Publications by authors named "Xiaoyan Zhang"

1,264 Publications

  • Page 1 of 1

3D printed scaffold for repairing bone defects in apical periodontitis.

BMC Oral Health 2022 Aug 8;22(1):327. Epub 2022 Aug 8.

Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin Stomatological Hospital, The Affiliated Stomatological Hospital of Nankai University, No.75, Dagu Road, Heping District, Tianjin, 300041, China.

Objectives: To investigate the feasibility of the 3D printed scaffold for periapical bone defects.

Methods: In this study, antimicrobial peptide KSL-W-loaded PLGA sustainable-release microspheres ([email protected]) were firstly prepared followed by assessing the drug release behavior and bacteriostatic ability against Enterococcus faecalis and Porphyromonas gingivalis. After that, we demonstrated that [email protected]/collagen (COL)/silk fibroin (SF)/nano-hydroxyapatite (nHA) (COL/SF/nHA) scaffold via 3D-printing technique exhibited significantly good biocompatibility and osteoconductive property. The scaffold was characterized as to pore size, porosity, water absorption expansion rate and mechanical properties. Moreover, MC3T3-E1 cells were seeded into sterile scaffold materials and investigated by CCK-8, SEM and HE staining. In the animal experiment section, we constructed bone defect models of the mandible and evaluated its effect on bone formation. The Japanese white rabbits were killed at 1 and 2 months after surgery, the cone beam computerized tomography (CBCT) and micro-CT scanning, as well as HE and Masson staining analysis were performed on the samples of the operation area, respectively. Data analysis was done using ANOVA and LSD tests. (α = 0.05).

Results: We observed that the [email protected] sustainable-release microspheres prepared in the experiment were uniform in morphology and could gradually release the antimicrobial peptide (KSL-W), which had a long-term antibacterial effect for at least up to 10 days. HE staining and SEM showed that the scaffold had good biocompatibility, which was conducive to the adhesion and proliferation of MC3T3-E1 cells. The porosity and water absorption of the scaffold were (81.96 ± 1.83)% and (458.29 ± 29.79)%, respectively. Histological and radiographic studies showed that the bone healing efficacy of the scaffold was satisfactory.

Conclusions: The [email protected]/COL/SF/nHA scaffold possessed good biocompatibility and bone repairing ability, and had potential applications in repairing infected bone defects. Clinical significance The 3D printed scaffold not only has an antibacterial effect, but can also promote bone tissue formation, which provides an alternative therapy option in apical periodontitis.
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http://dx.doi.org/10.1186/s12903-022-02362-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358902PMC
August 2022

Application of Ultrasound Combined with Magnetic Resonance Imaging in the Diagnosis and Grading of Patients with Prenatal Placenta Accreta.

Scanning 2022 22;2022:1199210. Epub 2022 Jul 22.

Department of Obstetrics and Gynecology Women and Children's Hospital of Chongqing Medical University, China.

In order to study the clinical application value of placenta accreta (PIA) diagnosis and grading, the authors propose a method based on ultrasound combined with magnetic resonance imaging in the diagnosis and grading of prenatal placenta accreta patients. This method is adopted in materials and methods: a retrospective analysis of hospital patients with high suspicion of placenta accreta by clinical or ultrasonography between October 2019 and October 2021, the imaging and clinical data of 312 patients who underwent placental MRI examination. The MRI imaging data of all patients were jointly analyzed, and the main observation indicators are as follows: (1) dark zone in the placenta, (2) disruption of the border of the myometrium, (3) disruption of the myometrium, (4) abnormal blood vessels in the placenta, (5) enlargement of the lower part of the uterus, and (6) local bulge of the bladder/or invasion of the adjacent tissues of the uterus. The results show the following: in MRI combined with ultrasonography ( < 0.05), there was no statistical significance in the specificity and accuracy of MRI combined with ultrasound to diagnose PIA ( > 0.05). The comparison of graded diagnostic accuracy showed that in ultrasound alone < MRI alone < MRI combined with ultrasound, the differences were statistically significant ( < 0.05). Ultrasound combined with MRI in the diagnosis of placenta accreta is in good agreement with the clinical and surgical pathological results; MRI examination can be used as an important method for prenatal placenta accreta screening. MRI can classify placenta accreta to some extent.
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http://dx.doi.org/10.1155/2022/1199210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337953PMC
August 2022

Effect Evaluation of Preoperative Psychological Nursing Intervention on Sinusitis Patients Undergoing General Anesthesia by Multiplanner Reformation-Based CT.

Comput Math Methods Med 2022 28;2022:7516339. Epub 2022 Jul 28.

Day Operating Room, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Guangzhou, 510000 Guangdong, China.

The aim of this study was at exploring the clinical effect of CT images based on multiplanner reformation (MPR) combined with a preoperative psychological nursing intervention model in sinusitis patients undergoing general anesthesia. Sixty sinusitis patients who received MPR-based CT examination and general anesthesia were selected as the study subjects and randomly divided into the control group ( = 30) and the experimental group ( = 30). The control group used traditional preoperative education. The experimental group added the psychological nursing intervention based on traditional preoperative education. The blood pressure and heart rate before and after the operation, the self-rating anxiety scale (SAS) score before and after intervention, and satisfaction were comprehensively assessed. The results showed that CT based on MPR could observe the lesions and anatomical structures of the sinus wall and sinus in detail from multiple angles. The blood pressure (systolic blood pressure 135.12 ± 14.89 mmHg, diastolic blood pressure 87.05 ± 11.24 mmHg), heart rate (78.42 ± 12.19 beats/min), SAS score (45.85 ± 4.97 points), and nursing satisfaction (78.9%) of the experimental group were significantly better than those of the control group (145.83 ± 15.62 mmHg, 94.21 ± 10.86 mmHg, 86.44 ± 13.65 beats/min, 56.44 ± 5.12 points, 56.4%), and the differences were statistically significant ( < 0.05). In summary, the preoperative psychological care model has a positive role in reducing the tension and anxiety of patients before general anesthesia surgery and CT based on MPR is important for the clinical diagnosis and treatment of sinusitis. This study provides a theoretical reference for the clinical treatment of patients with sinusitis.
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http://dx.doi.org/10.1155/2022/7516339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352500PMC
August 2022

Association Between p.R229Q and Focal Segmental Glomerular Sclerosis/Steroid-Resistant Nephrotic Syndrome.

Front Med (Lausanne) 2022 22;9:937122. Epub 2022 Jul 22.

Department of Nephrology, Institute of Nephrology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Aim: is the coding gene of podocin. This study aims to investigate the association between p.R229Q (rs61747728), the most frequently reported missense variant of , and focal segmental glomerular sclerosis (FSGS) or steroid-resistant nephrotic syndrome (SRNS) based on typing the variant in a Chinese FSGS/SRNS cohort and conducting a meta-analysis.

Method: We recruited patients with FSGS or SRNS and healthy individuals. To conduct a meta-analysis, all studies on p.R229Q and FSGS/SRNS were searched from public databases.

Results: In total, we enrolled 204 patients with FSGS, 61 patients with SRNS [46 with FSGS, 9 with minimal change disease (MCD), and six patients with IgA nephropathy (IgAN)], and 100 healthy controls. Unexpectedly, p.R229Q was absent in the patients from our cohort. By meta-analysis of 21 studies including 2,489 patients with FSGS/SRNS and 6,004 healthy controls, we confirmed that the A allele of p.R229Q was significantly associated with increased risk of FSGS/SRNS (allelic OR = 1.9, 95% CI = 1.44-2.52, < 0.001). However, the subgroup analysis showed that the association between p.R229Q and FSGS/SRNS was true only in Caucasians (allelic OR = 2.14, 95%CI = 1.54-2.98, < 0.001) and in early-onset patients (allelic OR: 2.13, 95% CI = 1.21-3.76, = 0.009).

Conclusion: p.R229Q may play an important role in enhancing the susceptibility of FSGS/SRNS, especially in ethnicity of Caucasian and age of early-onset patients.
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http://dx.doi.org/10.3389/fmed.2022.937122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354893PMC
July 2022

Recent Advances in TiO2-based Photoanodes for Photoelectrochemical Water Splitting.

Chem Asian J 2022 Aug 4. Epub 2022 Aug 4.

Shanghai Aerospace Hydrogen Energy Technology Co. Ltd, Department of R & D, CHINA.

Photoelectrochemical (PEC) water splitting has attracted a great attention in the past several decades which holds great promise to address global energy and environmental issues by converting solar energy into hydrogen. However, its low solar-to-hydrogen (STH) conversion efficiency remains a bottleneck for practical application. Developing efficient photoelectrocatalysts with high stability and high STH conversion efficiency is one of the key challenges. As a typical n-type semiconductor, titanium dioxide (TiO 2 ) exhibits high PEC water splitting performance, especially high chemical and photo stability. But, TiO 2 has also disadvantages such as wide band gap and fast electron-hole recombination rate, which seriously hinder its PEC performance. This review focuses on recent development in TiO 2 -based photoanodes as well as some key fundamentals. The corresponding mechanisms and key factors for high STH, and controllable synthesis and modification strategies are highlighted in this review. We conclude finally with an outlook providing a critical perspective on future trends on TiO 2 -based photoanodes for PEC water splitting.
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http://dx.doi.org/10.1002/asia.202200668DOI Listing
August 2022

Extracorporeal Membrane Oxygenation Supported Transbronchial Cryobiopsy in the Diagnosis of Severe Organizing Pneumonia: A Case Report.

Front Med (Lausanne) 2022 14;9:955992. Epub 2022 Jul 14.

Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing China.

This case report describes a 58-year-old, never-smoking housewife with chief complaints of progressively worsening cough, dyspnea, and intermittent fever, who was initially misdiagnosed with community-acquired pneumonia (CAP). However, her pulse oximetry oxygen saturation continued to decline, and eventually, she underwent an endotracheal intubation. Fortunately, transbronchial cryobiopsy (TBCB) assisted by extracorporeal membrane oxygenation (ECMO) was performed in the most critical situation, and it revealed an organizing pneumonia (OP) pattern. OP describes a histological pattern of acute or subacute pulmonary damage, which may be idiopathic or associated with a known or unknown underlying disease. A definitive diagnosis of OP usually obtained from pathology, and surgical lung biopsy with large lung tissue is recommended. However, since the surgical lung biopsy was not convenient for this patient after mechanical ventilation, bedside TBCB supported by ECMO was selected. To our knowledge, we are the first to report the pathological diagnosis of ECMO assisted TBCB in acute respiratory failure. When oxygenation cannot be maintained after endotracheal intubation and surgical lung biopsy is not feasible, ECMO-supported TBCB may be a good choice to obtain lung tissue for histopathological diagnosis in patients with acute lung injury of unknown etiology.
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http://dx.doi.org/10.3389/fmed.2022.955992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329585PMC
July 2022

Shows Unique Patterns of Codon Usage Bias in Conventional Versus Unconventional Clade.

Front Cell Infect Microbiol 2022 14;12:941325. Epub 2022 Jul 14.

Clinical Center for BioTherapy & Institutes of Biomedical Sciences, Zhongshan Hospital, Fudan University, Shanghai, China.

() species cause hand, foot and mouth disease (HFMD), threatening the health of young children. Understanding the mutual codon usage pattern of the virus and its host(s) has fundamental and applied values. Here, through examining multiple codon usage parameters, we found that the codon usage bias among strains varies and is clade-specific. EVA76, EVA89, EVA90, EVA91 and EVA92, the unconventional clade of strains, show unique codon usage pattern relative to the two conventional clades, including EVA71, CVA16, CVA6 and CVA10, Analyses of Effective Number of Codon (ENC), Correspondence Analysis (COA) and Parity Rule 2 (PR2), , revealed that the codon usage patterns of strains are shaped by mutation pressure and natural selection. Based on the neutrality analysis, we determined the dominant role of natural selection in the formation of the codon usage bias of . In addition, we have determined the codon usage compatibility of potential hosts for strains using codon adaptation index (CAI), relative codon deoptimization index (RCDI) and similarity index (SiD) analyses, and found that showed host-specific codon adaptation patterns in different clades. Finally, we confirmed that the unique codon usage pattern of the unconventional clade affected protein expression level in human cell lines. In conclusion, we identified novel characteristics of codon usage bias in distinct clades associated with their host range, transmission and pathogenicity.
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http://dx.doi.org/10.3389/fcimb.2022.941325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329520PMC
August 2022

Messaging about descriptive and injunctive norms can promote honesty in young children.

Child Dev 2022 Jul 29. Epub 2022 Jul 29.

Department of Psychology, University of California San Diego, La Jolla, California, USA.

This research examined the effectiveness of using norms to promote honesty. Participants were Han Chinese children (N = 568, 50.4% male, 3.24 to 6.00 years, collected 2020-2022). Relative to children in a control condition, children in Study 1 were more likely to confess to having cheated in a game after being presented with a descriptive norm indicating that confessions are typical, or an injunctive norm indicating that most other children approve of confessing. Study 2 showed that this finding was not due to a methodological artifact, and Study 3 replicated the effect in a context in which the norm information was conveyed by someone other than the experimenter. The findings suggest that messages about social norms can influence children's honesty.
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http://dx.doi.org/10.1111/cdev.13830DOI Listing
July 2022

Design of ROS-Responsive Hyaluronic Acid-Methotrexate Conjugates for Synergistic Chemo-Photothermal Therapy for Cancer.

Mol Pharm 2022 Jul 28. Epub 2022 Jul 28.

Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.

Combining chemotherapy with photothermal therapy (PTT) for cancer treatment could overcome the inherent limitations of both single-modality chemotherapy and PTT. However, the obstacle of accurate drug delivery to tumor sites based on chemo-photothermal remains challenging. This article describes development of a reactive oxygen species (ROS)-responsive hyaluronic acid-based nanoparticle to overcome these drawbacks. Herein, HA-TK-MTX (HTM) was synthesized by a ROS-responsive cleaved thioketal moiety linker (TK) of methotrexate (MTX) and hyaluronic acid (HA). Through hydrophobic interaction and π-π stacking interaction, a photothermal agent IR780 was integrated into the HTM, and the IR780/HTM nanoparticles (IHTM NPs) were obtained. The IHTM NPs show high photostability, excellent photothermal performance, remarkable tumor-targeting ability, and ROS sensibility. Due to the accurate drug delivery ability and superior chemo-photothermal treatment effect of IHTM NPs, the tumor inhibition rate reached 70.95% for 4T1 tumor-bearing mice. This work serves as a precedent for the chemo-photothermal therapy of cancer by rationally designing ROS-responsive nanoparticles.
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http://dx.doi.org/10.1021/acs.molpharmaceut.2c00472DOI Listing
July 2022

The Effect of Implicit Followership Antecedents on the New Generation of Individuals.

Front Psychol 2022 11;13:933770. Epub 2022 Jul 11.

School of Management, Guangdong University of Science and Technology, Dongguan, China.

This study used the Predisposition Proposition Theory of implicit followership to determine the effect of a proactive personality and core self-evaluation on the implicit followership of different schemas. Intertemporal survey data for one month from 452 university graduates were collected to evaluate that core self-evaluation significantly and positively affects positive implicit followership and significantly and negatively affects negative followership. However, the effect of proactive personality on implicit followership is not significant. The results of data analysis support the interpretation of propensity propositions in the study of personality traits. This study also determines the theoretical significance and practical application value.
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http://dx.doi.org/10.3389/fpsyg.2022.933770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310036PMC
July 2022

Germplasm Sources, Genetic Richness, and Population Differentiation of Modern Chinese Soybean Cultivars Based on Pedigree Integrated With Genomic-Marker Analysis.

Front Plant Sci 2022 11;13:945839. Epub 2022 Jul 11.

Soybean Research Institute & MARA National Center for Soybean Improvement & MARA Key Laboratory of Biology and Genetic Improvement of Soybean (General) & State Key Laboratory for Crop Genetics and Germplasm Enhancement & Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, China.

Soybean is a native crop in China for ≈ 5,000 years. The 560 cultivars released in 2006-2015, commercialized with seeds available publicly, were collected (designated modern Chinese soybean cultivars, MCSCs), as a part of 2,371 ones released during ~100 years' breeding history. The MCSCs with their parental pedigrees were gathered, including 279, 155, and 126 cultivars from Northeast and Northwest China (NNC), Huang-Huai-Hai Valleys (HHH), and Southern China (SC), respectively. The MCSCs were tested in the field, genotyped with sequencing, and analyzed for their germplasm sources, genetic richness, and population differentiation based on pedigree integrated with genomic-marker analysis. The main results were as follows: (i) The MCSCs covering 12 of the global 13 MGs (maturity groups) showing different ecoregions with different cropping systems caused their different MG constitutions. (ii) Parental pedigree analysis showed 718 immediate parents and 604 terminal ancestors involved in MCSCs, from which 41 core-terminal ancestors were identified. (iii) NNC was richer in allele number and specific present/deficient alleles, and genetically distant from HHH and SC. (iv) The geographic grouping of MCSCs was partially consistent with marker-based clustering, indicating multiple genetic backgrounds in three eco-subpopulations. (v) Eleven major core-terminal ancestor-derived families were identified, including four derived from ancestors in NNC, four from HHH, and three from SC, containing 463 (82.68%) MCSCs with some cross-distribution among ecoregions. (vi) CGS (coefficient of genetic similarity) calculated from genomic markers showed more precision than COP (coefficient of parentage) using pedigree information in evaluating genetic relationship/differentiation. Overall, through pedigree and genomic-marker analyses, the germplasm constitutions of the three eco-subpopulations were relatively self-sufficient, and germplasm exchange is seriously required for further improvement.
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http://dx.doi.org/10.3389/fpls.2022.945839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309878PMC
July 2022

Implementation strategies to improve evidence-based practice for post-stroke dysphagia identification and management: A before-and-after study.

Int J Nurs Sci 2022 Jul 18;9(3):295-302. Epub 2022 Jun 18.

School of Nursing, Beijing University of Chinese Medicine, Beijing, China.

Objectives: Even though guidelines are available to guide dysphagia identification and management practice, there is still a gap between evidence and practice, which requires improvement. The purpose of this study was to determine the effect of using tailored, multifaceted strategies to improve evidence-based post-stroke dysphagia identification and management practice in a community hospital.

Methods: Guided by the Knowledge to Action framework, the tailored, multifaceted strategies were developed and implemented for 5 months in a community hospital using a before-and-after study design. These strategies consisted of training intervention, policy intervention, and audit and feedback intervention. Nurses' level of knowledge and adherence, were collected in March 2019 and again in January 2020. Patients' quality of life and satisfaction were evaluated during the pre-intervention period (between February 2019 and April 2019) and the post-intervention period (between November 2019 and January 2020).

Results: A total of 55 patients with post-stroke dysphagia (28 in the pre-intervention period and 27 in the post-intervention period) and 17 registered nurses were recruited. Following implementation, there were statistically significant improvements in patients' outcomes (quality of life and satisfaction) and nurses' outcomes (level of knowledge and adherence).

Conclusions: This study assists in closing the research-practice gap by using tailored, multifaceted strategies to increase the use of evidence-based nursing care for dysphagia identification and management practices.
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http://dx.doi.org/10.1016/j.ijnss.2022.06.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305012PMC
July 2022

PPARβ/δ Augments IL-1β-Induced COX-2 Expression and PGE2 Biosynthesis in Human Mesangial Cells via the Activation of SIRT1.

Metabolites 2022 Jun 27;12(7). Epub 2022 Jun 27.

Health Science Center, East China Normal University, Shanghai 200241, China.

Peroxisome proliferator-activated receptor β/δ (PPARβ/δ), a ligand-activated nuclear receptor, regulates lipid and glucose metabolism and inflammation. PPARβ/δ can exert an anti-inflammatory effect by suppressing proinflammatory cytokine production. Cyclooxygenase-2 (COX-2)-triggered inflammation plays a crucial role in the development of many inflammatory diseases, including glomerulonephritis. However, the effect of PPARβ/δ on the expression of COX-2 in the kidney has not been fully elucidated. The present study showed that PPARβ/δ was functionally expressed in human mesangial cells (hMCs), where its expression was increased by interleukin-1β (IL-1β) treatment concomitant with enhanced COX-2 expression and prostaglandin E2 (PGE2) biosynthesis. The treatment of hMCs with GW0742, a selective agonist of PPARβ/δ, or the overexpression of PPARβ/δ via an adenovirus-mediated approach significantly increased COX-2 expression and PGE2 production. PPARβ/δ could further augment the IL-1β-induced COX-2 expression and PGE2 production in hMCs. Moreover, both PPARβ/δ activation and overexpression markedly increased sirtuin 1 (SIRT1) expression. The inhibition or knockdown of SIRT1 significantly attenuated the effects of PPARβ/δ on the IL-1β-induced expression of COX-2 and PGE2 biosynthesis. Taken together, PPARβ/δ could augment the IL-1β-induced COX-2 expression and PGE2 production in hMCs via the SIRT1 pathway. Given the critical role of COX-2 in glomerulonephritis, PPARβ/δ may represent a novel target for the treatment of renal inflammatory diseases.
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http://dx.doi.org/10.3390/metabo12070595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320509PMC
June 2022

The Paradoxical Effects of COVID-19 Event Strength on Employee Turnover Intention.

Int J Environ Res Public Health 2022 Jul 10;19(14). Epub 2022 Jul 10.

School of Business, Qingdao University, Qingdao 266071, China.

As a global pandemic, the novel coronavirus (COVID-19) has brought enormous challenges to employees and organizations. Although numerous existing studies have highlighted that the COVID-19 pandemic is a stressful event and empirically proved its detrimental effect on employee turnover intention, few scholars have noted that this pandemic can deteriorate the external economic and employment environment simultaneously, which may further complicate employees' intentions to leave or stay in the current organization. Drawing on event system theory and social cognitive theory, this study aims to uncover two potential cognitive mechanisms of the complex impact of COVID-19 event strength on employee turnover intention. To examine the proposed model, this study employed a three-wave and time-lagged research design and collected data from a sample of 432 employees of four Chinese companies from different industries. The findings indicated that COVID-19 event strength was negatively related to perceived external employability, and ultimately curbed employee turnover intention. Yet, COVID-19 event strength also negatively predicted perceived organizational growth, thus influencing employees to exhibit intentions to quit. Moreover, organizational identification not only attenuated the positive effect of perceived external employability on turnover intention but also amplified the negative impact of perceived organizational growth on turnover intention. Further, organizational identification moderated the indirect effects of COVID-19 event strength on turnover intention through perceived external employability and perceived organizational growth. This study provided a comprehensive insight into scholars' understanding of the COVID-19 downstream outcomes.
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http://dx.doi.org/10.3390/ijerph19148434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319035PMC
July 2022

Designing waste Bioresource-derived value-added Nanohybrids for efficient photocatalysis water treatment.

Chemosphere 2022 Jul 21:135789. Epub 2022 Jul 21.

The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362400, Fujian Province, PR China.

Although photocatalysis with ultraviolet-visible (UV-vis) light has made considerable advances, it is limited by the low efficiency of UV-vis energy conversion. To overcome this problem, UV-vis light can be replaced with near-infrared (NIR) light. Herein, we coupled eggshell-derived CaCO with a NIR-absorbing CuSe semiconductor and fabricated an insulator-based heterojunction structure. In application case studies of 4-nitrophenol (4-NP) and bacteria, the nanocomposites showed enhanced photocatalysis activity under NIR light induction. A first-principles calculation indicated that photoexcited electrons could transfer from the conduction band of CuSe to the conduction band of CaCO. The main reactive species generated by the photocatalysis were ·CO, and ·OH free radicals. The antibacterial mechanisms of photocatalysis on the cell permeability and DNA layers of the bacterial cells were also revealed. Besides providing novel perspectives and mechanistic understanding of the fabrication of NIR light-driven photocatalysts, this study demonstrates the valorization of eggshell bio-wastes in environmental remediation.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135789DOI Listing
July 2022

Identification of heptapeptides targeting a lethal bacterial strain in septic mice through an integrative approach.

Signal Transduct Target Ther 2022 07 25;7(1):245. Epub 2022 Jul 25.

Guangdong Provincial Key Laboratory of Proteomics, State Key Laboratory of Organ Failure Research, Department of Pathophysiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.

Effectively killing pathogenic bacteria is key for the treatment of sepsis. Although various anti-infective drugs have been used for the treatment of sepsis, the therapeutic effect is largely limited by the lack of a specific bacterium-targeting delivery system. This study aimed to develop antibacterial peptides that specifically target pathogenic bacteria for the treatment of sepsis. The lethal bacterial strain Escherichia coli MSI001 was isolated from mice of a cecal ligation and puncture (CLP) model and was used as a target to screen bacterial binding heptapeptides through an integrative bioinformatics approach based on phage display technology and high-throughput sequencing (HTS). Heptapeptides binding to E. coli MSI001 with high affinity were acquired after normalization by the heptapeptide frequency of the library. A representative heptapeptide VTKLGSL (VTK) was selected for fusion with the antibacterial peptide LL-37 to construct the specific-targeting antibacterial peptide VTK-LL37. We found that, in comparison with LL37, VTK-LL37 showed prominent bacteriostatic activity and an inhibitive effect on biofilm formation in vitro. In vivo experiments demonstrated that VTK-LL37 significantly inhibited bacterial growth, reduced HMGB1 expression, alleviated lesions of vital organs and improved the survival of mice subjected to CLP modeling. Furthermore, membrane DEGP and DEGQ were identified as VTK-binding proteins by proteomic methods. This study provides a novel strategy for targeted pathogen killing, which is helpful for the treatment of sepsis in the era of precise medicine.
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http://dx.doi.org/10.1038/s41392-022-01035-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309159PMC
July 2022

Identifying neuroimaging biomarkers for psychogenic erectile dysfunction by fusing multi-level brain information: A resting-state functional magnetic resonance imaging study.

Andrology 2022 Jul 23. Epub 2022 Jul 23.

Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, China.

Background: Psychogenic erectile dysfunction (pED) patients who are under their 40s in China consist of a major component of erectile dysfunction. Existing neuroimaging studies have demonstrated that pED is a functional disorder with aberrant neural representations on the local level, the regional level, and the global level, respectively. Therefore, it is reasonable to incorporate brain information from all these levels simultaneously into consideration when identifying neuroimaging biomarkers for pED. However, no such endeavors have been made in previous studies to fully disclose the central mechanism of pED.

Method: To incorporate multi-level brain features to fully explore the neural representation of pED, a novel machine learning framework was proposed in the current study. Specifically, we used amplitude of low-frequency fluctuation, regional homogeneity, and degree centrality as indices for local, regional, and global brain activity, respectively. A fully data-driven method, that is, support vector machine (SVM) with recursive feature elimination analyses, was used to investigate discriminative brain map between 48 pED patients and 39 healthy control subjects for resting state functional magnetic resonance imaging (rs-fMRI) data.

Results: By fusing multi-level brain features, our method led to a superb classification accuracy of 95.12% between two groups. Interestingly, the right anterior cingulate gyrus and the left precuneus showed abnormal representations at different levels simultaneously in pED patients, which also explicated highest discriminative power between groups. Moreover, the right insular, the left fusiform gyrus, the right inferior temporal gyrus, the right superior frontal gyrus, the right precentral gyrus, the bilateral parahippocampal gyrus, and the bilateral inferior frontal gyrus were discriminative for pED. Also, correlation analysis explicated that several core brain regions were associated with the clinical manifestations in pED patients.

Conclusion: This is one of the first study investigating brain alterations on different levels simultaneously in pED patients. Our results suggested that pED involves multi-level aberrant brain representations in multi-dimensional neurobehavioral components, which closely interrelated with cognitive and psychosocial factors, that is, attention, appraisal, emotion, and sensorimotor. Our findings are likely to help foster new insights into the pathophysiological mechanisms of pED and the aberrant brain regions may serve as potential therapeutic targets for targeted therapy for brain.
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http://dx.doi.org/10.1111/andr.13238DOI Listing
July 2022

Preliminary study on the role and mechanism of KIRREL3 in the development of esophageal squamous cell carcinoma.

Pathol Res Pract 2022 Jul 14;237:154025. Epub 2022 Jul 14.

The Pathophysiology Department, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Provincial Cooperative Innovation Center for Cancer Chemoprevention, Zhengzhou University, Zhengzhou 450001, China; Cancer Chemoprevention International Collaboration Laboratory, Zhengzhou 450001, China. Electronic address:

Background: Esophageal squamous cell carcinoma (ESCC) is a common malignant tumor of the digestive tract, which is very harmful to human health. The JAK-STAT signaling pathway is a recognized carcinogenic pathway that plays a role in the proliferation, apoptosis, migration, and invasion of a variety of cancer cells. Some studies have shown that the activation status of STAT3 affects the expression of KIRREL3. However, the expression of KIRREL3 in ESCC and its relationship with KIRREL3 or the JAK-STAT signaling pathway is still unclear.

Methods: In this study, we used immunohistochemistry and western blotting to analyze the protein expression levels of KIRREL3 in tumor tissues and ESCC cell lines. We applied proliferation assays, plate clone formation assays, Transwell assays, flow cytometry analysis, and CDX animal models to examine the role of KIRREL3 in ESCC.

Results: The results indicate that KIRREL3 is highly expressed to varying degrees in ESCC tissues and cell lines. Knocking down KIRREL3 expression in ESCC cells could correspondingly inhibit cell proliferation, colony formation, invasion, and migration, and had some effects on cell cycle progression and apoptosis. In addition, overexpressing KIRREL3 in these cells had opposite effects. Tumor formation in nude mice experiments also confirmed that KIRREL3 is involved in the growth of ESCC cells in vivo.

Conclusions: These data suggest that KIRREL3 plays a key role in the development of ESCC, and KIRREL3 is a potential new target for the early diagnosis and clinical treatment of this disease.
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http://dx.doi.org/10.1016/j.prp.2022.154025DOI Listing
July 2022

Genome Sequence of Acinetobacter baumannii Strain SHOU-Ab01, Isolated from Chinese Giant Salamander (Andrias davidianus) Liver.

Microbiol Resour Announc 2022 Jul 12:e0050322. Epub 2022 Jul 12.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, China.

This report describes the complete genome sequence of Acinetobacter baumannii strain SHOU-Ab01, which was isolated from the liver of a Chinese giant salamander (Andrias davidianus). SHOU-Ab01 belonged to sequence type 40 (ST40), and its genome contained a circular chromosome (size, 3,891,862 bp) and two circular plasmids (sizes, 8,571 bp and 5,870 bp).
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http://dx.doi.org/10.1128/mra.00503-22DOI Listing
July 2022

Angiotensin-Converting Enzyme 2 Potentiates SARS-CoV-2 Infection by Antagonizing Type I Interferon Induction and Its Down-Stream Signaling Pathway.

mSphere 2022 Jul 12:e0021122. Epub 2022 Jul 12.

Clinical Center for Bio-Therapy, Zhongshan Hospital, Fudan University (Xiamen Branch), Shanghai, China.

The innate interferon (IFN) response constitutes the first line of host defense against viral infections. It has been shown that IFN-I/III treatment could effectively contain severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication . However, how SARS-CoV-2 survives through the innate antiviral mechanism remains to be explored. Our study uncovered that human angiotensin-converting enzyme 2 (ACE2), identified as a primary receptor for SARS-CoV-2 entry, can disturb the IFN-I signaling pathway during SARS-CoV-2 infection in human lung cells. We identified that ACE2 was significantly upregulated by SARS-CoV-2 and Sendai virus (SeV) infection, and exogenous expression of ACE2 suppressed IFN-I production in a dose-dependent manner. Mechanistically, ACE2 disrupted poly (I:C)-mediated inhibition of SARS-CoV2 replication by antagonizing IFN-I production by blocking IRF3 phosphorylation and nuclear translocation. Moreover, ACE2 quenched the IFN-mediated antiviral immune response by degrading endogenous STAT2 protein, inhibiting STAT2 phosphorylation and nuclear translocation. Interestingly, IFN-inducible short ACE2 (dACE2 or MIRb-ACE2) can also be induced by virus infection and inhibits the IFN signaling. Thus, our findings provide mechanistic insight into the distinctive role of ACE2 in promoting SARS-CoV-2 infection and enlighten us that the development of interventional strategies might be further optimized to interrupt ACE2-mediated suppression of IFN-I and its signaling pathway. Efficient antiviral immune responses against SARS-CoV-2 infection play a key role in controlling the coronavirus diseases 2019 (COVID-19) caused by this virus. Although SARS-CoV-2 has developed strategies to counteract the IFN-I signaling through the virus-derived proteins, our knowledge of how SARS-CoV-2 survives through the innate antiviral mechanism remains poor. We herein discovered the distinctive role of ACE2 as a restraining factor of the IFN-I signaling in facilitating SARS-CoV-2 infection in human lung cells. Both full-length ACE2 and truncated dACE2 can antagonize IFN-mediated antiviral response. These findings are key to understanding the counteraction between SARS-CoV-2 pathogenicity and the host antiviral defenses.
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http://dx.doi.org/10.1128/msphere.00211-22DOI Listing
July 2022

Contributes to Anthocyanin Sequestration in Radish ( L.).

Front Plant Sci 2022 4;13:870202. Epub 2022 Jul 4.

College of Life Sciences, Nanjing Agricultural University, Nanjing, China.

Anthocyanins are water-soluble plant pigments mainly stored in the plant vacuoles. Glutathione S-transferases (GSTs) are a multifunctional enzyme family, which can regulate substance metabolism and biological and abiotic stresses in plants. However, few reports were focused on the involvement of GSTs in anthocyanin sequestration in red skin radish. Here, we identified a glutathione S-transferase gene that played roles in anthocyanin sequestration in radish. The bioinformatics analysis revealed that RsGSTF12 belonged to the phi (F) class of glutathione S-transferases and showed a high homology with AtGSTF12, followed by AtGSTF11. The subcellular localization assay showed that RsGSTF12 was located in the endoplasmic reticulum and tonoplast. Temporal and spatial gene expression-specific analyses uncovered a strong correlation of with anthocyanin accumulation in radish sprouts. The anthocyanin solubility assay found RsGSTF12 was capable of improving cyanidin water solubility . Transiently expressing in radish cotyledons was able to increase their anthocyanin sequestrations. Furthermore, the functional complementation and overexpression of the mutant and wild type demonstrated that might play an indispensable role in anthocyanin accumulation in radish. Taken together, we provide compelling evidence that RsGSTF12 functions critically in how anthocyanins are sequestrated in radish, which may enrich our understanding of the mechanism of anthocyanin sequestration.
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http://dx.doi.org/10.3389/fpls.2022.870202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289562PMC
July 2022

Widely targeted metabolomics analysis characterizes the phenolic compounds profiles in mung bean sprouts under sucrose treatment.

Food Chem 2022 Nov 30;395:133601. Epub 2022 Jun 30.

Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, China; College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, China.

Phenolic compounds are one of the wholesome substances of mung bean sprouts, showing numerous health-promoting functions. Here, effects of sucrose on phenolic compounds profiles of mung bean sprouts were investigated. Results showed that the content and composition of phenolic compounds were significantly altered by 1‰ and 5‰ sucrose, respectively. The antioxidant capacity was significantly improved by sucrose. Based on metabolomics, 251 metabolites were detected, of which 106 were phenolic compounds. Correlation analysis showed 21 phenolics were positively correlated with antioxidant capacity. The changes in phenolic composition and antioxidant capacity after sucrose treatment were mainly due to the enrichment of phenolic biosynthesis pathways. Moreover, the gene expression and enzyme activity analysis of key phenolic biosynthetic genes contributed to elucidate the phenolic profile under sucrose treatment. In summary, mung bean sprouts are promising sources of dietary phenolic compounds and sucrose treatment is a good process to produce phenolic-rich mung bean sprouts.
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http://dx.doi.org/10.1016/j.foodchem.2022.133601DOI Listing
November 2022

Allocation of Flood Drainage Rights in Watershed Using a Hybrid FBWM-Grey-TOPSIS Method: A Case Study of the Jiangsu Section of the Sunan Canal, China.

Int J Environ Res Public Health 2022 07 4;19(13). Epub 2022 Jul 4.

College of Civil Engineering, Nanjing Forestry University, Nanjing 210037, China.

In this study, an FDR allocation scheme based on synergetic theory was designed to alleviate the drainage conflicts caused by the grabbing of flood drainage rights (FDR) in each region of the basin. An FDR allocation index system was constructed by employing synergetic theory and following the principles of safety, equity, efficiency, and sustainability. A new multi-criteria decision-making method, called FBWM-Grey-TOPSIS, was developed, which is based on the integration of the fuzzy best-worst method (FBWM) and Grey-TOPSIS. Among them, the FBWM method was used to distinguish the importance of subsystems and order parameters, and the Grey-TOPSIS method is applied to obtain the optimal FDR assignment results. Taking the Jiangsu section of the Sunan Canal as an example, the FDRs of the four regions in the basin were allocated. The results reveal that the proportion of FDRs obtained in descending order is Changzhou (32.69%), Suzhou (24.88%), Wuxi (23.01%), and Zhenjiang (19.42%). In addition, the performance of the proposed method is demonstrated by sensitivity analysis and comparative analysis with the existing methods. The methodology and research results presented in this paper can help governments and agencies achieve a scientific allocation of FDR in watersheds, thus promoting harmonious watershed development.
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http://dx.doi.org/10.3390/ijerph19138180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266102PMC
July 2022

Comprehensive RNA-seq reveals molecular changes in kidney malignancy among people living with HIV.

Mol Ther Nucleic Acids 2022 Sep 14;29:91-101. Epub 2022 Jun 14.

Shanghai Public Health Clinical Center, Fudan University, 2901 Caolang Road, Jinshan District, Shanghai 201508, China.

To heighten the awareness of kidney malignancy in patients with HIV infection to facilitate the early diagnosis of kidney cancer, the differentially expressed mRNAs were analyzed in this malignant tumor using RNA sequencing. We identified 2,962 protein-coding transcripts in HIV-associated kidney cancer. KISS1R, CAIX, and NPTX2 mRNA expression levels were specifically increased in HIV-associated kidney cancer while UMOD and TMEM213 mRNA were decreased in most cases based on real-time PCR analyses. These findings were similar to those noted for the general population with renal cell carcinoma. Immunohistochemical staining analysis also showed that a total of 18 malignant kidney cases among the people living with HIV (PLWH) exhibited positive staining for KISS1R and CAIX. Pathway analysis of the differentially expressed mRNAs in HIV-associated kidney cancer revealed that several key pathways were involved, including vascular endothelial growth factor-activated receptor activity, IgG binding, and lipopolysaccharide receptor activity. Altogether, our findings reveal the identified molecular changes in kidney malignancy, which may offer a helpful explanation for cancer progression and open up new therapeutic avenues that may decrease mortality after a cancer diagnosis among PLWH.
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http://dx.doi.org/10.1016/j.omtn.2022.06.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240952PMC
September 2022

Identification of Key Biomarkers and Immune Infiltration in Liver Tissue after Bariatric Surgery.

Dis Markers 2022 25;2022:4369329. Epub 2022 Jun 25.

Department of Endocrinology and Metabolism, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Background: Few drugs are clearly available for nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH); nevertheless, mounting studies have provided sufficient evidence that bariatric surgery is efficient for multiple metabolic diseases, including NAFLD and NASH, while the molecular mechanisms are still poorly understood.

Methods: The mRNA expression profiling of GSE48452 and GSE83452 were retrieved and obtained from the Gene Expression Omnibus (GEO) database. The limma package was employed for identifying differentially expressed genes (DEGs), followed by clusterProfiler for performing Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, and GSEA software for performing GSEA analyses. The PPI network analyses were constructed using Metascape online analyses. WGCNA was also utilized to identify and verify the hub genes. CIBERSORT tools contributed to the analysis of immune cell infiltration of liver diseases.

Results: We identify coexpressed differential genes including 10 upregulated and 55 downregulated genes in liver tissue after bariatric surgery. GO and KEGG enrichment analyses indicated that DEGs were remarkably involved in the immune response. GSEA demonstrated that DEGs were markedly enriched in the immune response before surgery, while most were enriched in metabolism after surgery. Seven genes were screened through the MCC algorithm and KME values, including SRGN, CD53, EVI2B, MPEG1, NCKAP1L, LCP1, and TYROBP. The mRNA levels of these genes were verified in the Attie Lab Diabetes Database, and only LCP1 was found to have significant differences and correlation with certain immune cells.

Conclusion: Our knowledge of the mechanisms by which bariatric surgery benefits the liver and the discovery of LCP1 is expected to serve as potential biomarkers or therapeutic targets for NAFLD and NASH.
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http://dx.doi.org/10.1155/2022/4369329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250435PMC
July 2022

FRZB is Regulated by the Transcription Factor EGR1 and Inhibits the Growth and Invasion of Triple-Negative Breast Cancer Cells by Regulating the JAK/STAT3 Pathway.

Clin Breast Cancer 2022 Jun 2. Epub 2022 Jun 2.

Background: To explore the expression of frizzled related protein (FRZB) in triple-negative breast cancer (TNBC) and role of FRZB in TNBC cell growth and invasion.

Methods: Breast cancer clinical data were downloaded from the Cancer Genome Atlas. FRZB and early growth response 1 (EGR1) mRNA levels in TNBC were measured by quantitative real-time polymerase chain reaction. FRZB protein level was measured by immunohistochemistry and western blot. Proliferation, migration, and invasion of TNBC cells were detected by colony formation, wound healing, and transwell assay, respectively. The protein levels of EGR1, E-cadherin, N-cadherin, Snail, p-JAK1/JAK1, p-JAK2/JAK2, and p-STAT3/STAT3 were measured by western blot. JASPAR was used to predict the binding site of FRZB and EGR1. The binding ability of FRZB and EGR1 was verified by dual-luciferase reporter gene assay and chromatin immunoprecipitation assay.

Results: FRZB was low expressed in TNBC tissues and cells. Silencing FRZB promoted cell proliferation, migration, invasion, and EMT and activated JAK/STAT pathway in MDA-MB-468 and MDA-MB-231 cells, but overexpression of FRZB acted opposite effects in MDA-MB-468 and MDA-MB-231 cells. EGR1 was low expressed in TNBC samples and positively correlated with FRZB. Moreover, EGR1 could recover the promotion of silencing FRZB on cell proliferation, migration, invasion, and JAK/STAT pathway in MDA-MB-468 cells, but silencing EGR1 led to the opposite results in MDA-MB-231 cells.

Conclusion: FRZB was low expressed in TNBC and was regulated by EGR1, and FRZB inhibited TNBC cell growth and invasion by regulating the JAK/STAT3 pathway.
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http://dx.doi.org/10.1016/j.clbc.2022.05.010DOI Listing
June 2022

Single-cell sequencing reveals CD133CD44-originating evolution and novel stemness related variants in human colorectal cancer.

EBioMedicine 2022 Jul 1;82:104125. Epub 2022 Jul 1.

Department of Radiotherapy, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address:

Background: Tumor heterogeneity of human colorectal cancer (CRC)-initiating cells (CRCICs) in cancer tissues often represents aggressive features of cancer progression. For high-resolution examination of CRCICs, we performed single-cell whole-exome sequencing (scWES) and bulk cell targeted exome sequencing (TES) of CRCICs to investigate stemness-specific somatic alterations or clonal evolution.

Methods: Single cells of three subpopulations of CRCICs (CD133CD44, CD133CD44, and CD133CD44 cells), CRC cells (CRCCs), and control cells from one CRC tissue were sorted for scWES. Then, we set up a mutation panel from scWES data and TES was used to validate mutation distribution and clonal evolution in additional 96 samples (20 patients) those were also sorted into the same three groups of CRCICs and CRCCs. The knock-down experiments were used to analyze stemness-related mutant genes. Neoantigens of these mutant genes and their MHC binding affinity were also analyzed.

Findings: Clonal evolution analysis of scWES and TES showed that the CD133CD44 CRCICs were the likely origin of CRC before evolving into other groups of CRCICs/CRCCs. We revealed that AHNAK2, PLIN4, HLA-B, ALK, CCDC92 and ALMS1 genes were specifically mutated in CRCICs followed by the validation of their functions. Furthermore, four predicted neoantigens of AHNAK2 were identified and validated, which might have applications in immunotherapy for CRC patients.

Interpretation: All the integrative analyses above revealed clonal evolution of CRC and new markers for CRCICs and demonstrate the important roles of CRCICs in tumorigenesis and progression of CRCs.

Funding: A full list of funding bodies that contributed to this study can be found in the Acknowledgements section.
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http://dx.doi.org/10.1016/j.ebiom.2022.104125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254347PMC
July 2022

First morning voided urinary gonadotropins in children: verification of method performance and establishment of reference intervals.

Clin Chem Lab Med 2022 08 4;60(9):1416-1425. Epub 2022 Jul 4.

Department of Pediatrics, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, P.R. China.

Objectives: Urinary luteinizing hormone (uLH) and urinary follicle-stimulating hormone (uFSH) have been shown to be useful screening and management tools for children with central precocious puberty. However, studies on uLH and uFSH reference intervals are scarce. Therefore, we aimed to establish reference intervals for uLH and uFSH, according to age, sex, and pubertal status in apparently healthy children aged 6-11 years.

Methods: We performed detection capability, precision, accuracy by recovery, linearity, agreement analysis, and stability testing to analyze the method performance of uLH and uFSH. The Clinical Laboratory Standards Institute's C28-A3 criteria was used to establish the reference intervals.

Results: Both uLH and uFSH were stable at 4 °C for 52.6 h and 64.8 days, respectively. The total imprecision of uFSH is within the manufacturer's claim, while the total imprecision of uLH remained within tolerable bias. Both uLH and uFSH could be measured with acceptable detection capability. The recovery rates of the hormones were 87.6-98.8% and 102.8-103.4%, respectively, and therefore within acceptable limits. There were significant correlations between the serum and urine concentrations (LH: r=0.91, p<0.001; FSH: r=0.90, p<0.001). The reference intervals of uLH and uFSH were established according to age, sex, and pubertal status.

Conclusions: We established reference intervals for uLH and uFSH based on age, sex and pubertal status to provide a non-invasive clinical screening tool for precocious puberty in children aged 6-11 years.
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http://dx.doi.org/10.1515/cclm-2022-0296DOI Listing
August 2022

Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2.

Nat Microbiol 2022 07 30;7(7):1063-1074. Epub 2022 Jun 30.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Institute of Infectious Diseases and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.

Frequent outbreaks of coronaviruses underscore the need for antivirals and vaccines that can counter a broad range of coronavirus types. We isolated a human antibody named 76E1 from a COVID-19 convalescent patient, and report that it has broad-range neutralizing activity against multiple α- and β-coronaviruses, including the SARS-CoV-2 variants. 76E1 also binds its epitope in peptides from γ- and δ-coronaviruses. 76E1 cross-protects against SARS-CoV-2 and HCoV-OC43 infection in both prophylactic and therapeutic murine animal models. Structural and functional studies revealed that 76E1 targets a unique epitope within the spike protein that comprises the highly conserved S2' site and the fusion peptide. The epitope that 76E1 binds is partially buried in the structure of the SARS-CoV-2 spike trimer in the prefusion state, but is exposed when the spike protein binds to ACE2. This observation suggests that 76E1 binds to the epitope at an intermediate state of the spike trimer during the transition from the prefusion to the postfusion state, thereby blocking membrane fusion and viral entry. We hope that the identification of this crucial epitope, which can be recognized by 76E1, will guide epitope-based design of next-generation pan-coronavirus vaccines and antivirals.
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http://dx.doi.org/10.1038/s41564-022-01155-3DOI Listing
July 2022

Cystatin C-based estimated GFR performs best in identifying individuals with poorer survival in an unselected Chinese population: results from the China Health and Retirement Longitudinal Study (CHARLS).

Clin Kidney J 2022 Jul 7;15(7):1322-1332. Epub 2022 Mar 7.

Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: The decline in estimated glomerular filtration rate (eGFR) has been reported as a risk factor for mortality. However, it remains unclear which eGFR equation is most useful in predicting death in the general Chinese population.

Methods: The association was examined between eGFR and all-cause mortality using data from the China Health and Retirement Longitudinal Study. Participants with complete data in 2011 and survival follow-up in 2013, 2015 and 2018 were included and analyzed in three separate cohorts, which included 8160, 8154 and 8020 participants, respectively. Logistic regression analyses, receiver operating characteristic curve, continuous net reclassification improvement (NRI) and integrated discrimination improvement (IDI) were computed to compare the discriminative power of eGFR derived by abbreviated Modification of Diet in Renal Disease (MDRD), Chinese coefficient-modified MDRD (MDRD-CN), Japanese coefficient-modified MDRD (MDRD-JPN), CKD-EPI, Japanese coefficient-modified CKD-EPI (CKD-EPI-JPN), CKD-EPI, CKD-EPI, CKD-EPI fit without race and CKD-EPI fit without race.

Results: A decreased eGFR (<60 ml/min/1.73 m) was significantly associated with increased mortality at 2 years no matter which eGFR equation was used (odds ratio ranged between 2.02 and 4.94, all P < 0.001). The association remained significant after adjusting multiple covariates when MDRD-CN, CKD-EPI or CKD-EPI fit without race was used. CKD-EPI showed the highest discriminative power for mortality (area under the curve 0.744 ± 0.40) and outperformed other equations (all P < 0.001) except for CKD-EPI. The overall risk classification was also improved when the CKD-EPI equation was adopted as indicated by continuous NRI and IDI. Similar results were observed at 4 and 7 years.

Conclusions: A decline in eGFR by all equations could predict poorer survival, among which the CKD-EPI equation showed the best discriminative power.
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http://dx.doi.org/10.1093/ckj/sfac070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217656PMC
July 2022
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