Publications by authors named "Xiaoyan Han"

133 Publications

Risk factors of intraocular lens dislocation following routine cataract surgery: a case-control study.

Clin Exp Optom 2021 Feb 10:1-8. Epub 2021 Feb 10.

Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai, China.

: Intraocular lens dislocation usually causes severe visual symptoms and even blindness in patients. It is important for eye care practitioners to be able to identify patients susceptible to intraocular lens dislocation. : To investigate the risk factors of intraocular lens dislocation following routine cataract surgery. : This retrospective case-control study included patients diagnosed with intraocular lens dislocation between January 2007 and March 2018. Controls matched to the cases by age and time of routine cataract surgery in a ratio of 3:1 were randomly selected from patients without intraocular lens dislocation. The medical records of the patients were reviewed and analysed. Independent -test, chi-square test, and logistic regression analysis were employed to identify risk factors of intraocular lens dislocation. : In total, 127 cases (with intraocular lens dislocation) and 381 controls (without intraocular lens dislocation) were evaluated. Among cases, the proportion of men was significantly higher than that of women (p = 0.012). High myopia was significantly associated with all types of intraocular lens dislocation; it was also a risk factor for in-the-bag intraocular lens dislocation. Lens subluxation was significantly associated with the in-the-bag and late intraocular lens dislocations. Intraoperative and post-operative vitrectomy, rather than pre-operative vitrectomy, were associated with intraocular lens dislocation. Post-operative trauma was significantly associated with in-the-bag intraocular lens dislocation; pre-operative and post-operative trauma were significantly associated with out-of-the-bag intraocular lens dislocation. Male sex (odds ratio (OR) = 2.996, p = 0.006), pre-operative trauma (OR = 7.861, p = 0.014), high myopia (OR = 11.268, p < 0.001), and lens subluxation (OR = 13.431, p = 0.018) were the risk factors of intraocular lens dislocation. : Male sex, pre-operative trauma, high myopia, and lens subluxation were the main predisposing factors for intraocular lens dislocation in Chinese Han population.
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http://dx.doi.org/10.1080/08164622.2021.1878829DOI Listing
February 2021

Successful endoscopic management of 3 cases of translocated intrauterine devices: a case report.

Ann Palliat Med 2021 Feb 18;10(2):2371-2378. Epub 2021 Feb 18.

Department of Gynecology and Obstetrics, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Intrauterine devices (IUDs) are the most popular form of contraception globally; however, the use of IUDs carries some risks. IUD migration is a rare but serious complication that occurs after insertion, sometimes years later. Herein, we report 3 cases of translocated IUDs removed by endoscopy and describe the clinical characteristics and intraoperative findings of these patients. Case 1 was a 46-year-old woman who presented with upper abdominal pain. Gastroscopy showed the presence of an IUD inside the stomach. Digestive endoscopy performed at our hospital showed a T-shaped foreign body embedded in the gastric wall at the junction between the gastric antrum and the gastric body. The IUD was removed endoscopically without complications. Case 2 was a 36-year-old woman who had twice undergone cesarean section. Two months before presenting at our hospital, the patient experienced external hemorrhoid. During anal suppository treatment, she felt a lump inside the rectum. Pelvic computed tomography revealed a small, circular dense shadow with an approximate diameter of 0.4 cm in the upper intestinal cavity of the rectum. Subsequently, the patient received transanal endoscopy and rectal foreign-body removal surgery. Case 3 was a 40-year-old woman. She had failed to remove an IUD in other 2 hospitals. On the basis of pelvic computed tomography, the IUD was suspected to have perforated the bladder. Cystoscopy showed that part of the IUD was surrounded by stones. Laser lithotripsy was performed to expose the IUD completely. Under hysteroscopy, the IUD could be seen in the middle of the uterine cavity; 1 end was located on the right uterine horn, and the other was protruding out of the uterus. During the operation, the IUD was broken into two parts and one part was removed through the vagina. The cystoscope was placed again, and the remaining part of the IUD was smoothly removed. Although IUD migration has a low incidence, it can have a huge impact on patients' physical and emotional health. Migrated IUDs must be removed immediately. Hysteroscopy and digestive endoscopy are effective approaches for removing migrated IUDs.
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http://dx.doi.org/10.21037/apm-21-184DOI Listing
February 2021

Decoding the Complex Free Radical Cascade by Using a DNA Framework-Based Artificial DNA Encoder.

Angew Chem Int Ed Engl 2021 Feb 8. Epub 2021 Feb 8.

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China.

DNA-based molecular communications (DMC) are critical for regulating biological networks to maintain stable organismic functions. However, the complicated, time-consuming information transmission process involved in genome-coded DMC and the limited, vulnerable decoding activity generally lead to communication impairment or failure, in response to external stimuli. Herein, we present a conceptually innovative DMC strategy mediated by the DNA framework-based artificial DNA encoder. With the free-radical cascade as a proof-of-concept study, the artificial DNA encoder shows active sensing and real-time actuation, in situ and broad free radical-decoding efficacy, as well as robust resistance to environmental noise. It can also block undesirable short-to-medium-range communications between free radicals and inflammatory networks, leading to a synergistic anti-obesity effect. The artificial DNA encoder-based DMC may be generalized to other communication systems for a variety of applications.
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http://dx.doi.org/10.1002/anie.202014088DOI Listing
February 2021

Elevated lipoprotein(a) and risk of coronary heart disease according to different lipid profiles in the general Chinese community population: the CHCN-BTH study.

Ann Transl Med 2021 Jan;9(1):26

Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University and Beijing Municipal Key Laboratory of Clinical Epidemiology, Beijing, China.

Background: To evaluate the contributions of elevated lipoprotein(a) [Lp(a)] to the risk of coronary heart disease (CHD) in the general Chinese community population according to different lipid profiles.

Methods: We recruited individuals aged over 18 years from the baseline survey of the Cohort Study on Chronic Disease of Communities Natural Population in Beijing, Tianjin and Hebei (CHCN-BTH) using a stratified, multistage cluster sampling method. Data were collected through questionnaire surveys, anthropometric measures and laboratory tests. Restricted cubic spline (RCS) functions, multivariate logistic regression, sensitivity analyses and stratified analyses were used to evaluate the association between Lp(a) and CHD.

Results: A total of 25,343 participants were included, with 1,364 (5.38%) identified as having CHD. Elevated Lp(a) levels were linearly related to an increased risk of CHD (P<0.0001 and P=0.8468). Multivariate logistic regression analysis indicated that subjects with Lp(a) ≥300 mg/L had a higher risk of CHD [OR (95% CI): 1.36 (1.17, 1.57)] than did individuals with Lp(a) <300 mg/L. Compared with individuals with Lp(a) <119.0 mg/L (<50th percentile), the ORs (95% CI) for CHD in the 51st-80th, 81st-95th and >95th percentiles were 1.07 (0.93, 1.23), 1.26 (1.07, 1.50) and 1.68 (1.30, 2.17), respectively (P for trend <0.0001). This association was also found among the subgroup of subjects without dyslipidemia, including those with normal total cholesterol (TC) (<6.2 mmol/L), triglycerides (TG) (<2.3 mmol/L), high-density lipoprotein cholesterol (HDL-C) (≥1.0 mmol/L) and low-density lipoprotein cholesterol (LDL-C) (<4.1 mmol/L). Elevated Lp(a) and dyslipidemia significantly contributed to a higher risk of CHD with synergistic effects. Stratified analyses showed that elevated Lp(a) concentrations were significantly associated with an increased risk of CHD in the subgroups of individuals who were noncurrent drinkers, overweight individuals, individuals with hypertension, individuals who engaged in moderate physical activity, those without diabetes mellitus and individuals in Beijing and Tianjin.

Conclusions: Elevated Lp(a) concentrations were linearly associated with a higher risk of CHD in the general Chinese community population, especially in normolipidemic subjects. Both dyslipidemia and elevated Lp(a) independently or synergistically contributed to the risk of CHD. Our results suggest that more attention should be paid to the levels of Lp(a) in normolipidemic subjects, which may be an early predictor of CHD.
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http://dx.doi.org/10.21037/atm-20-3899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859748PMC
January 2021

Toric Intraocular Lens Implantation in the Correction of Moderate-To-High Corneal Astigmatism in Cataract Patients: Clinical Efficacy and Safety.

J Ophthalmol 2021 20;2021:5960328. Epub 2021 Jan 20.

Eye Institute, Eye and Ear, Nose, and Throat, Hospital of Fudan University, 83 Fenyang Road, Shanghai 200031, China.

Methods: A total of 57 cataract patients (57 eyes) with regular corneal astigmatism (≥2.57 D) were enrolled in this retrospective cohort study. Phacoemulsification with toric IOL implantation was performed for all patients. The uncorrected visual acuity (UCVA) and best corrected visual acuity (BCVA) were recorded before and one year after surgery, and statistical analysis of preoperative corneal astigmatism, postoperative residual astigmatism, aberrations, IOL rotation, and related factors was performed to evaluate the efficacy, safety, and stability of toric IOLs in correcting moderate-to-high corneal astigmatism.

Results: One year after surgery, visual acuity was significantly improved compared with that before surgery (preoperative log MAR 0.87 ± 0.34 vs. postoperative log MAR 0.31 ± 0.26, < 0.001), and the self-reported spectacle independence rate was 68.42%. The total residual astigmatism was 1.18 ± 0.85 D, which was significantly less than the preoperative value (3.41 ± 0.99 D) ( < 0.001). The degree of toric IOL rotation was 4.93 ± 3.02°, and 54.39% of patients had a lens rotation of less than 5°. The IOLs of 5.26% (3 eyes) of patients rotated more than 10°, and these patients received glasses instead of undergoing IOL repositioning.

Conclusions: Toric IOL implantation provided optimal vision outcomes and low spectacle dependence during a one-year follow-up period. The results from our study show that toric IOL implantation is a safe and effective option for cataract patients with moderate-to-high corneal astigmatism.
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http://dx.doi.org/10.1155/2021/5960328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840247PMC
January 2021

Potentilla anserina L. developmental changes affect the rhizosphere prokaryotic community.

Sci Rep 2021 Feb 2;11(1):2838. Epub 2021 Feb 2.

School of Ecology, Environment and Resources, Qinghai Nationalities University, Bayi Road, Xining, 810007, Qinghai, China.

Plant roots and soil prokaryotes primarily interact with each other in the rhizosphere. Changes in the rhizosphere prokaryotic structure are influenced by several factors. In this study, the community structure of the Potentilla anserina L. rhizosphere prokaryotes was identified and evaluated by high-throughput sequencing technology in different continuous cropping fields and developmental stages of the plant. In total, 2 archaeal (Euryarchaeota and Thaumarchaeota) and 26 bacterial phyla were identified in the P. anserina rhizosphere. The bacterial community was mainly composed of Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Gemmatimonadetes, Planctomycetes, Proteobacteria, and Verrucomicrobia. Moreover, the prokaryotic community structure of the rhizosphere varied significantly during plant development. Our results provide new insights into the dynamics of the P. anserina rhizosphere prokaryotic community and may provide useful information for enhancing the growth and development of P. anserina through artificial control of the soil prokaryotes.
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http://dx.doi.org/10.1038/s41598-021-82610-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854623PMC
February 2021

Identification of Specific Glycosyltransferases Involved in Flavonol Glucoside Biosynthesis in Ginseng Using Integrative Metabolite Profiles, DIA Proteomics, and Phylogenetic Analysis.

J Agric Food Chem 2021 Feb 29;69(5):1714-1726. Epub 2021 Jan 29.

Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Ginseng contains a variety of flavonol glycosides that possess diverse biological activities; however, scant information of flavonoid glycosylation was reported in ginseng. We found that panasenoside and kaempferol 3--glucoside were commonly accumulated along with cultivation years in leaves. In order to explore the procedure of flavonol glycosylation in ginseng, 50 UDP-glycosyltransferases (UGTs) were screened out using differentiated data-independent acquisition (DIA) proteomics and phylogenetic analysis. UGT92A10 and UGT94Q4 were found contributing to the formation of kaempferol 3--glucoside. UGT73A18, UGT74T4, and UGT75W1 could catalyze galactosylation of kaempferol 3--glucoside. Ser278, Trp335, Gln338, and Val339 were found forming hydrogen bonds with UDP-galactose in UGT75W1 by docking. MeJA induced transcripts of UGT73A18 and UGT74T4 by over fourfold, consistent with the decrease of kaempferol 3--glucoside, which indicated that these genes may be related to resisting adversity stress in ginseng. These results highlight the significance of integrative metabolite profiles, proteomics, and phylogenetic analysis for exploring flavonol glycosylation in ginseng.
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http://dx.doi.org/10.1021/acs.jafc.0c06989DOI Listing
February 2021

Acute myeloid leukemia with CPSF6-RARG fusion resembling acute promyelocytic leukemia with extramedullary infiltration.

Ther Adv Hematol 2021 9;12:2040620720976984. Epub 2021 Jan 9.

Bone Marrow Transplantation Center, Department of Hematology, The First Affiliated Hospital, School of Medicine, Zhejiang University, No. 79, Qingchun Road, Hangzhou, Zhejiang Province 310003, China.

Some subtypes of acute myeloid leukemia (AML) share morphologic, immunophenotypic, and clinical features of acute promyelocytic leukemia (APL), but lack a (promyelocytic leukemia-retinoic acid receptor alpha) fusion gene. Instead, they have the retinoic acid receptor beta (RARB) or retinoic acid receptor gamma (RARG) rearranged. Almost all of these AML subtypes exhibit resistance to all-trans retinoic acid (ATRA); undoubtedly, the prognosis is poor. Here, we present an AML patient resembling APL with a novel cleavage and polyadenylation specific factor 6 () fusion, showing resistance to ATRA and poor response to chemotherapy with homoharringtonine and cytarabine. Simultaneously, the patient also had extramedullary infiltration.
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http://dx.doi.org/10.1177/2040620720976984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797573PMC
January 2021

Analysis of corneal astigmatism and aberration in chinese congenital cataract and developmental cataract patients before cataract surgery.

BMC Ophthalmol 2021 Jan 13;21(1):34. Epub 2021 Jan 13.

Eye Institute and Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 200031, Shanghai, China.

Background: To investigate the distribution of corneal astigmatism, aberration, and biometric parameters in Chinese congenital cataract (CC) /developmental cataract patients before cataract surgery.

Methods: We evaluated eyes of CC /developmental cataract patients scheduled for cataract surgery from January 2016 to September 2019. Astigmatism, aberrations, and biometric parameters were measured with the Pentacam Scheimpflug imaging device (Pentacam HR, Oculus). Cataract was diagnosed and classified by slit-lamp examination after full mydriasis.

Results: We evaluated 538 eyes in 356 patients. The mean values of anterior corneal astigmatism (ACA), posterior corneal astigmatism (PCA), and total corneal astigmatism (TCA) were determined as 1.98 ± 1.06 D (range 0.0‒4.8 D), 0.49 ± 0.26 D (range 0.0‒1.9 D), and 2.09 ± 1.19 D (range 0.2‒8.8 D), respectively. ACA and TCA ≥ 1.25 D was present in 379 eyes (70.3%) and 392 eyes (72.8%), respectively. PCA between 0.25 D and 0.75 D was found in 380 eyes (70.6%). There was a statistically significant positive linear correlation between lower-order aberrations root mean square values (LOA RMS) and corneal astigmatism (CA). Furthermore, in terms of distribution of central cornea thickness, anterior chamber depth, ACA, PCA, and TCA in different types of cataracts, ACA was highest in patients with zonular cataracts. Finally, we found anterior corneal measurements may overestimate WTR astigmatism, underestimate ATR astigmatism, and underestimate oblique astigmatism, respectively.

Conclusions: Most CC /developmental cataract patients had moderate to high astigmatism and ACA accounted for the largest proportion in the zonular group. This can provide a basis for planning of CC/developmental cataract surgery by ophthalmologists in clinical practice.
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http://dx.doi.org/10.1186/s12886-020-01794-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7805192PMC
January 2021

p. nov., a nicosulfuron degrading bacterium, isolated from a microbial consortium.

Int J Syst Evol Microbiol 2021 Feb;71(2)

College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, PR China.

A Gram-stain-negative, aerobic, motile, short-rod-shaped bacterium with nicosulfuron-degrading ability, designated strain LAM1902, was isolated from a microbial consortium enriched with nicosulfuron as a sole nitrogen and energy source. The optimal temperature and pH for growth of strain LAM1902 were 30 °C and pH 6.0, respectively. Strain LAM1902 could grow in the presence of NaCl with concentration up to 4.0 % (w/v). Comparative analysis of 16S rRNA gene sequences revealed that LAM1902 was closely related to the members of the family to the genus , with the highest similarity to DSM 14399 (99.6 %), WZBFD3-5A2 (99.3 %) and Esp-1 (98.8 %). Multi-locus sequence analysis based on both concatenated sequences of the 16S rRNA gene and three housekeeping genes (, and ) further confirmed the intrageneric phylogenetic position of strain LAM1902. The genomic DNA G+C content of LAM1902 was 64.8 mol%. The low values of DNA-DNA hybridization (less than 43.7 %) and average nucleotide identity (less than 90.9 %) also showed that the strain was distinctly different from known species of the genus . The major fatty acids were C, C cyclo and anteiso C. Ubiquinone Q-9 was detected as the predorminant respiratory quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on phylogenetic, phenotypic and chemotaxonomic analyses and genome comparisons, we conclude that strain LAM1902 represents a novel species, for which the name sp. nov. is proposed. The type strain is LAM1902 (=JCM 33860=KCTC 72830).
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http://dx.doi.org/10.1099/ijsem.0.004632DOI Listing
February 2021

Bortezomib-Based Regimens for Newly Diagnosed Multiple Myeloma in China: A Report of 12-Year Real-World Data.

Front Pharmacol 2020 11;11:561601. Epub 2020 Dec 11.

Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Improve the treatment quality might affect patients' efficacy and survival. Five hundred thirty multiple myeloma patients treated in four hematological centers in China from February 2006 to August 2018 were enrolled. General characteristics, treatment regimens and cycles, efficacy, survival and adverse events of the patients treated before and after August 2013 (later refer to as the before-2013 and after-2013 group) were analyzed and compared. The results suggested that patients who received optimized treatment regimen and route of administration completed more cycles of treatment in the after-2013 group. Although the overall response rate was similar between the two groups (88.6 vs. 90.5%), patients in the after-2013 group had higher complete remission rate (39.1 vs. 28.6%) and better progression-free survival. Subgroup analysis suggested that patients aged 65 years and older, with non-high-risk D-S, ISS, and R-ISS stages, had a significant benefit in progression-free survival. Therefore, in clinical practice in China, by reducing the economic burden brought by the treatment on patients and optimizing the treatment regimen, more patients can be treated with better regimens in a prolonged duration to achieve better efficacy and survival, especially in elderly and non-high-risk patients.
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http://dx.doi.org/10.3389/fphar.2020.561601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759685PMC
December 2020

Beyond Blocking: Engineering RNAi-Mediated Targeted Immune Checkpoint Nanoblocker Enables T-Cell-Independent Cancer Treatment.

ACS Nano 2020 Dec 8. Epub 2020 Dec 8.

Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China.

The emergence of immune checkpoint blockade to activate host T cells to attack tumor cells has revolutionized the cancer treatment landscape over the past decade. However, sustained response has only been achieved in a small proportion of patients. This can be attributed to physiological barriers, such as T-cell heterogeneity and immunosuppressive tumor microenvironments. To this can be added obstacles intrinsic to traditional antibody-driven blockade methods, including the inability to inhibit checkpoint translocation from cytoplasm, systemic immune toxicity, and "bite back" effect on T cells. Using non-small cell lung cancer (NSCLC) as the cancer model, here we report an unconventional, yet powerful, tumor-targeted checkpoint blocking strategy by RNAi nanoengineering for T-cell-independent cancer therapy. Unlike antibodies, such nanoblocker silences both membranous and cytoplasmic PD-L1 in cancer cells, thus eliminating the binding step. Moreover, it is demonstrated that silencing of PD-L1 by the nanoblocker can cause the direct programmed cell death of NSCLC H460 cells, without the need of T-cell intervention. results from xenograft tumor models further demonstrate that tumor-homing peptide modification enables the nanoblocker to accumulate in the tumor tissue, downregulate the PD-L1 expression, and inhibit the tumor growth more efficiently than the nontargeted group. These findings may offer an effective means toward overcoming barriers against traditional checkpoint blockade and provide different insights into the molecular mechanism(s) underlying immunotherapy.
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http://dx.doi.org/10.1021/acsnano.0c08022DOI Listing
December 2020

Three-Year Outcomes of Cionni-Modified Capsular Tension Ring Implantation in Children Under 8 Years Old With Ectopia Lentis.

Am J Ophthalmol 2020 Nov 28;224:74-83. Epub 2020 Nov 28.

Department of Ophthalmology, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, People's Republic of China; Key Laboratory of Myopia, Ministry of Health, Shanghai, People's Republic of China; Key Laboratory of Visual Impairment and Restoration, Shanghai, People's Republic of China; Visual Rehabilitation Professional Committee, Chinese Association of Rehabilitation Medicine, People's Republic of China. Electronic address:

Purpose: This study evaluated visual outcomes and complications at 3 years post-implantation of a Cionni-modified capsular tension ring (MCTR) with an intraocular lens (IOL) in ectopia lentis patients ≤8 years old.

Design: Prospective clinical cohort study.

Methods: Included were 101 eyes from 57 patients <8 years of age, who underwent surgery for nontraumatic ectopia lentis between November 2015 and December 2016. Exclusion criteria were planned IOL fixation in the ciliary sulcus, severe intraoperative complications, and incomplete follow-up. All eyes received in-the-bag implantation of a posterior IOL and Cionni-MCTR. Posterior capsulectomy and anterior vitrectomy were performed through the pars plana in 23 eyes of children <5 years of age. Patients were examined at 1 day, and 1, 6, and 12 months, and at 2 and 3 years postoperatively. Outcome; measurements included best-corrected visual acuity (BCVA), intraocular pressure (IOP), IOL centration, and posterior capsule opacification (PCO).

Results: In all eyes, BCVA improved significantly after surgery, especially during the first 12 months (P < .05). Three years post-operatively, 44 eyes had BCVA 0.9 or better. Prophylactic Nd:YAG laser capsulotomy was performed 3 months post-surgery in 24 eyes; 34 eyes underwent this; procedure 6 months post-surgery because of PCO. A second surgery was; warranted in 4 eyes because of severe IOL decentration and combined anterior capsule contraction. No severe postoperative complications, such as retinal detachment or endophthalmitis, occurred.

Conclusions: Implantation of in-the-bag IOL with Cionni MCTR is effective for visual rehabilitation in young children with ectopia lentis. A close follow-up of these patients is necessary to monitor IOL centration and stability.
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http://dx.doi.org/10.1016/j.ajo.2020.11.011DOI Listing
November 2020

Arthrobacter sulfonylureivorans sp. nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.

Arch Microbiol 2021 Apr 2;203(3):1039-1045. Epub 2020 Nov 2.

Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People's Republic of China.

A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil. The optimal temperature and pH for the growth of strain LAM7117 were 35 °C and 7.5, respectively. Strain LAM7117 could grow in the presence of NaCl with concentration up to 9% (w/v). Strain LAM7117 formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522 (97.7%). The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117 and A. crystallopoietes JCM 2522 were 21.4 and 77.4%, respectively. The genomic DNA G + C content was 65.9 mol%. The major cellular fatty acids were anteiso-C, iso-C and anteiso-C. The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid. The major polar lipids present in strain LAM7117 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid. The predominant menaquinones of strain LAM7117 were MK-8 and MK-9. Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117 should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp. nov. is proposed. The type strain is LAM7117 (= JCM 32824 = CGMCC 1.16681).
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http://dx.doi.org/10.1007/s00203-020-02097-2DOI Listing
April 2021

Π electron-stabilized polymeric micelles potentiate docetaxel therapy in advanced-stage gastrointestinal cancer.

Biomaterials 2021 Jan 5;266:120432. Epub 2020 Oct 5.

Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China. Electronic address:

Gastrointestinal (GI) cancers are among the most lethal malignancies. The treatment of advanced-stage GI cancer involves standard chemotherapeutic drugs, such as docetaxel, as well as targeted therapeutics and immunomodulatory agents, all of which are only moderately effective. We here show that Π electron-stabilized polymeric micelles based on PEG-b-p(HPMAm-Bz) can be loaded highly efficiently with docetaxel (loading capacity up to 23 wt%) and potentiate chemotherapy responses in multiple advanced-stage GI cancer mouse models. Complete cures and full tumor regression were achieved upon intravenously administering micellar docetaxel in subcutaneous gastric cancer cell line-derived xenografts (CDX), as well as in CDX models with intraperitoneal and lung metastases. Nanoformulated docetaxel also outperformed conventional docetaxel in a patient-derived xenograft (PDX) model, doubling the extent of tumor growth inhibition. Furthermore, micellar docetaxel modulated the tumor immune microenvironment in CDX and PDX tumors, increasing the ratio between M1-and M2-like macrophages, and toxicologically, it was found to be very well-tolerated. These findings demonstrate that Π electron-stabilized polymeric micelles loaded with docetaxel hold significant potential for the treatment of advanced-stage GI cancers.
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http://dx.doi.org/10.1016/j.biomaterials.2020.120432DOI Listing
January 2021

Actinoplanes solisilvae sp. nov., Isolated from Birch Forest Soil.

Curr Microbiol 2020 Nov 11;77(11):3799-3806. Epub 2020 Sep 11.

Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

A novel actinomycete, designated strain LAM7112, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The new isolate was found to be able to grow at 20-45 °C (optimum: 35 °C), pH 5.0-10.0 (optimum: 7.0) and in the presence of 0-10.0% (optimum: 3.0%) (w/v) NaCl. The isolate formed very scantily irregular sporangia containing motile spores on the substrate mycelium. Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolate was closely related to members of the family Micromonosporaceae, with highest similarites to Actinoplanes ferrugineus X-14695 (97.4%), Micromonospora zamorensis DSM 45600 (97.3%) and Micromonospora aurantiaca ATCC 27029 (97.3%). In the phylogenetic trees, strain LAM7112 formed a stable phylogenetic subclade within the genus Actinoplanes. The genomic DNA G + C content was 70.0 mol%. The major fatty acids (> 10%) were determined to be iso-C, anteiso-C and anteiso-C. The predominant menaquinones were identified as MK-9 (H), MK-9 (H) and MK-9 (H). The major polar lipids were found to be diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The diagnostic amino acid of the cell wall peptidoglycan was determined to be meso-diaminopimelic acid. The diagnostic sugars in cell hydrolysates were determined to be glucose and ribose. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM7112 (= CGMCC 4.7580 = JCM 32512) is proposed to represent the type strain of a novel species of the genus Actinoplanes, for which the name Actinoplanes solisilvae is proposed.
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http://dx.doi.org/10.1007/s00284-020-02192-zDOI Listing
November 2020

ABCA1 plays an anti-inflammatory role by affecting TLR4 at the feto-maternal interface.

Life Sci 2020 Oct 5;259:118390. Epub 2020 Sep 5.

Department of Gynecology and Obstetrics, Beijing Friendship Hospital, Capital Medical University, 100050 Beijing, China. Electronic address:

Aims: This study aimed to evaluate the function and pathway of ATP-binding cassette transporter member A1 (ABCA1)-induced anti-inflammatory response in cells at the feto-maternal interface.

Main Methods: The primary amniotic mesenchymal cells (AMCs), chorion cells and decidual cells were isolated from placental membranes of women with uncomplicated pregnancies at full-term (not in labor) using enzymatic digestion. Flow cytometry was used to measure the purity of isolated cells. Immunofluorescence assay was performed to detect the location of ABCA1 and toll-like receptor 4 (TLR4). Reverse transcription PCR and western blotting analyses were used to examine ABCA1, TLR4 and inflammatory factor expression in primary cells. ELISA was used to detect cytokine secretions from the primary cells.

Key Findings: ABCA1 and TLR4 were mainly located in the cell nucleus and cytoplasm of feto-maternal interface cells. ABCA1 expression remained the highest in chorion cells, medium in decidual cells, and weakest in AMCs. Upregulated expression of ABCA1 decreased expression of TLR4 and the levels of pro-inflammatory factors, but increased cytoprotective factors in all cell types. In contrast, downregulated expression of ABCA1 increased the expression of TLR4 and pro-inflammatory factors, but decreased the levels of cytoprotective factors. Downregulated ABCA1 expression followed by decreased TLR4 expression using a small interference RNA (siRNA) induced reduction of interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in all cell types.

Significance: ABCA1 at feto-maternal interface acts as an anti-inflammatory role by reducing the expression of TLR4 in uncomplicated pregnancies. ABCA1 might be a potential therapeutic target for preventing gestational diseases.
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http://dx.doi.org/10.1016/j.lfs.2020.118390DOI Listing
October 2020

Mycophenolate mofetil in the treatment of Chinese patients with lupus nephritis: A PRISMA-compliant meta-analysis.

Medicine (Baltimore) 2020 Aug;99(33):e21121

Shanghai Roche Pharmaceuticals Ltd., Shanghai, PR China.

Backgroud: Mycophenolate mofetil (MMF) has been recommended for the treatment of lupus nephritis (LN). Although inter-racial differences exist regarding the appropriate dose and efficacy of MMF in patients with LN, no definitive meta-analysis has yet been conducted in Chinese patients. This analysis investigated the efficacy and safety of MMF in Chinese patients with proliferative LN.

Methods: A systematic literature search was conducted to select randomized controlled trials that reported at least one of the following: complete remission (CR), partial remission, total remission (TR; defined as complete remission + partial remission), relapse rate, serum creatinine, creatinine clearance, end-stage renal disease, death, infections, amenorrhea, leukopenia, alopecia, gastrointestinal symptoms, or liver damage.

Results: Eighteen trials (927 patients) were included; 14 (750 patients) reported CR, partial remission, and TR. Two trials (58 patients) reported relapse rates during maintenance treatment. MMF induction significantly improved CR and TR vs cyclophosphamide (relative risk 1.34, 95% confidence interval: 1.13-1.58; P < .001; relative risk 1.16, 95% confidence interval: 1.02-1.33; P = .03), and was associated with significantly lower risks of infection (P < .001), amenorrhea (P < .001), leukopenia, and alopecia. No significant difference in relapse rate was evident between the MMF and azathioprine groups (P = .66).

Conclusion: According to this meta-analysis of 18 trials, MMF is significantly more effective than cyclophosphamide induction, and is associated with reduced incidences of infections, amenorrhea, leukopenia, and alopecia in Chinese patients with proliferative LN.
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http://dx.doi.org/10.1097/MD.0000000000021121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7437744PMC
August 2020

Impact of intervention methods on COVID-19 transmission in Shenzhen.

Build Environ 2020 Aug 2;180:107106. Epub 2020 Jul 2.

Department of Mechanical Engineering, The University of Hong Kong, Hong Kong SAR, China.

By March 31, 2020, COVID-19 had spread to more than 200 countries. Over 750,000 confirmed cases were reported, leading to more than 36,000 deaths. In this study, we analysed the efficiency of various intervention strategies to prevent infection by the virus, SARS-CoV-2, using an agent-based SEIIR model, in the fully urbanised city of Shenzhen, Guangdong Province, China. Shortening the duration from symptom onset to hospital admission, quarantining recent arrivals from Hubei Province, and letting symptomatic individuals stay at home were found to be the three most important interventions to reduce the risk of infection in Shenzhen. The ideal time window for a mandatory quarantine of arrivals from Hubei Province was between 10 January and January 17, 2020, while the ideal time window for local intervention strategies was between 15 and 22 January. The risk of infection could have been reduced by 50% if all symptomatic individuals had immediately gone to hospital for isolation, and by 35% if a 14-day quarantine for arrivals from Hubei Province had been introduced one week earlier. Intervention strategies implemented in Shenzhen were effective, and the spread of infection would be controlled even if the initial basic reproduction number had doubled. Our results may be useful for other cities when choosing their intervention strategies to prevent outbreaks of COVID-19.
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http://dx.doi.org/10.1016/j.buildenv.2020.107106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331564PMC
August 2020

Associations of long-term exposure to ambient air pollution with cardiac conduction abnormalities in Chinese adults: The CHCN-BTH cohort study.

Environ Int 2020 10 29;143:105981. Epub 2020 Jul 29.

Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University, and Beijing Municipal Key Laboratory of Clinical Epidemiology, Beijing, China. Electronic address:

Background: Evidence regarding the effects of long-term and high-level ambient air pollution exposure on cardiac conduction systems remains sparse.

Objectives: To investigate the associations of long-term exposure to air pollution and cardiac conduction abnormalities in Chinese adults and explore the susceptibility characteristics.

Methods: In 2017, a total of 27,047 participants aged 18-80 years were recruited from the baseline survey of the Cohort Study on Chronic Disease of Communities Natural Population in Beijing, Tianjin and Hebei (CHCN-BTH). The three year (2014-2016) average pollutant concentrations were assessed by a spatial statistical model for PM and air monitoring stations for PM, SO, NO, O and CO. Residential proximity to a roadway was calculated by neighborhood analysis. Associations were estimated by two-level generalized linear mixed models. Stratified analyses related to demographic characteristics, health behaviors, and cardiometabolic risk factors were performed. Two-pollutant models were used to evaluate the possible role of single pollutants.

Results: We detected significant associations of long-term air pollutant exposure with increased heart rate (HR), QRS and QTc, such that an interquartile range increase in PM was associated with 3.63% (95% CI: 3.07%, 4.19%), 1.21% (95% CI: 0.83%, 1.60%), and 0.13% (95% CI: 0.07%, 0.18%) changes in HR, QRS and QTc, respectively. Compared to the other pollutants, the estimates of PM remained the most stable across all two-pollutant models. Similarly, significant associations were observed between living closer to a major roadway and higher HR, QRS and QTc. Stratified analyses showed generally greater association estimates in older people, males, smokers, alcohol drinkers, and those with obesity, hypertension and diabetes.

Conclusions: Long-term exposure to ambient air pollution was associated with cardiac conduction abnormalities in Chinese adults, especially in older people, males, smokers, alcohol drinkers, and those with cardiometabolic risk factors. PM may be the most stable pollutant to reflect the associations.
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http://dx.doi.org/10.1016/j.envint.2020.105981DOI Listing
October 2020

Identification of tRNA-derived fragments and their potential roles in diabetic cataract rats.

Epigenomics 2020 08 23;12(16):1405-1418. Epub 2020 Jul 23.

Department of Ophthalmology & the Eye Institute, Eye & Ear, Nose, & Throat Hospital, Fudan University, Shanghai 200031, PR China.

To illustrate the expression profile of transfer RNA-derived fragments and reveal their putative role in the pathogenesis of diabetic cataract (DC) rats. Small RNA sequencing was conducted in the lens epithelium of rats lens. The data were validated by quantitative real-time PCR, and bioinformatic analysis was performed to explore the roles of the fragments in DC pathogenesis. A total of 213 differentially expressed tRNA-related fragments were identified, in which 111 were upregulated and 102 were downregulated in DC rats. Bioinformatics analysis revealed that several associated pathways might participate in the development of DC rats. tRNA-derived fragments may be involved in the pathogenesis of DC rats.
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http://dx.doi.org/10.2217/epi-2020-0193DOI Listing
August 2020

Patient Advocates for Low-Income Adults with Moderate to Severe Asthma: A Randomized Clinical Trial.

J Allergy Clin Immunol Pract 2020 Nov - Dec;8(10):3466-3473.e11. Epub 2020 Jul 14.

Department of Biostatistics, Epidemiology and Informatics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pa.

Background: Few interventions have targeted low-income adults with moderate to severe asthma despite their high mortality.

Objective: To assess whether a patient advocate (PA) intervention improves asthma outcomes over usual care (UC).

Methods: This 2-armed randomized clinical trial recruited adults with moderate to severe asthma from primary care and asthma-specialty practices serving low-income neighborhoods. Patients were randomized to 6 months of a PA intervention or UC. PAs were recent college graduates anticipating health care careers, who coached, modeled, and assisted participants with preparations for asthma-related medical visits, attended visits, and confirmed participants' understanding of provider recommendations. Participants were followed for at least a year for patient-centered asthma outcomes: asthma control (primary outcome), quality of life, prednisone requirements, emergency department visits, and hospitalizations.

Results: There were 312 participants. Their mean age was 51 years (range, 19-93 years), 69% were women, 66% African American, 8% Hispanic/Latino, 62% reported hospitalization for asthma in the year before randomization, 21% had diabetes, and 61% had a body mass index of 30 or more. Asthma control improved over 12 months, more in the intervention group (-0.45 [95% CI, -0.67 to -0.21]) than in the UC group (-0.26 [95% CI, -0.53 to -0.01]), and was sustained at 24 months but with no statistical difference between groups. The 6-month rate of emergency department visits decreased in the intervention (-0.90 [95% CI, -1.56 to -0.42]) and UC (-0.42 [95% CI, -0.72 to -0.06]) groups over 12 months. The cost of the PA program was $1521 per patient. Only 64% of those assigned had a PA visit.

Conclusions: A PA may be a promising intervention to improve and sustain outcomes in this high-risk population if expanded to address factors that make keeping appointments difficult.
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http://dx.doi.org/10.1016/j.jaip.2020.06.058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924969PMC
July 2020

Three cases of lung cancer in pregnancy and literature review.

Ann Palliat Med 2020 Jul 9;9(4):1928-1936. Epub 2020 Jul 9.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: The incidence of tumors during pregnancy is 1ntscomprising 0.07-0.1% of all malignant tumors. Lung cancer during pregnancy is rare, but it is the second leading cause of cancer-attributed mortality in women of childbearing age. This article reports 3 cases of lung cancer in pregnant women and reviews the relevant literature, to explore the diagnosis, treatment, and prognosis of women with lung cancer during pregnancy.

Methods: The clinical and pathological data of 3 pregnant women with lung cancer who were treated at our hospital were retrospectively analyzed, and the relevant literature was reviewed.

Results: The three patients with lung cancer during pregnancy were all under 40 years old, and the gestational weeks of diagnosis were 20, 22 and 36 weeks, respectively. Two of the women were clinical stage IV and 1 was clinical stage III, all of which constitute advanced lung cancer. Pathological types are adenocarcinoma, small cell neuroendocrine carcinoma, and adenocarcinoma with neuroendocrine carcinoma respectively. Two patients were diagnosed during the second trimester and had mid-term induction of labor. After lung cancer surgery in other hospitals, they were died at 4 and 7 months after diagnosis. The remaining patient, who was diagnosed at 36 gestational week underwent cesarean section at 37 weeks. The patient received chemoradiotherapy, and was still alive at 19 months postpartum follow-up.

Conclusions: Lung cancer during pregnancy is rare in clinic. For those who repeatedly experience respiratory symptoms during pregnancy, imaging examinations should be performed promptly, and biopsies should be taken if necessary to obtain early diagnosis. Pregnancy with lung cancer carries high risk and has a poor overall prognosis. Platinum-based combination chemotherapy is safe in the second and third trimesters.
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http://dx.doi.org/10.21037/apm-20-999DOI Listing
July 2020

Clinical Characteristics of Intraocular Lens Dislocation in Chinese Han Populations.

J Ophthalmol 2020 26;2020:8053941. Epub 2020 Apr 26.

Department of Ophthalmology and the Eye Institute, Eye and Ear, Nose, and Throat Hospital, Fudan University, Shanghai, China.

Purpose: To investigate the clinical characteristics of patients with intraocular lens (IOL) dislocation after IOL implantation in Chinese Han populations.

Methods: The medical records of patients with IOL dislocation were retrospectively reviewed from January 2007 to December 2017, and a total of 312 patients (male: 231, female: 97) (328 eyes) were included in this study. The axial length (AL), IOL power, and the time interval between cataract surgery and IOL dislocation as well as the ocular conditions associated with IOL dislocation were recorded. The IOL dislocation was classified and graded based on its relationship with the capsule and the position of the dislocated IOL.

Results: The mean time between original cataract surgery and IOL dislocation was 5.63 ± 5.13 years; IOL dislocation occurred in up to 56.1% (184 eyes) of the eyes within 5 years. Trauma was found in 136 eyes (41.5%); pars plana vitrectomies were performed in 61 eyes (18.6%), and high myopia was detected in 108 eyes (32.9%). A total of 243 eyes (74.1%) had out-of-the-bag IOL dislocations, while 85 eyes (25.9%) had in-the-bag IOL dislocations. There was a statistically significant difference in the constituent ratio of trauma between in-the-bag dislocation and out-of-the-bag dislocation (Pearson's chi = 33.3992, < 0.001); ocular blunt traumas were significantly higher in in-the-bag dislocations, while open-globe injuries were significantly higher in out-of-the-bag dislocations. A statistically significant difference was found for the ratio of patients with AL longer than 30 mm between in-the-bag dislocation and out-of-the-bag dislocation (Pearson's chi = 9.7355, < 0.002).

Conclusions: In Chinese Han populations, the most common IOL dislocation is out-of-the-bag dislocation; the most common risk factors were trauma, long axial length, and eyes undergoing pars plana vitrectomy; a minimum follow-up of 5 years is suggested for IOL dislocation-predisposed eyes undergoing cataract surgery.
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http://dx.doi.org/10.1155/2020/8053941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201720PMC
April 2020

Antioxidant properties and neuroprotective effects of Esc-1GN through the regulation of MAPK and AKT signaling.

Life Sci 2020 Aug 6;254:117753. Epub 2020 May 6.

Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. Electronic address:

Aims: This study aimed to explore the antioxidant properties and neuroprotective effects of Esc-1GN.

Main Methods: FRAP assay and ABTS, DPPH, and NO radicals radical scavenging assays were performed to investigated the Antioxidant activities of Esc-1GN in vitro. Hydrogen peroxide (HO)-induced cell damage model was used to determine the neuroprotective effects of Esc-1GN. Carrageenan-injected inflamed paw model was performed to analysis the antioxidant and anti-inflammatory properties of Esc-1GN in vivo.

Key Findings: Esc-1GN scavenged the ABTS, DPPH, and NO radicals and reduced Fe in a concentration-dependent manner. Moreover, Esc-1GN exhibited neuroprotective activity by decreasing malondialdehyde and reactive oxygen species accumulation, restoring endogenous antioxidant enzyme activity, and inhibiting HO-induced cell cycle arrest and apoptosis in PC12 cells. Esc-1GN significantly reversed the dysregulation of MAPK, AKT and NF-κB signaling caused by HO. In vivo, Esc-1GN decreased MDA, COX-2, NO, TNF-α, IL-6, and Il-1β levels and increased SOD, CAT activity and GSH level in carrageenan-injected inflamed paw tissues.

Significance: These findings suggest that Esc-1GN might serve as a potential antioxidant agent with therapeutic potential in human neurodegenerative diseases.
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http://dx.doi.org/10.1016/j.lfs.2020.117753DOI Listing
August 2020

sp. nov., isolated from birch forest soil.

Int J Syst Evol Microbiol 2020 Apr 15;70(4):2690-2695. Epub 2020 Apr 15.

Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.

A Gram-stain-positive, motile, rod-shaped bacterium, designated strain LAM7113, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, PR China. Strain LAM7113 grew optimally at pH 8.0, 30 °C and in the presence of 1.0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LAM7113 was closely related to members of the genus , with the highest similarity to Back-11 (96.2 %). The genomic DNA G+C content was 43.4 mol%. The values of average nucleotide identity and DNA-DNA hybridization were 66.1 and 27.0 %, respectively, by comparing the draft genome sequences of strain LAM7113 and Back-11. Anteiso-C and iso-C were identified as the major cellular fatty acids. Menaquinone-7 was detected as the predominant respiratory quinone. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified aminophospholipids, three unidentified glycolipids, one unidentified phospholipid and two unknown polar lipids. Based on its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM7113 is proposed to represent a novel species of the genus with the name sp. nov. The type strain is LAM7113 (=CGMCC 1.16619=JCM 32513).
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http://dx.doi.org/10.1099/ijsem.0.004093DOI Listing
April 2020

Streptomyces soli sp. nov., isolated from birch forest soil.

Arch Microbiol 2020 Sep 13;202(7):1687-1692. Epub 2020 Apr 13.

Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, China.

A Gram-staining positive, aerobic, non-motile, rod-shaped bacterium, designated strain LAM7114, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The optimal temperature and pH for the growth of strain LAM7114 were 30 °C and 7.0, respectively. Strain LAM7114 could grow in the presence of NaCl up to 10% (w/v). Comparative analysis of the 16S rRNA gene sequences revealed that LAM7114 was closely related to the members of the family genus Streptomyces, with the highest similarity to Streptomyces urticae NEAU-PCY-1 (98.3%) and Streptomyces fildesensis GW25-5 (98.2%). The genomic G + C content was 70.0 mol%. The DNA-DNA hybridization values between strain LAM7114 and S. urticae CCTCC AA 2017015, S. fildesensis CGMCC 4.5735 were 32.5 ± 1.8% and 27.5 ± 2.6%, respectively. The cell wall contained LL-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The whole-cell hydrolysates included glucose and mannose. The major fatty acids were anteiso-C, iso-C and iso-C. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, three unidentified aminophospholipids, three unidentified phospholipids, and an unidentified aminolipid. Based on the phenotypic characteristics and genotypic analyses, we propose that strain LAM7114 represents a novel species in the genus Streptomyces, for which the name Streptomyces soli sp. nov. is proposed. The type strain is LAM7114 (= CGMCC 4.7581 = JCM 32822).
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http://dx.doi.org/10.1007/s00203-020-01878-zDOI Listing
September 2020

Changes to cardiovascular risk factors over 7 years: a prospective cohort study of in situ urbanised residents in the Chaoyang District of Beijing.

BMJ Open 2020 03 16;10(3):e033548. Epub 2020 Mar 16.

Office for Epidemiology, Chinese Center for Disease Control and Prevention, Beijing, China

Objectives: To examine changes in cardiovascular risk factors of in situ urbanised residents between 2010 and 2017.

Design: Population-based cohort study.

Setting: The Chaoyang District of Beijing, China.

Participants: A total of 942 in situ urbanised rural residents aged 35-64 who participated in the cardiovascular disease (CVD) risk factors study in China between 2010 and 2017.

Main Outcome Measures: Lifestyles (smoking, drinking and effective exercise) and medical history (diabetes, hypertension, dyslipidaemia, overweight and obesity) were self-reported. New cases of diabetes, hypertension, dyslipidaemia, overweight and obesity were confirmed by physical examination or blood biochemical tests. Multiple linear regression and log-binomial models analyses adjusted for sociodemographic confounders were conducted to evaluate any changes of clinical indexes and to estimate prevalence rate ratios (PRRs), respectively.

Results: During the study period of 2010-2017, diastolic blood pressure elevated by 3.55 mm Hg, central blood pressure increased by 4.39 mm Hg, total cholesterol decreased by 0.29 mmol/L and hypertension increased significantly (PRR=1.25, p<0.05) after adjusting for demographic, lifestyle and family history factors. Effective exercise rate (PRR=1.57), prevalence of diabetes (PRR=1.36) and dyslipidaemia (PRR=1.19) all increased from 2010 to 2017. However, these changes were not significant after adjusting for confounders (p0.05). Prevalence of smoking, drinking, hypertension, overweight and obesity was significantly higher in males than females in both 2010 and 2017. In 2017, the 10-year risk of atherosclerotic CVD increased in 29.8% of participants and decreased in 6.1% of individuals.

Conclusions: CVD risk factors augmented remarkably for in situ urbanised rural residents aged 35-64 in the Chaoyang District of Beijing, especially those indicators related to blood pressure. Awareness of the direction and magnitude of these risk factor changes may be beneficial in informing targeted strategies for preventing CVDs of in situ urbanised populations.
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http://dx.doi.org/10.1136/bmjopen-2019-033548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076243PMC
March 2020

Central rather than brachial pressures are stronger predictors of cardiovascular outcomes: A longitudinal prospective study in a Chinese population.

J Clin Hypertens (Greenwich) 2020 04 9;22(4):623-630. Epub 2020 Mar 9.

Division of Prevention and Community Health, National Center for Cardiovascular Disease, State Key Laboratory of Cardiovascular Disease, National Clinical Research Center of Cardiovascular Disease, Fuwai Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

The purpose of this study was to assess the association of blood pressure (BP) measurements with the risk of cardiovascular disease (CVD) and examine whether central systolic BP (CSBP) predicts CVD better than brachial BP measurements (SBP and pulse pressure [PP]). Based on a cross-sectional study conducted in 2009-2010 with follow-up in 2016-2017 among 35- to 64-year-old subjects in China, we evaluated the performance of non-invasively predicted CSBP over brachial BP measurements on the first CVD events. Each BP measurement, individually and jointly with another BP measurement, was entered into the multivariate Cox proportional-hazards models, to examine the predictability of central and brachial BP measurements. Mean age of participants (n = 8710) was 50.1 years at baseline. After a median follow-up of 6.36 years, 187 CVD events occurred. CSBP was a stronger predictor for CVD than brachial BP measurements (CSBP, 1-standard deviation increment HR = 1.49, 95%CI: 1.31-1.70). With CSBP and SBP entering into models jointly, the HR for CSBP and SBP was 1.28 (1.04-1.58) and 1.22 (0.98-1.50), respectively. With CSBP and PP entering into models jointly, the HR for CSBP and PP was 1.51 (1.28-1.78) and 0.98 (0.83-1.15), respectively. For subgroup analysis, the association of CSBP with CVD was stronger than brachial BP measurements in women, those with hypertension and obesity. In the middle-aged Chinese population, noninvasively estimated CSBP may offer advantages over brachial BP measurements to predict CVD events, especially for participants with higher risk. These findings suggest prospective assessment of CSBP as a prevention and treatment target in further trials.
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http://dx.doi.org/10.1111/jch.13838DOI Listing
April 2020

Genome-wide analyses reveals a glucosyltransferase involved in rutin and emodin glucoside biosynthesis in tartary buckwheat.

Food Chem 2020 Jul 25;318:126478. Epub 2020 Feb 25.

Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address:

With people's increasing needs for health concern, rutin and emodin in tartary buckwheat have attracted much attention for their antioxidant, anti-diabetic and reducing weight function. However, the biosynthesis of rutin and emodin in tartary buckwheat is still unclear; especially their later glycosylation contributing to make them more stable and soluble is uncovered. Based on tartary buckwheat' genome, the gene structures of 106 UGTs were analyzed; 21 candidate FtUGTs were selected to enzymatic test by comparing their transcript patterns. Among them, FtUGT73BE5 and other 4 FtUGTs were identified to glucosylate flavonol or emodin in vitro; especially rFtUGT73BE5 could catalyze the glucosylation of all tested flavonoids and emodin. Furthermore, the identical in vivo functions of FtUGT73BE5 were demonstrated in tartary buckwheat hairy roots. The transcript profile of FtUGT73BE5 was consistent with the accumulation trend of rutin in plant; this gene may relate to anti-adversity for its transcripts were up-regulated by MeJA, and repressed by ABA.
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http://dx.doi.org/10.1016/j.foodchem.2020.126478DOI Listing
July 2020