Publications by authors named "Xiaoyan Fan"

50 Publications

HIV diagnosis period influences ART initiation: findings from a prospective cohort study in China.

AIDS Res Ther 2021 09 9;18(1):59. Epub 2021 Sep 9.

School of Public Health, Sun Yat-sen University, No. 74 Zhongshan Road 2, Guangzhou, 510080, Guangdong, China.

Background: We estimated the predictive effects of ART-related perceptions on the actual ART uptake behavior among ART naïve PLWH stratified by different time of HIV diagnosis under the new strategy.

Methods: A prospective cohort study was conducted among ART naïve PLWH in Guangzhou, China from June 2016 to June 2017. Cox regression model was used to evaluate the predictive effects of ART-related perceptions on ART initiation among PLWH stratified by different timepoint of HIV diagnosis (i.e., before or after the update of the new treatment policy).

Results: Among 411 participants, 150 and 261 were diagnosed before (pre-scaleup group) and after (post-scaleup group) the implementation of the new strategy, respectively. The ART initiation rate in the post-scaleup group (88.9%) was higher than that in the pre-scaleup group (73.3%) (p < 0.001). A significant difference of mean score was detected in each HBM construct between pre- and post-scaleup groups (p < 0.05). After adjusting for significant background variables, among all participants, only the self-efficacy [adjusted HR (HRa) = 1.23, 95% CI 1.06 to 1.43, p = 0.006], has a predictive effect on ART initiation; in pre-scaleup group, all constructs of HBM-related ART perceptions were predictors of ART initiation (HRa = 0.71 to 1.83, p < 0.05), while in post-scaleup group, no significant difference was found in each construct (p > 0.05).

Conclusions: The ART initiation rate was high particularly among participants who diagnosed after the new treatment strategy. The important role of the time of HIV diagnosis on ART initiation identified in this study suggested that future implementation interventions may consider to modify the ART-related perceptions for HIV patients who diagnosed before the implementation of the new ART strategy, while expand the accessibility of ART service for those who diagnosed after the implementation of the new strategy.
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http://dx.doi.org/10.1186/s12981-021-00379-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428057PMC
September 2021

B-Myb accelerates colorectal cancer progression through reciprocal feed-forward transactivation of E2F2.

Oncogene 2021 Jul 27. Epub 2021 Jul 27.

Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China.

B-Myb is an important transcription factor that plays a critical role in gene expression regulation and tumorigenesis. However, its functional implication in colorectal cancer remains elusive. In this study, we found that B-Myb was significantly upregulated at both mRNA and protein levels in colorectal cancer samples compared to non-tumor counterparts. B-Myb overexpression accelerated cell proliferation, cell cycle progression and cell motility in colorectal cancer cells, and promoted tumor growth in orthotopic nude mouse models in vivo. In contrast, B-Myb depletion inhibited these malignant phenotypes. Mechanistic investigations revealed that E2F2 was a novel transcriptional target of B-Myb and is essential to B-Myb-induced malignant phenotypes. Notably, B-Myb and E2F2 exhibited positive expression correlation, and interacted with each other in colorectal cancer cells. In addition to their autoregulatory mechanisms, B-Myb and E2F2 can also directly transactivate each other, thus constituting consolidated reciprocal feed-forward transactivation loops. Moreover, both B-Myb and E2F2 are required for the activation of ERK and AKT signaling pathways in colorectal cancer cells. Taken together, our data clarified a critical role for B-Myb in colorectal cancer and unraveled an exquisite mutual collaboration and reciprocal cross regulation between B-Myb and E2F2 that contribute to the malignant progression of human colorectal cancer.
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http://dx.doi.org/10.1038/s41388-021-01961-9DOI Listing
July 2021

Keystone taxa-mediated bacteriome response shapes the resilience of the paddy ecosystem to fungicide triadimefon contamination.

J Hazard Mater 2021 09 9;417:126061. Epub 2021 May 9.

Institute of Pesticide and Environmental Toxicology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China; Global Education Program for AgriScience Frontiers, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address:

The increasing input of fungicides has emerged as a global concern for agroecosystem stability and sustainability. Agroecosystem resilience has been linked to microbiome response, however, is not well understood. Focusing on a widespread triazole-class fungicide triadimefon in the paddy ecosystem, we characterized that the soils and sediments were dominant triadimefon reservoirs with the peak level at 195 μg kg and 31.3 μg kg, respectively, but essential for the resilience of paddy ecosystem to triadimefon. In paddy simulation models, the half-life of triadimefon in soil-sediment was 8.4-28.9 days, while it was prolonged to 86.6-115.5 days after elimination of resident microbial community. Phospholipid fatty acid profiling and high-throughput sequencing showed that the distinctive bacterial community responses contributed to variable degradation of triadimefon in paddy soils and sediments. Sphingomonas and Xanthomonas were identified as positive responders of the keystone taxa in the responsive bacteriome, whereas Enterobacter were negative responders that declined over time. Synthetic assemblages combined with quantitative polymerase chain reaction further validated that Sphingomonas and Xanthomonas were involved in sustaining soil-sediment resilience to triadimefon contamination. Collectively, our results revealed that the shaping of soil and sediment bacteriomes was responsible for the resilience of the paddy agroecosystem to fungicide contamination.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126061DOI Listing
September 2021

Effects of - and spp.-Based Potential Probiotics on the Growth Performance, Intestinal Morphology, Immune Responses, and Caecal Microbiota in Broilers.

Antibiotics (Basel) 2021 May 24;10(6). Epub 2021 May 24.

Key Laboratory of Applied Technology on Green-Eco-Health Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, Hangzhou 311300, China.

We aimed to investigate the effects of -, -, and -based potential probiotics on the growth performance, intestinal morphology, immune responses, and caecal short chain fatty acids (SCFAs) and microbial structure in broiler chickens. Three treatment groups containing a total of 1200 one-day-old AA broilers were included: birds fed with a basal diet only (Con), birds fed with added 10 probiotics cfu/kg (ProL), and birds fed with added 10 probiotics cfu/kg (ProH). The dietary probiotics significantly improved the final and average body weights and serum immunoglobulins A, M, and Y. The probiotics also enhanced the ileal morphology and improved the caecal acetate, butyrate, and propionate contents. Furthermore, 16S rRNA sequencing revealed that dietary compound probiotics modulated the caecal microflora composition as follows: (1) all birds shared 2794 observed taxonomic units; (2) treatment groups were well separated in the PCA and PCoA analysis; (3) the relative abundance of , _UCG-014, , , [_coprostanoligenes_group], []_torques_group, and significantly varied between treatments. The compound probiotics improved the growth performance, serum immune responses, the ratio of ileal villus height to crypt depth, and major caecal SCFAs in broiler chickens. The dietary -, -, and -based probiotics improved overall broiler health and would benefit the poultry industry.
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http://dx.doi.org/10.3390/antibiotics10060624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225201PMC
May 2021

TACC3 is a prognostic biomarker for kidney renal clear cell carcinoma and correlates with immune cell infiltration and T cell exhaustion.

Aging (Albany NY) 2021 03 10;13(6):8541-8562. Epub 2021 Mar 10.

Department of Immune-Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, P.R. China.

Growing evidence has demonstrated that transforming acidic coiled-coil protein 3 (TACC3), a member of the TACC family, may be involved in regulating cell mitosis, transcription, and tumorigenesis. However, the role of TACC3 in kidney renal clear cell carcinoma (KIRC) remains unknown. In this study, multiple databases were used to determine the pattern of TACC3 in KIRC. We found that high TACC3 expression was associated with poor overall survival (OS) in stage I, II, IV and grade 3 KIRC patients. Univariate and multivariate Cox regression analyses showed that TACC3 was an independent risk factor for OS among KIRC patients. Moreover, TACC3 expression correlated with immune cell infiltration levels of B cells, T cells (CD8+, CD4+, follicular helper, regulatory and gamma delta), total and resting natural killer cells, total and activated dendritic cells, and resting mast cells. Furthermore, T cell exhaustion markers, such as PD1, CTLA4, LAG3 and TIM-3 were highly expressed in TACC3 overexpressing tissues. In addition, GSEA analysis revealed that the role of TACC3 in KIRC may be closely linked to immune-associated pathways. Therefore, our study reveals that TACC3 is a prognostic biomarker for OS among KIRC patients and may be associated with immune cell infiltration and T cell exhaustion.
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http://dx.doi.org/10.18632/aging.202668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034911PMC
March 2021

Nickel-vanadium layered double hydroxide nanosheets as the saturable absorber for a passively Q-switched 2  µm solid-state laser.

Appl Opt 2021 Mar;60(7):1851-1855

Nickel-vanadium (NiV)-layered double hydroxide (LDH) was fabricated into a novel saturable absorber (SA) by the liquid phase exfoliation method and utilized as the laser modulator for the first time, to our best knowledge. We investigated a passive -switched Tm:YAG ceramic laser at 2 µm with the NiV-LDH SA. Under an absorbed pump power of 7.2 W, the shortest pulse width of 398 ns was obtained with an average output power of 263 mW and a pulse repetition frequency of 101.8 kHz, corresponding to a single pulse energy at 2.30 µJ. The results indicate that the NiV-LDH SA has great research potential in the field of laser modulation.
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http://dx.doi.org/10.1364/AO.413803DOI Listing
March 2021

The differentiation of colorectal cancer is closely relevant to m6A modification.

Biochem Biophys Res Commun 2021 03 8;546:65-73. Epub 2021 Feb 8.

State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Fourth Military Medical University, 710032, Xi'an, China. Electronic address:

The occurrence and development of tumors cannot be separated from the influence of differentiation at different stages and levels. Our study found that E-cadherin was significantly increased in cell model induced by sodium butyrate and cell density, while METTL3, METTL16 and WTAP were decreased during the differentiation of cells. In the clinicopathological tissues, E-cadherin was low expressed in poorly differentiated tumor tissues and above three regulators were highly expressed in poorly differentiated tissues. At the levels of clinicopathological differentiation, tissue differentiation and cell differentiation, the result indicated that the poor prognosis of colorectal cancer (CRC) may be closely related to high expression of total m6A level and high expression of METTL3, METTL16 and WTAP.
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http://dx.doi.org/10.1016/j.bbrc.2021.02.001DOI Listing
March 2021

Association between B- proto-oncogene and the development of malignant tumors.

Oncol Lett 2021 Feb 4;21(2):166. Epub 2021 Jan 4.

Department of Basic Medical Sciences, Taizhou University, Taizhou, Zhejiang 318000, P.R. China.

B-Myb is a critical transcription factor in regulating cell cycle. Dysregulated expression of B-Myb promotes tumor formation and development. B- is a proto-oncogene ubiquitously expressed in proliferating cells, which maintains normal cell cycle progression. It participates in cell apoptosis, tumorigenesis and aging. In addition, B- is overexpressed in several malignant tumors, including breast cancer, lung cancer and hepatocellular carcinoma, and is associated with tumor development. B- expression is also associated with the prognosis of patients with malignant tumors. Both microRNAs and E2F family of transcription factors (E2Fs) contribute to the function of B-Myb. The present review highlights the association between B- and malignant tumors, and offers a theoretical reference for the diagnosis and treatment of malignant tumors.
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http://dx.doi.org/10.3892/ol.2021.12427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798104PMC
February 2021

N-myc downstream-regulated gene 2 promotes the protein stability of estrogen receptor beta via inhibition of ubiquitin-protein ligase E3A to suppress colorectal cancer.

J Gastrointest Oncol 2020 Dec;11(6):1200-1213

State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.

Background: N-myc downstream-regulated gene 2 (NDRG2) and estrogen receptor beta (ERβ) both play key roles in cellular differentiation in colorectal cancer (CRC). Previous studies have demonstrated that ERβ co-locates with and directly transactivates NDRG2. However, the effect of NDRG2 on ERβ and its underlying mechanism remain largely unknown. Our aim of the study is to explore the effect of NDRG2 on ERβ and their contributions to progression of CRC.

Methods: The Cancer Genome Atlas (TCGA) database was first utilized to validate the clinical significance of ERβ and NDRG2 in CRC. MTT and scratch migration assays were carried out to verify the role of ERβ and NDRG2 in CRC cells. Western blotting and polymerase chain reaction were performed to analyze the effect of NDRG2 on ERβ, and an immunoprecipitation assay was conducted to explore the protein-protein interaction.

Results: ERβ and NDRG2 were both found to be significantly down-regulated in tumor tissues from the TCGA-CRC database. NDRG2 was also observed to enhance the protein stability of ERβ while could not change messenger RNA (mRNA) level of ESR2 (encoding ERβ). A positive relationship was found to exist between the two proteins in CRC cells, with NDRG2 prolonging the half-life of ERβ and improving its nuclear translocation. Through detecting expression of ERβ downstream genes (such as TP53 and JNK) and performing related function experiment, we demonstrated that NDRG2 could promote transcriptional activation of ERβ target genes and enhance the function of tumor suppressors when the ERβ agonist diarylpropionitrile (DPN). The immunoprecipitation assay showed that NDRG2 could affect the complex components of ubiquitin-protein ligase E3A (UBE3A, known as E6AP) and ERβ, reducing the ubiquitin-mediated proteasome degradation of ERβ.

Conclusions: In the current study, we found that NDRG2 could bind with UBE3A to hinder the binding of UBE3A with ERβ. Moreover, a positive feedback loop was discovered between NDRG2 and ERβ, which provides a novel insight and therapeutic target for CRC.
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http://dx.doi.org/10.21037/jgo-20-557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807274PMC
December 2020

Bacterial seed endophyte shapes disease resistance in rice.

Nat Plants 2021 01 4;7(1):60-72. Epub 2021 Jan 4.

Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, China.

Cereal crop production is severely affected by seed-borne bacterial diseases across the world. Locally occurring disease resistance in various crops remains elusive. Here, we have observed that rice plants of the same cultivar can be differentiated into disease-resistant and susceptible phenotypes under the same pathogen pressure. Following the identification of a seed-endophytic bacterium as the resistance-conferring agent, integration of high-throughput data, gene mutagenesis and molecular interaction assays facilitated the discovery of the underlying mode of action. Sphingomonas melonis that is accumulated and transmitted across generations in disease-resistant rice seeds confers resistance to disease-susceptible phenotypes by producing anthranilic acid. Without affecting cell growth, anthranilic acid interferes with the sigma factor RpoS of the seed-borne pathogen Burkholderia plantarii, probably leading to impairment of upstream cascades that are required for virulence factor biosynthesis. The overall findings highlight the hidden role of seed endophytes in the phytopathology paradigm of 'disease triangles', which encompass the plant, pathogens and environmental conditions. These insights are potentially exploitable for modern crop cultivation threatened by globally widespread bacterial diseases.
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http://dx.doi.org/10.1038/s41477-020-00826-5DOI Listing
January 2021

Phenotype and genotype spectra of a Chinese cohort with nephronophthisis-related ciliopathy.

J Med Genet 2020 Dec 15. Epub 2020 Dec 15.

Department of Nephrology, Children's Hospital of Fudan University, Shanghai, China

Background: Nephronophthisis-related ciliopathies (NPHP-RC) account for the majority of cases of monogenetically caused end-stage renal disease (ESRD) in children. Exploring the correlation between the phenotype and genotype of NPHP-RC is helpful for early diagnosis and management. We investigated the phenotype and genotype spectra of NPHP-RC in a Chinese multicentre cohort.

Methods: Crosss-ectional and longitudinal data of 60 patients from 57 families with pathogenic NPHP-RC gene mutations distributed in 22 regions of China were collected into a unified, anonymous database. The mean observation time of this cohort was 3.5±3.1 years.

Results: Mutations in and were the most common genetic defects. Overall, 45% of patients presented with isolated nephronophthisis (NPH), and 55% exhibited the extrarenal phenotype, which frequently involved the liver (41.7%, n=25), central nervous system (26.7%, n=16), eyes (26.7%, n=16) and skeletal system (11.7%, n=7). Accidental detection of elevated serum creatinine and non-specific symptoms caused by chronic kidney disease occurred in 65% of patients. Patients carrying mutations mainly presented with isolated NPH (90%, 18/20) and progressed to ESRD at a mean age of 12.9±0.5 years. The mean age of ESRD onset in the non- group was lower than that in the group (6.2±1.4 years, p<0.001), especially for patients carrying mutations (3.1±1.2 years), showing a heterogeneous phenotype characterised by Bardet-Biedl syndrome (12.5%, n=5), Joubert syndrome (7.5%, n=3), COACH syndrome (2.5%, n=1), Mainzer-Saldino syndrome (2.5%, n=1), short-rib thoracic dysplasia (2.5%, n=1) and unclassified symptoms (32.5%, n=13).

Conclusions: The Chinese Children Genetic Kidney Disease Database registry characterised the spectrum of the phenotype and genotype of NPHP-RC in the Chinese population. and were the most common pathogenic genes. Rapid progression to ESRD and liver involvement were noted in patients with mutations.
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http://dx.doi.org/10.1136/jmedgenet-2020-107184DOI Listing
December 2020

Prevalence and associated factors of sexualized drug use in sex work among transgender women sex workers in China.

AIDS Care 2021 08 1;33(8):1098-1106. Epub 2020 Dec 1.

JC School of Public Health and Primary Care, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, People's Republic of China.

Sexualized drug use (SDU) is associated with a higher risk of HIV transmission. There is a dearth of study investigating the association between sex work characteristics and SDU in sex work among transgender women sex workers. To address these gaps, we conducted a secondary analysis of a cross-sectional study among 220 transgender women sex workers in Shenyang, China. The prevalence of SDU in sex work was 20.9% in the past month. After adjustment for age, HIV status, education, monthly income and duration of stay in Shenyang, performing sex work mainly in hotel rooms, charging higher per episode of commercial sex, depressive symptoms and anxiety symptoms were associated with higher likelihood of SDU in sex work. Future SDU prevention programmes SDU targeting transgender women sex workers should focus on those who charged higher and performed sex work in hotel rooms, and integrate mental health promotion as an essential component.
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http://dx.doi.org/10.1080/09540121.2020.1851017DOI Listing
August 2021

Role of homocysteine in the development and progression of Parkinson's disease.

Ann Clin Transl Neurol 2020 11 21;7(11):2332-2338. Epub 2020 Oct 21.

Department of Basic Medical Sciences, Taizhou University, Taizhou, China.

Homocysteine is an essential intermediate product of biochemical reactions that is present in various tissues of the human body. Homocysteine may be associated with the development and progression of Parkinson's disease. Plasma homocysteine levels in patients with Parkinson's disease are elevated compared to those of healthy individuals. High homocysteine drives PD development and progression while aggregating the clinical symptoms of PD patients. The relationship between PD and homocysteine involves multiple pathways, including nerve cell apoptosis, oxidative stress, and DNA damage. This is crucial for explaining how high homocysteine drives the PD procession. Elevated homocysteine level during PD development and progression offers a new strategy for the diagnosis and treatment of this disease.
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http://dx.doi.org/10.1002/acn3.51227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664283PMC
November 2020

Gut Microbiota Dysbiosis Drives the Development of Colorectal Cancer.

Digestion 2021 15;102(4):508-515. Epub 2020 Sep 15.

Department of Neurology, Taizhou Second People's Hospital, Taizhou, China,

Background: The gut microbiota is a diverse community of microbes that maintain the stability of the intestinal environment. Dysbiosis of the gut microbiota has been linked to gastrointestinal diseases, such as colorectal cancer (CRC) - a leading cause of death for cancer patients.

Summary: Candidate pathogens have been identified using bacterial culture and high-throughput sequencing techniques. Currently, there is evidence to show that specific intestinal microbes drive CRC development and progression, yet their pathogenic mechanisms are still unclear. Key Messages: In this review, we describe the known healthy gut microbiota and its changes in CRC. We especially focus on exploring the pathogenic mechanisms of gut microbiota dysbiosis in CRC. This is crucial for explaining how gut microbiota dysbiosis drives the process of colorectal carcinogenesis and tumor progression. Evaluation of changes in the gut microbiota during CRC development and progression offers a new strategy for the diagnosis and treatment of this disease.
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http://dx.doi.org/10.1159/000508328DOI Listing
August 2021

Insertion of a 1.9F central venous catheter via the internal jugular vein in neonates.

J Int Med Res 2020 Jun;48(6):300060520925380

Nursing Department, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Objective: This study aimed to develop a technique for placing a 1.9 French (F) central venous catheter in the internal jugular vein of newborns.

Methods: In this retrospective study, punctures were performed with a modified ultrasound-guided Seldinger technique with 57 1.9F catheters in 48 newborns. Punctures were performed in the right internal jugular vein in 43 (75.4%) patients and in the left internal jugular vein in 14 (24.6%) patients.

Results: We included 33 (57.9%) boys and 24 (42.1%) girls, aged a median 38 days (range, 2-135 days). The puncture success rate was 100%. Catheterization duration was a median 14 days (range, 1-70 days). Among the catheters, 94.1% were removed after completion of therapy or upon death. Fifty-three (93%) patients experienced no complication, whereas a small amount of bleeding was observed in 2 (3.5%) patients, inflammation of puncture in 1 (1.8%) patient, and occlusion in 1 (1.8%) patient. The method of placement of 1.9F catheters in the internal jugular vein of newborns had a high success rate, with minimal trauma and few complications.

Conclusions: Our method of placing a 1.9F central venous catheter in the internal jugular vein is suggested for level III to VI neonatal intensive care units.
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http://dx.doi.org/10.1177/0300060520925380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303769PMC
June 2020

Enhanced coagulation by two-stage alum addition: the role of solution pH, floc breakage and assistant of non-ionic polyacrylamide.

Environ Technol 2020 May 19:1-10. Epub 2020 May 19.

College of Architecture and Civil engineering, Beijing University of Technology, Beijing, People's Republic of China.

Coagulation process is highly influenced by solution pH, floc breakage, and coagulant aid. In this work, two-stage addition of alum-based coagulants, polyaluminum chloride (PACl) and aluminum sulfate (Al(SO)), was compared to their one-stage addition with the same total dosage. The dose ratio of primary and secondary addition was optimised. The effects of solution pH, floc breakage and dosing timing of coagulant aid of non-ionic polyacrylamide (NPAM) on coagulation efficiency and floc property were investigated. The results showed that two-stage addition of PACl showed less sensitivity with the variation of pH. Compared to PACl, two-stage addition of Al(SO) was more sensitive to the solution pH, especially when the pH ranged from 7 to 10. For all cases, the presence of floc breakage improved the removal of colloidal particles. The coagulation mechanism of primary coagulant was charge neutralisation, while other mechanism was involved with the secondary addition of coagulant. Compared to one-stage addition, the settling performance of aggregates and particulates removal in two-stage addition was more effective with the variation of dosage or dosing timing of NPAM. The present work provides detailed operating parameters for this coagulation strategy.
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http://dx.doi.org/10.1080/09593330.2020.1762757DOI Listing
May 2020

Effect of rapid-mixing conditions on the evolution of micro-flocs to final aggregates during two-stage alum addition.

Environ Technol 2021 Aug 9;42(20):3122-3131. Epub 2020 Feb 9.

College of Architecture and Civil Engineering, Beijing University of Technology, Chao Yang District, Beijing, People's Republic of China.

In a coagulation-flocculation process, optimal separation of the resultant aggregates plays a decisive role on coagulation performance and provides a lower burden for subsequent treatment units. This separation highly depends on the stability of the micro-flocs formed during the initial, rapid stage of coagulation. In this work, a two-stage addition of aluminium sulphate (alum, Al(SO)) was employed by adding 0.04 and 0.08 mM Al(SO) at the beginning and the end of rapid mixing, respectively. The coagulation performance and floc characteristics were compared to conventional single addition with the same total coagulant dosage, and the effects of variable rapid-mixing speeds (160-850 rpm) and duration time (10-120 s) were investigated. The results showed that the residual turbidity of two-stage coagulant addition was 85.1% lower than single addition when applied at a mixing speed of 580 rpm and a duration time of 120 s. The underlying coagulation mechanism revealed that the two-step addition more effectively neutralized colloids and formed larger aggregates that settled better and could more easily be removed. Moreover, the aggregates were less firm, which was attributed to different interactions among the micro-flocs, the second addition of Al(SO) and destabilized colloids. The present work provides data to broaden the window of rapid-mixing environments for more effective coagulation.
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http://dx.doi.org/10.1080/09593330.2020.1723710DOI Listing
August 2021

Evaluation of smartphone APP-based case-management services among antiretroviral treatment-naïve HIV-positive men who have sex with men: a randomized controlled trial protocol.

BMC Public Health 2020 Jan 20;20(1):85. Epub 2020 Jan 20.

Department of Medical Statistics, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, Guangdong, People's Republic of China.

Background: Men who have sex with men (MSM) are disproportionally affected by HIV in China. 'Treatment as Prevention' is a promising strategy for HIV prevention but requires adequate adherence. Mobile health (mHealth) may be an acceptable and feasible approach for service delivery, but there is little evidence supporting mHealth intervention for improving antiretroviral treatment adherence among HIV-infected MSM in low- and middle-income countries, including China. This study will aim to develop a smartphone application-based case-management service and compare its efficacy to standard care with regards to adherence, CD4, HIV viral load and psychosocial outcomes among MSM patients in Guangzhou, China.

Methods: A non-blinded 1:1 parallel-group randomised controlled trial will be conducted in Guangzhou Eighth People's Hospital, with 300 MSM enrolled in each arm. Eligible MSM who are newly initiating ART will be randomly assigned to an intervention group (standard-of-care case management plus mHealth intervention) or a control group (standard-of-care case management). The development of the mHealth intervention will be based on the information-motivation-behavioural skills theory of ART adherence, and comprise four components: educational articles, one-to-one online communication with case managers, support-service information and hospital-visit reminders. Outcome measures will be collected at baseline and at months 1, 3, 6, and 12. The primary outcomes will be ART adherence and CD4 count at month 6. Secondary outcomes include HIV RNA, sexual behaviours, mental health status, illness perceptions, and quality of life. χ test and t-test will be used for between-group comparisons. Intervention effects will be evaluated using General estimating equation performed by SAS 9.0, on the principle of intention-to-treat. Structural equation modelling will be used to test potential mechanisms of intervention effect.

Discussion: This study is the first to explore the efficacy of mHealth intervention in the case management services targeted at HIV-infected MSM in low-and middle-income countries. Once proven effective, the innovative mHealth service could be integrated into the routine case management of PLWH. as well as be tailored to the patient management service for other chronic conditions.

Trial Registration: ClinicalTrial.gov: NCT03860116; Registered on 1 March 2019.
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http://dx.doi.org/10.1186/s12889-020-8171-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971898PMC
January 2020

Double-layer substrate of shale ceramsite and active alumina tidal flow constructed wetland enhanced nitrogen removal from decentralized domestic sewage.

Sci Total Environ 2020 Feb 19;703:135629. Epub 2019 Nov 19.

College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, PR China. Electronic address:

This study explored the application of shale ceramsite (SC) overlaid onto active alumina (AA) to function as a double-layer substrate in tidal flow constructed wetland (TFCW, SC-AA-TFCW) for decentralized domestic sewage treatment. This was compared to AA or SC substrate alone (AA-TFCW or SC-TFCW) for nitrogen removal, variation of dissolved oxygen and porosity in TFCWs, as well as structure of bacterial communities at varied hydraulic load (HL) of 0.204-2.448 m/m d with time ratio of the wet and dry phase of 3:1. The results demonstrate that SC-AA-TFCW removed 86% NH-N and 79% total nitrogen at HL of 0.612 m/m d, which was better than AA-TFCW(76%) or SC-TFCW(49%). The higher nitrogen removal performance in SC-AA-TFCW was mainly attributed to enhanced oxygen transportation due to non-uniform flow field and irregular gap distribution in layered structure, as well as less pore blockage during long-term operation. Denitrifying bacteria including Dechloromonas, Acidovorax, Chryseobacterium and Thermomonas species took up 32% of the microbiome in SC-AA-TFCW, which was higher than in AA-TFCW (17%) and SC-TFCW (7.7%). This study highlighted the importance of layered structures and determined an optimal HL of TFCW to achieve an efficient and stable nitrogen removal for domestic sewage treatment.
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http://dx.doi.org/10.1016/j.scitotenv.2019.135629DOI Listing
February 2020

Effectiveness of a Psycho-Social Intervention Aimed at Reducing Attrition at Methadone Maintenance Treatment Clinics: A Propensity Score Matching Analysis.

Int J Environ Res Public Health 2019 11 7;16(22). Epub 2019 Nov 7.

Department of Medical Statistics, School of Public Health, Sun Yat-sen University, Guangzhou 510080, Guangdong, China.

Methadone maintenance treatment (MMT) is an important approach to address opioid dependence. However, MMT clinics usually report high attrition rates. Our previous randomized controlled trial demonstrated additional psycho-social services delivered by social workers could reduce attrition rates compared to MMT alone. This study aimed to evaluate the effectiveness of psycho-social service in a real-world context. A quasi-experimental design and propensity score matching was adopted. 359 clients were recruited from five MMT clinics in Guangzhou from July 2013 to April 2015. One 20-minute counseling session was offered to the control group after enrolment. The intervention group received six sessions of psycho-social services. The baseline characteristics were unbalanced between two arms in the original sample. After propensity score matching, 248 participants remained in the analysis. At month six, the intervention group had a lower attrition rate [intervention (39.5%) versus control (52.4%), = 0.041], higher monthly income [monthly income of 1000 CNY or higher: intervention (55.9%) versus control (39.0%), = 0.028)], higher detoxification intention score [full intention score: intervention (51.6%) versus control (32.5%), = 0.012)], higher family support in MMT participation [intervention (77.9%) versus control (61.4%), = 0.049)]. This study demonstrated that psycho-social services delivered by social workers can reduce MMT clients' attrition and improve their well-being in real-world settings.
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http://dx.doi.org/10.3390/ijerph16224337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888175PMC
November 2019

Microenvironmental Interplay Predominated by Beneficial Abates Fungal Pathogen Incidence in Paddy Environment.

Environ Sci Technol 2019 Nov 4;53(22):13042-13052. Epub 2019 Nov 4.

Sarawak Tropical Peat Research Institute , 94300 Kota Samarahan , Sarawak , Malaysia.

Rice fungal pathogens, responsible for severe rice yield loss and biotoxin contamination, cause increasing concerns on environmental safety and public health. In the paddy environment, we observed that the asymptomatic rice phyllosphere microenvironment was dominated by an indigenous fungus, , which positively correlated with alleviated incidence of , one of the most aggressive plant pathogens. Through the comparative metabolic profiling for the rice phyllosphere microenvironment, two metabolites were assigned as exclusively enriched metabolic markers in the asymptomatic phyllosphere and increased remarkably in a population-dependent manner with . These two metabolites evidenced to be produced by in either a phyllosphere microenvironment or artificial media were purified and identified as 2(3)-benzofuranone and azulene, respectively, by gas chromatography coupled to triple quadrupole mass spectrometry and nuclear magnetic resonance analyses. Combining with bioassay analysis in vivo and in vitro, we found that 2(3)-benzofuranone and azulene exerted dissimilar actions at the stage of infection-related development of . produced 2(3)-benzofuranone at the early stage to suppress gene expression, leading to inhibited mycelial growth, while azulene produced lately was involved in blocking of appressorium formation by downregulation of . More profoundly, the microenvironmental interplay dominated by significantly blocked epidemics in the paddy environment from 54.7 to 68.5% ( < 0.05). Our study first demonstrated implication of the microenvironmental interplay dominated by indigenous and beneficial fungus to ecological balance and safety of the paddy environment.
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http://dx.doi.org/10.1021/acs.est.9b04616DOI Listing
November 2019

CAR exosomes derived from effector CAR-T cells have potent antitumour effects and low toxicity.

Nat Commun 2019 09 25;10(1):4355. Epub 2019 Sep 25.

Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University, 200433, Shanghai, China.

Genetically engineered T cells expressing a chimeric antigen receptor (CAR) are rapidly emerging a promising new treatment for haematological and non-haematological malignancies. CAR-T therapy can induce rapid and durable clinical responses but is associated with unique acute toxicities. Moreover, CAR-T cells are vulnerable to immunosuppressive mechanisms. Here, we report that CAR-T cells release extracellular vesicles, mostly in the form of exosomes that carry CAR on their surface. The CAR-containing exosomes express a high level of cytotoxic molecules and inhibit tumour growth. Compared with CAR-T cells, CAR exosomes do not express Programmed cell Death protein 1 (PD1), and their antitumour effect cannot be weakened by recombinant PD-L1 treatment. In a preclinical in vivo model of cytokine release syndrome, the administration of CAR exosomes is relatively safe compared with CAR-T therapy. This study supports the use of exosomes as biomimetic nanovesicles that may be useful in future therapeutic approaches against tumours.
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http://dx.doi.org/10.1038/s41467-019-12321-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761190PMC
September 2019

MicroRNA-146a improves sepsis-induced cardiomyopathy by regulating the TLR-4/NF-κB signaling pathway.

Exp Ther Med 2019 Jul 10;18(1):779-785. Epub 2019 Jun 10.

Department of Nephrology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China.

The aim of the current study was to investigate the regulatory effect of miR-146a on the toll-like receptor 4 (TLR-4)/NF-κB pathway and therefore inflammation in septic cardiomyopathy. A total of 60 healthy male Sprague Dawley rats were equally divided into a control, LPS, miR-146a agonist and miR-146a inhibitor group. Blood samples were collected from rats 24 h after intraperitoneal lipopolysaccharide injection and myocardial tissues were subsequently collected. After hematoxylin and eosin staining of rat myocardial tissues, the degree of inflammatory cell infiltration and myocardial damage was observed. The content of certain myocardial injury markers were also observed, including cardiac troponin I (cTnI), B-type natriuretic peptide (BNP), creatine kinase myocardial bound (CK-MB) and myoglobin (Mb). Western blot analysis was performed to detect the expression of NF-κB/TLR-4, tumor necrosis factor (TNF-α) and intercellular adhesion molecule-1 (ICAM-1) in myocardial tissues. Reverse transcription-quantitative (RT-q) PCR was used to detect the expression of miR-146a, TNF-α, interleukin (IL)-1α and IL-1β mRNA in myocardial tissues. In the LPS group, myocardial interstitial tissue edema occurred, with enlarged and loosely arranged cardiomyocytes. Compared with the sepsis model group, myocardial interstitial tissue edema was relieved in the miR-146a agonist group, but was aggravated in the miR-146a inhibition group. The serum levels of cTnI, BNP, CK-MB, Mb, NF-κB, TLR-4, TNF-α and ICAM-1 in the sepsis model group were higher than those in the control group. In the miR-146a agonist group, levels of myocardial injury markers were lower than those in the sepsis model group, but were higher in the miR-146a inhibition group. The results of RT-qPCR demonstrated that the expression of miR-146a, TNF-α, IL-1α and IL-1β in the sepsis model group were upregulated compared with the control group. In addition, miR-146a expression in the miR-146a agonist group and the miR-146a inhibition group was increased, but TNF-α, IL-1α and IL-1β mRNA was downregulated. miR-146a may regulate the TLR-4/NF-κB signaling pathway via negative feedback mechanisms, leading to the improvement of the inflammatory response and cardiac dysfunction in sepsis-induced cardiomyopathy.
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http://dx.doi.org/10.3892/etm.2019.7657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591494PMC
July 2019

Adsorption characteristics of chloramphenicol onto powdered activated carbon and its desorption performance by ultrasound.

Environ Technol 2021 Jan 5;42(4):571-583. Epub 2019 Jul 5.

College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, People's Republic of China.

In this work, the adsorption characteristics, including adsorption kinetic, isotherm and thermodynamics of chloramphenicol (CAP) onto powdered activated carbon (PAC), were carried out, and the feasibility of using ultrasound to regenerate saturated PAC in aqueous solution was evaluated. The adsorption results demonstrated that the adsorption mechanism was a complex process including surface adsorption, external liquid membrane diffusion and intraparticle diffusion as well, and the adsorption process was endothermic and non-spontaneous. The optimal conditions for PAC regeneration were as follows: acoustic density of 0.36 W/mL, saturated carbon dosage of 1.0 g/L and pH value of 10.36. The analysis of thermogravimetric analysis (TGA), Fourier transform infrared spectrometry (FTIR), Brunauer-Emmett-Teller (BET) surface area, pore size distribution and scanning electron microscopy (SEM) of PAC samples revealed that ultrasound mainly acted on surface functionalities, meso-pores and macro-pores of PAC through hydroxyl radical oxidization, high-pressure shock waves and high-speed microjets due to cavitation effect. This study highlighted that ultrasound could be efficiently desorbing CAP from saturated PAC. Practical applications of this method for PAC regeneration with complex natural matrices under environmentally realistic conditions were conducted and the adsorption capacity of the regenerated PAC decreased.
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http://dx.doi.org/10.1080/09593330.2019.1637464DOI Listing
January 2021

Porous Nanofibers Formed by Heterogeneous Growth of ZnO/Ag Particles and the Enhanced Photocatalysis.

J Nanosci Nanotechnol 2019 Nov;19(11):7163-7168

College of Sino-German Science and Technology, Qingdao University of Science and Technology, Qingdao 266061, Shandong, P. R. China.

Porous ZnO/Ag nanofibers with unique multi-interface contact structures have been synthesized by an electrospinning method. The composite nanofibers with the length of several hundred micrometers are composed of uniformly distributed ZnO and Ag nanoparticles in the size range 10~30 nm. Notably, a heterogeneous growth in the interface of ZnO and Ag particles occurs. The MO degradation rate of ZnO/Ag nanofibers with Ag content of 18 wt.% is 100% in 10 min under UV-vis irradiation and that of ZnO/Ag nanofibers with Ag content of 15 wt.% is 92% in 40 min under simulated day light, which is two times that of the pure ZnO. It is attributed to a special structure that improves the generation and separation efficiency of the photo-generated charges by the expansion of the absorption edges, the utilization efficiency of the surface plasmon resonance (SPR) oscillations of Ag, and the effective transferring of electrons from ZnO through the heterogeneous-growth interface. No obvious loss of the photocatalytic activity is observed after five cycles, indicating the excellent photostability of ZnO/Ag nanofibers. The work sheds light on the structure design of highperformance composite photocatalysts.
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http://dx.doi.org/10.1166/jnn.2019.16518DOI Listing
November 2019

Efficacy of dendritic cell-based immunotherapy produced from cord blood in vitro and in a humanized NSG mouse cancer model.

Immunotherapy 2019 05;11(7):599-616

Department of Surgery, Hebei Medical University, 361 East Zhongshan Road,  Shijiazhuang 050017, China.

Aim: To produce dendritic cells (DCs) from CD34 stem cells from cord blood and explore their prophylactic and curative effect against tumors by vaccinating humanized NSG mice.

Materials & Methods: Separated CD34 stem cells from cord blood were cultured for 30 days, and the resultant DCs (CD34-DCs) were collected. The basic function of the CD34-DCs and the cytotoxicity of CD34-cytotoxic-T lymphocytes (CTLs) were tested in vitro, and tumor inhibition in a humanized NSG mouse tumor model was observed.

Results: The number of CD34-DCs reached approximately 9 log. These cells performed functions similar to those of DCs derived from monocytes from peripheral blood (PBMC-DCs). The CTLs of the CD34-DCs (CD34-CTLs) presented a better antitumor effect in vitro. The obvious prophylactic and therapeutic antitumor effects of the CD34-DC vaccine were observed in the humanized NSG mouse models.

Conclusion: CD34-DCs from cord blood were sufficient in quantity and quality as a vaccine agent against tumors in vitro and in vivo.
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http://dx.doi.org/10.2217/imt-2018-0103DOI Listing
May 2019

Targeting RyR2 with a phosphorylation site-specific nanobody reverses dysfunction of failing cardiomyocytes in rats.

FASEB J 2019 06 18;33(6):7467-7478. Epub 2019 Mar 18.

Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.

Chronic PKA phosphorylation of ryanodine receptor 2 (RyR2) has been shown to increase diastolic sarcoplasmic reticulum (SR) Ca leakage and lead to cardiac dysfunction. We hypothesize that intracellular gene delivery of an RyR2-targeting phosphorylation site-specific nanobody could preserve the contractility of the failing myocardium. In the present study, we acquired RyR2-specific nanobodies from a phage display library that were variable domains of heavy chain-only antibodies. One of the nanobodies, AR185, inhibited RyR2 phosphorylation and was chosen for further investigation. We investigated the potential of adeno-associated virus (AAV)9-mediated cardiac expression of AR185 to combat postischemic heart failure (HF). AAV gene delivery elevated the intracellular expression of the AR185 protein in a rat model of ischemic HF, and this treatment normalized the systolic and diastolic dysfunction of the failing myocardium by reversing myocardial Ca handling. Furthermore, AR185 gene transfer to failing cardiomyocytes reduced the frequency of SR calcium leaks, thereby restoring the attenuated intracellular calcium transients and SR calcium load. Moreover, AR185 gene transfer inhibited the PKA-mediated phosphorylation of RyR2 in failing cardiomyocytes. Our results provide preclinical experimental evidence that the cardiac expression of RyR2 nanobodies with AAV9 vectors is a promising therapeutic strategy for HF.-Li, T., Shen, Y., Lin, F., Fu, W., Liu, S., Wang, C., Liang, J., Fan, X., Ye, X., Tang, Y., Ding, M., Yang, Y., Lei, C., Hu, S. Targeting RyR2 with a phosphorylation site-specific nanobody reverses dysfunction of failing cardiomyocytes in rats.
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http://dx.doi.org/10.1096/fj.201802354RDOI Listing
June 2019

Enhanced simultaneous organics and nutrients removal in tidal flow constructed wetland using activated alumina as substrate treating domestic wastewater.

Bioresour Technol 2019 May 7;280:441-446. Epub 2019 Feb 7.

China Architecture Design & Research Group, Beijing 100044, China. Electronic address:

A tidal flow constructed wetland (TFCW), a commonly applied system to clean wastewater, contains a substrate to assist pollutants removal, while the choice of substrate affects the formation of bacterial biofilms. Herein, activated alumina-TFCW (A-TFCW) with hydraulic load of 1.35 m/(m·d) parallel with shale ceramisite (S-TFCW) was investigated for treating domestic wastewater, aiming to enhance simultaneous long-term removal of organics, nitrogen and phosphorus. A-TFCW achieved significantly higher COD, NH-N, TN and TP removal efficiency than S-TFCW, with the removal efficiency of 85.9% COD, 85.4% NH-N, 72.8% TN and 96.4% TP respectively. Denitrifying bacteria dominated in both formed biofilms, with higher relative abundance of nitrifying bacteria and denitrifying bacteria in A-TFCW. These results demonstrated that AA substrate was more suitable to be applied in enhancing the removal performance in TFCW for the treatment of domestic wastewater.
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http://dx.doi.org/10.1016/j.biortech.2019.02.036DOI Listing
May 2019

EGFR/Notch Antagonists Enhance the Response to Inhibitors of the PI3K-Akt Pathway by Decreasing Tumor-Initiating Cell Frequency.

Clin Cancer Res 2019 05 22;25(9):2835-2847. Epub 2019 Jan 22.

Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.

Purpose: Both EGFR and PI3K-Akt signaling pathways have been used as therapeutically actionable targets, but resistance is frequently reported. In this report, we show that enrichment of the cancer stem cell (CSC) subsets and dysregulation of Notch signaling underlie the challenges to therapy and describe the development of bispecific antibodies targeting both HER and Notch signaling.

Experimental Design: We utilized cell-based models to study Notch signaling in drug-induced CSC expansion. Both cancer cell line models and patient-derived xenograft tumors were used to evaluate the antitumor effects of bispecific antibodies. Cell assays, flow cytometry, qPCR, and serial transplantation assays were employed to investigate the mechanisms of action and pharmacodynamic readouts.

Results: We found that EGFR/Notch targeting bispecific antibodies exhibited a notable antistem cell effect in both and assays. Bispecific antibodies delayed the occurrence of acquired resistance to EGFR inhibitors in triple-negative breast cancer cell line-based models and showed efficacy in patient-derived xenografts. Moreover, the EGFR/Notch bispecific antibody PTG12 in combination with GDC-0941 exerted a stronger antitumor effect than the combined therapy of PI3K inhibitor with EGFR inhibitors or tarextumab in a broad spectrum of epithelial tumors. Mechanistically, bispecific antibody treatment inhibits the stem cell-like subpopulation, reduces tumor-initiating cell frequency, and downregulates the mesenchymal gene expression.

Conclusions: These findings suggest that the coblockade of EGFR and Notch signaling has the potential to increase the response to PI3K inhibition, and PTG12 may gain clinical efficacy when combined with PI3K blockage in cancer treatment.
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http://dx.doi.org/10.1158/1078-0432.CCR-18-2732DOI Listing
May 2019

Effect of marital relationship on women's quality of life: Testing the mediating role of subjective well-being.

J Community Psychol 2019 03 8;47(2):327-337. Epub 2018 Oct 8.

Hangzhou Normal University.

Because of a large number of Chinese women and the widespread concern about women's living conditions, this study aimed to examine how subjective well-being mediates the association between marital relationship and women's quality of life in Chinese context. The data used in this study were collected by conducting a multi-stage cluster random sampling method of 756 women in Huai'an city of Mainland China. Data included women's basic demographics, women's quality of life and marital quality, and women's subjective well-being. Structural equation modeling was adopted to analyze the data. The results of structural equation modeling analysis suggested a good fit for the sample. Overall findings showed that marital relationship has a significantly direct effect on women's quality of life, and subjective well-being can partially mediate the impact of marital relationship on women's quality of life. The implications on theory and social policy were also discussed.
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http://dx.doi.org/10.1002/jcop.22123DOI Listing
March 2019
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