Publications by authors named "Xiaoyu Liu"

600 Publications

Different Responses of Soil Bacterial and Fungal Communities to 3 Years of Biochar Amendment in an Alkaline Soybean Soil.

Front Microbiol 2021 26;12:630418. Epub 2021 May 26.

Department of Bioengineering, College of Life Science, Huaibei Normal University, Huaibei, China.

Biochar as a soil amendment has been regarded as a promising way to improve soil fertility. However, the response of microbial community after biochar and biochar compound fertilizer (BCF) application has not been thoroughly elucidated. This study evaluated the changes in abundance and composition of bacterial and fungal communities using quantitative real-time PCR (qPCR) and Illumina MiSeq amplicon sequencing. The field experiment ran for 3 years and comprised five treatments: chemical fertilizer as control (CK), straw-returning combined with chemical fertilizer (CS), low biochar application combined with chemical fertilizer (LB), high biochar application combined with chemical fertilizer (HB) and BCF. The results showed that biochar amendment results no changes in the abundance and diversity of bacteria in the bulk and rhizosphere soils. However, the abundance of soil fungi was significantly increased by biochar amendment (LB and HB). LB treatment significantly increased the fungal alpha diversity, while there was no significant change under HB. Furthermore, the dominant bacterial phyla found in the samples were , , and . Biochar addition increased the relative abundance of in both bulk and rhizosphere soils. The dominant fungal phyla were , , and . The relative abundance of significantly decreased, but significantly increased in LB and HB. In addition, redundancy analysis indicated that the changes in bacterial and fungal communities are associated with soil properties such as SOC and TN, which are crucial contributors in regulating the community composition. This study is expected to provide significant theoretical and practical knowledge for the application of biochar in agricultural ecosystem.
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http://dx.doi.org/10.3389/fmicb.2021.630418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187762PMC
May 2021

Liquid-Phase Exfoliated Few-Layer Iodine Nanosheets for High-Rate Lithium-Iodine Batteries.

Chempluschem 2021 Jun;86(6):865-869

College of Applied Science and Technology, Beijing Union University, Beijing, 100012, P. R. China.

Rechargeable lithium-iodine (Li-iodine) batteries attract significant attention owning to their high energy density, wide abundance and low cost of iodine resources. However, iodine suffers from low electrical conductivity and high solubility in aprotic electrolyte, leading to fast capacity degradation, low columbic efficiency, as well as poor rate capability. Herein, we propose a simple method for the large-scale production of two-dimensional (2D) few-layer iodine nanosheets (FLINs) via liquid-phase exfoliation of iodine in deionized water. 2D FLINs could effectively improve rate capability by providing sufficient active sites and shortening the Li ion diffusion path. Meanwhile, graphene oxide (GO)@carbon nanotubes (CNT) hosts are designed to suppress the dissolution of iodine and enhance the electrical conductivity. [email protected]@FLINs film exhibits excellent rate capability (220 mAh g at 0.2 A g and 96 mAh g at 8 A g ) and outstanding cycle stability (93.2 mAh g at 2 A g after 1000 cycles) for lithium storage due to the synergistic effects of [email protected] hosts and 2D structure of FLINs. The controllable synthesis of FLINs provides a bright prospect for achieving high rate capability of Li-iodine batteries and is of utmost importance to potential large-scale applications.
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http://dx.doi.org/10.1002/cplu.202100166DOI Listing
June 2021

Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study.

Cell Res 2021 Jun 9. Epub 2021 Jun 9.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, China.

Emerging evidence suggests that children conceived through assisted reproductive technology (ART) have a higher risk of congenital heart defects (CHDs) even when there is no family history. De novo mutation (DNM) is a well-known cause of sporadic congenital diseases; however, whether ART procedures increase the number of germline DNM (gDNM) has not yet been well studied. Here, we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously. Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously, including 3.32 paternal and 1.26 maternal DNMs, after correcting for parental age at conception, cigarette smoking, alcohol drinking, and exercise behaviors. Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites, which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD. In addition, the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father. Among ART offspring, infertility of the father was associated with elevated paternal DNMs; usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs. In sum, the increased gDNMs in offspring conceived by ART were primarily originated from fathers, indicating that ART itself may not be a major reason for the accumulation of gDNMs. Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART.
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http://dx.doi.org/10.1038/s41422-021-00521-wDOI Listing
June 2021

CsPbX -ITO (X = Cl, Br, I) Nano-Heterojunctions: Voltage Tuned Positive to Negative Photoresponse.

Small 2021 Jun 9:e2101403. Epub 2021 Jun 9.

Key Lab of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University Beijing, Beijing, 100084, P. R. China.

All-Inorganic perovskite CsPbX (X = Cl, Br, I) quantum dots (QDs) have attracted tremendous attention in the past few years for their appealing performance in optoelectronic applications. Major properties of CsPbX QDs include the positive photoconductivity (PPC) and the defect tolerance of the in-band trap states. Here it is reported that when hybridizing CsPbX QDs with indium tin oxide (ITO) nanocrystals to form CsPbX -ITO nano-heterojunctions (NHJs), a voltage tuned photoresponse-from PPC to negative photoconductivity (NPC) transform-is achieved in lateral drain-source structured ITO/CsPbX -ITO-NHJs/ITO devices. A model combining exciton, charge separation, transport, and most critical the voltage driven electron filling of the in-band trap states with drain-source voltage (V ) above a threshold, is proposed to understand this unusual PPC-NPC transform mechanism, which is different from that of any known nanomaterial system. This finding exhibits potentials for developing devices such as photodetectors, optoelectronic switches, and memories.
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http://dx.doi.org/10.1002/smll.202101403DOI Listing
June 2021

Transformation of antiviral ribavirin during ozone/PMS intensified disinfection amid COVID-19 pandemic.

Sci Total Environ 2021 May 27;790:148030. Epub 2021 May 27.

State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Ministry of Education Key Laboratory of Yangtze River Water Environment, Tongji University, Shanghai 200092, China; International Joint Research Center for Sustainable Urban Water System, Tongji University, Shanghai 200092, China. Electronic address:

Due to the spread of coronavirus disease 2019 (COVID-19), large amounts of antivirals were consumed and released into wastewater, posing risks to the ecosystem and human health. Ozonation is commonly utilized as pre-oxidation process to enhance the disinfection of hospital wastewater during COVID-19 spread. In this study, the transformation of ribavirin, antiviral for COVID-19, during ozone/PMS‑chlorine intensified disinfection process was investigated. •OH followed by O accounted for the dominant ribavirin degradation in most conditions due to higher reaction rate constant between ribavirin and •OH vs. SO• (1.9 × 10 vs. 7.9 × 10 M s, respectively). During the O/PMS process, ribavirin was dehydrogenated at the hydroxyl groups first, then lost the amide or the methanol group. Chloride at low concentrations (e.g., 0.5- 2 mg/L) slightly accelerated ribavirin degradation, while bromide, iodide, bicarbonate, and dissolved organic matter all reduced the degradation efficiency. In the presence of bromide, O/PMS process resulted in the formation of organic brominated oxidation by-products (OBPs), the concentration of which increased with increasing bromide dosage. However, the formation of halogenated OBPs was negligible when chloride or iodide existed. Compared to the O/HO process, the concentration of brominated OBPs was significantly higher after ozonation or the O/PMS process. This study suggests that the potential risks of the organic brominated OBPs should be taken into consideration when ozonation and ozone-based processes are used to enhance disinfection in the presence of bromide amid COVID-19 pandemic.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154182PMC
May 2021

MicroRNA-186-3p attenuates tumorigenesis of cervical cancer by targeting MCM2.

Oncol Lett 2021 Jul 19;22(1):539. Epub 2021 May 19.

Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei 075000, P.R. China.

The present study examined the effect of microRNA (miRNA/miR)-186-3p and its target gene, minichromosome maintenance complex component 2 (MCM2), on cervical cancer. Cervical cancer tissues and corresponding normal tissues were collected from 48 patients and bioinformatics analysis was performed to identify the differentially expressed genes in cervical cancer. TargetScan and TarBase were used to identify miRNAs, and reverse transcription-quantitative PCR was conducted to detect and evaluate mRNA expression levels. Additionally, MTT and 5-bromo-2-deoxyuridine assays were performed to examine cell proliferation. Cell adhesion, cell cycle distribution and apoptosis were assessed using cell adhesion, flow cytometry and caspase-3/7 activity assays, respectively. The results revealed that miR-186-3p expression was downregulated in cervical cancer tissues and cells, and it negatively regulated MCM2 expression by directly targeting its 3' untranslated region in cervical cancer. Furthermore, MCM2 facilitated cell proliferation and inhibited cell apoptosis, which were reversed by upregulation of miR-186-3p expression. Collectively, the present study suggested that MCM2 and its negative regulator, miR-186-3p, regulate cervical cancer progression.
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http://dx.doi.org/10.3892/ol.2021.12800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161468PMC
July 2021

Chromosome-scale assembly of the Sparassis latifolia genome obtained using long-read and Hi-C sequencing.

G3 (Bethesda) 2021 May 21. Epub 2021 May 21.

Institute of Edible Mushroom, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China; National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Mushroom, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China.

Sparassis latifolia is a valuable edible mushroom cultivated in China. In 2018, our research group reported an incomplete and low-quality genome of S. latifolia obtained by Illumina HiSeq 2500 sequencing. These limitations in the available genome have constrained genetic and genomic studies in this mushroom resource. Herein, an updated draft genome sequence of S. latifolia was generated by Oxford Nanopore sequencing and the Hi-C technique. A total of 8.24 Gb of Oxford Nanopore long reads representing ∼198.08X coverage of the S. latifolia genome were generated. Subsequently, a high-quality genome of 41.41 Mb, with scaffold and contig N50 sizes of 3.31 Mb and 1.51 Mb, respectively, was assembled. Hi-C scaffolding of the genome resulted in 12 pseudochromosomes containing 93.56% of the bases in the assembled genome. Genome annotation further revealed that 17.47% of the genome was composed of repetitive sequences. In addition, 13,103 protein-coding genes were predicted, among which 98.72% were functionally annotated. BUSCO assay results further revealed that there were 92.07% complete BUSCOs. The improved chromosome-scale assembly and genome features described here will aid further molecular elucidation of various traits, breeding of S. latifolia, and evolutionary studies with related taxa.
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http://dx.doi.org/10.1093/g3journal/jkab173DOI Listing
May 2021

Dual Resistance and Impedance Investigation: Ultrasensitive and Stable Humidity Detection of Molybdenum Disulfide Nanosheet-Polyethylene Oxide Hybrids.

ACS Appl Mater Interfaces 2021 Jun 20;13(21):25250-25259. Epub 2021 May 20.

Key Laboratory of Optoelectronic Technology and System of Ministry of Education, College of Optoelectronic Engineering, Chongqing University, Chongqing 400044, China.

There is an imperative demand for real-time relative humidity (RH) discrimination with excellent sensitivity and robust operation stability over a broad RH range at room temperature (22 °C). Of diverse two-dimensional (2D) materials, -type molybdenum disulfide (MoS) as a typical gas-sensing candidate has been rarely harnessed for humidity detection due to tiny response and undesirable stability induced by the conversion from electron to proton conduction with increasing RH. To overcome these issues, MoS-polyethylene oxide (PEO) inorganic-organic nanocomposites as the sensing layer were facilely prepared in this work. The results showed that the composition-optimized composite film sensor surpassed the isolated MoS counterpart in terms of repeatability, response, hysteresis, stability, and selectivity. Both DC-resistance and AC-impedance analyses unveiled different roles of MoS and PEO components within composites. MoS strengthened the film structure, while hydrophilic PEO enlarged the water-adsorption capacity and thus improved the response and detection reliability via water-triggered ionic conductivity. This work afforded a feasible strategy via inorganic-organic combination to distinguish trace RH and improved the operation stability of 2D material-based sensors, simultaneously demonstrating realistic monitoring applications of exhaled gas detection and distance variation of moisture-emitting objects.
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http://dx.doi.org/10.1021/acsami.1c02119DOI Listing
June 2021

Anti-apoptotic Effect of MiR-223-3p Suppressing PIK3C2A in Cardiomyocytes from Myocardial Infarction Rat Through Regulating PI3K/Akt Signaling Pathway.

Cardiovasc Toxicol 2021 Aug 17;21(8):669-682. Epub 2021 May 17.

The Third Department of Cardiology, Cangzhou Central Hospital, No.16 Xinhua West Road, Yunhe District, Cangzhou City, 061000, People's Republic of China.

We aimed to explore the regulatory mechanism of the axis of miR-223-3p-PIK3C2A-PI3K/Akt on cardiomyocyte apoptosis in rats with myocardial infarction. Thirty 8-week-old healthy male SD rats were used for establishing the sham group and the model group, with HE staining, TUNEL staining, and TTC staining performed. After the identification of the targeting relationship between PIK3C2A and miR-223-3p, experimental rats were randomly divided into seven groups by plasmid transfection, including the Blank group, negative control (NC) group, miR-223-3p mimic group, miR-223-3p inhibitor group, siRNA-PIK3C2A group, oe-PIK3C2A group, and miR-223-3p inhibitor + oe-PIK3C2A group. Four weeks after transfection, the expression levels of miR-223-3p and PIK3C2A in tissues as well as PI3K, Akt, Bax, and bcl-2 mRNA in cells were detected by qRT-PCR and western blot, in combination with the detection of apoptosis rate by flow cytometry. Compared with the sham group, the model group showed typical myocardial injury and abnormal staining, higher apoptotic index, and larger myocardial infarction area (all P < 0.05). PIK3C2A was the target gene of miR-223-3p. The expression level of miR-223-3p in model group was significantly lower than that in sham group, while the mRNA and protein expression levels of PIK3C2A increased significantly (all P < 0.05). In cell tests, the expression level of miR-223-3p increased significantly in miR-223-3p mimic group (P < 0.05), which, however, showed no significant change in siRNA-PIK3C2A group (P > 0.05). MiR-223-3p inhibitor group and siRNA-PIK3C2A group had obviously increased PI3K, Akt, mTOR and Bcl-2 mRNA, and protein expression, while decreased mRNA and protein expression of PIK3C2A and Bax (all P < 0.05); miR-223-3p mimic groups had the opposite trends (all P < 0.05). siRNA-PIK3C2A + miR-223-3p mimic showed no obvious change relative to the control groups (all P > 0.05). Low expression of miR-223-3p may downregulate PIK3C2A expression, resulting in the inhibition of myocardial cell apoptosis in rats with myocardial infarction via the activation of PI3K/Akt signaling pathway.
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http://dx.doi.org/10.1007/s12012-021-09658-xDOI Listing
August 2021

Factors predicting the recovery from acute kidney injury in children with primary nephrotic syndrome.

Clin Exp Nephrol 2021 May 15. Epub 2021 May 15.

Department of Pediatrics, Peking University First Hospital, No.1 Xi An Men Da Jie, Beijing, China.

Background: The prognosis of acute kidney injury (AKI) varies in children with nephrotic syndrome (NS), data on factors predicting the recovery and recurrence of AKI in children with NS are limited. This study aimed to explore the possible factors predicting the recovery from and recurrence of AKI in children with primary NS.

Methods: Children with primary NS complicated with AKI from 1993 to 2017 in a single centre were reviewed retrospectively. The clinical pictures and possible factors predicting the recovery from and recurrence of AKI in children with primary NS were investigated.

Results: Sixty-eight episodes of AKI in 59 children with NS were analysed: 88.2% of AKI recovered within 3 months, and 2.9% of AKI did not recover after 3 months. Survival analysis revealed that leucocyturia is significantly related to the AKI recovery time (P = 0.001), and children with leucocyturia [22 (4, 79) days] recovered significantly slower than did children without leucocyturia [12.0 (2, 39) days]. Renal tubular and interstitial injury were prominent in children with leucocyturia, and 11.9% of children with index AKI experienced the recurrence of AKI.

Conclusions: Most episodes of AKI that occurred in children with NS recovered completely. Leucocyturia is a significant factor predicting the recovery time of AKI.
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http://dx.doi.org/10.1007/s10157-021-02074-zDOI Listing
May 2021

Aquaporin 4 differentially modulates osmotic effects on vasopressin neurons in rat supraoptic nucleus.

Acta Physiol (Oxf) 2021 May 12:e13672. Epub 2021 May 12.

Department of Physiology, Harbin Medical University, Harbin, 150086, China.

Aim: Glial fibrillary acidic protein (GFAP) has molecular association with aquaporin 4 (AQP4) in astrocytic plasticity. Here, we further examined how AQP4 modulates osmotic effects on vasopressin (VP) neurons in rat supraoptic nucleus (SON) through interactions with GFAP.

Methods: Brain slices from adult male rats were under osmotic stimulation. Western blot, co-immunoprecipitation, immunohistochemistry and patch-clamp recordings were used for analyzing expressions and interactions between GFAP and AQP4 as well as their influence on VP neuronal activity. Data were analyzed using SPSS software.

Results: Hyposmotic challenge (HOC) of acute SON slices did not increase global AQP4 level, but caused a transient increase in the colocalization of AQP4 with GFAP filaments within 5 min. This effect was prominent at astrocytic processes surrounding VP neuron somata and accompanied with inhibition of VP neuronal activity. Similar HOC effect was seen in the SON that underwent in vivo HOC wherein transiently increased molecular association between GFAP and AQP4 was detected using co-immunoprecipitation. The rebound excitation at late stage (10 min) of VP neurons following HOC in brain slices and the associated GFAP's "returning to normal" around VP neurons were both hampered by 2-(nicotinamide)-1,3,4-thiadiazole, a specific AQP4 channel blocker that itself did not influence VP neuronal activity. Moreover, this agent prevented hyperosmotic stress-evoked excitation of VP neurons and associated reduction of GFAP filaments.

Conclusion: These findings indicate that osmotically-driven increase in VP neuronal activity requires the activation of AQP4, which determines a retraction of GFAP filaments.
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http://dx.doi.org/10.1111/apha.13672DOI Listing
May 2021

Spermidine inhibits vascular calcification in chronic kidney disease through modulation of SIRT1 signaling pathway.

Aging Cell 2021 Jun 9;20(6):e13377. Epub 2021 May 9.

Department of Cardiology, Laboratory of Heart Center, Heart Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Vascular calcification is a common pathologic condition in patients with chronic kidney disease (CKD) and aging individuals. It has been established that vascular calcification is a gene-regulated biological process resembling osteogenesis involving osteogenic differentiation. However, there is no efficient treatment available for vascular calcification so far. The natural polyamine spermidine has been demonstrated to increase life span and protect against cardiovascular disease. It is unclear whether spermidine supplementation inhibits vascular calcification in CKD. Alizarin red staining and quantification of calcium content showed that spermidine treatment markedly reduced mineral deposition in both rat and human vascular smooth muscle cells (VSMCs) under osteogenic conditions. Additionally, western blot analysis revealed that spermidine treatment inhibited osteogenic differentiation of rat and human VSMCs. Moreover, spermidine treatment remarkably attenuated calcification of rat and human arterial rings ex vivo and aortic calcification in rats with CKD. Furthermore, treatment with spermidine induced the upregulation of Sirtuin 1 (SIRT1) in VSMCs and resulted in the downregulation of endoplasmic reticulum (ER) stress signaling components, such as activating transcription factor 4 (ATF4) and CCAAT/enhancer-binding protein homologous protein (CHOP). Both pharmacological inhibition of SIRT1 by SIRT1 inhibitor EX527 and knockdown of SIRT1 by siRNA markedly blocked the inhibitory effect of spermidine on VSMC calcification. Consistently, EX527 abrogated the inhibitory effect of spermidine on aortic calcification in CKD rats. We for the first time demonstrate that spermidine alleviates vascular calcification in CKD by upregulating SIRT1 and inhibiting ER stress, and this may develop a promising therapeutic treatment to ameliorate vascular calcification in CKD.
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http://dx.doi.org/10.1111/acel.13377DOI Listing
June 2021

Feasibility of Methylated in Stool for Early Detection of Colorectal Cancer: A Training Study in Chinese Population.

Front Oncol 2021 22;11:647066. Epub 2021 Apr 22.

Department of Gastroenterology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Background: Early detection of colorectal cancer (CRC) and precancerous lesion is vitally important for mitigating CRC morbidity and mortality. Aberrant DNA methylations in certain promoter regions have been identified to be closely associated with CRC development and progression, suggesting their potential as diagnostic biomarkers for early detection. In this study, we evaluated the performance of methylated in stool specimens as a potential biomarker for CRC detection.

Methods: A total of 321 subjects out of 365 enrolled participants were included in the final analysis, including 154 CRC patients, 23 advanced adenoma (AA) patients, 49 small polyp (SP) patients, and 95 healthy controls. methylation level was examined by qPCR with bisulfite converted DNA purified from approximately 5 g stool specimen.

Results: Methylated test showed high sensitivities of 78.3% (95% CI: 55.8%-91.7%) and 90.3% (95% CI: 84.2%-94.3%) for detecting AA and CRC, respectively, with a specificity of 88.4% (95% CI: 79.8%-93.8%). methylation level discriminated AA and CRC patients from control subjects with area under the curve values of 0.892 (95% CI: 0.795-0.988) and 0.961 (95% CI: 0.938-0.983). Further analysis indicated no significant difference in sensitivities among different ages, genders, stages, locations, sides, tumor sizes and differentiation statuses.

Conclusions: Methylated showed a strong potential as a noninvasive biomarker for early CRC detection.
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http://dx.doi.org/10.3389/fonc.2021.647066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100593PMC
April 2021

Could biochar amendment be a tool to improve soil availability and plant uptake of phosphorus? A meta-analysis of published experiments.

Environ Sci Pollut Res Int 2021 May 8. Epub 2021 May 8.

Institute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, Jiangsu, China.

As one of the most important nutrients for plant growth, phosphorus was often poorly available in soil. While biochar addition induced improvement of soil structure, nutrient and water retention as well as microbial activity had been well known, and the effect of biochar soil amendment (BSA) on soil phosphorus availability and plant P uptake had been not yet quantitatively assessed. In a review study, data were retrieved from 354 peer-reviewed research articles on soil available P content and P uptake under BSA published by February 2019. Then a database was established of 516 data pairs from 86 studies with and without BSA in agricultural soils. Subsequently, the effect size of biochar application was quantified relative to no application and assessed in terms of biochar conditions, soil conditions, as well as experiment conditions. In grand mean, there was a significant and great effect of BSA on soil available P and plant P uptake by 65% and 55%, respectively. The effects were generally significant under manure biochar, biochar pyrolyzed under 300 °C, soil pH <5 and fine-textured soil, and soils that are very low in available P. Being significantly correlated to soil P availability (R=0.29), plant P uptake was mostly enhanced with vegetable crops of high biomass yield. Overall, biochar amendment at a dosage up to 10 t ha could be a tool to enhance soil availability and plant uptake of phosphorus, particularly in acid, heavy textured P-poor soils.
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http://dx.doi.org/10.1007/s11356-021-14119-7DOI Listing
May 2021

Depression and anxiety symptoms are related to pain and frailty but not cognition or delirium in older surgical patients.

Brain Behav 2021 May 5:e02164. Epub 2021 May 5.

Department of Anesthesiology, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA.

Objective: In community dwelling older adults, depression and anxiety symptoms can be associated with early cognitive decline. Symptoms of depression and anxiety are common in older adults prior to surgery. However, their significance is unknown. Our objective was to determine whether preoperative depression and anxiety symptoms are associated with postoperative cognitive decline (POCD) and in-hospital delirium, in older surgical patients.

Methods: We conducted a secondary data analysis of postoperative cognitive dysfunction in a cohort study of patients 65 and older undergoing elective noncardiac surgery. We used the Hospital Anxiety and Depression Scale (HADS) to screen for depression and anxiety symptoms at a home visit prior to surgery and 3 months after surgery. Patients with a history of psychiatric (major depressive disorder, bipolar disorder, and schizophrenia) or neurologic disorder (Parkinson's disease and stroke) were excluded from the parent study.

Results: Out of the 167 patients, 9.6% (n = 16) reported significant depressive symptoms and 21.6% (n = 36) reported significant anxiety symptoms on preoperative screening. There was no association between preoperative or new-onset postoperative depression and anxiety symptoms and the incidence of delirium or POCD three months after surgery. Patients with preoperative depressive symptoms had higher preoperative pain (scores 69 vs. 35.7, p = .002) and frailty (56 vs. 14.6, p <.001).

Conclusion: In our cohort, we did not detect an association between preoperative depression and anxiety symptoms and neurocognitive disorders. Preoperative depression and anxiety symptoms were related to physical pain and frailty. Taken together, these suggest that in patients without a formal psychiatric diagnosis, preoperative depression and anxiety symptoms are related to physical state rather than a harbinger of early cognitive decline. Future studies are needed to understand the nature of the relationship between depression and anxiety symptoms and physical state in surgical patients.
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http://dx.doi.org/10.1002/brb3.2164DOI Listing
May 2021

The contributions of the left hippocampus and bilateral inferior parietal lobule to form-meaning associative learning.

Psychophysiology 2021 May 5:e13834. Epub 2021 May 5.

Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, Guangzhou, China.

Existing studies have identified crucial roles for the hippocampus and a distributed set of cortical regions (e.g., the inferior parietal cortex) in learning novel words. Nevertheless, researchers have not clearly determined how the hippocampus and cortical regions dynamically interact during novel word learning, especially during form-meaning associative learning. As a method to address this question, we used an online learning paradigm and representational similarity analysis to explore the contributions of the hippocampus and neocortex to form-meaning associative learning. Twenty-nine native Chinese college students were recruited to learn 30 form-meaning pairs, which were repeated 7 times during fMRI scan. Form-meaning associative learning elicited activations in a wide neural network including regions required for word processing (i.e., the bilateral inferior frontal gyrus and the occipitotemporal cortex), regions required for encoding (i.e., the bilateral parahippocampus and hippocampus), and regions required for cognitive control (i.e., the anterior cingulate cortex and dorsolateral prefrontal cortex). More importantly, our study revealed the differential roles of the left hippocampus and bilateral inferior parietal lobule (IPL) in form-meaning associative learning. Specifically, higher pattern similarity in the bilateral IPL in the early learning phase (repetitions 1 to 3) was related to better learning performance, while higher pattern similarity in the left hippocampus in the late learning phase (repetitions 5 to 7) was associated with better learning performance. These findings indicate that the hippocampus and cortical regions (e.g., the IPL) contribute to form-meaning learning in different stages.
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http://dx.doi.org/10.1111/psyp.13834DOI Listing
May 2021

Novel role of CAP1 in regulation RNA polymerase II-mediated transcription elongation depends on its actin-depolymerization activity in nucleoplasm.

Oncogene 2021 May 28;40(20):3492-3509. Epub 2021 Apr 28.

Department of Pharmacy, The Second Affiliated Hospital of Army Medical University, Chongqing, China.

Lung cancer is one of the most intractable diseases with high incidence and mortality worldwide. Adenylate cyclase-associated protein 1 (CAP1), a well-known actin depolymerization factor, is recently reported to be an oncogene accelerating cancer cell proliferation. However, the physiological significance of CAP1 in lung cancer is incompletely understood and the novel functions of CAP1 in transcriptional regulation remain unknown. Here we found that CAP1 was highly expressed in lung cancer tissues and cells, which was also negatively associated with prognosis in lung cancer patients. Moreover, CAP1 promoted A549 cells proliferation by promoting protein synthesis to accelerate cell cycle progression. Mechanistically, we revealed that CAP1 facilitated cyclin-dependent kinase 9 (CDK9)-mediated RNA polymerases (Pol) II-Ser2 phosphorylation and subsequent transcription elongation, and CAP1 performed its function in this progress depending on its actin-depolymerization activity in nucleoplasm. Furthermore, our in vivo findings confirmed that CAP1-promoted A549 xenograft tumor growth was associated with CDK9-mediated Pol II-Ser2 phosphorylation. Our study elucidates a novel role of CAP1 in modulating transcription by promoting polymerase II phosphorylation and suggests that CAP1 is a newly identified biomarker for lung cancer treatment and prognosis prediction.
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http://dx.doi.org/10.1038/s41388-021-01789-3DOI Listing
May 2021

The effects of cages implantation on surgical and adjacent segmental intervertebral foramina.

J Orthop Surg Res 2021 Apr 27;16(1):280. Epub 2021 Apr 27.

Department of Spine Surgery, China-Japan Union Hospital of Jilin University, 126 Xiantai St., Erdao District, Changchun City, People's Republic of China.

Objection: The overarching goal of our research was to compare the clinical and radiological outcomes with different sizes of cages implantation in anterior cervical discectomy and fusion (ACDF), and to evaluate the effects on surgical and adjacent segmental intervertebral foramina.

Methods: The clinical data of 61 patients were analyzed retrospectively. The radiological data included the surgical intervertebral disk space height before (H) and after surgery (H), the preoperative mean height of adjacent segments (H), the area and height of the surgical and adjacent segment foramen, the surgical segmental Cobb angle (α), and C2-7Cobb angle (α). The calculation of clinical data was conducted by Japanese Orthopaedic Association Scores (JOA), the recovery rate of JOA scores and visual analog scales (VAS). In accordance with the different ranges of distraction (H/H), patients were classified into three groups: group A (H/H<1.20, n=13), group B (1.20≤H/H≤1.80, n=37), and group C (H/H>1.80, n=11).

Results: After the operation and at the final follow-up, our data has demonstrated that the area and height of surgical segmental foramen all increased by comparing those of preoperation in three groups (all P<0.05). However, except for a decrease in group C (all P<0.05), the adjacent segmental foramina showed no significant changes (all P>0.05). The area and height of the surgical segment foramen and the distraction degree were positively correlated (0
Conclusion: The oversize cage might give a rise to a negative impact on the adjacent intervertebral foramen in ACDF. The mean value of the adjacent intervertebral disk space height (H) could be used as a reference standard. Moreover, the 1.20~1.80 fold of distraction (H/H) with optimal cages would achieve a better long-term prognosis.
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http://dx.doi.org/10.1186/s13018-021-02421-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077914PMC
April 2021

Total Synthesis of the Proposed Structure of Characellide B.

Org Lett 2021 05 27;23(9):3680-3684. Epub 2021 Apr 27.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of Active Substances Discovery and Druggability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

We achieved the total synthesis of the proposed structure of characellide B, a novel lipoglycotripeptide. Comparison of the data for the synthetic compound with those for the natural product indicated some possible errors in the original structural assignment. Furthermore, we synthesized the other four stereoisomers, focusing on the d-Asp-d--Thr fragment, to determine the actual structure of characellide B. Nevertheless, the data for the stereoisomers were not consistent with those for the natural product.
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http://dx.doi.org/10.1021/acs.orglett.1c01066DOI Listing
May 2021

Mindfulness-based interventions for social anxiety disorder: A systematic review and meta-analysis.

Psychiatry Res 2021 Jun 20;300:113935. Epub 2021 Apr 20.

Department of Medical Psychology, School of Mental Health and Psychological Science, Anhui Medical University, Hefei, China. Electronic address:

Various psychiatric disorders are treated with mindfulness-based interventions (MBIs), although the efficacy of MBIs in patients with social anxiety disorder (SAD) is unclear. In this meta-analysis, we investigated the efficacy of MBIs on SAD symptoms. Systematic searches were performed in various databases, and 11 eligible randomized controlled trials (RCTs) and 5 single-arm trials were identified. The between-groups analysis of the 11 RCTs showed that Hedges' g = 0.00, while the within-group analysis showed a large pre-post effect size (g = 1.20).MBIs were superior to the no-treatment comparator (g = 0.89), equivalent to specific active treatment (g = -0.19), and less effective than evidence-based treatment (i.e., cognitive behavioral therapies) (g = -0.29).MBIs significantly alleviated depressive symptoms and improved mindfulness, quality of life, and self-compassion. Meta-regression analysis showed a dose-response relationship between the alleviation of SAD symptoms and the duration of the MBIs (β = 0.659). Follow-up analysis showed that the effects of MBIs on SAD persisted for 12 months (g = 0.231). An analysis of the 5 single-arm trials found that MBIs had a medium effect in alleviating SAD symptoms (g = 0.48). Future research is needed regarding the design of large RCTs of MBIs on SAD patients.
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http://dx.doi.org/10.1016/j.psychres.2021.113935DOI Listing
June 2021

Similar activation patterns in the bilateral dorsal inferior frontal gyrus for monolingual and bilingual contexts in second language production.

Neuropsychologia 2021 Jun 16;156:107857. Epub 2021 Apr 16.

Key Laboratory of Brain, Cognition and Education Sciences (South China Normal University), Ministry of Education, China; School of Psychology, South China Normal University, 510631, Guangzhou, China; Center for Studies of Psychological Application, South China Normal University, 510631, Guangzhou, China; Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, 510631, Guangzhou, China. Electronic address:

Language production is a vital process of communication. Although many studies have devoted to the neural mechanisms of language production in bilinguals, they mainly focused on the mechanisms of cognitive control during language switching. Therefore, it is not clear how naming context influences the neural representations of linguistic information during language production in bilinguals. To address that question, the present study adopted representational similarity analysis (RSA) to investigate the neural pattern similarity (PS) between the monolingual and bilingual contexts separately for native and second languages. Consistent with previous findings, bilinguals behaviorally performed worse, and showed greater activation in brain regions for cognitive control including the anterior cingulate cortex and dorsolateral prefrontal cortex in the bilingual context relative to the monolingual context. More importantly, RSA revealed that bilinguals exhibited similar neural activation patterns in the bilateral dorsal inferior frontal gyrus between the monolingual and bilingual contexts in the production of the second language. Moreover, higher cross-context PS in the right inferior frontal gyrus was associated with smaller differences in naming speed of second language between the monolingual and bilingual contexts. These results suggest that similar linguistic representations are encoded for the monolingual and bilingual contexts in the production of non-dominant language.
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http://dx.doi.org/10.1016/j.neuropsychologia.2021.107857DOI Listing
June 2021

LINC01207 is up-regulated in gastric cancer tissues and promotes disease progression by regulating miR-671-5p/DDX5 axis.

J Biochem 2021 Apr 15. Epub 2021 Apr 15.

Department of Gastroenterology, Yantai Municipal Laiyang Central Hospital, Yantai 265200, Shandong Province, China.

LINC01207 is involved in the progression of some cancers. This study was designed to delve into the biological function and mechanism of LINC01207 in gastric cancer. qPCR was adopted to examine the expression levels of LINC01207, miR-671-5p, DDX5 mRNA in gastric cancer tissues and cells. After LINC01207 was overexpressed or depleted, MTT and BrdU assays were conducted to detect cell proliferation. Transwell assay was employed to detect cell migration and invasion. Western blot was used to detect the expression of DDX5 protein in cells. Bioinformatics analysis, luciferase reporter assay and RNA pull-down assay were performed to predict and validate the binding site between miR-671-5p and LINC01207 or DDX5. LINC01207, DDX5 mRNA were up-regulated in gastric cancer, while miR-671-5p was down-regulated; high expression of LINC01207 and transfection of miR-671-5p inhibitors facilitated the proliferation of gastric cancer cells; however, knocking down LINC01207 and the overexpression of miR-671-5p mimics had opposite biological effects. LINC01207 and miR-671-5p were interacted and miR-671-5p was negatively regulated by LINC01207. MiR-671-5p could reverse the function of LINC01207. DDX5 was a downstream target of miR-671-5p and was positively modulated by LINC01207. LINC01207 promotes the proliferation and metastasis of gastric cancer cells by regulating miR-671-5p/DDX5 axis.
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http://dx.doi.org/10.1093/jb/mvab050DOI Listing
April 2021

Methylated and in stool specimens for Colorectal Cancer early detection: A cost-effective strategy for Chinese population.

J Cancer 2021 5;12(9):2665-2672. Epub 2021 Mar 5.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210009, China.

The aim of this study was to evaluate the feasibility of combination of methylated and methylated (SpecColon test) in stool specimens for colorectal cancer (CRC) early detection and to optimize the cut-off value of methylated and methylated . Approximately 5 g of stool specimen each was collected from 420 subjects (291 in the training cohort and 129 in the validation cohort). Stool DNA was extracted and bisulfite-converted, followed by detection of methylated level of and . Youden index was employed to determine the cut-off value. The whole operating time for stool SpecColon test takes less than 5 hours. The limit of detection of combination of methylated and methylated was as low as 5 pg per reaction. The optimized cut-off value was methylated analyzed by 3/3 rule and methylated analyzed by 2/3 rule. In the training cohort, the sensitivities of stool SpecColon test for detecting AA and early stage CRC (stage 0-II) were 53.8% (95% CI: 26.1%-79.6%) and 89.1% (95% CI: 77.1%-95.5%) with a specificity of 93.5% (95% CI: 87.2%-96.9%), and the AUC for CRC diagnosis was 0.879 (95% CI: 0.830-0.928). Similar performance was achieved by SpecColon test also in the validation cohort, where its sensitivities for detecting AA and early stage CRC (stage 0-II) were 61.5% (95% CI: 32.3-84.9%) and 88.5% (95% CI: 68.5%-97.0%) with a specificity of 89.5% (95% CI: 74.3-96.7%). Combined detections of methylated and methylated in stool samples demonstrated high sensitivities and specificity for the detection of AA and early stage CRC. Therefore, this combination has the potential to become an accurate and cost-effective tool for CRC early detection.
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http://dx.doi.org/10.7150/jca.52478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040708PMC
March 2021

Acetylation of NDUFV1 induced by a newly synthesized HDAC6 inhibitor HGC rescues dopaminergic neuron loss in Parkinson models.

iScience 2021 Apr 17;24(4):102302. Epub 2021 Mar 17.

Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, China.

It has been shown that histone deacetylase (HDAC) inhibitors hold considerable therapeutic potentials for treating neurodegeneration-related diseases including Parkinson disease (PD). Here, we synthesized an HDAC inhibitor named as HGC and examined its neuroprotective roles in PD models. Our results showed that HGC protects dopaminergic neurons from 1-methyl-4-phenylpyridinium (MPP)-induced insults. Furthermore, in 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced PD model mice, HGC application rectifies behavioral defects, improves tyrosine hydroxylase-positive neurons in the midbrain, and maintains mitochondrial integrity and functions. Mechanistically, mass spectrometry data revealed that HGC stimulates acetylation modification at lysine 28 of NDUFV1. Inhibition of HDAC6 by HGC is responsible for this acetylation modification. Functional tests showed that, as well as HGC, NDUFV1 exhibits beneficial roles against MPP injuries. Moreover, knockdown of NDUFV1 abolishes the neuroprotective roles of HGC. Taken together, our data indicate that HGC has a great therapeutic potential for treating PD and NDUFV1 might be a target for developing drugs against PD.
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http://dx.doi.org/10.1016/j.isci.2021.102302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022854PMC
April 2021

Author Correction: Hydrochar did not reduce rice paddy NH volatilization compared to pyrochar in a soil column experiment.

Sci Rep 2021 Apr 13;11(1):8479. Epub 2021 Apr 13.

Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

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http://dx.doi.org/10.1038/s41598-021-88214-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044139PMC
April 2021

Sorption and migration of organophosphate flame retardants between sources and settled dust.

Chemosphere 2021 Sep 29;278:130415. Epub 2021 Mar 29.

Jacobs, Critical Mission Solutions, EPA - Research Laboratory Support, Research Triangle Park, NC, 27711, USA.

Dust serves as a strong sink for indoor pollutants, such as organophosphorus flame retardants (OPFRs). OPFRs are semivolatile chemicals that are slow in emissions but have long-term effects in indoor environments. This research studied the emission, sorption, and migration of OPFRs tris(2-chloroethyl) phosphate, tris(1-chloro-2-propyl) phosphate, and tris(1,3-dichloro-2-propyl) phosphate, from different sources to settled dust on OPFR source surfaces and OPFR-free surfaces. Four sink effect tests and six dust-source migration tests, including direct contact and sorption tests were conducted in 53 L stainless steel small chambers at 23 °C and 50% relative humidity. OPFR emission concentrations, and sorption and migration rates were determined. The dust-air and dust-material partition coefficients were estimated based on the experimental data and compared with those from the literature obtained by empirical equations. They are in the range of 1.4 × 10 to 2.6 × 10 (dimensionless) for the dust-air equilibrium partition coefficients and 2.38 × 10 to 0.8 (dimensionless) for the dust-material equilibrium partition coefficients. It was observed that the dust with less organic content and smaller size tended to absorb more OPFRs, but different dust did not significantly affect OPFRs emission from the same source to the chamber air. The dust-air partition favored the less volatile OPFRs in the house dust, whereas the emission from the source favored the volatile chemicals. Volatility of the chemicals had much less effect on dust-source partitioning than on dust-air partitioning. The results from this work improve our understating of the fate and mass transfer mechanisms between OPFRs sources, indoor air, surface, and dust.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204724PMC
September 2021

MiR-20a-5p overexpression prevented diabetic cardiomyopathy via inhibition of cardiomyocyte apoptosis, hypertrophy, fibrosis, and JNK/NF-κB signaling pathway.

J Biochem 2021 Apr 9. Epub 2021 Apr 9.

Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, China.

Diabetic cardiomyopathy (DCM) is a common cardiovascular disease. A declined miR-20a-5p was observed in hearts of diabetic mice, while its effect on DCM remains unknown. Herein, we established streptozotocin-induced DCM rat model and high glucose-stimulated H9C2 model of DCM. They then were treated with adenovirus expressing miR-20a-5p to explore the function of miR-20a-5p. ITT and ipGTT assay revealed that miR-20a-5p reduced blood glucose level. Besides, miR-20a-5p improved cardiac dysfunction reflected by reduced HW/BW and LVDP, and increased LVSP and ±LV dp/dt max. MiR-20a-5p prevented cardiomyocyte apoptosis, along with the up-regulated c-caspase-3, bax and down-regulated bcl-2. Moreover, miR-20a-5p alleviated cardiac hypertrophy as the parameters of ANP, BNP and MyHC-β decreased. Also, miR-20a-5p attenuated the cardiac fibrosis demonstrated by decreased TGF-β1, collagen I levels and the inflammatory response manifested by reduced IL-6, TNF-α and IL-1β production. Furthermore, miR-20a-5p prevented JNK phosphorylation and NF-κB p65nuclear translocation. Similarly, the effects of miR-20a-5p on DCM were confirmed in our in vitro experiments. Additionally, ROCK2 is a possible target gene of miR-20a-5p. ROCK2 overexpression reversed the protective effect of miR-20a-5p on DCM. Overall, miR-20a-5p may effectively ameliorate DCM through improving cardiac metabolism, and subsequently inhibiting inflammation, apoptosis, hypertrophy, fibrosis, and JNK/NF-κB pathway via modulating ROCK2.
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http://dx.doi.org/10.1093/jb/mvab047DOI Listing
April 2021

An anti-biofilm material: polysaccharides prevent the precipitation reaction of silver ions and chloride ions and lead to the synthesis of nano silver chloride.

Nanotechnology 2021 May 12;32(31). Epub 2021 May 12.

State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Qingdao, Shandong, 266237, People's Republic of China.

The formation of biofilm is one of the causes of bacterial pathogenicity and drug resistance. Recent studies have reported a variety of anti-biofilm materials and achieved good results. However, it is still very important to develop some materials with wider application scenarios or higher biofilm resistance. In this study, a new method to rapidly synthesize nano silver chloride with anti-biofilm activity is proposed. It is a generalizable method in which bacterial extracellular polysaccharides are used to adsorb silver ions, thereby inhibiting the formation of white large-size silver chloride precipitates, and then ultraviolet light is used to induce the synthesis of small-sized nano silver chloride. A variety of polysaccharides can be utilized in the synthesis of nano silver chloride particles. The generated complex was characterized by XRD, UV-vis, EDX, FTIR and TEM methods. Further, the novel complex was found to show highly effective anti-biofilm and bactericidal activity within the biosafety concentration. In view of the high stability of nano sliver chloride, we propose that the novel nano material has the potential as a long-term antibacterial material.
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http://dx.doi.org/10.1088/1361-6528/abf68eDOI Listing
May 2021

Vitamin D and iodine status was associated with the risk and complication of type 2 diabetes mellitus in China.

Open Life Sci 2021 18;16(1):150-159. Epub 2021 Feb 18.

Department of Endocrinology, Quanzhou First Hospital Affiliated to Fujian Medical University, No. 250, East Street, Licheng District, Quanzhou, 362300, Fujian, China.

The purpose of this study was to assess the relationship between 25-hydroxyvitamin D (25(OH)D), urinary iodine concentration (UIC), and type 2 diabetes mellitus (T2DM) risk and complications and to establish a model to predict T2DM in the general population. A total of 567 adults (389 T2DM patients and 178 controls) were enrolled, and the levels of 25(OH)D, iodine, and blood biochemical parameters were measured. Pearson's correlation analysis showed an inverse correlation between 25(OH)D level, UIC, and T2DM risk. Low 25(OH)D level was a risk factor for developing T2DM (OR, 0.81; 95% CI, 1.90-2.63; = 0.043) after adjustment for multiple risk factors. 25(OH)D level and UIC were inversely correlated with short-term and long-term glucose levels. 25(OH)D deficiency was also associated with a high incidence of T2DM complicated with thyroid dysfunction. A prediction model based on 25(OH)D, iodine status, and other risk factors was established and recommended to screen high-risk T2DM in the general population and provide early screening and timely treatment for them.
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http://dx.doi.org/10.1515/biol-2021-0019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968538PMC
February 2021

Whole Genome Sequencing in the Evaluation of Fetal Structural Anomalies: A Parallel Test with Chromosomal Microarray Plus Whole Exome Sequencing.

Genes (Basel) 2021 Mar 6;12(3). Epub 2021 Mar 6.

Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 201204, China.

Whole genome sequencing (WGS) is a powerful tool for postnatal genetic diagnosis, but relevant clinical studies in the field of prenatal diagnosis are limited. The present study aimed to prospectively evaluate the utility of WGS compared with chromosomal microarray (CMA) and whole exome sequencing (WES) in the prenatal diagnosis of fetal structural anomalies. We performed trio WGS (≈40-fold) in parallel with CMA in 111 fetuses with structural or growth anomalies, and sequentially performed WES when CMA was negative (CMA plus WES). In comparison, WGS not only detected all pathogenic genetic variants in 22 diagnosed cases identified by CMA plus WES, yielding a diagnostic rate of 19.8% (22/110), but also provided additional and clinically significant information, including a case of balanced translocations and a case of intrauterine infection, which might not be detectable by CMA or WES. WGS also required less DNA (100 ng) as input and could provide a rapid turnaround time (TAT, 18 ± 6 days) compared with that (31 ± 8 days) of the CMA plus WES. Our results showed that WGS provided more comprehensive and precise genetic information with a rapid TAT and less DNA required than CMA plus WES, which enables it as an alternative prenatal diagnosis test for fetal structural anomalies.
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http://dx.doi.org/10.3390/genes12030376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999180PMC
March 2021