Publications by authors named "Xin Lü"

1,423 Publications

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TMT-labeled quantitative proteomic analysis to identify proteins associated with the stability of peanut milk.

J Sci Food Agric 2021 May 14. Epub 2021 May 14.

Institute of Agricultural and Sideline Products Processing, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.

Background: Peanut milk benefits human health mainly due to its high protein content and suitable amino acid composition. To reveal the molecular mechanism affecting the quality of peanut milk, tandem mass tag (TMT)-labeled proteomic analysis was applied to identify the proteome variation between two peanut cultivars that produced peanut milk with the best and worst stability.

Results: A total of 478 differentially abundant proteins (fold change>1.2 or < 0.83, p < 0.05) were identified. Most of these proteins were located in the cytoplasm and chloroplasts. Correlation analysis showed that RNA recognition motif (RRM) domain-containing protein (17.1 kDa) had a negative relationship with the sedimentation rate of peanut milk and that 22.0 kDa class IV heat shock protein was negatively correlated with the creaming index (p < 0.05). Bioinformatic analysis showed that the molecular function of RRM domain-containing protein (17.1 kDa) was associated with RNA binding and nucleotide binding, and 22.0 kDa class IV heat shock protein was involved in the pathway of protein processing in the endoplasmic reticulum.

Conclusion: Overall, the differentially abundant proteins in the biological metabolic pathway might offer some potential markers to guide future peanut breeding, especially for the production of peanut milk. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jsfa.11313DOI Listing
May 2021

Controlling nutritional status score as a prognostic marker to predict overall survival in resected biliary tract cancers.

Ann Transl Med 2021 Apr;9(8):644

Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences, Beijing, China.

Background: The aim of our study was to explore the prognostic significance of the preoperative controlling nutritional status (CONUT) score and establish a nomogram to predict overall survival (OS) and to achieve a more accurate prognostic risk stratification.

Methods: Clinicopathological records of 371 patients who underwent surgical resection for biliary tract cancers (BTC) from December 2002 to December 2017 were reviewed retrospectively. The associations of the CONUT score with clinicopathological factors and OS were evaluated. Univariate and multivariable Cox regression analysis were used to screen out independent predictors. A nomogram was developed and validated to estimate OS.

Results: The CONUT score was an independent predictor of OS [hazard ratio 1.478, 95% confidence interval (CI), 1.078-2.025, P=0.015]. And patients with a high CONUT score tended to have a poor prognosis with poor differentiation (P=0.011) of tumor cells and longer hospital stays (P=0.046). Besides the CONUT score, carbohydrate antigen 19-9, surgical method, and the American Joint Committee on Cancer (AJCC; 7th edition) TNM stage were contained in the final prognostic model. An OS nomogram was generated to visually predict 1-, 3-, and 5-year OS. The C-index was 0.714 (95% CI, 0.673-0.755) and 0.679 (95% CI, 0.616-0.742) in the development and validation cohort respectively. The nomogram provided superior discriminative power than the AJCC TNM staging system. The nomogram also demonstrated good risk stratification power in the entire cohort of BTC patients as well as for both BTC and surgical method subgroups.

Conclusions: The nomogram based on the CONUT score can predict OS in patients with BTCs, and it performed better than the AJCC TNM staging system.
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http://dx.doi.org/10.21037/atm-20-6770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106080PMC
April 2021

The effect of dipeptidyl peptidase IV on disease-associated microglia phenotypic transformation in epilepsy.

J Neuroinflammation 2021 May 11;18(1):112. Epub 2021 May 11.

Department of Pathophysiology, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan, 430071, China.

Background: Accumulating evidence suggests that disease-associated microglia (DAM), a recently discovered subset of microglia, plays a protective role in neurological diseases. Targeting DAM phenotypic transformation may provide new therapeutic options. However, the relationship between DAM and epilepsy remains unknown.

Methods: Analysis of public RNA-sequencing data revealed predisposing factors (such as dipeptidyl peptidase IV; DPP4) for epilepsy related to DAM conversion. Anti-epileptic effect was assessed by electroencephalogram recordings and immunohistochemistry in a kainic acid (KA)-induced mouse model of epilepsy. The phenotype, morphology and function of microglia were assessed by qPCR, western blotting and microscopic imaging.

Results: Our results demonstrated that DPP4 participated in DAM conversion and epilepsy. The treatment of sitagliptin (a DPP4 inhibitor) attenuated KA-induced epilepsy and promoted the expression of DAM markers (Itgax and Axl) in both mouse epilepsy model in vivo and microglial inflammatory model in vitro. With sitagliptin treatment, microglial cells did not display an inflammatory activation state (enlarged cell bodies). Furthermore, these microglia exhibited complicated intersections, longer processes and wider coverage of parenchyma. In addition, sitagliptin reduced the activation of NF-κB signaling pathway and inhibited the expression of iNOS, IL-1β, IL-6 and the proinflammatory DAM subset gene CD44.

Conclusion: The present results highlight that the DPP4 inhibitor sitagliptin can attenuate epilepsy and promote DAM phenotypic transformation. These DAM exhibit unique morphological features, greater migration ability and better surveillance capability. The possible underlying mechanism is that sitagliptin can reduce the activation of NF-κB signaling pathway and suppress the inflammatory response mediated by microglia. Thus, we propose DPP4 may act as an attractive direction for DAM research and a potential therapeutic target for epilepsy.
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http://dx.doi.org/10.1186/s12974-021-02133-yDOI Listing
May 2021

Trends in cause-related comorbidities in hospitalized patients with secondary hypertension in China from 2013 to 2016: a retrospective analysis of hospital quality monitoring system data.

J Hypertens 2021 May 10. Epub 2021 May 10.

Department of Cardiology, Peking University First Hospital, Beijing People's Hospital of Xinjiang Uygur Autonomous Region, Xinjiang Peking University People's Hospital, Beijing The First Affiliated Hospital of Fujian Medical University, Fujian First Hospital of Shanxi Medical University, Shanxi Huashan Hospital, Fudan University, Shanghai The First Affiliated Hospital with Nanjing Medical University, Jiangsu Scientific Project department, China Standard Medical Information Research Center, Shenzhen Clinical Trial Unit, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou Bureau of Medical Administration National Health Commission of the People's Republic of China, Beijing Tibet Autonomous Region People's Hospital, Tibet, China.

Background: Secondary hypertension has emerged as a major public health problem in China. Early diagnosis and treatment can significantly improve the clinical outcomes. However, data on the current cause composition in China are seldom reported.

Objective: To describe the trends in cause-related comorbidities in hospitalized patients with secondary hypertension in China from 2013 to 2016.

Methods: This was a retrospective analysis based on the national Hospital Quality Monitoring System (HQMS) database, which collects information from the front pages of in-hospital medical records. Hospitalized patients with secondary hypertension from 746 tertiary hospitals that consistently uploaded data to the HQMS from 2013 to 2016 were enrolled. All diagnoses were identified using International Classification of Diseases version 10 (ICD-10) diagnostic codes. Descriptive analyses were used to determine the proportions of secondary hypertension causes and changing trends over 4 years.

Result: The study collected data on 402 371 hospitalized patients with secondary hypertension from the HQMS during 2013-2016. Secondary hypertension caused by renal parenchymal disease ranked first and accounted for more than 50%. Obstructive sleep apnea syndrome (OSAS) followed closely with a rate of approximately 25%. Primary aldosteronism presented the highest proportion among all causes of endocrine hypertension. Regarding longitudinal changes over time, the rates of renal hypertension showed a significant downward trend from 2013 to 2016 (P < 0.001). In contrast, OSAS, endocrine hypertension, renal vascular disease, and aorta diseases maintained a significant upward trend from 2013 to 2016 (P < 0.001). The rates of these diseases in women with common secondary hypertension was higher than that of men, except in patients with OSAS (P < 0.001). In addition, renal parenchymal diseases and renal vascular diseases gradually decreased with age, whereas OSAS and aortic diseases gradually increased with age. The proportion of endocrine hypertension in the middle-aged group was higher than the other two age groups.

Conclusion: The study provides important information on the changing trends of cause rate of secondary hypertension modified by age and sex in China during 2013-2016. Renal parenchymal disease is still the most common cause of secondary hypertension with a decreasing trend, followed by OSAS with an increasing trend.
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http://dx.doi.org/10.1097/HJH.0000000000002891DOI Listing
May 2021

Synthesis and activity of miconazole derivatives as dual BChE/IDO1 inhibitors for the treatment of Alzheimer's disease.

Future Med Chem 2021 May 7. Epub 2021 May 7.

School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.

Alzheimer's disease is a multifactorial neurological disorder seen in elderly people. Loss of cholinergic transmission and unbalanced tryptophan metabolism kynurenine pathway have been demonstrated in neuropsychiatric diseases. Among the two series of synthesized compounds, compounds and were identified as effective dual BChE/IDO1 inhibitors, with well-balanced micromolar activity. Compounds and exhibited promising ability to ameliorate behavioral impairment by Morris water maze. The safety of miconazole analogs was also validated by PC12 and SH-SY5Y cell lines. These results highlight the ability of and to treat Alzheimer's disease.
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http://dx.doi.org/10.4155/fmc-2021-0039DOI Listing
May 2021

Immune response induced by oral administration with a Saccharomyces cerevisiae-based SARS-CoV-2 vaccine in mice.

Microb Cell Fact 2021 May 5;20(1):95. Epub 2021 May 5.

College of Medicine, Southwest Jiaotong University, Chengdu, 610031, Sichuan, China.

Background: The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlights the need to develop safe and effective vaccines with a top priority. Multiple vaccine candidates are under development, and several vaccines are currently available. Efforts need to be undertaken to counter the threat of the global COVID-19 pandemic.

Results: We generated a Saccharomyces cerevisiae (S. cerevisiae)-based SARS-CoV-2 vaccine, EBY100/pYD1-RBD, in which the full-length receptor binding domain (RBD) of the spike protein of SARS-CoV-2 was expressed on the surface of yeast. Mice vaccinated orally with unadjuvanted EBY100/pYD1-RBD could produce significant humoral and mucosal responses as well as robust cellular immune responses. Notably, EBY100/pYD1-RBD elicited a mixed Th1/Th2-type cellular immune response with a Th1-biased immune response in a mouse model.

Conclusions: Our findings highlight the importance of the RBD as a key target to design and develop vaccines against SARS-CoV-2 and provide evidence of oral administration of a S. cerevisiae-based SARS-CoV-2 vaccine eliciting significant immune responses. Most importantly, the S. cerevisiae surface display system can serve as a universal technology platform and be applied to develop other oral viral or bacterial vaccines.
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http://dx.doi.org/10.1186/s12934-021-01584-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097247PMC
May 2021

Comment on"Preoperative Single-Dose Methylprednisolone Prevents Surgical Site Infections After Major Liver Resection: A Randomized Controlled Trial.": Preoperative single-dose methylprednisolone prevents surgical site infections or protects the hepatic function.

Ann Surg 2021 Apr 30. Epub 2021 Apr 30.

Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College.

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http://dx.doi.org/10.1097/SLA.0000000000004926DOI Listing
April 2021

Enhanced formation of dichloroacetamide and dichloroacetonitrile during chloramination of drinking water and model organic matters in the presence of copper corrosion products.

Sci Total Environ 2021 Apr 22;785:147242. Epub 2021 Apr 22.

Petrochina North China Gas Marketing Company, Beijing 100029, PR China.

The formation of nitrogenous disinfection byproducts (N-DBPs) occurs in chloraminated water in drinking water distribution systems and may be affected by metal pipe materials and their corrosion products. The effect of copper corrosion products, including Cu, CuO, and CuO, on the formation of dichloroacetonitrile (DCAN) and dichloroacetamide (DCAcAm) was investigated during chloramination of natural organic matter (NOM), model precursors (carboxylic acids and amino acids), and real water samples. Copper corrosion products enhanced DCAN and DCAcAm formation during chloramination of NOM by 33%-72% and 11%-80%, respectively. Addition of N-labeled monochloramine showed that the copper corrosion products primarily enhanced the formation of DCAN using organic nitrogen and monochloramine as nitrogen sources, and the formation of DCAcAm using monochloramine as the nitrogen source, but had a limited impact on the formation of DCAcAm using organic nitrogen as the nitrogen source. A distinct N-DBP formation pathway in the presence of Cu and CuO was observed using tyrosine as a model compound, which included the formation of 1,4-benzoquinone as a dominant intermediate. On reaction with monochloramine, the 1,4-benzoquinone greatly contributed to enhancement of DCAN and DCAcAm formation using monochloramine as the nitrogen source. During chloramination of real water samples, Cu and CuO enhanced DCAN formation by 9-40% and DCAcAm formation by 16-33%. This study increases our knowledge of copper catalyzed DCAN and DCAcAm formation in copper pipes, which will be meaningful for water safety in distribution systems using chloramine disinfection.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147242DOI Listing
April 2021

Detailed chemical characterization of the composition and variability of soil gas at remediated residential heating oil discharges.

J Hazard Mater 2021 Jul 17;413:125372. Epub 2021 Feb 17.

The Charles E. Via, Jr. Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA 24061-0105, United States. Electronic address:

Underground storage tanks containing petroleum or other hazardous substances are used widely for residential storage of home heating oil. Spills and leaks of fuel from these tanks are common, and resulting subsurface petroleum vapors may pose health risks. However, understanding of this risk is limited by a lack of observational data on the chemical composition of vapors from discharged fuel. We present here the composition of soil gas sampled at 66 remediated residential sites of underground heating oil discharges throughout Virginia using a newly developed data analysis technique that allows characterization of hydrocarbons by carbon number and degree of unsaturation. Measured concentrations of total petroleum hydrocarbons exceeded 100,000 μg/m at 12 sites, but its composition varied widely between sites. Concentrations of hydrocarbons from chemical classes differing by more than a few carbon numbers or degrees of unsaturation are found to be poorly correlated. Furthermore, differences in composition are poorly described by metrics expected to indicate subsurface weathering (e.g., discharge year, or ratio of n-heptadecane to pristane). These results suggest that the composition and magnitude of residual contamination at remediated subsurface discharges is driven by rarely documented spill characteristics (e.g., age and composition of source material, discharge rate, etc.).
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http://dx.doi.org/10.1016/j.jhazmat.2021.125372DOI Listing
July 2021

Controllable High-Performance Memristors Based on 2D FeGeTeOxide for Biological Synapse Imitation.

Nanotechnology 2021 Apr 30. Epub 2021 Apr 30.

Department of Information Science and Electronic Engineering, Zhejiang University, Hangzhou, Zhejiang, CHINA.

Memristors are an important component of the next-generation artificial neural network, high computing systems, etc. In the past, two-dimensional materials based memristors have achieved a high performance and low power consumption, though one at the cost of the other. Furthermore, their performance can't be modulated frequently once their structures are fixed, which remains the bottleneck in the development. Herein, a series of forming free memristors are fabricated with the same Cu/FeGeTeoxide/FeGeTe/Al structure, yet the On/Off ratio and set voltage is modulated continuously by varying the oxidation time during fabrication. With an optimal oxidation time, a large On/Off ratio (1.58×10) and low set voltage (0.74 V) is achieved in a single device. The formation and rapture of Al conductive filaments are found to be responsible for the memristors, and the filaments density and the cross-section area increase with the increase of current compliance, which achieves a higher On/Off ratio. The memristor can imitate basic biological synaptic functions using voltage pulses, demonstrating the potential for low-power consuming neuromorphic computing applications.
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http://dx.doi.org/10.1088/1361-6528/abfd58DOI Listing
April 2021

Effects of Dietary Protein Level on the Gut Microbiome and Nutrient Metabolism in Tilapia ().

Animals (Basel) 2021 Apr 5;11(4). Epub 2021 Apr 5.

Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.

Dietary protein is one of the most important nutritional factors in aquaculture. The aim of this study was to examine the effects of dietary protein levels on the gut microbiome and the liver and serum levels of metabolites in tilapia. Tilapia were fed a diet with a low (20%), moderate (30%), or high (40%) content of crude protein, and the homeostasis of the gut microbiome and metabolic profile of the liver and serum were analyzed. The results showed no significant differences in the diversity and richness of the gut microbiome among the groups; however, there were differences in the microbial composition of the gut. The metabolome analysis of liver samples revealed a difference in the glucose level among the groups, with the highest glucose level in fish fed a high protein diet. In addition, there were significant differences in the levels of tyrosine, guanosine, and inosine among the metabolome analysis of serum samples of these groups. In summary, diets with different protein levels could affect the composition of gut microbiota and the dynamic balance of microbial communities. Dietary protein content can also affect glycolysis and amino acid metabolism in tilapia.
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http://dx.doi.org/10.3390/ani11041024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066363PMC
April 2021

High immune efficacy against different avian influenza H5N1 viruses due to oral administration of a Saccharomyces cerevisiae-based vaccine in chickens.

Sci Rep 2021 Apr 26;11(1):8977. Epub 2021 Apr 26.

College of Medicine, Southwest Jiaotong University, Chengdu, Sichuan, China.

A safe and effective vaccine is the best way to control large-scale highly pathogenic avian influenza virus (HPAI) A (H5N1) outbreaks. Saccharomyces cerevisiae (S. cerevisiae) is an ideal mucosal delivery vector for vaccine development, and we have previously shown that conventional administration of a S. cerevisiae-based vaccine (EBY100/pYD1-HA) via injection led to protection against the homologous H5N1 virus in a mouse model. Because the diameter of S. cerevisiae is approximately 10 μm, which results in a severe inflammation by injection route, therefore, oral administration is a more suitable approach for EBY100/pYD1-HA conferring protection in poultry. We extended our work by evaluating the immunogenicity and protective efficacy of oral vaccination with EBY100/pYD1-HA in the chicken model. Oral immunization with EBY100/pYD1-HA could induce robust serum IgG, mucosal IgA and cellular immune responses. Importantly, EBY100/pYD1-HA provided protection against challenges with a homologous and a heterologous H5N1 viruses. These findings suggest that EBY100/pYD1-HA, a promising H5N1 oral vaccine candidate, can avoid potential reassortment of other avian influenza viruses in oral administration of live virus vaccines and overcome the limitations of conventional injection routes. Importantly, this platform will be able to provide opportunities for broader applications in poultry during HPAI A (H5N1) outbreaks.
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http://dx.doi.org/10.1038/s41598-021-88413-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076243PMC
April 2021

COVID-19 protective measures prevent the spread of respiratory and intestinal infectious diseases but not sexually transmitted and bloodborne diseases.

J Infect 2021 Apr 22. Epub 2021 Apr 22.

College of Systems Engineering, National University of Defense Technology, Changsha 410073, Hunan, China; Department of Public Health Sciences, Karolinska Institutet, Stockholm, Sweden. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2021.04.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061184PMC
April 2021

Rapid Detection of by Real-time Recombinase-aided Amplification.

Biomed Environ Sci 2021 Apr;34(4):309-313

Centers for Disease Control and Prevention of PLA, Beijing 100071, China.

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http://dx.doi.org/10.3967/bes2021.040DOI Listing
April 2021

Anti-androgen therapy induces transcriptomic reprogramming in metastatic castration-resistant prostate cancer in a murine model.

Biochim Biophys Acta Mol Basis Dis 2021 Jul 21;1867(7):166151. Epub 2021 Apr 21.

Department of Biological Sciences, Boler-Parseghian Center for Rare and Neglected Diseases, Harper Cancer Research Institute, University of Notre Dame, Notre Dame, IN 46556, USA; Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Indianapolis, IN 46202, USA. Electronic address:

Despite recent development of next-generation androgen receptor (AR) antagonists, metastatic castration-resistant prostate cancer (CRPC) remains incurable and requires deeper understanding through studies in suitable animal models. Prostate-specific deletion of Pten and Smad4 in mice recapitulated the disease progression of human prostate adenocarcinoma, including metastasis to lymph nodes and lung. Moreover, Pten/Smad4 tumors fostered an immunosuppressive microenvironment dominated by myeloid-derived suppressor cells (MDSCs). However, the response of Pten/Smad4 tumors to androgen deprivation and anti-androgen therapies has not been described. Here, we report that the combination of surgical castration and enzalutamide treatment in Pten/Smad4 mice slowed down the tumor growth and prolonged the median survival of the mice for 8 weeks. Treatment-naïve and castration-resistant primary tumors exhibited comparable levels of immune infiltrations with the exception of reduced monocytic MDSCs in CRPC. RNA profiling of treatment-naïve and castration-resistant primary tumors revealed largely preserved transcriptome with modest expressional alterations of collagen-related and immune-related genes, among which CC chemokine receptor type 2 (Ccr2) downregulation and predicted negative activation in CRPC was consistent with reduced monocytic MDSC infiltration. Importantly, significant transcriptomic reprograming was observed in lung metastatic CRPC compared with primary CRPC and enriched for immune-related and coagulation-related pathways. At the individual gene level, we validated the expression changes of some of the most upregulated (Cd36, Bmp5, Bmp6, Etv5, Prex2, Ptprb, Egfl6, Itga8 and Cxcl12) and downregulated genes (Cxcl9 and Adamts5). Together, this study uncovers the inherent activity of Pten/Smad4 tumors to progress to CRPC and highlights potentially targetable transcriptomic signatures associated with CRPC metastasis.
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http://dx.doi.org/10.1016/j.bbadis.2021.166151DOI Listing
July 2021

Shading Promoted Theanine Biosynthesis in the Roots and Allocation in the Shoots of the Tea Plant ( L.) Cultivar Shuchazao.

J Agric Food Chem 2021 Apr 16;69(16):4795-4803. Epub 2021 Apr 16.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, Anhui 230036, China.

Shading was thought as an effective approach to increase theanine in harvested tea shoots. Previous studies offered conflicting findings, perhaps since the integration of theanine metabolism and transport in different tissues was not considered. Theanine is synthesized primarily in the roots and is then transported, via the vascular system, to new vegetative tissues. Here, we found that theanine increased in the stem, was reduced in the leaf, and remained stable in the roots, under shading conditions. Notably, in tea roots, shading significantly increased ethylamine and activated the theanine biosynthesis pathway and theanine transporter genes. Furthermore, shading significantly increased the expression of theanine transporter genes, , in the stem, while decreasing the expression of in the leaf, in accordance with shading effects on theanine levels in these tissues. These findings reveal that shading of tea plants promotes theanine biosynthesis and allocation in different tissues, processes which appear to involve the theanine biosynthesis pathway enzymes and AAP family of theanine transporters.
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http://dx.doi.org/10.1021/acs.jafc.1c00641DOI Listing
April 2021

A new method for quantification and 3D visualization of brain tumor adhesion using slip interface imaging in patients with meningiomas.

Eur Radiol 2021 Apr 14. Epub 2021 Apr 14.

Department of Radiology, Mayo Clinic, 200 First St SW, Opus 2-149, Rochester, MN, 55905, USA.

Objectives: To develop an objective quantitative method to characterize and visualize meningioma-brain adhesion using MR elastography (MRE)-based slip interface imaging (SII).

Methods: This retrospective study included 47 meningiomas (training dataset: n = 35; testing dataset: n = 12) with MRE/SII examinations. Normalized octahedral shear strain (NOSS) values were calculated from the acquired MRE displacement data. The change in NOSS at the tumor boundary (ΔNOSS) was computed, from which a 3D ΔNOSS map of the tumor surface was created and the probability distribution of ΔNOSS over the entire tumor surface was calculated. Statistical features were calculated from the probability histogram. After eliminating highly correlated features, the capability of the remaining feature for tumor adhesion classification was assessed using a one-way ANOVA and ROC analysis.

Results: The magnitude and location of the tumor adhesion can be visualized by the reconstructed 3D ΔNOSS surface map. The entropy of the ΔNOSS histogram was significantly different between adherent tumors and partially/completely non-adherent tumors in both the training (AUC: 0.971) and testing datasets (AUC: 0.900). Based on the cutoff values obtained from the training set, the ΔNOSS entropy in the testing dataset yielded an accuracy of 0.83 for distinguishing adherent versus partially/non-adherent tumors, and 0.67 for distinguishing non-adherent versus completely/partially adherent tumors.

Conclusions: SII-derived ΔNOSS values are useful for quantification and classification of meningioma-brain adhesion. The reconstructed 3D ΔNOSS surface map presents the state and location of tumor adhesion in a "clinician-friendly" manner, and can identify meningiomas with a high risk of adhesion to adjacent brain parenchyma.

Key Points: • MR elastography (MRE)-based slip interface imaging shows promise as an objective tool to preoperatively discriminate meningiomas with a high risk of intraoperative adhesion. • Measurement of the change of shear strain at meningioma boundaries can provide quantitative metrics depicting the state of adhesion at the tumor-brain interface. • The surface map of tumor adhesion shows promise in assisting precise adhesion localization, using a comprehensible, "clinician-friendly" 3D visualization.
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http://dx.doi.org/10.1007/s00330-021-07918-6DOI Listing
April 2021

Anti-Adhesion Effects of Lactobacillus Strains on Caco-2 Cells Against Escherichia Coli and Their Application in Ameliorating the Symptoms of Dextran Sulfate Sodium-Induced Colitis in Mice.

Probiotics Antimicrob Proteins 2021 Apr 14. Epub 2021 Apr 14.

College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.

The beneficial effects of probiotics on ameliorating ulcerative colitis (UC) have attracted much attention in recent years. Nevertheless, the number of these identified probiotics is still limited. In addition, the adhesion abilities of probiotics are considered to be a key determinant for probiotic efficacy. However, the relationship between the adhesion abilities of probiotics and their role in ameliorating UC has been poorly studied to date. This study measured the adhesion abilities of four Lactobacillus strains to Caco-2 cells and their anti-adhesion effects on Caco-2 cells against pathogenic bacteria, as well as their application in ameliorating the symptoms of dextran sulfate sodium-induced UC, and further illustrated the relationship between these two potential probiotic properties of probiotics and their beneficial effects on UC. Results suggested that the adhesion abilities of the four tested Lactobacillus strains exists highly strain-specific and the mechanisms of their anti-adhesion effect on Caco-2 cells against Escherichia coli may be different. Moreover, all these strains had promising effects on ameliorating UC by reducing inflammatory response and improving the intestinal mucosal barrier function, as well as promoting the production of SCFAs. In conclusion, the four tested Lactobacillus strains can be considered as alternative dietary supplements in alleviating UC. In addition, it could be concluded that there is no significant correlation between the adhesion abilities of probiotics and their role in ameliorating UC, which further illustrated that the adhesion properties of probiotics in vitro may not be suitable as the key criterion for screening potential strains with UC-alleviating effects.
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http://dx.doi.org/10.1007/s12602-021-09774-8DOI Listing
April 2021

Aberrantly Expressed Galectin-9 Is Involved in the Immunopathogenesis of Anti-MDA5-Positive Dermatomyositis-Associated Interstitial Lung Disease.

Front Cell Dev Biol 2021 25;9:628128. Epub 2021 Mar 25.

Department of Rheumatology, Beijing Key Lab for Immune-Mediated Inflammatory Diseases, China-Japan Friendship Hospital, Beijing, China.

Background: Dermatomyositis (DM) associated rapidly progressive interstitial lung disease (RP-ILD) has high mortality rate and poor prognosis. Galectin-9 (Gal-9) plays multiple functions in immune regulation. We investigated Gal-9 expression in DM patients and its association with DM-ILD.

Methods: A total of 154 idiopathic inflammatory myopathy patients and 30 healthy controls were enrolled in the study. Cross-sectional and longitudinal studies were used to analyze the association between serum Gal-9 levels and clinical features. Enzyme-linked immunosorbent assay and qRT-PCR were used to examine Gal-9 expression in the sera and isolated peripheral blood mononuclear cells (PBMCs) from DM patients. Immunohistochemistry was performed to analyze the expression of Gal-9 and its ligand (T-cell immunoglobulin mucin (Tim)-3 and CD44) in lung tissues from anti-melanoma differentiation-associated gene 5 (MDA5)-positive patients. The effect of Gal-9 on human lung fibroblasts (MRC-5) was investigated .

Results: Serum Gal-9 levels were significantly higher in DM patients than in immune-mediated necrotizing myopathy patients and healthy controls (all < 0.001). Higher serum Gal-9 levels were observed in anti-MDA5-positive DM patients than in anti-MDA5-negative DM patients [33.8 (21.9-44.7) vs. 16.2 (10.0-26.9) ng/mL, < 0.001]. Among the anti-MDA5-positive DM patients, serum Gal-9 levels were associated with RP-ILD severity. Serum Gal-9 levels were significantly correlated with disease activity in anti-MDA5-positive DM patients in both cross-sectional and longitudinal studies. PBMCs isolated from anti-MDA5-positive DM patients (3.7 ± 2.3 ng/mL) produced higher levels of Gal-9 than those from immune-mediated necrotizing myopathy patients (1.1 ± 0.3 ng/mL, = 0.022) and healthy controls (1.4 ± 1.2 ng/mL, = 0.045). The mRNA levels of Gal-9 were positively correlated with the levels of type-I interferon-inducible genes MX1 ( = 0.659, = 0.020) and IFIH1 ( = 0.787, = 0.002) in PBMCs from anti-MDA5-positive DM patients. Immunohistochemistry revealed increased Gal-9 and Tim-3 expression in the lung tissues of patients with DM and RP-ILD. stimulation with Gal-9 protein increased CCL2 mRNA expression in MRC-5 fibroblasts.

Conclusions: Among anti-MDA5-positive DM patients, Gal-9 could be a promising biomarker for monitoring disease activity, particularly for RP-ILD severity. Aberrant expression of the Gal-9/Tim-3 axis may be involved in the immunopathogenesis of DM-ILD.
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http://dx.doi.org/10.3389/fcell.2021.628128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027128PMC
March 2021

The Efficacy of Tocilizumab in the Treatment of Patients with Refractory Immune-Mediated Necrotizing Myopathies: An Open-Label Pilot Study.

Front Pharmacol 2021 16;12:635654. Epub 2021 Mar 16.

Department of Rheumatology, China-Japan Friendship Hospital, Beijing, China.

To evaluate the efficacy of tocilizumab (TCZ) in adult patients with refractory immune-mediated necrotizing myopathies (IMNMs) and investigate possible predictive biomarkers of the response to treatment with TCZ. Patients with refractory IMNM were enrolled in this open-label pilot observational study and received intravenous TCZ treatment. The clinical response was assessed after 6 months of TCZ treatment according to the 2016 American College of Rheumatology-European League Against Rheumatism (ACR-EULAR) response criteria for adult dermatomyositis and polymyositis. Muscle biopsies were performed to investigate muscle fiber regeneration by immunohistochemical staining of CD56. Serum levels of interleukin (IL)-6 were measured using a multiplex bead-based flow fluorescent immunoassay. The levels of muscle IL-6 mRNA were detected by real-time polymerase chain reaction. A total of 11 patients with refractory IMNM were enrolled in the study, including 3 anti-3-hydroxy-3-methylglutaryl-CoA reductase- and 8 anti-signal recognition particle-positive patients. Seven (63.6%) of these patients achieved clinically significant responses according to the 2016 ACR-EULAR myositis response criteria. Responders had higher baseline serum IL-6 and muscle IL-6 mRNA levels and percentage of CD56-positive muscle fibers than non-responders. Baseline serum IL-6 levels and the percentage of CD56-positive muscle fibers were positively correlated with total improvement score after 6 months of TCZ treatment. Furthermore, muscle fiber necrosis and muscle fiber size variation decreased in repeated muscle biopsies in five responders. Patients with refractory IMNM may respond to TCZ. Baseline serum IL-6 and muscle IL-6 mRNA levels and the percentage of CD56-positive muscle fibers may predict the response to TCZ treatment in these patients.
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http://dx.doi.org/10.3389/fphar.2021.635654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010666PMC
March 2021

Functions of Dendritic Cells and Its Association with Intestinal Diseases.

Cells 2021 Mar 6;10(3). Epub 2021 Mar 6.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.

Dendritic cells (DCs), including conventional DCs (cDCs) and plasmacytoid DCs (pDCs), serve as the sentinel cells of the immune system and are responsible for presenting antigen information. Moreover, the role of DCs derived from monocytes (moDCs) in the development of inflammation has been emphasized. Several studies have shown that the function of DCs can be influenced by gut microbes including gut bacteria and viruses. Abnormal changes/reactions in intestinal DCs are potentially associated with diseases such as inflammatory bowel disease (IBD) and intestinal tumors, allowing DCs to be a new target for the treatment of these diseases. In this review, we summarized the physiological functions of DCs in the intestinal micro-environment, their regulatory relationship with intestinal microorganisms and their regulatory mechanism in intestinal diseases.
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http://dx.doi.org/10.3390/cells10030583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999753PMC
March 2021

Transcriptome and lipidome profile of human mesenchymal stem cells with reduced senescence and increased trilineage differentiation ability upon drug treatment.

Aging (Albany NY) 2021 Mar 26;13(7):9991-10014. Epub 2021 Mar 26.

School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.

Human Mesenchymal stem cells (hMSCs) are multi-potential cells which are widely used in cell therapy. However, the frequently emerged senescence and decrease of differentiation capabilities limited the broad applications of MSC. Several strategies such as small molecules treatment have been widely studied and used to improve the stem characteristics bypassing the senescence but the exact mechanisms for them to reduce senescence have not been fully studied. In this study, hMSCs were treated by rapamycin, oltipraz, metformin, and vitamin C for the indicated time and these cells were subjected to senescence evaluation and trilineage differentiation. Furthermore, transcriptomics and lipidomics datasets of hMSCs after drug treatment were analyzed to interpret biological pathways responsible for their anti-senescence effects. Although four drugs exhibited significant activities in promoting MSC osteogenic differentiation, metformin is the optimal drug to promote trilineage differentiation. GO terms illustrated that the anti-aging effects of drugs were mainly associated with cellular senescence, mitotic and meiosis process. Biosynthesis of phosphatidylcholines (PC) and phosphatidylethanolamine (PE) were inhibited whereas production of phosphatidylinositols (PIs) and saturated fatty acids (SFA)/ mono-unsaturated fatty acids (MUFA) conversion was activated. Medium free fatty acids (FFA) was increased in hMSCs with different anti-aging phenotypes. Therefore, we established a comprehensive method in assessing drug intervention based on the results of transcriptomics and lipidomics. The method can be used to study different biological phenotypes upon drug intervention in MSC which will extend the clinical application of hMSCs.
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http://dx.doi.org/10.18632/aging.202759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064146PMC
March 2021

Study on Damage Mechanism and Repair of Vascular Endothelial Cells Based on Ultrasound Technology.

Biomed Res Int 2021 13;2021:6680356. Epub 2021 Mar 13.

Department of Ultrasound, Pudong New Area People's Hospital Affiliated to Shanghai University of Medicine & Health Sciences, 490 Chuanhuan South Road, Pudong New Area, Shanghai, China.

Objective: To detect the endothelial function of external iliac artery in rats with different stages of atherosclerosis by high-resolution ultrasound, so as to provide experimental methodological basis for evaluating the function of vascular endothelial cells by ultrasound.

Methods: The animals were randomly divided into the control group ( = 6) and the atherosclerosis model group ( = 15). The atherosclerosis group was further divided into 4-week group, 8-week group, and 12-week group, with 5 animals in each group. After separating and grinding rat spleen, the obtained cells were cultured by density gradient centrifugation. After the cells adhered, the morphology of the cells was observed under a microscope and identified by DiI-Ac-LDL and FITC-UEA-I double staining. The activities of LDH and SOD, the contents of MDA and GSH, and the contents of NO in plasma were detected by biochemical methods.

Results: The protective effect of rosanilin on brain injury in rats with acute hypobaric hypoxia and its regulation on the expression of pAkt protein; ox-LDL inhibited the proliferation activity of EPCs in a concentration-dependent manner. The expression of KLF2 and S1PR1 in HAEC can be knocked down by small interfering RNA, and knocking down KLF2 can not only downregulate the expression of S1PR1 but also downregulate HAVEN. With the development of atherosclerosis, the endothelium-dependent relaxation function and endothelium-independent relaxation function of the control group and the atherosclerosis model at 4, 8, and 12 weeks were damaged in different degrees and gradually aggravated.

Conclusion: Atherosclerosis is a disease with both morphological and functional damage, and vascular endothelial function is damaged in the early stage with corresponding pathological changes. Ultrasound is an effective method to evaluate vascular endothelial function.
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http://dx.doi.org/10.1155/2021/6680356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984879PMC
March 2021

Blur Detection Sensitivity Increases in Children Using Orthokeratology.

Front Neurosci 2021 12;15:630844. Epub 2021 Mar 12.

School of Ophthalmology and Optometry, Affiliated Eye Hospital, State Key Laboratory of Ophthalmology, Optometry and Vision Science, Wenzhou Medical University, Wenzhou, China.

Purpose: To investigate changes in blur detection sensitivity in children using orthokeratology (Ortho-K) and explore the relationships between blur detection thresholds (BDTs) and aberrations and accommodative function.

Methods: Thirty-two children aged 8-14 years old who underwent Ortho-K treatment participated in and completed this study. Their BDTs, aberrations, and accommodative responses (ARs) were measured before and after a month of Ortho-K treatment. A two forced-choice double-staircase procedure with varying extents of blur in three images (Tumbling Es, Lena, and Street View) was used to measure the BDTs. The participants were required to judge whether the images looked blurry. The BDT of each of the images (BDT_Es, BDT_Lena, and BDT_Street) was the average value of the last three reversals. The accommodative lag was quantified by the difference between the AR and the accommodative demand (AD). Changes in the BDTs, aberrations, and accommodative lags and their relationships were analyzed.

Results: After a month of wearing Ortho-K lenses, the children's BDT_Es and BDT_Lena values decreased, the aberrations increased significantly (for all, ≤0.050), and the accommodative lag decreased to a certain extent [T(31) = 2.029, = 0.051]. Before Ortho-K treatment, higher-order aberrations (HOAs) were related to BDT_Lena ( = 0.463, = 0.008) and the accommodative lag was related to BDT_Es ( = -0.356, = -0.046). After one month, no significant correlations were found between the BDTs and aberrations or accommodative lags, as well as between the variations of them (for all, ≥ 0.069).

Conclusion: Ortho-K treatment increased the children's level of blur detection sensitivity, which may have contributed to their good visual acuity.
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http://dx.doi.org/10.3389/fnins.2021.630844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006440PMC
March 2021

An adaptive fractional stochastic resonance method based on weighted correctional signal-to-noise ratio and its application in fault feature enhancement of wind turbine.

ISA Trans 2021 Mar 12. Epub 2021 Mar 12.

School of Automation Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan 611731, PR China.

Stochastic resonance (SR) is an effective tool to enhance weak signal by utilizing noise to reach a certain synergistic effect, which has been widely studied in the field of weak signal detection. Currently, using SR to enhance the weak fault feature of wind turbine faces two challenges: First, it is difficult for SR to select the optimal system parameters, while the traditional adaptive method based on SNR needs to predict the precise frequency of the target signal. Second, the wind turbine load changes frequently, making the vibration and noise large. As a result, the traditional SR cannot effectively highlight the target fault feature by inducing a stable resonance phenomenon at the target frequency. To improve the ability of SR to enhance the weak fault feature of wind turbine under strong noise, this paper proposes an adaptive fractional SR method based on weighted correctional signal-to-noise ratio (WCSNR). Firstly, the proposed method considers the adiabatic approximation applicable condition in the SR system and combines characteristics of the expected output signal to construct the WCSNR evaluation index to quantify the system output response, so that the system can adaptively obtain optimal parameters without predicting the accurate frequency of the target signal. Then, the fractional-order theory is applied to the SR system to overcome the shortcoming that the integer-order SR cannot induce stable resonance phenomenon at the target frequency when enhancing the fault feature of wind turbine, and use WCSNR to search for the optimal fractional order to further enhance the weak fault characteristics. Simulation and engineering actual data analysis results verify the effectiveness and superiority of the proposed method in the fault feature enhancement of wind turbine. The analysis results show that compared with the traditional SR method, the method proposed in this paper can more effectively reduce the interference of background noise and accurately enhance the weak fault feature.
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http://dx.doi.org/10.1016/j.isatra.2021.03.012DOI Listing
March 2021

Mutational spectrum and precision oncology for biliary tract carcinoma.

Theranostics 2021 4;11(10):4585-4598. Epub 2021 Mar 4.

Department of Liver Surgery, Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC), Peking Union Medical College Hospital, No. 1 Shuaifuyuan, Wangfujing, Beijing 100730, China.

The genomic spectrum of biliary tract carcinoma (BTC) has been characterized and is associated with distinct anatomic and etiologic subtypes, yet limited studies have linked genomic alterations with personalized therapies in BTC patients. This study analyzed 803 patients with BTC:164 with gallbladder cancer, 475 with intrahepatic cholangiocarcinoma (ICC) and 164 with extrahepatic cholangiocarcinoma. We determined genomic alterations, mutational signatures related to etiology and histopathology and prognostic biomarkers. Personalized targeted therapies for patients harboring potentially actionable targets (PATs) were investigated. The median tumor mutation burden (TMB) was 1.23 Mut/Mb, with 4.1% of patients having hypermutated BTCs. Unlike the results obtained from the Western population, the most frequently altered cancer-related genes in our cohort included (53%), (26%), (18%), (14%) and (14%). Germline mutations occurred mostly in DNA damage repair genes. Notably, 35.8% of the ICCs harbored aristolochic acid related signatures and an elevated TMB. and mutations and amplified 7q31.2 were demonstrated to negatively affect patient prognosis. Moreover, 19 genes were proposed to be PATs in BTCs, with 25.4% of patients harboring these PATs. Forty-six patients received PAT-matched targeted therapies, achieving a 26.1% objective response rate; the median progression-free survival (PFS) was 5.0 months, with 56.8% of patients obtaining PFS benefits. Extensive genomic diversity and heterogeneity were observed among BTC patients, with contributions according to potential etiology exposures, anatomical subtypes and clinicopathological characteristics. We also demonstrated that patients with refractory BTCs who have PATs can derive considerable benefit from receiving a matched therapy, initiating further prospective clinical trials guided by molecular profiling among this aggressive cancer.
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http://dx.doi.org/10.7150/thno.56539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978308PMC
March 2021

Association of Myopia in Elementary School Students in Jiaojiang District, Taizhou City, China.

J Ophthalmol 2021 27;2021:3504538. Epub 2021 Feb 27.

Jiaojiang Education Bureau, Taizhou, Zhejiang Province, China.

Background: The aim of our study was to evaluate the prevalence of myopia in elementary school students and to assess the risk factors for myopia.

Methods: This school-based cross-sectional study was performed on students from two elementary schools in Jiaojiang, Taizhou City, China. A total of 556 students, whose age ranged from 9 to 12 years, were included. The uncorrected visual acuity and noncycloplegic refractive error tests were performed to determine the myopia. Each student was asked to fulfill the questionnaire about the possible factors associated with myopia. Multivariate logistic analyses of risk factors were conducted.

Results: The overall prevalence of myopia among those students was 63.7%, ranged from 53.4% in grade 4 to 72.5% in grade 6. Multivariate logistic analysis showed that adjusting the height of desks and chairs according to the changing height and the presence of myopia in parents were significantly associated with myopia in these students, respectively.

Conclusions: Our results showed that myopia among elementary school students was associated with environmental and hereditary factors.
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http://dx.doi.org/10.1155/2021/3504538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936896PMC
February 2021

Efficacy and safety of corticosteroids in immunocompetent patients with septic shock.

World J Emerg Med 2021 ;12(2):124-130

Emergency Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.

Background: The use of corticosteroids in septic shock has been studied for many decades but yielded conflicting results. We conducted a systematic review to evaluate the efficacy and the safety of corticosteroids in immunocompetent patients with septic shock.

Methods: Medline via PubMed, Cochrane Central Register of Controlled Trials (CENTRAL) in the Cochrane Library, and EMBASE were searched from inception to March 2020. Two reviewers independently identified randomized controlled trials (RCTs) comparing corticosteroids with a control group for immunocompetent patients with septic shock. Data were abstracted and reported following the and Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. The efficacy outcome included mortality and shock reversal. The safety outcomes were infection, gastrointestinal bleeding, and hyperglycemia.

Results: Nine RCTs with a total of 1,298 patients were included. Compared with the control group, corticosteroid group did not lower the short-term (28 or 30 days) mortality (risk ratio [] 0.95, 95% confidence interval () 0.85 to 1.06, inconsistency []=0%, trial sequential analysis [TSA]-adjusted 0.83 to 1.09, moderate-certainty evidence). Corticosteroids significantly shortened the time to shock reversal compared with the control group (mean difference [] -21.56 hours; 95% -32.95 to -10.16, =0%; TSA-adjusted -33.33 to -9.78, moderate-certainty evidence). The corticosteroid treatment was associated with an increased risk of hyperglycemia but not the infection or gastrointestinal bleeding.

Conclusions: The corticosteroid treatment is not associated with lower short- or long- term mortality compared with placebo in immunocompetent patients with septic shock. However, corticosteroids significantly shorten the time to shock reversal without increasing the risk of infection. The patient's immune status should also be considered during clinical treatment and clinical trials in future.
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http://dx.doi.org/10.5847/wjem.j.1920-8642.2021.02.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947565PMC
January 2021

Thiamine combined with vitamin C in sepsis or septic shock: a systematic review and meta-analysis.

Eur J Emerg Med 2021 Jun;28(3):189-195

Emergency Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing.

Background: Thiamine and vitamin C have been increasingly used in patients with sepsis or septic shock because of their potential for improving metabolism and reducing mortality.

Objective: We aim to determine if thiamine combined vitamin C can reduce mortality in patients with sepsis or septic shock.

Evidence Sources And Study Selection: We comprehensively searched the PubMed, Embase, Cochrane Library, and Web of Science databases from their inception dates through 1 January 2021. Literature works evaluating the efficacy of thiamine combined vitamin C in patients with sepsis or septic shock were considered.

Data Extraction And Outcome Measurements: Two reviewers extracted data and assessed study quality. A meta-analysis was performed to calculate an odds ratio (OR), 95% confidence intervals (CIs), and P values for in-hospital mortality (primary outcome). Secondary outcomes included duration of ICU stay, duration of hospital stay, duration of vasopressor use, and change in sequential organ failure assessment (SOFA) scores.

Results: Seven randomized controlled trials were identified, encompassing a total of 868 patients. There was no statistical difference between groups for in-hospital mortality (OR: 1.11; 95% CI [0.79-1.56]; P = 0.55). Other than improving SOFA score during the first 72 h after enrollment and duration of vasopressor use, we found no other significant associations.

Conclusions: Despite widespread enthusiasm for thiamine combined with vitamin C for sepsis and septic shock, we only found an association with reduced SOFA score and time of vasopressor use. There was no association with in-hospital mortality.
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http://dx.doi.org/10.1097/MEJ.0000000000000812DOI Listing
June 2021