Over Past 10 Years: A Bibliometric Analysis.

Front Immunol 2021 20;12:669539. Epub 2021 May 20.

Department of Anesthesiology, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China.

Acute lung injury (ALI) is an intractable disorder associated with macrophages. This bibliometric analysis was applied to identify the characteristics of global scientific output, the hotspots, and frontiers about macrophages in ALI over the past 10 years. We retrieved publications published from 2011 to 2020 and their recorded information from Science Citation Index Expanded (SCI-expanded) of Web of Science Core Collection (WoSCC). Bibliometrix package was used to analyze bibliometric indicators, and the VOSviewer was used to visualize the trend and hotspots of researches on macrophages in ALI. Altogether, 2,632 original articles were reviewed, and the results showed that the annual number of publications (Np) concerning the role of macrophages in ALI kept increasing over the past 10 years. China produced the most papers, the number of citations (Nc) and H-index of the USA ranked first. Shanghai Jiaotong University and INT IMMUNOPHARMACOL were the most prolific affiliation and journal, respectively. Papers published by Matute-Bello G in 2011 had the highest local citation score (LCS). Recently, the keywords "NLRP3" and "extracellular vesicles" appeared most frequently. Besides, researches on COVID-19-induced ALI related to macrophages seemed to be the hotspot recently. This bibliometric study revealed that publications related to macrophages in ALI tend to increase continuously. China was a big producer and the USA was an influential country in this field. Most studies were mainly centered on basic researches in the past decade, and pathways associated with the regulatory role of macrophages in inhibiting and attenuating ALI have become the focus of attention in more recent studies. What is more, our bibliometric analysis showed that macrophages play an important role in COVID-19-induced ALI and may be a target for the treatment of COVID-19.
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http://dx.doi.org/10.3389/fimmu.2021.669539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173163PMC
June 2021

Are oil spills enhancing outbreaks of red tides in the Chinese coastal waters from 1973 to 2017?

Environ Sci Pollut Res Int 2021 May 31. Epub 2021 May 31.

Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, People's Republic of China.

Between 1973 and 2017, evidences of red tide outbreaks and oil spill accidents in the Chinese coastal waters were collected. Statistical analysis and multiple regression models were used to determine the relationship between the red tide and the oil spill. Major findings reveal that (1) the frequency of red tides positively correlates to the number of oil spills and the volume of oil spilled as well; (2) the higher percentage of small spills (< 7 tonnes) are more likely to enhance the outbreaks of red tides; (3) both EI Niño and storm events do not show any relationship with red tides; and (4) more severe oil spill with penalty recorded implies a higher possibility to trigger the red tide afterwards. Therefore, oil spill contingency management focusing on small oil spills and mitigating their spill effect by physical measures could be of benefit to decrease the frequency of red tides significantly. For example, it is suggested to carry out physical combat instead of chemical dispersants to remove the spilled small oil in the shallow coastal areas for reducing the outbreak risk of red tides after the oil spill.
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http://dx.doi.org/10.1007/s11356-021-14549-3DOI Listing
May 2021

Role of Ferroptosis in Lung Diseases.

J Inflamm Res 2021 20;14:2079-2090. Epub 2021 May 20.

Department of Anesthesiology, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui, 236000, People's Republic of China.

Ferroptosis is a new type of programmed cell death characterized by intracellular iron accumulation and lipid peroxidation that leads to oxidative stress and cell death. The metabolism of iron, lipids, and amino acids and multiple signalling pathways precisely regulate the process of ferroptosis. Emerging evidence has demonstrated that ferroptosis participates in the occurrence and progression of various pathological conditions and diseases, such as infections, neurodegeneration, tissue ischaemia-reperfusion injury and immune diseases. Recent studies have also indicated that ferroptosis plays a critical role in the pathogenesis of acute lung injury, chronic obstructive pulmonary disease, pulmonary fibrosis, pulmonary infection and asthma. Herein, we summarize the latest knowledge on the regulatory mechanism of ferroptosis and its association with iron, lipid and amino acid metabolism as well as several signalling pathways. Furthermore, we review the contribution of ferroptosis to the pathogenesis of lung diseases and discuss ferroptosis as a novel therapeutic target for various lung diseases.
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http://dx.doi.org/10.2147/JIR.S307081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144020PMC
May 2021

A Retrospective Cohort Study on the Clinical Course of Patients With Moderate-Type COVID-19.

Front Public Health 2021 26;9:593109. Epub 2021 Apr 26.

Department of Pulmonary and Critical Care Medicine, The Third Xiangya Hospital of Central South University, Changsha, China.

A large number of people contracted moderate-type COVID-19 around the world. However, to our knowledge no studies have covered the clinical course of patients with moderate-type COVID-19. This study describes the clinical course of moderate-type patients with COVID-19 from Wuhan City and Yiyang City, and explores factors relevant to the length of hospitalization and symptoms relief. The study analyzed the clinical course of 107 moderate-type patients with COVID-19 from the outbreak area (Wuhan) and the imported area (Yiyang), and used automatic linear modeling and multivariate linear regression analysis to explore the factors relevant to the length of hospitalization and symptoms relief. Furthermore, we created a scoring system to value the length of hospitalization and symptoms relief. Lymphopenia, elevated C-reactive protein, increased LDH, bilateral lung GGO (ground glass opacity), and lung consolidation were more likely to appear in ordinary inpatients with moderate-type COVID-19 from Wuhan ( < 0.05), compared to infected medical staff from Wuhan and ordinary inpatients with moderate-type COVID-19 from Yiyang. Meanwhile, the length of hospitalization and symptoms relief was longer in ordinary patients with moderate-type COVID-19 from Wuhan ( < 0.05). Onset of symptoms to admission, ESR, leucocytes count, and bilateral lung GGO were linearly related to the length of hospitalization ( < 0.05); onset of symptoms to admission, leucocytes count, bilateral lung GGO, and lung consolidation were linearly related to the length of symptoms relief ( < 0.05). By using the scoring system, we found that the time of hospitalization and symptoms relief lengthened as the scores increased. This study described the clinical course of patients with moderate-type COVID-19, and found that ordinary patients with moderate-type COVID-19 in outbreak areas were more serious and needed stronger treatment and longer treatment time. Onset of symptoms to admission, ESR, leucocytes count, and bilateral lung GGO can be effective predictors of the length of hospitalization. And onset of symptoms to admission, leucocytes count, bilateral lung GGO, and lung consolidation can be effective predictors of the amount of time until symptoms relief. Most importantly, we have created a scoring system, which could contribute to the diagnosis and treatment of COVID-19.
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http://dx.doi.org/10.3389/fpubh.2021.593109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112071PMC
May 2021

Neuroprotective effect of stroke pretreated MSCs against cerebral ischemia/reperfusion injury in rats.

World Neurosurg 2021 May 3. Epub 2021 May 3.

Department of General Medicine, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, China. Electronic address:

Background: Mesenchymal stem cells (MSCs) have been shown to enhance neurological recovery after stroke. In this study, a middle cerebral artery occlusion (MCAO) rat model was designed to assess the neuroprotective effects of stroke pretreated MSCs on cerebral ischemia/reperfusion (I/R) injury.

Method: The MSCs were isolated and cultured in medium with 10% FBS, normal control serum (NS), or stroke serum (SS). Then these MSCs were injected into each group rats (n=6) 1 day after MCAO and continue feeding for 28 days. A battery of behavioral tests, TTC staining, hematoxylin and eosin staining, ELISA, and TUNEL assay were used to assess neural injury. Moreover, in order to detected the enhancement of neuronal regeneration and angiogenesis, immunofluorescence and western blotting were performed to assess the expression of trophic factor and growth factor.

Result: After treatment, the behaviour was improvement significantly. And the infarct area, brain lesion, and apoptosis cells were significantly decreased in the SS-MSCs group than others. It also modulated the inflammation by attenuating inflammatory cytokines. In addition, the number of neurogenesis positive cells, the expression of trophic factors and growth factors were significantly higher in the SS-MSCs group than others. At the same time, FBS-MSCs and NS-MSCs showed differences in the expression of trophic factors and growth factors, but the results were not as well as SS-MSCs.

Conclusion: SS-MSCs administration after the reperfusion led to neuroprotection by improving pathological changes, behavioral improvement, neurogenesis, suppression of apoptosis and inflammatory as well as angiogenesis.
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http://dx.doi.org/10.1016/j.wneu.2021.04.114DOI Listing
May 2021

Extracellular histones aggravate inflammation in ARDS by promoting alveolar macrophage pyroptosis.

Mol Immunol 2021 Jul 16;135:53-61. Epub 2021 Apr 16.

Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200433, PR China. Electronic address:

Extracellular histones have been discovered to play a pathogenic role in ARDS, but the underlying mechanisms are yet to be fully defined. Alveolar macrophage (AM) is essential for the initiation and progression of lung inflammation; of note, AM pyroptosis has been suggested contributing to ARDS-associated inflammation. Here we aimed to investigate whether extracellular histones promote ARDS by triggering AM pyroptosis. The BALF samples of ARDS patients were collected and AMs were further isolated. Extracellular histones, AM pyroptosis, and pyroptosis-associated mediators were measured. Furthermore, the effects of extracellular histones on AM pyroptosis and the underlying mechanisms were investigated. It showed that extracellular histones were markedly elevated in the BALF of ARDS patients and correlated with the increased AM pyroptosis. ARDS patient's BALF induced pronounced pyroptosis in cultured human monocytes, which could be prevented by neutralizing extracellular histones with heparin. In addition, exogenous histones induced pyroptosis of MH-S cells in a dose- and time-dependent manner, which acted through the NLRP3 inflammasome signaling pathway. Inhibition of NLRP3 inflammasome signaling substantially reduced cell pyroptosis. In a murine model of LPS-induced ARDS, extracellular histones were increased in the BALF and its increase was associated with enhanced AM pyroptosis and exaggerated lung inflammation. Blockade of extracellular histones or NLPR3 inflammasome equally inhibited macrophage pyroptosis, whereas targeting histones appeared more effective in alleviating lung inflammation. This study suggested that extracellular histones promote AM pyroptosis through NLRP3 inflammasome pathway, which in turn aggravates lung inflammation in ARDS. Pharmacological manipulation of extracellular histones or AM pyroptosis may become promising strategies for the treatment of ARDS.
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http://dx.doi.org/10.1016/j.molimm.2021.04.002DOI Listing
July 2021

Water flow and temperature drove epiphytic microbial community shift: Insight into nutrient removal in constructed wetlands from microbial assemblage and co-occurrence patterns.

Bioresour Technol 2021 Jul 7;332:125134. Epub 2021 Apr 7.

Yellow River Institute of Hydraulic Research, Zhengzhou 450003, China.

The impacts of water flow and low temperature on nutrient removal and underlying ecological mechanism of epiphytic microbial community in constructed wetlands remain to be fully illustrated. In this study, low temperature inhibited the decrease of TN, NH-N, TP, and COD concentrations in water, but water flow decreased NH-N and COD concentrations strikingly. The relative conductivity, soluble sugar, and protein of M. spicatum increased, while the total chlorophyll contents decreased significantly under the stress of water flow and low temperature. Temperature affected the alpha-diversity and composition of the microbial community, while water flow caused differences in community distribution. Deterministic processes dominated in microbial community assembly with increasing environmental stress. Co-occurrence network analysis demonstrated that Chlorophyta, Verrucomicrobia, Proteobacteria, Bacteroidetes, and Firmicutes phyla were the dominant hubs in September, however, low temperatures caused a shift to Metazoan dominated network, demonstrating diminished nutrient removal capacity.
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http://dx.doi.org/10.1016/j.biortech.2021.125134DOI Listing
July 2021

Ultrasound-Guided Continuous Thoracic Paravertebral Infusion of Methylene Blue in the Treatment of Postherpetic Neuralgia: A Prospective, Randomized, Controlled Study.

Pain Ther 2021 Jun 11;10(1):675-689. Epub 2021 Apr 11.

Department of Anesthesiology and Critical Care Medicine, Shanghai East Hospital, Tongji University School of Medicine, 150 Jimo Rd, Shanghai, 200120, China.

Introduction: Postherpetic neuralgia (PHN) is the most common complication of herpes zoster. Methylene blue (MB) is an inhibitor of nitric oxide synthesis with potentially analgesic and anti-inflammatory properties. Studies have demonstrated that thoracic paravertebral single MB injection is effective in treating chronic pain. However, there are rare reports of the efficacy of continuous thoracic paravertebral infusion of MB for pain management in PHN patients. The purpose of this study was to evaluate the therapeutic effects of continuous thoracic paravertebral infusion of MB on PHN.

Methods: A total of 104 PHN patients were randomly divided into two groups: the control group (continuous thoracic paravertebral infusion of 5% lidocaine in a total volume of 300 ml) and the MB group (continuous thoracic paravertebral infusion of 5% lidocaine plus 0.2% MB in a total volume of 300 ml). All patients were evaluated using the Numerical Rating Scale (NRS), Insomnia Severity Index (ISI), Patient Health Questionnaire-9 (PHQ-9), 36-Item Short-Form Health Survey (SF-36), and medication doses before and after the procedure. The effective treatment rate and adverse complications were recorded 6 months after the procedure.

Results: In both groups, the NRS scores, ISI scores, PHQ-9 scores, and rescue medication dosages were significantly decreased at different time points after treatment compared to baseline, while the SF-36 scores were evidently improved at different time points after treatment compared to baseline. Compared with the control group, the MB group had significantly reduced NRS scores, ISI scores, PHQ-9 scores, and rescue medication dosages at each observation time point. Furthermore, the SF-36 scores in the MB group were significantly higher than those in the control group at each observation time point. The total effective treatment rate of the MB group was higher than that of the control group 6 months after the procedure. No severe adverse complications were observed in either group.

Conclusions: Ultrasound-guided continuous thoracic paravertebral infusion with MB is a safe and effective therapy for PHN. Continuous infusion with MB can significantly reduce pain intensity, improve pain-related depression, increase quality of life, and decrease the amount of rescue medicine with no serious adverse complications.
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http://dx.doi.org/10.1007/s40122-021-00265-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119597PMC
June 2021

Clinical importance of the distribution of pulmonary artery embolism in acute pulmonary embolism.

J Int Med Res 2021 Apr;49(4):3000605211004769

Department of Geriatrics, Linyi People's Hospital, Linyi, China.

Objective: To explore the clinical importance of the distribution of pulmonary artery embolism in acute pulmonary embolism (APE).

Methods: Sixty-four patients with APE were classified into mixed-type and distal-type pulmonary embolism groups. Their right ventricular systolic pressure (RVSP) and disease duration were recorded, and the diameter of their right ventricles was measured by ultrasound. The computed tomography angiographic clot load was determined as a Mastora score.

Results: Patients with distal-type pulmonary embolisms had significantly lower RVSPs (44.92 ± 17.04 vs 55.69 ± 17.66 mmHg), and significantly smaller right ventricular diameters (21.08 ± 3.06 vs 23.37 ± 3.48 mm) than those with mixed-type pulmonary embolisms. Additionally, disease duration was significantly longer in patients with distal-type pulmonary embolisms (14.33 ± 11.57 vs 8.10 ± 7.10 days), and they had significantly lower Mastora scores (20.91% ± 18.92% vs 43.96% ± 18.30%) than patients with mixed-type pulmonary embolisms. After treatment, RVSPs decreased significantly in patients with both distal-type and mixed-type pulmonary embolisms. Right ventricle diameters also decreased significantly in patients with mixed-type pulmonary embolisms after treatment.

Conclusion: Patients with mixed-type pulmonary embolisms are significantly more susceptible to pulmonary hypertension, enlarged right ventricular diameters, and shorter durations of disease than those with distal-type pulmonary embolisms. The distribution of pulmonary artery embolism in APE can provide a clinical reference.
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http://dx.doi.org/10.1177/03000605211004769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033481PMC
April 2021

Progressive Study on the Non-thermal Effects of Magnetic Field Therapy in Oncology.

Front Oncol 2021 17;11:638146. Epub 2021 Mar 17.

College of Instrumentation and Electrical Engineering, Jilin University, Changchun, China.

Cancer is one of the most common causes of death worldwide. Although the existing therapies have made great progress and significantly improved the prognosis of patients, it is undeniable that these treatment measures still cause some serious side effects. In this context, a new treatment method is needed to address these shortcomings. In recent years, the magnetic fields have been proposed as a novel treatment method with the advantages of less side effects, high efficiency, wide applications, and low costs without forming scars. Previous studies reported that static magnetic fields (SMFs) and low-frequency magnetic fields (LF-MFs, frequency below 300 Hz) exert anti-tumor function, independent of thermal effects. Magnetic fields (MFs) could inhibit cell growth and proliferation; induce cell cycle arrest, apoptosis, autophagy, and differentiation; regulate the immune system; and suppress angiogenesis and metastasis various signaling pathways. In addition, they are effective in combination therapies: MFs not only promote the absorption of chemotherapy drugs by producing small holes on the surface of cell membrane but also enhance the inhibitory effects by regulating apoptosis and cell cycle related proteins. At present, MFs can be used as drug delivery systems to target magnetic nanoparticles (MNPs) to tumors. This review aims to summarize and analyze the current knowledge of the pre-clinical studies of anti-tumor effects and their underlying mechanisms and discuss the prospects of the application of MF therapy in cancer prevention and treatment.
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http://dx.doi.org/10.3389/fonc.2021.638146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010190PMC
March 2021

Methods of Lipidomic Analysis: Extraction, Derivatization, Separation, and Identification of Lipids.

Adv Exp Med Biol 2021 ;1280:173-187

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, People's Republic of China.

Lipidomics refers to the large-scale study of pathways and networks of cellular lipids in biological systems. A lipidomic analysis often involves the identification and quantification of the thousands of cellular lipid molecular species within a complex biological sample and therefore requires a well optimized method for lipid profiling. In this chapter, the methods for lipidomic analysis, including sample collection and preparation, lipid derivatization and separation, mass spectrometric identification of lipids, data processing and interpretation, and quality control, are overviewed.
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http://dx.doi.org/10.1007/978-3-030-51652-9_12DOI Listing
April 2021

Active destruction of pyrite passivation by ozone oxidation of a biotic leaching system.

Chemosphere 2021 Aug 19;277:130335. Epub 2021 Mar 19.

School of Minerals Processing & Bioengineering, Central South University, Changsha, Hunan, China; Key Lab of Biohydrometallurgy of Ministry of Education, Changsha, Hunan, China.

Although pyrite bio-dissolution plays an important role in the processing of sulfide ores, the formation of passivation film inhibited the further dissolution of sulfide ores. In order to enhance the dissolution of sulfide ores, a novel method for destroying the passivation film using ozone was proposed and verified. The generated passivation film inhibiting pyrite dissolution in the presence of Leptospirillum ferrooxidans and Acidithiobacillus thiooxidans was studied. The X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) results indicate that a passivation film mainly consisting of jarosite and polysulfide (S/S) might be formed during biotic stage, which can be eliminated with the introduction of ozone (2 g/min) in 30 min. Electrochemical results show that ozone significantly increased the electrochemical reactivity of passivated pyrite, further proving that ozone enhanced the dissolution of passivated pyrite through destroying the passivation layer. Hence, a bi-stage method for dissolution of sulfide ores can be proposed.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130335DOI Listing
August 2021

Abnormal upregulation of cardiovascular disease biomarker PLA2G7 induced by proinflammatory macrophages in COVID-19 patients.

Sci Rep 2021 03 24;11(1):6811. Epub 2021 Mar 24.

NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.

High rate of cardiovascular disease (CVD) has been reported among patients with coronavirus disease 2019 (COVID-19). Importantly, CVD, as one of the comorbidities, could also increase the risks of the severity of COVID-19. Here we identified phospholipase A2 group VII (PLA2G7), a well-studied CVD biomarker, as a hub gene in COVID-19 though an integrated hypothesis-free genomic analysis on nasal swabs (n = 486) from patients with COVID-19. PLA2G7 was further found to be predominantly expressed by proinflammatory macrophages in lungs emerging with progression of COVID-19. In the validation stage, RNA level of PLA2G7 was identified in nasal swabs from both COVID-19 and pneumonia patients, other than health individuals. The positive rate of PLA2G7 were correlated with not only viral loads but also severity of pneumonia in non-COVID-19 patients. Serum protein levels of PLA2G7 were found to be elevated and beyond the normal limit in COVID-19 patients, especially among those re-positive patients. We identified and validated PLA2G7, a biomarker for CVD, was abnormally enhanced in COVID-19 at both nucleotide and protein aspects. These findings provided indications into the prevalence of cardiovascular involvements seen in patients with COVID-19. PLA2G7 could be a potential prognostic and therapeutic target in COVID-19.
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http://dx.doi.org/10.1038/s41598-021-85848-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990942PMC
March 2021

A comprehensive study of raw and roasted macadamia nuts: Lipid profile, physicochemical, nutritional, and sensory properties.

Food Sci Nutr 2021 Mar 27;9(3):1688-1697. Epub 2021 Jan 27.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Oilseeds Processing of Ministry of Agriculture and Hubei Key Laboratory of Lipid Chemistry and Nutrition Wuhan China.

Macadamia nuts have high nutritional value and positive health attributes. Changes to the composition and availability of these compounds during roasting contribute to product quality. In this study, changes to the chemical composition of lipids (fatty acids, triglycerides, and free fatty acids) and other phytochemicals were analyzed, and a sensory evaluation was carried out of two major varieties of macadamia nuts planted in China, after roasting. Only small changes in fatty acid (FA) content and a slight decrease in total triglycerides (TAGs) were observed after roasting. The free fatty acid (FFA) content and the peroxide value were increased by roasting. The total available polyphenol content increased by 25.6% and the oxidative stability index of kernels increased by 21.6%. The sensory scores for taste and aroma were doubled by roasting. Overall, the sensory, nutritional quality, and oxidative stability of roasted macadamia nuts were greatly improved, compared with raw nuts.
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http://dx.doi.org/10.1002/fsn3.2143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958573PMC
March 2021

Pseudotargeted Lipidomics Strategy Enabling Comprehensive Profiling and Precise Lipid Structural Elucidation of Polyunsaturated Lipid-Rich Echium Oil.

J Agric Food Chem 2021 Mar 8. Epub 2021 Mar 8.

Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, People's Republic of China.

Echium oil has great nutritional value as a result of its high content of α-linolenic acid (ALA, 18:3ω-3) and stearidonic acid (SDA, 18:4ω-3). However, the comprehensive lipid profiling and exact structural characterization of bioactive polyunsaturated lipids in echium oil have not yet been obtained. In this study, we developed a novel pseudotargeted lipidomics strategy for comprehensive profiling and lipid structural elucidation of polyunsaturated lipid-rich echium oil. Our approach integrated untargeted lipidomics analysis with a targeted lipidomics strategy based on Paternò-Büchi (PB)-tandem mass spectrometry (MS/MS) using 2-acetylpyridine (2-AP) as the reaction reagent, allowing for high-coverage lipid profiling and simultaneous determination of C═C locations in triacylglycerols (TGs), diacylglycerols (DGs), free fatty acids (FFAs), and sterol esters (SEs) in echium oil. A total of 209 lipid species were profiled, among which 162 unsaturated lipids were identified with C═C location assignment and 42 groups of ω-3 and ω-6 C═C location isomers were discovered. In addition, relative isomer ratios of certain groups of lipid C═C location isomers were revealed. This pseudotargeted lipidomics strategy described in this study is expected to provide new insight into structural characterization of distinctive bioactive lipids in food.
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http://dx.doi.org/10.1021/acs.jafc.0c07268DOI Listing
March 2021

Predictive value of blood eosinophil count in COPD.

Respirology 2021 May 3;26(5):504. Epub 2021 Mar 3.

Respiratory Department, The Second Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou, China.

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http://dx.doi.org/10.1111/resp.14025DOI Listing
May 2021

Combining Multi-Dimensional Convolutional Neural Network (CNN) With Visualization Method for Detection of Glover Infection in Cotton Leaves Using Hyperspectral Imaging.

Front Plant Sci 2021 15;12:604510. Epub 2021 Feb 15.

Key Laboratory of Oasis Ecology Agriculture, Shihezi University, Shihezi, China.

Cotton is a significant economic crop. It is vulnerable to aphids ( Glovers) during the growth period. Rapid and early detection has become an important means to deal with aphids in cotton. In this study, the visible/near-infrared (Vis/NIR) hyperspectral imaging system (376-1044 nm) and machine learning methods were used to identify aphid infection in cotton leaves. Both tall and short cotton plants (Lumianyan 24) were inoculated with aphids, and the corresponding plants without aphids were used as control. The hyperspectral images (HSIs) were acquired five times at an interval of 5 days. The healthy and infected leaves were used to establish the datasets, with each leaf as a sample. The spectra and RGB images of each cotton leaf were extracted from the hyperspectral images for one-dimensional (1D) and two-dimensional (2D) analysis. The hyperspectral images of each leaf were used for three-dimensional (3D) analysis. Convolutional Neural Networks (CNNs) were used for identification and compared with conventional machine learning methods. For the extracted spectra, 1D CNN had a fine classification performance, and the classification accuracy could reach 98%. For RGB images, 2D CNN had a better classification performance. For HSIs, 3D CNN performed moderately and performed better than 2D CNN. On the whole, CNN performed relatively better than conventional machine learning methods. In the process of 1D, 2D, and 3D CNN visualization, the important wavelength ranges were analyzed in 1D and 3D CNN visualization, and the importance of wavelength ranges and spatial regions were analyzed in 2D and 3D CNN visualization. The overall results in this study illustrated the feasibility of using hyperspectral imaging combined with multi-dimensional CNN to detect aphid infection in cotton leaves, providing a new alternative for pest infection detection in plants.
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http://dx.doi.org/10.3389/fpls.2021.604510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917247PMC
February 2021

Exosomal long non-coding RNA LINC00662 promotes non-small cell lung cancer progression by miR-320d/E2F1 axis.

Aging (Albany NY) 2021 02 11;13(4):6010-6024. Epub 2021 Feb 11.

Department of Traditional Chinese Medicine, Shengli Oil Field Central Hospital, Dongying, Shandong Province, China.

Non-small cell lung cancer (NSCLC) is the most common tumor affecting modern people and is associated with severe morbidity and high mortality. Exosomal long non-coding RNAs as crucial regulators are involved in cancer progression. However, the role of exosomal lncRNA LINC00662 in the development of NSCLC remains unclear. Here, we aimed to explore the impact of exosomal lncRNA LINC00662 on the NSCLC progression and the underlying mechanism. Significantly, we revealed that the expression of lncRNA LINC00662 was elevated in the plasma exosome of NSCLC patients. Exosomal LINC00662 promoted proliferation, invasion, and migration, and inhibited apoptosis and cell cycle arrest of NSCLC cells. Mechanically, LINC00662 was able to serve as a miR-320d sponge in NSCLC cells. MiR-320d could target E2F1 in NSCLC cells. Exosomal LINC00662 contributed to the progression of NSCLC by miR-320d/E2F1 axis . Remarkably, exosomal LINC00662 enhanced the tumor growth of NSCLC . Thus, we conclude that exosomal lncRNA LINC00662 promotes NSCLC progression by modulating miR-320d/E2F1 axis. Our finding provides new insights into the mechanism by which exosomal lncRNA LINC00662 contributes to the development of NSCLC. LncRNA LINC00662, miR-320d, and E2F1 may serve as potential targets for NSCLC therapy.
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http://dx.doi.org/10.18632/aging.202522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950287PMC
February 2021

Comprehensive and High-Coverage Lipidomic Analysis of Oilseeds Based on Ultrahigh-Performance Liquid Chromatography Coupled with Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry.

J Agric Food Chem 2021 Feb 2. Epub 2021 Feb 2.

Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Key Laboratory of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture, and Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, People's Republic of China.

Oilseeds are an important source of dietary lipids, and a comprehensive analysis of oilseed lipids is of great significance to human health, while information about the global lipidomes in oilseeds was limited. Herein, an ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry method for comprehensive lipidomic profiling of oilseeds was established and applied. First, the lipid extraction efficiency and lipid coverage of four different lipid extraction methods were compared. The optimized methyl -butyl ether extraction method was superior to isopropanol, Bligh-Dyer, and Folch extraction methods, in terms of the operation simplicity, lipid coverage, and number of identified lipids. Then, global lipidomic analysis of soybean, sesame, peanut, and rapeseed was conducted. A total of 764 lipid molecules, including 260 triacylglycerols, 54 diacylglycerols, 313 glycerophospholipids, 36 saccharolipids, 35 ceramides, 30 free fatty acids, 21 fatty esters, and 15 sphingomyelins were identified and quantified. The compositions and contents of lipids significantly varied among different oilseeds. Our results provided a theoretical basis for the selection and breeding of varieties of oilseed as well as deep processing of oilseed for the edible oil industry.
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http://dx.doi.org/10.1021/acs.jafc.0c07343DOI Listing
February 2021

Thiourea-Grafted Graphite Felts as Positive Electrode for Vanadium Redox Flow Battery.

Front Chem 2020 14;8:626490. Epub 2021 Jan 14.

School of Chemical Engineering, North China University of Science and Technology, Tangshan, China.

In this paper, thiourea was successfully grafted onto the surface of acid preprocessed graphite felts [sulfuric acid-treated graphite felt (SA-GFs)] by thiol-carboxylic acid esterification. The thiourea-grafted graphite felts (TG-GFs) were investigated as the positive electrode for vanadium redox flow battery (VRFB). X-ray photoelectron spectroscopy results suggested that thiourea was grafted into the surface of graphite felts. The cyclic voltammetry showed that the peak potential separation decreased by 0.2 V, and peak currents were greatly enhanced on TG-GF electrode compared with SA-GF electrode, implying improved electro-catalytic activity and reversibility of TG-GF electrode toward VO/ redox reaction. The initial capacity of TG-GF-based cell reached 55.6 mA h at 100 mA cm, 22.6 mA h larger than that of SA-GF-based cell. The voltage and energy efficiency for TG-GF-based cell increased by 4.9% and 4.4% compared with those of SA-GF-based cell at 100 mA cm, respectively.
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http://dx.doi.org/10.3389/fchem.2020.626490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841072PMC
January 2021

Serumal Lipidomics Reveals the Anti-inflammatory Effect of Flax Lignans and Sinapic Acid in High-Fat-Diet-Fed Mice.

J Agric Food Chem 2021 Jan 11. Epub 2021 Jan 11.

Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Key Laboratory of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture, and Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, 2 Xudong Second Road, Wuchang, Wuhan, Hubei 430062, People's Republic of China.

Flax lignans (SDG) and sinapic acid (SA) both have the function of antioxidation and anti-inflammation. However, previous studies have focused mainly on biochemical measurements, gene expression analysis, and clinical assessments. There are limited studies that systematically reveal the underlying mechanism of the anti-inflammation effect of SDG or SA from the lipidomic point of view. Herein, the integrated lipidomic profiling platform was used for the analysis of free fatty acids (FFAs), phospholipids (PLs), triacylglycerols (TAGs), and oxylipins in high-fat (HF)-diet-fed mice after SDG or SA administration. Dietary supplementation of SDG or SA downregulated the levels of total TAGs and FFAs in the ApoE mice model. Furthermore, 28 potential lipids were screened out and considered as key evaluation factors to understand the anti-inflammation function and mechanism of SDG and SA. The results indicated that the anti-inflammatory effect of SDG and SA was principally exerted via regulation of lipid homeostasis.
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http://dx.doi.org/10.1021/acs.jafc.0c07291DOI Listing
January 2021

Urinary coenzyme Q10 as a diagnostic biomarker and predictor of remission in a patient with ADCK4-associated Glomerulopathy: a case report.

BMC Nephrol 2021 01 7;22(1):11. Epub 2021 Jan 7.

Department of Nephrology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Background: AarF domain-containing kinase 4 (ADCK4)-associated glomerulopathy is a mitochondrial nephropathy caused by mutations in the ADCK4 gene, which disrupt coenzyme Q10 biosynthesis.

Case Presentation: We report the case of a 25-year-old female patient with ADCK4-associated glomerulopathy presenting with proteinuria (and with no additional systemic symptoms). A known missense substitution c.737G > A (p.S246N) and a novel frameshift c.577-600del (p.193-200del) mutation were found. We followed the patient for 24 months during supplementation with coenzyme Q10 (20 mg/kg/d - 30 mg/kg/d) and describe the clinical course. In addition, we measured serum and urine coenzyme Q10 levels before and after coenzyme Q10 supplementation and compared them with those of healthy control subjects. The patient's urinary coenzyme Q10 to creatinine ratio was higher than that of healthy controls before coenzyme Q10 supplementation, but decreased consistently with proteinuria after coenzyme Q10 supplementation.

Conclusions: Although the use of urinary coenzyme Q10 as a diagnostic biomarker and predictor of clinical remission in patients with ADCK4-associated glomerulopathy should be confirmed by larger studies, we recommend measuring urinary coenzyme Q10 in patients with isolated proteinuria of unknown cause, since it may provide a diagnostic clue to mitochondrial nephropathy.
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http://dx.doi.org/10.1186/s12882-020-02208-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791994PMC
January 2021

Fluorofenidone Alleviates Renal Fibrosis by Inhibiting Necroptosis Through RIPK3/MLKL Pathway.

Front Pharmacol 2020 16;11:534775. Epub 2020 Dec 16.

Department of Nephrology, Xiangya Hospital, Central South University, Changsha, China.

Cell death and sterile inflammation are major mechanisms of renal fibrosis, which eventually develop into end-stage renal disease. "Necroptosis" is a type of caspase-independent regulated cell death, and sterile inflammatory response caused by tissue injury is strongly related to necrosis. Fluorofenidone (AKF-PD) is a novel compound shown to ameliorate renal fibrosis and associated inflammation. We investigated whether AKF-PD could alleviate renal fibrosis by inhibiting necroptosis. Unilateral ureteral obstruction (UUO) was used to induce renal tubulointerstitial fibrosis in C57BL/6J mice. AKF-PD (500 mg/kg) or necrostatin-1 (Nec-1; 1.65 mg/kg) was administered simultaneously for 3 and 7 days. Obstructed kidneys and serum were harvested after euthanasia. AKF-PD and Nec-1 ameliorated renal tubular damage, inflammatory-cell infiltration, and collagen deposition, and the expression of proinflammatory factors (interlukin-1β, tumor necrosis factor [TNF]-α) and chemokines (monocyte chemoattractant protein-1) decreased. AKF-PD or Nec-1 treatment protected renal tubular epithelial cells from necrosis and reduced the release of lactate dehydrogenase in serum. Simultaneously, production of receptor-interacting protein kinase (RIPK)3 and mixed lineage kinase domain-like protein (MLKL) was also reduced 3 and 7 days after UUO. AKF-PD and Nec-1 significantly decreased the percentage of cell necrosis, inhibiting the phosphorylation of MLKL and RIPK3 in TNF-α- and Z-VAD-stimulated human proximal tubular epithelial (HK-2) cells. In conclusion, AKF-PD and Nec-1 have effective anti-inflammatory and antifibrotic activity in UUO-induced renal tubulointerstitial fibrosis, potentially mediated by the RIPK3/MLKL pathway.
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http://dx.doi.org/10.3389/fphar.2020.534775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772387PMC
December 2020

Convalescent plasma may be a possible treatment for COVID-19: A systematic review.

Int Immunopharmacol 2021 Feb 5;91:107262. Epub 2020 Dec 5.

Department of Pharmacy, China-Japan Union Hospital of Jilin University, Jilin University, Changchun 130033, Jilin, China. Electronic address:

Introduction: The coronavirus disease 2019 (COVID-19) pandemic has spread globally. Therapeutic options including antivirals, anti-inflammatory compounds, and vaccines are still under study. Convalescent plasma(CP) immunotherapy was an effective method for fighting against similar viral infections such as SARS-CoV, and MERS-CoV. In the epidemic of COVID-19, a large number of literatures reported the application of CP. However, there is controversy over the efficacy of CP therapy for COVID-19. This systematic review was designed to evaluate the existing evidence and experience related to CP immunotherapy for COVID-19.

Methods: A literature search was conducted on Pubmed, Cochrane Library, Clinical Key, Wanfang Database; China National Knowledge Infrastructure(CNKI) were used to search for the proper keywords such as SARS-CoV-2, COVID-19, plasma, serum, immunoglobulins, blood transfusion, convalescent, novel coronavirus, immune and the related words for publications published until 15.10.2020. Other available resources were also used to identify relevant articles. The present systematic review was performed based on PRISMA protocol. Data extraction and risk of bias assessments were performed by two reviewers.

Results: Based on the inclusions and exclusions criteria, 45 articles were included in the final review. First, meta-analysis results of RCTs showed that, there were no statistically significant differences between CP transfusion and the control group in terms of reducing mortality(OR 0.79, 95% CI 0.52-1.19, I = 28%) and improving clinical symptoms(OR 1.21, 95%CI 0.68-2.16; I = 0%). The results of controlled NRSIs showed that CP therapy may reduce mortality in COVID-19 patients(RR 0.59, 95% CI 0.53-0.66, I = 0%). Second, limited safety data suggested that CP is a well-tolerated therapy with a low incidence of adverse events. But, due to lack of safety data for the control group, it is really not easy to determine whether CP transfusion has an impact on moderate to serious AEs. Thirdly, for children, pregnant, elderly, tumor and immunocompromised patients, CP may be a well-tolerated therapy, if the disease cannot be controlled and continues to progress. Studies were commonly of low or very low quality.

Conclusions: Although the results of limited RCTs showed that CP cannot significantly reduce mortality, some non-RCTs and case report(series) have found that CP may help patients improve clinical symptoms, clear the virus, and reduce mortality, especially for patients with COVID-19 within ten days of illness. We speculate that CP may be a possible treatment option. High-quality studies are needed for establishing stronger quality of evidence and pharmacists should also be actively involved in the CP treatment process and provide close pharmaceutical care.
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http://dx.doi.org/10.1016/j.intimp.2020.107262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833727PMC
February 2021

Corrigendum: Remdesivir (GS-5734) Impedes Enterovirus Replication Through Viral RNA Synthesis Inhibition.

Front Microbiol 2020 23;11:621197. Epub 2020 Nov 23.

Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi'an, China.

[This corrects the article DOI: 10.3389/fmicb.2020.01105.].
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http://dx.doi.org/10.3389/fmicb.2020.621197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732440PMC
November 2020

Comprehensive Lipidomics Analysis Reveals the Effects of Different Omega-3 Polyunsaturated Fatty Acid-Rich Diets on Egg Yolk Lipids.

J Agric Food Chem 2020 Dec 2;68(50):15048-15060. Epub 2020 Dec 2.

Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Key Laboratory of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture, P. R. China, and Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Xudong 2nd Road, Wuhan, Hubei 430062, P. R. China.

Dietary supplementation with omega-3 polyunsaturated fatty acids (n-3 PUFAs) for laying hens enriches eggs with these essential fatty acids. However, the enrichment patterns and changes to intact lipids in egg yolk have not been sufficiently revealed. Herein, egg yolk lipids from hens fed with diets supplemented with flaxseed, sp. residue, or their mixture were comprehensively analyzed using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). A total of 335 individual lipid species covering 23 (sub)classes were identified and quantified. Distinct n-3 PUFA-lipid profiles were revealed among different groups. Dietary α-linolenic acid (ALA) was mainly deposited in the TAG fraction, whereas synthesized or preformed docosahexaenoic acid (DHA) predominantly existed in the glycerophospholipid form. Furthermore, different lipid species were identified and related lipid pathways after dietary supplementation were analyzed. Collectively, these findings provide us with new knowledge for production, nutritional evaluation, authentication, and application of n-3 PUFA-enriched eggs.
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http://dx.doi.org/10.1021/acs.jafc.0c05215DOI Listing
December 2020

Mineralogical phase transformation of Fe containing sphalerite at acidic environments in the presence of Cu.

J Hazard Mater 2021 02 22;403:124058. Epub 2020 Sep 22.

School of Minerals Processing & Bioengineering, Central South University, Changsha, Hunan, China; Key Lab of Biohydrometallurgy of Ministry of Education, Changsha, Hunan, China.

Dissolution of the exposed sphalerite (marmatite) in abandoned mining sites and tailings may exacerbate acid and metalliferous drainage (AMD) hazards. Cupric ions are inevitable ions in AMD systems but its action mechanism on the dissolution of sphalerite is still unclear. In this work, the possible phase transition from sphalerite to chalcopyrite is firstly discovered in acidic cupric ions solution according to the results of Raman and (synchrotron radiation-based) X-ray (micro-) diffractometer spectra, which should be an important reason that mediates the dissolution of sphalerite. Results of DFT calculations reveal the underlying mechanism that Cu can selectively replace zinc in marmatite lattices and further diffuse into the matrix. Additionally, a strong correlation between the cupric ion consumption with the pH value variation is discussed and the effects of the formed new phase on the dissolution kinetics of marmatite were researched. According to this work, the action mechanism of cupric ions on sphalerite dissolution in acidic environments is furtherly clarified.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124058DOI Listing
February 2021