Publications by authors named "Xin Feng"

611 Publications

Preparation and In Vitro/In Vivo Evaluation of Orally Disintegrating/Modified-Release Praziquantel Tablets.

Pharmaceutics 2021 Sep 27;13(10). Epub 2021 Sep 27.

College of Animal Science and Technology, Guangxi University, Nanning 530004, China.

This study was designed to develop orally disintegrating/sustained-release praziquantel (PZQ) tablets using the hot-melt extrusion (HME) technique and direct compression, and subsequently evaluate their release in in vitro and in vivo pharmacokinetics. For the extrusion process, hypromellose acetate succinate (HPMCAS)-LG was the carrier of pure PZQ, with a standard screw configuration used at an extrusion temperature of 140 °C and a screw rotation speed of 100 rpm. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), powder X-ray diffraction (PXRD) and Fourier-transform infrared spectroscopy (FTIR) were performed to characterize the extrudate. Orally disintegrating/sustained-release praziquantel tablets (PZQ ODSRTs) were prepared by direct compression after appropriate excipients were blended with the extrudate. The release amount was 5.10% in pH 1.0 hydrochloric acid at 2 h and over 90% in phosphoric acid buffer at 45 min, indicating the enteric-coating character of PZQ ODSRTs. Compared with the pharmacokinetics of marketed PZQ tablets (Aipuruike) in dogs, the times to peak (T), elimination half-life (t) and mean residence time (MRT) were extended in PZQ ODSRTs, and the relative bioavailability of PZQ ODSRTs was up to 184.48% of that of Aipuruike. This study suggested that PZQ ODSRTs may have potential for the clinical treatment of parasitosis.
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http://dx.doi.org/10.3390/pharmaceutics13101567DOI Listing
September 2021

Synthesis of Novel Pinocembrin Amino Acid Derivatives and Their Antiaging Effect on via the Modulating DAF-16/FOXO.

Drug Des Devel Ther 2021 5;15:4177-4193. Epub 2021 Oct 5.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, People's Republic of China.

Purpose: Pinocembrin is a dihydroflavonoid, which is widely found in several plant species. Although pinocembrin has good pharmacological activity, it has poor water solubility and low bioavailability. Therefore, we have modified the structure of pinocembrin with a combination of different amino acids to solve this problem. Moreover, the effect of the antiaging activity of them has not been explored. We aim to investigate the effect of pinocembrin and its amino acid derivatives on the aging of .

Methods: Pinocembrin was spliced with different amino acids in order to obtain their corresponding derivatives. The preliminary research of pinocembrin and its amino acid derivatives on antiaging effect was studied by using the model. Among all the compounds, the one shows the best antiaging effect was then studied on antiaging mechanism. The protective effect on nematodes under emergency conditions was explained by detecting the ROS content and sod-3p::GFP fusion protein expression in nematodes; the possible anti-aging mechanism of nematodes was determined by DAF-16 nuclear localization experiment and the survival curve of transgenic nematodes model under stress conditions.

Results: showed the best effect on increasing tolerance to thermal and oxidative stress and reduce the accumulation of lipofuscin. In the assay of , reduced intracellular ROS accumulation. Application of to the transgenic mutant TJ356 induced the translocation of the transcription factor DAF-16 from the cytosol to the nucleus, and modulated the expression of SOD-3 (downstream genes of ), which regulates longevity in . Moreover, did not prolong the lifespan of and mutants, suggesting that these genetic pathways are involved in mediating the antiaging effects of .

Conclusion: The antioxidant and antiaging properties of may involve in the DAF-16/FOXO transcription process. Pinocembrin amino acid derivatives might be a novel agent for antiaging therapy.
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http://dx.doi.org/10.2147/DDDT.S330223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502036PMC
October 2021

Effects of Torrefaction on the Lignin of Apricot Shells and Its Catalytic Conversion to Aromatics.

ACS Omega 2021 Oct 23;6(39):25742-25748. Epub 2021 Sep 23.

College of Materials Science and Engineering, Nanjing Forestry University, 210037 Nanjing, China.

Using apricot shell lignin as a raw material, the effects of torrefaction temperatures (160, 200, 240, and 280 °C) on the properties of torrefied products were studied, and the catalytic pyrolysis experiments of the torrefied lignin under the HZSM-5 catalyst were carried out. The results showed that the oxygen content in lignin was greatly reduced and the higher heating values (HHV) gradually increased, the absorption peak of oxygen-containing functional groups gradually became weaker, and the content of the β-O-4 bond gradually decreased. At 280 °C, the C/O ratio reaches the maximum value of 2.17, and the calorific value increases to 24.22 MJ/kg. The removed oxygen element is converted into oxygen-containing components in the gas (mainly CO and HO) and liquid products (mainly guaiacol phenol). After catalytic pyrolysis of torrefied lignin, it was found that with the increase of torrefaction temperature, the relative content of aromatics increased first and then decreased slightly; the aromatics reached the maximum value of 60.63% at 240 °C; acids decreased significantly; ketones, aldehydes, and furans changed little; and torrefaction played a positive role in the conversion of lignin to aromatics.
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http://dx.doi.org/10.1021/acsomega.1c04095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495837PMC
October 2021

Multimodal Medical Image Fusion Based on Multiple Latent Low-Rank Representation.

Comput Math Methods Med 2021 28;2021:1544955. Epub 2021 Sep 28.

School of Mechanical Engineering, Key Laboratory of Manufacturing Equipment Mechanism Design and Control of Chongqing, Chongqing Technology and Business University, Chongqing 400067, China.

A multimodal medical image fusion algorithm based on multiple latent low-rank representation is proposed to improve imaging quality by solving fuzzy details and enhancing the display of lesions. Firstly, the proposed method decomposes the source image repeatedly using latent low-rank representation to obtain several saliency parts and one low-rank part. Secondly, the VGG-19 network identifies the low-rank part's features and generates the weight maps. Then, the fused low-rank part can be obtained by making the Hadamard product of the weight maps and the source images. Thirdly, the fused saliency parts can be obtained by selecting the max value. Finally, the fused saliency parts and low-rank part are superimposed to obtain the fused image. Experimental results show that the proposed method is superior to the traditional multimodal medical image fusion algorithms in the subjective evaluation and objective indexes.
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http://dx.doi.org/10.1155/2021/1544955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494599PMC
September 2021

Association between Novel Glucose-Lowering Drugs and Risk of Asthma: A Network Meta-Analysis of Cardiorenal Outcome Trials.

Diabetes Res Clin Pract 2021 Oct 6:109080. Epub 2021 Oct 6.

Department of Pharmacy, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China. Electronic address:

Aims: This network meta-analysis aimed to evaluate the asthma risk associated with dipeptidyl peptidase (DPP)-4 inhibitors, glucagon-like peptide-1 receptor agonists (GLP-1RAs), and sodium-glucose co-transporter (SGLT) 2 inhibitors.

Methods: Electronic databases were systematically searched up to March 2021 to include placebo-controlled cardiovascular (or cardiorenal) outcome trials that reported the asthma incidents in patients taking DPP-4 inhibitors, GLP-1RAs, or SGLT2 inhibitors. A random-effects network meta-analysis was conducted to estimate their odds ratio (ORs) and 95% confidence intervals (CIs).

Results: Nineteen trials including 218 asthma cases among 159,705 patients were included. Compared with placebo, SGLT2 inhibitors (OR, 0.59; 95% CI, 0.38-0.93) were significantly associated with a decreased risk of asthma while both DPP-4 inhibitors and GLP-1RAs did not significantly affect asthma risk. SGLT2 inhibitors were significantly associated with a lower risk of asthma than DPP-4 inhibitors (OR, 0.38; 95% CI, 0.18-0.79). There was no association between GLP-1RAs and DPP-4 inhibitors and between SGLT2 inhibitors and GLP-1RAs in risk of asthma.

Conclusions: SGLT2 inhibitors might protect against asthma while DPP-4 inhibitors and GLP-1RAs did not significantly affect the asthma incident. Given the underreporting of asthma in this study, further investigations using real-world data as well as mechanistic studies are warranted to confirm our results.
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http://dx.doi.org/10.1016/j.diabres.2021.109080DOI Listing
October 2021

Boosting Sodium Storage Performance of Hard Carbon Anodes by Pore Architecture Engineering.

ACS Appl Mater Interfaces 2021 Oct 1;13(40):47671-47683. Epub 2021 Oct 1.

School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, PR China.

Hard carbon (HC) displays great potential for high-performance sodium-ion batteries (SIBs) due to its cost-effective, simple fabrication and most likely to be commercialized. However, the complicated microstructures of HC lead to difficulties in deeply understanding the structure-performance correlation. Particularly, evaluation of influence of pore structure on Na storage performances is still causing disputes and rational strategies of designing pore architecture of HC are still necessary. In this work, the skillful and controllable phase-inversion method is applied to construct porous HC with abundantly interconnected and permeable tunnel-like pores, which can promote ionic diffusion and improve electrode-electrolyte interfacial affinity. Structure-performance investigation reveals that porous HC with cross-coupled macropore architecture can boost Na storage performances comprehensively. Compared to pristine HC with negligible pores, well-regulated porous HC anodes show an obvious enhancement on initial Coulombic efficiency (ICE) of 68.3% (only 51.5% for pristine HC), reversible capacity of 332.7 mAh g at 0.05 A g, rate performance with 67.4% capacity retention at 2 A g (46.5% for pristine HC), and cycling stability with 95% capacity maintained for 90 cycles (86.4% for pristine HC). Additionally, the ICE can be optimized up to 76% by using sodium carboxymethyl cellulose as a binder. This work provides an important view of optimizing Na storage performances of HC anodes by pore engineering, which can be broadened into other electrode materials.
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http://dx.doi.org/10.1021/acsami.1c14738DOI Listing
October 2021

Pipeline Embolization Device for the Treatment of Unruptured Intracranial Dissecting Aneurysms.

Front Neurol 2021 14;12:691897. Epub 2021 Sep 14.

Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.

Intracranial dissecting aneurysms (IDAs) are rare but pose significant challenges to treatment. The pipeline embolization device (PED) has been demonstrated to be an effective treatment option with excellent outcomes. Herein, we report our experience with patients treated with the PED for unruptured IDAs. We retrospectively reviewed our hospital database and identified patients who were treated with PEDs for unruptured IDAs between March 2016 and September 2020. Data including demographics, clinical presentation, aneurysm characteristics, procedural details, intra- or peri-procedural complications, and follow-up details were collected. Eighty patients (61 men, 76.25%) were treated with PED for unruptured IDAs. The most common symptoms were headache (34, 42.5%), dizziness (29, 36.25%), and nausea or vomiting (15, 18.75%). Of these patients, 73 had one aneurysm, and seven harbored two aneurysms. All of them achieved successful PED deployment. Six patients experienced intra- or peri-procedural complications including perforator artery occlusion, thromboembolic, hemorrhagic events, and falling of the stent into the aneurysm sac. Follow-up with digital subtractive angiography was available for 29 patients with a median of 6 months, and 28 (96.56%) patients had aneurysm occlusion. Late thrombosis occurred in four patients, and two of them had unfavorable outcomes. Clinical follow-up showed that a favorable clinical outcome was achieved in 76 (95%) patients, and the mortality rate was 3.75%. Treating unruptured IDAs is safe and effective with long-term favorable clinical and angiographic outcomes. However, the complications of this treatment should be noted. Careful selection of appropriate patients and individualized antiplatelet therapy might be needed.
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http://dx.doi.org/10.3389/fneur.2021.691897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476866PMC
September 2021

Dynamic, bidirectional influences of children's emotions and maternal regulatory strategies.

Emotion 2021 Sep 27. Epub 2021 Sep 27.

Department of Human Sciences, The Ohio State University.

The current study examined temporal associations between child emotion and maternal regulatory strategies in mother-child dyads. One hundred and 30 mothers and their 5-year-old children (46.1% boys) completed a challenging puzzle task, which was videotaped. Child positive and negative emotion as well as mothers' regulatory strategies including problem solving, providing approval, and comforting, were coded on a second-by-second basis from the videos. Multilevel modeling analyses were conducted to examine how maternal behaviors at a given time predicted the within-dyad variations of child positive and negative emotion 2 seconds later, controlling for the child's own prior emotion, as well as child emotion predicting subsequent maternal behaviors 2 seconds later, controlling for mothers' prior behaviors. In all models, child sex and negative emotionality as well as maternal depressive symptoms were included as between-dyad predictors, whereas maternal race and education were included as covariates. Results suggested that maternal approval and problem solving predicted within-dyad variation in subsequent child positive emotion. Both child positive and negative emotion was predictive of the within-dyad variation in mothers' subsequent use of strategies. Maternal depressive symptoms moderated the relations between prior and subsequent strategy uses for all three maternal behaviors. Our findings indicated that child emotion regulates, and is regulated by, maternal regulatory strategies. (PsycInfo Database Record (c) 2021 APA, all rights reserved).
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http://dx.doi.org/10.1037/emo0001005DOI Listing
September 2021

Preparation, evaluation, and pharmacokinetics in beagle dogs of a taste-masked flunixin meglumine orally disintegrating tablet prepared using hot-melt extrusion technology and D-optimal mixture design.

Eur J Pharm Sci 2021 Sep 24;168:106019. Epub 2021 Sep 24.

College of Animal Science and Technology, Guangxi University, 100 Daxue Road, Xixiangtang District, Nanning, Guangxi 530005, China. Electronic address:

Flunixin meglumine (FM) is a nonsteroidal anti-inflammatory drug limited by irritation of the respiratory tract and mucosa in veterinary tissue. This study aimed to develop a taste-masked FM solid dispersion (SD) by hot-melt extrusion (HME) and formulate an orally disintegrating tablet (ODT) with selected excipients by direct compression. Eudragit® E PO was chosen as the matrix, and HME parameters were optimized: extrusion temperature, 135℃; screw speed, 100 rpm; and drug loading, 20%. Characterization techniques proved that FM was rendered amorphous in the HME extrudate. In vitro dissolution studies showed that FM SD released significantly slower than the corresponding physical mixture in artificial saliva. Excipients were selected based on compression formability, disintegration, and solubility. A D-optimal mixture design was used to optimize the composition: 25% FM SD, 18.75% microcrystalline cellulose, 52.5% mannitol, 3.75% low-substituted hydroxypropyl cellulose, and 1% magnesium stearate. Taste-masked FM ODT had a tensile strength of 0.7 ± 0.01 MPa and a disintegration time of 17.6 ± 0.1 s. E-tongue and E-nose analysis showed that FM ODT had a better taste-masked effect than commercial granules. Finally, a pharmacokinetic study proved that the main pharmacokinetic parameters of FM ODT were not significantly different from those of commercial granules, which indicated that these formulations had similar pharmacokinetic behaviours in beagles.
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http://dx.doi.org/10.1016/j.ejps.2021.106019DOI Listing
September 2021

NMR-based metabolomics approach to evaluate the toxicological risks of Tibetan medicine 'Ershiwuwei Shanhu' pill in rats.

J Ethnopharmacol 2022 Jan 10;282:114629. Epub 2021 Sep 10.

Beijing Hospital of Tibetan Medicine, China Tibetology Research Center, Beijing, 100029, China; Institute for Tibetan Medicine, China Tibetology Research Center, Beijing, 100101, China. Electronic address:

Ethnopharmacological Relevance: 'Ershiwuwei Shanhu' Pill (ESP) is a classic Tibetan medicine to treat neurological disorders in nervous system, especially for neurological pains and epilepsy. It contains many Tibet-specific mineral medicines, among which Halloysite (Halloysitum rubrum, HR) was regarded as the main active one. As mineral medicines contain heavy metals with poor solubility, the doubts about its safety restricted its clinical application and further development.

Aim Of The Study: A 7-day acute toxicity of ESP and its main active mineral medicine HR was systematically studied for investigating the safety of ESP and exploring the role of HR in ESP's potential toxicity.

Materials And Methods: In this study, the acute oral toxicity assessment of formula-ESP and HR were performed on rats for 7 days at doses equivalent to 10 (1 g/kg) and 40 times (4 g/kg) the typical clinical dose (0.1 g/kg). H NMR based metabolomics profiling, aided with biochemical analysis and histopathology, was conducted to explore the global metabolic changes in the livers and kidneys of the administrated rats.

Results: High-dose HR caused oxidative stress, energy metabolism disorders, purine metabolism impairments and amino acid metabolism imbalance in rats, resulting in hepatotoxicity and nephrotoxicity, which in accordance with the increased biochemical index in blood (ALT, AST, BUN and CRE). ESP (low-dose) induced metabolites changes were far more less than HR in livers, showcasing the distinct advantage of formula in reducing toxicity. Furthermore, low-dose ESP disturbed renal metabolism in a way similar to high-dose HR, which implies that HR might be the major source of the potential nephrotoxicity of ESP.

Conclusion: HR exhibited potential hepatoxicity and nephrotoxicity, but the formula- 'Ershiwuwei Shanhu' Pill which contains HR is considered relatively safe.
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http://dx.doi.org/10.1016/j.jep.2021.114629DOI Listing
January 2022

Loss of signal transducer and activator of transcription 3 impaired the osteogenesis of mesenchymal progenitor cells in vivo and in vitro.

Cell Biosci 2021 Sep 8;11(1):172. Epub 2021 Sep 8.

Department of Operative Dentistry and Endodontics, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, China.

Background: Signal transducer and activator of transcription 3 (Stat3) is a cytoplasmic transcription factor that participates in various biologic processes. Loss of Stat3 causes hyperimmunoglobulin E syndrome, presenting with skeletal disorders including osteoporosis, recurrent fractures, scoliosis, and craniosynostosis. The objective of this study is to explore the effect and mechanism of Stat3 on osteogenesis of mesenchymal progenitors.

Methods: Stat3 was conditionally knockout (CKO) in mesenchymal progenitors by crossing the pair-related homeobox gene 1-cre (Prx1-Cre) with Stat3-floxed strain mice. Whole-mount-skeletal staining, histology, and micro-CT were used to assess the differences between Stat3 CKO and control mice. Further, in vitro experiments were conducted to evaluate the osteogenesis potential of primary isolated bone marrow mesenchymal stem cells (BMSCs) from both control and Stat3 CKO mice. After osteogenic induction for 14d, alizarin red staining was used to show the calcium deposit, while the western blotting was applied to detect the expression of osteogenic markers.

Results: Compared with the control, Stat3 CKO mice were present with shortened limbs, multiple fractures of long bone, and open calvarial fontanels. The abnormal growth plate structure and reduced collagen fiber were found in Stat3 CKO limbs. According to micro-CT analysis, the reduced cortical bone thickness and bone volume were found on Stat3 CKO mice. The in vitro osteogenic differentiation of BMSCs was inhibited in Stat3 CKO samples. After osteogenic induction for 14d, the significantly diminished calcium deposits were found in Stat3 CKO BMSCs. The decreased expression of osteogenic markers (OPN and COL1A1) was observed in Stat3 CKO BMSCs, compared with the control.

Conclusions: Stat3 played a critical role in bone development and osteogenesis. Loss of Stat3 impaired the osteogenesis of mesenchymal progenitors in vivo and in vitro.
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http://dx.doi.org/10.1186/s13578-021-00685-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424822PMC
September 2021

Downregulation of Filamin a Expression in the Aorta Is Correlated With Aortic Dissection.

Front Cardiovasc Med 2021 13;8:690846. Epub 2021 Aug 13.

Division of Cardiothoracic and Vascular Surgery, Sino-Swiss Heart-Lung Transplantation Institute, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Filamins (FLNs) are actin cross-linking proteins, and as scaffolding proteins, FLNs are closely associated with the stabilization of the cytoskeleton. Nevertheless, the biological importance of FLNs in aortic dissection (AD) has not been well-elucidated. In this study, we first reanalyzed datasets downloaded from the Gene Expression Omnibus (GEO) database, and we found that in addition to the extracellular matrix, the actin cytoskeleton is a key structure associated with AD. Given that FLNs are involved in remodeling the cytoskeleton to affect cellular functions, we measured their expression levels in the aortas of patients with Stanford type A AD (TAAD). Our results showed that the mRNA and protein levels of FLNA were consistently decreased in dissected aortas of both humans and mice, while the FLNB protein level was upregulated despite decreased FLNB mRNA levels, and comparable expression levels of FLNC were observed between groups. Furthermore, the immunohistochemistry results demonstrated that FLNA was highly expressed in smooth muscle cells (SMCs) of aorta in non-AD samples, and downregulated in the medial layer of the dissected aortas of humans and mice. Moreover, we revealed that FOS and JUN, forming a dimeric transcription factor called AP-1 (activating protein-1), were positively correlated with the expression of FLNA in aorta. Either overexpression of FOS or JUN alone, or overexpression of FOS and JUN together, facilitated the expression of FLNA in primary cultured human aortic SMCs. In the present study, we not only detected the expression pattern of FLNs in aortas of humans and mice with or without AD, but we also found that the expression of FLNA in the AD samples was significantly reduced and that AP-1 might regulate the expression of FLNA. Our findings will contribute to the elucidation of the pathological mechanisms of AD and provide potential therapeutic targets for AD.
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http://dx.doi.org/10.3389/fcvm.2021.690846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414519PMC
August 2021

Outcomes of intramural hematoma involving the ascending aorta and extending into the descending thoracic aorta.

J Vasc Surg 2021 Sep 2. Epub 2021 Sep 2.

Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan, China; NHC Key Laboratory of Organ Transplantation, Wuhan, China; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China. Electronic address:

Objective: The optimal treatment of intramural hematoma (IMH) involving the ascending aorta remains controversial. This study aimed to analyze the results of the management of patients with acute IMH involving the ascending aorta and extending into the descending thoracic aorta, to compare outcomes of descending thoracic endovascular aortic repair (TEVAR) with that of medical therapy (MT), and to assess the risk factors associated with adverse aortic events.

Methods: We retrospectively analyzed all patients diagnosed with acute IMH involving the ascending aorta and extending into the descending thoracic aorta from January 2012 to December 2019. The primary end points during follow-up were aortic disease-related death and adverse aorta-related events that required surgical or endovascular treatment, such as aortic rupture, the progression of aortic disease, or endoleak.

Results: We identified a total of 135 patients with acute IMH involving the ascending aorta and extending into the descending thoracic aorta, of whom 104 underwent descending TEVAR (group 1) and 31 were managed with MT (group 2). Freedom from adverse aorta-related events at 1, 3, and 5 years was significantly higher for patients who underwent descending TEVAR compared with those managed with MT (89.2%, 88.2%, and 84.0% vs 74.2%, 74.2%, and 74.2%, respectively; P = .026). The 1-, 3-, and 5-year survival rates for patients in the descending TEVAR group was 100%, 100%, and 100%, respectively, which was significantly higher than the survival of the MT group: 93.5%, 93.5%, and 81.9%, respectively (P = .002). On a univariate analysis among patients receiving MT, those who suffered adverse aorta-related events showed a higher prevalence of renal insufficiency (55.6% vs 9.1%; P = .003). In MT patients, multivariate analysis showed that renal insufficiency was the only independent risk factor associated with adverse aorta-related events (hazard ratio, 8.691; 95% confidence interval, 2.056-36.737; P = .003).

Conclusions: Based on our study, compared with MT, descending TEVAR might be the more favorable treatment for patients with IMH involving the ascending aorta and extending into the descending thoracic aorta. Patients with renal insufficiency are more likely to experience adverse aorta-related events, which implies the need for subsequent intervention or an increased risk of mortality. The risk factor would be helpful for clinical decision-making.
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http://dx.doi.org/10.1016/j.jvs.2021.07.231DOI Listing
September 2021

Liquid chromatography-mass spectrometry in-depth analysis and in silico verification of the potential active ingredients of Baihe Dihuang decoction in vivo and in vitro.

J Sep Sci 2021 Sep 2. Epub 2021 Sep 2.

Department of Analysisand Testing, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.

Baihe Dihuang decoction is a commonly used herbal formula to treat depression and insomnia in traditional Chinese medicine. This study established a liquid chromatography-mass spectrometry method to investigate the potential active ingredients and the components absorbed in the blood and brain tissue of mice. Using a new data processing method, 94 chemical components were identified, 33 and 9 of which were absorbed in the blood and brain. More interestingly, we analyzed the substance changes during co-decoction and the characteristics of the compounds absorbed in the blood and brain. The results show that 71 newly generated chemical components were discovered from co-decoction: 38 with fragment information and five absorbed in the blood. Ultimately, the results of molecular docking show that these components have excellent performance in proteins of γ-aminobutyric acid, serotonin and melatonin receptors. The docking results of emodin with Monoamine Oxidase A and Melatonin Receptor 1A, and luteolin with Solute Carrier Family 6 Member 4, Glyoxalase I, Monoamine Oxidase B and Melatonin Receptor 1A, may explain the mechanism of action of Baihe Dihuang decoction in treating insomnia and depression. Overall, our research results may provide novel perspectives for further understanding of the effective substances in Baihe Dihuang decoction.
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http://dx.doi.org/10.1002/jssc.202100434DOI Listing
September 2021

Efficacy and safety of PARP inhibitors in patients with BRCA-mutated advanced breast cancer: A meta-analysis and systematic review.

Breast 2021 Aug 21;60:26-34. Epub 2021 Aug 21.

Department of Pharmacy, Beijing Chao-Yang Hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, China. Electronic address:

Objective: This meta-analysis aimed to investigate the efficacy and safety of poly (adenosine diphosphate-ribose) polymerase (PARP) inhibitors in BRCA-mutated advanced breast cancer patients comprehensively.

Methods: We conducted a systematic literature research through PubMed, the Cochrane Central Register of Controlled Trials (CENTRAL), Embase, China National Knowledge Infrastructure (CNKI), wanfang, China Biology Medicine disc (CBMdisc), and ClinicalTrials.gov from inception to January 2021. Randomized controlled trials (RCTs) with available data comparing PARP inhibitors versus control therapy in BRCA-mutated advanced breast cancer were eligible for analysis. Statistical analyses were performed with Review Manager (RevMan) version 5.4 and R version 4.0.3.

Results: 1706 studies were retrieved in total, and 4 RCTs with 1540 patients were eligible for meta-analysis finally. The results showed that progression-free survival (PFS) and overall survival (OS) were significantly improved in germline BRCA-mutated breast cancer patients with PARP inhibitors (HR 0.64, 95% CI [0.56-0.74]; HR 0.86, 95% CI [0.74-0.99], respectively) with no significant heterogeneity across studies (I = 22%, χ p = 0.28; I = 0%, χ p = 0.70, respectively). There was no significant difference in the overall adverse events (AEs), grade≥3 AEs and AEs leading to treatment discontinuation between PARP inhibitor arms and control arms (RR 1.01, 95% CI [0.99-1.02]; RR 0.95, 95% CI [0.83-1.09]; RR 1.17, 95% CI [0.87-1.57], respectively). Based on the available data, PARP inhibitors provided comparable or better results than control arms in improving the quality of life in BRCA-mutated advanced breast cancer patients.

Conclusions: PARP inhibitors prolonged PFS and OS among patients with BRCA-mutated advanced breast cancer with tolerable safety and improved quality of life.
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http://dx.doi.org/10.1016/j.breast.2021.08.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399371PMC
August 2021

Identification of Copy Number Variants in a Southern Chinese Cohort of Patients with Congenital Scoliosis.

Genes (Basel) 2021 Aug 5;12(8). Epub 2021 Aug 5.

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

Congenital scoliosis (CS) is a lateral curvature of the spine resulting from congenital vertebral malformations (CVMs) and affects 0.5-1/1000 live births. The copy number variant (CNV) at chromosome 16p11.2 has been implicated in CVMs and recent studies identified a compound heterozygosity of 16p11.2 microdeletion and variant/haplotype causing CS in multiple cohorts, which explains about 5-10% of the affected cases. Here, we studied the genetic etiology of CS by analyzing CNVs in a cohort of 67 patients with congenital hemivertebrae and 125 family controls. We employed both candidate gene and family-based approaches to filter CNVs called from whole exome sequencing data. This identified 12 CNVs in four scoliosis-associated genes (, , , and ) as well as eight recessive and 64 novel rare CNVs in 15 additional genes. Some candidates, such as , , , and , have been found to be associated with syndromes with scoliosis or implicated in bone/spine development. In particular, the mutant mouse showed spinal deformities. Our findings suggest that, in addition to the 16p11.2 microdeletion, other CNVs are potentially important in predisposing to CS.
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http://dx.doi.org/10.3390/genes12081213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391542PMC
August 2021

Binary Network of Conductive Elastic Polymer Constraining Nanosilicon for a High-Performance Lithium-Ion Battery.

ACS Nano 2021 Sep 25;15(9):14570-14579. Epub 2021 Aug 25.

Research Centre of Nanoscience and Nanotechnology, Shanghai University, Shanghai 200444, China.

Silicon-based anodes are attracting more interest in both science and industry due to their high energy density. However, the traditional polymeric binder and carbon additive mixture cannot successfully accommodate the huge volume change and maintain good conductivity when cycling. Herein, we report a multifunctional polymeric binder (PPTU) synthesized by the cross-linking of conducting polymer (PEDOT:PSS) and stretchable polymer poly(ether-thioureas) (PETU). The multifunctional polymeric binder could be curved on the surfaces of nanosilicon particles, forming an interweaving continuous three-dimensional network, which is beneficial to electron transfer and the mechanical stability. Furthermore, the binder is elastic and adhesive, and which can accommodate the huge volume change of silicon to keep its integrity. Utilizing this multifunctional polymeric binder instead of commercial poly(acrylic acid) binder and carbon black mixtures, the nanosilicon anode demonstrates enhanced cycling stability (2081 mAhg after 300 cycles) and rate performance (908 mAhg at 8 Ag). The multifunctional polymeric binder has high conductivity, elasticity, and self-healing properties is a promising binder to promote progress toward a high performance lithium-ion battery.
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http://dx.doi.org/10.1021/acsnano.1c04240DOI Listing
September 2021

Aerodynamic characteristics in upper airways among orthodontic patients and its association with adenoid nasopharyngeal ratios in lateral cephalograms.

BMC Med Imaging 2021 08 23;21(1):127. Epub 2021 Aug 23.

Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Årstadveien 19, 5009, Bergen, Norway.

Background: Adenoid hypertrophy among orthodontic patients may be detected in lateral cephalograms. The study investigates the aerodynamic characteristics within the upper airway (UA) by means of computational fluid dynamics (CFD) simulation. Furthermore, airflow features are compared between subgroups according to the adenoidal nasopharyngeal (AN) ratios.

Methods: This retrospective study included thirty-five patients aged 9-15 years having both lateral cephalogram and cone beam computed tomography (CBCT) imaging that covered the UA region. The cases were divided into two subgroups according to the AN ratios measured on the lateral cephalograms: Group 1 with an AN ratio < 0.6 and Group 2 with an AN ratio ≥ 0.6. Based on the CBCT images, segmented UA models were created and the aerodynamic characteristics at inspiration and expiration were simulated by the CFD method for the two groups. The studied aerodynamic parameters were pressure drop (ΔP), maximum midsagittal velocity (V), maximum wall shear stress (P), and minimum wall static pressure (P).

Results: The maximum V exhibits nearly 30% increases in Group 2 at both inspiration (p = 0.013) and expiration (p = 0.045) compared to Group 1. For the other aerodynamic parameters such as ΔP, the maximum P, and minimum P, no significant difference is found between the two groups.

Conclusions: The maximum V seems to be the most sensitive aerodynamic parameter for the groups of cases. An AN ratio of more than 0.6 measured on a lateral cephalogram may associate with a noticeably increased maximum V, which could assist clinicians in estimating the airflow features in the UA.
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http://dx.doi.org/10.1186/s12880-021-00659-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381502PMC
August 2021

Reversible Switching of Single-Molecule Magnetic Behaviour by Desorption/Adsorption of Solvent Ligand in a New Dy(III)-Based Metal Organic Framework.

Front Chem 2021 5;9:714851. Epub 2021 Aug 5.

State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.

Two metal-organic frameworks (MOFs), [Dy(BDC)(NO)(DMF)] (, HBDC = terephthalic acid) and [Dy(BDC)(NO)] (), were synthesized. The structures of MOFs and are easy to be reversibly transformed into each other by the desorption or adsorption of coordination solvent molecules. Accordingly, their magnetic properties can also be changed reversibly, which realizes our goals of manipulating on/off single-molecule magnet behaviour. MOF behaves as a single-molecule magnet either with or without DC field. Contrarily, no slow magnetic relaxation was observed in both under zero field and applied field.
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http://dx.doi.org/10.3389/fchem.2021.714851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374150PMC
August 2021

Influence of the Integrated Delivery System on the Medical Serviceability of Primary Hospitals.

J Healthc Eng 2021 2;2021:9950163. Epub 2021 Aug 2.

Department of Medicine, Huzhou University, Huzhou, Zhejiang, China.

Objective: To explore the change in the medical serviceability of primary hospitals since the establishment of the Huzhou No. 1 People's Hospital medical care group incorporating the integrated delivery system.

Methods: With reference to the "Grade Evaluation Standard of General Hospitals in Zhejiang Province" and the "Guidelines for Service Capacity Evaluation of Township Hospitals (2019 Edition)," we analyzed the influence of the integrated delivery system on the capacity of primary medical services and selected the targeted core indicators. From the four dimensions of diagnosis and treatment breadth, diagnosis and treatment efficiency, surgical ability, and patient satisfaction, an index evaluation system was established to explore the changes in the medical serviceability in primary hospitals.

Results: The measurements were aimed at four specific issues, that is, the low medical technology level of grassroots personnel, the poor information communication among medical institutions, the difficulty in recruiting people, and the imperfect training mechanism in primary hospitals. After establishing a series of measurements related to the problems faced by the primary healthcare sector in China, the score of breadth of diagnosis and treatment, efficiency of diagnosis and treatment ability, surgical ability, and patient satisfaction of the primary hospitals in our medical group have greatly increased.

Conclusion: The integrated delivery system improved the primary hospitals' medical health ability obviously. Our study also provides various useful and operable suggestions for primary healthcare.
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http://dx.doi.org/10.1155/2021/9950163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8356014PMC
August 2021

Interleukin-5 receptor alpha (CD125) expression on human blood and lung neutrophils.

Ann Allergy Asthma Immunol 2021 Aug 12. Epub 2021 Aug 12.

Department of Medicine, University of Virginia, Charlottesville, Virginia; Department of Microbiology, University of Virginia, Charlottesville, Virginia.

Background: Our previous studies revealed the presence of interleukin-5 (IL-5) receptor alpha chain (IL-5Rα, CD125) on neutrophils in a murine model of influenza and in the lung fluid of children with severe asthma.

Objective: To further evaluate the functional characteristics and effects of clinical factors and inflammatory variables on neutrophil surface IL-5Rα abundance in lung fluid and blood.

Methods: IL-5Rα expression was quantified by flow cytometry performed on purified neutrophils from blood and bronchoalveolar lavage fluid samples obtained from healthy controls and individuals with asthma. Expression was further confirmed by immunohistochemistry. Functional signaling through the IL-5Rα was evaluated by measurement of IL-5-inducible modulation of neutrophil surface CD62L and IL-5Rα expression.

Results: IL-5Rα was consistently present but at a variable magnitude on blood and lung neutrophils. Expression on lung neutrophils was significantly higher than that on blood cells (p"?>P < .001) where their expression was higher in the presence of airway pathogens, especially with respiratory viruses. Increased receptor expression occurred in response to the translocation of preformed receptors from intracellular stores. Receptors were functional as revealed by IL-5-mediated down-regulation of CD62L and the feed-forward up-regulation of reception expression.

Conclusion: In addition to the expression on eosinophils and basophils, the IL-5Rα is consistently and abundantly expressed on the surface of blood and especially air space neutrophils. These observations support the concept that some of the efficacy of IL-5/IL-5R-targeting biologics observed in asthma may reflect their ability to target neutrophilic air space inflammation.
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http://dx.doi.org/10.1016/j.anai.2021.08.004DOI Listing
August 2021

Complex Environment Path Planning for Unmanned Aerial Vehicles.

Sensors (Basel) 2021 Aug 3;21(15). Epub 2021 Aug 3.

College of Computer Science and Technology, Jilin University, Changchun 130012, China.

Flying safely in complex urban environments is a challenge for unmanned aerial vehicles because path planning in urban environments with many narrow passages and few dynamic flight obstacles is difficult. The path planning problem is decomposed into global path planning and local path adjustment in this paper. First, a branch-selected rapidly-exploring random tree (BS-RRT) algorithm is proposed to solve the global path planning problem in environments with narrow passages. A cyclic pruning algorithm is proposed to shorten the length of the planned path. Second, the GM(1,1) model is improved with optimized background value named RMGM(1,1) to predict the flight path of dynamic obstacles. Herein, the local path adjustment is made by analyzing the prediction results. BS-RRT demonstrated a faster convergence speed and higher stability in narrow passage environments when compared with RRT, RRT-Connect, P-RRT, 1-0 Bg-RRT, and RRT*. In addition, the path planned by BS-RRT through the use of the cyclic pruning algorithm was the shortest. The prediction error of RMGM(1,1) was compared with those of ECGM(1,1), PCGM(1,1), GM(1,1), MGM(1,1), and GDF. The trajectory predicted by RMGM(1,1) was closer to the actual trajectory. Finally, we use the two methods to realize path planning in urban environments.
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http://dx.doi.org/10.3390/s21155250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347258PMC
August 2021

Research Note: Effects of glycerol monolaurate supplementation on egg production, biochemical indices, and gut microbiota of broiler breeders at the late stage of production.

Poult Sci 2021 Sep 14;100(9):101386. Epub 2021 Jul 14.

School of Life Science and Engineering, Foshan University, Foshan 528000, China. Electronic address:

The objective of the present study was to determine the effect of glycerol monolaurate (GML) supplementation on egg production, biochemical indices, and gut microbiota of broiler breeders at the late stage of production. Total of 180 healthy Qingyuan Partridge broiler breeders were randomly assigned to 2 groups: 1) corn-soybean meal based diet, and 2) basal diet supplemented with 300 mg glycerol monolaurate/kg. Each treatment group had 6 replicates with 15 birds within each replicate. The experiment started at wk 33 and lasted for 8 weeks. Feed conversion rate, egg weight, egg shape index, shell breaking strength, and shell thickness were not different between control and treatment groups. Supplementation of GML significantly decreased the egg breaking rate. All blood chemical indices and antioxidant parameters were not affected by GML except total antioxidant capacity which increased significantly with GML supplementation. Alpha diversity indices (Shannon, Simpson, Chao1, Ace, goods_coverage, and PD_whole tree) were not different between the 2 groups. Composition of cecal microbiota was not affected by GML supplementation except Euryarchaeota and Proteobacteria at phylum level. Overall, supplementation of glycerol monolaurate at 300 mg/kg level improved eggshell quality but its effect on cecal microbiota composition was limited on broiler breeders at the late stage of production.
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http://dx.doi.org/10.1016/j.psj.2021.101386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350544PMC
September 2021

Association between metabolic status and gut microbiome in obese populations.

Microb Genom 2021 Aug;7(8)

Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen 518000, PR China.

Despite that obesity is associated with many metabolic diseases, a significant proportion (10-30 %) of obese individuals is recognized as 'metabolically healthy obeses' (MHOs). The aim of the current study is to characterize the gut microbiome for MHOs as compared to 'metabolically unhealthy obeses' (MUOs). We compared the gut microbiome of 172 MHO and 138 MUO individuals from Chongqing (China) (inclined to eat red meat and food with a spicy taste), and performed validation with selected biomarkers in 40 MHOs and 33 MUOs from Quanzhou (China) (inclined to eat seafood and food with a light/bland taste). The genera , and had increased abundance in both Chongqing and Quanzhou MHOs. We also observed different microbial functions in MUOs compared to MHOs, including an increased abundance of genes associated with glycan biosynthesis and metabolism. In addition, the microbial gene markers identified from the Chongqing cohort bear a moderate accuracy [AUC (area under the operating characteristic curve)=0.69] for classifying MHOs distinct from MUOs in the Quanzhou cohort. These findings indicate that gut microbiome is significantly distinct between MHOs and MUOs, implicating the potential of the gut microbiome in stratification and refined management of obesity.
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http://dx.doi.org/10.1099/mgen.0.000639DOI Listing
August 2021

fruit extract exerts anticonvulsant effects through suppression of oxidative stress and neuroinflammation in a pentylenetetrazol kindling model of epilepsy in mice.

Saudi J Biol Sci 2021 Aug 9;28(8):4247-4254. Epub 2021 Jun 9.

Department of Neurology, Xi'an Yanliang District People's Hospital, Xi'an 710089, China.

Background: Chronic epilepsy is a multifaceted common brain disorder with manifold underlying factors. Epilepsy affects around 70 million peoples worldwide. is a perennial herbaceous plant that is extensively cultivated in many provinces of China reported to exert immense biological activities.

Objective: This research work was aimed to reveal the therapeutic actions of ethanolic extract of fruits (EE-ATF) against the pentylenetetrazol (PTZ)-provoked convulsive seizures in the mice.

Methodology: The convulsive seizures were provoked to the animals via administering 70 mg/kg of PTZ through intraperitoneally to trigger the convulsive seizures then treated with the EE-ATF at 50, 75, and 100 mg/kg orally 30 min prior to PTZ challenge. After the 30 min of PTZ challenge, animals closely monitored for signs of convulsion, generalized clonic and tonic convulsion durations, and mortality. A sub-convulsive dose 35 mg/kg of PTZ was used to provoke the kindling and seizure stages were examined using standard method. The levels of dopamine, GABA, glutamate, and Na + K + ATPase and Ca + ATPase activities in the brain tissues were studied using marker specific assay kits. The oxidative stress and antioxidant markers studied using standard methods. The mRNA expressions of COX-2, TNF-α, NF-κB, TLR-4, and IL-1β in the brain tissues were studied using RT-PCR analysis. The brain tissues were examined histologically.

Results: EE-ATF treatment remarkably decreased the onset and duration of convulsion and suppressed the seizure severity and mortality in the PTZ animals. EE-ATF treatment appreciably ameliorated the PTZ triggered modifications in the GABA, glutamate, dopamine levels and Ca + 2ATPase and Na + K + ATPase activities in the brain tissues. EE-ATF suppressed the mRNA expressions of NF-κB, IL-1β, TLR-4, TNF-α, and COX-2. The status of antioxidants were elevated by the EE-ATF. Histological findings also demonstrated the curative actions of EE-ATF.

Conclusion: Our findings evidenced that the EE-ATF substantially ameliorated the PTZ-provoked convulsive seizures in the mice.
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http://dx.doi.org/10.1016/j.sjbs.2021.06.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325006PMC
August 2021

Heat-induced down-regulation of photosystem II protects photosystem I in honeysuckle (Lonicera japonica).

J Plant Res 2021 Nov 5;134(6):1311-1321. Epub 2021 Aug 5.

Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, The College of Forestry, Beijing Forestry University, Beijing, 100083, China.

Honeysuckle (Lonicera japonica Thunb.) is a traditional medicinal plant in China which is often threatened by high temperature at midday during summer. Heat-induced effects on the photosynthetic apparatus in honeysuckle are associated with a depression of the photosystem II (PSII) photochemical efficiency. However, very limited information is available on regulation of photosynthetic electron flow in PSI photoprotection in heat-stressed honeysuckle. Simultaneous analyses of chlorophyll fluorescence and the change in absorbance of P700 showed that energy transformation and electron transfer activity in PSII decreased under heat stress, but the fraction of photo-oxidizable PSI (Pm) remained stable. With treatments at 38 and 42 °C, the photochemical electron transport in PSII was suppressed, whereas the cyclic electron flow (CEF) around PSI was induced. In addition, the levels of high energy state quenching (qE) and P700 oxidation increased significantly with increasing temperature. However, a decline of qE in antimycin A (AA)- or 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU)-treated leaves after heat treatment was observed, while P700 oxidation decreased only in the presence of AA. The results indicate that heat-induced inhibition of PSII and induction of CEF cooperatively protect PSI from ROS damages through moderate down-regulation of photosynthetic electron flow from PSII to PSI.
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http://dx.doi.org/10.1007/s10265-021-01336-xDOI Listing
November 2021

In-depth investigation of the effective substances of traditional Chinese medicine formula based on the novel concept of co-decoction reaction-using Zuojin decoction as a model sample.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Aug 24;1179:122869. Epub 2021 Jul 24.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address:

Zuojin decoction (ZJD) is a classic pair composed of Coptidis Rhizoma and Evodiae Fructus, which is suitable for treating gastrointestinal diseases and tumours, etc. In recent years, scientists have been widely focused on research into the treatment of liver cancer using ZJD; however, the effective substances have not yet been comprehensively elucidated. The difference between the co-decoction and the single decoction of ZJD is revealed in this paper based on the UPLC-QE-Orbitrap-MS, and the chemical components absorbed into the blood and liver of mice have been analyzed simultaneously. In addition, the combination of prototype components absorbed into the liver with liver cancer-related targets has been performed via molecular docking to explore the mechanism of ZJD in treating liver cancer. By comparing the co-decoction and single decoction of ZJD, 44 new components appeared during co-decoction and 76 known chemical compounds have been identified at the same time. It has been confirmed that 35 known components and 11 new components were absorbed into the blood. Furthermore, 20 known components were discovered from the sample of liver tissue. Molecular docking results showed that 3-O-feruloylquinic acid has good conjugation with Bcl-2, Stat3, mTOR, and mmp9. Catechin has the lowest binding energy with CDK6 and β-catenin. The study provides data for the further confirmation of the material basis and mechanism of ZJD in treating liver cancer, and provides a new idea for the researches on the compatibility mechanism of prescriptions of traditional Chinese medicine.
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http://dx.doi.org/10.1016/j.jchromb.2021.122869DOI Listing
August 2021

The Expression Patterns and Roles of Lysyl Oxidases in Aortic Dissection.

Front Cardiovasc Med 2021 7;8:692856. Epub 2021 Jul 7.

Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Lysyl oxidases (LOXs), including LOX, LOXL1, LOXL2, LOXL3, and LOXL4, catalyze the formation of a cross-link between elastin (ELN) and collagen. Multiple LOX mutations have been shown to be associated with the occurrence of aortic dissection (AD) in humans, and LOX-knockout mice died during the perinatal period due to aortic aneurysm and rupture. However, the expression levels and roles of other LOX members in AD remain unknown. A total of 33 aorta samples of AD and 15 normal aorta were collected for LOXs mRNA and protein levels detection. We also analyzed the datasets of AD in GEO database through bioinformatics methods. LOXL2 and LOXL3 were knocked down in primary cultured human aortic smooth muscle cells (HASMCs) via lentivirus. Here, we show that the protein levels of LOXL2 and LOXL3 are upregulated, while LOXL4 is downregulated in AD subjects compared with non-AD subjects, but comparable protein levels of LOX and LOXL1 are detected. Knockdown of LOXL2 suppressed MMP2 expression, the phosphorylation of AKT (p-AKT) and S6 (p-S6), but increased the mono-, di-, tri-methylation of H3K4 (H3K4me1/2/3), H3K9me3, and p-P38 levels in HASMCs. These results indicate that LOXL2 is involved in regulation of the extracellular matrix (ECM) in HASMCs. In contrast, LOXL3 knockdown inhibited PCNA and cyclin D1, suppressing HASMC proliferation. Our results suggest that in addition to LOX, LOXL2 and LOXL3 are involved in the pathological process of AD by regulating ECM and the proliferation of HASMCs, respectively. Furthermore, we found that LOXL2 and LOXL4 was inhibited by metformin and losartan in HASMCs, which indicated that LOXL2 and LOXL4 are the potential targets that involved in the therapeutic effects of metformin and losartan on aortic or aneurysm expansion. Thus, differential regulation of LOXs might be a novel strategy to prevent or treat AD.
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http://dx.doi.org/10.3389/fcvm.2021.692856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292648PMC
July 2021

An autofluorescence-based isolation of Leydig cells for testosterone deficiency treatment.

Mol Cell Endocrinol 2021 09 3;535:111389. Epub 2021 Jul 3.

Department of Andrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. Electronic address:

Effective procedures for the purification of Leydig cells (LCs) can facilitate functional studies and transplantation therapies. However, current methods to purify LCs from testes are still far from satisfactory. Here, we found that testicular autofluorescence existed in the interstitium along with the gradual maturation of LCs from birth to adulthood. These autofluorescent cells were further isolated by fluorescence-activated cell sorting (FACS) and determined to be composed of LCs and macrophages. To further purify LCs, we combined two fluorescence channels of FACS and successfully separated LCs and macrophages. Of note, we confirmed that the obtained LCs not only possessed high purity, viability and quantity but also had intact steroidogenic activity and excellent responsiveness to luteinizing hormone. Moreover, subcutaneous transplantation of isolated LCs could alleviate the symptoms of testosterone deficiency in castrated mice. In summary, we established an effective autofluorescence-based method for isolating LCs. This method will aid in the future success of using LCs for basic and translational applications.
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http://dx.doi.org/10.1016/j.mce.2021.111389DOI Listing
September 2021

Tungsten-Modulated Molybdenum Selenide/Graphene Heterostructure as an Advanced Electrode for All-Solid-State Supercapacitors.

Nanomaterials (Basel) 2021 Jun 2;11(6). Epub 2021 Jun 2.

The State Key Discipline Laboratory of Wide Band Gap Semiconductor Technology, Xidian University, Xi'an 710071, China.

Transition metal dichalcogenides (TMDs) have attracted widespread attention due to their excellent electrochemical and catalytic properties. In this work, a tungsten (W)-modulated molybdenum selenide (MoSe)/graphene heterostructure was investigated for application in electrochemistry. MoSe/graphene heterojunctions with low-doped W compositions were synthesized by a one-step hydrothermal catalysis approach. Based on the conducted density functional theory (DFT) calculations, it was determined that inserting a small amount of W (≈5%) into the MoSe/graphene heterostructure resulted in the modification of its lattice structure. Additionally, an increase in the distance between layers (≈8%) and a decrease in the adsorption energy of the potassium ions (K) (≈-1.08 eV) were observed following W doping. Overall, the electrochemical performance of the MoSe/graphene hybrid was enhanced by the presence of W. An all-solid-state supercapacitor device prepared using electrodes based on the W-doped MoSe/graphene composite achieved excellent capacitance of 444.4 mF cm at 1 mV s. The results obtained herein revealed that the MoSe/graphene hybrid exhibiting low W composition could be valuable in the field of energy storage and isoelectronic doping of TMDs.
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http://dx.doi.org/10.3390/nano11061477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228879PMC
June 2021
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