Publications by authors named "Xin Deng"

437 Publications

ECF Sigma Factor HxuI Is Critical for Fitness of during Infection.

Microbiol Spectr 2022 Jan 19:e0162021. Epub 2022 Jan 19.

State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai Universitygrid.216938.7, Tianjin, China.

The opportunistic pathogen Pseudomonas aeruginosa often adapts to its host environment and causes recurrent nosocomial infections. The extracytoplasmic function (ECF) sigma factor enables bacteria to alter their gene expression in response to host environmental stimuli. Here, we report an ECF sigma factor, HxuI, which is rapidly induced once P. aeruginosa encounters the host. Host stresses such as iron limitation, oxidative stress, low oxygen, and nitric oxide induce the expression of . By combining RNA-seq and promoter- reporter fusion analysis, we reveal that HxuI can activate the expression of diverse metabolic and virulence pathways which are critical to P. aeruginosa infections, including iron acquisition, denitrification, pyocyanin synthesis, and bacteriocin production. Most importantly, overexpression of the in the laboratory strain PAO1 promotes its colonization in both murine lung and subcutaneous infections. Together, our findings show that HxuI, a key player in host stress-response, controls the adaptability and virulence of P. aeruginosa during infection. P. aeruginosa has a strong ability to adapt to diverse environments, making it capable of causing recurrent and multisite infections in clinics. Understanding host adaptive mechanisms plays an important guiding role in the development of new anti-infective agents. Here, we demonstrate that an ECFσ factor of P. aeruginosa response to the host-inflicted stresses, which promotes the bacterial fitness and pathogenicity. Furthermore, our findings may help explain the emergence of highly transmissible strains of P. aeruginosa and the acute exacerbations during chronic infections.
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http://dx.doi.org/10.1128/spectrum.01620-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8768829PMC
January 2022

Multi-Modal Convolutional Dictionary Learning.

IEEE Trans Image Process 2022 25;31:1325-1339. Epub 2022 Jan 25.

Convolutional dictionary learning has become increasingly popular in signal and image processing for its ability to overcome the limitations of traditional patch-based dictionary learning. Although most studies on convolutional dictionary learning mainly focus on the unimodal case, real-world image processing tasks usually involve images from multiple modalities, e.g., visible and near-infrared (NIR) images. Thus, it is necessary to explore convolutional dictionary learning across different modalities. In this paper, we propose a novel multi-modal convolutional dictionary learning algorithm, which efficiently correlates different image modalities and fully considers neighborhood information at the image level. In this model, each modality is represented by two convolutional dictionaries, in which one dictionary is for common feature representation and the other is for unique feature representation. The model is constrained by the requirement that the convolutional sparse representations (CSRs) for the common features should be the same across different modalities, considering that these images are captured from the same scene. We propose a new training method based on the alternating direction method of multipliers (ADMM) to alternatively learn the common and unique dictionaries in the discrete Fourier transform (DFT) domain. We show that our model converges in less than 20 iterations between the convolutional dictionary updating and the CSRs calculation. The effectiveness of the proposed dictionary learning algorithm is demonstrated on various multimodal image processing tasks, achieves better performance than both dictionary learning methods and deep learning based methods with limited training data.
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http://dx.doi.org/10.1109/TIP.2022.3141251DOI Listing
January 2022

DeepMIH: Deep Invertible Network for Multiple Image Hiding.

IEEE Trans Pattern Anal Mach Intell 2022 Jan 10;PP. Epub 2022 Jan 10.

Multiple image hiding aims to hide multiple secret images into a single cover image, and then recover all secret images perfectly. Such high-capacity hiding may easily lead to contour shadows or color distortion, which makes multiple image hiding a very challenging task. In this paper, we propose a novel multiple image hiding framework based on invertible neural network, namely DeepMIH. Specifically, we develop an invertible hiding neural network (IHNN) to innovatively model the image concealing and revealing as its forward and backward processes, making them fully coupled and reversible. The IHNN is highly flexible, which can be cascaded as many times as required to achieve the hiding of multiple images. To enhance the invisibility, we design an importance map (IM) module to guide the current image hiding based on the previous image hiding results. In addition, we find that the image hidden in the high-frequency sub-bands tends to achieve better hiding performance, and thus propose a low-frequency wavelet loss to constrain that no secret information is hidden in the low-frequency sub-bands. Experimental results show that our DeepMIH significantly outperforms other state-of-the-art methods, in terms of hiding invisibility, security and recovery accuracy on a variety of datasets.
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http://dx.doi.org/10.1109/TPAMI.2022.3141725DOI Listing
January 2022

Effectiveness of a Constructed Wetland with Carbon Filtration in Reducing Pesticides Associated with Agricultural Runoff.

Arch Environ Contam Toxicol 2022 Jan 5. Epub 2022 Jan 5.

Marine Pollution Studies Laboratory at Granite Canyon, Department of Environmental Toxicology, University of California, Davis, Monterey, CA, USA.

The Salinas Valley in Monterey County, California, USA, is a highly productive agricultural region. Irrigation runoff containing pesticides at concentrations toxic to aquatic organisms poses a threat to aquatic ecosystems within local watersheds. This study monitored the effectiveness of a constructed wetland treatment system with a granulated activated carbon (GAC) filter installation at reducing pesticide concentrations and associated toxicity to Ceriodaphnia dubia, Hyalella azteca, and Chironomus dilutus. The wetland was supplied with water pumped from an impaired agricultural and urban drainage. Across five monitoring trials, the integrated system's average pesticide concentration reduction was 52%. The wetland channel and GAC filtration components individually provided significant treatment, and within each, pesticide solubility had a significant effect on changes in pesticide concentrations. The integrated treatment system also reduced nitrate by 61%, phosphate by 73%, and turbidity by 90%. Input water was significantly toxic to C. dubia and H. azteca in the first trial. Toxicity to C. dubia persisted throughout the system, whereas toxicity to H. azteca was removed by the channel, but there was residual toxicity post-GAC. The final trial had significant input toxicity to H. azteca and C. dilutus. The channel reduced toxicity to H. azteca and removed toxicity to C. dilutus. GAC filtration reduced H. azteca toxicity to an insignificant level. There was no input toxicity in the other three trials. The results demonstrate that a wetland treatment system coupled with GAC filtration can reduce pesticide concentrations, nutrients, suspended particles, and aquatic toxicity associated with agricultural runoff.
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http://dx.doi.org/10.1007/s00244-021-00909-0DOI Listing
January 2022

Combined Intestinal Metabolomics and Microbiota Analysis for Acute Endometritis Induced by Lipopolysaccharide in Mice.

Front Cell Infect Microbiol 2021 17;11:791373. Epub 2021 Dec 17.

College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, China.

Endometritis is generally caused by bacterial infections, including both acute and chronic infections. In the past few decades, accumulated evidence showed that the occurrence of diseases might be related to gut microbiota. The progression of diseases is previously known to change the composition and diversity of intestinal microbiota. Additionally, it also causes corresponding changes in metabolites, primarily by affecting the physiological processes of microbiota. However, the effects of acute endometritis on intestinal microbiota and its metabolism remain unknown. Thus, the present study aimed to assess the effects of acute endometritis on intestinal microbes and their metabolites. Briefly, endometritis was induced in 30 specific pathogen-free (SPF) BALB/c female mice intrauterine administration of lipopolysaccharide (LPS) after anesthesia. Following this, 16S rRNA gene sequencing and liquid chromatogram-mass spectrometry (LC-MS) were performed. At the genus level, the relative abundance of , , and was found to be greater in the LPS group than in the control group. Importantly, the control group exhibited a higher ratio of and . Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids. The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community. Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.
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http://dx.doi.org/10.3389/fcimb.2021.791373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718680PMC
December 2021

3D Carbiyne Nanospheres Boosting Excellent Lithium and Sodium Storage Performance.

Small 2022 Jan 6;18(3):e2106328. Epub 2021 Dec 6.

Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, P. R. China.

Reasonable design of electrode materials with specific morphology and structure can efficiently improve the metal ions storage and transmission properties of metal ion batteries. Here the preparation of spirobifluorene-based three-dimensional carbiyne nanosphere (SBFCY-NS) that is composed of spirobifluorene (SBF) and alkyne bonds is reported. Benefiting from the rigid spatial structure of SBF, numerous precursors are coupled through the connection of acetylene bonds, extending to form solid nanospheres. Abundant storage spaces and convenient multi-directional transmission paths for metal ions are available inside the three-dimensional (3D) carbiyne structure. Thus, SBFCY-NS is applied as efficient anode for lithium-ion battery and sodium-ion battery. The good stability of SBFCY-NS-based electrode and its improved Coulombic efficiency can be attributed to the special morphology of nanospheres, which can easily form thin and stable solid electrolyte interface film on the surface. Those results further promote the preparation of spherical carbon-based materials with abundant pores that can be applied in the field of electrodes.
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http://dx.doi.org/10.1002/smll.202106328DOI Listing
January 2022

Zeolite-Encaged Isolated Platinum Ions Enable Heterolytic Dihydrogen Activation and Selective Hydrogenations.

J Am Chem Soc 2021 Dec 2;143(49):20898-20906. Epub 2021 Dec 2.

School of Materials Science and Engineering, Nankai University, Tianjin 300350, People's Republic of China.

Understanding the unique behaviors of atomically dispersed catalysts and the origin thereof is a challenging topic. Herein, we demonstrate a facile strategy to encapsulate Pt species within Y zeolite and reveal the nature of selective hydrogenation over a [email protected] model catalyst. The unique configuration of [email protected], namely atomically dispersed Pt stabilized by the surrounding oxygen atoms of six-membered rings shared by sodalite cages and supercages, enables the exclusive heterolytic activation of dihydrogen over Pt···O units, resembling the well-known classical Lewis pairs. The charged hydrogen species, i.e., H and H, are active reagents for selective hydrogenations, and therefore, the [email protected] catalyst exhibits remarkable performance in the selective hydrogenation of α,β-unsaturated aldehydes to unsaturated alcohols and of nitroarenes to arylamines.
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http://dx.doi.org/10.1021/jacs.1c09535DOI Listing
December 2021

Comparative analysis of physiological, agronomic and transcriptional responses to drought stress in wheat local varieties from Mongolia and Northern China.

Plant Physiol Biochem 2022 Jan 17;170:23-35. Epub 2021 Nov 17.

Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. Electronic address:

Drought is one of the major abiotic stresses that threaten wheat production worldwide, especially in the Mongolian Plateau and adjacent regions. This study aims to find local wheat varieties with high yields and drought resistance at various developmental stages based on agronomic traits and drought resistance indices analysis and explore the underlining molecular mechanisms by transcriptome analysis. Our results revealed that drought stress started at the seedling stage has a greater impact on crop yields. Four types of drought responses were found among the tested varieties. Type 1 and type 2 show low tolerance to drought stress despite high or low yield in control condition, type 3 exhibits high yield under control condition but dropped significantly after drought, and type 4 displays relatively high and stable yields under control and drought conditions. Transcriptome analysis performed with the representative varieties of the four types revealed GO terms and KEGG pathways enriched among drought-triggered differential expressed genes (DEGs). A network containing 18 modules was constructed using weighted gene co-expression analysis (WGCNA). Ten modules were significantly correlated to yield by module-trait correlation, and 3 modules showed Darkhan 144 specific gene expression patterns. C2H2 zinc finger factor-recognized motifs were identified from the promoters of genes in these modules. qRT-PCR confirmed several key DEGs with specific expression patterns and physiological measurements validated the relatively low oxidative damage and high antioxidant capacity in the drought tolerant variety Dankhan 144. These findings provide an important basis for local agriculture and breeding of drought-tolerant high yield wheat varieties.
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http://dx.doi.org/10.1016/j.plaphy.2021.11.026DOI Listing
January 2022

Protective Effect of Fresh/Dry Dandelion Extracts on APAP-Overdose-Induced Acute Liver Injury.

Chin J Integr Med 2021 Nov 24. Epub 2021 Nov 24.

School of Pharmaceutical Science, South-Central University for Nationalities, Wuhan, 430074, China.

Objecitvie: To compare the liver protective activity of fresh/dried dandelion extracts against acetaminophen (APAP)-induced hepatotoxicity.

Methods: Totally 90 Kunming mice were randomly divided into 10 groups according to body weight (9 mice for each group). The mice in the normal control and model (vehicle control) groups were administered sodium carboxymethyl cellulose (CMC-Na, 0.5%) only. Administration groups were pretreated with high and low-dose dry dandelion extract (1,000 or 500 g fresh herb dried and then decocted into 120 mL solution, DDE-H and DDE-L); low-, medium- and high-dose dandelion juice (250, 500, 1,000 g/120 mL, DJ-L, DJ-M, and DJ-H); fresh dandelions evaporation juice water (120 mL, DEJW); dry dandelion extract dissolved by pure water (1 kg/120 mL, DDED-PW); dry dandelion extract dissolved by DEJW (120 g/120 mL, DDED-DEJW) by oral gavage for 7 days at the dosage of 0.5 mL solution/10 g body weight; after that, except normal control group, all other groups were intraperitonealy injected with 350 mg/kg APAP to induce liver injury. Twenty hours after APAP administration, serum and liver tissue were collected and serum alanine aminotransferase (AST), aspartate transaminase (ALT), alkaline phosphatase (AKP), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) activities were quantified by biochemical kits; tumor necrosis factor (TNF-α), interleukin (IL)-2, and IL-1 β contents in liver tissue were determined by enzyme linked immunosorbent assay kits. Histopathological changes in liver tissues were observed by hematoxylin and eosin staining; TUNEL Assay and Hoechst 33258 staining were applied for cell apoptosis evaluation. The expressions of heme oxygenase-1 (HO-1), nuclear factor erythroid-2-related factor 2 (Nrf-2), caspase-9, B-cell leukemia/lymphoma 2 (Bcl-2), Bax and p-JNK were determined by Western blot analysis.

Results: Pretreatment with fresh dandelion juice (FDJ, including DJ-L, DJ-M, DJ-H, DEJW and DDED-DEJW) significantly decreased the levels of serum ALT, AST, AKP, TNF-α and IL-1β compared with vehicle control group (P<0.05 or P<0.01). Additionally, compared with the vehicle control group, FDJ decreased the levels of hepatic MDA and restored GSH levels and SOD activity in livers (P<0.05 or P<0.01). FDJ inhibited the overexpression of pro-inflammatory factors including cyclooxygenase-2 and inducible nitric oxide synthase in the liver tissues (P<0.05 or P<0.01). Furthermore, Western blot analysis revealed that FDJ pretreatment inhibited activation of apoptotic signaling pathways via decreasing of Bax, and caspase-9 and JNK protein expression, and inhibited activation of JNK pathway (P<0.05 or P<0.01). Liver histopathological observation provided further evidence that FDJ pretreatment significantly inhibited APAP-induced hepatocyte necrosis, inflammatory cell infiltration and congestion.

Conclusions: FDJ pretreatment protects against APAP-induced hepatic injury by activating the Nrf-2/HO-1 pathway and inhibition of the intrinsic apoptosis pathway, and the effect of fresh dandelion extracts was superior to dried dandelion extracts in APAP hepatotoxicity model mice.
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http://dx.doi.org/10.1007/s11655-021-3295-8DOI Listing
November 2021

Using an invertebrate model to investigate the mechanisms of short-term memory deficits induced by food deprivation.

Behav Brain Res 2022 Feb 29;418:113646. Epub 2021 Oct 29.

Department of Life Sciences, Texas A&M University - Corpus Christi, Corpus Christi, TX 78412, USA. Electronic address:

Although prolonged food deprivation is known to cause memory deficits, the underlying mechanisms are only partially understood. In this study, we began to investigate the cellular substrates of food deprivation-induced memory impairments in the invertebrate Aplysia. Following a single trial of noxious stimuli, Aplysia concurrently express short-term sensitization (an elementary form of learning in which withdrawal reflexes are enhanced) and short-term feeding suppression for at least 15 min. Cellular correlates of sensitization and feeding suppression include increased excitability of the tail sensory neurons (TSNs) controlling the withdrawal reflexes, and decreased excitability of feeding decision-making neuron B51, respectively. Recently, 14 days of food deprivation (14DFD) was reported to break the co-expression of sensitization and feeding suppression in Aplysia without health deterioration. Specifically, under 14DFD, sensitization was completely prevented while feeding suppression was present albeit attenuated. This study explored the cellular mechanisms underlying the absent sensitization and reduced feeding suppression under 14DFD. A reduced preparation was used to evaluate the short-term cellular modifications induced by delivering an aversive training protocol in vitro. TSN excitability failed to increase following in vitro training under 14DFD, suggesting that the lack of sensitization may be a consequence of the fact that TSN excitability failed to increase. B51 excitability also failed to decrease following in vitro training, indicating that additional neurons may contribute to the conserved albeit reduced feeding suppression in 14DFD animals. This study lays the foundations for the future use of the Aplysia model system to investigate the mechanisms underlying the memory impairments induced by prolonged food deprivation.
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http://dx.doi.org/10.1016/j.bbr.2021.113646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671320PMC
February 2022

Effect of Inonotus obliquus polysaccharide on composition of the intestinal flora in mice with acute endometritis.

PLoS One 2021 5;16(11):e0259570. Epub 2021 Nov 5.

College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, China.

Inonotus obliquus Polysaccharide (IOP) is a large molecule extracted from Inonotus obliqus, a medicinal fungus, which has a wide range of biological activities and has been shown to be associated with inflammation. The purpose of this study is to investigate whether IOP can help to reduce acute endometritis by regulating intestinal flora. We observed pathological changes in mice with endometritis following treatment with IOP and evaluated changes in the levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α), and further studied the effects of IOP on the intestinal flora of endometritis mice using 16S rRNA high-throughput sequencing. The results showed that IOP improved the condition of uterine tissues and reduced the release of pro-inflammatory cytokines. Meanwhile, the 16S rRNA sequencing results showed that IOP could regulate the changes in intestinal microflora at the level of genera, possibly by changing the relative abundance of some genera.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0259570PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570517PMC
December 2021

Preparation and In Vitro/In Vivo Evaluation of Orally Disintegrating/Modified-Release Praziquantel Tablets.

Pharmaceutics 2021 Sep 27;13(10). Epub 2021 Sep 27.

College of Animal Science and Technology, Guangxi University, Nanning 530004, China.

This study was designed to develop orally disintegrating/sustained-release praziquantel (PZQ) tablets using the hot-melt extrusion (HME) technique and direct compression, and subsequently evaluate their release in in vitro and in vivo pharmacokinetics. For the extrusion process, hypromellose acetate succinate (HPMCAS)-LG was the carrier of pure PZQ, with a standard screw configuration used at an extrusion temperature of 140 °C and a screw rotation speed of 100 rpm. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), powder X-ray diffraction (PXRD) and Fourier-transform infrared spectroscopy (FTIR) were performed to characterize the extrudate. Orally disintegrating/sustained-release praziquantel tablets (PZQ ODSRTs) were prepared by direct compression after appropriate excipients were blended with the extrudate. The release amount was 5.10% in pH 1.0 hydrochloric acid at 2 h and over 90% in phosphoric acid buffer at 45 min, indicating the enteric-coating character of PZQ ODSRTs. Compared with the pharmacokinetics of marketed PZQ tablets (Aipuruike) in dogs, the times to peak (T), elimination half-life (t) and mean residence time (MRT) were extended in PZQ ODSRTs, and the relative bioavailability of PZQ ODSRTs was up to 184.48% of that of Aipuruike. This study suggested that PZQ ODSRTs may have potential for the clinical treatment of parasitosis.
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http://dx.doi.org/10.3390/pharmaceutics13101567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538324PMC
September 2021

Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy.

Membranes (Basel) 2021 Sep 29;11(10). Epub 2021 Sep 29.

Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

Local inhomogeneities in lipid composition play a crucial role in the regulation of signal transduction and membrane traffic. This is particularly the case for plant plasma membrane, which is enriched in specific lipids, such as free and conjugated forms of phytosterols and typical phytosphingolipids. Nevertheless, most evidence for microdomains in cells remains indirect, and the nature of membrane inhomogeneities has been difficult to characterize. We used a new push-pull pyrene probe and fluorescence lifetime imaging microscopy (FLIM) combined with all-atom multiscale molecular dynamics simulations to provide a detailed view on the interaction between phospholipids and phytosterol and the effect of modulating cellular phytosterols on membrane-associated microdomains and phase separation formation. Our understanding of the organization principles of biomembranes is limited mainly by the challenge to measure distributions and interactions of lipids and proteins within the complex environment of living cells. Comparing phospholipids/phytosterol compositions typical of liquid-disordered (Ld) and liquid-ordered (Lo) domains, we furthermore show that phytosterols play crucial roles in membrane homeostasis. The simulation work highlights how state-of-the-art modeling alleviates some of the prior concerns and how unrefuted discoveries can be made through a computational microscope. Altogether, our results support the role of phytosterols in the lateral structuring of the PM of plant cells and suggest that they are key compounds for the formation of plant PM microdomains and the lipid-ordered phase.
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http://dx.doi.org/10.3390/membranes11100747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539253PMC
September 2021

Troponin T Elevation After Percutaneous Left Atrial Appendage Occlusion.

Front Cardiovasc Med 2021 1;8:721224. Epub 2021 Oct 1.

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Institute of Biomedical Science, Fudan University, Shanghai, China.

Cardiac troponin T (cTNT) has been widely used in detecting cardiac damage. Elevated cTNT level has been reported to be associated with increased mortality in multiple cardiac conditions. It is not uncommon to observe an increased level of cTNT in patients after left atrial appendage occlusion (LAAO). The objective of the study is to study the incidence, significance, and factors associated with cTNT elevation after LAAO. We prospectively included patients who underwent LAAO from January 2019 to July 2020 in Fudan Zhongshan Hospital. Patients were divided into those with elevated cTNT after procedure and those with normal postprocedure cTNT. All individuals were followed up for 1 year. The primary outcome is major adverse cardiovascular events, which include myocardial infarction, heart failure, cardiac death, and stroke. The second outcome is periprocedure complication, including chest pain, tachycardia, cardiac tamponade, change of electrocardiograph, and atrial thrombus. A total of 190 patients were enrolled. Of the patients, 85.3% had elevated cTNT after LAAO, while 14.7% of them did not. Exposure time, dosage of contrast, types of devices, shapes, and sizes of LAA could contribute to elevated postprocedure cTNT. We found that patients with a Watchman device were more likely to have elevated postprocedure cTNT than those with a Lambre device (89.2 vs. 76.7%, = 0.029). LAAO shapes were associated with cTNT levels in patients with a Watchman device, while the diameter of the outer disc and LAA depth mattered for the Lambre device. There was no significant difference in the primary and second outcome between the two groups (-value: 0.619, 0.674). LAAO was found to be commonly accompanied with cTNT elevation, which might not to be related to the complications and adverse cardiac outcomes within 1 year of follow-up. Moreover, eGFR at baseline, exposure time, dosage of contrast, types of LAAO device, and LAA morphology could contribute to cTNT elevation.
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http://dx.doi.org/10.3389/fcvm.2021.721224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517138PMC
October 2021

Agricultural surface water, imidacloprid, and chlorantraniliprole result in altered gene expression and receptor activation in Pimephales promelas.

Sci Total Environ 2022 Feb 13;806(Pt 4):150920. Epub 2021 Oct 13.

Department of Biological Sciences, California State University Long Beach, CA, USA. Electronic address:

The toxicity of single pesticides is likely underestimated when considering complex pesticide mixtures found in agricultural runoff and this is especially true for newer pesticides with little toxicity data on non-target species. The goal of our study was to compare the toxicity of two newer pesticides, imidacloprid (IMI) and chlorantraniliprole (CHL), when an invertebrate and fish were exposed to single compounds, binary mixtures or surface water collected near agricultural fields. A secondary goal was to determine whether changes in select subcellular molecular pathways correspond to the insecticides' mechanisms of activity in aquatic organisms. We conducted acute (96 h) exposures using a dilution series of field water and environmentally relevant concentrations of single and binary mixtures of IMI and CHL. We then evaluated survival, gene expression and the activity of IMI toward the n-acetylcholine receptor (nAChR) and CHL activity toward the ryanodine receptor (RyR). Both IMI and CHL were detected at all sampling locations for May 2019 and September 2019 sampling dates and exposure to field water led to high invertebrate but not fish mortality. Fish exposed to field collected water had significant changes in the relative expression of genes involved with detoxification and neuromuscular function. Exposure of fish to single compounds or binary mixtures of IMI and CHL led to increased relative gene expression of RyR in fish. Furthermore, we found that IMI targets the nAChR in aquatic invertebrates and that CHL can cause overactivation of the RyR in invertebrates and fish. Overall, our finding suggests that IMI and CHL may impact neuromuscular health in fish. Expanding monitoring efforts to include sublethal and molecular assays would allow the detection of subcellular level effects due to complex mixtures present in surface water near agricultural areas.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150920DOI Listing
February 2022

Double π-Extended Helicene Derivatives Containing Pentagonal Rings: Synthesis, Crystal Analyses, and Photophysics.

J Org Chem 2021 Dec 13;86(24):17535-17542. Epub 2021 Oct 13.

College of Chemistry and Environmental Science, Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding 071002, PR China.

Two novel double π-extended []helicene ( = 5, 6) derivatives ( and ) including pentagonal rings have been synthesized and characterized. Both of them have three isomers containing two enantiomers (), (), and a diastereoisomer in a meso form (,). X-ray single crystal analyses suggest that molecules and exhibit offset packing models of (,)- and (,)-isomers. Optical resolution of the resultant compound was finished, and their chiroptical properties, as well as the DFT calculations, were also examined.
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http://dx.doi.org/10.1021/acs.joc.1c00989DOI Listing
December 2021

Self-regenerable bio-hybrid with biogenic ferrous sulfide nanoparticles for treating high-concentration chromium-containing wastewater.

Water Res 2021 Nov 2;206:117731. Epub 2021 Oct 2.

CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei 230026, China; University of Science and Technology of China-City University of Hong Kong Joint Advanced Research Center, Suzhou Institute for Advance Research of USTC, Suzhou 215123, China. Electronic address:

Biogenic ferrous sulfide nanoparticles (bio-FeS) as low-cost and green-synthesized nanomaterial are promising for heavy metals removal, but the need for complicated extraction, storage processes and the production of iron sludge still restrict their practical application. Here, a self-regenerable bio-hybrid consisting of bacterial cells and self-assembled bio-FeS was developed to efficiently remove chromium (Cr(VI)). A dense layer of bio-FeS was distributed on the cell surface and in the periplasmic space of Shewanella oneidensis MR-1, endowing the bacterium with good Cr(VI) tolerance and unusual activity for bio-FeS-mediated Cr(VI) reduction. An artificial transmembrane electron channel was constituted by the bio-FeS to facilitate extracellular electron pumping, enabling efficient regeneration of extracellular bio-FeS for continuous Cr(VI) reduction. The bio-hybrid maintained high activity within three consecutive treatment-regeneration cycles for treating both simulated Cr(VI)-containing wastewater (50 mg/L) and real electroplating wastewater. Importantly, its activity can be facilely and fully restored through bio-FeS re-synthesis or regeneration with replenished fresh bacteria. Overall, the bio-hybrid merges the self-regeneration ability of bacteria with high activity of bio-FeS , opening a promising new avenue for sustainable treatment of heavy metal- containing wastewater.
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http://dx.doi.org/10.1016/j.watres.2021.117731DOI Listing
November 2021

Roles of melatonin in the field of reproductive medicine.

Biomed Pharmacother 2021 Dec 6;144:112001. Epub 2021 Oct 6.

Center Laboratory of the Fourth Affiliated Hospital, China Medical University (CMU), Shenyang, 110032, China. Electronic address:

Melatonin, mostly released by the pineal gland, is a circadian rhythm-regulated and multifunctional hormone. Great advances in melatonin research have been made, including its role in rhythms of the sleep-wake cycle, retardation of ageing processes, as well as antioxidant or anti-inflammatory functions. Melatonin can scavenge free radicals such as reactive oxygen species (ROS), a key factor in reproductive functions. Melatonin plays an important role in oocyte maturation, fertilization and embryonic development as well. The concurrent use of melatonin increases the number of mature oocytes, the fertilization rate, and number of high-quality embryos, which improves the clinical outcome of assisted reproductive technology (ART). This review discusses the relationship between melatonin and human reproductive function, and potential clinical applications of melatonin in the field of reproductive medicine.
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http://dx.doi.org/10.1016/j.biopha.2021.112001DOI Listing
December 2021

Endoscopic Endoluminal Radiofrequency Ablation and Single-Operator Peroral Cholangioscopy System (SpyGlass) in the Diagnosis and Treatment of Intraductal Papillary Neoplasm of the Bile Duct: A Case Report and Literature Review.

Front Med (Lausanne) 2021 16;8:675720. Epub 2021 Sep 16.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Intraductal papillary neoplasm of the bile duct (IPNB) is a rare biliary benign tumor with atypical clinical features and is frequently misdiagnosed. Its treatment is limited and surgical resection is thought to be the only therapeutic option in patients with IPNB. With the aim of increasing the early diagnosis rate of IPNB and providing more therapeutic options for surgeons, we innovatively put forward the concept of combined utilization of SpyGlass and endoscopic endoluminal radiofrequency ablation (ERFA) in the diagnosis and treatment of IPNB. An 85-year-old woman was referred to our hospital due to right upper quadrant abdominal pain. The image examinations indicated suspicious filling defects at the upper common bile duct. Further evaluation of SpyGlass cholangioscopy showed multiple reddish villous lesions at the left hepatic duct, and SpyBite biopsy under direct visualization demonstrated papillary low-grade dysplasia. In consideration of the advanced age and preference of the patient, the novel ERFA therapy was performed. The procedure was successful without periprocedural complications; the patient recovered uneventfully and was discharged 2 days after the operation. Upon follow-up, the patient was asymptomatic and in good physical condition at 8 months postoperatively. Preliminarily, we demonstrate that the strategy of a combination of SpyGlass and ERAF seems to be a promising, feasible, well-tolerated, and safe management for patients with IPNB. However, more data with larger patient volumes are needed to evaluate its outcomes further.
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http://dx.doi.org/10.3389/fmed.2021.675720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481375PMC
September 2021

Hierarchical Bayesian LSTM for Head Trajectory Prediction on Omnidirectional Images.

IEEE Trans Pattern Anal Mach Intell 2021 Oct 1;PP. Epub 2021 Oct 1.

When viewing omnidirectional images (ODIs), viewers can access different viewports via head movement (HM), which sequentially forms head trajectories in spatial-temporal domain. Thus, head trajectories play a key role in modeling human attention on ODIs. In this paper, we establish a large-scale dataset collecting 21,600 head trajectories on 1,080 ODIs. By mining our dataset, we find two important factors influencing head trajectories, i.e., temporal dependency and subject-specific variance. Accordingly, we propose a novel approach integrating hierarchical Bayesian inference into long short-term memory (LSTM) network for head trajectory prediction on ODIs, which is called HiBayes-LSTM. In HiBayes-LSTM, we develop a mechanism of Future Intention Estimation (FIE), which captures the temporal correlations from previous, current and estimated future information, for predicting viewport transition. Additionally, a training scheme called Hierarchical Bayesian inference (HBI) is developed for modeling inter-subject uncertainty in HiBayes-LSTM. For HBI, we introduce a joint Gaussian distribution in a hierarchy, to approximate the posterior distribution over network weights. By sampling subject-specific weights from the approximated posterior distribution, our HiBayes-LSTM approach can yield diverse viewport transition among different subjects and obtain multiple head trajectories. Extensive experiments validate that our HiBayes-LSTM approach significantly outperforms 9 state-of-the-art approaches for trajectory prediction on ODIs, and then it is successfully applied to predict saliency on ODIs.
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http://dx.doi.org/10.1109/TPAMI.2021.3117019DOI Listing
October 2021

Pseudomonas syringae senses polyphenols via phosphorelay crosstalk to inhibit virulence.

EMBO Rep 2021 Dec 28;22(12):e52805. Epub 2021 Sep 28.

Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China.

Bacteria use a variety of mechanisms, such as two-component regulatory systems (TCSs), to rapidly sense and respond to distinct conditions and signals in their host organisms. For example, a type III secretion system (T3SS) is a key determinant of the virulence of the model plant pathogen Pseudomonas syringae and contains the TCS RhpRS as a key regulator. However, the plant-derived compound targeting RhpRS remains unknown. Here, we report that RhpRS directly interacts with polyphenols and responds by switching off P. syringae T3SS via crosstalk with alternative histidine kinases. We identify three natural polyphenols that induce the expression of the rhpRS operon in an RhpS-dependent manner. The presence of these three specific polyphenols inhibits the phosphatase activity of RhpS, thus suppressing T3SS activation in T3SS-inducing conditions. The Pro40 residue of RhpS is essential to respond to these polyphenols. In addition, three non-cognate histidine kinases cooperatively phosphorylate RhpR and antagonize the rhpS mutant phenotype. This work illustrates that plant polyphenols can directly target P. syringae RhpRS, which results in bacterial virulence being switched off via a phosphorylation-related crosstalk.
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http://dx.doi.org/10.15252/embr.202152805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8647011PMC
December 2021

Development and validation of a predictive model for feeding intolerance in intensive care unit patients with sepsis.

Saudi J Gastroenterol 2021 Sep 14. Epub 2021 Sep 14.

Department of Intensive Care Medicine, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.

Background: Feeding intolerance in patients with sepsis is associated with a lower enteral nutrition (EN) intake and worse clinical outcomes. The aim of this study was to develop and validate a predictive model for enteral feeding intolerance in the intensive care unit patients with sepsis.

Methods: In this dual-center, retrospective, case-control study, a total of 195 intensive care unit patients with sepsis were enrolled from June 2018 to June 2020. Data of 124 patients for 27 clinical indicators from one hospital were used to train the model, and data from 71 patients from another hospital were used to assess the external predictive performance. The predictive models included logistic regression, naive Bayesian, random forest, gradient boosting tree, and deep learning (multilayer artificial neural network) models.

Results: Eighty-six (44.1%) patients were diagnosed with enteral feeding intolerance. The deep learning model achieved the best performance, with areas under the receiver operating characteristic curve of 0.82 (95% confidence interval = 0.74-0.90) and 0.79 (95% confidence interval = 0.68-0.89) in the training and external sets, respectively. The deep learning model showed good calibration; based on the decision curve analysis, the model's clinical benefit was considered useful. Lower respiratory tract infection was the most important contributing factor, followed by peptide EN and shock.

Conclusions: The new prediction model based on deep learning can effectively predict enteral feeding intolerance in intensive care unit patients with sepsis. Simple clinical information such as infection site, nutrient type, and septic shock can be useful in stratifying a septic patient's risk of EN intolerance.
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http://dx.doi.org/10.4103/sjg.sjg_286_21DOI Listing
September 2021

Optimized protocols for ChIP-seq and deletion mutant construction in .

STAR Protoc 2021 Sep 25;2(3):100776. Epub 2021 Aug 25.

Department of Biomedical Science, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China.

Chromatin immunoprecipitation sequencing (ChIP-seq) is an efficient technique to identify the binding sites of transcription factors (TFs) in both eukaryotes and prokaryotes. However, its application in bacteria is very heterogeneous. In this protocol, we optimized the methods of ChIP-seq that can be widely applied to plant pathogens. We used homologous recombination to construct pK18mobsacB- plasmid instead of restriction site ligation and replaced transconjugation with electroporation transformation in deletion mutant construction, which is more efficient and faster than previous methods. For complete details on the use and execution of this protocol, please refer to Shao et al. (2021).
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http://dx.doi.org/10.1016/j.xpro.2021.100776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406033PMC
September 2021

Acute Kidney Injury: Lessons from Pericardiectomy.

Heart Surg Forum 2021 Jul 27;24(4):E656-E661. Epub 2021 Jul 27.

Department of Cardiothoracic Surgery, The People's Hospital of Guangxi Zhuang Autonomous Region, 6 Taoyuan Road, Nanning, Guangxi, China.

Background: Acute kidney (renal) injury (AKI) is a severe and common complication that occurs in ~40% of patients undergoing cardiac surgery. AKI has been associated with increased mortality and worse prognosis. This prospective study was conducted to determine the risk factors for AKI after pericardiectomy and decrease the operative risk of mortality and morbidity.

Methods: This was a prospective, observational cohort study of patients with constrictive pericarditis undergoing pericardiectomy. All patients underwent pericardiectomy via median sternotomy. Serum creatinine was used as the diagnostic standard of AKI according to Kidney Disease Improving Global Outcomes classification. All survivors were monitored to the end date of the study.

Results: Consecutive patients (N = 92) undergoing pericardiectomy were divided into 2 groups: with AKI (n = 25) and without AKI (n = 67). The incidence of postoperative AKI was 27.2% (25/92). Hemodialysis was required for 10 patients (40%), and there were 5 operative deaths. Mortality, intubation time, time in intensive care unit, fresh-frozen plasma, and packed red cells of the group with AKI were significantly higher than those of the group without AKI. Both univariate and multivariate analyses showed that statistically significant independent predictors of AKI include intubation time, chest drainage, fresh-frozen plasma, and packed red cells. The latest follow-up data showed that 85 survivors were New York Heart Association class I (97.7%) and 2 were class II (2.3%).

Conclusions: AKI after pericardiectomy is a serious complication and contributes to significantly increased morbidity and mortality. Prevention of AKI development after cardiac surgery and optimization of pre-, peri-, and postoperative factors that can reduce AKI, therefore, contribute to a better postoperative outcome and leads to lower rates of AKI, morbidity, and mortality.
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http://dx.doi.org/10.1532/hsf.3869DOI Listing
July 2021

Fluid Balance of the Second Day Following Operation is Associated with Early Mortality and Multiorgan Failure After Pericardiectomy for Constrictive Pericarditis.

Heart Surg Forum 2021 Aug 25;24(4):E700-E708. Epub 2021 Aug 25.

Department of Cardiothoracic Surgery, The People's Hospital of Guangxi Zhuang, Nanning, Guangxi, China.

Background: The operative mortality of pericardiectomy still is high. This retrospective study was conducted to determine the risk factors of early mortality and multiorgan failure.

Methods: We retrospectively analyzed patients undergoing pericardiectomy from January 2009 to June 2020 at our hospital. Pericardiectomy was performed via sternotomy. Histopathologic studies of pericardium tissue from every patient were done. All survivors were monitored to the end date of the study.

Results: Ninety-two consecutive patients undergoing pericardiectomy for constrictive pericarditis were included in the study. Postoperatively, central venous pressure significantly decreased, and left ventricular end diastolic dimension and left ventricular ejection fractions significantly improved. The overall mortality rate was 5.4%. The common postoperative complications include acute renal injury (27.2%), and multiorgan failure (8.7%). Analyses of risk factors showed that fluid balance of the second day following operation is associated with early mortality and multiorgan failure. In this series from Guangxi, China, characteristic histopathologic features of tuberculosis (60/92, 65.2%) of pericardium were the most common histopathologic findings, and 32 patients (32/92, 34.8%) had the histopathologic findings of chronic nonspecific inflammatory changes. The functional status of the patients improved after pericardiectomy; 6 months later postoperatively 85 survivors were in class I (85/87, 97.7%) and two were in class II (2/87, 2.3%).

Conclusions: Tuberculosis is the most common cause of constrictive pericarditis in Guangxi, China. Fluid balance of the second day following operation is associated with early mortality and multiorgan failure after pericardiectomy for constrictive pericarditis in our study.
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http://dx.doi.org/10.1532/hsf.3939DOI Listing
August 2021

Recent Advances in Stimulus-Responsive Nanocarriers for Gene Therapy.

Adv Sci (Weinh) 2021 07 16;8(14):2100540. Epub 2021 May 16.

Department of Stomatology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei Province 430030 China.

Gene therapy provides a promising strategy for curing monogenetic disorders and complex diseases. However, there are challenges associated with the use of viral delivery vectors. The advent of nanomedicine represents a quantum leap in the application of gene therapy. Recent advances in stimulus-responsive nonviral nanocarriers indicate that they are efficient delivery systems for loading and unloading of therapeutic nucleic acids. Some nanocarriers are responsive to cues derived from the internal environment, such as changes in pH, redox potential, enzyme activity, reactive oxygen species, adenosine triphosphate, and hypoxia. Others are responsive to external stimulations, including temperature gradients, light irradiation, ultrasonic energy, and magnetic field. Multiple stimuli-responsive strategies have also been investigated recently for experimental gene therapy.
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http://dx.doi.org/10.1002/advs.202100540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292848PMC
July 2021

Prevalence of integrase strand transfer inhibitor (INSTIs) resistance mutations in Henan Province, China (2018-2020).

Infection 2021 Dec 19;49(6):1195-1202. Epub 2021 Jul 19.

The Sixth People's Hospital of Zhengzhou, Zhengzhou, 450000, China.

Background: Antiretroviral therapy (ART) regimens containing integrase strand transfer inhibitors (INSTIs) have become the recommended treatment for human immunodeficiency virus type 1 (HIV-1)-infected patients in the updated guidelines in China. In this study, we investigated the prevalence of acquired and transmitted INSTI-associated resistance of HIV-1 strains in the Henan Province (China) to provide guidance on the implementation of routine INSTI-associated HIV-1 genotypic resistance testing.

Methods: Serum samples from HIV-1-infected patients seeking treatment in our hospital from August 2018 to December 2020 were collected and the HIV-1 integrase gene coding sequence was amplified, sequenced and analyzed for INSTI resistance.

Results: We obtained integrase sequence data from a total of 999 HIV-1-infected patients, including 474 ART-naive patients, 438 ART-treated patients, and 87 patients with unknown treatment history. We detected INSTI resistance in 12 patients (1.2%, 12/999) of the study group, which included 9 ART-treated patients (2.05%, 9/438), with 6 being INSTI-treated (14.63%, 6/41) and 3 INSTI-naive (0.76%, 3/397) and 3 ART-naive (0.63%, 3/474) patients. The most common major resistance mutation was E138AK (0.5%, 5/999), while the most common accessory resistance mutation was E157Q (1.8%, 18/999). Phylogenetic analysis based on the HIV-1 integrase gene indicated that INSTI resistance was primarily detected in patients infected with HIV-1 subtype B.

Conclusions: In conclusion, our study reveals that INSTI resistance is observed in INSTI-treated patients, as expected, and the prevalence of INSTI resistance in ART-naive patients in Henan Province is low. However, baseline INSTI resistance testing should be considered, as the prescription of INSTI-based regimens is anticipated to increase considerably in the near future.
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http://dx.doi.org/10.1007/s15010-021-01668-9DOI Listing
December 2021

Pentagon-Containing π-Expanded Systems: Synthesis and Photophysical Properties.

J Org Chem 2021 Aug 19;86(15):9961-9969. Epub 2021 Jul 19.

College of Chemistry and Environmental Science, Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education Hebei University, Baoding 071002, P. R. China.

We have designed and synthesized three novel twistacene-modified enlarged pentagon-containing π-systems ( and ) with mismatched structures. The introduction of electron-withdrawing cyclopenta rings in the parent skeleton effectively stabilizes the electron-rich arenes. Their optoelectronic properties were studied via ultraviolet-visible (UV-vis) absorption spectra, fluorescence spectra, cyclic voltammetry, and density functional theory (DFT) calculation. In addition, chemical oxidation of the as-prepared compounds with nitrosonium hexafluoroantimonate could form the corresponding cationic radicals.
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http://dx.doi.org/10.1021/acs.joc.1c00332DOI Listing
August 2021

MG53 inhibits angiogenesis through regulating focal adhesion kinase signalling.

J Cell Mol Med 2021 08 9;25(15):7462-7471. Epub 2021 Jul 9.

Drug Discovery Research Center, Southwest Medical University, Luzhou, China.

Mitsugumin 53 (MG53), which is expressed predominantly in striated muscle, has been demonstrated to be a myokine/cardiokine secreted from striated muscle under specific conditions. The important roles of MG53 in non-striated muscle tissues have also been examined in multiple disease models. However, no previous study has implicated MG53 in the control of endothelial cell function. In order to explore the effects of MG53 on endothelial cells, human umbilical vein endothelial cells (HUVECs) were stimulated with recombinant human MG53 (rhMG53). Then, rhMG53 uptake, focal adhesion kinase (FAK)/Src/Akt/ERK1/2 signalling pathway activation, cell migration and tube formation were determined in vitro. The efficacy of rhMG53 in regulating angiogenesis was also detected in postnatal mouse retinas. The results demonstrated that rhMG53 directly entered into endothelial cells in a cholesterol-dependent manner. The uptake of rhMG53 directly bound to FAK in endothelial cells, which resulted in a significant decrease in FAK phosphorylation at Y397. Accompanied by the dephosphorylation of FAK, rhMG53 uncoupled FAK-Src interaction and reduced the phosphorylation of Src at Y416. Consequently, the activation of FAK/Src downstream signalling pathways, such as Akt and ERK1/2, was also significantly inhibited by rhMG53. Furthermore, rhMG53 remarkably decreased HUVEC migration and tube formation in vitro and postnatal mouse retinal angiogenesis in vivo. Taken together, these data indicate that rhMG53 inhibits angiogenesis through regulating FAK/Src/Akt/ERK1/2 signalling pathways. This may provide a novel molecular mechanism for the impaired angiogenesis in ischaemic diseases.
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http://dx.doi.org/10.1111/jcmm.16777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335693PMC
August 2021
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