Publications by authors named "Xin Liu"

4,703 Publications

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Ovomucin may be the key protein involved in the early formation of egg-white thermal gel.

Food Chem 2021 Jul 15;366:130596. Epub 2021 Jul 15.

Meat Processing Key Laboratory of Sichuan Province, School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China. Electronic address:

Clarification of the mechanism of heat-induced gel formation by proteins under natural food systems could provide important references for the regulation of food texture. In the present study, the proteins involved in the early stage (heating at 72 °C for 8 min) of egg-white thermal gel (EWG) formation were studied quantitatively through comparative proteomic analysis. We discovered that the abundance of ovalbumin and ovomucoid increased significantly (p < 0.01), whereas that of ovotransferrin, lysozyme, ovomucin (mucin 5B and mucin 6) decreased significantly (p < 0.01), in the supernatant of EWG. If the initial interaction of egg white proteins was altered by ultrasonic pretreatment, the abundance of ovomucin and lysozyme in the supernatant of EWG increased, and was accompanied by the change from a solid gel to a fluid gel. Based on these results, we hypothesize that ovomucin has a key role in the formation and regulation of EWG properties.
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http://dx.doi.org/10.1016/j.foodchem.2021.130596DOI Listing
July 2021

Correction for: Aucubin exerts anti-osteoporotic effects by promoting osteoblast differentiation.

Aging (Albany NY) 2021 Jul;13(13):17949-17952

School of Life Sciences, Jilin University, Changchun 130012, China.

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http://dx.doi.org/10.18632/aging.203365DOI Listing
July 2021

Incidence of Workplace Violence against Nurses among Chinese Hospitals: A Meta-analysis.

J Nurs Manag 2021 Jul 22. Epub 2021 Jul 22.

School of Nursing, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Aims: To explore the incidence of workplace violence against nurses in Chinese hospitals.

Background: Previous systematic reviews on the incidence of workplace violence against Chinese healthcare workers did not include many articles published in Chinese. Although several studies have investigated cases of violence against healthcare providers in China, no meta-analysis has been conducted to assess the incidence of violence against Chinese nurses.

Evaluation: In this study, relevant data were retrieved from studies published up to July 2020. A meta-analysis was conducted using R software (Version 4.0).

Key Findings: The 12-month incidence of workplace violence among Chinese nurses was 71% (95% CI 67% to 75%) and verbal violence was the most common sub-type of violence (63% 95% CI 58% to 67%).

Conclusion: Chinese nurses are at a high risk of violence at workplace. Hospital managers should explore ways to reduce violence against their employees, especially the younger nurses who work in secondary hospitals.

Implications For Nursing Management: The findings of this study highlight the need to enhance the legal system in terms of laws meant to effectively mitigate violence against nurses in Chinese hospitals. Measures should be particularly taken to protect younger nurses who work in secondary hospitals.
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http://dx.doi.org/10.1111/jonm.13427DOI Listing
July 2021

Analysis of Radiographic Spinopelvic Parameters in Patients With Degenerative Lumbar Kyphoscoliosis.

Geriatr Orthop Surg Rehabil 2021 12;12:21514593211029104. Epub 2021 Jul 12.

Department of Orthopaedics, PeKing University Third Hospital, Beijing Key Laboratory of Spinal Disease Research, Haidian District, Beijing, China.

Purpose: To analyze the relationships between coronal and sagittal spinopelvic parameters in degenerative lumbar kyphoscoliosis (DLKS).

Methods: We enrolled 75 patients with DLKS for a radiographic study between January 2016 and September 2018. Correlations between coronal and sagittal spinopelvic radiographic parameters were analyzed. Then patients were divided into 2 groups: sagittal balanced group (SVA< = 5 cm, 30 patients) and sagittal imbalanced group (SVA >5 cm, 45 patients), and relevant parameters were compared.

Results: The Cobb angle and lumbar lordosis of the DLKS patients were 24.87 ± 11.59° and 17.26 ± 12.24°, respectively. The average age was 68 years old (range: 42-82), and the sex ratio was 2.6:1 (female: 54 patients; male: 21 patients). 50 patients (66.7%) located convexity of the curve at left side, while 25 patients (33.3%) at right side. The Cobb angle correlated with LL-TK (r = -0.228, p = 0.049), LL (r = -0.255, p = 0.027) and SS (r = -0.232, p = 0.045). There were significant differences in PI-LL (t = -3.484, P = 0.001), LL-TK (t = 2.354, P = 0.023), PI (t = -3201, P = 0.002) and PT (t = -2.521, P = 0.014) between sagittal balanced and imbalanced group.

Conclusions: In degenerative lumbar kyphoscoliosis, there are some correlations between coronal and sagittal spinopelvic parameters. Moreover, PI-LL, LL-TK, PI, PT were significantly different between sagittal balanced and imbalanced DLKS patients.
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http://dx.doi.org/10.1177/21514593211029104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278460PMC
July 2021

KRT18 Modulates Alternative Splicing of Genes Involved in Proliferation and Apoptosis Processes in Both Gastric Cancer Cells and Clinical Samples.

Front Genet 2021 5;12:635429. Epub 2021 Jul 5.

Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.

Keratin 18 (KRT18), one of the most abundant keratins in epithelial and endothelial cells, has been reported to be aberrantly expressed in many malignancies and extensively regarded as a biomarker and important regulator in multiple cancers, including gastric cancer (GC). But the molecular regulatory mechanisms of KRT18 in GC patients and cells are largely unknown. In the present study, we analyzed the expression level of KRT18 in 450 stomach adenocarcinoma tissue samples from TCGA database and found a significantly higher expression level in tumor tissues. We then explored the potential functions of KRT18 in AGS cells (human gastric adenocarcinoma cell line) by KRT18 knockdown using siRNA and whole transcriptome RNA-seq analysis. Notably, KRT18 selectively regulates expression of cell proliferation and apoptotic genes. Beyond this, KRT18 affects the alternative splicing of genes enriched in apoptosis, cell cycle, and other cancer-related pathways, which were then validated by reverse transcription-quantitative polymerase chain reaction approach. We validated KRT18-KD promoted apoptosis and inhibited proliferation in AGS cells. We then used RNA-seq data of GC samples to further demonstrate the modulation of KRT18 on alternative splicing regulation. These results together support the conclusion that KRT18 extensively modulates diverse alternative splicing events of genes enriched in proliferation and apoptosis processes. And the dysregulated splicing factors at transcriptional or posttranscriptional level by KRT18 may contribute to the alternative splicing change of many genes, which expands the functional importance of keratins in apoptotic and cell cycle pathways at the posttranscriptional level in GC.
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http://dx.doi.org/10.3389/fgene.2021.635429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287183PMC
July 2021

Enhancement of E-cadherin expression and processing and driving of cancer cell metastasis by ARID1A deficiency.

Oncogene 2021 Jul 21. Epub 2021 Jul 21.

Minhang Hospital, Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical of Sciences, Fudan University, Shanghai, China.

The ARID1A gene, which encodes a subunit of the SWI/SNF chromatin remodeling complex, has been found to be frequently mutated in many human cancer types. However, the function and mechanism of ARID1A in cancer metastasis are still unclear. Here, we show that knockdown of ARID1A increases the ability of breast cancer cells to proliferate, migrate, invade, and metastasize in vivo. The ARID1A-related SWI/SNF complex binds to the second exon of CDH1 and negatively modulates the expression of E-cadherin/CDH1 by recruiting the transcriptional repressor ZEB2 to the CDH1 promoter and excluding the presence of RNA polymerase II. The silencing of CDH1 attenuated the migration, invasion, and metastasis of breast cancer cells in which ARID1A was silenced. ARID1A depletion increased the intracellular enzymatic processing of E-cadherin and the production of C-terminal fragment 2 (CTF2) of E-cadherin, which stabilized β-catenin by competing for binding to the phosphorylation and degradation complex of β-catenin. The matrix metalloproteinase inhibitor GM6001 inhibited the production of CTF2. In zebrafish and nude mice, ARID1A silencing or CTF2 overexpression activated β-catenin signaling and promoted migration/invasion and metastasis of cancer cells in vivo. The inhibitors GM6001, BB94, and ICG-001 suppressed the migration and invasion of cancer cells with ARID1A-deficiency. Our findings provide novel insights into the mechanism of ARID1A metastasis and offer a scientific basis for targeted therapy of ARID1A-deficient cancer cells.
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http://dx.doi.org/10.1038/s41388-021-01930-2DOI Listing
July 2021

m6A Regulator-Associated Modification Patterns and Immune Infiltration of the Tumor Microenvironment in Hepatocarcinoma.

Front Cell Dev Biol 2021 2;9:687756. Epub 2021 Jul 2.

Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Immunotherapy elicits durable responses in many tumors. Nevertheless, the positive response to immunotherapy always depends on the dynamic interactions between the tumor cells and infiltrating lymphocytes in the tumor microenvironment (TME). Currently, the application of immunotherapy in hepatocellular carcinoma (HCC) has achieved limited success. The ectopic modification of N6-methyladenosine (m6A) is a common feature in multiple tumors. However, the relationship between m6A modification with HCC clinical features, prognosis, immune cell infiltration, and immunotherapy efficacy remains unclear. Here, we comprehensively evaluated m6A modification clusters based on 22 m6A regulators and systematically explored the relationship between m6A modification with tumor progression, prognosis, and immune cell infiltration characteristics. The m6Ascore was calculated by principal component analysis to quantify the m6A modifications of individual patients. Key regulators involved in immunoregulation in HCC were identified using immunohistochemistry and immunofluorescence. Three distinct m6A modification clusters were identified. The m6A clusters were significantly associated with clinical features, prognosis, and immune cell infiltration. The three clusters were highly consistent with the three tumor immune phenotypes, i.e., immune-excluded, immune-inflamed, and immune-desert. Comprehensive bioinformatics analysis revealed that high m6Ascore was closely associated with tumor progression, poor prognosis, and immunotherapy non-response. m6A regulators were dysregulated in HCC tissues. Hence, they play a role as predictors of poor prognosis. Tissue microarray demonstrated that overexpressed YTHDF1 was associated with low CD3 and CD8 T cell infiltration in HCC. Our findings demonstrate that m6A modification patterns play a crucial role in the tumor immune microenvironment and the prognosis of HCC. High YTHDF1 expression is closely associated with low CD3 and CD8 T cell infiltration in HCC.
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http://dx.doi.org/10.3389/fcell.2021.687756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283020PMC
July 2021

Comprehensive Analysis of the Immune Infiltrates and PD-L1 of mA RNA Methylation Regulators in Hepatocellular Carcinoma.

Front Cell Dev Biol 2021 30;9:681745. Epub 2021 Jun 30.

Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.

Recently, -methyladenosine (mA) RNA methylation in eukaryotic mRNA has become increasingly obvious in the pathogenesis and prognosis of cancer. Moreover, tumor microenvironment is involved in the regulation of tumorigenesis. In our research, the clinical data, including 374 tumor and 50 normal patients, were obtained from The Cancer Genome Atlas (TCGA). Then 19 mA regulators were selected from other studies. Hepatocellular carcinoma (HCC) patients were clustered in cluster1/2, according to the consensus clustering for the mA RNA regulators. We found that mA regulators were upregulated in cluster1. The cluster1 was associated with higher programmed death ligand 1 (PD-L1) expression level, higher immunoscore, worse prognosis, and distinct immune cell infiltration compared with cluster2. Five risk signatures were identified, including YTH N6-methyladenosine RNA-binding protein 1, YTHDF2, heterogeneous nuclear ribonucleoprotein C, WT1-associated protein, and methyltransferase-like 3, based on univariate Cox and least absolute shrinkage and selection operator regression analysis. High-risk group and low-risk group HCC patients were selected based on the risk score. Similarly, the high-risk group was extremely associated with higher PD-L1 expression level, higher grade, and worse overall survival (OS). Also, cluster1 was mainly enriched in high-risk group. Receiver operating characteristic (ROC) and a nomogram were used to predict the ability and the probability of 3- and 5-year OS of HCC patients. The time-dependent ROC curve (AUC) reached 0.77, 0.67, and 0.68 at 1, 3, and 5 years in the training dataset. Also, AUC areas of 1, 3, and 5 years were 0.7, 0.63, and 0.55 in the validation dataset. The gene set enrichment analysis showed that MTOR signaling pathway and WNT signaling pathway were correlated with cluster1 and high-risk group. Collectively, the research showed that the mA regulators were significantly associated with tumor immune microenvironment in HCC. Risk characteristics based on mA regulators may predict prognosis in patients with HCC and provide a new therapeutic target for improving the efficacy of immunotherapy.
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http://dx.doi.org/10.3389/fcell.2021.681745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277965PMC
June 2021

The Mediating Role of Placental Weight Change in the Association Between Prenatal Exposure to Thallium and Birth Weight: A Prospective Birth Cohort Study.

Front Public Health 2021 2;9:679406. Epub 2021 Jul 2.

School of Public Health, Guangdong Pharmaceutical University, Guangzhou, China.

Previous studies have demonstrated the embryotoxicity and fetotoxicity of thallium (Tl). However, the effects of prenatal exposure to Tl on birth weight and placental weight and the mediating role of placental weight in the association of Tl with birth weight remain unclear. We recruited 2,748 participants from the ongoing Prenatal Environment and Offspring Health Cohort (PEOH Cohort) study, which was initiated in 2016 in Guangzhou, China. The Tl concentrations in maternal urine samples collected during the first and third trimester were determined by inductively coupled plasma mass spectrometry. Birth weight and placental weight were extracted from maternal medical records. Pregnant women exposed to the highest tertile of Tl in the first trimester (β = -42.7 g, 95% CI: -82.3, -3.1 g) and third trimester (β = -50.6 g, 95% CI: -99.0, -2.3 g) had babies with lower birth weights than those exposed to the lowest tertile. We also found significant negative associations of exposure to Tl concentrations in the first and third trimester with placental weight. Mediation analyses showed that 50.3% (95% CI: 15.9, 79.2%) and 33.5% (95% CI: 1.3, 80.3%) of the effects of Tl exposure in the first and third trimester on birth weight were mediated by decreased placental weight. Our results suggest that prenatal exposure to Tl is negatively associated with birth weight and that this association may be mediated by decreased placental weight.
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http://dx.doi.org/10.3389/fpubh.2021.679406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283527PMC
July 2021

RMI2 is a prognostic biomarker and promotes tumor growth in hepatocellular carcinoma.

Clin Exp Med 2021 Jul 18. Epub 2021 Jul 18.

Cancer Center, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan, 430060, China.

Genomic instability is a hallmark of all cancers. RMI2 is a crucial component of the BLM-TopoIIIa-RMI1-RMI2 complex that maintains genome stability. It has been shown to accelerate tumor progression in lung cancer, cervical cancer, and prostate cancer. However, its expression and function in hepatocellular carcinoma (HCC) remain poorly defined. In this study, gene expression data and corresponding clinical information of HCC were downloaded from the TCGA, ICGC, and GEO databases. The expression level and clinical significance of RMI2 in HCC were then analyzed. In addition, cellular and molecular biology experiments were conducted to explore the effects of silencing and overexpression of RMI2 on human liver cancer cells and the associated mechanisms. The results showed that RMI2 expression was elevated in HCC tissues. High expression of RMI2 was correlated with shorter survival and poor prognosis of patients. The results of CCK-8, Edu, and clonogenic assays confirmed that RMI2 overexpression promoted the proliferation of HCC cells. Flow cytometric analysis demonstrated that RMI2 overexpression enhanced G1-S phase transition and decreased apoptosis. Moreover, the protein expression of key effector molecules in the p53 signaling pathway was reduced following RMI2 overexpression. In summary, these results indicate that RMI2 promotes the growth of HCC cells and suppresses their apoptosis by inhibiting the p53 signaling pathway. This study provides new insights into the mechanisms driving HCC tumorigenesis and new therapeutic targets.
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http://dx.doi.org/10.1007/s10238-021-00742-8DOI Listing
July 2021

The PHU-NET: A robust phase unwrapping method for MRI based on deep learning.

Magn Reson Med 2021 Jul 17. Epub 2021 Jul 17.

Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Purpose: This work was aimed at designing a deep-learning-based approach for MR image phase unwrapping to improve the robustness and efficiency of traditional methods.

Methods: A deep learning network called PHU-NET was designed for MR phase unwrapping. In this network, a novel training data generation method was proposed to simulate the wrapping patterns in MR phase images. The wrapping boundary and wrapping counts were explicitly estimated and used for network training. The proposed method was quantitatively evaluated and compared to other methods using a number of simulated datasets with varying signal-to-noise ratio (SNR) and MR phase images from various parts of the human body.

Results: The results showed that our method performed better in the simulated data even under an extremely low SNR. The proposed method had less residual wrapping in the images from various parts of human body and worked well in the presence of severe anatomical discontinuity. Our method was also advantageous in terms of computational efficiency compared to the traditional methods.

Conclusion: This work proposed a robust and computationally efficient MR phase unwrapping method based on a deep learning network, which has promising performance in applications using MR phase information.
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http://dx.doi.org/10.1002/mrm.28927DOI Listing
July 2021

Pillared Metal-Organic Framework Initiating Intermolecular Atom-Transfer Radical Addition via Visible-Light-Induced Electron Transfer Activation of Haloalkanes.

ACS Appl Mater Interfaces 2021 Jul 16. Epub 2021 Jul 16.

Laboratory of Advanced Energy Materials, College of Chemistry, Northeast Normal University, Changchun 130021, P. R. China.

Herein, a novel metal-organic framework (MOF) with a pillared-layer structure was rationally synthesized to initiate intermolecular atom-transfer radical addition (ATRA) via photoinduced electron transfer activation of haloalkanes. The MOF synthesized via the controllable pillared-layer method is of excellent visible-light absorption and high chemical stability. Photocatalytic experiments show the atom transfer of various alkyl halides (R-X, X = Cl/Br/I) onto diverse olefins was successfully achieved to produce functional ATRA products. The mechanism and experimental investigations reveal the prepared MOF serves as an efficient photocatalyst with strong reduction potential to activate haloalkane substrates via photoinduced electron transfer, generating a highly reactive alkyl radical to trigger the ATRA reaction. Key events in the ATRA reaction, including alkyl radical photogeneration as well as halide transfer, have been further regulated to achieve preferable photocatalytic performance with higher yields, shorter reaction time, and desirable cycling capability. It is notable that the work is the first report on photoinduced electron transfer activation of halides by a MOF photocatalyst for the ATRA reaction, providing a new blueprint for MOFs to develop photoinduced radical reactions.
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http://dx.doi.org/10.1021/acsami.1c06391DOI Listing
July 2021

[Ag(NH3)2]2SO4: A strategy for the coordination of cationic moieties to design nonlinear optical materials.

Angew Chem Int Ed Engl 2021 Jul 15. Epub 2021 Jul 15.

Beijing Normal University, College of Chemistry, CHINA.

Over recent decades, guided by the anionic group theory of nonlinear optical (NLO) materials, rational design strategies have been primarily focused on anionic moieties; consequently, structural modification and design of cationic moieties have long been neglected. Herein, we report a strategy for the coordination of cationic moieties that substantially enhances the nonlinear and linear optical properties of NLO materials. For an example with well-known crystal structure, [Ag(NH 3 ) 2 ] 2 SO 4 , we demonstrate that the coordination of the Ag + cation by the neutral ligand drives the formation of a noncentrosymmetric tetragonal P 2 1 c structure as a positive uniaxial crystal. The bending of the [Ag(NH 3 ) 2 ] + cationic moiety parallel to the z- axis generates an anisotropic arrangement of the dipoles, i.e., a dipole of 0.12 D along the z -direction, yet zero dipole in the xy -plane, which interacts anisotropically with the incident light oscillating electromagnetic wave, leading to optical anisotropy with a large birefringence. The incident beam of 589.3 nm normal to the (110) crystal plane measures ∆ n obv. = 0.08, and [Ag(NH 3 ) 2 ] 2 SO 4 also exhibits a phase-matching NLO response 1.4 times that of KH 2 PO 4 (KDP) ( obv . 1.4 × KDP @1064 nm; cal . d 36 = 1.50 pm/V). These discoveries will illuminate future explorations of other functional ionic materials.
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http://dx.doi.org/10.1002/anie.202107780DOI Listing
July 2021

Comparative analysis of clonal evolution among patients with right- and left-sided colon and rectal cancer.

iScience 2021 Jul 11;24(7):102718. Epub 2021 Jun 11.

BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China.

Tumor multiregion sequencing reveals intratumor heterogeneity (ITH) and clonal evolution playing a key role in tumor progression and metastases. Large-scale high-depth multiregional sequencing of colorectal cancer, comparative analysis among patients with right-sided colon cancer (RCC), left-sided colon cancer (LCC), and rectal cancer (RC), as well as the study of lymph node metastasis (LN) with extranodal tumor deposits (ENTDs) from evolutionary perspective remain weakly explored. Here, we recruited 68 patients with RCC (18), LCC (20), and RC (30). We performed high-depth whole-exome sequencing of 206 tumor regions including 176 primary tumors, 19 LN, and 11 ENTD samples. Our results showed ITH with a Darwinian pattern of evolution and the evolution pattern of LCC and RC was more complex and divergent than RCC. Genetic and evolutionary evidences found that both LN and ENTD originated from different clones. Moreover, ENTD was a distinct entity from LN and evolved later.
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http://dx.doi.org/10.1016/j.isci.2021.102718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254024PMC
July 2021

The Association of Fried Meat Consumption With the Gut Microbiota and Fecal Metabolites and Its Impact on Glucose Homoeostasis, Intestinal Endotoxin Levels, and Systemic Inflammation: A Randomized Controlled-Feeding Trial.

Diabetes Care 2021 Jul 12. Epub 2021 Jul 12.

Department of Nutrition and Food Hygiene, National Key Discipline, School of Public Health, Harbin Medical University, Harbin, China

Objective: This randomized controlled-feeding trial aimed to determine the impact of fried meat intake on the gut microbiota and fecal cometabolites and whether such impacts influenced host glucose homoeostasis, intestinal endotoxin levels, and systemic inflammation.

Research Design And Methods: A total of 117 overweight adults were randomized into two groups. Fifty-nine participants were provided fried meat four times per week, and 58 participants were restricted from fried meat intake, while holding food group and nutrient compositions constant, for 4 weeks. The gut microbiota was analyzed by 16S rRNA sequencing. Glucose and insulin concentrations at 0, 30, 60, and 120 min of an oral glucose tolerance test, fecal microbiota-host cometabolite levels, and intestinal endotoxin and inflammation serum biomarker levels were measured. The area under the curve (AUC) for insulin, insulinogenic index (IGI), and muscle insulin resistance index (MIRI) were calculated.

Results: The participants who consumed fried meat had lower IGI values than the control subjects, but they had higher MIRI and AUC values of insulin and lipopolysaccharide (LPS), TNF-α, IL-10, and IL-1β levels ( < 0.05). Fried meat intake lowered microbial community richness and decreased and abundances while increasing , , and abundances ( FDR <0.05), provoking a significant shift in the fecal cometabolite profile, with lower 3-indolepropionic acid, valeric acid, and butyric acid concentrations and higher carnitine and methylglutaric acid concentrations ( FDR <0.05). Changes in these cometabolite levels were significantly associated with changes in IGI and MIRI values and LPS, FGF21, TNF-α, IL-1β, and IL-10 levels ( < 0.05).

Conclusions: Fried meat intake impaired glucose homoeostasis and increased intestinal endotoxin and systemic inflammation levels by influencing the gut microbiota and microbial-host cometabolites.
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http://dx.doi.org/10.2337/dc21-0099DOI Listing
July 2021

Technical Note: A preliminary study of dual-tracer PET image reconstruction guided by FDG and/or MR kernels.

Med Phys 2021 Jul 12. Epub 2021 Jul 12.

Lauterbur Research Center for Biomedical Imaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Purpose: Clinically, single radiotracer positron emission tomography (PET) imaging is a commonly used examination method; however, since each radioactive tracer reflects the information of only one kind of cell, it easily causes false negatives or false positives in disease diagnosis. Therefore, reasonably combining two or more radiotracers is recommended to improve the accuracy of diagnosis and the sensitivity and specificity of the disease when conditions permit.

Methods: This paper proposes incorporating F-fluorodeoxyglucose (FDG) as a higher-quality PET image to guide the reconstruction of other lower-count C-methionine (MET) PET datasets to compensate for the lower image quality by using a popular kernel algorithm. Specifically, the FDG prior is needed to extract kernel features, and these features were used to build a kernel matrix using a k-nearest-neighbor (kNN) search for MET image reconstruction. We created a 2-D brain phantom to validate the proposed method by simulating sinogram data containing Poisson random noise and quantitatively compared the performance of the proposed FDG-guided kernelized expectation maximization (KEM) method with the performance of Gaussian and non-local means (NLM) smoothed maximum likelihood expectation maximization (MLEM), MR-guided KEM and multi-guided-S KEM algorithms. Mismatch experiments between FDG/MR and MET data were also carried out to investigate the outcomes of possible clinical situations.

Results: In the simulation study, the proposed method outperformed the other algorithms by at least 3.11% in the signal-to-noise ratio (SNR) and 0.68% in the contrast recovery coefficient (CRC), and it reduced the mean absolute error (MAE) by 8.07%. Regarding the tumor in the reconstructed image, the proposed method contained more pathological information. Furthermore, the proposed method was still superior to the MR-guided KEM method in the mismatch experiments.

Conclusions: The proposed FDG-guided KEM algorithm can effectively utilize and compensate for the tissue metabolism information obtained from dual-tracer PET to maximize the advantages of PET imaging.
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http://dx.doi.org/10.1002/mp.15089DOI Listing
July 2021

Learned Low-rank Priors in Dynamic MR Imaging.

IEEE Trans Med Imaging 2021 Jul 12;PP. Epub 2021 Jul 12.

Deep learning methods have achieved attractive performance in dynamic MR cine imaging. However, most of these methods are driven only by the sparse prior of MR images, while the important low-rank (LR) prior of dynamic MR cine images is not explored, which may limit further improvements in dynamic MR reconstruction. In this paper, a learned singular value thresholding (Learned-SVT) operator is proposed to explore low-rank priors in dynamic MR imaging to obtain improved reconstruction results. In particular, we put forward a model-based unrolling sparse and low-rank network for dynamic MR imaging, dubbed as SLR-Net. SLR-Net is defined over a deep network flow graph, which is unrolled from the iterative procedures in the iterative shrinkage-thresholding algorithm (ISTA) for optimizing a sparse and LR-based dynamic MRI model. Experimental results on a single-coil scenario show that the proposed SLR-Net can further improve the state-of-the-art compressed sensing (CS) methods and sparsity-driven deep learning-based methods with strong robustness to different undersampling patterns, both qualitatively and quantitatively. Besides, SLR-Net has been extended to a multi-coil scenario, and achieved excellent reconstruction results compared with a sparsity-driven multi-coil deep learning-based method under a high acceleration. Prospective reconstruction results on an open real-time dataset further demonstrate the capability and flexibility of the proposed method on real-time scenarios.
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http://dx.doi.org/10.1109/TMI.2021.3096218DOI Listing
July 2021

An enhanced methodology for predicting protein-protein interactions between human and hepatitis C virus via ensemble learning algorithms.

J Biomol Struct Dyn 2021 Jul 11:1-11. Epub 2021 Jul 11.

Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, Xuzhou, Jiangsu, China.

Hepatitis C virus (HCV) is responsible for a variety of human life-threatening diseases, which include liver cirrhosis, chronic hepatitis, fibrosis and hepatocellular carcinoma (HCC) . Computational study of protein-protein interactions between human and HCV could boost the findings of antiviral drugs in HCV therapy and might optimize the treatment procedures for HCV infections. In this analysis, we constructed a prediction model for protein-protein interactions between HCV and human by incorporating the features generated by pseudo amino acid compositions, which were then carried out at two levels: categories and features. In brief, extra-tree was initially used for feature selection while SVM was then used to build the classification model. After that, the most suitable models for each category and each feature were selected by comparing with the three ensemble learning algorithms, that is, Random Forest, Adaboost, and Xgboost. According to our results, profile-based features were more suitable for building predictive models among the four categories. AUC value of the model constructed by Xgboost algorithm on independent data set could reach 92.66%. Moreover, Distance-based Residue, Physicochemical Distance Transformation and Profile-based Physicochemical Distance Transformation performed much better among the 17 features. AUC value of the Adaboost classifier constructed by Profile-based Physicochemical Distance Transformation on the independent dataset achieved 93.74%. Taken together, we proposed a better model with improved prediction capacity for protein-protein interactions between human and HCV in this study, which could provide practical reference for further experimental investigation into HCV-related diseases in future.Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2021.1946429DOI Listing
July 2021

Verifying earplug attenuation and evaluating the effectiveness of one-on-one training along with earplug fit testing at nine facilities in China.

Am J Ind Med 2021 Jul 11. Epub 2021 Jul 11.

Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China.

Background: The purpose of this study was twofold: (1) to measure the personal attenuation ratings (PARs) in Chinese workers wearing hearing protection devices (HPDs), to evaluate the effectiveness of the single number rating (SNR), the noise reduction rating (NRR), and the associated derated values of earplugs; and (2) to evaluate the effectiveness of one-on-one training along with earplug fit testing on PAR improvement.

Methods: Noise exposure measurements, one-on-one training, and fit tests to measure earplug attenuation were conducted at nine manufacturing facilities located in eastern China from 2016 to 2017. 503 workers participated in the study. Ninety-three percent were male. 199 workers were provided one-on-one training.

Results: Before training, 14% and 15% of the workers achieved the attenuation predicted by the manufacturer's SNR and NRR, 56% and 65% exceeded the derated SNR and NRR, respectively. Following one-on-one training, mean PAR improved significantly by 15 dB (p < 0.01); 26% of the workers achieved SNR and NRR, 79% and 91% met the associated derated values, respectively.

Conclusions: Labeled noise attenuation ratings and their derated values overestimated the actual level of attenuation among workers wearing HPDs. One-on-one training along with earplug fit testing contributed to improved PARs.
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http://dx.doi.org/10.1002/ajim.23270DOI Listing
July 2021

Identification and Expression Profiling Analysis of the Cation/Ca Exchanger (CCX) Gene Family: Overexpression of SlCCX1-LIKE Regulates the Leaf Senescence in Tomato Flowering Phase.

Front Genet 2021 25;12:683904. Epub 2021 Jun 25.

Key Laboratory of Protected Horticulture of Education Ministry, College of Horticulture, Shenyang Agricultural University, Shenyang, China.

Cation gradients in plant cellular compartments are maintained by the synergistic actions of various ion exchangers, pumps, and channels. Cation/Ca exchanger (CCX) is one of the clades of the Ca/cation antiporter super family. Here, five genes were identified in tomato. Sequence analysis indicated that SlCCXs have the conserved motifs as the CCX domain. Analysis of the expression level of each member of tomato CCX gene family under cation (Mg, Mn, Na, and Ca) treatment was determined by qRT-PCR. Tomato CCX demonstrated different degrees of responding to cation treatment. Changes in expression was induced by Mg and Mn treatment. Analysis of the expression of genes in different tissues demonstrated that constitutive high expression of a few genes, including and , indicated their role in tomato organ growth and development. Overexpression of dramatically induced leaf senescence. Transcriptome analysis showed that genes related to ROS and several IAA signaling pathways were significantly downregulated, whereas ETH and ABA signaling pathway-related genes were upregulated in overexpression of () plants, compared with the wild type (WT). Moreover, overexpression of plants accumulated more ROS content but less Mg content. Collectively, the findings of this study provide insights into the base mechanism through which CCXs regulate leaf senescence in tomato.
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http://dx.doi.org/10.3389/fgene.2021.683904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270643PMC
June 2021

T-Weighted Whole-Brain Intracranial Vessel Wall Imaging at 3 Tesla With Cerebrospinal Fluid Suppression.

Front Neurosci 2021 25;15:665076. Epub 2021 Jun 25.

Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Background: T-weighted (Tw) intracranial vessel wall imaging (IVWI) provides good contrast to differentiate intracranial vasculopathies and discriminate various important plaque components. However, the strong cerebrospinal fluid (CSF) signal in Tw images interferes with depicting the intracranial vessel wall. In this study, we propose a T-prepared sequence for whole-brain IVWI at 3T with CSF suppression.

Methods: A preparation module that combines T preparation and inversion recovery (TIR) was used to suppress the CSF signal and was incorporated into the commercial three-dimensional (3D) turbo spin echo sequence-Sampling Perfection with Application optimized Contrast using different flip angle Evolution (SPACE). This new technique (hereafter called TIR-SPACE) was evaluated on nine healthy volunteers and compared with two other commonly used 3D T-weighted sequences: Tw-SPACE and FLAIR-SPACE (FLAIR: fluid-attenuated inversion recovery). The signal-to-noise ratios (SNRs) of the vessel wall (VW) and CSF and contrast-to-noise ratios (CNRs) between them were measured and compared among these three T-weighted sequences. Subjective wall visualization of the three T-weighted sequences was scored blindly and independently by two radiologists using a four-point scale followed by inter-rater reproducibility analysis. A pilot study of four stroke patients was performed to preliminarily evaluate the diagnostic value of this new sequence, which was compared with two conventional T-weighted sequences.

Results: TIR-SPACE had the highest CNR (11.01 ± 6.75) compared with FLAIR-SPACE (4.49 ± 3.15; < 0.001) and Tw-SPACE (-56.16 ± 18.58; < 0.001). The subjective wall visualization score of TIR-SPACE was higher than those of FLAIR-SPACE and Tw-SPACE (TIR-SPACE: 2.35 ± 0.59; FLAIR-SPACE: 0.52 ± 0.54; Tw-SPACE: 1.67 ± 0.58); the two radiologists' scores showed excellent agreement (ICC = 0.883).

Conclusion: The TIR preparation module markedly suppressed the CSF signal without much SNR loss of the other tissues (i.e., vessel wall, white matter, and gray matter) compared with the IR pulse. Our results suggest that TIR-SPACE is a potential alternative T-weighted sequence for assessing intracranial vascular diseases.
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http://dx.doi.org/10.3389/fnins.2021.665076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267868PMC
June 2021

A chromosome-level genome of the agile gracile mouse opossum (Gracilinanus Agilis).

Genome Biol Evol 2021 Jul 10. Epub 2021 Jul 10.

Integrative Biology Laboratory, College of Life Sciences, Nanjing Normal University, Nanjing, China.

There are more than 100 species of American didelphid marsupials (opossums and mouse opossums). Limited genomic resources for didelphids exists, with only two publicly available genome assemblies compared to dozens in the case of their Australasian counterparts. This discrepancy impedes evolutionary and ecological research. To address this gap, we assembled a high-quality chromosome-level genome of the agile gracile mouse opossum (Gracilinanus agilis) using a combination of stLFR sequencing, polishing with mate-pair data, and anchoring onto pseudochromosomes using Hi-C. This species employs a rare life-history strategy, semelparity, and all G. agilis males and most females die at the end of their first breeding season after succumbing to stress and exhaustion. The 3.7-Gb chromosome-level assembly, with 92.6% anchored onto pseudochromosomes, has a scaffold N50 of 683.5 Mb and a contig N50 of 56.9 kb. The genome assembly show high completeness, with a mammalian BUSCO score of 88.1%. Around 49.7% of the genome contains repetitive elements. Gene annotation yielded 24,425 genes, of which 83.9% were functionally annotated. The G. agilis genome is an important resource for future studies of marsupial biology, evolution, and conservation.
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http://dx.doi.org/10.1093/gbe/evab162DOI Listing
July 2021

Lactobionic acid-modified phycocyanin nanoparticles loaded with doxorubicin for synergistic chemo-photodynamic therapy.

Int J Biol Macromol 2021 Jul 9;186:206-217. Epub 2021 Jul 9.

Engineering Research Center for Biomedical Materials, Anhui Key Laboratory of Modern Biomanufacturing, School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui Province 230601, PR China. Electronic address:

Phycocyanin (PC) is considered to be an effective natural photosensitizer, but it has not been well utilized as its inefficient biostability and intracellular accumulation. To overcome these limitations, the nano-sized PC particles (LAPC/DOX) were developed by grafting with lactobionic acid (LA) and loading with doxorubicin (DOX). Compared to the PC solution, the storage-stability and photostability of PC particles were remarkably increased, and the formation of nanoparticles further improved its biostability. Besides, CLSM images confirmed that LA could also enhance cellular uptake, resulting in more intracellular PC and DOX accumulation. MTT assay revealed that LAPC/DOX caused the highest cytotoxicity by combined chemo-photodynamic therapy. Finally, LAPC/DOX could efficiently accumulate and spread in tumoral multicellular spheroids, resulting in the enhanced growth inhibition. Overall, the LAPC/DOX is effective in cancer treatment, which provides new insights for the usage of functional proteins in vivo.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.047DOI Listing
July 2021

Air quality benefits of achieving carbon neutrality in China.

Sci Total Environ 2021 Jun 30;795:148784. Epub 2021 Jun 30.

Center of Air Quality Simulation and System Analysis, Chinese Academy of Environmental Planning, 100012 Beijing, China; State Environmental Protection Key Laboratory of Environmental Planning and Policy Simulation, Chinese Academy of Environmental Planning, 100012 Beijing, China. Electronic address:

Achieving carbon neutrality before 2060 newly announced in China are expected to substantially affect air quality. Here we project the pollutants emissions in China based on a carbon neutrality roadmap and clean air policies evolution; national and regional PM and O concentrations in 2030 (the target year of carbon peak), 2035 (the target year of "Beautiful China 2035" launched by the Chinese government to fundamentally improve air quality) and 2060 (the target year of carbon neutrality) are then simulated using an air quality model. Results showed that compared with 2019, emissions of SO, NO, primary PM, and VOCs are projected to reduce by 42%, 42%, 44%, and 28% in 2030, by 57%, 58%, 60%, and 42% in 2035, by 93%, 93%, 90% and 61% in 2060 respectively. Consequently, in 2030, 2035, and 2060, the national annual mean PM will be 27, 23, and 11 μg m; and the 90th percentile of daily 8-h maxima of O (O-8h 90th) will be 129, 123, and 93 μg m; 82%, 94%, and 100% of 337 municipal cities will reach the current national air quality standard, respectively. It's expected that the "Beautiful China 2035" target is very likely to be achieved, and about half of the 337 cities will meet the current WHO air quality guideline in 2060. In the near future, strict environmental policies driven by "Beautiful China 2035" are needed due to their substantial contribution to emission reductions. By 2060, the low-carbon policies driven by the carbon neutrality target are expected to contribute to larger than 80% of reductions in PM and O-8h 90th concentrations relative to the 2020 levels, implying that more attention could be paid to low-carbon policies after 2035. Our research would provide implications for future co-governance of air pollution and climate change mitigation in China and other developing countries.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148784DOI Listing
June 2021

Erratum: Long noncoding RNA LINC01123 promotes the proliferation and invasion of hepatocellular carcinoma cells by modulating the miR-34a-5p/TUFT1 axis: Erratum.

Int J Biol Sci 2021 8;17(9):2336-2337. Epub 2021 Jun 8.

Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Zhejiang Provincial People's Hospital (People's Hospital of Hangzhou Medical College), Hangzhou, Zhejiang 310014, China.

[This corrects the article DOI: 10.7150/ijbs.45457.].
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http://dx.doi.org/10.7150/ijbs.61069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241735PMC
June 2021

LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis.

Cell Mol Biol Lett 2021 Jul 8;26(1):33. Epub 2021 Jul 8.

Handan Central Hospital, Handan, 056001, Hebei Province, China.

Background: Long noncoding RNA (lncRNA), urothelial carcinoma-associated 1 (UCA1) is aberrantly expressed in multiple cancers and has been verified as an oncogene. However, the underlying mechanism of UCA1 in the development of gastric cancer is not fully understood. In the present study, we aimed to identify how UCA1 promotes gastric cancer development.

Methods: The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data were used to analyze UCA1 and myosin VI (MYO6) expression in gastric cancer. Western blot and quantitative real-time PCR (QPCR) were performed to test the expression level of the UCA1/miR-145/MYO6 axis in gastric cancer cell lines and tissues. The roles of the UCA1/miR-145/MYO6 axis in gastric cancer in vitro and in vivo were investigated by CCK-8 assay, flow cytometry, siRNAs, immunohistochemistry, and a mouse xenograft model. The targeted relationship among UCA1, miR-145, and MYO6 was predicted using LncBase Predicted v.2 and TargetScan online software, and then verified by luciferase activity assay and RNA immunoprecipitation.

Results: UCA1 expression was higher but miR-145 expression was lower in gastric cancer cell lines or tissues, compared to the adjacent normal cell line or normal tissues. Function analysis verified that UCA1 promoted cell proliferation and inhibited cell apoptosis in the gastric cancer cells in vitro and in vivo. Mechanistically, UCA1 could bind directly to miR-145, and MYO6 was found to be a downstream target gene of miR-145. miR-145 mimics or MYO6 siRNAs could partly reverse the effect of UCA1 on gastric cancer cells.

Conclusions: UCA1 accelerated cell proliferation and inhibited cell apoptosis through sponging miR-145 to upregulate MYO6 expression in gastric cancer, indicating that the UCA1/miR-145/MYO6 axis may serve as a potential therapeutic target for gastric cancer.
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http://dx.doi.org/10.1186/s11658-021-00275-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268585PMC
July 2021

Simultaneous visual diagnosis of acute hepatopancreatic necrosis disease and Enterocytozoon hepatopenaei infection in shrimp with duplex recombinase polymerase amplification.

J Fish Dis 2021 Jul 8. Epub 2021 Jul 8.

Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Biomedical Engineering, College of Life Science and Technology, Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China.

Shrimp is a globally popular seafood. Shrimp farming has been challenged by various infectious diseases that lead to significant economic losses. The prevention of two important shrimp infectious diseases, the acute hepatopancreatic necrosis disease (AHPND) and the Enterocytozoon hepatopenaei (EHP) infection, is highly dependent on early and accurate diagnostic. On-site monitoring of the two diseases in shrimp farming facilities demands point-of-care-testing (POCT) type of diagnostic assays. This study established a duplex recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) combined assay that could simultaneously diagnose the two diseases. The optimized RPA-LFD assay could finish the diagnostic in 35 min with good specificity, and the sensitivity reached 10 and 10 gene copies per reaction for EHP and AHPND, respectively, which were at the same level as the currently available molecular diagnostic assays. Test results of clinical samples showed 100% agreement of this assay with the industrial standard nested polymerase chain reaction (PCR) assays, and samples with both diseases were simultaneously identified. Because of the isothermal 37℃ amplification and the visual reading of the signal on dipsticks, the dependence on equipment is minimal. This duplex RPA-LFD assay is well suited for simultaneous POCT diagnostic of the two important shrimp infectious diseases. Moreover, the principle can be applied to multiplex POCT diagnostic of other infectious diseases in aquaculture.
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http://dx.doi.org/10.1111/jfd.13492DOI Listing
July 2021

A comparison analysis of the decoupling carbon emissions from economic growth in three industries of Heilongjiang province in China.

Authors:
Xin Liu Shunlong Li

Environ Sci Pollut Res Int 2021 Jul 6. Epub 2021 Jul 6.

College of Economics and Management, Northeast Forestry University, Harbin, China.

Growing environmental pressure urges China to develop in a sustainable and low carbon way, and thus China strives to achieve a carbon peak by 2030 and carbon neutrality by 2060. As the main energy and heavy industry based in China, carbon emissions in Heilongjiang Provice play a crucial role in achieving China's climate change targets. Compared with countries with similar populations, Heilongjiang Province (HLJP)'s carbon emissions are still at a low level. In the research on Heilongjiang Province's carbon emissions among three industries, we apply Tapio decoupling and the Logarithmic Mean Divisia Index (LMDI) method to analyze the decoupling elasticity and effort index of three industries' growth and carbon emissions in HLJP from 2005 to 2017. Moreover, Deng's grey relation model is adopted to judge the relation degree between industries and carbon emissions. The results show that: (1) with respect to decoupling, the secondary industry is in a relatively stable state of decoupling; however, the decoupling state of the primary industry is not stable at all, which appears expansive or negative in the studies over the past years. (2) The carbon emissions of three industries show an upward trend during the study period, of which the economic scale effect contributes most, while the energy intensity was the main inhibitor to carbon emissions. (3) Three industrial-economic structures are related to carbon emissions at a high level. The tertiary industry ranks the first, which is followed by the secondary industry and then the primary industry. The economic growth of the tertiary industry is much faster than the other two, hence, its relation degree is the highest. Overall, our analysis can provide an effective reference for the decline of carbon emissions in each industry.
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http://dx.doi.org/10.1007/s11356-021-15241-2DOI Listing
July 2021

Resveratrol prevents inflammation and oxidative stress response in LPS-induced human gingival fibroblasts by targeting the PI3K/AKT and Wnt/β-catenin signaling pathways.

Genet Mol Biol 2021 2;44(3):e20200349. Epub 2021 Jul 2.

Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, P.R. China.

This study aimed to elucidate the anti-inflammatory and antioxidant properties of resveratrol (RSV) in human gingival fibroblasts (HGFs) following stimulation by P. gingivalis lipopolysaccharide (LPS). The levels of the inflammatory cytokines IL-1β, IL-6, IL-8 and TNFα, the activity of the antioxidant enzymes SOD and GSH-Px, and the levels of MDA, were evaluated by ELISA. It was observed that the expression of IL-1β, IL-6, IL-8 and TNFα in LPS-induced HGFs was significantly downregulated by RSV in a dose-dependent manner. RSV also partly increased oxidative stress (OS)-related factors, including SOD and GSH-Px, which was accompanied by a decrease in MDA production, although the results were not statistically significant. Additionally, RSV-induced deactivation of the PI3K/AKT and Wnt/β-catenin pathways in LPS-induced HGFs was observed by western blot analysis. Subsequently, it was demonstrated treatment with PI3K/AKT pathway inhibitor (LY294002) or Wnt/β-catenin pathway inhibitor (Dickkopf-1, DKK-1) could further enhance the anti-inflammatory and antioxidant effects of RSV by downregulating the expression of IL-1β, IL-6, IL-8 and TNFα, and the production of MDA, and increasing the activity of SOD and GSH-Px in LPS-induced HGFs. These results suggested RSV attenuated the inflammation and OS injury of P. gingivalis LPS-treated HGFs by deactivating the PI3K/AKT and Wnt/β-catenin signaling pathways.
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http://dx.doi.org/10.1590/1678-4685-GMB-2020-0349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258621PMC
July 2021

Nutrient consumption patterns of Lactobacillus plantarum and their application in suancai.

Int J Food Microbiol 2021 Jun 29;354:109317. Epub 2021 Jun 29.

Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, Heilongjiang, China. Electronic address:

The purpose of the present study was to control the fermentation time and nitrite content of suancai prepared with Lactobacillus plantarum. According to analyses of the consumption amount and rate of nutrients, growth-stimulating nutrients, essential nutrients and nutrients accelerating the fermentation process of suancai, Asp, Thr, Glu, Cys, Tyr, Mg, Mn and inosine were selected as additions to suancai prepared with L. plantarum. The fermentation time and nitrite content of suancai supplemented with nutrients and prepared with L. plantarum were shortened by 2 days and 5 days and reduced by approximately 0.1-fold and 0.7-fold, respectively, compared with unsupplemented suancai prepared with L. plantarum at 25 °C and 10 °C. The fermentation time and nitrite content of suancai supplemented with nutrients and prepared with L. plantarum were shortened by 6 days and 15 days and reduced by approximately 0.17-fold and 0.8-fold, respectively, compared with suancai undergoing spontaneous fermentation at 25 °C and 10 °C. Furthermore, no significant differences were observed in sensory properties in suancai. The results of this study indicated that certain nutrients accelerated the growth of L. plantarum and reduced the fermentation time and nitrite content of suancai prepared with L. plantarum. These findings help to establish a foundation for the practical use of nutrients to control the fermentation of suancai.
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http://dx.doi.org/10.1016/j.ijfoodmicro.2021.109317DOI Listing
June 2021