Publications by authors named "Xin Fan"

368 Publications

Biocatalytic Nanomaterials: A New Pathway for Bacterial Disinfection.

Adv Mater 2021 Jul 3:e2100637. Epub 2021 Jul 3.

Department of Chemistry and Biochemistry, Freie Universität Berlin, Takustraße 3, 14195, Berlin, Germany.

Clinical treatment of pathogenic infection has emerged as a growing challenge in global public health. Such treatment is currently limited to antibiotics, but abuse of antibiotics have induced multidrug resistance and high fatality rates in anti-infection therapies. Thus, it is vital to develop alternative bactericidal agents to open novel disinfection pathways. Drawing inspiration from elements of the human immune system that show great potential for controlling pathogens or regulating cell apoptosis, the design of biocatalytic nanomaterials (BCNs) have provided unrivaled opportunities for future antibacterial therapies. More significantly, BCNs exhibit various superior properties to immune cells and natural enzymes, such as higher biocatalytic performance, extraordinary stability against harsh conditions, and scalable production. In this review, the most recent efforts toward developing BCN-based biomedical applications in combating bacterial infections are focused upon. BCNs' antibacterial mechanisms, the classification of BCNs, antibacterial activities that can be triggered or augmented by energy conversion, and the eradication of biofilms with BCNs are systematically introduced and discussed. The current challenges and prospects of BCNs for biocatalytic disinfection are also summarized. It is anticipated this review will provide new therapeutic insights into combating bacteria and biofilms and offer significant new inspiration for designing future biocatalytic nanomaterials.
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http://dx.doi.org/10.1002/adma.202100637DOI Listing
July 2021

Total Synthesis of (+)-3-Deoxyfortalpinoid F, (+)-Fortalpinoid A, and (+)-Cephinoid H.

Angew Chem Int Ed Engl 2021 Jun 23. Epub 2021 Jun 23.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education College of Chemistry & Materials Science, Northwest University, Xi'an, 710127, China.

3-Deoxyfortalpinoid F, fortalpinoid A, and cephinoid H are members of the Cephalotaxus diterpenoids class of natural products, which feature diverse chemical structures and valuable biological activities. We report herein the development of a diastereoselective Pauson-Khand reaction as an effective pathway to access the core tetracyclic skeleton, which is found widely in Cephalotaxus diterpenoids. Furthermore, we enabled the construction of the tropone moiety through a ring-closing metathesis/elimination protocol. Based on the developed strategy, asymmetric synthesis of the title compounds has been achieved for the first time.
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http://dx.doi.org/10.1002/anie.202108034DOI Listing
June 2021

A narrative review on the interaction between genes and the treatment of hypertension and breast cancer.

Ann Transl Med 2021 May;9(10):894

Department of Hypertension Center, Lanzhou University Second Hospital, Lanzhou, China.

Objective: The aim to discuss the close relationship between the common biological mechanisms of breast cancer and hypertension, inflammation and oxidative stress, breast cancer gene mutations breast cancer susceptibility gene (BRCA), G protein-coupled receptor kinase (GRK4), etc. and breast cancer treatment includes chemotherapy, Endocrine therapy, Targeted therapy and anti-angiogenesis drugs. In anti-angiogenesis drugs focusing on the mechanism of tyrosine kinase inhibitors (TKI) that may activate the rhoa/rock pathway to cause hypertension, as well as the relationship between breast cancer and antihypertensive drugs includes angiotensin-converting enzyme inhibitors (ACEIs), Calcium channel blockers (CCBs) and β-blockers (BBs)will be explored.

Background: Cardiovascular diseases (CVD) and tumors are the two major types of diseases with the highest mortality rates, while hypertension accounts for the largest proportion of CVDs. A large number of the same or similar risk factors are shared between hypertension and tumors, and they influence each other. Many patients, particularly elderly patients, often present with the coexistence of the two diseases. As medical advances have enabled clinicians to cure tumors, many patients with cancer live longer, leading to a gradual increase in the incidence of CVDs, including hypertension. With the second highest incidence among tumors, breast cancer has gradually attracted widespread attention and has been the topic of numerous studies. Studies have confirmed that CVD is one of the causes of death in elderly patients with breast cancer.

Methods: Publications from 1985 to 2020 were retrieved from the Web Of Science, Cochrane Library, PubMed, EMBASE and MEDLINE database. We used a mix of MeSH and keywords.

Conclusions: Hypertension and cancer may share a common mechanism. The screening and risk assessment of breast cancer in patients with hypertension must be strengthened. Breast cancer cardiology is the interdisciplinary study of oncology and cardiology, and in-depth research in this field may result in long-term improvements in the survival and prognosis of patients with both clinical hypertension and breast cancer.
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http://dx.doi.org/10.21037/atm-21-2133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184430PMC
May 2021

Cytokines: Application in recurrence appraisal for differentiated thyroid carcinoma and their relation with radioiodine ablation.

J Cell Biochem 2021 Jun 18. Epub 2021 Jun 18.

Shanghai Clinical College, Anhui Medical University, Shanghai, China.

The limitations in discriminating preablation disease-active status of differentiated thyroid carcinoma (DTC) still represent a major challenge to radioiodine dose management. Cytokines, the small protein signaling molecules that constitute the thyroid tumor microenvironment, play significant roles in the facilitation of intercellular communication and the control of tumorigenesis. Also, more attention should be paid to the molecular events within the innate and adaptive immune systems that occur after the organism being exposed to ionizing radiation. Therefore, we implemented a study of 260 patients with DTC in thyroid hormone withdrawal status who were treated with total thyroidectomy to explore the relationship between cytokines and recurrence/active disease status. Besides, we made a cross-sectional study to analyze pre- and post-ablation serum concentration of cytokines of 86 patients with DTC. There was a relationship between clinicohistopathological characteristics of patients with DTC and the presence of cytokines. It is noteworthy that patients with recurrence/active disease were at a higher serum interleukin-2 receptor (IL-2R) level than the disease-free patients (213.59 ± 75.43 pg/ml vs. 186.80 ± 77.40 pg/ml, P = 0.005). Positive correlation was observed between serum IL-2R and thyroglobulin (Tg) (P = 0.003). We also found significant changes in the cytokine profile after radioiodine ablation, including the decrease of tumor necrosis factor-α and IL-8 (P < 0.001, P < 0.001, respectively), and increase of IL-2R (P < 0.001). Thus, we suggest that serum IL-2R may assist in evaluating the disease status during the post-thyroidectomy follow-up and radioiodine therapy has an immunoregulatory effect on serum cytokines.
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http://dx.doi.org/10.1002/jcb.30016DOI Listing
June 2021

A Novel Role of AR in the Maintenance of Intestinal Barrier Function of Enteric Glia from Hypoxia-Induced Injury by Combining with mGluR5.

Front Pharmacol 2021 10;12:633403. Epub 2021 May 10.

Department of General Surgery, Xinqiao Hospital, Army Medical University, Chongqing, China.

During acute intestinal ischemia reperfusion (IR) injury, the intestinal epithelial barrier (IEB) function is often disrupted. Enteric glial cells (EGCs) play an important role in maintaining the integrity of IEB functions. However, how EGCs regulate IEB function under IR stimulation is unknown. The present study reveals that the adenosine A receptor (AR) is important for mediating the barrier-modulating roles of EGCs. AR knockout (KO) experiments revealed more serious intestinal injury in AR KO mice than in WT mice after IR stimulation. Moreover, AR expression was significantly increased in WT mice when challenged by IR. To further investigate the role of AR in IEB, we established an EGC-Caco-2 co-culture system. Hypoxia stimulation was used to mimic the process of IR. Treating EGCs with the CGS21680 AR agonist attenuated hypoxia-induced intestinal epithelium damage through up-regulating ZO-1 and occludin expression in cocultured Caco-2 monolayers. Furthermore, we showed that AR and metabotropic glutamate receptor 5 (mGluR5) combine to activate the PKCα-dependent pathway in conditions of hypoxia. This study shows, for the first time, that hypoxia induces AR-mGluR5 interaction in EGCs to protect IEB function via the PKCα pathway.
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http://dx.doi.org/10.3389/fphar.2021.633403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173626PMC
May 2021

Two New Brackish-Water Species of (Platyhelminthes: Macrostomorpha) from China and Their Phylogenetic Positions.

Zoolog Sci 2021 Jun;38(3):273-286

Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, PR China,

In this paper, two new brackish-water species of the macrostomid turbellarian genus , sp. nov. and sp. nov., collected from coastal water at Shenzhen, Guangdong Province, China, are described based on morphological, histological, and molecular phylogenetic analyses. sp. nov. differs from similar species within the genus in the length of the stylet (152 15.0 µm), diameter of stylet opening (20 4.0 µm proximally; 7 ± 0.5 µm distally), two bends of the stylet, and the non-spiral end of the stylet. sp. nov. differs from its congeners in the length of the stylet (81 7.4 µm), the stylet bending position and angle (50% and 60°), diameter of stylet proximal opening (15 ± 3.0 µm), sperm with bristles and brush, and the smooth-walled ovaries. Phylogenetic analyses inferred from nuclear 18S and 28S rRNA genes support the establishments of these two new species. In addition, reciprocal mating behavior of sp. nov. was observed and documented.
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http://dx.doi.org/10.2108/zs200121DOI Listing
June 2021

Prevalence and impact of fibrinolytic dysregulation in patients with acute coronary syndromes.

Thromb J 2021 May 22;19(1):33. Epub 2021 May 22.

Department of Cardiology, Dongguan People's Hospital, Southern Medical University, Dongguan, 523059, Guangdong, China.

Objective: Dual antiplatelet therapy can reduce coronary thrombosis and improve the prognosis in patients with acute coronary syndrome (ACS). However, there was limited prognostic information about fibrinolytic dysregulation in patients with ACS. This study is aimed to evaluated the prevalence and impact of fibrinolytic dysregulation in patients with acute coronary syndrome (ACS).

Methods: We retrospectively analyzed coagulation and fibrinolysis related indexes of ACS in hospitalized adults with rapid thrombelastography between May 2016 and December 2018. All of the follow-up visits were ended by December 2019. The primary outcome was the occurrence of major adverse cardiovascular events (MACEs), which included unstable angina pectoris, non-fatal myocardial infarction, non-fatal cerebral infarction, heart failure and all-cause death.

Results: Three hundred thirty-eight patients were finally included with an average age of 62.5 ± 12.8 years old, 273 (80.5%) were males, 137(40.5%) patients were with ST-elevation myocardial infraction. Fibrinolysis shutdown (LY30<0.8%) and hyperfibrinolysis (LY30 >3.0%) were observed among 163 (48.2%) and 76(22.5%) patients, respectively. During a total of 603.2 person·years of follow-up period, 77 MACEs occurred (22.8%). Multivariate Cox regression analysis indicated that LY30 [HR: 1.101, 95% CI: 1.010-1.200, P = 0.028] was independently correlated with the occurrence of MACEs. The hazard ratios pertaining to MACEs in patients with fibrinolysis shutdown and hyperfibrinolysis compared with those in the physiologic range (LY30: 0.8-3.0%) were 1.196 [HR: 1.196, 95% CI: 0.679-2.109,P = 0.535] and 2.275 [HR: 2.275, 95% CI: 1.241-4.172, P = 0.003], respectively.

Conclusions: Fibrinolytic dysregulation is very common in selected patients with ACS, and hyperfibrinolysis (LY30 > 3%) is associated with poor outcomes in patients with ACS.
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http://dx.doi.org/10.1186/s12959-021-00288-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141118PMC
May 2021

Exome sequencing reveals genetic architecture in patients with isolated or syndromic short stature.

J Genet Genomics 2021 Mar 22. Epub 2021 Mar 22.

Department of Endocrinology, Genetics and Metabolism, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China. Electronic address:

Short stature is among the most common endocrinological disease phenotypes of childhood and may occur as an isolated finding or in conjunction with other clinical manifestations. Although the diagnostic utility of clinical genetic testing in short stature has been implicated, the genetic architecture and the utility of genomic studies such as exome sequencing (ES) in a sizable cohort of patients with short stature have not been investigated systematically. In this study, we recruited 561 individuals with short stature from two centers in China during a 4-year period. We performed ES for all patients and available parents. All patients were retrospectively divided into two groups: an isolated short stature group (group I, n = 257) and an apparently syndromic short stature group (group II, n = 304). Causal variants were identified in 135 of 561 (24.1%) patients. In group I, 29 of 257 (11.3%) of the patients were solved by variants in 24 genes. In group II, 106 of 304 (34.9%) patients were solved by variants in 57 genes. Genes involved in fundamental cellular process played an important role in the genetic architecture of syndromic short stature. Distinct genetic architectures and pathophysiological processes underlie isolated and syndromic short stature.
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http://dx.doi.org/10.1016/j.jgg.2021.02.008DOI Listing
March 2021

Heterozygous Recurrent Mutations Inducing Dysfunction of Gene in Patients With Short Stature.

Front Cell Dev Biol 2021 14;9:661747. Epub 2021 Apr 14.

Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Purpose: ROR2, a member of the ROR family, is essential for skeletal development as a receptor of Wnt5a. The present study aims to investigate the mutational spectrum of in children with short stature and to identify the underlying molecular mechanisms.

Methods: We retrospectively analyzed clinical phenotype and whole-exome sequencing (WES) data of 426 patients with short stature through mutation screening of . We subsequently examined the changes in protein expression and subcellular location in caused by the mutations. The mRNA expression of downstream signaling molecules of the Wnt5a-ROR2 pathway was also examined.

Results: We identified 12 mutations in in 21 patients, including 10 missense, one nonsense, and one frameshift. Among all missense variants, four recurrent missense variants [c.1675G > A(p.Gly559Ser), c.2212C > T(p.Arg738Cys), c.1930G > A(p.Asp644Asn), c.2117G > A(p.Arg706Gln)] were analyzed by experiments . The c.1675G > A mutation significantly altered the expression and the cellular localization of the ROR2 protein. The c.1675G > A mutation also caused a significantly decreased expression of c-Jun. In contrast, other missense variants did not confer any disruptive effect on the biological functions of ROR2.

Conclusion: We expanded the mutational spectrum of in patients with short stature. Functional experiments potentially revealed a novel molecular mechanism that the c.1675G > A mutation in might affect the expression of downstream Wnt5a-ROR2 pathway gene by disturbing the subcellular localization and expression of the protein.
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http://dx.doi.org/10.3389/fcell.2021.661747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080376PMC
April 2021

Synergistic recovery and enhancement of gelling properties of oxidatively damaged myofibrillar protein by -lysine and transglutaminase.

Food Chem 2021 Oct 20;358:129860. Epub 2021 Apr 20.

Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, United States. Electronic address:

The influence of combined Lysine (Lys) and transglutaminase (TG) on the conformation and gelling properties of oxidatively damaged myofibrillar protein (MP) was investigated. The addition of Lys (5 mM) significantly increased the α-helix content (by 47.8%) and decreased the particle size of oxidatively damaged MP, and improved the cooking yield (by 16.8%) and the breaking strength of MP gels (by 65.5%). The treatment with TG (E:S = 1:500) led to a slightly reduced α-helix content but improved breaking strength (by 41.8%) and cooking loss (by 13.3%) of the gels. Their combination (Lys + TG) showed the greatest and synergistic overall improvement, with the set gel displaying a fine, smooth and compact network structure. Notably, the gelling ability of oxidatively damaged MP upon Lys + TG treatment was significantly stronger than that of non-oxidized MP far exceeding its recovery. Therefore, significantly enhanced gelling properties of oxidatively damaged MP can be attained through the combination Lys and TG.
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http://dx.doi.org/10.1016/j.foodchem.2021.129860DOI Listing
October 2021

A multi-center longitudinal study on responsive breastfeeding in China from the perspective of health equity: research protocol.

Int J Equity Health 2021 May 1;20(1):111. Epub 2021 May 1.

Department of Child, Adolescent Health and Maternal Care, School of Public Health, Capital Medical University, No.10 You'anmenwai Xitoutiao, Fengtai District, Beijing, 100069, China.

Background: Socio-economic inequities can strongly influence suboptimal infant feeding outcomes. Factors such as lack of knowledge about breastfeeding, low family income, low educational attainment, social and economic status, cultural norms and ethnicity may negatively affect success with offering breastfeeding following a responsive feeding approach (ie. responsive breastfeeding). Such inequities can indeed shorten breastfeeding duration, and negatively affect behavioral and cognitive infant outcomes. In China, there is a dearth of studies focusing on breastfeeding from the responsive and health equity perspective.

Objective: The aim of this article is to present a protocol of an ongoing longitudinal cohort study investigating factors associated with responsive breastfeeding behaviors, and the child's behavioral and cognitive development from birth to12 months post-partum in five centers in China. The study seeks to identify breastfeeding barriers and facilitators from a health equity perspective.

Methods: We are enrolling 700 women and their singleton full term infants in Chongqing, Huizhou and Guangzhou urban and rural areas. The study questionnaires will be administrated within 72 h, 30 days, 3, 6, 9, and 12 months post-partum during the baby's vaccination visits. We will investigate the difference between urban and rural areas sociodemographic characteristics, breastfeeding knowledge, attitudes and practice, postnatal depression, maternal emotion regulation and parenting stress, and anthropometric and cognitive development indicators of the infants at each time-point.

Conclusion: Our article illustrates how a cohort study can be designed to understand the barriers and facilitators of responsive breastfeeding taking equity principles into account to help promote infants' growth and development in China.
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http://dx.doi.org/10.1186/s12939-021-01430-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087879PMC
May 2021

Learning Deep Context-Sensitive Decomposition for Low-Light Image Enhancement.

IEEE Trans Neural Netw Learn Syst 2021 Apr 30;PP. Epub 2021 Apr 30.

Enhancing the quality of low-light (LOL) images plays a very important role in many image processing and multimedia applications. In recent years, a variety of deep learning techniques have been developed to address this challenging task. A typical framework is to simultaneously estimate the illumination and reflectance, but they disregard the scene-level contextual information encapsulated in feature spaces, causing many unfavorable outcomes, e.g., details loss, color unsaturation, and artifacts. To address these issues, we develop a new context-sensitive decomposition network (CSDNet) architecture to exploit the scene-level contextual dependencies on spatial scales. More concretely, we build a two-stream estimation mechanism including reflectance and illumination estimation network. We design a novel context-sensitive decomposition connection to bridge the two-stream mechanism by incorporating the physical principle. The spatially varying illumination guidance is further constructed for achieving the edge-aware smoothness property of the illumination component. According to different training patterns, we construct CSDNet (paired supervision) and context-sensitive decomposition generative adversarial network (CSDGAN) (unpaired supervision) to fully evaluate our designed architecture. We test our method on seven testing benchmarks [including massachusetts institute of technology (MIT)-Adobe FiveK, LOL, ExDark, and naturalness preserved enhancement (NPE)] to conduct plenty of analytical and evaluated experiments. Thanks to our designed context-sensitive decomposition connection, we successfully realized excellent enhanced results (with sufficient details, vivid colors, and few noises), which fully indicates our superiority against existing state-of-the-art approaches. Finally, considering the practical needs for high efficiency, we develop a lightweight CSDNet (named LiteCSDNet) by reducing the number of channels. Furthermore, by sharing an encoder for these two components, we obtain a more lightweight version (SLiteCSDNet for short). SLiteCSDNet just contains 0.0301M parameters but achieves the almost same performance as CSDNet. Code is available at https://github.com/KarelZhang/CSDNet-CSDGAN.
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http://dx.doi.org/10.1109/TNNLS.2021.3071245DOI Listing
April 2021

Participation of the Anterior Cingulate Cortex in Sodium Salicylate-induced Tinnitus.

Otol Neurotol 2021 Apr 14. Epub 2021 Apr 14.

Department of Otolaryngology Head and Neck Surgery, Peking University Third Hospital Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, China.

Hypothesis: The anterior cingulate cortex (ACC) participates in sodium salicylate (SS)-induced tinnitus through alteration of the disordered neural activity and modulates the neuronal changes in the auditory cortex (AC).

Background: Although the mechanism underlying tinnitus remains unclear, the crucial roles of the auditory center and limbic system in this process have been elucidated. Recent reports suggest that dysfunction of the ACC, an important component of the limbic system that regulates and controls the conduction of multiple sensations, is involved in tinnitus. Although altered functional connectivity between the ACC and the auditory system has been observed in humans with tinnitus, the underlying neuronal mechanism remains unexplored.

Methods: SS (350 mg/kg, 10%, i.p.) was used to yield tinnitus model in rats, followed by comparison of the alteration in the spontaneous firing rate (SFR), local field potential (LFP), and extracellular glutamic acid in the ACC. The responses of neurons in the AC to electrical stimulation from the ACC were also observed.

Results: We determined significant increases in the neuronal SFR and extracellular glutamate level in the ACC after SS injection (p < 0.05). These effects were accompanied by decreased alpha band activity and increased beta and gamma band activity (p < 0.05). In the majority of AC neurons, the SFR decreased in response to ACC stimulation (p < 0.05).

Conclusions: Our results demonstrated that disordered neural activity in the ACC contributes to SS-induced tinnitus and that ACC activation can modulate AC activity.
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http://dx.doi.org/10.1097/MAO.0000000000003183DOI Listing
April 2021

Unsupervised Monocular Depth Estimation via Recursive Stereo Distillation.

IEEE Trans Image Process 2021 27;30:4492-4504. Epub 2021 Apr 27.

Existing unsupervised monocular depth estimation methods resort to stereo image pairs instead of ground-truth depth maps as supervision to predict scene depth. Constrained by the type of monocular input in testing phase, they fail to fully exploit the stereo information through the network during training, leading to the unsatisfactory performance of depth estimation. Therefore, we propose a novel architecture which consists of a monocular network (Mono-Net) that infers depth maps from monocular inputs, and a stereo network (Stereo-Net) that further excavates the stereo information by taking stereo pairs as input. During training, the sophisticated Stereo-Net guides the learning of Mono-Net and devotes to enhance the performance of Mono-Net without changing its network structure and increasing its computational burden. Thus, monocular depth estimation with superior performance and fast runtime can be achieved in testing phase by only using the lightweight Mono-Net. For the proposed framework, our core idea lies in: 1) how to design the Stereo-Net so that it can accurately estimate depth maps by fully exploiting the stereo information; 2) how to use the sophisticated Stereo-Net to improve the performance of Mono-Net. To this end, we propose a recursive estimation and refinement strategy for Stereo-Net to boost its performance of depth estimation. Meanwhile, a multi-space knowledge distillation scheme is designed to help Mono-Net amalgamate the knowledge and master the expertise from Stereo-Net in a multi-scale fashion. Experiments demonstrate that our method achieves the superior performance of monocular depth estimation in comparison with other state-of-the-art methods.
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http://dx.doi.org/10.1109/TIP.2021.3072215DOI Listing
April 2021

A Meta-Analysis of the Effects of Tai Chi on Glucose and Lipid Metabolism in Middle-Aged and Elderly Diabetic Patients: Evidence from Randomized Controlled Trials.

Evid Based Complement Alternat Med 2021 22;2021:6699935. Epub 2021 Mar 22.

School of Physical Education, Hubei Minzu University, Enshi 445000, China.

This research review aimed to evaluate the effect of practicing Tai Chi on glucose and lipid metabolism in middle-aged and elderly diabetic patients. Furthermore, it aimed to provide a theoretical basis for the practice of Tai Chi as a way to improve glucose and lipid metabolism in middle-aged and elderly diabetic patients. Therefore, we searched for randomized controlled trials on the practice of Tai Chi in middle-aged and elderly diabetic patients in Chinese- and English-language electronic databases, such as Web of Science, PubMed, the Cochrane Library, EMBASE, Google Scholar, CNKI, Wanfang Database, and Weipu. We collected articles published no later than August 1, 2020. The methodological quality of the included studies was evaluated according to the standards of the Cochrane Collaboration System Evaluation Manual (version 5.1.0). Finally, 14 articles were included, showing an average Physiotherapy Evidence Database scale score of 6.57. The articles were meta-analyzed using Stata 14.0 software, showing that practicing Tai Chi improved middle-aged and elderly diabetic patients' fasting blood glucose (WMD = -0.60, 95% CI [-1.08, -0.12], =0.015), glycosylated hemoglobin (WMD = -0.87, 95% CI [-1.60, -0.14], =0.019), total cholesterol (WMD = -0.48, 95% CI [-0.83, -0.14], =0.006), triglycerides (WMD = -0.21, 95% CI [-0.37, -0.04], =0.014), and low-density lipoprotein cholesterol level significantly (WMD = -0.32, 95% CI [-0.63,-0.00], =0.050). Conversely, patients' high-density lipoprotein cholesterol levels (WMD = 0.09, 95% CI [-0.01, 0.17], =0.136) showed no obvious improvement. In conclusion, practicing Tai Chi in sessions lasting longer than 50 minutes (at least three times per week, for at least 12 weeks) can effectively improve glucose and lipid metabolism in middle-aged and elderly diabetic patients. However, several other factors affect glucose and lipid metabolism; therefore, further high-quality research is needed. Protocol registration number: INPLASY2020120107.
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http://dx.doi.org/10.1155/2021/6699935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007338PMC
March 2021

Rotavirus-related systemic diseases: clinical manifestation, evidence and pathogenesis.

Crit Rev Microbiol 2021 Apr 6:1-16. Epub 2021 Apr 6.

Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.

Rotaviruses, double-stranded, non-enveloped RNA viruses, are a global health concern, associated with acute gastroenteritis and secretory-driven watery diarrhoea, especially in infants and young children. Conventionally, rotavirus is primarily viewed as a pathogen for intestinal enterocytes. This notion is challenged, however, by data from patients and animal models documenting extra-intestinal clinical manifestations and viral replication following rotavirus infection. In addition to acute gastroenteritis, rotavirus infection has been linked to various neurological disorders, hepatitis and cholestasis, type 1 diabetes, respiratory illness, myocarditis, renal failure and thrombocytopenia. Concomitantly, molecular studies have provided insight into potential mechanisms by which rotavirus can enter and replicate in non-enterocyte cell types and evade host immune responses. Nevertheless, it is fair to say that the extra-intestinal aspect of the rotavirus infectious process is largely being overlooked by biomedical professionals, and there are gaps in the understanding of mechanisms of pathogenesis. Thus with the aim of increasing public and professional awareness we here provide a description of our current understanding of rotavirus-related extra-intestinal clinical manifestations and associated molecular pathogenesis. Further understanding of the processes involved should prove exceedingly useful for future diagnosis, treatment and prevention of rotavirus-associated disease.
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http://dx.doi.org/10.1080/1040841X.2021.1907738DOI Listing
April 2021

Role of the caudate-putamen nucleus in sensory gating in induced tinnitus in rats.

Neural Regen Res 2021 Nov;16(11):2250-2256

Department of Otolaryngology Head and Neck Surgery, Peking University Third Hospital, Beijing, China.

Tinnitus can be described as the conscious perception of sound without external stimulation, and it is often accompanied by anxiety, depression, and insomnia. Current clinical treatments for tinnitus are ineffective. Although recent studies have indicated that the caudate-putamen nucleus may be a sensory gating area involved in noise elimination in tinnitus, the underlying mechanisms of this disorder are yet to be determined. To investigate the potential role of the caudate-putamen nucleus in experimentally induced tinnitus, we created a rat model of tinnitus induced by intraperitoneal administration of 350 mg/kg sodium salicylate. Our results revealed that the mean spontaneous firing rate of the caudate-putamen nucleus was increased by sodium salicylate treatment, while dopamine levels were decreased. In addition, electrical stimulation of the caudate-putamen nucleus markedly reduced the spontaneous firing rate of neurons in the primary auditory cortex. These findings suggest that the caudate-putamen nucleus plays a sensory gating role in sodium salicylate-induced tinnitus. This study was approved by the Institutional Animal Care and Use Committee of Peking University Health Science Center (approval No. A2010031) on December 6, 2017.
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http://dx.doi.org/10.4103/1673-5374.310692DOI Listing
November 2021

Effect of chromodomain helicase/ATPase DNA binding protein 1-like gene on the invasion and metastasis of tongue squamous cell carcinoma CAL27 cells.

Hua Xi Kou Qiang Yi Xue Za Zhi 2021 Feb;39(1):81-87

Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Weifang Medical University, Weifang 261000, China.

Objectives: A study was conducted to investigate the molecular mechanism of chromodomain helicase/ATPase DNA binding protein 1-like gene (CHD1L) influencing the invasion and metastasis of tongue squamous cell carcinoma and to provide a new target for clinical inhibition of invasion and metastasis of tongue squamous cell carcinoma.

Methods: Ualcan website was used to analyze the expression of CHD1L in normal epithelial tissue and primary head and neck squamous cell carcinoma and to analyze the effect of lymph node metastasis on the expression of CHD1L in tissues with head and neck squamous cell carcinoma. The relationship between CHD1L expression and the survival rate of patients with head and neck squamous cell carcinoma was tested by the GEPIA website. Western blot was used to quantify the levels of CHD1L protein in human tongue squamous cell carcinoma CAL27 and immortalized human skin keratinocyte cell HaCaT. After knocking down CAL27 in human tongue squamous cell carcinoma cells with an RNA interference plasmid, the cells were designated as SiCHD1L/CAL27 and Scr/CAL27. Western blot was utilized to detect the expression of CHD1L in each group of cells. The change in CAL27 cell proliferation ability was tested by EdU proliferation test after CHD1L knockdown. The change of cell migration ability of each group cells was tested through the wound healing assay. Western blot was used to detect epithelial-mesenchymal transition (EMT) marker E-cadherin and Vimentin protein expression levels.

Results: Ualcan database showed that the expression of CHD1L in primary head and neck squamous cell carcinoma tissues was higher than in normal epithelial tissues and in head and neck squamous cell carcinoma tissues with lymph node metastasis. GEPIA website analysis showed that the overall survival rate of patients with head and neck squamous cell carcinoma with high expression of CHD1L was significantly lower than that of patients with low expression. Western blot results showed that CHD1L expression in human tongue squamous carcinoma cells CAL27 was higher than that of human normal skin cells HaCaT. CHD1L expression in SiCHD1L/CAL27 cells was much lower than that in Scr/CAL27 cells. Results of EdU proliferation experiments showed the significant reduction in the cell proliferation ability of the SiCHD1L/CAL27 cells. Results of the wound healing experiments showed the reduction in the migration capacity of the SiCHD1L/CAL27 cells. The expression of E-cadherin increased, whereas that of Vimentin decreased, in SiCHD1L/CAL27 cells.

Conclusions: CHD1L promoted the EMT, proliferation, migration, and invasion ability of tongue squamous cell carcinoma cells.
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http://dx.doi.org/10.7518/hxkq.2021.01.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905417PMC
February 2021

Evaluation of Autof MS 1000 and Vitek MS MALDI-TOF MS System in Identification of Closely-Related Yeasts Causing Invasive Fungal Diseases.

Front Cell Infect Microbiol 2021 18;11:628828. Epub 2021 Feb 18.

Department of Laboratory Medicine, and Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been accepted as a rapid, accurate, and less labor-intensive method in the identification of microorganisms in clinical laboratories. However, there is limited data on systematic evaluation of its effectiveness in the identification of phylogenetically closely-related yeast species. In this study, we evaluated two commercially available MALDI-TOF systems, Autof MS 1000 and Vitek MS, for the identification of yeasts within closely-related species complexes. A total of 1,228 yeast isolates, representing 14 different species of five species complexes, including 479 of complex, 323 of complex, 95 of complex, 16 of complex (including two ), and 315 of complex, collected under the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) program, were studied. Autof MS 1000 and Vitek MS systems correctly identified 99.2% and 89.2% of the isolates, with major error rate of 0.4% versus 1.6%, and minor error rate of 0.1% versus 3.5%, respectively. The proportion of isolates accurately identified by Autof MS 1000 and Vitek MS per each yeast complex, respectively, was as follows; complex, 99.4% vs 96.3%; complex, 99.0% vs 79.1%; complex, 98.9% vs 94.7%; complex, 100% vs 93.8%; and , 99.4% vs 95.2%. Overall, Autof MS 1000 exhibited good capacity in yeast identification while Vitek MS had lower identification accuracy, especially in the identification of less common species within phylogenetically closely-related species complexes.
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http://dx.doi.org/10.3389/fcimb.2021.628828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930211PMC
July 2021

Three-dimensional printing combined with open reduction and internal fixation versus open reduction and internal fixation in the treatment of acetabular fractures: A systematic review and meta-analysis.

Chin J Traumatol 2021 May 27;24(3):159-168. Epub 2021 Feb 27.

Zhejiang Chinese Medical University, Hangzhou, 310053, China; Department of Orthopaedic, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310005, China. Electronic address:

Purpose: This meta-analysis compared the clinical outcome of three-dimensional (3D) printing combined with open reduction and internal fixation (ORIF) to traditional ORIF in the treatment of acetabular fractures.

Methods: We searched the Cochrane Library, PubMed, Embase, VIP database, CNKI, and Wanfang database with keywords "acetabular fracture", "3D printing", "three-dimensional printing", "open reduction and internal fixation", "Acetabulum", "Acetabula" from January 2000 to March 2020. Two reviewers independently selected articles, extracted data, assessed the quality evidence and risk bias of included trials using the Cochrane Collaboration' s tools and/or Newcastle-Ottawa scale. When the two analysts had different opinions, they would ask the third analyst for opinion. Randomized controlled trials or retrospective comparative studies of 3D printing combined with ORIF (3D printing group) versus traditional ORIF (conventional group) in the treatment of acetabular fractures were selected. The data of operation time, intraoperative blood loss, intraoperative fluoroscopy times, incidence of complications, excellent and good rate of Matta score for reduction, and excellent and good rate of hip function score were extracted. Stata14.0 statistical software was used for data analysis.

Results: Altogether 9 articles were selected, including 5 randomized controlled trials and 4 retrospective studies. A total of 467 patients were analyzed, 250 in the conventional group, and 217 in the 3D printing group. The operation time in the 3D printing group was less than that in the conventional group and the difference was statistically significant (standardized mean difference (SMD) = -1.19, 95% CI: -1.55 to -0.82, p < 0.05). The intraoperative bleeding volume of the 3D printing group was significantly lower than that of the conventional group (SMD = -1.08, 95% CI: -1.65 to -0.51, p < 0.05). The fluoroscopy times were less in the 3D printing group than in the conventional group and the difference was statistically significant (SMD = -1.64, 95% CI: -2.35 to -0.93, p < 0.05). The total incidence of complications in the 3D printing group was significantly lower than that in the conventional group (OR = 0.43, 95% CI: 0.24-0.79, p < 0.05). There was no significant difference in the excellent and good rate of Matta score for reduction between the two groups (OR = 0.60, 95% CI: 0.34-1.06, p > 0.05). There was no significant difference in the excellent and good rate of hip function score at the end of postoperative follow-up between the two groups (OR = 0.84, 95% CI: 0.46-1.56, p > 0.05), but the follow-up time varies from 6 months to 40 months.

Conclusion: Compared with traditional ORIF, 3D printing combined with ORIF has certain advantages in terms that 3D printing not only helps surgeons to understand acetabular fractures more intuitively, but also effectively reduces operation time, intraoperative blood loss, intraoperative fluoroscopy times, and postoperative complications. However, there were no significant differences in the excellent and good rate of Matta score for reduction and the excellent and good rate of hip function score at the end of follow-up.
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http://dx.doi.org/10.1016/j.cjtee.2021.02.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173577PMC
May 2021

BAF57/SMARCE1 Interacting with Splicing Factor SRSF1 Regulates Mechanical Stress-Induced Alternative Splicing of Cyclin D1.

Genes (Basel) 2021 02 21;12(2). Epub 2021 Feb 21.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 401120, China.

Cyclin D1 regulates cyclin-dependent protein kinase activity of the cell cycle, and alternative splicing generates a isoform, acting as a mediator of aberrant cellular proliferation. As alternative splicing processes are sensitive to mechanical stimuli, whether the alternative splicing of is regulated by mechanical stress and what kinds of factors may act as the regulator of mechano-induced alternative splicing remain unknown. The alternative splicing of was examined using reverse transcription polymerase chain reaction (RT-PCR) in osteoblast cell lines and keratinocyte cells loaded by a cyclic stretch. The expression of splicing factors and switching defective/sucrose non-fermenting (SWI/SNF) complex subunits were detected in stretched cells using real-time quantitative PCR (RT-qPCR). The protein interaction was tested by co-immunoprecipitation assay (Co-IP). expression decreased with its splice variant upregulated in stretched cells. Serine/arginine-rich splicing factor 1 (SRSF1) and SWI/SNF complex subunit Brahma-related gene-1-associated factor 57 (BAF57), also named SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 (SMARCE1), could respond to mechanical stimuli. Overexpression and knockdown experiments indicated the BAF57/SMARCE1 is probably a critical factor regulating the alternative splicing of cyclin D1. Co-IP showed an interaction between BAF57/SMARCE1 and SRSF1, implying a possible underlying mechanism of the regulator role of BAF57/SMARCE1 in the splicing process of cyclin D1. The splicing factor SRSF1 and BAF57/SMARCE1 are possibly responsible for the mechanical stress-induced alternative splicing of cyclin D1.
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http://dx.doi.org/10.3390/genes12020306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927079PMC
February 2021

Expression, Rapid Purification and Functional Analysis of DnaK from Rhodococcus ruber.

Protein Pept Lett 2021 Mar 1. Epub 2021 Mar 1.

College of Life Science, Jiangxi Normal University, Nanchang-330022. China.

Background: Heat shock proteins (HSPs) represent a group of important proteins which are produced by all kinds of organisms especially under stressful conditions. DnaK, an Hsp70 homolog in prokaryotes, has indispensable roles when microbes was confronted with stress conditions. However, few data on DnaK from Rhodococcus sp. were available in the literature. In a previous study, we reported that toluene and phenol stress gave rise to a 29.87-fold and 3.93-fold increase for the expression of DnaK from R. ruber SD3, respectively. Thus, we deduced DnaK was in correlation with the organic solvent tolerance of R. ruber SD3.

Objective: To elucidate the role of DnaK in the organic solvent tolerance of R. ruber SD3, expression, purification and functional analysis of Dnak from R. ruber SD3 were performed in the present paper.

Methods: In this article, DnaK from R. ruber SD3 was heterologously expressed in E. coli BL21(DE3) and purified by affinity chromatography. Functional analysis of DnaK was performed using determination of kinetics, docking, assay of chaperone activity and microbial growth.

Results: The recombinant DnaK was rapidly purified by affinity chromatography with the purification fold of 1.9 and the recovery rate of 57.9%. Km, Vmax and Kcat for Dnak from R. ruber SD3 were 80.8 μM, 58.1 nmol/min and 374.3 S-1, respectively. The recombinant protein formed trimer in vitro, with the calculated molecular weight of 214 kDa. According to In-silico analysis, DnaK interacted with other molecular chaperones and some important proteins in the metabolism. The specific activity of catalase in the presence of recombinant DnaK was 1.85 times or 2.00 times that in the presence of BSA or Tris-HCl buffer after exposure to 54 °C for 1h. E. coli transformant with pET28-dnak showed higher growth than E. coli transformant with pET28 at 43°C and in the presence of phenol, respectively.

Conclusion: The biochemical properties and the interaction analysis of DnaK from R. ruber SD3 deepened our understanding of DnaK function. DnaK played an important role in microbial growth when R. ruber was subjected to various stress such as heating and organic solvent.
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http://dx.doi.org/10.2174/0929866528666210301150421DOI Listing
March 2021

Behavior and mechanism of different fraction lead leach with several typical sulfate lixiviants in the weathered crust elution-deposited rare earth ore.

Environ Sci Pollut Res Int 2021 Jun 22;28(24):31885-31894. Epub 2021 Feb 22.

National Research Center for Geoanalysis, Beijing, 100037, China.

Recently, some new leaching agents without ammonium, such as magnesium sulfate (MgSO) and aluminum sulfate [Al(SO)], have been developed to eliminate ammonia nitrogen pollution in in situ mining process of the weathered crust elution-deposited rare earth ore (WCED-REO), but they might cause heavy metal contamination. In this study, characteristics and mechanisms of different fractions of lead (Pb) released by (NH)SO, MgSO and Al(SO) leaching agents were investigated using batch experiments and column leaching tests. The experimental results showed that the amounts of Pb released by the different leaching agents followed the trend of Al(SO) > (NH)SO > MgSO under the same total cationic charge, and both the acid extractable and reducible fractions of Pb were released. The release of acid extractable fraction Pb was related to the cation hydration radius of NH, Mg, and Al, whereas the release of reducible fraction Pb was mainly influenced by the concentration of H, especially at pH < 4.0. Furthermore, column leaching tests indicated that pH has little effect on the Pb contents of different fractions released by (NH)SO and MgSO in leaching the WCED-REO. Although Al(SO) released the largest contents of rare earth and Pb in leachate, the content of residual acid extractable fraction Pb in soil was the most after water injection (simulating the cleaning process after mining). This work can provide a scientific method and theoretical basis for comprehensively assessing the environmental impact of new leaching agents on WCED-REO mining.
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http://dx.doi.org/10.1007/s11356-021-13039-wDOI Listing
June 2021

Tug-of-War-Inspired Bio-Based Air Filters with Advanced Filtration Performance.

ACS Appl Mater Interfaces 2021 Feb 10;13(7):8736-8744. Epub 2021 Feb 10.

School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, United States.

Integrating nanostructured active materials, antimicrobial components, and rational porous structures is one of the promising approaches for simultaneously boosting removal efficiency, antimicrobial capacity, mechanical property, hydrophobic performance, and air permeability of air filters. However, realizing these performances of an air filter still remains a big challenge. Herein, a multifunctional air filter [email protected], which is composed of a unique substrate prepared from Ag nanoparticles (AgNPs)-paper towel (PT) microfibers and an upper layer formed from aligned zein nanofibers (zNFs) inspired by a "tug-of-war" repulsion force, is reported. The [email protected] substrate is fabricated via in situ reduction; and zNFs are prepared by electrospinning a well-prepared zein Pickering emulsion onto a specially designed collector. The innovative collector is a partially conductive design composed of an insulative middle section and two conductive ends. It is demonstrated that the introduction of AgNPs not only endows the [email protected] filter with an effective antimicrobial activity but also provides the substrate with an anisotropic electric field to achieve stretched and aligned zein fibers forming thinner nanofibers than that without AgNPs. As a result, the filtration performances of a [email protected] filter are enhanced. This study initiates an effective way to fabricate bio-based multifunctional air filters with antimicrobial and filtration performances via combining nano- and biotechnology.
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http://dx.doi.org/10.1021/acsami.0c20596DOI Listing
February 2021

Balanced strength-toughness, thermal conductivity and self-cleaning properties of PMMA composites enabled by terpolymer grafted carbon nanotube.

Nanotechnology 2021 May;32(19):195709

College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, People's Republic of China. School of Chemistry and Chemical Engineering, State Key Laboratory for Metal Matrix Composite Materials, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

Achieving a balanced strength-toughness in polymer composites is a challenge largely because of poor interfacial interaction between the fillers and matrix. Here, we report that terpolymer grafted multi-wall carbon nanotubes (Ter-CNT) imparted good dispersion of CNT in matrix and strong CNT-matrix interaction. With the addition of 2 vol% filler into polymethyl methacrylate (PMMA) matrix, the composite exhibited simultaneously a balanced strength-toughness property with flexural strength of 72.3 MPa, toughness of 10.1 MJ m, which increased by 40.1% and 578% compared with those of pure PMMA. In addition, the composite also shows a high static contact angle (110.3°), and thermal conductivity (0.50 Wm K), which endow the composite with good self-cleaning and thermal management capabilities. Thus, this preparation process shows guidance for the design of polymer composite with integrated high strength-toughness, thermal conductivity and good self-cleaning.
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http://dx.doi.org/10.1088/1361-6528/abe2caDOI Listing
May 2021

Effects of Taijiquan on glucose and lipid metabolism in middle-aged and elderly diabetic patients: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jan;100(4):e24433

School of Physical Education, Hubei Minzu University, Enshi, China.

Background: The improvement effect of exercise on diabetes mellitus has been widely recognized. Taijiquan, as a popular exercise mode for middle-aged and elderly people, is not clear about its effect on glucose and lipid metabolism in elderly diabetic patients. In this paper, the influence of Taijiquan on glucose and lipid metabolism in middle-aged and elderly diabetic patients was studied by using a meta-analysis method, to provide evidence for the clinical promotion of Taijiquan to improve glucose and lipid metabolism in diabetic patients.

Methods: Computer system search and manual search were conducted respectively in web of science, PubMed, Cochrane Library, EMBASE, Google Scholar, China National Knowledge Infrastructure, Wanfang Database, VIP from the inception to August 1, 2020. Randomized controlled trials of the application of Taijiquan in middle-aged and elderly diabetic patients were collected.

Results: The current study is a systematic review and meta-analysis program with no results. Data analysis will be completed after the program.

Conclusion: This review aims to study the effect of Taijiquan on the glucose and lipid metabolism of middle-aged and elderly diabetic patients, objectively evaluate the effect of Taijiquan on the glucose and lipid metabolism of middle-aged and elderly diabetic patients, and provide scientific basis for clinical exercise intervention in middle-aged and elderly diabetic patients.

Protocol Registration Number: INPLASY2020120107.
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http://dx.doi.org/10.1097/MD.0000000000024433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850770PMC
January 2021

Systematic analysis using a bioinformatics strategy identifies SFTA1P and LINC00519 as potential prognostic biomarkers for lung squamous cell carcinoma.

Am J Transl Res 2021 15;13(1):168-182. Epub 2021 Jan 15.

Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200072, China.

Lung cancer has high incidence and mortality rates, in which lung squamous cell carcinoma (LUSC) is a primary type of non-small cell lung carcinoma (NSCLC). The aim of our study was to discover long non-coding RNAs (lncRNAs) associated with diagnose and prognosis for LUSC. RNA sequencing data obtained from LUSC samples were extracted from The Cancer Genome Atlas database (TCGA). Two prognosis-associated lncRNAs (including SFTA1P and LINC00519) were selected from LUSC samples, and the expression levels were also verified to be associated abnormal in LUSC clinical samples. Our findings demonstrate that lncRNAs SFTA1P and LINC00519 exert important functions in human LUSC and may serve as new targets for LUSC diagnosis and therapy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847518PMC
January 2021

Texture Analysis of F-FDG PET/CT for Differential Diagnosis Spinal Metastases.

Front Med (Lausanne) 2020 15;7:605746. Epub 2021 Jan 15.

Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

To evaluate the value of texture analysis for the differential diagnosis of spinal metastases and to improve the diagnostic performance of 2-deoxy-2-[fluorine-18]fluoro-D-glucose positron emission tomography/computed tomography (F-FDG PET/CT) for spinal metastases. This retrospective analysis of patients who underwent PET/CT between December 2015 and January 2020 at Shanghai Tenth People's Hospital due to high FDG uptake lesions in the spine included 45 cases of spinal metastases and 44 cases of benign high FDG uptake lesions in the spine. The patients were randomly divided into a training group of 65 and a test group of 24. Seventy-two PET texture features were extracted from each lesion, and the Mann-Whitney -test was used to screen the training set for texture parameters that differed between the two groups in the presence or absence of spinal metastases. Then, the diagnostic performance of the texture parameters was screened out by receiver operating characteristic (ROC) curve analysis. Texture parameters with higher area under the curve (AUC) values than maximum standardized uptake values (SUVmax) were selected to construct classification models using logistic regression, support vector machines, and decision trees. The probability output of the model with high classification accuracy in the training set was used to compare the diagnostic performance of the classification model and SUVmax using the ROC curve. For all patients with spinal metastases, survival analysis was performed using the Kaplan-Meier method and Cox regression. There were 51 texture parameters that differed meaningfully between benign and malignant lesions, of which four had higher AUC than SUVmax. The texture parameters were input to build a classification model using logistic regression, support vector machine, and decision tree. The accuracy of classification was 87.5, 83.34, and 75%, respectively. The accuracy of the manual diagnosis was 84.27%. Single-factor survival analysis using the Kaplan-Meier method showed that intensity was correlated with patient survival. Partial texture features showed higher diagnostic value for spinal metastases than SUVmax. The machine learning part of the model combined with the texture parameters was more accurate than manual diagnosis. Therefore, texture analysis may be useful to assist in the diagnosis of spinal metastases.
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http://dx.doi.org/10.3389/fmed.2020.605746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843930PMC
January 2021

Underexposed Image Correction via Hybrid Priors Navigated Deep Propagation.

IEEE Trans Neural Netw Learn Syst 2021 Jan 29;PP. Epub 2021 Jan 29.

Enhancing visual quality for underexposed images is an extensively concerning task that plays an important role in various areas of multimedia and computer vision. Most existing methods often fail to generate high-quality results with appropriate luminance and abundant details. To address these issues, we develop a novel framework, integrating both knowledge from physical principles and implicit distributions from data to address underexposed image correction. More concretely, we propose a new perspective to formulate this task as an energy-inspired model with advanced hybrid priors. A propagation procedure navigated by the hybrid priors is well designed for simultaneously propagating the reflectance and illumination toward desired results. We conduct extensive experiments to verify the necessity of integrating both underlying principles (i.e., with knowledge) and distributions (i.e., from data) as navigated deep propagation. Plenty of experimental results of underexposed image correction demonstrate that our proposed method performs favorably against the state-of-the-art methods on both subjective and objective assessments. In addition, we execute the task of face detection to further verify the naturalness and practical value of underexposed image correction. What is more, we apply our method to solve single-image haze removal whose experimental results further demonstrate our superiorities.
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http://dx.doi.org/10.1109/TNNLS.2021.3052903DOI Listing
January 2021