Publications by authors named "Xiaoyuan Li"

74 Publications

A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals.

Nat Genet 2021 Apr 29. Epub 2021 Apr 29.

School of Life Science and Technology, ShanghaiTech University, Shanghai, China.

Phosphate (Pi) is essential to plant growth and crop yield. However, it remains unknown how Pi homeostasis is maintained during cereal grain filling. Here, we identified a rice grain-filling-controlling PHO1-type Pi transporter, OsPHO1;2, through map-based cloning. Pi efflux activity and its localization to the plasma membrane of seed tissues implicated a specific role for OsPHO1;2 in Pi reallocation during grain filling. Indeed, Pi over-accumulated in developing seeds of the Ospho1;2 mutant, which inhibited the activity of ADP-glucose pyrophosphorylase (AGPase), important for starch synthesis, and the grain-filling defect was alleviated by overexpression of AGPase in Ospho1;2-mutant plants. A conserved function was recognized for the maize transporter ZmPHO1;2. Importantly, ectopic overexpression of OsPHO1;2 enhanced grain yield, especially under low-Pi conditions. Collectively, we discovered a mechanism underlying Pi transport, grain filling and P-use efficiency, providing an efficient strategy for improving grain yield with minimal P-fertilizer input in cereals.
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http://dx.doi.org/10.1038/s41588-021-00855-6DOI Listing
April 2021

Association between VPS13C rs2414739 polymorphism and Parkinson's disease risk: A meta-analysis.

Neurosci Lett 2021 May 7;754:135879. Epub 2021 Apr 7.

Department of Neurology, Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address:

Objective: We aimed to estimate the role of vacuolar protein sorting 13C (VPS13C) gene single nucleotide polymorphism (SNP) rs2414739 variant in the risk of PD by meta-analysis.

Methods: Five eligible case-control studies including 2796 PD cases and 4138 health controls involved in this meta-analysis. The fixed or random effect model was selected based on the heterogeneity of the included studies which detected by I and Q tests. The association between rs2414739 polymorphism and the risk of PD was evaluated using the pooled odds ratios (OR) and 95 % confidence interval (95 %CI). Sensitivity analysis was used to test the stability of the results. Funnel plot and Begg's test were employed to verified publication bias.

Results: The results of our meta-analysis showed a significant correlation between VPS13C rs2424739 gene polymorphism and PD susceptibility in Allele model (A versus vs. G: OR = 1.14, 95 %CI = 1.05-1.23, p = 0.002), dominant model (GG + AG vs. AA: OR = 0.86, 95 %CI = 0.78-0.95, p = 0.004), heterozygote model (AG vs. AA: OR = 0.87, 95 %CI = 0.77-0.99, p = 0.04), homozygote model (GG vs. AA: OR = 0.76, 95 %CI = 0.60-0.96, p = 0.02). Surprisingly, we did not find a significant statistical difference between VPS13C rs2414739 polymorphism and PD risk in Chinese cohort in the regional stratified analysis.

Conclusions: This meta-analysis suggests that VPS13C rs2414739 polymorphism might act as a genetic predisposition factor for PD, whereas does not include Chinese population.
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http://dx.doi.org/10.1016/j.neulet.2021.135879DOI Listing
May 2021

NMR-Based Metabolomic Analysis for the Effects of α-Ketoglutarate Supplementation on C2C12 Myoblasts in Different Energy States.

Molecules 2021 Mar 25;26(7). Epub 2021 Mar 25.

Key Laboratory of Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

α-Ketoglutarate (AKG) is attracting much attention from researchers owing to its beneficial effects on anti-aging and cancer suppression, and, more recently, in nutritional supplements. Given that glucose is the main source of energy to maintain normal physiological functions of skeletal muscle, the effects of AKG supplementation for improving muscle performance are closely related to the glucose level in skeletal muscle. The differences of AKG-induced effects in skeletal muscle between two states of normal energy and energy deficiency are unclear. Furthermore, AKG-induced metabolic changes in skeletal muscles in different energy states also remain elusive. Here, we assessed the effects of AKG supplementation on mouse C2C12 myoblast cells cultured both in normal medium (Nor cells) and in low-glucose medium (Low cells), which were used to mimic two states of normal energy and energy deficiency, respectively. We further performed NMR-based metabolomic analysis to address AKG-induced metabolic changes in Nor and Low cells. AKG supplementation significantly promoted the proliferation and differentiation of cells in the two energy states through glutamine metabolism, oxidative stress, and energy metabolism. Under normal culture conditions, AKG up-regulated the intracellular glutamine level, changed the cellular energy status, and maintained the antioxidant capacity of cells. Under low-glucose culture condition, AKG served as a metabolic substrate to reduce the glutamine-dependence of cells, remarkably enhanced the antioxidant capacity of cells and significantly elevated the intracellular ATP level, thereby ensuring the normal growth and metabolism of cells in the state of energy deficiency. Our results provide a mechanistic understanding of the effects of AKG supplements on myoblasts in both normal energy and energy deficiency states. This work may be beneficial to the exploitation of AKG applications in clinical treatments and nutritional supplementations.
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http://dx.doi.org/10.3390/molecules26071841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037044PMC
March 2021

Efficacy of Shenling Baizhu San on stable chronic obstructive pulmonary disease patients: A systematic review and meta-analysis.

J Ethnopharmacol 2021 May 16;272:113927. Epub 2021 Feb 16.

Reproductive Health Center, Women and Children's Hospital, Qingdao University, Qingdao, China. Electronic address:

Ethnopharmacological Relevance: Shenling Baizhu San (SBS) as a classic Chinese medicine prescription, has been extensively used in gastrointestinal diseases, such as ulcerative colitis and chronic diarrhea. In recent years, SBS has shown a beneficial effect on chronic obstructive pulmonary disease (COPD) patients. However, clinical trials had shown conflicting results of SBS on improving pulmonary function and other related indicators of patients with stable COPD. The efficacy of SBS on stable COPD patients has not been fully assessed.

Aim Of The Study: To determine whether the SBS used in the treatment of gastrointestinal disease was effective to treat COPD, we assessed the clinical evidence and efficacy of SBS supplemental treatment on stable COPD patients by a systematic review and meta-analysis of clinical trials.

Materials And Methods: Nine electronic databases were searched to include clinical trials (published until August 31, 2020) with SBS as a supplementation treatment on stable COPD. Mean difference (MD) was used to evaluate continuous variables, odds ratio (OR) was calculated to evaluate dichotomous. The Egger's test was applied for publication bias.

Results: A total of 770 COPD participants from 11 trials that met the inclusion criteria were included. The meta-analysis showed that modified SBS could improve the exercise endurance, life quality scores of stable COPD patients, and also showed the potential benefits to pulmonary function of COPD patients than original SBS.

Conclusion: The methodological quality of included trials may limit the conclusions that indicate that modified SBS may have a promising treatment for improving FEV/FVC and MVV, increasing exercise endurance and life quality scores on stable COPD patients.
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http://dx.doi.org/10.1016/j.jep.2021.113927DOI Listing
May 2021

Green Synthesis of Porous Spherical Reduced Graphene Oxide and Its Application in Immobilized Pectinase.

ACS Omega 2020 Dec 11;5(50):32706-32714. Epub 2020 Dec 11.

Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education & Xinjiang Uyghur Autonomous Region, School of Chemical Engineering and Technology, Xinjiang University, Urumqi, Xinjiang 830046, China.

Pectinase is widely used in juice production, food processes, and other fields. However, owing to poor stability, free pectinase is difficult to separate from a substrate after hydrolysis and cannot be reused, and thus its industrial use is limited. Immobilized pectinase can solve these problems well. We prepared a carrier material of immobilized enzyme, which is called porous spherical reduced graphene oxide (rGO) with a rich pore structure, large specific surface area, strong hardness, and good biocompatibility to enzyme. Then, we evaluated the performance of the porous spherical rGO immobilized pectinase and characterized its structure by IR, XRD, and SEM. Using this material as a carrier of immobilized enzyme improves the load and catalytic activity of the enzyme. After 10 times of continuous use, the porous spherical rGO immobilized enzyme still maintained its initial relative enzyme activity at around 87%, indicating that immobilized pectinase had a stronger cycling stability, and its thermal stability, acid-base tolerance, and storage stability were superior to those of free pectinase. The results were compared with those of other studies on immobilized pectinase. The relative activity of pectinase immobilized by porous spherical rGO was at a high level after 10 consecutive uses. Overall, the spherical rGO is an excellent immobilized enzyme carrier material.
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http://dx.doi.org/10.1021/acsomega.0c05078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758952PMC
December 2020

Effects of fitness qigong and tai chi on middle-aged and elderly patients with type 2 diabetes mellitus.

PLoS One 2020 17;15(12):e0243989. Epub 2020 Dec 17.

School of Life Sciences, Zhengzhou University, Zhengzhou, China.

Currently, qigong and tai chi exercises are the two most common preventive as well as therapeutic interventions for chronic metabolic diseases such as type 2 diabetes mellitus (T2DM). However, the quantitative evaluation of these interventions is limited. This study aimed to evaluate the therapeutic efficacy of qigong and tai chi intervention in middle-aged and older adults with T2DM. The study included 103 eligible participants, who were randomized to participate for 12 weeks, in one of the following intervention groups for the treatment of T2DM: fitness qigong, tai chi, and control group. Three biochemical measures, including fasting plasma glucose (FPG), glycated hemoglobin (HbA1C), and C-peptide (C-P) levels, assessed at baseline and 12 weeks, served as the primary outcome measures. During the training process, 16 of the 103 participants dropped out. After the 12-week intervention, there were significant influences on HbA1C (F2,83 = 4.88, p = 0.010) and C-P levels (F2,83 = 3.64, p = 0.031). Moreover, significant reduction in C-P levels was observed after 12-week tai chi practice (p = 0.004). Furthermore, there was a significant negative correlation between the duration of T2DM and the relative changes in FPG levels after qigong intervention, and the relative changes in HbA1C levels were positively correlated with waist-to-height ratio after tai chi practice. Our study suggests that targeted qigong exercise might have a better interventional effect on patients with a longer duration of T2DM, while tai chi might be risky for people with central obesity. Trial registration: This trial was registered in Chinese Clinical Trial Registry. The registration number is ChiCTR180020069. The public title is "Health-care qigong · study for the prescription of chronic diabetes intervention."
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0243989PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746158PMC
February 2021

Nanotextured silk fibroin/hydroxyapatite biomimetic bilayer tough structure regulated osteogenic/chondrogenic differentiation of mesenchymal stem cells for osteochondral repair.

Cell Prolif 2020 Nov 1;53(11):e12917. Epub 2020 Oct 1.

Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.

Objectives: Articular cartilage plays a vital role in bearing and buffering. Injured cartilage and subchondral bone repair is a crucial challenge in cartilage tissue engineering due to the peculiar structure of osteochondral unit and the requirement of osteogenic/chondrogenic bi-directional differentiation. Based on the bionics principle, a nanotextured silk fibroin (SF)-chondroitin sulphate (CS)/hydroxyapatite (HAp) nanowire tough bilayer structure was prepared for osteochondral repair.

Methods: The SF-CS/HAp membrane was constructed by alcohol-induced β-sheet formation serving as the physical crosslink. Its osteochondral repairing capacity was evaluated by culturing bone marrow mesenchymal stem cells (BMSCs) in vitro and constructing a rat osteochondral defect model in vivo.

Results: The bilayer SF-CS/HAp membrane with satisfactory mechanical properties similar to natural cartilage imitated the natural osteochondral unit structural layers and exerted the function of bearing and buffering timely after in vivo implantation. SF-CS layer upregulated the expression of chondrogenesis-related genes of BMSCs by surface nanotopography and sustained release CS. Meanwhile, nanotextured HAp layer assembled with nanowire endowed the membrane with an osteogenic differentiation tendency for BMSCs. In vivo results proved that the biomimetic bilayer structure dramatically promoted new cartilage formation and subchondral bone remodelling for osteochondral defect model after implantation.

Conclusions: The SF-CS/HAp biomimetic bilayer membrane provides a promising strategy for precise osteochondral repair.
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http://dx.doi.org/10.1111/cpr.12917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653257PMC
November 2020

Grain Size Selection Using Novel Functional Markers Targeting 14 Genes in Rice.

Rice (N Y) 2020 Sep 9;13(1):63. Epub 2020 Sep 9.

Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China.

Background: Grain size is an extremely important aspect of rice breeding, affecting both grain yield and quality traits. It is controlled by multiple genes and tracking these genes in breeding schemes should expedite selection of lines with superior grain yield and quality, thus it is essential to develop robust, efficient markers.

Result: In this study, 14 genes related to grain size (GW2, GS2, qLGY3, GS3, GL3.1, TGW3, GS5, GW5, GS6, TGW6, GW6a, GLW7, GL7 and GW8) were selected for functional marker development. Twenty-one PCR-gel-based markers were developed to genotype the candidate functional nucleotide polymorphisms (FNPs) of these genes, and all markers can effectively recognize the corresponding allele types. To test the allele effects of different FNPs, a global collection of rice cultivars including 257 accessions from the Rice Diversity Panel 1 was used for allele mining, and four grain-size-related traits were investigated at two planting locations. Three FNPs for GW2, GS2 and GL3.1 were genotyped as rare alleles only found in cultivars with notably large grains, and the allele contributions of the remaining FNPs were clarified in both the indica and japonica subspecies. Significant trait contributions were found for most of the FNPs, especially GS3, GW5 and GL7. Of note, GW5 could function as a key regulator to coordinate the performance of other grain size genes. The allele effects of several FNPs were also tested by QTL analysis using an F population, and GW5 was further identified as the major locus with the largest contribution to grain width and length to width ratio.

Conclusions: The functional markers are robust for genotyping different cultivars and may facilitate the rational design of grain size to achieve a balance between grain yield and quality in future rice breeding efforts.
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http://dx.doi.org/10.1186/s12284-020-00427-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481322PMC
September 2020

Immobilization of Pectinase onto Porous Hydroxyapatite/Calcium Alginate Composite Beads for Improved Performance of Recycle.

ACS Omega 2020 Aug 6;5(32):20062-20069. Epub 2020 Aug 6.

Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education & Xinjiang Uyghur Autonomous Region, College of Chemistry and Chemical Engineering, Xinjiang Univeristy, Urumqi 830046, China.

Pectinase is an industrially important enzyme widely used in juice production, food processing, and other fields. The use of immobilized enzyme systems that allow several reuses of pectinase is beneficial to these fields. Herein, we developed mechanically strong and recyclable porous hydroxyapatite/calcium alginate composite beads for pectinase immobilization. Under the optimal immobilization parameters of 40 °C, pH 4.0, 5.2 U/L pectinase concentration and 4 h reaction time, pectinase showed the highest enzymatic activity (8995 U/mg) and immobilization yield (91%). The thermal stability and pH tolerance of the immobilized pectinase were superior to those of free pectinase. The storage stability of the free and immobilized pectinase for 30 days retained 20 and 50% of their initial activity, respectively. Therefore, these composite beads might be promising support for the efficient immobilization of industrially important enzymes.
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http://dx.doi.org/10.1021/acsomega.0c01625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439264PMC
August 2020

Association between matrix metalloproteinase 9 polymorphisms and breast cancer risk: An updated meta-analysis and trial sequential analysis.

Gene 2020 Oct 30;759:144972. Epub 2020 Jul 30.

Department of Breast Surgery, Meizhou People's Hospital, Meizhou 514000, Guangdong, China.

Background: Numerous studies have sought associations between matrix metalloproteinase 9 (MMP-9) polymorphisms and breast cancer risk. However, these studies have yielded conflicting results. Hence, we performed an updated meta-analysis to clarify the effects of four MMP-9 gene polymorphisms (rs3918242, rs2250889, rs3787268, and rs17576) on breast cancer risk.

Methods: A comprehensive literature search for eligible studies was conducted in five electronic databases, including PubMed, Embase and Web of Science, up to March 1, 2020. Summary odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of associations in random-effects models. For the reduction of type I errors, a trial sequential analysis (TSA) was performed.

Results: Twenty-one studies (8813 breast cancer cases and 9323 controls) were included in the quantitative analysis. For rs3918242, the overall ORs were significant under allelic comparison (OR  = 1.34; 95% CI 1.03, 1.74, P = 0.028) and the recessive genetic model (OR  = 1.40; 95% CI 1.06, 1.84, P = 0.016). For rs2250889, the ORs were significant under homozygote comparison (OR  = 2.57; 95% CI 1.22, 5.42, P = 0.013), heterozygote comparison (OR  = 2.48; 95% CI 1.17, 5.23, P = 0.018), and the dominant genetic model (OR  = 2.53; 95% CI 1.23, 5.20, P = 0.012). No associations were observed for rs3787268 or rs17576. The subgroup analyses indicated that the risk effect of the rs3918242 A allele was observed only among Asians. TSA showed that the findings for rs3918242, rs3787268, and rs17576 were robust, but many more patients are needed before definitive conclusions can be made for rs2250889.

Conclusion: Our meta-analysis suggests that MMP-9 rs3918242, but not rs3787268 and rs17576 polymorphisms, may be risk factors for breast cancer. The effect of rs2250889 needs further confirmation with a larger sample size.
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http://dx.doi.org/10.1016/j.gene.2020.144972DOI Listing
October 2020

Perceived quality of care and its associated factors among Chinese patients with advanced cancer: findings from the APPROACH study in Beijing.

Support Care Cancer 2021 Mar 15;29(3):1395-1401. Epub 2020 Jul 15.

Lien Centre for Palliative Care, Duke-NUS Medical School, Singapore, Singapore.

Purpose: Patient-perceived quality of care has become an increasingly important index within the healthcare setting. We examined patient-reported overall quality of care and patient experiences in three specific domains of care (physician communication, nursing care, and care coordination) in a sample of Chinese patients with advanced cancer.

Methods: A cross-sectional study was conducted with stage IV cancer patients (N = 202) who were recruited from a public, tertiary hospital in Beijing. Study participants completed surveys administered by a research assistant. Multivariable regression analysis was conducted to examine the extent to which patient demographic factors (age, gender, socioeconomic status), disease/treatment factors, and domain-specific care were associated with overall quality of care.

Results: A majority of patients reported overall quality of care scores that we were either excellent (23%) or very good (41%). Patients reported highest ratings in the domain of nursing care (M = 87.57, SD = 31.05), followed by physician communication (M = 68.93, SD = 32.30), and care coordination (M = 66.79, SD = 25.17). Better perceived physician communication (b = 0.17, p < 0.01), care coordination (b = 0.26, p < 0.01), and higher socioeconomic status (b = 11.30, p < 0.05) were associated with higher overall quality of care.

Conclusions: A majority of patients with advanced cancer in this Chinese hospital reported positive overall quality of care. Physician communication and care coordination are potential areas to focus on to improve patient-reported overall quality of care. Understanding perceptions of care quality will allow opportunities to improve delivery of healthcare.
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http://dx.doi.org/10.1007/s00520-020-05559-xDOI Listing
March 2021

Photoactivated polyprodrug nanoparticles for effective light-controlled Pt(iv) and siRNA codelivery to achieve synergistic cancer therapy.

J Mater Chem B 2020 07;8(27):5903-5911

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

Endo/lysosomal escape and the subsequent controllable/precise release of drugs and genes are key challenges for efficient synergistic cancer therapy. Herein, we report a photoactivated polyprodrug nanoparticle system (PPNPsiRNA) centered on effective light-controlled codelivery of Pt(iv) prodrug and siRNA for synergistic cancer therapy. Under green-light irradiation, PPNPsiRNA can sustainedly generate oxygen-independent azidyl radicals to facilitate endo/lysosomal escape through the photochemical internalization (PCI) mechanism. Besides, concurrent Pt(ii) release and siRNA unpacking could occur in a controllable manner after the decomposition of Pt(iv), main chain shattering of photoactivated polyprodrug and the PPNPsiRNA disassociation. Based on these innovative features, excellent synergistic therapeutic efficacy of chemo- and RNAi therapies of PPNPsiBcl-2 could be achieved on ovarian cancer cells under light irradiation. The facile synthesized and prepared photoactivatable polyprodrug nanoparticle system provides a new strategy for effective gene/drug codelivery, where controllable endo/lysosomal escape and the subsequent drug/gene release/unpacking play vital roles, which could be adopted as a versatile codelivery nanoplatform for the treatment of various cancers.
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http://dx.doi.org/10.1039/d0tb01103gDOI Listing
July 2020

Pyrazinamide alleviates rifampin-induced steatohepatitis in mice by regulating the activities of cholesterol-activated 7α-hydroxylase and lipoprotein lipase.

Eur J Pharm Sci 2020 Aug 31;151:105402. Epub 2020 May 31.

Sichuan Academy of Chinese Medicine Sciences, Chengdu, China. Electronic address:

The combination of rifampin and pyrazinamide is commonly used in the clinical treatment of tuberculosis, but its safety needs to be further clarified. Mice were intragastric administration of rifampin 300 mg/kg, pyrazinamide 625 mg/kg, rifampin 300 mg/kg plus pyrazinamide 625 mg/kg. The results showed that rifampin significantly increased transaminases, TBIL and TBA levels in serum, increased TG, TC content, HMGCR and CYP7A1 protein, CYP7A1, FGFR4, PXR, FAS and FXR mRNA expression, but decreased the level of SREBP-1c mRNA and induced severe steatohepatitis and hepatocyte necrosis in liver in mice. While pyrazinamide can improve many abnormal indexes when it used with RFP, including liver histopathology, liver TG, TC level and serum biochemistry, GPHBP1, FAS and CYP7A1 mRNA, LPL protein expression and activity induced by rifampin. However, pyrazinamide alone significantly decreased liver TG levels and caused only slight inflammatory pathological changes in liver histopathology in mice. These data suggested that rifampin increases TG and TC levels in the liver may be related to activate HMGCR, CYP7A1, PXR and FXR, theses toxic actions of rifampin were alleviated by pyrazinamide may be due to inhibite the activity of CYP7A1, PXR and FAS, and increasing the LPL protein expression and activity.
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http://dx.doi.org/10.1016/j.ejps.2020.105402DOI Listing
August 2020

Morphology tunable and acid-sensitive dextran-doxorubicin conjugate assemblies for targeted cancer therapy.

J Mater Chem B 2020 08 13;8(31):6898-6904. Epub 2020 May 13.

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

Stimuli-responsive and targetable nanomedicine systems have been widely applied as effective modalities for drug delivery and tumor therapeutics. Particle shape is also important for the biodistribution and cellular uptake in drug delivery applications. Here, morphology tunable and acid-responsive dextran-doxorubicin conjugate assemblies of DD-M and DDF-V for targeted doxorubicin (DOX) delivery were constructed, which contain the following favorable advantages: (1) one-pot synthesis of the drug loaded system with a Schiff base reaction is a green chemistry method which is better than the conventional drug conjugation/encapsulation methods. (2) The morphology of the nanoparticles could be regulated from a micelle (DD-M) to vesicle (DDF-V) structure by either introducing folic acid (FA) or not. (3) The abundant hydroxyl groups and electronegativity give DD-M and DDF-V superior stability in the physiological environment. (4) Besides, the multifunctional DDF-V with its important merits including tumor-targeting ability and acid-responsiveness is specific for DOX delivery in cancer therapy. (5) Compared to free DOX and DD-M, DDF-V displayed enhanced anti-tumor efficacy both in vitro and in vivo without obvious systematic toxicity. The morphology tunable, acid-sensitive and targetable nanosystem could be a promising strategy for site-specific drug delivery and potential cancer therapy in the future.
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http://dx.doi.org/10.1039/d0tb00746cDOI Listing
August 2020

Alpha rhythm slowing in a modified thalamo-cortico-thalamic model related with Alzheimer's disease.

PLoS One 2020 12;15(3):e0229950. Epub 2020 Mar 12.

Department of Applied Mathematics, Northwestern Polytechnical University, Xi'an, PR China.

A decrease in alpha band power is defined as a hallmark of electroencephalogram (EEG) in Alzheimer's disease (AD). This study devotes to understanding the neuronal correlates of alpha rhythm slowing associated with AD from the view of neurocomputation. Firstly, a modified computational model of thalamo-cortico-thalamic (TCT) circuitry is constructed by incorporating two important biologically plausible ingredients. One is the disinhibition property between different inhibitory interneurons in the cortical module. The other is the full relay function of thalamic relay nucleus (TCR) to the cortical module. Then, by decreasing synaptic connectivity parameters to mimic the neuropathological condition of synapse loss in AD, the correlation between neuronal synaptic behavior and abnormal alpha rhythm is simulated by means of power spectral analysis. The results indicate that these decreases of synaptic activity, i.e., not only the excitatory synaptic connections from TCR to fast inhibitory interneurons Cfte and from excitatory interneurons to pyramidal neurons Cpxe but also the inhibitory synaptic connections from fast inhibitory interneurons to slow inhibitory interneurons Clfi and from inhibitory interneurons to TCR Ctii, can significantly diminish the peak power density over the alpha band of the thalamic output, which implies that there is a slowing of alpha band. Furthermore, the underlying mechanism behind the alpha rhythmic changes is analyzed using nonlinear dynamical technique. The results reveal that decreases of Cfte, Cpxe, Clfi and Ctii can make the thalamic module transfer from a limit cycle mode to a point attractor mode, which may lead to the alpha rhythm slowing in the modified TCT model. We expect this work can be helpful in identifying early biomarkers of AD's EEG and understanding potential pathogenesis of AD.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229950PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067465PMC
June 2020

Cadmium and lead mixtures are less toxic to the Chinese medicinal plant Ligusticum chuanxiong Hort. Than either metal alone.

Ecotoxicol Environ Saf 2020 Apr 25;193:110342. Epub 2020 Feb 25.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.

Agricultural production of Ligusticum chuanxiong Hort. is often affected by heavy metal pollution in soil, especially mixtures of cadmium (Cd) and lead (Pb). We assessed metal-induced phytotoxicity in L. chuanxiong by exposing the plants to soil treated with Cd, Pb, or Cd/Pb mixtures. A combined Cd/Pb treatment alleviated the inhibition in plant growth, photosynthesis, and secondary metabolite generation seen in single-metal exposures in three of the four combinations. Most combined Cd/Pb treatments resulted in preferential uptake of magnesium, copper, and nitrogen in underground plant parts and accumulation of phosphorus and calcium in aboveground plant parts, thereby leading to improvements in photosynthetic potential. Compared with single-metal exposures, combined Cd/Pb treatment significantly decreased the contents of Cd by 16.67%-40.12% and Pb by 10.68%-21.70% in the plant, respectively. At the subcellular level, the Pb presence increased the Cd percentage associated with cell wall from 64.79% to 67.93% in rhizomes and from 32.76% to 45.32% in leaves, while Cd reduced Pb contents by 9.36%-46.39% in the subcellular fractions. A combined Cd/Pb treatment decreased the contents of water- and ethanol-extractable metal forms and increased the contents of acetic acid- and hydrochloric acid-extractable forms. The lower toxic effects of the Cd/Pb mixture in L. chuanxiong were associated with photosynthetic potential, subcellular distribution, the chemical forms of Cd and Pb, and synthesis of secondary metabolites. These findings are useful for plant production strategies in soils contaminated by heavy metals.
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http://dx.doi.org/10.1016/j.ecoenv.2020.110342DOI Listing
April 2020

DNA and RNA sequencing identified a novel oncogene VPS35 in liver hepatocellular carcinoma.

Oncogene 2020 04 19;39(16):3229-3244. Epub 2020 Feb 19.

Department of Bioinformatics, School of Basic Medicine, Chongqing Medical University, Chongqing, China.

Liver hepatocellular carcinoma (LIHC) is the second leading cause of cancer mortality worldwide. Although cancer driver genes identified so far have been considered to be saturated or nearly saturated, challenges remain in discovering novel genes underlying carcinogenesis due to significant tumor heterogeneity. Here, in a small cohort of hepatitis B virus (HBV)-associated LIHC, we investigated the transcriptional patterns of tumor-mutated alleles using both whole-exome and RNA sequencing data. A graph clustering of the transcribed tumor-mutated alleles characterized overlapped functional clusters, and thus prioritized potentially novel oncogenes. We validated the function of the potentially novel oncogenes in vitro and in vivo. We showed that a component of the retromer complex-the vacuolar protein sorting-associated protein 35 (VPS35)-promoted the proliferation of hepatoma cell through the PI3K/AKT signaling pathway. In VPS35-knockout hepatoma cells, a significantly reduced distribution of membrane fibroblast growth factor receptor 3 (FGFR3) demonstrated the effects of VPS35 on sorting and trafficking of transmembrane receptor. This study provides insight into the roles of the retromer complex on carcinogenesis and has important implications for the development of personalized therapeutic strategies for LIHC.
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http://dx.doi.org/10.1038/s41388-020-1215-6DOI Listing
April 2020

Clinicopathological features and treatment outcomes of metastatic or locally unresectable small bowel adenocarcinoma.

J BUON 2019 Nov-Dec;24(6):2539-2545

Department of Medical Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.

Purpose: Small bowel adenocarcinoma (SBA) is an uncommon malignancy with poor prognosis and therefore difficult to study. The purpose of this study was to evaluate the characteristics, treatments and prognostic factors in patients with metastatic or locally unresectable SBA.

Methods: Epidemiological and treatment data from metastatic or locally unresectable SBA patients who were admitted to Peking Union Medical College Hospital for first-line chemotherapy between December 2003 and November 2016 were retrospectively analyzed.

Results: Of the 34 enrolled patients, 22 (64.7%) were male and 12 (35.3%) female, with a median age of 52 years. Tumors originated in the duodenum in 24 (70.6%) patients. All patients received one of the following regimens as first-line therapy: FOLFOX or XELOX (n = 27), FOLFIRI or CAPIRI (n = 5), GEMOX (n = 1), and TP (n = 1). The response rate and disease control rate were 11.8 and 61.8%, respectively. The median progression-free survival (PFS) and overall survival (OS) were 4.5 and 13.8 months, respectively. Multivariate analysis revealed that liver metastasis was independently associated with poor PFS, and both unresected primary tumor and males were significantly associated with poor OS. The survival of three metastatic patients was 52-96 months after combination treatment of chemotherapy, resection of primary tumor and metastasis.

Conclusions: The prognosis of metastatic or locally unresectable SBA was poor, and unresected primary tumor and males were significantly associated with poor OS. Combined modality therapy of systemic chemotherapy combined with local treatment of the primary tumor and oligometastasis might improve prognosis in selected patients.
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June 2020

A Multi-Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy.

Macromol Biosci 2019 12 10;19(12):e1900255. Epub 2019 Nov 10.

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.

Implant materials applied in bone defect commonly focus on the inducement of bone regeneration and neglect to cure complications including bacterial infection and inflammation, which may result in delayed unions or even amputation. In this study, a microporous silica nanoparticle-poly(N-isopropylacrylamide-b-(2-(dimethylamino)ethyl methacrylate) is synthesized for loading DXMS and the ECM-derived peptide (Sequence: Succinic acid-GTPGPQGIAGQRGVV) in order to enhance the osteoblast calcification and relieve related symptoms. Positively charged PDMA blocks endow the nanoparticle with the antimicrobial property. Moreover, the combination of DXMS makes it have the ability of anti-inflammation and promoting calcification formation. Furthermore, incorporation of the peptide leads to a significant improvement of mineralization and alkaline phosphatase expression in the preosteoblast. After intramuscular implantation in mice for four weeks, the results indicate the composite nanoparticle can promote ectopic bone formation. These combined properties make the composite silicon nanoparticle a promising osteogenic drug appropriate for further study in bone repair and related combination therapy.
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http://dx.doi.org/10.1002/mabi.201900255DOI Listing
December 2019

Transcriptomic Analysis of mRNA-lncRNA-miRNA Interactions in Hepatocellular Carcinoma.

Sci Rep 2019 11 6;9(1):16096. Epub 2019 Nov 6.

Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, P.R. China.

Fully elucidating the molecular mechanisms of non-coding RNAs (ncRNAs), including micro RNAs (miRNAs) and long non-coding RNAs (lncRNAs), underlying hepatocarcinogenesis is challenging. We characterized the expression profiles of ncRNAs and constructed a regulatory mRNA-lncRNA-miRNA (MLMI) network based on transcriptome sequencing (RNA-seq) of hepatocellular carcinoma (HCC, n = 9) patients. Of the identified miRNAs (n = 203) and lncRNAs (n = 1,090), we found 16 significantly differentially expressed (DE) miRNAs and three DE lncRNAs. The DE RNAs were highly enriched in 21 functional pathways implicated in HCC (p < 0.05), including p53, MAPK, and NAFLD signaling. Potential pairwise interactions between DE ncRNAs and mRNAs were fully characterized using in silico prediction and experimentally-validated evidence. We for the first time constructed a MLMI network of reciprocal interactions for 16 miRNAs, three lncRNAs, and 253 mRNAs in HCC. The predominant role of MEG3 in the MLMI network was validated by its overexpression in vitro that the expression levels of a proportion of MEG3-targeted miRNAs and mRNAs was changed significantly. Our results suggested that the comprehensive MLMI network synergistically modulated carcinogenesis, and the crosstalk of the network provides a new avenue to accurately describe the molecular mechanisms of hepatocarcinogenesis.
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http://dx.doi.org/10.1038/s41598-019-52559-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834564PMC
November 2019

Solar and wind energy enhances drought resilience and groundwater sustainability.

Nat Commun 2019 11 6;10(1):4893. Epub 2019 Nov 6.

Department of Civil and Environmental Engineering, Princeton University, Princeton, NJ, USA.

Water scarcity brings tremendous challenges to achieving sustainable development of water resources, food, and energy security, as these sectors are often in competition, especially during drought. Overcoming these challenges requires balancing trade-offs between sectors and improving resilience to drought impacts. An under-appreciated factor in managing the water-food-energy (WFE) nexus is the increased value of solar and wind energy (SWE). Here we develop a trade-off frontier framework to quantify the water sustainability value of SWE through a case study in California. We identify development pathways that optimize the economic value of water in competition for energy and food production while ensuring sustainable use of groundwater. Our results indicate that in the long term, SWE penetration creates beneficial feedback for the WFE nexus: SWE enhances drought resilience and benefits groundwater sustainability, and in turn, maintaining groundwater at a sustainable level increases the added value of SWE to energy and food production.
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http://dx.doi.org/10.1038/s41467-019-12810-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834588PMC
November 2019

A method to visually observe the degradation-diffusion-reconstruction behavior of hydroxyapatite in the bone repair process.

Acta Biomater 2020 01 2;101:554-564. Epub 2019 Nov 2.

Department of Periodontology, School and Hospital of Stomatology, Shandong University and Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong 250012, China. Electronic address:

Nanostructured hydroxyapatite (HAp) has been applied widely as a scaffold material for bone tissue engineering for its good osteoinduction and biodegradability. However, the degradation process and the distribution of degraded HAp within the bone-defect cavity is still not clear. To visually study the behavior of HAp in bone repair process, a membrane of HAp/terbium (Tb)-HAp nanowires (NWs) was prepared with a concentric circle structure (CCS), of which the inner circle and the outer ring were constructed with Tb-HAp and HAp NWs, respectively. HAp/Tb-HAp CCS membrane possessed good osteogenic capacity and efficient fluorescence in the center for visualization. The in vitro experimental results proved that the Tb-HAp and HAp NWs membranes both presented high cytocompatibility and adequate efficiency to induce osteogenic differentiation of bone marrow stem cells (BMSCs). HAp/Tb-HAp CCS membranes were then implanted into a rat calvarial bone-defect model to study the behavior of HAp in bone repair process in vivo by tracking the fluorescence distribution. The results showed that the fluorescence of Tb-HAp diffused gradually from the inner circle to the outer ring, which suggested that the HAp was first degraded, and then the degraded product was diffused and finally reconstructed. Further, the histological results proved that the doping of Tb did not impair the promotive effect of HAp on bone repair process. Therefore, this study provided a visual method to observe the degradation-diffusion-reconstruction behavior of HAp nanomaterials in bone repair process. STATEMENT OF SIGNIFICANCE: The study of dynamic degradation process of implanted hydroxyapatite (HAp) materials in bone-defect cavity is of great significance to bone tissue engineering applications. Here, we designed a HAp/Tb-HAp nanowires (NWs) membrane with concentric circle structure (CCS) to visibly observe the behavior of HAp during bone repair process. HAp/Tb-HAp CCS membrane possessed both osteoinduction ability and fluorescence property. Calvarial bone-defect repair experiments in vivo showed that the fluorescence of Tb-HAp diffused gradually from inner circle to outer ring, which suggested that HAp was first degraded, then diffused and finally reconstructed. Therefore, this invention provides not only a visible method to observe the degradation-diffusion-reconstruction behavior of HAp-based biomaterials, but also a basic understanding of the dynamic change of HAp-based biomaterials.
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http://dx.doi.org/10.1016/j.actbio.2019.10.044DOI Listing
January 2020

Quaternary Piperazine-Substituted Rhodamines with Enhanced Brightness for Super-Resolution Imaging.

J Am Chem Soc 2019 09 9;141(37):14491-14495. Epub 2019 Sep 9.

State Key Laboratory of Fine Chemicals , Dalian University of Technology , Dalian 116024 , China.

Insufficient brightness of fluorophores poses a major bottleneck for the advancement of super-resolution microscopes. Despite being widely used, many rhodamine dyes exhibit sub-optimal brightness due to the formation of twisted intramolecular charge transfer (TICT) upon photoexcitation. Herein, we have developed a new class of quaternary piperazine-substituted rhodamines with outstanding quantum yields (Φ = 0.93) and superior brightness (ε × Φ = 8.1 × 10 L·mol·cm), by utilizing the electronic inductive effect to prevent TICT. We have also successfully deployed these rhodamines in the super-resolution imaging of the microtubules of fixed cells and of the cell membrane and lysosomes of live cells. Finally, we demonstrated that this strategy was generalizable to other families of fluorophores, resulting in substantially increased quantum yields.
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http://dx.doi.org/10.1021/jacs.9b04893DOI Listing
September 2019

Impact of Allergy and Eosinophils on the Morbidity of Chronic Rhinosinusitis with Nasal Polyps in Northwest China.

Int Arch Allergy Immunol 2019 10;179(3):209-214. Epub 2019 Apr 10.

Department of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, China.

Background: Nasal polyps are a common health problem that can significantly impact the quality of life.

Objective: To analyze the impact of allergy and peripheral eosinophils (EOS) on the morbidity of chronic rhinosinusitis with nasal polyps (CRSwNP) in Northwest China.

Methods: A retrospective cohort of 323 patients who underwent endoscopic sinus surgery (ESS) for chronic rhinosinusitis without nasal polyps (CRSsNP) and CRSwNP in Xijing Hospital was studied between January 5, 2011, and January 4, 2015. All of the patients underwent an allergen skin prick test and peripheral blood EOS inspection. Detailed information regarding the impact of allergy and EOS on the morbidity of CRSwNP was collected. Potential risk factors associated with nasal polyps were explored using logistic regression analysis. Multivariate logistic regression was performed to identify independent risk factors.

Results: The results revealed that EOS is an important risk factor for nasal polyps. In the univariate analysis, the adjusted OR was 2.01 (95% CI 1.08-3.72; p = 0.027). In the multivariate analysis, the adjusted OR was 2.02 (95% CI 1.08-3.76; p = 0.027). Compared to allergic rhinitis and normal EOS levels, nonallergic rhinitis and elevated EOS levels constituted a risk factor for CRSwNP (OR = 2.70; 95% CI 1.32-5.50). Compared to allergen-positive and EOS-normal status, allergen-negative and elevated-EOS status constituted a risk factor for CRSwNP (OR = 2.95; 95% CI 1.38-6.33).

Conclusion: EOS is a significant factor related to the morbidity of CRSwNP in Northwest China. Elevated EOS levels occurring in the context of nonallergic rhinitis constitute a risk factor for CRSwNP. Similarly, elevated EOS levels occurring in the context of allergen-negative rhinitis are also an important risk factor for morbidity of CRSwNP.
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http://dx.doi.org/10.1159/000497384DOI Listing
August 2019

Protective effect of different forms of nitrogen application on cadmium-induced toxicity in wheat seedlings.

Environ Sci Pollut Res Int 2019 May 20;26(13):13085-13094. Epub 2019 Mar 20.

College of Resource Science and Technology, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China.

Therefore, applying NH-N tp PHW-SA caused greater tolerance to Cd toxicity by higher biomass production, photosynthetic capacity, Ca and Cu accumulation, better root development and lower translocation factor of Cd as well as Cd concetration in organelle fraction. The Cd stress inhibited the growth performance of wheat seedlings, the mineral nutrient accumulation, and nitrogen uptake and distribution, and different forms of nitrogen have different protective effects on wheat. In PHW-SA, ANCd treatment caused lower reduction in biomass accumulation, photosynthesis, isotope stable N content, Ca and Cu accumulation, root development inhibition, tissue Cd concentration, and transfer factor, which even led to lower concentrations of Cd in F than those in F and F in comparison with NNCd treatment. On the other hand, the converse growth performance was recorded in J-11 under ANCd treatment. Meanwhile, the nitrogen absorption preference in PHW-SA was altered along with the enhanced absorption efficiency of nitrogen. Therefore, applying NH-N to PHW-SA caused greater tolerance to Cd toxicity by higher biomass production, photosynthetic capacity, Ca and Cu accumulation, better root development and lower translocation factor of Cd as well as Cd concetration in organelle fraction..
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http://dx.doi.org/10.1007/s11356-019-04747-5DOI Listing
May 2019

Development of Organic/Inorganic Compatible and Sustainably Bioactive Composites for Effective Bone Regeneration.

Biomacromolecules 2018 09 10;19(9):3637-3648. Epub 2018 Aug 10.

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry , Chinese Academy of Sciences , Changchun 130022 , P. R. China.

In this paper, we demonstrate a strategy of covalently bonding bioactive molecules onto inorganic hydroxyapatite (HAp) to improve the compatibility between organic and inorganic components and endow the bone composites with sustainable bioactivity. Bone morphogenetic protein-2 (BMP-2) peptide covalently immobilized nano-hydroxyapatite (nHAp-BMP-2) is developed to preserve the bioactivity and slow the release of the BMP-2 peptide. Then nHAp-BMP-2 was further incorporated into an ultraviolet-curable mixture of gelatin methacrylamide (GelMA) and four-armed PEG methacrylamide (four-armed PEGMA) to form a Gel/(nHAp-BMP-2) composite. The hydrogen bonding between gelatin and BMP-2 on nHAp-BMP-2 enhanced the compatibility between inorganic and organic components. The Gel/(nHAp-BMP-2) composite exhibited superior biocompatibility caused by gelatin and nHAp-BMP-2, except in a two-dimensional cell culture, the hydrogel was also capable of a three-dimensional cell culture. In addition, the introduction of nHAp-BMP-2 had a positive influence on bone marrow mesenchymal stem cell proliferation, differentiation, and the subsequent calcification on the composite. After treatment of a rat calvarial defect model for 12 weeks, the Gel/(nHAp-BMP-2) group showed the largest new bone volume and the highest ratio of new bone (50.54 ± 13.51 mm and 64.38 ± 17.22%, respectively) compared to those of the other groups. These results demonstrate that this way of controlling BMP-2 release is effective and the Gel/(nHAp-BMP-2) composite has great potential in bone regeneration therapy.
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http://dx.doi.org/10.1021/acs.biomac.8b00707DOI Listing
September 2018

Composite PLA/PEG/nHA/Dexamethasone Scaffold Prepared by 3D Printing for Bone Regeneration.

Macromol Biosci 2018 06 24;18(6):e1800068. Epub 2018 Apr 24.

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Street, Changchun, 130022, P. R. China.

3D printing has become an essential part of bone tissue engineering and attracts great attention for the fabrication of bioactive scaffolds. Combining this rapid manufacturing technique with chemical precipitation, biodegradable 3D scaffold composed of polymer matrix (polylactic acid and polyethylene glycol), ceramics (nano hydroxyapatite), and drugs (dexamethasone (Dex)) is prepared. Results of water contact angle, differential scanning calorimeter, and mechanical tests confirm that incorporation of Dex leads to significantly improved wettability, higher crystallinity degree, and tunable degradation rates. In vitro experiment with mouse MC3T3-E1 cells implies that Dex released from scaffolds is not beneficial for early cell proliferation, but it improves late alkaline phosphatase secretion and mineralization significantly. Anti-inflammation assay of murine RAW 264.7 cells proves that Dex released from all the scaffolds successfully suppresses lipopolysaccharide induced interleukin-6 and inducible nitric oxide synthase secretion by M1 macrophages. Further in vivo experiment on rat calvarial defects indicates that scaffolds containing Dex promote osteoinduction and osteogenic response and would be promising candidates for clinical applications.
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http://dx.doi.org/10.1002/mabi.201800068DOI Listing
June 2018

Enhanced removal of iodide ions by nano CuO/Cu modified activated carbon from simulated wastewater with improved countercurrent two-stage adsorption.

Sci Total Environ 2018 Jun 19;626:612-620. Epub 2018 Feb 19.

School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China.

A newly developed adsorbent nano CuO/Cu-modified activated carbon composite (nano CuO/Cu-C) was used to remove radioactive iodide ions (I) from simulated wastewater. The emphasis of this research is to improve adsorption performance and obtain higher I removal efficiency compared with the single-stage adsorption. To fully develop the amount of adsorption by nano CuO/Cu-C, and to increase the decontamination factor (DF) of I, an improved countercurrent two-stage adsorption (ICTA) process was introduced. In the ICTA process, measures dealing with desorption of loaded adsorbent in the stage-two adsorption were taken and more extensive application of countercurrent two-stage adsorption (CTA) process could be made after the improvement to ICTA process in this study. Furthermore, in order to analyze the process and determine the I concentration in the effluent, a calculation method was devised based on the Langmuir isotherm equations and adsorption accumulation principle. The mean DFs were 177, 166, and 89.7, respectively, when the initial I concentrations were 5.00, 10.0, and 20.0 mg/L; and the adsorbent dosage was 1.25 g/L. These results were approximately 8.76, 8.97, and 6.79 times higher, respectively, than with conventional single-stage adsorption. The experimental values of the I concentration were higher than the calculated ones, which could be ascribed to desorption of the residual loaded adsorbent and formation of CuI in the adsorption at stage 1. Formation of CuI in the adsorption at stage 1 was considered to be the predominant reason.
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http://dx.doi.org/10.1016/j.scitotenv.2018.01.078DOI Listing
June 2018

How Electroencephalogram Reference Influences the Movement Readiness Potential?

Front Neurosci 2017 11;11:683. Epub 2017 Dec 11.

Department of Automation, Tsinghua University, Beijing, China.

Readiness potential (RP) based on electroencephalograms (EEG) has been studied extensively in recent years, but no studies have investigated the influence of the reference electrode on RP. In order to investigate the reference effect, 10 subjects were recruited and the original vertex reference (Cz) was used to record the raw EEG signal when the subjects performed a motor preparation task. The EEG was then transformed to the common average reference (CAR) and reference electrode standardization technique (REST) reference, and we analyzed the RP waveform and voltage topographies and calculated the classification accuracy of idle and RP EEG segments. Our results showed that the RP waveform and voltage topographies were greatly influenced by the reference, but the classification accuracy was less affected if proper channels were selected as features. Since the Cz channel is near the primary motor cortex, where the source of RP is located, using the REST and CAR references is recommended to get accurate RP waveforms and voltage topographies.
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http://dx.doi.org/10.3389/fnins.2017.00683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732237PMC
December 2017

Molecular characterization, RNA interference and recombinant protein approach to study the function of the putative Molt Inhibiting Hormone (FmMIH1) gene from the shrimp Fenneropenaeus merguiensis.

Peptides 2019 12 14;122:169854. Epub 2017 Dec 14.

Fisheries College, Guangdong Ocean University, Zhanjiang, PR China. Electronic address:

The Molt Inhibiting Hormone gene and cDNA of the banana shrimp Fenneropenaeus merguiensis (FmMIH1) has been cloned and characterized. FmMIH1 possesses most of the characteristics of the eyestalk CHH/MIH/GIH family subtype-II neuropeptides. FmMIH1 open reading frame consists of 315 bp encoding for 105 amino acid residues. The mature peptide of FmMIH1 consists of 76 amino acid residues, a glycine residue at position 11 of the mature peptide and 6 cysteine residues located in the conserved position. In addition to eyestalk, high levels of FmMIH1 transcript could also be detected in the intestine. FmMIH1 transcript level is low throughout the post-molt, early to mid-intermolt and premolt. However, a sharp increase could be observed in late intermolt (C3 stage). Both alignment and phylogenetic analysis reveal that FmMIH1 is most similar to the MIH1 of other shrimps. For functional assay, RNA interference results show that a significant 2.3 days (P < 0.05) reduction in molt cycle duration could be observed in shrimp receiving dsFmMIH1 injection. Surprisingly, injection of recombinant FmMIH1 could also cause a significant reduction of the molt cycle (average 1.9 days, P < 0.05). We hypothesize that the recombinant protein is biological inactive but it competes with the endogenous MIH for carrier protein binding and consequently reduces the amount of biological MIH that could reach the targets. In conclusion, the result of this study will provide us new insight in molting/growth control in crustacean.
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http://dx.doi.org/10.1016/j.peptides.2017.10.017DOI Listing
December 2019