Publications by authors named "Xiaoying Liu"

288 Publications

Electroacupuncture at GV20‑GB7 regulates mitophagy to protect against neurological deficits following intracerebral hemorrhage via inhibition of apoptosis.

Mol Med Rep 2021 Jul 6;24(1). Epub 2021 May 6.

Department of Clinical Medicine, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China.

The acupuncture penetrating line of Baihui (GV20) to Qubin (GB7) spans the parietal, frontal and temporal lobes. The present study aimed to elucidate the mechanism by which electroacupuncture (EA) at GV20‑GB7 regulates mitophagy in intracerebral hemorrhage (ICH) and whether it serves a neuroprotective role. A whole blood‑induced ICH model was used. Mitophagy‑regulating proteins, including BCL/adenovirus E1B 19 kDa‑interacting protein 3 (BNIP3), PTEN‑induced putative kinase 1 (PINK1), Parkin and apoptosis‑associated proteins were detected by western blotting; autophagy following ICH was evaluated by immunofluorescent techniques; morphological characteristics of mitophagy were observed using transmission electron microscopy; and TUNEL assay was performed to determine the number of apoptotic cells. Immunohistochemistry was used to detect p53 expression. The protective role of EA (GV20‑GB7) via enhanced mitophagy and suppressed apoptosis in ICH was further confirmed by decreased modified neurological severity score. The results showed that EA (GV20‑GB7) treatment upregulated mitochondrial autophagy following ICH and inhibited apoptotic cell death. The mechanism underlying EA (GV20‑GB7) treatment may involve inhibition of p53, an overlapping protein of autophagy and apoptosis. EA (GV20‑GB7) treatment decreased neurobehavioral deficits following ICH but pretreatment with 3‑methyladenine counteracted the beneficial effects of EA (GV20‑GB7) treatment. In conclusion, EA (GV20‑GB7) improved recovery from ICH by regulating the balance between mitophagy and apoptosis.
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http://dx.doi.org/10.3892/mmr.2021.12131DOI Listing
July 2021

Analysis of transient response of the human foot based on the finite element method.

Technol Health Care 2021 Apr 21. Epub 2021 Apr 21.

College of Mechanical Engineering and Automation, Huaqiao University, Xiamen, Fujian, China.

Background: The foot is an important part of the human body. Its functions are mainly walking and load-bearing. It also keeps the human body stable and absorbs ground vibrations to protect important human organs.

Objective: Many researchers use finite element methods to study the biomechanics of the foot. However, current studies on the finite element of the foot are based on the stress and displacement response analysis of the foot under static or quasi-static conditions, ignoring the movement process of the foot and the impact of vibration. Moreover, the joint application of energy method and finite element analysis in foot biomechanics is rarely reported.

Methods: In this paper, to obtain the foot energy transfer process, the transient response of the foot under neutral position is analyzed based on the energy method.

Results: The results show that: (1) In this model, the energy analysis follows the conservation of energy, which indicates that the transient response analysis has obtained a reasonable response. (2) When the foot touches the ground, the strain energy of the calcaneus, second metatarsal and third metatarsal is relatively large, which is consistent with the main stress concentration area of the plantar. (3) The gravity of the human body is mainly transmitted through the talus to the calcaneus, while the effect of transmittal through the scaphoid to the cuneiform bone and metatarsal is weak.

Conclusion: This study can not only more clearly and intuitively reflect the energy transfer and source of various skeletal foreheads in the foot, but also provide a new research idea for the study of foot biomechanics.
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http://dx.doi.org/10.3233/THC-202673DOI Listing
April 2021

Association and interaction effect of UCP2 gene polymorphisms and dietary factors with congenital heart diseases in Chinese Han population.

Sci Rep 2021 Apr 22;11(1):8699. Epub 2021 Apr 22.

Department of Epidemiology and Health Statistics, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha, 410078, Hunan, China.

Congenital heart diseases (CHDs) are the most common birth defects and the leading cause of non-infectious deaths in infants, with an unknown etiology. We aimed to assess the association of genetic variations in UCP2 gene, dietary factors, and their interactions with the risk of CHDs in offspring. The hospital-based case-control study included 464 mothers of children with CHDs and 504 mothers of healthy children. The exposures of interest were maternal dietary factors in early pregnancy and UCP2 genetic variants. Logistic regression analyses were used to assess the association and interaction of UCP2 gene and dietary factors with CHDs. Our results found that the polymorphisms of UCP2 gene at rs659366 and rs660339, together with maternal dietary factors including excessive intake of pickled vegetables and smoked foods were associated with increased risks of CHDs in offspring. Regular intake of fresh meat, fish and shrimp, and milk products were associated with lower risks of CHDs in offspring. Besides, positive interaction between the dominant model of rs659366 and excessive intake of pickled vegetables was found in the additive interaction model (RERI = 1.19, P = 0.044). These findings provide the theoretical basis for gene screening and a new clue for the prevention of CHDs in offspring.
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http://dx.doi.org/10.1038/s41598-021-88057-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062668PMC
April 2021

Reclassification of as comb. nov. based on whole genome analysis.

Int J Syst Evol Microbiol 2021 Apr;71(4)

Department of Microbiome Science, Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, 72076 Tübingen, Germany.

The genera (family '') and (family ) are close relatives within the phylum . Members of these genera are strictly anaerobic, non-spore-forming and short straight rods with diverse phenotypes. Phylogenetic analysis of 16S rRNA genes suggest that splits into a polyphyletic clade. In an effort to ensure that family/genus names represent monophyletic clades, we performed a whole-genome based analysis of the genomes available for the cultured representatives of these genera: four species of and two strains of . A concatenated alignment of 135 shared protein sequences of single-copy core genes present in the included strains indicates that is indeed nested within the clade. Based on their evolutionary relationship, we propose the transfer of to the genus as comb. nov.
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http://dx.doi.org/10.1099/ijsem.0.004774DOI Listing
April 2021

Green, efficient extraction of bamboo hemicellulose using freeze-thaw assisted alkali treatment.

Bioresour Technol 2021 Apr 4;333:125107. Epub 2021 Apr 4.

Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, School of Light Industrial and Food Engineering, Guangxi University, Nanning 530004, PR China. Electronic address:

The premise of high value utilization of lignocellulosic biomass is effective separation of hemicellulose. In this paper, the extraction of bamboo hemicellulose using freeze-thaw assisted alkali treatment (FAT) was studied. The effect of alkali concentration, alkali treatment time, freezing temperature, and freeze-thaw time on the main components was studied. Bamboo was frozen at -30 °C for 12 h, thawed at room temperature, and then treated at 75 °C for 90 min with 7.0% alkali. The extraction rate of hemicellulose was as high as 64.71%. The purity of hemicellulose samples using conventional AT decreased from 82.63% to 78.56%. Hemicellulose with the same yield as that of conventional alkali treatment was obtained by further reducing the alkali concentration. The purity of hemicellulose samples increased from 82.63% to 89.45%. It had a higher purity, higher molecular weight, and lower polydispersity. A new, green and efficient alkaline extraction method for hemicellulose was developed.
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http://dx.doi.org/10.1016/j.biortech.2021.125107DOI Listing
April 2021

Absence of TNF Leads to Alternative Activation in Peritoneal Macrophages in Experimental Listeria Monocytogenes Infection.

Immunol Invest 2021 Apr 8:1-18. Epub 2021 Apr 8.

School of Life Sciences, Anhui Medical University, Hefei, China.

Macrophages are crucial effectors of innate immunity against the pathogenic bacterium . The pro-inflammatory cytokine tumour necrosis factor-α (TNF) has been shown to be crucial for resistance to and mice deficient in TNF signalling succumb quickly after infection. However, the mechanisms underlying TNF-mediated defence against infection have not been completely elucidated. Here, we demonstrate that TNF concurrently functions to support a pro-inflammatory M1 phenotype while actively blocking macrophage polarization to the M2 phenotype. Compared to WT mice, peritoneal macrophages in TNF-deficient mice inoculated with respond with M2 polarization by upregulating Arg1. Consistently, TNF blockade resulted in M2 polarization in peritoneal macrophages during infection. Additionally, TNF promotes the transition from M2 to M1 polarization in peritoneal macrophages. Further investigation of peritoneal macrophage polarization suggested the NF-κB pathway is involved in the TNF-dependent M2 to M1 shift. Conversely, treatment of peritoneal macrophage with a PPARγ agonist blunted the expression of M1 genes induced by TNF and reduced NF-κB signalling pathway activation. Competing signalling mechanisms therefore play an essential role in the ability of peritoneal macrophage to resolve infections with TNF playing an essential role in driving M1 polarization. LPM: large peritoneal macrophage; SPM: small peritoneal macrophage; LLO: listeriolysin O; iNOS: inducible nitric oxide synthase; DCs: dendritic cells.
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http://dx.doi.org/10.1080/08820139.2021.1902346DOI Listing
April 2021

CircRNA_OTUD7A upregulates FOXP1 expression to facilitate the progression of diffuse large B-cell lymphoma via acting as a sponge of miR-431-5p.

Genes Genomics 2021 Apr 8. Epub 2021 Apr 8.

Clinical Laboratory, The First Hospital of Yulin, No. 59 Yuxi Avenue, Yulin, 719000, Shaanxi, China.

Background: A growing number of studies have shown that circular RNA (circRNA) is an important regulator molecule in cancer progression, but it has been poorly studied in diffuse large b-cell lymphoma (DLBCL).

Objective: This study aimed to explore the role of circ_OTUD7A in DLBCL.

Methods: Relative expression levels of circ_OTUD7A, microRNA (miR)-431-5p and forkhead box P1 (FOXP1) were determined by quantitative real-time PCR (qRT-PCR). The proliferation of cells was elevated by colony formation assay and MTT assay. Western blot (WB) analysis was employed to measure the protein levels of proliferation marker, epithelial-mesenchymal transition (EMT) markers, cyclin marker, apoptosis markers and FOXP1. Moreover, the apoptosis, cell cycle process, migration and invasion of cells were detected using flow cytometry and transwell assay, respectively. In addition, the interaction between miR-431-5p and circ_OTUD7A or FOXP1 was confirmed by dual-luciferase reporter assay.

Results: Circ_OTUD7A was highly expressed in DLBCL, and its knockdown could inhibit DLBCL cell proliferation and metastasis, while promote cell cycle arrest and apoptosis. Similarly, FOXP1 also was upregulated in DLBCL, and its silencing could restrain the progression of DLBCL cells. Further experiments revealed that circ_OTUD7A could sponge miR-431-5p and miR-431-5p could target FOXP1. MiR-431-5p inhibitor could reverse the suppressive effect of circ_OTUD7A silencing on DLBCL progression, and FOXP1 overexpression also could reverse the inhibitory effect of miR-431-5p mimic on DLBCL progression.

Conclusion: Circ_OTUD7A promoted the progression of DLBCL by regulating the miR-431-5p/FOXP1 axis, which suggested that circ_OTUD7A might function as an oncogene in DLBCL.
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http://dx.doi.org/10.1007/s13258-021-01094-zDOI Listing
April 2021

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.

Aging (Albany NY) 2021 Apr 6;13(7):10703-10723. Epub 2021 Apr 6.

Department of Orthopedics and Spine Surgery, The First Affiliated Hospital, Anhui Medical University, Hefei 230032, Anhui, China.

Stem cell approaches have become an attractive therapeutic option for intervertebral disc degeneration (IVDD). Nucleus pulposus mesenchymal stem cells (NP-MSCs) participate in the regeneration and homeostasis of the intervertebral disc (IVD), but the molecular mechanisms governing these processes remain to be elucidated. Acid-sensing ion channels (ASICs) which act as key receptors for extracellular protons in central and peripheral neurons, have been implicated in IVDD where degeneration is associated with reduced microenvironmental pH. Here we show that ASIC1 and ASIC3, but not ASIC2 and ASIC4 are upregulated in human IVDs according to the degree of clinical degeneration. Subjecting IVD-derived NP-MSCs to pH 6.6 culture conditions to mimic pathological IVD changes resulted in decreased cell proliferation that was associated with cell cycle arrest and induction of senescence. Key molecular changes observed were increased expression of p53, p21, p27, p16 and Rb1. Instructively, premature senescence in NP-MSCs could be largely alleviated using ASIC inhibitors, suggesting both ASIC1 and ASIC3 act decisively upstream to activate senescence programming pathways including p53-p21/p27 and p16-Rb1 signaling. These results highlight the potential of ASIC inhibitors as a therapeutic approach for IVDD and broadly define an system that can be used to evaluate other IVDD therapies.
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http://dx.doi.org/10.18632/aging.202850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064223PMC
April 2021

Formation of filamentous fungal pellets in aerobic granular sludge via reducing temperature and dissolved oxygen: Characteristics of filamentous fungi and denitrification performance.

Bioresour Technol 2021 Jul 26;332:125056. Epub 2021 Mar 26.

School of Civil Engineering, Southeast University, Nanjing 210096, China. Electronic address:

A lab-scale sequencing batch reactor (SBR) using glucose as carbon source was operated for 500 days to investigate the formation of filamentous organisms and their function on stability of AGS system. After 250 days' stable operation under conditions of 25 ± 2 °C and dissolved oxygen (DO) of 4-5 mg/L (stage I), the temperature and DO were reduced to 10 ± 2 °C and DO of 1-2 mg/L until 280 days (stage II), to induce the growth of filamentous microorganisms. After that until 500 days (stage III), overgrowth of filamentous microorganisms with relative abundances of up to 19.46%, formation of black filamentous fungal pellets, and reconstruction of AGS granules were observed in turn. The relation between settling of AGS (SVI 30-72 mL/g) and filamentous microorganisms was revealed. Filamentous pellets were purified and identified as fungal Bradymyces and Knufia, with stronger denitrification performance on nitrite than nitrate. The results indicated that filamentous fungal pellets contributed to good sludge settling performance and promoted the denitrification process in AGS.
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http://dx.doi.org/10.1016/j.biortech.2021.125056DOI Listing
July 2021

Exceptional Mechanical Properties and Heat Resistance of Photocurable Bismaleimide Ink for 3D Printing.

Materials (Basel) 2021 Mar 30;14(7). Epub 2021 Mar 30.

College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou 362000, China.

Photosensitive resins used in three-dimensional (3D) printing are characterized by high forming precision and fast processing speed; however, they often possess poor mechanical properties and heat resistance. In this study, we report a photocurable bismaleimide ink with excellent comprehensive performance for stereolithography (SLA) 3D printing. First, the main chain of bismaleimide with an amino group (BDM) was synthesized, and then, the glycidyl methacrylate was grafted to the amino group to obtain the bismaleimide oligomer with an unsaturated double bond. The oligomers were combined with reaction diluents and photo-initiators to form photocurable inks that can be used for SLA 3D printing. The viscosity and curing behavior of the inks were studied, and the mechanical properties and heat resistance were tested. The tensile strength of 3D-printed samples based on BDM inks could reach 72.6 MPa (166% of that of commercial inks), glass transition temperature could reach 155 °C (205% of that of commercial inks), and energy storage modulus was 3625 MPa at 35 °C (327% of that of commercial inks). The maximum values of T, T, and T of the 3D samples printed by BDM inks reached 351.5, 449.6, and 451.9 °C, respectively. These photocured BDM inks can be used to produce complex structural components and models with excellent mechanical and thermal properties, such as car parts, building models, and pipes.
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http://dx.doi.org/10.3390/ma14071708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037760PMC
March 2021

Tumor acidic microenvironment-induced drug release of RGD peptide nanoparticles for cellular uptake and cancer therapy.

Colloids Surf B Biointerfaces 2021 Jun 4;202:111673. Epub 2021 Mar 4.

School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, PR China. Electronic address:

Spatial accuracy is crucial in drug delivery, especially to increase the efficacy and reduce the side effects of antitumor drugs. In this study, we developed a simple and broadly applicable strategy in which a target peptide ligand was introduced to construct a pH-responsive drug-loading system to achieve targeted delivery and drug release in lesions. In addition to reaching the tumor tissue through passive targeting modalities such as the enhanced permeability and retention (EPR) effect, active targeting nanoparticles used RGD motifs coupled to nanocarriers to specifically bind certain integrins, such as ανβ3, which is expressed on the surface of tumor cells, to achieve active tumor cell targeting. Self-assembling peptides have significant advantages in their structural design. The amphiphilic peptide LKR could form a spherical and self-assembled nanoparticle, which encapsulated the fat-soluble antitumor drug doxorubicin (Dox) in neutral medium. The Dox-encapsulating peptide nanoparticles swelled and burst, rapidly releasing Dox in an acidic microenvironment. Flow cytometry and fluorescence detection showed that the self-assembled LKR nanoparticles enhanced the drug accumulation in tumor cells compared with normal mammalian cells. The Dox-encapsulating peptide nanoparticles exhibited desirable antitumor effects in vivo. In summary, the acidic microenvironment of tumors was used to induce drug release from a targeted peptide drug-loading system to enhance cellular uptake and therapeutic effects in situ, providing a promising therapeutic approach for the treatment of major diseases such as hepatoma.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111673DOI Listing
June 2021

Clinical manifestation and phenotypic analysis of novel gene mutation in 28 Chinese children with hereditary spherocytosis.

Mol Genet Genomic Med 2021 Feb 23:e1577. Epub 2021 Feb 23.

Department of Pediatrics, Changhai Hospital, Naval Military Medical University, Shanghai, China.

Purpose: Objective to summarize the clinical features and laboratory findings of 28 Chinese children with hereditary spherocytosis (HS), and analyze these mutations.

Method: Collected and analyzed the clinical data of all children and their parents, and completed the relevant laboratory examinations of all children. Analyzed the sequence of related genes by second-generation sequencing technology, and verified the suspected mutations by Sanger sequencing method. Analyzed all biological information using the Single Nucleotide Polymorphism database, the 1000 Human Genome Project, and the Exosome Aggregation Consortium.

Result: New mutations were detected in the HS coding region of 28 children. Among them, there were 13 cases (46.4%) with ANK1 mutation, 10 cases (35.7%) with SPTB mutation, three cases (10.7%) with SLC4A1 mutation, and two cases (7.2%) with SPTA1 mutation. All mutations cause amino acid changes in the coding gene, as well as subsequent changes in protein structure or loss of function.

Conclusion: All the newly discovered gene coding region mutation sites detected are the suspected pathogenic causes of the 28 Chinese children. At the same time, the second-generation gene sequencing technology is an effective means to diagnose HS. Different mutation types and different mutation regions have no significant correlation with the severity of anemia. The novel gene mutation sites in 28 children studied in this paper have not yet been included in the human genome database, dbSNP (v138), or ExAC database. The new gene mutations found in HS children can provide a theoretical basis for further exploring the genetic causes of HS in Chinese children.
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http://dx.doi.org/10.1002/mgg3.1577DOI Listing
February 2021

Transcriptomic and Functional Analyses Indicate Novel Anti-viral Mode of Actions on Tobacco Mosaic Virus of a Microbial Natural Product ε-Poly-l-lysine.

J Agric Food Chem 2021 Feb 15;69(7):2076-2086. Epub 2021 Feb 15.

College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.

Novel anti-viral natural product ε-poly-l-lysine (ε-PL) produced by is a homopolymer of l-lysine, of which the underlying molecular mode of action remains to be further elucidated. In this study, ε-PL induced significant fragmentation of tobacco mosaic virus (TMV) virions and delayed the systemic infection of TMV-GFP as well as wild-type TMV in plants. ε-PL treatment also markedly inhibited RNA accumulation of TMV in tobacco BY-2 protoplasts. The results of RNA-seq indicated that the agent induced significantly differential expression of genes that are associated with defense response, stress response, autophagy, and ubiquitination. Among them, 15 critical differential expressed genes were selected for real-time quantitative PCR validation. We further demonstrated that ε-PL can induce host defense responses by assessing the activity of several defense-related enzymes in plants. Our results provided valuable insights into molecular anti-viral mode of action for ε-PL, which is expected to be applied as a novel microbial natural product against plant virus diseases.
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http://dx.doi.org/10.1021/acs.jafc.0c07357DOI Listing
February 2021

Building on recent cytomorphologic descriptions of NTRK-rearranged thyroid carcinoma.

Cancer Cytopathol 2021 Feb 9. Epub 2021 Feb 9.

Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire.

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http://dx.doi.org/10.1002/cncy.22413DOI Listing
February 2021

Recurrent non-severe hypoglycemia aggravates cognitive decline in diabetes and induces mitochondrial dysfunction in cultured astrocytes.

Mol Cell Endocrinol 2021 04 2;526:111192. Epub 2021 Feb 2.

Department of Endocrinology, Fujian Medical University Union Hospital, Fuzhou, 350001, China. Electronic address:

The present study aimed to determine the relationship between astrocytes and recurrent non-severe hypoglycemia (RH) -associated cognitive decline in diabetes. RH induced cognitive impairment and neuronal cell death in the cerebral cortex of diabetic mice, accompanied by excessive activation of astrocytes. Levels of the neurotrophins BDNF and GDNF, together with BDNF and GDNF- related signaling, were downregulated by RH. In vitro, recurrent low glucose (RLG) impaired cell viability and induced apoptosis of high-glucose cultured astrocytes. Accumulating mitochondrial ROS and dysregulated mitochondrial functions, including abnormal morphology, decreased membrane potential, downregulated ATP levels, and disrupted bioenergetic status, were observed in these cells. SS-31 mediated protection of mitochondrial functions reversed RLG-induced cell viability defects and neurotrophin production. These findings demonstrate that RH induced astrocyte overactivation and mitochondrial dysfunction, leading to astrocyte-derived neurotrophin disturbance, which might contribute to diabetic cognitive decline. Targeting astrocyte mitochondria might represent a neuroprotective therapy for hypoglycemia-associated neurodegeneration in diabetes.
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http://dx.doi.org/10.1016/j.mce.2021.111192DOI Listing
April 2021

RNA-Seq analysis reveals the growth and photosynthetic responses of rapeseed (Brassica napus L.) under red and blue LEDs with supplemental yellow, green, or white light.

Hortic Res 2020 Dec 1;7(1):206. Epub 2020 Dec 1.

College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, China.

Compound light is required for plant growth and development, but the response mechanisms of plants are undercharacterized and not fully understood. The present study was undertaken to evaluate the effect of supplemental light (green light, G; white light, W; yellow light, Y) added to red-blue light (RB) and sole W on the growth and photosynthesis of rapeseed seedlings. The results revealed that supplemental G/W improved the growth and photosynthesis of seedlings, but supplemental Y significantly reduced the photosynthetic rate and palisade tissue layer. Sole W caused similar responses in terms of growth, leaf development, oxidative damage, and antioxidant capability as supplemental Y. In total, 449, 367, 813, and 751 differentially expressed genes (DEGs) were identified under supplemental G, Y, and W and sole W, respectively, compared to RB. The DEGs under different lights were closely associated with pathways such as light stimulus and high-light response, root growth, leaf development, photosynthesis, photosynthesis-antenna proteins, carbohydrate synthesis and degradation, secondary metabolism, plant hormones, and antioxidant capacity, which contributed to the distinct growth and photosynthesis under different treatments. Our results suggest that Y is more likely substituted by other wavelengths to achieve certain effects similar to those of supplemental Y, while G has a more distinctive effect on rapeseed. Taken together, supplementation RB with G/W promotes the growth of rapeseed seedlings in a controlled environment.
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http://dx.doi.org/10.1038/s41438-020-00429-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705657PMC
December 2020

Typical reactive carbonyl compounds in food products: Formation, influence on food quality, and detection methods.

Compr Rev Food Sci Food Saf 2020 03 11;19(2):503-529. Epub 2020 Feb 11.

NHC Key Laboratory of Food Safety Risk Assessment, Food Safety Research Unit (2019RU014) of Chinese Academy of Medical Science, China National Center for Food Safety Risk Assessment, Beijing, China.

Reactive carbonyl compounds are a large group of highly reactive electrophilic compounds containing one or more carbonyl groups, which can be created by lipid oxidation both in vivo and in food. Malondialdehyde (MDA) and 4-hydroxy-2-nonenel (HNE) are the two most important reactive carbonyl compounds in food. They can react with proteins and nucleic acids and cause biological damage to cells and lead to carbonyl stress. Therefore, they are regarded as representative products of lipid oxidation, toxic molecules, and biomarkers of oxidative stress. Apart from biological toxicity, they can also react with myoglobin and myofibrillar protein and further affect color, gel properties, hydrophobicity, or other properties of food. However, the effects of MDA and HNE on food qualities have not received as much attentions and it is noteworthy that the existing analytical methods for detecting MDA and HNE have a variety of limitations due to the complexity of food samples. To provide a comprehensive understanding of HNE and MDA, the formation mechanism, occurrence, and analytical methods for MDA and HNE in food matrix were summarized in this article. Emphasis is focused on formation mechanism including non-enzymatic pathway and enzymatic pathway, and detection methods including the extraction methods, the new development of sample pre-treatment technology and the selection of derivative reagents. Impressively, the reaction mechanism of MDA and HNE with myoglobin or myofibrillar protein is also described to explain how MDA and HNE affect food quality.
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http://dx.doi.org/10.1111/1541-4337.12535DOI Listing
March 2020

Heterojunction interface of zinc oxide and zinc sulfide promoting reactive molecules activation and carrier separation toward efficient photocatalysis.

J Colloid Interface Sci 2021 Apr 2;588:826-837. Epub 2020 Dec 2.

State Key Laboratory of Mechanical Transmissions, College of Materials Science and Engineering, Chongqing University, Chongqing 400044, China. Electronic address:

Heterojunction photocatalysts, which can alleviate the low carrier separation efficiency and insufficient light absorption capacity of a single catalyst, have received widespread attention. However, the specific interfacial structure of the heterojunction and its effect on the photocatalytic reaction is still unclear. Herein, a battery of zinc oxide/zinc sulfide (ZnO@ZnS) heterojunction microspheres with different degrees of sulfuration were successfully constructed via a facile hydrothermal method. The as-prepared photocatalysts shown decent aerobic nitric oxide (NO) oxidation performance under visible light irradiation, and the results of various characterization techniques illustrated that the superior photoactivity could be ascribed to the spatial separation of photoinduced electron-hole pairs due to the synergy of the internal electric field and the band offset. More importantly, density functional theory (DFT) calculations revealed that the heterojunction interface can significantly promote the generation of reactive oxygen species (ROS) and NO reaction intermediates and thus accelerate the photocatalytic reaction. Finally, in situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) technology was used to time-dependently monitor the NO oxidation process, revealing the photocatalytic mechanism. This work investigated the role of the heterojunction interface in the gas-phase catalytic reaction, broadening the practical application of the ZnO@ZnS heterojunction.
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http://dx.doi.org/10.1016/j.jcis.2020.11.118DOI Listing
April 2021

A large-scale internal validation study of unsupervised virtual trichrome staining technologies on nonalcoholic steatohepatitis liver biopsies.

Mod Pathol 2021 04 9;34(4):808-822. Epub 2020 Dec 9.

Emerging Diagnostic and Investigative Technologies, Clinical Genomics and Advanced Technologies, Department of Pathology and Laboratory Medicine, Dartmouth Hitchcock Medical Center, Lebanon, NH, 03756, USA.

Non-alcoholic steatohepatitis (NASH) is a fatty liver disease characterized by accumulation of fat in hepatocytes with concurrent inflammation and is associated with morbidity, cirrhosis and liver failure. After extraction of a liver core biopsy, tissue sections are stained with hematoxylin and eosin (H&E) to grade NASH activity, and stained with trichrome to stage fibrosis. Methods to computationally transform one stain into another on digital whole slide images (WSI) can lessen the need for additional physical staining besides H&E, reducing personnel, equipment, and time costs. Generative adversarial networks (GAN) have shown promise for virtual staining of tissue. We conducted a large-scale validation study of the viability of GANs for H&E to trichrome conversion on WSI (n = 574). Pathologists were largely unable to distinguish real images from virtual/synthetic images given a set of twelve Turing Tests. We report high correlation between staging of real and virtual stains ([Formula: see text]; 95% CI: 0.84-0.88). Stages assigned to both virtual and real stains correlated similarly with a number of clinical biomarkers and progression to End Stage Liver Disease (Hazard Ratio HR = 2.06, 95% CI: 1.36-3.12, p < 0.001 for real stains; HR = 2.02, 95% CI: 1.40-2.92, p < 0.001 for virtual stains). Our results demonstrate that virtual trichrome technologies may offer a software solution that can be employed in the clinical setting as a diagnostic decision aid.
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http://dx.doi.org/10.1038/s41379-020-00718-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985027PMC
April 2021

Effects of dendrobium polysaccharides on human brain microvascular endothelial cell injury induced by ox-LDL via regulating the miR-378 expression.

Cell Mol Biol (Noisy-le-grand) 2020 Oct 31;66(7):66-71. Epub 2020 Oct 31.

Neurology Department, Shanghai TCM-Integrated Hospital Affiliated to Shanghai University of TCM, Shanghai, 200082, China.

Brain microvascular endothelial cells are a key part of the blood-brain barrier. This experiment was set up to investigate the effect and molecular mechanism of Dendrobium polysaccharide on oxidized low-density lipoprotein (ox-LDL)-induced damage to the human brain microvascular endothelial cells. For this purpose, human brain microvascular endothelial cells HBMEC were divided into control group (without any treatment), ox-LDL group (50 μg/mL ox-LDL), Dendrobium polysaccharide low, medium and high concentration group (0.1 μg/L, 0.2 μg/L, 0.4 μg/L Dendrobium polysaccharide+50 μg/mL ox-LDL), ox-LDL+miR-NC group (transfection miR-378 mimic negative control+50 μg/mL ox-LDL), ox-LDL+miR-378 group (transfected miR-378 mimics+50 μg/mL ox-LDL), ox-LDL+DP+anti-miR-NC group (transfected miR-378 inhibitor negative control +0.4 μg/L Dendrobium polysaccharide+50 μg/mL ox-LDL), ox-LDL+DP+anti-miR-378 group (transfected miR-378 inhibitor+0.4 μg/L Dendrobium polysaccharide+50 μg/mL ox-LDL ). The kit was used to detect the levels of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and catalase (CAT); flow cytometry to detect apoptosis; and Western blot to detect B-cell lymph tumor/leukemia-2 (Bcl-2) and Bcl-2 related X (Bax) protein expression; real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the expression of miR-378. Results showed that after treatment with different concentrations of Dendrobium polysaccharides, MDA levels were decreased in ox-LDL-induced human brain microvascular endothelial cells, SOD and CAT activities were increased, apoptosis rate was decreased, Bcl-2 expression was increased, Bax expression was decreased, miR-378 expression was increased, in a dose-dependent manner (P<0.05). Overexpression of miR-378 inhibits ox-LDL-induced oxidative stress and apoptosis in human brain microvascular endothelial cells. Inhibition of miR-378 expression reversed the effect of Dendrobium polysaccharide on ox-LDL-induced damage to human brain microvascular endothelial cells. Then dendrobium polysaccharide may inhibit ox-LDL-induced oxidative stress and apoptosis in human brain microvascular endothelial cells by up-regulating the expression of miR-378.
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October 2020

Reduction of depression symptoms in laryngeal cancer patients receiving psychology services.

Am J Transl Res 2020 15;12(10):6637-6645. Epub 2020 Oct 15.

Department of Otorhinolaryngology, Weifang People's Hospital No. 151 Guangwen Street, Kuiwen District, Weifang 261041, Shandong, China.

Depression is prevalence in patients with laryngeal cancer. In this study, we aim to investigate whether psychological intervention could reduce the depression of patients receiving cancer treatment. In this study, 258 patients with laryngeal cancer were assigned into two groups with or without psychological intervention. The depression symptoms of all patients were assessed using Hamilton depression rating scale (HAMD) and Hamilton anxiety rating scale (HAMA). Europe Organization for research and Treatment of cancer Quality of life Questionnaire (EORTC QLQ-C30) was used to measure the life quality of the participants. Other measurements including general attitudes towards psychology services, hospitalization duration and expenses were analyzed. We found that patients with intervention showed more positive attitudes towards psychology services compared to those in control group. Hospitalization duration and expenses were significantly less in intervention group compared to control group. In addition, the intervention group showed a significantly lower HAMD and HAMA scores and improved life quality than control group. Patients aged lower than 40 years showed more obvious reductions in HAMD and HAMA scores and better life quality compared to older patients. In conclusion, our study demonstrated that psychological intervention was necessary and effective in patients with laryngeal cancer during treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653580PMC
October 2020

Characterization and anti-inflammation of a polysaccharide produced by Chaetomium globosum CGMCC 6882 on LPS-induced RAW 264.7 cells.

Carbohydr Polym 2021 Jan 22;251:117129. Epub 2020 Sep 22.

College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China. Electronic address:

In this study, a polysaccharide (CGP-BG) was successfully produced from waste distillers' grain by Chaetomium globosum CGMCC 6882. Chemical analysis demonstrated that CGP-BG contained 3.49 ± 0.51 % protein. The molecular weight of CGP-BG was 52.37 KDa, which was composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid, and glucuronic acid in the molar ratio of 4.11:7.34:13.31:20.99:1.07:0.91:4.75:0.36. In vitro assay showed that CGP-BG had scavenging activities on hydroxyl radical, DPPH radical, superoxide anion, and ABTS radical in a dose-dependent manner. Meanwhile, CGP-BG significantly inhibited nitric oxide and pro-inflammatory cytokine (TNF-α, IL-6, and IL-1β) production in the lipopolysaccharide (LPS)-induced RAW 264.7 cells. Moreover, CGP-BG notably improved the antioxidant status of LPS-treated macrophages by enhancing superoxide dismutase, glutathione peroxidase, and antioxidant capacity and mitigating malondialdehyde. This work provided the basis for the application of CGP-BG as an anti-inflammatory and antioxidant agent.
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http://dx.doi.org/10.1016/j.carbpol.2020.117129DOI Listing
January 2021

Targeted miR-21 loaded liposomes for acute myocardial infarction.

J Mater Chem B 2020 12 28;8(45):10384-10391. Epub 2020 Oct 28.

School of Chemistry and Chemical Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150001, China.

Acute and persistent myocardial ischemia is the main cause of acute myocardial infarction (AMI) and heart failure. MicroRNA-21(miR-21) contributes to the pathophysiological consequences of acute myocardial infarction by targeting downstream crucial regulators. Thus, miR-21 mimics are a promising strategy for the treatment of AMI. However, their poor stability and insufficient cellular uptake are the major challenges. Herein, we encapsulated miR-21 mimics into liposomes modified with the cardiac troponin T (cTnT) antibody for targeted delivery of miR-21(cT-21-LIPs) to the ischemic myocardium. The cT-21-LIPs exhibited enhanced targeting efficiency to hypoxia primary cardiomyocytes in vitro and improved accumulation in the ischemic heart of AMI rats after injection via the tail vein due to the specifical target to overexpressed troponin. The cT-21-LIPs could significantly improve the cardiac function and decrease the infarct size after AMI, while maintaining the viability of cardiomyocytes. This design provides a novel strategy for delivering small nucleotide drugs specifically to the infarcted heart, which may find great potential in clinics.
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http://dx.doi.org/10.1039/d0tb01821jDOI Listing
December 2020

Synthesis of High-Purity SiC Nanowires via Catalyst-Free Pyrolysis of SiO/Si and Sponge-Like Graphene Oxide.

ACS Omega 2020 Oct 23;5(39):25319-25325. Epub 2020 Sep 23.

Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350000, People's Republic of China.

The large-scale synthesis of high-purity SiC nanowires is a challenge. In this context, sponge-like graphene oxide (GO) was used as a carbon source as well as a reaction template for directly synthesizing SiC nanowires. GO was completely reacted with SiO to prepare high-purity 3C-SiC nanowires by thermal evaporation and carbothermal reduction without the use of any catalyst, rather than by epitaxy. Characterization was conducted using X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and selected-area electron diffraction. The SiC nanowires had lengths of several tens of micrometers and a perfect single-crystalline structure with a bamboo-like morphology.
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http://dx.doi.org/10.1021/acsomega.0c03619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542835PMC
October 2020

Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition.

J Lipid Res 2020 12 2;61(12):1629-1644. Epub 2020 Oct 2.

Department of Internal Medicine, Virginia Commonwealth University, Richmond, VA, USA; Department of Veterans Affairs, McGuire Veterans Administration Medical Center, Richmond, VA, USA; Massey Cancer Center, Virginia Commonwealth University, Richmond, VA, USA.

NAFLD is an important public health issue closely associated with the pervasive epidemics of diabetes and obesity. Yet, despite NAFLD being among the most common of chronic liver diseases, the biological factors responsible for its transition from benign nonalcoholic fatty liver (NAFL) to NASH remain unclear. This lack of knowledge leads to a decreased ability to find relevant animal models, predict disease progression, or develop clinical treatments. In the current study, we used multiple mouse models of NAFLD, human correlation data, and selective gene overexpression of steroidogenic acute regulatory protein (StarD1) in mice to elucidate a plausible mechanistic pathway for promoting the transition from NAFL to NASH. We show that oxysterol 7α-hydroxylase (CYP7B1) controls the levels of intracellular regulatory oxysterols generated by the "acidic/alternative" pathway of cholesterol metabolism. Specifically, we report data showing that an inability to upregulate CYP7B1, in the setting of insulin resistance, results in the accumulation of toxic intracellular cholesterol metabolites that promote inflammation and hepatocyte injury. This metabolic pathway, initiated and exacerbated by insulin resistance, offers insight into approaches for the treatment of NAFLD.
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http://dx.doi.org/10.1194/jlr.RA120000924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707165PMC
December 2020

Fine Needle Aspiration Cytology of Malignant Digestive System Gastrointestinal Neuroectodermal Tumor in a Lymph Node Metastasis from a Previously Diagnosed Liver Primary: A Case Report and Review of Literature.

Diagn Cytopathol 2021 Mar 25;49(3):E130-E136. Epub 2020 Sep 25.

Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA.

Malignant gastrointestinal neuroectodermal tumor (GNET) is an extremely rare neoplasm. Immunohistochemically, GNET typically demonstrates neural differentiation but lacks melanocytic differentiation, making it distinct from clear cell sarcoma of the soft tissues (CCS). Herein we report for the first time the cytomorphologic features of lymph node metastasis from presumably liver GNET. A 36-year-old female presented with fevers, night sweats, loss of appetite, and a 20-lbs weight loss. Radiographic imaging showed a 13 cm heterogeneously enhancing mass in the right lobe of the liver and a hypermetabolic 0.9 cm periportal lymph node on positron emission tomography-computed tomography (PET/CT). Initially, a CT-guided liver biopsy was performed followed by right hepatic lobectomy and portal lymphadenectomy. The liver biopsy and resection showed an S100-protein and SOX10 positive malignant neoplasm and genomic profiling of liver biopsy revealed EWSR1-CREB1gene rearrangement. These findings in conjunction with the morphologic and immunohistochemical profile were diagnostic of GNET. Two months later, she presented with recurrent lymphadenopathy in the upper abdomen. Fine needle aspiration of the periportal nodal mass revealed single and clusters of primitive, large to medium-sized neoplastic cells with round to oval nuclei, high nuclear-cytoplasmic ratio, vesicular chromatin, and prominent nucleoli. The tumor cells were S100 protein and SOX10 positive, consistent with metastasis of the patient's recently diagnosed malignant digestive system GNET. Palliative chemotherapy was administered but the patient died a few days later, 4 months from the initial diagnosis. Awareness of this entity and judicial use of ancillary studies including molecular testing are essential for achieving accurate diagnosis.
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http://dx.doi.org/10.1002/dc.24624DOI Listing
March 2021

Influences of galactose ligand on the uptake of TADF liposomes by HepG cells.

Photodiagnosis Photodyn Ther 2020 Dec 18;32:102014. Epub 2020 Sep 18.

Daqing Campus of Harbin Medical University, 1 Xinyang Rd Daqing, 163319, China. Electronic address:

Glucose is the main energy substance to drive the physiological events of the cell.. Malignant cells exhibit a much higher rate of glycolysis than healthy cells to relieve the increased needs of energy. The higher metabolic rate induces the over-expression of the Glucose Transporter (GLUT) to transport more glucose into malignant cells. Our research regarded overexpressive GLUT as a target of nanoparticles. Substrate of GLUT galactose conjugated Polyethylene glycol-Distearyl phosphatidyl ethanolamine (PEG-DSPE) as a kind of ligand was selected to modified liposome. Thermally activated delayed fluorescence (TADF) was encapsulated as fluorescent probe to evaluate its abilities of targeting malignant cells, and the results of confocal microscopy and flow cytometry demonstrated that Galactose-PEG-DSPE modified liposome had the stronger efficiency of cellular uptake by HepG2 cells compared with Blank-PEG-DSPE modified liposome. The effect of GLUT1 inhibitor on cellular uptake of Galactose-PEG-DSPE modified liposomes showed that the mechanism might be relative to Warburg effect causing GLUT overexpression.
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http://dx.doi.org/10.1016/j.pdpdt.2020.102014DOI Listing
December 2020

Osteocalcin and measures of adiposity: a systematic review and meta-analysis of observational studies.

Arch Osteoporos 2020 09 18;15(1):145. Epub 2020 Sep 18.

Department of Physiology, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.

Osteocalcin, the osteoblast-derived protein, has been shown to be modulated in patients with problematic glucose metabolism. Our systematic review and meta-analysis found that in humans, higher blood osteocalcin level is associated with lower body indices of fat.

Purpose/introduction: Osteocalcin (OC) was found to be inversely correlated with measures of glucose and energy metabolism, with some groups suggesting the undercarboxylated form (ucOC) to be metabolically active, although the link is not clear, especially in humans. Given obesity is a major risk factor for metabolic disorders, we aimed to assess the correlation between OC and two measures of body weight: body mass index (BMI) and percentage body fat (%BF).

Methods: MEDLINE and EMBASE were searched to identify observational studies in adult populations that reported the crude correlation coefficients (r) between OC and BMI and %BF. Pool r were obtained using random-effects models.

Results: Fifty-one publications were included in this analysis. Both total OC (TOC) (pooled r = - 0.151, 95% CI - 0.17, - 0.130; I = 52%) and ucOC (pooled r = - 0.060, 95% CI - 0.103, - 0.016; I = 54%) were inversely correlated with BMI. The pooled r between TOC and BMI in Caucasian-and-other-regions (r = - 0.187) were stronger than those in Asian populations (r = - 0.126; intra-group p = 0.002; R = 0.21). The mean/median BMI of the reported cohort was the major contributor to between-study heterogeneity in correlation between TOC/ucOC and BMI (R = 0.28 and 0.77, respectively). Both TOC and ucOC were also inversely correlated with %BF (TOC: pooled r = - 0.185, 95% CI - 0.257 to - 0.112; ucOC: pooled r = - 0.181, 95% CI - 0.258 to - 0.101).

Conclusion: Higher OC and ucOC were correlated with lower BMI and %BF. The inverse correlations between TOC/ucOC and BMI appear to be affected by ethnicity and obesity status.
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http://dx.doi.org/10.1007/s11657-020-00812-6DOI Listing
September 2020

Insights into the Oxidative Stress Response of serovar Enteritidis Revealed by the Next Generation Sequencing Approach.

Antioxidants (Basel) 2020 Sep 10;9(9). Epub 2020 Sep 10.

Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN 55108, USA.

As a facultative intracellular pathogen, Enteritidis must develop an effective oxidative stress response to survive exposure to reactive oxygen species within the host. To study this defense mechanism, we carried out a series of oxidative stress assays in parallel with a comparative transcriptome analyses using a next generation sequencing approach. It was shown that the expression of 45% of the genome was significantly altered upon exposure to HO. Quantitatively the most significant (≥100 fold) gene expression alterations were observed among genes encoding the sulfur utilization factor of Fe-S cluster formation and iron homeostasis. Our data point out the multifaceted nature of the oxidative stress response. It includes not only numerous mechanisms of DNA and protein repair and redox homeostasis, but also the key genes associated with osmotic stress, multidrug efflux, stringent stress, decrease influx of small molecules, manganese and phosphate starvation stress responses. Importantly, this study revealed that oxidatively stressed Enteritidis cells simultaneously repressed key motility encoding genes and induced a wide range of adhesin- and salmonellae-essential virulence-encoding genes, that are critical for the biofilm formation and intracellular survival, respectively. This finding indicates a potential intrinsic link between oxidative stress and pathogenicity in non-typhoidal that needs to be empirically evaluated.
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http://dx.doi.org/10.3390/antiox9090849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555449PMC
September 2020

Metastatic low-grade endometrial stromal sarcoma after 24 years: A case report and review of recent molecular genetics.

Diagn Cytopathol 2021 Mar 10;49(3):E99-E105. Epub 2020 Sep 10.

Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center and Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA.

Low-grade endometrial stromal sarcoma (LGESS) is a rare malignant uterine tumor with the potential to metastasize years after the primary resection. There is a broad differential diagnosis for endometrial stromal sarcomas (ESS), including both benign and malignant entities. Herein, we present the case of a 64-year-old female with metastatic LGESS to the lung, diagnosed by cytology, 24 years after her initial presentation. This report discusses the cytomorphologic and histopathologic characteristics, and ancillary studies including immunohistochemical stains and recent advances in molecular diagnostics of ESS. Accurate diagnosis of spindle cell lesions in the lung can be challenging. As such, this case highlights the instrumental role of ancillary testing and molecular diagnostics to achieve a more definitive diagnosis.
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http://dx.doi.org/10.1002/dc.24601DOI Listing
March 2021