Publications by authors named "Xiaoyan Zhang"

1,090 Publications

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Rare Case of Syringotropic Mycosis Fungoides Presenting as Poikiloderma-Like Erythroderma Concurrent With Intermittent Fever and a Literature Review.

Am J Dermatopathol 2021 Jul 20. Epub 2021 Jul 20.

Department of Dermatovenereology, West China Hospital, Sichuan University, Sichuan, China.

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http://dx.doi.org/10.1097/DAD.0000000000002031DOI Listing
July 2021

Single-Molecule Real-Time and Illumina-Based RNA Sequencing Data Identified Vernalization-Responsive Candidate Genes in Faba Bean ( L.).

Front Genet 2021 5;12:656137. Epub 2021 Jul 5.

Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

Faba bean ( L.) is one of the most widely grown cool season legume crops in the world. Winter faba bean normally has a vernalization requirement, which promotes an earlier flowering and pod setting than unvernalized plants. However, the molecular mechanisms of vernalization in faba bean are largely unknown. Discovering vernalization-related candidate genes is of great importance for faba bean breeding. In this study, the whole transcriptome of faba bean buds was profiled by using next-generation sequencing (NGS) and single-molecule, real-time (SMRT) full-length transcriptome sequencing technology. A total of 29,203 high-quality non-redundant transcripts, 21,098 complete coding sequences (CDS), 1,045 long non-coding RNAs (lncRNAs), and 12,939 simple sequence repeats (SSRs) were identified. Furthermore, 4,044 differentially expressed genes (DEGs) were identified through pairwise comparisons. By Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, these differentially expressed transcripts were found to be enriched in binding and transcription factor activity, electron carrier activity, rhythmic process, and receptor activity. Finally, 50 putative vernalization-related genes that played important roles in the vernalization of faba bean were identified; we also found that the levels of vernalization-responsive transcripts showed significantly higher expression levels in cold-treated buds. The expression of , one of the candidate genes, was sensitive to vernalization. Ectopic expression of in brought earlier flowering. In conclusion, the abundant vernalization-related transcripts identified in this study will provide a basis for future researches on the vernalization and faba bean breeding and established a reference full-length transcriptome for future studies on faba bean.
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http://dx.doi.org/10.3389/fgene.2021.656137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287337PMC
July 2021

FUBP1 promotes colorectal cancer stemness and metastasis via DVL1-mediated activation of Wnt/β-catenin signaling.

Mol Oncol 2021 Jul 20. Epub 2021 Jul 20.

Program of Molecular Medicine, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.

Distant metastasis is, unfortunately, the leading cause of death in colorectal cancer (CRC). Approximately 50% of CRC patients develop liver metastases, while 10-30% of patients develop pulmonary metastases. The occurrence of metastasis is considered to be almost exclusively driven by cancer stem cells (CSCs) formation. However, the key molecules that confer the transformation to stem cells in CRC, and subsequent metastasis, remain unclear. Far upstream element-binding protein 1 (FUBP1), a transcriptional regulator of c-Myc, was screened in CSCs of CRC by mass spectrometry and was examined by immunohistochemistry in a cohort of CRC tissues. FUBP1 was upregulated in 85% of KRAS-mutant and 25% of wild-type CRC patients. Further, whether in KRAS-mutant or wild-type patients, elevated FUBP1 was positively correlated with CRC lymph node metastasis and clinical stage, and negatively associated with overall survival. Overexpression of FUBP1 significantly enhanced CRC cell migration, invasion, tumor sphere formation, and CD133 and ALDH1 expression in vitro, and tumorigenicity in vivo. Mechanistically, FUBP1 promoted the initiation of CSCs by activating Wnt/β-catenin signaling via directly binding to the promoter of DVL1, a potent activator of β-catenin. Knockdown of DVL1 significantly inhibited the transformation to stem cells in, as well as the tumorigenicity of, CRC. Activation of Wnt/β-catenin signaling by DVL1 increased pluripotent transcription factors, including c-Myc, NANOG, SOX2. Moreover, FUBP1 was upregulated at the post-transcriptional level. Elevated FUBP1 levels in KRAS wild-type CRC patients is due to the decrease of Smurf2, which promotes ubiquitin-mediated degradation of FUBP1. In contrast, FUBP1 was upregulated in KRAS-mutant patients through both inhibition of caspase-3-dependent cleavage and decreased Smurf2. Our results demonstrate, for the first time, that FUBP1 is an oncogene, initiating the development of CSCs, as well as a new powerful endogenous Wnt-signaling agonist that could provide an important prognostic factor and therapeutic target for metastasis in both KRAS-mutant and wild-type CRC.
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http://dx.doi.org/10.1002/1878-0261.13064DOI Listing
July 2021

Establishment of an Ultrasound Malignancy Risk Stratification Model for Thyroid Nodules Larger Than 4 cm.

Front Oncol 2021 29;11:592927. Epub 2021 Jun 29.

Department of Ultrasound, China-Japan Friendship Hospital, Beijing, China.

Background: The incidence and mortality of thyroid cancer, including thyroid nodules > 4 cm, have been increasing in recent years. The current evaluation methods are based mostly on studies of patients with thyroid nodules < 4 cm. The aim of the current study was to establish a risk stratification model to predict risk of malignancy in thyroid nodules > 4 cm.

Methods: A total of 279 thyroid nodules > 4 cm in 267 patients were retrospectively analyzed. Nodules were randomly assigned to a training dataset (n = 140) and a validation dataset (n = 139). Multivariable logistic regression analysis was applied to establish a nomogram. The risk stratification of thyroid nodules > 4 cm was established according to the nomogram. The diagnostic performance of the model was evaluated and compared with the American College Radiology Thyroid Imaging Reporting and Data System (ACR TI-RADS), Kwak TI-RADS and 2015 ATA guidelines using the area under the receiver operating characteristic curve (AUC).

Results: The analysis included 279 nodules (267 patients, 50.6 ± 13.2 years): 229 were benign and 50 were malignant. Multivariate regression revealed microcalcification, solid mass, ill-defined border and hypoechogenicity as independent risk factors. Based on the four factors, a risk stratified clinical model was developed for evaluating nodules > 4 cm, which includes three categories: high risk (risk value = 0.8-0.9, with more than 3 factors), intermediate risk (risk value = 0.3-0.7, with 2 factors or microcalcification) and low risk (risk value = 0.1-0.2, with 1 factor except microcalcification). In the validation dataset, the malignancy rate of thyroid nodules > 4 cm that were classified as high risk was 88.9%; as intermediate risk, 35.7%; and as low risk, 6.9%. The new model showed greater AUC than ACR TI-RADS (0.897 0.855, p = 0.040), but similar sensitivity (61.9% 57.1%, p = 0.480) and specificity (91.5% 93.2%, p = 0.680).

Conclusion: Microcalcification, solid mass, ill-defined border and hypoechogenicity on ultrasound may be signs of malignancy in thyroid nodules > 4 cm. A risk stratification model for nodules > 4 cm may show better diagnostic performance than ACR TI-RADS, which may lead to better preoperative decision-making.
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http://dx.doi.org/10.3389/fonc.2021.592927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276053PMC
June 2021

Ultrasonic desulfurization of amphiphilic magnetic-Janus nanosheets in oil-water mixture system.

Ultrason Sonochem 2021 Aug 7;76:105662. Epub 2021 Jul 7.

Liaoning Shihua University, Fushun 113001, China.

FeO was obtained by reacting FeCl and FeCl with polyethylene glycol, and labeled onto a amphiphilic Janus nanosheet. It was confirmed by infrared spectroscopy, SEM, AFM and EDS that the FeO nanoparticles changed from hydrophilic to amphiphilic. The oxidative desulfurization performance of amphiphilic iron oxide was studied. Results showed that the Janus nanosheets labeled with FeO could significantly improve the removal rate of thiophene sulfide in simulated oil synergistically with ultrasonic waves, and the desulfurization rate could reach 100%. Further, the effect of ultrasound on the sensing ability of the oil-water interface was studied and the ultrasonic attenuation coefficient was calculated. In addition to the desulfurization mechanism of FeO, it was found that although the ultrasonic attenuation coefficient of the amphiphilic nanosheets was high, the number of hydroxyl radicals determined the desulfurization efficiency. The amphiphilic Fe ions were more favorable for the formation of hydroxyl radicals than the single hydrophilic ones.
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http://dx.doi.org/10.1016/j.ultsonch.2021.105662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281658PMC
August 2021

sp. nov. and sp. nov., two new members of the family .

Int J Syst Evol Microbiol 2021 Jul;71(7)

Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, PR China.

Four obligatory anaerobic, Gram-stain-positive, non-motile and rod-shaped organisms (HF-1365, HF-1362, HF-1101 and HF-4214) were isolated from faecal samples of healthy Chinese subjects. Results of 16S rRNA gene sequence analyses showed that these isolates belong to the genera (strains HF-1365 and HF-1362) and (strains HF-1101 and HF-4214), closest to (both 98.6 %) and (98.0 and 97.8 %), respectively. The whole genome sequences of strains HF-1365 and HF-1101 were 2.3 and 4.2 Mb in size with 61.7 and 66.2 mol% DNA G+C content, respectively. The average nucleotide identity and digital DNA-DNA hybridization values indicated that strains HF-1365 and HF-1101 represent novel species in the genera and . Major fatty acid constituents (>10 %) of strains HF-1365 and HF-1362 were C (24.7 and 23.9 %), C (21.9 and 20.6 %) and summed feature 1 (Ciso H/C 3OH; 12.8 and 10.8 %); those of strains HF-1101 and HF-4214 were C 9c (32.4 and 33.1 %) and C (13.9 and 14.0 %). Strain HF-1365 had phospholipid, glycolipid, lipid and phosphoglycolipid without any known quinones, while strain HF-1101 had diphosphatidylglycerol as the major polar lipid and MK-7 (80.7 %) as the predominant quinone. On the basis of their phylogenetic and phenotypic characteristics, strains HF-1365 and HF-1101 represent two distinct species, respectively, in the genera and , for which the names sp. nov. (type strain HF-1365=CGMCC 1.17435=GDMCC 1.1705=JCM 33601) and sp. nov. (type strain HF-1101=CGMCC 1.17436=GDMCC 1.1668=JCM 33773) are proposed.
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http://dx.doi.org/10.1099/ijsem.0.004840DOI Listing
July 2021

Supramolecular Anchoring Strategy for Facile Production of Ruthenium Nanoparticles Embedded in N-Doped Mesoporous Carbon Nanospheres for Efficient Hydrogen Generation.

ACS Appl Mater Interfaces 2021 Jul 12;13(28):32997-33005. Epub 2021 Jul 12.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.

Because of the favorable mass transport and increased available active sites, the rational design and preparation of porous carbon structures are essential but still challenging. Herein, a novel and facile supramolecular anchoring strategy was developed to achieve the embedding of ruthenium (Ru) nanoparticles in N-doped mesoporous carbon nanospheres through pyrolyzing the precursor formed by coordination assembly between metal ions and zinc gluconate (G(Zn)). Featuring rich hydroxyl groups, the G(Zn) can effectively chelate Ru via metal-oxygen bonds to form 3D supramolecular nanospheres, and meanwhile, mesopores in carbon nanospheres were expanded after subsequent pyrolysis thanks to the volatilization of zincic species at high temperature. As a demonstration, the best-performing catalyst displayed extraordinary activity for the hydrogen evolution reaction (HER) with a small overpotential of 43 mV versus reversible hydrogen electrode (vs RHE) at 10 mA/cm and a Tafel slope of 39 mV/dec, which was superior to that of commercial Pt/C in alkaline medium. Theoretical calculations revealed that the catalytic activity was significantly promoted by the strong electronic coupling between Ru nanoparticles and N-doped porous carbon, which increased the electron transfer capability and facilitated the adsorption and dissociation of HO to realize an efficient HER.
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http://dx.doi.org/10.1021/acsami.1c07435DOI Listing
July 2021

Comprehensive Analysis of the Transcriptome-Wide m6A Methylome in Pterygium by MeRIP Sequencing.

Front Cell Dev Biol 2021 25;9:670528. Epub 2021 Jun 25.

Department of Ophthalmology, Yangpu Hospital, Tongji University School of Medicine, Shanghai, China.

Aim: Pterygium is a common ocular surface disease, which is affected by a variety of factors. Invasion of the cornea can cause severe vision loss. N6-methyladenosine (m6A) is a common post-transcriptional modification of eukaryotic mRNA, which can regulate mRNA splicing, stability, nuclear transport, and translation. To our best knowledge, there is no current research on the mechanism of m6A in pterygium.

Methods: We obtained 24 pterygium tissues and 24 conjunctival tissues from each of 24 pterygium patients recruited from Shanghai Yangpu Hospital, and the level of m6A modification was detected using an m6A RNA Methylation Quantification Kit. Expression and location of , a key m6A methyltransferase, were identified by immunostaining. Then we used m6A-modified RNA immunoprecipitation sequencing (MeRIP-seq), RNA sequencing (RNA-seq), and bioinformatics analyses to compare the differential expression of m6A methylation in pterygium and normal conjunctival tissue.

Results: We identified 2,949 dysregulated m6A peaks in pterygium tissue, of which 2,145 were significantly upregulated and 804 were significantly downregulated. The altered m6A peak of genes were found to play a key role in the Hippo signaling pathway and endocytosis. Joint analyses of MeRIP-seq and RNA-seq data identified 72 hypermethylated m6A peaks and 15 hypomethylated m6A peaks in mRNA. After analyzing the differentially methylated m6A peaks and synchronously differentially expressed genes, we searched the Gene Expression Omnibus database and identified five genes related to the development of pterygium (, , , , and ).

Conclusion: Our research shows that m6A modification plays an important role in the development of pterygium and can be used as a potential new target for the treatment of pterygium in the future.
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http://dx.doi.org/10.3389/fcell.2021.670528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267473PMC
June 2021

Superb Microvascular Imaging Technology Can Improve the Diagnostic Efficiency of the BI-RADS System.

Front Oncol 2021 24;11:634752. Epub 2021 Jun 24.

Department of Medical Ultrasound, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

Background: To explore whether superb microvascular imaging (SMI)SMI can improve the diagnostic efficiency by evaluating the vascular index (VI) and vascular architecture (VA) in breast lesions.

Methods: This is a retrospective study of data collected prospectively for research use. Taking 225 consecutive cases of breast lesions from November 2016 to December 2017 as a training set, the VI values and VA types of benign and malignant lesions were calculated based on the pathological results. Taking 238 consecutive cases of breast lesions from January 2018 to October 2018 as the verification set, the diagnostic sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV) were calculated to compare the diagnostic efficacy.

Results: The training set included 225 breast lesions and the validation set 238 breast lesions. The VI value in the malignant group (10.3 ± 8.0) was significantly higher than that in the benign group (4.3 ± 5.0)(P<0.001). A VI value of 4.05 was used as the diagnostic threshold for differentiating benign from malignant lesions, with a sensitivity of 80.5%, a specificity of 61.9%, an accuracy of 71.1%, a PPV of 62.9%, a NPV of 76.9%, and an area under the curve of 0.758 (0.696-0.819). There was a significant difference in the types of benign and malignant VA (P < 0.001), and the PPV of the root hair-like and crab claw-like VAs were 93.9% and 100.0%, respectively. The diagnostic sensitivity, specificity, accuracy, PPV, NPV and area under the AUC curve were 58.0%, 98.2%, 97.0%, 70.3% and 0.781, respectively (95%CI: 0.719-0.844). SMI combined with conventional ultrasound improved the diagnostic specificity (70.0% 90.0%), accuracy (87.4% 96.6%), and PPV (82.5% 93.2%) without decreasing the diagnostic sensitivity (99.3%), yielded higher diagnostic performance with the area under the ROC curve was 0.941 (95%CI: 0904-0.979) compared with conventional US alone (P < 0.001).

Conclusion: A VI value 4.05 is a cut-off value with good diagnostic efficacy. The residual root-like and crab claw-like VAs are the characteristic VAs of malignant lesions. Conventional ultrasound combined with the VI and VA can improve the diagnostic specificity, accuracy and PPV without reducing the diagnostic sensitivity.
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http://dx.doi.org/10.3389/fonc.2021.634752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263934PMC
June 2021

Cation-Disordered O3-NaNiSbO Cathode for High-Voltage Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2021 Jul 9;13(28):32948-32956. Epub 2021 Jul 9.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

O3-type sodium-layered oxides (such as antimony-based O3 structures) have been suggested as one of the most fascinating cathode materials for sodium-ion batteries (SIBs). Honeycomb-ordered antimony-based O3 structures, however, unsatisfactorily exhibit complex phase transitions and sluggish Na kinetics during cycling. Herein, we prepared a completely cationic-disordered O3-type NaNiSbO compound by composition regulation for SIBs. Surprisingly, the measured redox potentials for typical O3-P3 phase transition are located at 3.4 V. Operando X-ray diffraction confirms a reversible phase transition process from the O3 to P3 structure accompanied with a very small volume change (1.0%) upon sodium extraction and insertion. The low activation barrier energy of 400 meV and the fast Na migration of 10 cm·s are further obtained by first-principles calculations and galvanostatic intermittent titration technique, respectively. As a result, the O3-NaNiSbO cathode displays a reversible capacity of 106 mA h g at 0.1C (12 mA g), smooth charge-discharge curves, and a high average working voltage of 3.5 V during battery cycling. The results highlight the importance of searching for a new O3-type structure with cation-disordering and high working voltage for realizing high energy SIBs.
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http://dx.doi.org/10.1021/acsami.1c06576DOI Listing
July 2021

Smart ion imprinted polymer for selective adsorption of Ru(Ⅲ) and simultaneously waste sample being transformed as a catalyst.

J Hazard Mater 2021 Sep 15;417:126072. Epub 2021 May 15.

State Key Laboratory of Nickel and Cobalt Resources Comprehensive Utilization, Jinchang 737100, Gansu, China.

In this work, a temperature-sensitive block polymer PDEA-b-P(DEA-co-AM) was synthesized and then introduced into the preparation of a smart Ru(Ⅲ) imprinted polymer (Ru-IIP) to selectively adsorption Ru(Ⅲ) first. Then the waste Ru-IIP was converted into a catalyst in-situ for recycle. The structure and morphology of the prepared polymer were characterized by Fourier transform infrared spectrometer, Scanning electron microscope, BET surface area and Thermogravimetric analysis. The adsorption properties of the synthesized smart material were investigated in terms of adsorption pH, adsorption kinetics and adsorption isotherm. Results documented that the optimal adsorption temperature and pH were 35 °C and 1.5 respectively, the maximum adsorption capacity was 0.153 mmol/g, and the adsorption processes of Ru-IIP were more suitable to be expressed by pseudo-first-order kinetic and Langmuir model. The selectivity studied in different binary mixed solutions showed that Ru-IIP has good selectivity, and reusability results showed that Ru-IIP still maintains a good adsorption effect after 8 cycles. In addition, the waste Ru-IIP, a Ru(Ⅲ) remained waste sample was employed as the catalyst for the synthesis of imines, and result showed the mass of adsorbent would reduce after the completion of catalysis, which could not only catalyze the reaction but also reduce pollution.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126072DOI Listing
September 2021

Efficacy of bevacizumab in the treatment of pterygium: An updated meta-analysis of randomized controlled trials.

Int Immunopharmacol 2021 Jul 2;98:107921. Epub 2021 Jul 2.

Department of Ophthalmology, Yangpu hospital, School of Medicine, Tongji University, Shanghai 200092, China. Electronic address:

Recurrence is the most common problem following pterygium surgery. Whether bevacizumab can prevent pterygium recurrence is controversial. To address this point, we carried out a meta-analysis of randomized controlled trials evaluating the efficacy and safety of bevacizumab in the treatment of pterygium. We searched the PubMed, EMBASE, Cochrane Library, Web of Science, Chinese Biomedical Literature, China National Knowledge Infrastructure, and Wan fang databases up to September 20, 2020 for relevant articles. We used the Cochrane assessment tool to evaluate the methodologic quality of the included studies, and calculated the relative risk (RR) and 95% confidence interval (CI) of the reported recurrence and complication rates. A total of 17 studies including 1124 patients with 1144 eyes were included in the meta-analysis. The combined results showed that bevacizumab significantly reduced the recurrence rate of pterygium after surgery (RR = 0.652, 95% CI: 0.504-0.845, Z = 3.24, P = 0.001) and was not significantly associated with the occurrence of postoperative complications compared to control treatments (RR = 0.832, 95% CI: 0.604-1.145, Z = 1.13, P = 0.259). A subgroup analysis showed that the rate of pterygium recurrence was significantly lower with bevacizumab than in the control group at a dose of 2.5 mg (RR = 0.47, 95% CI: 0.24-0.91) administered by subconjunctival injection (RR = 0.54, 95% CI: 0.39-0.75) after a follow-up time of ≤ 6 months (RR = 0.63, 95% CI: 0.45-0.88). Thus, bevacizumab can reduce the risk of pterygium recurrence after surgery, and does not differ from placebo or other drug treatments in terms of the risk of complications.
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http://dx.doi.org/10.1016/j.intimp.2021.107921DOI Listing
July 2021

Metabolomics and Network Pharmacology-Based Investigation into the Mechanisms Underlying the Therapeutic Effect of a New Chinese Traditional Medicine (Cui Nai Ling) on Bromocriptine-Induced Hypogalactia.

Evid Based Complement Alternat Med 2021 16;2021:8857449. Epub 2021 Jun 16.

Experimental Teaching Center, College of Chinese Materia Medica and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong 030619, China.

As a traditional veterinary medicine to promote lactation, Cui Nai Ling (CNL) can not only increase milk supply and promote health but also improve the overall physiological function and immunity of the animals. In order to further improve CNL's effect on lactation, we have previously made a new CNL (NCNL) by adding Tetrapanacis Medulla and replacing Vaccariae Semen with fried Vaccariae Semen in CNL. We have demonstrated that the lactation-promoting effect of NCNL is better than that of CNL. However, the underlying mechanisms by which NCNL promotes lactation are unclear. In this study, we performed metabolomics, network pharmacology, and pharmacodynamic studies to explore the underlying mechanisms by which NCNL promotes lactation in rats with bromocriptine-induced hypogalactia. The results showed that NCNL significantly improved the loss of appetite in female adult rats and the weight loss of pups caused by the disorder of lactation. Biochemical analysis showed that NCNL could regulate the levels of PRL, T4, E2, Ca, UREA, GLU, ALT, AST, TCHO, and TG in serum. The pathological results showed that NCNL could promote lactation and increase the mammary gland index by improving breast acinar tissue morphology in rats with hypogalactia. Network pharmacology studies showed that NCNL promotes lactation through P13K-Akt, insulin resistance, and prolactin signaling pathways, among which the most frequently affected pathway was the P13K-Akt signaling pathway. Metabolomics studies showed that NCNL can significantly upregulate phenylalanine, tyrosine, and tryptophan biosynthesis and tyrosine metabolism pathways and downregulate cysteine and methionine metabolism pathways. NCNL can significantly increase the serum prolactin concentration, improve the glucose and lipid metabolism disorders, and regulate PI3K-Akt, insulin resistance, and prolactin pathways to affect the amino acids' metabolism in the mammary gland and ultimately exert its therapeutic effect on bromocriptine-induced postpartum hypogalactia. These findings revealed the effect and application value of NCNL on animals with postpartum hypogalactia.
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http://dx.doi.org/10.1155/2021/8857449DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221871PMC
June 2021

Intergenerational Emotional Cohesion and Psychological Well-being of Older Adults in Rural China: A Moderated Mediation Model of Loneliness and Friendship Ties.

J Gerontol B Psychol Sci Soc Sci 2021 Jul 2. Epub 2021 Jul 2.

Syracuse University.

Objectives: Although researchers have linked intergenerational emotional cohesion (IEC) to psychological well-being (PWB) among older adults, the mechanisms and conditions under which IEC is related to PWB-particularly in rural areas-are less well understood. This study analyzed data from rural China to examine whether loneliness mediated the relation between IEC and PWB, and whether friendship ties moderated the strength of the direct and indirect relationships between IEC and PWB.

Methods: Mediation and moderated mediation models were tested using a sample of rural adults age 60 and older (N = 958) from the Longitudinal Study of Older Adults in Anhui Province, China. Measures included IEC, friendship ties, loneliness, and two PWB indicators-depressive symptoms and life satisfaction.

Results: The results revealed that IEC was negatively related to loneliness, which in turn was associated with depressive symptoms and life satisfaction. Further, this indirect pathway linking IEC and depressive symptoms (but not life satisfaction) was positively conditioned on the size of friendship ties.

Discussion: This study advances our understanding of the mechanism through which IEC influences PWB in older adults. Alleviating loneliness could help boost PWB. Other implications for practice and future research are discussed.
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http://dx.doi.org/10.1093/geronb/gbab122DOI Listing
July 2021

TPD7 inhibits the non-small cell lung cancer HCC827 cell growth by regulating EGFR signalling pathway.

J Chemother 2021 Jul 1:1-7. Epub 2021 Jul 1.

Department of Oncology, Xi'an International Medical Center Hospital, Xi'an, Shaanxi, China.

Non-small cell lung cancer (NSCLC) accounts for 85% of lung cancer cases, and is characterized by more insensitivity to chemotherapy and poor prognosis. Epidermal growth factor receptor (EGFR) has been confirmed as a tumorigenic driving factor of NSCLC. Taspine has been proved effective in the inhibition of malignant tumours. Here, we found TPD7, a novel taspine derivative, exerted most inhibitory effect on EGFR-dependent HCC827 cells and investigated the underling mechanism. In addition, TPD7 could block cell cycle at G0/G1 phase of HCC827 cells by regulating the expression of cyclin D1 and cyclin E. Furthermore, TPD7 induced HCC827 cell apoptosis by regulating the expression of BCL-2 family proteins. Further study revealed that TPD7 could down-regulate the phosphorylation of EGFR and downstream members. TPD7 might present a potential EGFR inhibitor in the treatment of NSCLC.
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http://dx.doi.org/10.1080/1120009X.2021.1945790DOI Listing
July 2021

Monitoring Coronavirus Disease 2019: A Review of Available Diagnostic Tools.

Front Public Health 2021 7;9:672215. Epub 2021 Jun 7.

Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Coronavirus disease 2019 (COVID-19) pneumonia is caused by the virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has rapidly become a global public health concern. As the new type of , SARS-CoV-2 can spread across species and between populations and has a greater risk of transmission than other coronaviruses. To control the spread of SARS-CoV-2, it is vital to have a rapid and effective means of diagnosing asymptomatic SARS-CoV-2-positive individuals and patients with COVID-19, an early isolation protocol for infected individuals, and effective treatments for patients with COVID-19 pneumonia. In this review, we will summarize the novel diagnostic tools that are currently available for coronavirus, including imaging examinations and laboratory medicine by next-generation sequencing (NGS), real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) analysis, immunoassay for COVID-19, cytokine and T cell immunoassays, biochemistry and microbiology laboratory parameters in the blood of the patients with COVID-19, and a field-effect transistor-based biosensor of COVID-19. Specifically, we will discuss the effective detection rate and assay time for the rRT-PCR analysis of SARS-CoV-2 and the sensitivity and specificity of different antibody detection methods, such as colloidal gold and ELISA using specimen sources obtained from the respiratory tract, peripheral serum or plasma, and other bodily fluids. Such diagnostics will help scientists and clinicians develop appropriate strategies to combat COVID-19.
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http://dx.doi.org/10.3389/fpubh.2021.672215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215441PMC
June 2021

Assessment of Exenatide loaded Biotinylated Trimethylated Chitosan/HP-55 Nanoparticles.

Curr Drug Deliv 2021 Jun 13. Epub 2021 Jun 13.

Department of Pharmacy, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, 365 Renmin East Road, Jinhua321000, China.

Background: Exenatide (EXE) is an anti-hyperglycemic agent approved for treating type 2 diabetes by the Food and Drug Administration (FDA). However, twice-daily injection of exenatide inconveniences most patients.

Objective: In this study, biotinylated trimethylated chitosan (Bio-TMC) based nanoparticles were proposed to promote oral absorption of exenatide. Realizing the oral administration of exenatide is very important to alleviate patient suffering and improve patient compliance.

Methods: Bio-TMC was synthesized, and the chemical structure was characterized by Fourier transform infrared (FT-IR) spectroscopy and 1H NMR spectroscopy. Nanoparticles were prepared through polyelectrolyte interaction in the presence of sodium tripolyphosphate (TPP) and Hydroxypropyl methylcellulose phthalate (HP-55). The formulations were physically and chemically characterized. In vitro release was investigated in different pH media. In vivo antidiabetic activities of biotin modified and non-biotin modified chitosan were evaluated in db/db mice.

Results: EXE-loaded Bio-TMC/HP-55 nanoparticles were spherical in shape with a mean diameter of 156.2 nm and zeta potential of +11.3 mV. The drug loading efficiency and loading contents were 52.38% and 2.08%, respectively. In vitro release revealed that EXE-loaded Bio-TMC/HP-55 nanoparticles were released faster in pH 1.2 than pH 6.8 (63.71% vs. 50.12%), indicating that nanoparticles had enteric characteristics. Antidiabetic activity study revealed that after oral administration to diabetic mice, the relative pharmacological bioavailability (FPharm%) of the biotin modified nanoparticles was found to be 1.27-fold higher compared with the unmodified ones and the hypoglycemic effect was also better.

Conclusion: Bio-TMC/HP-55 nanoparticles are feasible as oral drug carriers of exenatide and have the potential to be extended to other drugs that are not readily oral, such as monoclonal antibodies, vaccines, genes, etc., thus, this would be beneficial for pharmaceutical industries. Further research will focus on the biodistribution of Bio-TMC/HP-55 nanoparticles after oral administration.
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http://dx.doi.org/10.2174/1567201818666210614100603DOI Listing
June 2021

Exploring the Pathogenesis of Psoriasis Complicated With Atherosclerosis Microarray Data Analysis.

Front Immunol 2021 27;12:667690. Epub 2021 May 27.

Department of Dermatology, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Background: Although more and more evidence has supported psoriasis is prone to atherosclerosis, the common mechanism of its occurrence is still not fully elucidated. The purpose of this study is to further explore the molecular mechanism of the occurrence of this complication.

Methods: The gene expression profiles of psoriasis (GSE30999) and atherosclerosis (GSE28829) were downloaded from the Gene Expression Omnibus (GEO) database. After identifying the common differentially expressed genes (DEGs) of psoriasis and atherosclerosis, three kinds of analyses were performed, namely functional annotation, protein-protein interaction (PPI) network and module construction, and hub gene identification and co-expression analysis.

Results: A total of 94 common DEGs (24 downregulated genes and 70 upregulated genes) was selected for subsequent analyses. Functional analysis emphasizes the important role of chemokines and cytokines in these two diseases. In addition, lipopolysaccharide-mediated signaling pathway is closely related to both. Finally, 16 important hub genes were identified using cytoHubba, including LYN, CSF2RB, IL1RN, RAC2, CCL5, IRF8, C1QB, MMP9, PLEK, PTPRC, FYB, BCL2A1, LCP2, CD53, NCF2 and TLR2.

Conclusions: Our study reveals the common pathogenesis of psoriasis and atherosclerosis. These common pathways and hub genes may provide new ideas for further mechanism research.
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http://dx.doi.org/10.3389/fimmu.2021.667690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190392PMC
May 2021

Reshaping the active pocket of promiscuous lactonases for degrading bulky organophosphate flame retardants.

Chem Commun (Camb) 2021 Jul;57(53):6475-6478

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.

Engineering of a promiscuous lactonase via semi-rational evolution gave a 1007-fold improvement in its catalytic activity in the degradation of triphenyl phosphate (TPHP). TPHP is a typical bulky organophosphate flame retardant (OPFR) and is widely used in industry. To the best of our knowledge, this is the first artificial enzyme capable of degrading OPFRs.
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http://dx.doi.org/10.1039/d1cc02657gDOI Listing
July 2021

Short sleep duration increases the risk of hyperuricemia among Chinese adults: findings from the China Health and Nutrition Survey.

Sleep Med 2021 May 21;84:40-45. Epub 2021 May 21.

Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China; Shanghai Medical Center of Kidney Disease, Shanghai, 200032, China; Kidney and Dialysis Institute of Shanghai, Shanghai, 200032, China; Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, 200032, China. Electronic address:

Study Objectives: Hyperuricemia is a growing public health problem with its increasing prevalence. Few studies have investigated the association between sleep duration and hyperuricemia. The objective of this study is to explore whether short sleep duration is an independent risk factor of hyperuricemia in Chinese adults.

Methods: The data we analyzed was extracted from the 2009 wave of the China Health and Nutrition Survey. The population we analyzed included 8289 participants aged 18 years or older with sleep of 5-10 h per 24 h. We categorized the population into three groups by sleep duration: 5-6 h (short sleeper),7-8 h (regular sleeper), and 9-10 h (long sleeper). Hyperuricemia was defined as serum uric acid ≥7 mg/dL in men and ≥6 mg/dL in women.

Results: Among the three groups, 9.8% were short sleepers, 68.4% were regular sleepers and 21.8% were long sleepers. The prevalences of hyperuricemia were 19.5%,15.2% and 15.5% respectively. The risks of hyperuricemia in regular and long sleep groups were lower than short sleep group, and the association remained after adjusting for indexes including age, gender, chronic kidney disease, hypertension, diabetes mellitus, high low-density lipoprotein cholesterol (LDL-c), and obesity. In subgroup analysis, we found the association was still observed in participants without hypertension, diabetes mellitus or obesity.

Conclusions: Our findings suggest that short sleep duration is associated with higher risk of hyperuricemia independently of cardiometabolic risk factors, especially in individuals without traditional hyperuricemia risk factors.
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http://dx.doi.org/10.1016/j.sleep.2021.05.014DOI Listing
May 2021

3D Yolk-Shell Structured Si/void/rGO Free-Standing Electrode for Lithium-Ion Battery.

Materials (Basel) 2021 May 26;14(11). Epub 2021 May 26.

State Key Laboratory of Material-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, China.

In this study, we have successfully prepared a free-standing Si/void/rGO yolk-shell structured electrode via the electrostatic self-assembly using protonated chitosan. When graphene oxide (GO) is dispersed in water, its carboxyl and hydroxyl groups on the surface are ionized, resulting in the high electronegativity of GO. Meanwhile, chitosan monomer contains -NH and -OH groups, forming highly electropositive protonated chitosan in acidic medium. During the electrostatic interaction between GO and chitosan, which results in a rapid coagulation phenomenon, Si/SiO nanoparticles dispersed in GO can be uniformly encapsulated between GO sheets. The free-standing Si/void/rGO film can be obtained by freeze-drying, high-pressure compression, thermal reduction and HF etching technology. Our investigation shows that after 200 charge/discharge cycles at the current density of 200 mA·g, the specific discharge capacity of the free-standing electrode remains at 1129.2 mAh·g. When the current density is increased to 4000 mA·g, the electrode still has a specific capacity of 469.2 mAh·g, showing good rate performance. This free-standing electrode with a yolk-shell structure shows potential applications in the field of flexible lithium-ion batteries.
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http://dx.doi.org/10.3390/ma14112836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199465PMC
May 2021

High-Strength GO/PA66 Nanocomposite Fibers via In Situ Precipitation and Polymerization.

Polymers (Basel) 2021 May 22;13(11). Epub 2021 May 22.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, China.

The uniform dispersion of graphene oxide (GO) and strong interfacial bonding are the key factors in achieving the high mechanical strength of GO/polymer composites. It is still challenging to prepare GO/PA66 composites with uniform GO dispersion by the in situ polymerization method. In this paper, we prepare GO/PA66 salt nanocomposite by in situ precipitating PA66 salt with GO in ethanol. The GO/PA66 nanocomposite fibers are then fabricated using the as-prepared GO/PA66 salt by in situ polymerizing and melt spinning. By tuning the GO content, the tensile strength and Young's modulus of the GO/PA66 fibers are increased from 265 ± 18 to 710 ± 14 MPa (containing 0.3 wt% GO) and from 1.1 ± 0.08 to 3.8 ± 0.19 GPa (containing 0.5 wt% GO), respectively. The remarkable improvements are attributed to the uniform dispersion of GO in the GO/PA66 salt nanocomposite via ionic bonding and hydrogen bonding in the in situ precipitation process, and the covalent interfacial bonding between the GO and PA66 during the in situ polymerization process. This work sheds light on the easy fabrication of high-performance PA66-based nanocomposites.
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http://dx.doi.org/10.3390/polym13111688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196895PMC
May 2021

Alterations of Golgi Structural Proteins and Glycosylation Defects in Cancer.

Authors:
Xiaoyan Zhang

Front Cell Dev Biol 2021 12;9:665289. Epub 2021 May 12.

College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, China.

As the central hub in the secretory and endocytic pathways, the Golgi apparatus continually receives the flow of cargos and serves as a major processing station in the cell. Due to its dynamic nature, a sophisticated and constantly remodeling mechanism needs to be set up to maintain the Golgi architecture and function in the non-stop trafficking of proteins and lipids. Abundant evidence has been accumulated that a well-organized Golgi structure is required for its proper functions, especially protein glycosylation. Remarkably, altered glycosylation has been a hallmark of most cancer cells. To understand the causes of Golgi defects in cancer, efforts have been made to characterize Golgi structural proteins under physiological and pathological conditions. This review summarizes the current knowledge of crucial Golgi structural proteins and their connections with tumor progression. We foresee that understanding the Golgi structural and functional defects may help solve the puzzle of whether glycosylation defect is a cause or effect of oncogenesis.
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http://dx.doi.org/10.3389/fcell.2021.665289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149618PMC
May 2021

Screening for mutants with altered Ca signal response using aequorin-based Ca reporter system.

STAR Protoc 2021 Jun 23;2(2):100558. Epub 2021 May 23.

Shanghai Center for Plant Stress Biology, and CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

Environmental stimuli evoke transient increases of the cytosolic Ca level. To identify upstream components of Ca signaling, we have optimized two forward genetic screening systems based on Ca reporter aequorin. AEQ and AEQ plants were used for generating ethyl methanesulfonate (EMS)-mutagenized libraries. The AEQ EMS-mutagenized library was preferably used to screen the mutants with reduced Ca signal response due to its high effectiveness, while the AEQ EMS-mutagenized library was used for screening of the mutants with altered Ca signal response. For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) and Zhu et al. (2013).
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http://dx.doi.org/10.1016/j.xpro.2021.100558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144734PMC
June 2021

CARD11 is a novel target of miR-181b that is upregulated in chronic lymphocytic leukemia.

Biomark Med 2021 Jun 27;15(9):623-635. Epub 2021 May 27.

Department of Hematology, The People's Hospital of Xin jiang Uygur Autonomous Region, Urumqi, China.

To investigate the targets of miR-181b in patients with chronic lymphocytic leukemia (CLL). The bioinformatic softwares were used to indicate the key target genes associated with miR-181b, and the results were verified in CLL patient samples and 293T cells.  is a potential target gene of miR-181b, an inverse relationship was revealed between the expression of CARD11 and miR-181b in 104 CLL patients, and it was confirmed with luciferase assays and western blotting. Kaplan-Meier analysis showed that CLL patients with high CARD11 expression demonstrated poor survival. is a novel target of miR-181b that is upregulated, which could be a poor prognostic indicator for CLL patients.
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http://dx.doi.org/10.2217/bmm-2020-0601DOI Listing
June 2021

Preserved skeletal muscle oxidative capacity in older adults despite decreased cardiorespiratory fitness with ageing.

J Physiol 2021 Jul 11;599(14):3581-3592. Epub 2021 Jun 11.

Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, MN, USA.

Key Points: Healthy older adults exhibit lower cardiorespiratory fitness ( ) than young in the absence of any age-related difference in skeletal muscle mitochondrial capacity, suggesting central haemodynamics plays a larger role in age-related declines in . Total physical activity did not differ by age, but moderate-to-vigorous physical activity was lower in older compared to young adults. Moderate-to-vigorous physical activity is associated with and muscle oxidative capacity, but physical inactivity cannot entirely explain the age-related reduction in .

Abstract: Declining fitness ( ) is a hallmark of ageing and believed to arise from decreased oxygen delivery and reduced muscle oxidative capacity. Physical activity is a modifiable lifestyle factor that is critical when evaluating the effects of age on parameters of fitness and energy metabolism. The objective was to evaluate the effects of age and sex on , muscle mitochondrial physiology, and physical activity in young and older adults. An additional objective was to assess the contribution of skeletal muscle oxidative capacity to age-related reductions in and determine if age-related variation in and muscle oxidative capacity could be explained on the basis of physical activity levels. In 23 young and 52 older men and women measurements were made of , mitochondrial physiology in permeabilized muscle fibres, and free-living physical activity by accelerometry. Regression analyses were used to evaluate associations between age and , mitochondrial function, and physical activity. Significant age-related reductions were observed for (P < 0.001), but not muscle mitochondrial capacity. Total daily step counts did not decrease with age, but older adults showed lower moderate-to-vigorous physical activity, which was associated with (R  = 0.323, P < 0.001) and muscle oxidative capacity (R  = 0.086, P = 0.011). After adjusting for sex and physical activity, age was negatively associated with but not muscle oxidative capacity. Healthy older adults exhibit lower but preserved mitochondrial capacity compared to young. Physical activity, particularly moderate-to-vigorous, is a key factor in observed age-related changes in fitness and muscle oxidative capacity, but cannot entirely explain the age-related reduction in .
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http://dx.doi.org/10.1113/JP281691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284426PMC
July 2021

Secondary hemicrania continua-tic syndrome associated with fungal sphenoiditis: A case report.

Clin Case Rep 2021 May 19;9(5):e04297. Epub 2021 May 19.

Department of Neurology The 940th Hospital of Chinese PLA Lanzhou China.

The coexistence of hemicrania-continua and trigeminal neuralgia is called HC-tic syndrome. We describe a case of an elderly man who suffered both types of headache related to fungal sphenoiditis. This is the first case to be reported, to the best of our knowledge.
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http://dx.doi.org/10.1002/ccr3.4297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133077PMC
May 2021

An active marine halophenol derivative attenuates lipopolysaccharide-induced acute liver injury in mice by improving M2 macrophage-mediated therapy.

Int Immunopharmacol 2021 Jul 20;96:107676. Epub 2021 May 20.

School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China; Shanxi Key Laboratory of Innovative Drug for the Treatment of Serious Diseases Basing on the Chronic Inflammation,Shanxi University of Chinese Medicine, Taiyuan 030619, PR China. Electronic address:

2,4',5'-Trihydroxyl-5,2'-dibromo diphenylmethanone (LM49), an active halophenol derivative synthesized by our group, which exhibits a broad spectrum of therapeutic properties, such as antioxidant and anti-inflammatory activities. In this study, we found LM49 could obviously attenuate acute liver injury induced by lipopolysaccharide (LPS) in mice by polarizing macrophages. The protective effect was described by reducing the hepatic inflammation and improving hepatic function using aspartate transaminase (AST) and alanine transaminase (ALT) assay. Further study revealed that LM49 pretreatment induced the Kupffer cells (KCs) to M2 polarization and decreased the production of inflammatory cytokines. The action mechanism in RAW 264.7 macrophages showed that LM49 could induce the activation of JAK1/STAT6 signaling pathway and the inhibition of TLR-4/NF-kB axis. Morever, LM49 also upregulated the expression of SOCS1 and FLK-4, which can promote M2 polarization by cooperating with STAT6 and inhibit M1 formation by reducing JAK1/STAT1. Our results suggested that LM49 could protect against LPS-induced acute liver injury in mice via anti-inflammatory signaling pathways and subsequent induction of M2 Kupffer cells. The results provided the first experimental evidence of active halophenols for the anti-inflammatory therapy by targeting M2 macrophages.
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http://dx.doi.org/10.1016/j.intimp.2021.107676DOI Listing
July 2021

Three-Dimensional Reticulated, Spongelike, Resilient Aerogels Assembled by SiC/SiN Nanowires.

Nano Lett 2021 May 17;21(10):4167-4175. Epub 2021 May 17.

State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

For nanofibrous aerogels, a three-dimensional porous structure with interwoven nanofibers as a pore wall has become an urgent demand, and it remains to be a challenge to ensure the mechanical stability and thermal insulation. Other than the reported nanofiber as raw materials to generate three-dimensional cellular nanofibrous aerogels, an alternative low-cost and facile procedure has been proposed here via tactfully utilizing polymer sponge as a template attached with reactive particles, followed by a carbothermal reduction process to realize nanowire growth and their replacement of the original framework. The resulting spongy aerogels with numerous interlaced SiC/SiN nanowires as a skeleton exhibit an ultrahigh porosity of 99.79%. Meanwhile, compressive elasticity after a compression at strain of 35% for 400 cycles, a low thermal conductivity of 23.19 mW/(m K), an excellent absorption capacity of 33.9-95.3 times for varied organic solvents removal, along with flexibility in shape design favored by the initial organic sponge make this nanofibrous aerogel an ideal material for heat shielding, absorption, or catalyst support.
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http://dx.doi.org/10.1021/acs.nanolett.0c04917DOI Listing
May 2021

Adipose tissue macrophage populations and inflammation are associated with systemic inflammation and insulin resistance in obesity.

Am J Physiol Endocrinol Metab 2021 Jul 17;321(1):E105-E121. Epub 2021 May 17.

Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, grid.66875.3aMayo Clinic, Rochester, Minnesota.

Obesity is accompanied by numerous systemic and tissue-specific derangements, including systemic inflammation, insulin resistance, and mitochondrial abnormalities in skeletal muscle. Despite growing recognition that adipose tissue dysfunction plays a role in obesity-related disorders, the relationship between adipose tissue inflammation and other pathological features of obesity is not well-understood. We assessed macrophage populations and measured the expression of inflammatory cytokines in abdominal adipose tissue biopsies in 39 nondiabetic adults across a range of body mass indexes (BMI 20.5-45.8 kg/m). Skeletal muscle biopsies were used to evaluate mitochondrial respiratory capacity, ATP production capacity, coupling, and reactive oxygen species production. Insulin sensitivity (S) and β cell responsivity were determined from test meal postprandial glucose, insulin, c-peptide, and triglyceride kinetics. We examined the relationships between adipose tissue inflammatory markers, systemic inflammatory markers, S, and skeletal muscle mitochondrial physiology. BMI was associated with increased adipose tissue and systemic inflammation, reduced S, and reduced skeletal muscle mitochondrial oxidative capacity. Adipose-resident macrophage numbers were positively associated with circulating inflammatory markers, including tumor necrosis factor-α (TNFα) and C-reactive protein (CRP). Local adipose tissue inflammation and circulating concentrations of TNFα and CRP were negatively associated with S, and circulating concentrations of TNFα and CRP were also negatively associated with skeletal muscle oxidative capacity. These results demonstrate that obese humans exhibit increased adipose tissue inflammation concurrently with increased systemic inflammation, reduced insulin sensitivity, and reduced muscle oxidative capacity and suggest that adipose tissue and systemic inflammation may drive obesity-associated metabolic derangements. Adipose inflammation is proposed to be at the nexus of the systemic inflammation and metabolic derangements associated with obesity. The present study provides evidence to support adipose inflammation as a central feature of the pathophysiology of obesity. Adipose inflammation is associated with systemic and peripheral metabolic derangements, including increased systemic inflammation, reduced insulin sensitivity, and reduced skeletal muscle mitochondrial respiration.
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http://dx.doi.org/10.1152/ajpendo.00070.2021DOI Listing
July 2021
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