Publications by authors named "Xiaoyu Jiang"

153 Publications

Effect of Glycerol on an N-Vinylpyrrolidone-Based Photopolymer for Transmission Holography.

Polymers (Basel) 2021 May 27;13(11). Epub 2021 May 27.

Department of Information Communication, Army Academy of Armored Forces, Beijing 100072, China.

N-vinylpyrrolidone (NVP) has a large molecular structure, so it is difficult to diffuse during holographic recording, especially at low spatial frequencies. We used glycerol to promote the diffusion of NVP, and successfully improved the holographic performance of the photopolymer at low spatial frequencies. As the concentration of glycerol increases, the holographic performance first increases and then remains stable. The optimal concentration of glycerol is 0.21 mol/L. At this concentration, the maximum diffraction efficiency of the photopolymer is 84%, the refractive index modulation is 1.95 × 10, and the photosensitive sensitivity is 7.91 × 10 cm/mJ. Compared with the control group, the maximum diffraction efficiency, maximum refractive index modulation and photosensitivity at low spatial frequencies (800 lp/mm) have increased by 11.19 times, 4.69 times and 1.71 times, respectively. Using the optimized photopolymer for transmission holographic recording and reproduction, we have obtained a clear and bright transmission hologram. The photopolymer modified with glycerol is expected to be applied to the fields of holography, diffractive optics, and so on.
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http://dx.doi.org/10.3390/polym13111754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198246PMC
May 2021

The Efficient Photocatalytic Degradation of Organic Pollutants on the MnFeO/BGA Composite under Visible Light.

Nanomaterials (Basel) 2021 May 13;11(5). Epub 2021 May 13.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, China.

The MnFeO/BGA (boron-doped graphene aerogel) composite was prepared by hydrothermal treatment of MnFeO particles, boric acid, and graphene oxide. When applied as a photo-Fenton catalyst for the degradation of rhodamine B, the MnFeO/BGA composite yielded a degradation efficiency much higher than the sum of those of individual MnFeO and BGA under identical experimental conditions, indicating a strong synergetic effect established between MnFeO and BGA. The catalytic degradation of rhodamine B was proved to follow pseudo first-order kinetics, and the apparent reaction rate constant on the MnFeO/BGA composite was calculated to be three- and seven-fold that on BGA and MnFeO, respectively. Moreover, the MnFeO/BGA composite also demonstrated good reusability and could be reused for four cycles without obvious loss of photocatalytic activity.
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http://dx.doi.org/10.3390/nano11051276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152228PMC
May 2021

Posterior Reversible Encephalopathy Syndrome Associated With Anlotinib: A Case Report and Literature Review.

Front Neurol 2021 6;12:546481. Epub 2021 May 6.

Department of Neurology and Neuroscience Center, The First Hospital of Jilin University, Changchun, China.

Posterior reversible encephalopathy syndrome (PRES) is a relatively rare clinical disease, characterized by reversible subcortical vasogenic edema. Here, we present the first reported case of PRES induced by anlotinib, a multi-target tyrosine kinase inhibitor. A 56-year-old female patient with lung adenocarcinoma and bone metastasis experienced hypertension and mental confusion when she received anti-angiogenesis treatment. PRES was diagnosed after magnetic resonance of the patient's brain revealed hyperintensities bilaterally around the cerebellum, pons, fronto-parieto-occipital areas, and corona radiate. Diffusion-weighted imaging showed hyperintensities bilaterally in the parieto-occipital cortical regions. Subsequently, the patient was diagnosed with PRES, and remission was achieved with anti-hypertensive drugs. Six cases of rare adverse effects induced by anlotinib were reviewed in the literature. Since anlotinib has been widely applied as a novel third-line treatment in patients with non-small-cell lung cancer, the association between PRES and anlotinib would benefit neurologists and oncologists in future diagnoses and treatment.
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http://dx.doi.org/10.3389/fneur.2021.546481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134686PMC
May 2021

Endoscopic Transcanal Push-Trough Myringoplasty for Different Types of Tympanic Membrane Perforations.

Otol Neurotol 2021 06;42(5):726-732

Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou.

Objective: This study aims to explore the feasibility of endoscopic transcanal push-trough myringoplasty (ETPM) for all types of tympanic membrane perforations (TMPs), and compare the outcomes with those of endoscopic type 1 tympanoplasty (ETT) with meatal flap elevation.

Study Design: A prospective, controlled study.

Methods: In the present study, inpatients with TMPs were divided into two groups according to the manner of tympanic membrane repair: one group received ETPM without raising the meatal flap, and the other received ETT. The operation duration, postoperative healing rate, visual analogue scale (VAS) of pain, and complication rates were compared between these two groups.

Results: Regardless of the size and location of the perforation, and its relationship with the malleus manubrium, myringoplasty can be completed using the ETPM method. The operation duration for different types of TMPs was shorter in the ETPM group than in the ETT group, and the difference was statistically significant (p<0.05). However, there was no statistical difference in the short-term healing rate (p>0.05) and pain VAS score (p>0.05) between these two groups. Furthermore, no intraoperative or postoperative complications occurred in both groups.

Conclusion: ETPM without raising the meatal flap can be applied for all types of TMPs, regardless of how large the perforation is, or where it is located. This can shorten the operation duration, and has a high healing rate comparable to ETT and mild postoperative pain. Mastering some essential surgical skills under the endoscope would be helpful to ensure the success of the surgery.
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http://dx.doi.org/10.1097/MAO.0000000000003029DOI Listing
June 2021

Synthesis and microwave absorption properties of coralloid core-shell structure NiS/[email protected]@MoS nanowires.

J Colloid Interface Sci 2021 Oct 24;599:262-270. Epub 2021 Apr 24.

School of Civil Engineering, Qingdao University of Technology, Qingdao 266033, People's Republic of China.

Herein, coralloid core-shell structure NiS/[email protected]@MoS nanowires were elaborately designed and successfully synthesized through a three-step route to obtain exceptional microwave absorption (MA) properties. Ni nanowires were first fabricated, and then used as the substrate to be coated with a layer of PPy. Ni chalcogenides were obtained by using Ni nanowire as sacrificial templates while growing MoS nanorods by hydrothermal method. Both the one-dimensional (1D) core-shell structure and the coralloid surface generated by MoS nanorods were beneficial for the attenuation of microwaves. After investigating the electromagnetic properties of different loading content absorbers (30 wt.%, 40 wt.% and 50 wt.%), it is found that the 50 wt.% loading absorber has the optimal MA performance. The minimum reflection loss (RL) value can reach -51.29 dB at 10.1 GHz with a thickness of 2.29 mm, and the corresponding effective absorption bandwidth (EAB, RL < -10 dB) can be up to 3.24 GHz. This research provides a reference for exploiting novel high-efficient 1D absorbers in the field of MA.
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http://dx.doi.org/10.1016/j.jcis.2021.04.107DOI Listing
October 2021

Sutimlimab in Cold Agglutinin Disease.

N Engl J Med 2021 04;384(14):1323-1334

From the Department of Hematology and Stem Cell Transplantation, West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, Essen, Germany (A.R.); Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Cà Granda Ospedale Maggiore Policlinico, Milan, Italy (W.B.); the Centre for Waldenström's Macroglobulinaemia and Related Conditions, University College London Hospitals National Health Service Foundation Trust, London (S.D.); the Thrombosis and Hemostasis Center, Saitama Medical University Hospital, Saitama, Japan (Y.M.); the Division of Hematology, MedStar Georgetown University Hospital, Washington, DC (C.M.B.); the Department of Internal Medicine, Henri-Mondor University Hospital, Assistance Publique-Hôpitaux de Paris, Université Paris-Est Créteil, Créteil, France (M.M.); the Division of Hematology, Massachusetts General Hospital, Harvard Medical School, Boston (D.J.K.), Bioverativ, Cambridge (J.F.), and Sanofi, Waltham (X.J., S.L., C.R., J.M.-A., W.H.) - all in Massachusetts; the Department of Clinical Pharmacology, Medical University of Vienna, Vienna (B.J.); and the Section for Hematology, Department of Medicine, Haukeland University Hospital, Bergen (T.H.A.T.), and the Department of Research and Innovation, Haugesund Hospital, Haugesund (S.B.) - both in Norway.

Background: Cold agglutinin disease is a rare autoimmune hemolytic anemia characterized by hemolysis that is caused by activation of the classic complement pathway. Sutimlimab, a humanized monoclonal antibody, selectively targets the C1s protein, a C1 complex serine protease responsible for activating this pathway.

Methods: We conducted a 26-week multicenter, open-label, single-group study to assess the efficacy and safety of intravenous sutimlimab in patients with cold agglutinin disease and a recent history of transfusion. The composite primary end point was a normalization of the hemoglobin level to 12 g or more per deciliter or an increase in the hemoglobin level of 2 g or more per deciliter from baseline, without red-cell transfusion or medications prohibited by the protocol.

Results: A total of 24 patients were enrolled and received at least one dose of sutimlimab; 13 patients (54%) met the criteria for the composite primary end point. The least-squares mean increase in hemoglobin level was 2.6 g per deciliter at the time of treatment assessment (weeks 23, 25, and 26). A mean hemoglobin level of more than 11 g per deciliter was maintained in patients from week 3 through the end of the study period. The mean bilirubin levels normalized by week 3. A total of 17 patients (71%) did not receive a transfusion from week 5 through week 26. Clinically meaningful reductions in fatigue were observed by week 1 and were maintained throughout the study. Activity in the classic complement pathway was rapidly inhibited, as assessed by a functional assay. Increased hemoglobin levels, reduced bilirubin levels, and reduced fatigue coincided with inhibition of the classic complement pathway. At least one adverse event occurred during the treatment period in 22 patients (92%). Seven patients (29%) had at least one serious adverse event, none of which were determined by the investigators to be related to sutimlimab. No meningococcal infections occurred.

Conclusions: In patients with cold agglutinin disease who received sutimlimab, selective upstream inhibition of activity in the classic complement pathway rapidly halted hemolysis, increased hemoglobin levels, and reduced fatigue. (Funded by Sanofi; CARDINAL ClinicalTrials.gov number, NCT03347396.).
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http://dx.doi.org/10.1056/NEJMoa2027760DOI Listing
April 2021

Local immune responses to VAA DNA vaccine against Listonella anguillarum in flounder (Paralichthys olivaceus).

Mol Immunol 2021 Jun 24;134:141-149. Epub 2021 Mar 24.

Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No. 1 Wenhai Road, Aoshanwei Town, Qingdao 266071, China. Electronic address:

The efficacy of DNA vaccine is associated closely with the expression of the antigen and the intensity of local immune responses. In our previous study, a recombinant DNA plasmid expressing the VAA protein (pVAA) of Listonella anguillarum has been proved to have a good protection against the infection of L. anguillarum. In the present study, the local immune responses eliciting by immunizing flounder with intramuscular (I.M.) injection of pVAA was investigated at the cellular and genetic level, the muscle at the injection site at 7th post vaccination day was sampled and analyzed by hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC), flow cytometry (FCM), RNA sequencing (RNA-Seq)-based transcriptomics and RT-qPCR. Then variations on the specific antibodies in serum at 1st-6th post vaccination week and the relative percent survival rate (RPS) at following 14 days after challenge were measured. The H&E results showed that inflammatory cells and immune cells significantly increased at the injection site. The IHC using monoclonal antibody against T cell markers revealed that both CD4-1 and CD4-2 T lymphocytes were recruited to the injection site and FCM results showed that the proportion of CD4-1 cells in pVAA immunized group was 28.6 %, in the control group was 8.7 %, and that of CD4-2 cells in two groups was 21.2 % and 8.5 %, respectively. These results indicating that the proportion of CD4 cells in the immune group was significantly increased compared with the control group. Moreover, there were 2551 genes differently expressed in pVAA immunized group, KEGG analysis showed the genes involved in the signal transduction and immune system, and surface markers for B-cells genes, T-cells and antigen presenting cells (APCs) genes were highly upregulated, suggesting the activation of the systemic immune responses. Antibody specific anti-L. anguillarum or anti-rVAA antibodies were significantly induced at 2nd post-immunization week, that reached a peak at 4-5th week. RPS in pVAA group was 53.85±3.64 %. In conclusion, pVAA induced effective local immune responses and then the systematic response. This probably is the main contribution of pVAA to effective protection against L. anguillarum.
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http://dx.doi.org/10.1016/j.molimm.2021.03.012DOI Listing
June 2021

The complete chloroplast genome sequence of (Lour.) G. Don (Styracaceae).

Mitochondrial DNA B Resour 2021 Mar 11;6(3):774-775. Epub 2021 Mar 11.

Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, Nanjing Forestry University, Nanjing, China.

(Lour.) G. Don, is a deciduous species of Styracaceae with beautiful shape, drooping flowers, and blooming like snow. Here, we characterized the complete chloroplast (cp) genome of using next generation sequencing. The circular complete cp genome of is 157,893 bp in length, containing a large single-copy (LSC) region of 87,512 bp, and a small single-copy (SSC) region of 18,285 bp. It comprises 136 genes, including eight rRNA genes, 37 tRNAs genes, 90 protein-coding genes, and one pseudo gene. The GC content of cp genome is 36.96%. The phylogenetic analysis suggests that is a sister species to in Styracaceae.
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http://dx.doi.org/10.1080/23802359.2021.1882351DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954495PMC
March 2021

Emergence of one novel reassortment H3N8 avian influenza virus in China, originating from North America and Eurasia.

Infect Genet Evol 2021 Jul 24;91:104782. Epub 2021 Feb 24.

National Avian Influenza Para-Reference Laboratory (Guangzhou), College of Veterinary Medicine,South China Agricultural University, Guangzhou 510642, China; Key Laboratory of Zoonosis, Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Aairs, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China; Key Laboratory of Zoonoses Prevention and Control of Guangdong Province, Guangzhou 510642, China. Electronic address:

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http://dx.doi.org/10.1016/j.meegid.2021.104782DOI Listing
July 2021

Promoting Water Conservation Based on the Matching Effect of Regulatory Focus and Emotion.

Int J Environ Res Public Health 2021 02 9;18(4). Epub 2021 Feb 9.

School of Media Studies and Humanities, Zhejiang University City College, Hangzhou 310015, China.

This study aimed to examine the effects of regulatory focus and emotions on water-saving information dissemination. The findings revealed that when water-saving information is framed with a prevention focus, sad emotion fosters more active willingness to engage with the information dissemination than cheerful emotion. However, a promotion focus coupled with cheerfulness is slightly more persuasive than a promotion focus coupled with sadness. Furthermore, compared to the individuals in the nonfit group of emotions who had a regulatory focus, the individuals in the fit group formed a more favorable water-saving attitude and demonstrated a slightly higher willingness to disseminate water-saving information. This article is the first to contribute to exploring the dissemination of water-saving information from the perspective of the interactive effect of individual cognitive motivation and emotion.
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http://dx.doi.org/10.3390/ijerph18041680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916247PMC
February 2021

The complete chloroplast genome sequence of (J.Anthony) Rehder (Styracaceae).

Mitochondrial DNA B Resour 2021 Jan 21;6(1):205-207. Epub 2021 Jan 21.

Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, Nanjing Forestry University, Nanjing, China.

(J.Anthony) Rehder, which plays an important role in ecology and economy, is a deciduous species of Styracaceae. The authors sequenced, assembled, and annotated the chloroplast (cp) genome of using the sequencing data from Illumina Novaseq platform in this study. The complete cp genome of is 159,320 bp in length, including a large single-copy (LSC) region of 87,795 bp, and a small single-copy (SSC) region of 18,989 bp. It contains 130 genes, including 37 tRNA genes, 8 rRNA genes, and 85 protein-coding genes. The overall GC content of chloroplast genome is 36.66%. The phylogenetic analysis suggests that is a sister species to in Styracaceae.
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http://dx.doi.org/10.1080/23802359.2020.1860695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832471PMC
January 2021

The complete chloroplast genome sequence of Hu et S.Y. Liang ().

Mitochondrial DNA B Resour 2020 Sep 17;5(3):3363-3365. Epub 2020 Sep 17.

Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, Nanjing Forestry University, Nanjing, PR China.

Hu et S.Y. Liang, is one of the few evergreen species of with fragrant flowers. Here, we characterized the complete chloroplast (cp) genome of using next-generation sequencing. The circular complete cp genome of is 158,502 bp in length, containing a large single-copy (LSC) region of 87,817 bp, and a small single-copy (SSC) region of 18,001 bp. It comprises 132 genes, including 8 genes, 37 genes, and 87 protein-coding genes. The GC content of cp genome is 36.93%. The phylogenetic analysis suggests that is a sister species to in .
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http://dx.doi.org/10.1080/23802359.2020.1821822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782962PMC
September 2020

Nanoscale assembly line composed of dual DNA-machines enabling sensitive microRNA detection using upconversion nanoparticles probes.

J Pharm Biomed Anal 2021 Feb 25;195:113842. Epub 2020 Dec 25.

The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, School of Pharmacy, Fujian Medical University, Fuzhou, 350122, PR China. Electronic address:

DNA machines are smart artificial devices that perform well-organized DNA hybridization reactions or nanoscale mechanical movements. Herein, a nanoscale assembly line composing of dual DNA machines is meticulously designed by coupling a catalytic hairpin assembly (CHA)-based machine with a 3D DNA walker machine. Equipped with upconversion nanoparticles (UCNPs) as signal tags, the dual DNA machines-based assembly line (DDMAL) can efficiently amplify the fluorescent signal of target recognition event, enabling sensitive detection of microRNA (miRNA). In detail, once activated by target miRNA-21, the CHA machine is initiated to constantly produce a single-stranded DNA (named binding DNA) via the strand displacement reaction. The binding DNA as a trigger factor can initiate the DNA walker machine by linking a walking strand DNA with an anchor strand DNA immobilized on the surface of magnetic beads (MBs). The movement of walking strand on the surface of MBs is then driven by Mn-dependent DNAzyme formed through the hybridization of walking strand with a UCNPs-linked substrate strand. The DNAzyme-catalyzed cleavage of substrate strand is accompanied by the release of numerous UCNPs from MBs. By measuring the fluorescent signal of released UCNPs after the magnetic separation, target miRNA-21 can be detected by the DDMAL system in a linear range from 1.0 fM to 10 nM, with a limit of detection (LOD) of 0.62 fM (3σ). Moreover, the practicability of DDMAL system was demonstrated by using it to evaluate the expression levels of miRNA-21 in cell lines and assay miRNA-21 in human serum.
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http://dx.doi.org/10.1016/j.jpba.2020.113842DOI Listing
February 2021

MR cell size imaging with temporal diffusion spectroscopy.

Magn Reson Imaging 2021 04 16;77:109-123. Epub 2020 Dec 16.

Institute of Imaging Science, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Radiology and Radiological Sciences, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37232, USA; Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 37232, USA. Electronic address:

Cytological features such as cell size and intracellular morphology provide fundamental information on cell status and hence may provide specific information on changes that arise within biological tissues. Such information is usually obtained by invasive biopsy in current clinical practice, which suffers several well-known disadvantages. Recently, novel MRI methods such as IMPULSED (imaging microstructural parameters using limited spectrally edited diffusion) have been developed for direct measurements of mean cell size non-invasively. The IMPULSED protocol is based on using temporal diffusion spectroscopy (TDS) to combine measurements of water diffusion over a wide range of diffusion times to probe cellular microstructure over varying length scales. IMPULSED has been shown to provide rapid, robust, and reliable mapping of mean cell size and is suitable for clinical imaging. More recently, cell size distributions have also been derived by appropriate analyses of data acquired with IMPULSED or similar sequences, which thus provides MRI-cytometry. This review summarizes the basic principles, practical implementations, validations, and example applications of MR cell size imaging based on TDS and demonstrates how cytometric information can be used in various applications. In addition, the limitations and potential future directions of MR cytometry are identified including the diagnosis of nonalcoholic steatohepatitis of the liver and the assessment of treatment response of cancers.
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http://dx.doi.org/10.1016/j.mri.2020.12.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878439PMC
April 2021

Chemical exchange rotation transfer imaging of phosphocreatine in muscle.

NMR Biomed 2021 02 7;34(2):e4437. Epub 2020 Dec 7.

Vanderbilt University Institute of Imaging Science, Nashville, Tennessee.

In chemical exchange saturation transfer (CEST) imaging, the signal at 2.6 ppm from the water resonance in muscle has been assigned to phosphocreatine (PCr). However, this signal has limited specificity for PCr since the signal is also sensitive to exchange with protein and macromolecular protons when using some conventional quantification methods, and will vary with changes in the water longitudinal relaxation rate. Correcting for these effects while maintaining reasonable acquisition times is challenging. As an alternative approach to overcome these problems, here we evaluate chemical exchange rotation transfer (CERT) imaging of PCr in muscle at 9.4 T. Specifically, the CERT metric, AREX at 2.6 ppm, was measured in solutions containing the main muscle metabolites, in tissue homogenates with controlled PCr content, and in vivo in rat leg muscles. PCr dominates CERT metrics around 2.6 ppm (although with nontrivial confounding baseline contributions), indicating that CERT is well-suited to PCr specific imaging, and has the added benefit of requiring a relatively small number of acquisitions.
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http://dx.doi.org/10.1002/nbm.4437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902410PMC
February 2021

Affordable automated phenotypic antibiotic susceptibility testing method based on a contactless conductometric sensor.

Sci Rep 2020 12 4;10(1):21216. Epub 2020 Dec 4.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.

User-friendly phenotypic antibiotic susceptibility testing (AST) methods are urgently needed in many fields including clinical medicine, epidemiological studies and drug research. Herein, we report a convenient and cost-effective phenotypic AST method based on online monitoring bacterial growth with a developed 8-channel contactless conductometric sensor (CCS). Using E. coli and V. parahaemolyticus as microorganism models, as well as enoxacin, florfenicol, ampicillin, kanamycin and sulfadiazine as antibiotic probes. The minimum inhibitory concentration (MIC) determination was validated in comparison with standard broth microdilution (BMD) assay. The total essential agreements between the CCS AST assays and the reference BMD AST assays are 68.8-92.3%. The CCS has an approximate price of $9,000 (USD). Requiring neither chemical nor biotic auxiliary materials for the assay makes the cost of each sample < $1. The MICs obtained with the automated CCS AST assays are more precise than those obtained with the manual BMD. Moreover, in 72 percent of the counterpart, the MICs obtained with the CCS AST assays are higher than that obtained with the BMD AST assays. The proposed CCS AST method has advantages in affordability, accuracy, sensitivity and user-friendliness.
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http://dx.doi.org/10.1038/s41598-020-77938-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718250PMC
December 2020

Conditional expression of HGAL leads to the development of diffuse large B-cell lymphoma in mice.

Blood 2021 Apr;137(13):1741-1753

Experimental Therapeutics and Translational Oncology Program, Instituto de Biología Molecular y Celular del Cáncer, Centro Superior de Investigaciones Científicas (CSIC)/Universidad de Salamanca (USAL), Salamanca, Spain.

Diffuse large B-cell lymphomas (DLBCLs) are clinically and genetically heterogeneous tumors. Deregulation of diverse biological processes specific to B cells, such as B-cell receptor (BCR) signaling and motility regulation, contribute to lymphomagenesis. Human germinal center associated lymphoma (HGAL) is a B-cell-specific adaptor protein controlling BCR signaling and B lymphocyte motility. In normal B cells, it is expressed in germinal center (GC) B lymphocytes and promptly downregulated upon further differentiation. The majority of DLBCL tumors, primarily GC B-cell types, but also activated types, express HGAL. To investigate the consequences of constitutive expression of HGAL in vivo, we generated mice that conditionally express human HGAL at different stages of hematopoietic development using 3 restricted Cre-mediated approaches to initiate expression of HGAL in hematopoietic stem cells, pro-B cells, or GC B cells. Following immune stimulation, we observed larger GCs in mice in which HGAL expression was initiated in GC B cells. All 3 mouse strains developed DLBCL at a frequency of 12% to 30% starting at age 13 months, leading to shorter survival. Immunohistochemical studies showed that all analyzed tumors were of the GC B-cell type. Exon sequencing revealed mutations reported in human DLBCL. Our data demonstrate that constitutive enforced expression of HGAL leads to DLBCL development.
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http://dx.doi.org/10.1182/blood.2020004996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020264PMC
April 2021

Rapid detection of cytochrome cd1-containing nitrite reductase encoding gene nirS of denitrifying bacteria with loop-mediated isothermal amplification assay.

Sci Rep 2020 10 5;10(1):16484. Epub 2020 Oct 5.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.

The cytochrome cd1-containing nitrite reductase, nirS, plays an important role in biological denitrification. Consequently, investigating the presence and abundance of nirS is a commonly used approach to understand the distribution and potential activity of denitrifying bacteria, in addition to denitrifier communities. Herein, a rapid method for detecting nirS gene with loop-mediated isothermal amplification (LAMP) was developed, using Pseudomonas aeruginosa PAO1 (P. aeruginosa PAO1) as model microorganism to optimize the assay. The LAMP assay relied on a set of four primers that were designed to recognize six target sequence sites, resulting in high target specificity. The limit of detection for the LAMP assay under optimized conditions was 1.87 pg/reaction of genomic DNA, which was an order of magnitude lower than that required by conventional PCR assays. Moreover, it was validated that P. aeruginosa PAO1 cells as well as genomic DNA could be directly used as template. Only 1 h was needed from the addition of bacterial cells to the reaction to the verification of amplification success. The nirS gene of P. aeruginosa PAO1 in spiked seawater samples could be detected with both DNA-template based LAMP assay and cell-template based LAMP assay, demonstrating the practicality of in-field use.
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http://dx.doi.org/10.1038/s41598-020-73304-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536394PMC
October 2020

Endoscope-Assisted Surgery of the Elongated Styloid Process Using the Retroauricular Approach: An Anatomic Study for Clinical Application.

J Oral Maxillofac Surg 2021 Mar 28;79(3):622-628. Epub 2020 Aug 28.

Professor, Department of Otolaryngology, Head and Neck Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China; and Professor, Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China. Electronic address:

Purpose: Surgical shortening of the styloid process (SP) mainly involves intraoral and transcervical approaches. A retroauricular incision was performed by our surgical team in endoscope-assisted shortening of the SP. This study aimed to clarify the important anatomic landmarks and adjacent structures around the SP through a retroauricular approach.

Methods: Fifteen fresh corpses (30 sides) were dissected via a retroauricular approach, and indexes were measured.

Results: The great auricular nerve (GAN) was divided into the anterior ear branch, lobe branch, and posterior ear branch. The distance from the branch of the GAN to the root of the ear lobe was 21.96 ± 2.55 mm. In the space around the SP, the vertical distance from the junction of the diabetic posterior belly and the mastoid tip to the SP was found to be 12.29 ± 2.46 mm, with a total distance between the skin in front of the mastoid and the facial nerve of 21.63 ± 3.27 mm. The distance between the facial nerve across the SP and the root of the SP was 11.93 ± 2.32 mm.

Conclusions: The retroauricular incision starts from the level of the notch between the tragus and extends backward in an arc to avoid injury to the retroauricular branch of the GAN. The posterior fascia of the parotid gland and the leading edge of the sternocleidomastoid muscle, posterior belly of the digastric muscle, and styloid hyoid muscle are regarded as landmarks for the SP.
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http://dx.doi.org/10.1016/j.joms.2020.08.032DOI Listing
March 2021

MRI-cytometry: Mapping nonparametric cell size distributions using diffusion MRI.

Magn Reson Med 2021 02 16;85(2):748-761. Epub 2020 Sep 16.

Vanderbilt University Institute of Imaging Science, Vanderbilt University Medical Center, Nashville, TN, USA.

Purpose: This report introduces and validates a new diffusion MRI-based method, termed MRI-cytometry, which can noninvasively map intravoxel, nonparametric cell size distributions in tissues.

Methods: MRI was used to acquire diffusion MRI signals with a range of diffusion times and gradient factors, and a model was fit to these data to derive estimates of cell size distributions. We implemented a 2-step fitting method to avoid noise-induced artificial peaks and provide reliable estimates of tumor cell size distributions. Computer simulations in silico, experimental measurements on cultured cells in vitro, and animal xenografts in vivo were used to validate the accuracy and precision of the method. Tumors in 7 patients with breast cancer were also imaged and analyzed using this MRI-cytometry approach on a clinical 3 Tesla MRI scanner.

Results: Simulations and experimental results confirm that MRI-cytometry can reliably map intravoxel, nonparametric cell size distributions and has the potential to discriminate smaller and larger cells. The application in breast cancer patients demonstrates the feasibility of direct translation of MRI-cytometry to clinical applications.

Conclusion: The proposed MRI-cytometry method can characterize nonparametric cell size distributions in human tumors, which potentially provides a practical imaging approach to derive specific histopathological information on biological tissues.
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http://dx.doi.org/10.1002/mrm.28454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722100PMC
February 2021

On-stream mineral identification of tailing slurries of tungsten NIR and XRF data fusion measurement techniques.

Anal Methods 2020 07;12(25):3296-3307

School of Mechanical and Electronic Engineering, East China University of Technology, Nanchang, 330013, China.

Mineral flotation processes are controlled by monitoring the grade of the present minerals. The economy of the flotation process can be significantly improved by on-line analysis of minerals in a slurry. However, online and quantitative mineral identification of slurries is challenging. Industrial developers are demanding novel ideas enabling differentiation between minerals with similar elemental contents, such as scheelite and fluorite or gangue minerals, since they have different flotation properties. The primary focus of this research is the measurement of mineral contents from the elemental concentrations acquired by an on-stream slurry analyser based on X-ray fluorescence (XRF) and near-infrared spectroscopy (NIR). In this work, the samples in the test were obtained from a tungsten dressing plant. It is vital to master the mineral grade for controlling the flotation plant. The XRF parameters were optimised by Monte Carlo simulation, and the XRF and NIR data fusion was discussed. A multivariate statistical method called the least squares support vector machine (LS-SVM) was employed to perform the element-to-mineral conversion. The results show that such data integrations enable on-stream and quantitative identification of slurry mineral contents, especially for scheelite, wolframite, fluorite and calcite, which are essential minerals in tungsten ore beneficiation. This technique can lead to many benefits, such as rapid control of concentrate quality, enhanced recovery and savings in money, time, energy and workforce.
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http://dx.doi.org/10.1039/d0ay00322kDOI Listing
July 2020

Optimization and numerical evaluation of multi-compartment diffusion MRI using the spherical mean technique for practical multiple sclerosis imaging.

Magn Reson Imaging 2020 12 6;74:56-63. Epub 2020 Sep 6.

Institute of Imaging Science, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Radiology and Radiological Sciences, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37232, USA; Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 37232, USA. Electronic address:

Background: The multi-compartment diffusion MRI using the spherical mean technique (SMT) has been suggested to enhance the pathological specificity to tissue injury in multiple sclerosis (MS) imaging, but its accuracy and precision have not been comprehensively evaluated.

Methods: A Cramer-Rao Lower Bound method was used to optimize an SMT protocol for MS imaging. Finite difference computer simulations of spins in packed cylinders were then performed to evaluate the influences of five realistic pathological features in MS lesions: axon diameter, axon density, free water fraction, axonal crossing, dispersion, and undulation.

Results: SMT derived metrics can be biased by some confounds of pathological variations, such as axon size and free water fraction. However, SMT in general provides valuable information to characterize pathological features in MS lesions with a clinically feasible protocol.

Conclusion: SMT may be used as a practical MS imaging method and should be further improved in clinical MS imaging.
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http://dx.doi.org/10.1016/j.mri.2020.09.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669580PMC
December 2020

View-flipping effect reduction and reconstruction visualization enhancement for EPISM based holographic stereogram with optimized hogel size.

Sci Rep 2020 Aug 10;10(1):13492. Epub 2020 Aug 10.

Department of Information Communication, Academy of Army Armored Forces, Beijing, 100072, China.

To reduce the view-flipping effect and enhance the viewing resolution, the modulation characteristics of the hogel based holographic stereogram is constructed and validated. The performance of the view-flipping effect is analyzed, and the results indicate that decreasing the size of hogel is beneficial to the reduction of the view flipping, however, which will result in significant diffraction effect which can degrade the reconstruction quality. Furthermore, a diffraction-limited imaging model of the hogel based holographic stereogram is established, where both the limited aperture of the hogel and the defocused aberration of the object point are introduced, and the effective resolvable size of the reconstructed image point is simulated. The theory shows that there is an optimal hogel size existed for the certain depth of scene. Both the numerical and optical experiments are implemented, and the results are well agreed with the theoretical prediction, which demonstrates that the view-flipping reduction and reconstruction visualization enhancement for EPISM based holographic stereogram can be achieved when the proper size of hogel is utilized.
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http://dx.doi.org/10.1038/s41598-020-70256-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417599PMC
August 2020

ALKBH1 deficiency leads to loss of homeostasis in human diploid somatic cells.

Protein Cell 2020 09;11(9):688-695

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.

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http://dx.doi.org/10.1007/s13238-020-00744-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7452965PMC
September 2020

MRI of tumor T cell infiltration in response to checkpoint inhibitor therapy.

J Immunother Cancer 2020 06;8(1)

Vanderbilt University Institute of Imaging Science, Nashville, TN 37232, United States

Background: Immune checkpoint inhibitors, the most widespread class of immunotherapies, have demonstrated unique response patterns that are not always adequately captured by traditional response criteria such as the Response Evaluation Criteria in Solid Tumors or even immune-specific response criteria. These response metrics rely on monitoring tumor growth, but an increase in tumor size and/or appearance after starting immunotherapy does not always represent tumor progression, but also can be a result of T cell infiltration and thus positive treatment response. Therefore, non-invasive and longitudinal monitoring of T cell infiltration are needed to assess the effects of immunotherapies such as checkpoint inhibitors. Here, we proposed an innovative concept that a sufficiently large influx of tumor infiltrating T cells, which have a smaller diameter than cancer cells, will change the diameter distribution and decrease the average size of cells within a volume to a degree that can be quantified by non-invasive MRI.

Methods: We validated our hypothesis by studying tumor response to combination immune-checkpoint blockade (ICB) of anti-PD-1 and anti-CTLA4 in a mouse model of colon adenocarcinoma (MC38). The response was monitored longitudinally using Imaging Microstructural Parameters Using Limited Spectrally Edited Diffusion (IMPULSED), a diffusion MRI-based method which has been previously shown to non-invasively map changes in intracellular structure and cell sizes with the spatial resolution of MRI, in cell cultures and in animal models. Tumors were collected for immunohistochemical and flow cytometry analyzes immediately after the last imaging session.

Results: Immunohistochemical analysis revealed that increased T cell infiltration of the tumors results in a decrease in mean cell size (eg, a 10% increase of CD3 T cell fraction results a ~1 µm decrease in the mean cell size). IMPULSED showed that the ICB responders, mice with tumor volumes were less than 250 mm or had tumors with stable or decreased volumes, had significantly smaller mean cell sizes than both Control IgG-treated tumors and ICB non-responder tumors.

Conclusions: IMPULSED-derived cell size could potentially serve as an imaging marker for differentiating responsive and non-responsive tumors after checkpoint inhibitor therapies, a current clinical challenge that is not solved by simply monitoring tumor growth.
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http://dx.doi.org/10.1136/jitc-2019-000328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312343PMC
June 2020

Mapping hepatocyte size in vivo using temporal diffusion spectroscopy MRI.

Magn Reson Med 2020 11 25;84(5):2671-2683. Epub 2020 Apr 25.

Institute of Imaging Science, Vanderbilt University, Nashville, Tennessee, USA.

Purpose: The goal of this study is to implement a noninvasive method for in vivo mapping of hepatocyte size. This method will have a broad range of clinical and preclinical applications, as pathological changes in hepatocyte sizes are relevant for the accurate diagnosis and assessments of treatment response of liver diseases.

Methods: Building on the concepts of temporal diffusion spectroscopy in MRI, a clinically feasible imaging protocol named IMPULSED (Imaging Microstructural Parameters Using Limited Spectrally Edited Diffusion) has been developed, which is able to report measurements of cell sizes noninvasively. This protocol acquires a selected set of diffusion imaging data and fits them to a model of water compartments in tissues to derive robust estimates of the cellular structures that restrict free diffusion. Here, we adapt and further develop this approach to measure hepatocyte sizes in vivo. We validated IMPULSED in livers of mice and rats and implemented it to image healthy human subjects using a clinical 3T MRI scanner.

Results: The IMPULSED-derived mean hepatocyte sizes for rats and mice are about 15-20 µm and agree well with histological findings. Maps of mean hepatocyte size for humans can be achieved in less than 15 minutes, a clinically feasible scan time.

Conclusion: Our results suggest that this method has potential to overcome major limitations of liver biopsy and provide noninvasive mapping of hepatocyte sizes in clinical applications.
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http://dx.doi.org/10.1002/mrm.28299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7402009PMC
November 2020

[Application of adhesive materials in biomedicine: progress and prospects].

Sheng Wu Gong Cheng Xue Bao 2019 Dec;35(12):2386-2400

Materials and Physical Biology Division, School of Physical Science and Technology, Shanghai Tech University, Shanghai 201210, China.

As an important auxiliary material, adhesive materials have many important applications in various fields including but not limited to industrial packaging, marine engineering, and biomedicine. Naturally occurring adhesives such as mussel foot proteins are usually biocompatible and biodegradable, but their limited sources and poor mechanical properties in physiological conditions have limited their widespread uses in biomedical field. Inspired by the underwater adhesion phenomenon of natural organisms, a series of biomimetic adhesive materials have been developed through chemical or bioengineering approaches. Notably, some of those synthetic adhesives have exhibited great promise for medical applications in terms of their biocompatibility, biodegradability, strong tissue adhesion and many other attractive functional properties. As natural adhesive materials possess distinctive "living" attributes such as environmental responsiveness, self-regeneration and autonomous repairs, the development of various biologically inspired and biomimetic adhesive materials using natural adhesives as blueprints will thus be of keen and continuous interest in the future. The emerging field of synthetic biology will likely provide new opportunities to design living glues that recapitulate the dynamic features of those naturally occurring adhesives.
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http://dx.doi.org/10.13345/j.cjb.190366DOI Listing
December 2019

Centered-camera-based effective perspective images' segmentation and mosaicking method for full-parallax holographic stereogram printing.

Appl Opt 2019 Nov;58(33):9112-9130

The centered-camera-based effective perspective images' segmentation and mosaicking (CCEPISM) method is proposed to improve the previous EPISM-based printing of full-parallax holographic stereograms. The idea of pixel mapping from target camera images to centered camera images is analyzed, and backward pixel mapping as well as forward pixel mapping are modeled and formulated to transform the perspective images captured by different camera strategies. The principle of the proposed CCEPISM is introduced in detail along with its specific implementation. The experimental results validate the proposed method and demonstrate that CCEPISM is an effective method for printing full-parallax holographic stereograms, and the quality of optical reconstruction is the same as that of EPISM-based holographic stereograms. The whole 3D scene is captured by a centered camera image, and the pixel waste caused by simple camera capture and the number of sampled perspective images is reduced significantly.
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http://dx.doi.org/10.1364/AO.58.009112DOI Listing
November 2019

Conductometric sensor for viable Escherichia coli and Staphylococcus aureus based on magnetic analyte separation via aptamer.

Mikrochim Acta 2019 12 12;187(1):43. Epub 2019 Dec 12.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.

A method is described to determine viable populations of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The method employs aptamer-magnetic separation combined with resistivity based detection. The bacteria were separated by means of aptamer-functionalized magnetic beads. They were then quantified by measuring their growth kinetics through time-dependent conductivity changes of culture media. The time-course of growth was logged by real-time and contactless measurements that yielded starting concentrations from the duration of lag intervals prior to the log phase of growth. In pure water samples, the linear ranges for measuring E. coli and S. aureus cells are 2.5 × 10-2.5 × 10 CFU·mL and 4.1 × 10-4.1 × 10 CFU·mL, respectively. In spiked tap water samples, the lower limits of detection are 2.3 × 10 CFU·mL and 4.0 × 10 CFU·mL for E. coli and S. aureus, with recoveries of 87.0-108.7% and 92.5-105.0%, respectively. The relative standard deviation of these measurements (10.0%) is below that of plate counting method (13.9%). The presence of micro/nanoparticles such as magnetic beads or selenium nanoparticles in the culture media does not interfere, unlike in case of automatted optical density monitoring. The E. coli and S. aureus cells captured on the aptamer-functionalized magnetic beads can be directly tested for their susceptibility to antibiotics. The process of magnetic separation and determination of load burden requires neither bulky, sophisticated equipment nor expensive reagents. Graphical abstractAptamer-functionalized magnetic beads are used to selectively capture and separate E. coli and S. aureus cells in aqueous samples. They are directly transferred to a multichannel conductometric sensor for the quantification of viable bacteria via automated monitoring of their growth kinetics.
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http://dx.doi.org/10.1007/s00604-019-3880-0DOI Listing
December 2019

Magnetic resonance imaging of mean cell size in human breast tumors.

Magn Reson Med 2020 06 25;83(6):2002-2014. Epub 2019 Nov 25.

Institute of Imaging Science, Vanderbilt University Medical Center, Nashville, Tennessee.

Purpose: Cell size is a fundamental characteristic of all tissues, and changes in cell size in cancer reflect tumor status and response to treatments, such as apoptosis and cell-cycle arrest. Unfortunately, cell size can currently be obtained only by pathological evaluation of tumor tissue samples obtained invasively. Previous imaging approaches are limited to preclinical MRI scanners or require relatively long acquisition times that are impractical for clinical imaging. There is a need to develop cell-size imaging for clinical applications.

Methods: We propose a clinically feasible IMPULSED (imaging microstructural parameters using limited spectrally edited diffusion) approach that can characterize mean cell sizes in solid tumors. We report the use of a combination of pulse sequences, using different gradient waveforms implemented on clinical MRI scanners and analytical equations based on these waveforms to analyze diffusion-weighted MRI signals and derive specific microstructural parameters such as cell size. We also describe comprehensive validations of this approach using computer simulations, cell experiments in vitro, and animal experiments in vivo and demonstrate applications in preoperative breast cancer patients.

Results: With fast acquisitions (~7 minutes), IMPULSED can provide high-resolution (1.3 mm in-plane) mapping of mean cell size of human tumors in vivo on clinical 3T MRI scanners. All validations suggest that IMPULSED provides accurate and reliable measurements of mean cell size.

Conclusion: The proposed IMPULSED method can assess cell-size variations in tumors of breast cancer patients, which may have the potential to assess early response to neoadjuvant therapy.
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http://dx.doi.org/10.1002/mrm.28056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047520PMC
June 2020