Publications by authors named "Xiaoxiao Yu"

63 Publications

Activation of activator protein-1-fibroblast growth factor 21 signaling attenuates Cisplatin hepatotoxicity.

Biochem Pharmacol 2021 Dec 5;194:114823. Epub 2021 Nov 5.

Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, Queens, NY 11439, United States. Electronic address:

Fibroblast growth factor (Fgf/FGF) 21, which plays important roles in sugar, lipid and energy metabolism, has been accepted as a mito-stress marker gene. We recently reported that FGF21 expression can be up-regulated via activation of aryl hydrocarbon receptor (AhR) or glucocorticoid receptor (GR) and that FGF21 plays important cytoprotective roles. Cisplatin (cis-diamminedichloroplatinum, CDDP) is a widely used chemotherapeutic drug. Numerous adverse effects including hepatotoxicity have been noted during CDDP therapy. It is known that CDDP can induce mitochondrial dysfunction. The studies were designed to determine the regulation of Fgf/FGF21 expression by CDDP, and to characterize the underlying mechanisms of its regulation, as well as to determine the impact of gain or loss of Fgf/FGF21 function on the progression of CDDP hepatotoxicity. Our results showed that CDDP and phorbol ester induced mRNA and protein expression of Fgf/FGF21 and β-Klotho, two essential components of Fgf21 signaling, in mouse livers and cultured mouse/human hepatocytes. Luciferase reporter assays and ChIP-qPCR assays demonstrated that the cJun-AP-1 activation is responsible for CDDP- and phorbol ester-induced Fgf/FGF21 expression. Such induction is abolished after cotreated with AP-1 inhibitor SR11302. In addition, CDDP produces more severe liver injury in Fgf21-null than wild-type mice. Pre-treatment of GR activator dexamethasone or AhR activator β-Naphthoflavone, both of which can induce Fgf21 expression, attenuated CDDP-induced hepatotoxicity in vivo and in vitro. In conclusion, Fgf/FGF21-β-Klotho signaling can be activated via AP-1 activation. Gain of Fgf/FGF21 function attenuates the progression of CDDP hepatotoxicity, which may be considered clinically to improve CDDP therapy.
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http://dx.doi.org/10.1016/j.bcp.2021.114823DOI Listing
December 2021

Amide-bridged conjugated organic polymers: efficient metal-free catalysts for visible-light-driven CO reduction with HO to CO.

Chem Sci 2021 Sep 13;12(34):11548-11553. Epub 2021 Jul 13.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Colloid and Interface and Thermodynamics, CAS, Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences 100190 Beijing P. R. China

The visible-light-driven photoreduction of CO to value-added chemicals over metal-free photocatalysts without sacrificial reagents is very interesting, but challenging. Herein, we present amide-bridged conjugated organic polymers (amide-COPs) prepared self-condensation of amino nitriles in combination with hydrolysis, for the photoreduction of CO with HO without any photosensitizers or sacrificial reagents under visible light irradiation. These catalysts can afford CO as the sole carbonaceous product without H generation. Especially, amide-DAMN derived from diaminomaleonitrile exhibited the highest activity for the photoreduction of CO to CO with a generation rate of 20.6 μmol g h. Experiments and DFT calculations confirmed cyano/amide groups as active sites for CO reduction and second amine groups for HO oxidation, and suggested that superior selectivity towards CO may be attributed to the adjacent redox sites. This work presents a new insight into designing photocatalysts for artificial photosynthesis.
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http://dx.doi.org/10.1039/d1sc02499jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447930PMC
September 2021

Preparation and evaluation of humic acid-based composite dust suppressant for coal storage and transportation.

Environ Sci Pollut Res Int 2021 Oct 16. Epub 2021 Oct 16.

College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, Shandong, China.

To mitigate environmental pollution caused by the escape of dust during coal storage and transportation, humic acid (HA) and grafted acrylamide (AM) were used as raw materials to prepare a composite dust suppressant suitable for coal storage and transportation. Single-factor experiments were used to explore the optimal synthesis conditions of the dust suppressant, and the microstructure of the product was studied using Fourier-transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (H-NMR), scanning electron microscopy (SEM), and other methods. The wetting effect of the dust suppressant on coal was also investigated by way of molecular dynamics (MD) simulations. The experimental results showed that the dust suppressant had good wind erosion resistance (wind erosion rate 10.2%), shock resistance (loss rate 3.63%), and anti-evaporation performance, while the MD simulation and permeability analysis results showed that the dust suppressant had an excellent wetting effect on the coal surface. SEM images revealed that the dust suppressant can fill the gaps between coal dust particles and bond them together to form a consolidated layer, thereby effectively inhibiting the escape of dust sources during coal storage and transportation.
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http://dx.doi.org/10.1007/s11356-021-16685-2DOI Listing
October 2021

Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Feb 27;266:120410. Epub 2021 Sep 27.

Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002, Hubei, China.

In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite. This composite exhibited significantly enhanced oxidase-mimicking activity during catalyzing the oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB). The CS/Cu/Fe composite was comprehensively characterized and the possible catalytic mechanism was reasonably explored and discussed. Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic. Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg), the rapid colorimetric detection of Hg was successfully carried out in the analytical tube. This colorimetric method showed good linear response to Hg over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM. The method also revealed high selectivity and satisfactory results in recovery experiments of Hg detection in tap water and lake water. Furthermore, it was found that the effective removal of Hg could be realized in the analytical tube based on efficient Hg adsorption by CS/Cu/Fe composite and agarose hydrogel. This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg from contaminated water.
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http://dx.doi.org/10.1016/j.saa.2021.120410DOI Listing
February 2022

Dual recognition strategy for selective fluorescent detection of dopamine and antioxidants based on graphite carbon nitride in human blood serum.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jan 10;265:120385. Epub 2021 Sep 10.

Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002, China. Electronic address:

In this work, a strong blue-emitting fluorescent biosensor based on graphite carbon nitride nanoparticles (GCNNs) (E = 340 nm and E = 435 nm) was synthesized by a facile one-step hydrothermal method. With the aid of hydrogen peroxide and horseradish peroxidase, pyrocatechol structure of dopamine (DA) was oxidized to o-quinone structure of polydopamine (PDA) by hydroxyl radical. PDA was able to rapidly and significantly quench fluorescence of GCNNs. In the meanwhile, oxidative self-polymerization from DA to PDA would be blocked by antioxidants, such as glutathione (GSH) and ascorbic acid (AA). Thus, the fluorescence of [email protected] sensor would be recovered owing to the decrease of o-quinone. Based on above-mentioned dual recognition strategy of "turn-off" and "turn off-on", a fast, simple and ultrasensitive method was developed to measure DA and antioxidants. Under the optimal experimental conditions, the detection limits of DA, GSH and AA were 0.064 μmol L, 0.11 μmol L and 0.16 μmol L with relative standard deviations of 1.7%, 9.3% and 8.0%, respectively. As one of metal-free quantum dots, our GCNNs-based sensors were also successfully applied to the determination of DA as well as GSH and AA in human serum. The recoveries for the spiked samples were in the range of 93.8%-109% and 95.0%-110% of DA and antioxidants, which shows great promise to clinicalapplication.
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http://dx.doi.org/10.1016/j.saa.2021.120385DOI Listing
January 2022

Weak interaction-alleviated toxicity of aromatic compounds in EPS matrices: Quantifying the noncovalent bonding-to-EPS ecoservice chain.

J Hazard Mater 2021 08 20;416:125824. Epub 2021 Apr 20.

College of Resources and Environmental Sciences, Nanjing Agricultural University, Jiangsu 210095, China. Electronic address:

Extracellular polymeric substances (EPSs) constitute a largely global carbon pool that could participate in geochemical process of organic chemicals. Besides the chemical hydrolysis and redox of chemicals exerted by the EPS, weakly noncovalent interactions with dispersive EPS control the toxicity of numerous organic compounds. Nevertheless, there has been a lack of in-depth research on this issue. This work quantified a chain of links from bonding to detoxification using natural biofilms to explore the control behavior of fragile noncovalent bonding to the ecotoxicity of aromatic compounds. Such bonding decreases cell absorbability of m-phenylenediamine, 2-naphthol, and phenanthrene by 5.3-53.6%, resultantly increasing the indices of microbial diversity by 122.2-279.5%. Herein, the 60 kDa chaperonin in EPS acts as the most important contributor (16.4% of the top 20 proteins) to noncovalent interactions. Hydrophilic carboxyl groups in EPS bind with hydroxyl and amino groups of m-phenylenediamine and 2-naphthol via H-bonds, respectively. Methylene and carboxyl groups combine with hydrophobic phenanthrene via CH···π and H-bonding, respectively. A quantified chain was consequently established that weak interaction linearly controls ecotoxicity of aromatic compounds via the above suppressive cell absorbability of aromatic compounds (R =0.82). Considering ubiquitous EPS and prevailing aromatic compounds, our findings revealed a previously unnoticed phenomenon in which seemingly fragile noncovalent bonding profoundly alleviates the ecotoxicity of aromatic compounds in Earth's surface system.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125824DOI Listing
August 2021

Identification of a peptide binding to cancer antigen Kita-kyushu lung cancer antigen 1 from a phage-display library.

Cancer Sci 2021 Oct 23;112(10):4335-4345. Epub 2021 Aug 23.

The Comprehensive Cancer Center of Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Kita-kyushu lung cancer antigen 1 (KK-LC-1) is a kind of cancer-testis antigen with anti-tumor potential for clinical application. As a class of small-molecule antigen conjugate, tumor-targeting peptides have broad application prospects in gastric cancer diagnosis, imaging, and biological treatment. Here, we screened specific cyclic nonapeptides from a phage-display library. The targeting peptide with the best affinity was selected and further verified in ex vivo tissue sections. Finally, enrichment of targeting peptides in tumor tissues was observed in vivo, and the dynamic biodistribution process was also observed with micro-positron emission tomography (micro-PET)/computed tomography (CT) imaging. Studies showed that the specific cyclic nonapeptide had a high binding capacity for KK-LC-1 protein. It has a strong affinity and specificity for KK-LC-1-expressing positive tumor cells. Targeting peptides were significantly enriched at tumor sites in vivo, with very low normal tissue background. These findings demonstrated that the KK-LC-1 targeting peptide has high clinical potential.
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http://dx.doi.org/10.1111/cas.15109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486176PMC
October 2021

Multispectral Graphene-Based Electro-Optical Surfaces with Reversible Tunability from Visible to Microwave Wavelengths.

Nat Photonics 2021 Jul 5;15(7):493-498. Epub 2021 Apr 5.

Department of Materials, University of Manchester, Manchester, M13 9PL, UK.

Optical materials with colour-changing abilities have been explored for display devices, smart windows, or modulation of visual appearance. The efficiency of these materials, however, has strong wavelength dependence, which limits their functionality to a specific spectral range. Here, we report graphene-based electro-optical devices with unprecedented optical tunability covering the entire electromagnetic spectrum from the visible to microwave. We achieve this non-volatile and reversible tunability by electro-intercalation of lithium into graphene layers in an optically accessible device structure. This unique colour-changing capability, together with area-selective intercalation, inspires fabrication of new multispectral devices, including display devices and electro-optical camouflage coating. We anticipate that these results provide realistic approaches for programmable smart optical surfaces with a potential utility in many scientific and engineering fields such as active plasmonics and adaptive thermal management.
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http://dx.doi.org/10.1038/s41566-021-00791-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7611111PMC
July 2021

Identification of a Glypican-3 Binding Peptide From a Phage-Displayed Peptide Library for PET Imaging of Hepatocellular Carcinoma.

Front Oncol 2021 4;11:679336. Epub 2021 Jun 4.

The Comprehensive Cancer Centre of Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Tumor-targeting peptides functioned as molecular probes are essential for multi-modality imaging and molecular-targeting therapy in caner theronostics. Here, we performed a phage-displayed bio-panning to identify a specific binding peptide targeting Glypican-3 (GPC-3), a promising biomarker in hepatocellular carcinoma (HCC). After screening in the cyclic peptide library, a candidate peptide named F3, was isolated and showed specific binding to HCC cell lines. In a bio-distribution study, higher accumulation of F3 peptide was observed in HepG-2 tumors compared to PC-3 tumors in xenograft models. After labeling with radioactive Ga, the F3 peptide tracer enabled the specific detection of tumors in HCC tumor models with PET imaging. More importantly, the expression of GPC-3 in human tissue samples may be distinguished by an F3 fluorescent peptide probe indicating its potential for clinical application. This cyclic peptide targeting GPC-3 has been validated, and may be an alternative to serve as an imaging probe or a targeting domain in the drug conjugate.
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http://dx.doi.org/10.3389/fonc.2021.679336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212053PMC
June 2021

Bifunctional iRGD-anti-CD3 enhances antitumor potency of T cells by facilitating tumor infiltration and T-cell activation.

J Immunother Cancer 2021 05;9(5)

The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing, China

Background: Poor infiltration and limited activation of transferred T cells are fundamental factors impeding the development of adoptive cell immunotherapy in solid tumors. A tumor-penetrating peptide iRGD has been widely used to deliver drugs deep into tumor tissues. CD3-targeting bispecific antibodies represent a promising immunotherapy which recruits and activates T cells.

Methods: T-cell penetration was demonstrated in tumor spheroids using confocal microscope, and in xenografted tumors by histology and in vivo real-time fluorescence imaging. Activation and cytotoxicity of T cells were assessed by flow cytometry and confocal microscope. Bioluminescence imaging was used to evaluate in vivo antitumor effects, and transmission electron microscopy was used for mechanistic studies.

Results: We generated a novel bifunctional agent iRGD-anti-CD3 which could immobilize iRGD on the surface of T cells through CD3 engaging. We found that iRGD-anti-CD3 modification not only facilitated T-cell infiltration in 3D tumor spheroids and xenografted tumor nodules but also induced T-cell activation and cytotoxicity against target cancer cells. T cells modified with iRGD-anti-CD3 significantly inhibited tumor growth and prolonged survival in several xenograft mouse models, which was further enhanced by the combination of programmed cell death protein 1 (PD-1) blockade. Mechanistic studies revealed that iRGD-anti-CD3 initiated a transport pathway called vesiculovacuolar organelles in the endothelial cytoplasm to promote T-cell extravasation.

Conclusion: Altogether, we show that iRGD-anti-CD3 modification is an innovative and bifunctional strategy to overcome major bottlenecks in adoptive cell therapy. Moreover, we demonstrate that combination with PD-1 blockade can further improve antitumor efficacy of iRGD-anti-CD3-modified T cells.
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http://dx.doi.org/10.1136/jitc-2020-001925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126316PMC
May 2021

Quantitative analysis of 20 fentanyl analogues by modified QuEChERS-LC-MS/MS in health products and transdermal patches.

J Pharm Biomed Anal 2021 Jul 27;201:114100. Epub 2021 Apr 27.

Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, PR China. Electronic address:

The spreading of narcotics especially illicit novel psychoactive substances is a continuing problem in recent years. In response to reduce the morbidity and crime related to fentanyl analogues, the accurate measurement of fentanyl analogues concentrations is significantly important in the analytical laboratories for customs checks and clinical application. In this work, ethyl acetate was selected as extraction solvent, 50 mg of PSA, 100 mg of C18, and 10 mg of GCB were optimized for purification. A modified QuEChERS extraction method followed by high performance liquid chromatography-tandem mass spectrometry with the mode of multiple reaction monitoring has been developed for the simultaneous determination of 20 fentanyl analogues in collagen peptides, slimming capsules and fentanyl transdermal patches. The limits of detection (LODs) varied from 0.004 to 0.02 μg L with relative standard deviations of 4.89-11.4 % and showed good linearity in the range of 0.02-10 μg L and 0.01-1.00 mg L, respectively. The recoveries for 20 fentanyl analogues in the low (at μg L level) and high (at mg L level) concentration spiked samples were in the range of 77.7-114 % and 83.9-116 %, which demonstrated the application potential of the proposed method for the determination of fentanyl analogues with low and high concentration in real case samples. In addition, the matrix effect and the cross-reactivity were also proved to not interfere with quantitation of targeted fentanyl analogues. Thus, the developed method showed high sensitivity and good accuracy, which makes it suitable for the rapid detection of fentanyl analogues for customs and border service as well as pharmaceuticals.
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http://dx.doi.org/10.1016/j.jpba.2021.114100DOI Listing
July 2021

Foxq1 promotes metastasis of nasopharyngeal carcinoma by inducing vasculogenic mimicry via the EGFR signaling pathway.

Cell Death Dis 2021 04 19;12(5):411. Epub 2021 Apr 19.

Otorhinolaryngology Head and Neck Surgery, Nanfang Hospital of Southern Medical University, Guangzhou, China.

In nasopharyngeal carcinoma (NPC), the treatment of tumor metastasis and recurrence is challenging and is associated with poor clinical efficacy. Vasculogenic mimicry (VM) is a new blood-supply model of malignant tumor that is closely related to tumors' distant metastasis. Our previous study demonstrated that miR-124 could target Foxq1 to inhibit NPC metastasis. Whether Foxq1 affects metastasis through vasculogenic mimicry is worth consideration. In this study, we show that VM formation positively correlates with the expression of Foxq1, and EGFR, and the TNM stage in 114 NPC patient samples. Meanwhile, we show that VM-positive NPC patients have a poor prognosis. Furthermore, using in vitro and vivo approaches, we confirm that Foxq1 has a significant effect on NPC metastasis through promoting VM formation, which could be effectively inhibited by EGFR inhibitors (Nimotuzumab or Erlotinib). Also a synergistic efficacy of anti-EGFR and anti-VEGF drugs has been found in NPC inhibition. Mechanistically, the luciferase reporter gene and CHIP assays show that Foxq1 directly binds to the EGFR promoter region and regulates EGFR transcription. In conclusion, our results show that Foxq1 is regulated by miR-124 and that it promotes NPC metastasis by inducing VM via the EGFR signaling pathway. Overall, these results provide a new theoretical support and a novel target selection for anti-VM therapy in the treatment of nasopharyngeal carcinoma.
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http://dx.doi.org/10.1038/s41419-021-03674-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055972PMC
April 2021

Social support and posttraumatic stress disorder: A meta-analysis of longitudinal studies.

Clin Psychol Rev 2021 04 4;85:101998. Epub 2021 Mar 4.

School of Psychology, University of Queensland, Australia. Electronic address:

Social support has long been associated with posttraumatic stress disorder (PTSD), but there is no consistent evidence on the strength and direction of this relationship. Whereas the social causation model claims that social support buffers against PTSD, the social selection model states that PTSD reduces social support resources. As the first meta-analysis of the prospective relationships between social support and PTSD, this study synthesized the available longitudinal data (75 samples including 32,402 participants) on these two constructs with a random-effects model. In total, three hundred and fifty-five effect sizes (including cross-sectional, prospective and cross-lagged coefficients) were included in the meta-analysis. With prior levels of the relevant outcomes controlled for, results showed that social support and PTSD reciprocally predicted each other over time with similar effect sizes: Social support predicted PTSD with β = -0.10; PTSD predicted social support with β = -0.09. Moderator analyses suggested that the effects held across most sample characteristics and research designs except for several moderators (gender, time lag, publication year, source of support). These findings provided strong evidence for both the social causation and social selection models, suggesting that the link between social support and PTSD is symmetrically reciprocal and robust.
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http://dx.doi.org/10.1016/j.cpr.2021.101998DOI Listing
April 2021

Prospect of early vascular tone and satellite cell modulations on white striping muscle myopathy.

Poult Sci 2021 Mar 25;100(3):100945. Epub 2020 Dec 25.

State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. Electronic address:

Polyphasic myodegeneration potentially causes severe physiological and metabolic disorders in the breast muscle of fast-growing broiler chickens. To date, the etiology of recent muscle myopathies, such as the white striping (WS) phenotype, is still unknown. White striping-affected breast meats compromise the water holding capacity and predispose muscle to poor vascular tone, leading to the deterioration of meat qualities. Herein, this review article provides insight on the complexities around chicken breast myopathies: (i) the etiologies of WS occurrence in chicken; (ii) the metabolic changes that occur in WS defect in pectoralis major; and (iii) the interactions between breast muscle physiology and vascular tone. It also addressed the effects of nutritional supplements on muscle myopathies on chicken breast meats. Moreover, the review explored breast muscle biology focusing on the early preparation of satellite and vascular cells in fast-growth chicken breeds. Transcriptomics and histological analyses revealed poor vascularity in breast muscle of fast growth chickens. Thus, we suggest in ovo feeding of nutrients promoting vascularization and satellite cells replenishment as a potential strategy to enhance endothelium-derived nitric oxide availability to promote vascularization in the pectoralis major muscle region.
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http://dx.doi.org/10.1016/j.psj.2020.12.042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936185PMC
March 2021

Co-immobilization of multi-enzyme on reversibly soluble polymers in cascade catalysis for the one-pot conversion of gluconic acid from corn straw.

Bioresour Technol 2021 Feb 8;321:124509. Epub 2020 Dec 8.

College of Life Science, Jilin Agricultural University, Changchun 130118, China; The Key Laboratory of Straw Biology and Utilization, The Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address:

The difficulties in the process of cellulose cascade conversion based on immobilization technology lies in the recycling enzymes from rich solid-containing straw hydrolysate and the incompatibility of conventional immobilization with this process. In this study, three types of enzyme (cellulase, glucose oxidase and catalase) were successfully immobilized on a reversible soluble Eudragit L-100. Through the determination of the preparation conditions, enzymatic properties and catalytic conditions, the co-immobilized enzyme was applied to the catalytic reaction of one-pot conversion of corn straw to gluconic acid. The yield of gluconic acid achieved 0.28 mg/mg, conversion rate of cellulose in corn straw to gluconic acid reached 61.41%. The recovery of co-immobilized enzyme from solid substrate was achieved by using reversible and soluble characteristics of the carrier. After 6 times of recycling, the activity of co-immobilized enzyme was maintained at 52.38%, confirming the feasibility of multi-enzyme immobilization strategy using reversible soluble carrier in cascade reactions.
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http://dx.doi.org/10.1016/j.biortech.2020.124509DOI Listing
February 2021

Enantiomeric Switching of the Circularly Polarized Luminescence Processes in a Hierarchical Biomimetic System by Film Tilting.

ACS Nano 2021 01 4;15(1):1397-1406. Epub 2020 Dec 4.

Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study and Development of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.

Circularly polarized luminescence (CPL) switching has attracted great attention due to the potential applications in chiral photonics and electronics. However, the lack of examples to achieve switchable CPL within a single material in the dry solid state hampers the scope of applications. Herein, we demonstrate a crystalline chiral polymer film as a polarizing medium consisting of radially assembled twisted crystallites, where achiral aggregation-induced emissive luminogens (AIEgens) are confined between the twisted crystalline stacks, eventually yielding handedness-switchable CPL by simple film tilting. Hierarchically organized twisted crystallites create the selective reflection activity of the polarizing medium. Upon film tilting, enantiomeric switching is realized by selectively collecting transmitted and reflected CPL components. The confined AIEgens in the crystalline polarizing system show a great enhancement of the luminescence efficiency. Moreover, the approach is general with broadband activity, and various AIEgens could be applied to generate full-color-tunable CPL. Additionally, the rigid and continuous nature of this polarizing system affords enhanced optical stability and facile modulation, developing a general route for designing chiroptical materials.
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http://dx.doi.org/10.1021/acsnano.0c08665DOI Listing
January 2021

Recent advances in single-cell analysis by inductively coupled plasma-mass spectrometry: A review.

Anal Chim Acta 2020 Nov 24;1137:191-207. Epub 2020 Jul 24.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China. Electronic address:

The content, migration and transformation of trace elements in living organisms play an important role in physiological activities. The analysis of trace elements in cells is of great significance to understand the function mechanism of trace elements in cells and organisms. Compared with conventional bulk analysis, many medical and clinical researches benefit from cell heterogeneity via single-cell analysis. Time-resolved analysis-inductively coupled plasma-mass spectrometry (TRA-ICP-MS) based strategies have the potential for both counting and quantitative assessment. It has been used to evaluate cell-to-cell difference in elemental content, cellular uptake of metal-containing drugs and nanoparticles. This review focuses on TRA-ICP-MS based methodologies for single-cell analysis over the past few decades. Recent advances in ICP-MS instrumentations (cell introduction systems and mass spectrometers), microfluidic platforms and their in-depth applications in single-cell analysis are summarized. This review also prospects future developments of TRA-ICP-MS for single-cell analysis.
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http://dx.doi.org/10.1016/j.aca.2020.07.041DOI Listing
November 2020

Argon Enclosed Droplet Based 3D Microfluidic Device Online Coupled with Time-Resolved ICPMS for Determination of Cadmium and Zinc in Single Cells Exposed to Cadmium Ion.

Anal Chem 2020 10 16;92(19):13550-13557. Epub 2020 Sep 16.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.

Time-resolved (TRA)-ICPMS has become a booming subfield of single-cell analysis tools in recent years, while generation of single cells remains the major challenge. Microfluidic devices reveal their great capability and potential in encapsulation of single cells into water droplets. However, current strategies to pinch off droplets require a specific oil phase, which is not compatible to conventional ICPMS and makes the signal of cells in the water phase susceptible. Herein, we built a 3D water-in-gas microfluidic device (3D W/G MFD) with commercially available components, producing single cell droplet enclosed by argon gas. By simply tuning the flow rate of gas and water, the droplets were generated to encapsulate single cells, which significantly reduced the probability of the single signal coming from multiple cells by 1 or 2 orders of magnitude compared to direct injection. The developed oil-free 3D W/G MFD was more friendly to online coupling with TRA-ICPMS than water-in-oil devices. The effect of Cd on HepG2 cells was studied by single cell detecting total Zn with 3D W/G MFD-TRA-ICPMS, and the variation of labile Zn was explored by flow cytometry with an N-(6-methoxy-8-quinolyl)-p-toluenesulfonamide probe. To the best of our knowledge, this work pioneered the exploration of variation in cellular metal content and speciation at the single-cell level, compensating for the deficiency of speciation analysis based on TRA-ICPMS and providing new insights into exploring the complexity of biology.
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http://dx.doi.org/10.1021/acs.analchem.0c03194DOI Listing
October 2020

Photocatalytic Reduction of CO to CO over Quinacridone/BiVO Nanocomposites.

ChemSusChem 2020 Oct 4;13(20):5565-5570. Epub 2020 Sep 4.

Beijing National Laboratory for Molecular Sciences Key Laboratory of Colloid, Interface and Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

Solar energy-driven photoreduction of CO to energy-rich chemicals is of significance for sustainable development but challenging. Herein, quinacridone (QA)/nBiVO (n=0.2-20, in which n stands for the mass ratio of BiVO to QA) nanocomposites were developed for photoreduction of CO . Characterization of the materials with Fourier-transform (FT)IR spectroscopy and X-ray photoelectron spectroscopy (XPS) pointed to QA/nBiVO preparation via hydrogen-bonding-directed self-assembly of QA on BiVO nanosheets. Using triethanolamine (TEOA) as a sacrifice reagent, QA/10BiVO showed the best performance, affording CO with a production rate of 407 μmol g  h , 24 times higher than those of pure QA. It was indicated that the Z-scheme charge-transfer mechanism of QA/nBiVO could significantly improve the separation and transmission efficiency of photo-generated electrons and holes. This novel approach provides new insight for fabricating the composite photocatalytic materials of small molecule organic semiconductors and inorganic semiconductors with high efficiency for photocatalytic of reduction CO .
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http://dx.doi.org/10.1002/cssc.202001764DOI Listing
October 2020

Recent history of metal contamination in the Fangcheng Bay (Beibu Gulf, South China) utilizing spatially-distributed sediment cores: Responding to local urbanization and industrialization.

Mar Pollut Bull 2020 Sep 13;158:111418. Epub 2020 Jul 13.

First Institute of Oceanology, Ministry of Natural Resources, Qingdao 266061, China.

In this study, the recent history of heavy metal pollution in the Fangcheng Bay (South China) was reconstructed utilizing three Pb-dated sediment cores. The metal concentration profiles display three trends since the 1970s and clearly reflect local urbanization and industrialization. The metals in the Fangcheng Bay started to accumulate in the 1970s but remained relatively low until the 1990s which corresponds to the slow urbanization and industrialization. The metal accumulation in the eastern Fangcheng Bay peaked in the early 2000s following the steep increases in accordance with the rapid industrialization of the eastern Fangcheng Bay where the core HSL was collected. Conversely, the heavy metal profiles in the western Fangcheng Bay present slight step increases in the early 2000s followed by a dramatic metal enrichment in the late 2000s; the expansion of these two cores, which begins in the early 2000s, concurs well with the rapid local urbanization and industrialization.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111418DOI Listing
September 2020

Enhancing Thermal Interface Conductance to Graphene Using Ni-Pd Alloy Contacts.

ACS Appl Mater Interfaces 2020 Jul 16;12(30):34317-34322. Epub 2020 Jul 16.

Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States.

To identify superior thermal contacts to graphene, we implement a high-throughput methodology that systematically explores the Ni-Pd alloy composition spectrum and the effect of Cr adhesion layer thickness on thermal interface conductance with monolayer graphene. Frequency domain thermoreflectance measurements of two independently prepared Ni-Pd/Cr/graphene/SiO samples identify a maximum metal/graphene/SiO junction thermal interface conductance of 114 ± (39, 25) MW/m K and 113 ± (33, 22) MW/m K at ∼10 at. % Pd in Ni-nearly double the highest reported value for pure metals and 3 times that of pure Ni or Pd. The presence of Cr, at any thickness, suppresses this maximum. Although the origin of the peak is unresolved, we find that it correlates with a region of the Ni-Pd phase diagram that exhibits a miscibility gap. Cross-sectional imaging by high-resolution transmission electron microscopy identifies striations in the alloy at this particular composition, consistent with separation into multiple phases. Through this work, we draw attention to alloys in the search for better contacts to two-dimensional materials for next-generation devices.
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http://dx.doi.org/10.1021/acsami.0c06953DOI Listing
July 2020

Exonuclease III-Powered Self-Propelled DNA Machine for Distinctly Amplified Detection of Nucleic Acid and Protein.

Anal Chem 2020 07 26;92(14):9764-9771. Epub 2020 Jun 26.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao 266042, China.

Herein, a new exonuclease III (Exo III)-powered self-propelled DNA machine was developed for the cascade multilevel signal amplification of nucleic acid and nucleic acid-related analytes. It could be easily and homogeneously operated with the use of an integral DNA hybrid probe as the recognition, amplification, and signaling element, and the Exo III cleavage as a driving force. The DNA hybrid probe was obtained by annealing two hairpin-like DNAs. The target recognition with the 3'-protruding domain of the DNA hybrid probe triggered Exo III cleavage, accompanied by target recycling and alternate generation of a large amount of target substitute and analogy. Simultaneously, the cascade bidirectional Exo III cleavage toward the DNA hybrid probe by the generated target substitute and analogy contributed for the exponential signal amplification toward target recognition event. It could be also extended for the application in protein detection with the thrombin as a protein example by introducing an additional hairpin-like aptamer switch. The proposed Exo III-powered self-propelled DNA amplification strategy showed a linear detection range for target DNA from 0.5 fM to 1 pM and for thrombin from 5 fM to 10 pM. The low detection limit toward target DNA and thrombin could reach about 0.1 fM and 5 fM, respectively, which were superior to most of reported methods. It also exhibited an excellent selectivity toward target detection. Therefore, the developed sensing system exhibits a new, simple and powerful means for amplified detection of nucleic acid and nucleic acid-related analytes, and may hold great potentials in bioanalysis, disease diagnosis and biomedicine.
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http://dx.doi.org/10.1021/acs.analchem.0c01197DOI Listing
July 2020

Direct Z-Scheme Heterojunction of SnS /Sulfur-Bridged Covalent Triazine Frameworks for Visible-Light-Driven CO Photoreduction.

ChemSusChem 2020 Dec 15;13(23):6278-6283. Epub 2020 May 15.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Colloid, Interface and Thermodynamics, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P.R. China.

Solar-driven reduction of CO into renewable carbon forms is considered as an alternative approach to address global warming and the energy crisis but suffers from low efficiency of the photocatalysts. Herein, a direct Z-Scheme SnS /sulfur-bridged covalent triazine frameworks (S-CTFs) photocatalyst (denoted as SnS /S-CTFs) was developed, which could efficiently adsorb CO owing to the CO -philic feature of S-CTFs and promote separation of photoinduced electron-hole pairs. Under visible-light irradiation, SnS /S-CTFs exhibited excellent performance for CO photoreduction, yielding CO and CH with evolution rates of 123.6 and 43.4 μmol g  h , respectively, much better than the most catalysts reported to date. This inorganic/organic hybrid with direct Z-Scheme structure for visible-light-driven CO photoreduction provides new insights for designing photocatalysts with high efficiency for solar-to-fuel conversion.
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http://dx.doi.org/10.1002/cssc.202000712DOI Listing
December 2020

MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47.

Biomed Res Int 2020 20;2020:3723781. Epub 2020 Feb 20.

Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Nasopharyngeal carcinoma (NPC) causes severe oncogenic lesions in the nasopharynx. CD47, a transmembrane integrin-associated protein, plays a key role in the ability of tumor cells to escape phagocytosis, working as an immune checkpoint in the immune response. Besides this role, CD47 has been reported to regulate cell proliferation and migration. The present study addresses the relationship between CD47 and microRNA-200a and examines their regulatory mechanisms in NPC. Bioinformatics analyses and dual-luciferase reporter assays were used to confirm the putative relationship between miR-200a and CD47, and their interaction was further detected using western blotting and RT-PCR. Further, results showed that miR-200a affect NPC cell proliferation, migration, and invasion by regulating CD47. A cell phagocytosis assay showed that miR-200a and a CD47 monoclonal antibody increased the sensitivity of NPC cells to macrophage phagocytosis by inhibiting the functions of CD47. Additionally, miR-200a expression was suppressed and CD47 expression increased in both clinical NPC tissues and cell lines. Taken together, these results show the miR-200a/CD47 combination as a potential therapeutic for treatment of NPC.
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http://dx.doi.org/10.1155/2020/3723781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054800PMC
December 2020

Characterization of the Active Components of the Multimerized sTNFRIIAdiponectin Fusion Protein Showing Both TNFα-Antagonizing and Glucose Uptake-Promoting Activities.

Endocr Metab Immune Disord Drug Targets 2020 ;20(7):1081-1089

Zhejiang Provincial Key Laboratory of Technology & Application of Model Organisms, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.

Background: The sTNFRII-adiponectin fusion protein previously showed strong TNFα antagonistic activity. However, the fusion protein exists as mixture of different multimers. The aim of the present study was to characterize its active components.

Methods: In this study, the fusion protein was isolated and purified by Ni-NTA affinity and gel exclusion chromatography, and further identified by Coomassie staining and western blotting. The TNFα antagonistic and glucose uptake-promoting activities were determined in vitro. The glucose detection kit and 2- NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose) were used to measure their effects on glucose metabolism (including glucose consumption and glucose uptake in HepG2 and H9C2 cells). The effect of the fusion protein on glucose uptake was also examined in free fatty acid (FFA)- induced insulin resistance cell model.

Results: The sTNFRII-adiponectin fusion protein was found to exist in three forms: 250 kDa (hexamer), 130 kDa (trimer), and 60 kDa (monomer), with the final purity of 90.2%, 60.1%, and 81.6%, respectively. The fusion protein could effectively antagonize the killing effect of TNFα in L929 cells, and the multimer was found to be superior to the monomer. In addition, the fusion protein could increase glucose consumption without impacting the number of cells (HepG2, H9C2 cells) in a dosedependent manner. Mechanistically, glucose uptake was found to be enhanced by the translocation of GLUT4. However, it could not improve glucose uptake in the cell model of insulin resistance.

Conclusion: In summary, the active components of the fusion protein are hexamers and trimers. The hexamer and trimer of sTNFRII-adiponectin fusion protein had both TNFα-antagonizing and glucose uptake-promoting activities, although neither of them could improve glucose uptake in the cell model of insulin resistance.
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http://dx.doi.org/10.2174/1871530320666200121100449DOI Listing
June 2021

Structures of lipoprotein signal peptidase II from Staphylococcus aureus complexed with antibiotics globomycin and myxovirescin.

Nat Commun 2020 01 9;11(1):140. Epub 2020 Jan 9.

Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, D02 R590, Ireland.

Antimicrobial resistance is a major global threat that calls for new antibiotics. Globomycin and myxovirescin are two natural antibiotics that target the lipoprotein-processing enzyme, LspA, thereby compromising the integrity of the bacterial cell envelope. As part of a project aimed at understanding their mechanism of action and for drug development, we provide high-resolution crystal structures of the enzyme from the human pathogen methicillin-resistant Staphylococcus aureus (MRSA) complexed with globomycin and with myxovirescin. Our results reveal an instance of convergent evolution. The two antibiotics possess different molecular structures. Yet, they appear to inhibit identically as non-cleavable tetrahedral intermediate analogs. Remarkably, the two antibiotics superpose along nineteen contiguous atoms that interact similarly with LspA. This 19-atom motif recapitulates a part of the substrate lipoprotein in its proposed binding mode. Incorporating this motif into a scaffold with suitable pharmacokinetic properties should enable the development of effective antibiotics with built-in resistance hardiness.
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http://dx.doi.org/10.1038/s41467-019-13724-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952399PMC
January 2020

PolyI:C Upregulated CCR5 and Promoted THP-1-Derived Macrophage Chemotaxis via TLR3/JMJD1A Signalling.

Cell J 2020 Oct 15;22(3):325-333. Epub 2019 Dec 15.

Department of Paediatrics, Qilu Hospital of Shandong University, Jinan, Shandong, China. Electronic Address

Objective: This study aimed to evaluate the specific roles of polyinosinic:polycytidylic acid (polyI:C) in macrophage chemotaxis and reveal the potential regulatory mechanisms related to chemokine receptor 5 ().

Materials And Methods: In this experimental study, THP-1-derived macrophages (THP1-Mφs) induced from THP- 1 monocytes were treated with 25 μg/mL polyI:C. Toll-like receptor 3 (), Jumonji domain-containing protein (JMJD)1A, and small interfering RNA (siRNAs) were transfected into THP1-Mφs. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was used to detect the expression levels of , , 23 Jumonji C domain-containing histone demethylase family members, , and in THP1-Mφs with different siRNAs transfections. Western blot was performed to detect , JMJD1C, H3K9me2, and H3K9me3 expressions. A transwell migration assay was conducted to detect THP1-Mφ chemotaxis toward chemokine ligand 3 (CCL3). A chromatin immunoprecipitation (ChIP) assay was performed to detect H3K9me2-CCR5 complexes in THP1- Mφs.

Results: PolyI:C significantly upregulated in THP1-Mφs and promoted chemotaxis toward CCL3 (P<0.05); these effects were significantly inhibited by siRNA (P<0.01). JMJD1A and JMJD1C expression was significantly upregulated in polyI:C-stimulated THP1-Mφs, while only JMJD1A siRNA decreased expression (P<0.05). JMJD1A siRNA significantly increased H3K9me2 expression in THP1-Mφs but not in polyI:C-stimulated THP1-Mφs. The ChIP result revealed that polyI:C significantly downregulated H3K9me2 in the promoter region of CCR5 in THP1- Mφs.

Conclusion: PolyI:C can enhance THP1-Mφ chemotaxis toward CCL3 regulated by /JMJD1A signalling and activate expression by reducing H3K9me2 in the promoter region of .
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http://dx.doi.org/10.22074/cellj.2020.6713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947015PMC
October 2020

Chemical Reactions Impede Thermal Transport Across Metal/β-GaO Interfaces.

Nano Lett 2019 12 2;19(12):8533-8538. Epub 2019 Dec 2.

Department of Mechanical Engineering , Carnegie Mellon University , Pittsburgh , Pennsylvania 15213 , United States.

The impact of chemical reactions on the thermal boundary conductance (TBC) of Au/metal contact/β-GaO layered samples as a function of contact thickness is investigated using high-throughput thermoreflectance measurements. A maximum in TBC of 530 ± 40 (260 ± 25) MW/m K is discovered for a Cr (Ti) contact at a thickness of 2.5 (5) nm. There is no local maximum for a Ni contact, for which the TBC saturates at 410 ± 35 MW/m K for thicknesses greater than 3 nm. Relative to the Au/β-GaO interface, which has a TBC of 45 ± 7 MW/m K, these nanoscale contacts enhance TBC by factors of 6 to 12. The TBC maximum only exists for metals capable of forming oxides that are enthalpically favorable compared to β-GaO. The formation of CrO, via oxygen removal from the β-GaO substrate, is confirmed by TEM analysis. The reaction-formed oxide layer reduces the potential TBC and leads to the maximum, which is followed by a plateau at a lower value, as its thickness saturates due to passivation. Many advanced materials are prone to similar chemical reactions, impacting contact engineering and thermal management for a variety of applications.
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http://dx.doi.org/10.1021/acs.nanolett.9b03017DOI Listing
December 2019

Co-Immobilization of Tri-Enzymes for the Conversion of Hydroxymethylfurfural to 2,5-Diformylfuran.

Molecules 2019 Oct 10;24(20). Epub 2019 Oct 10.

Key Laboratory of Straw Biology and Utilization, The Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130118, China.

Acting as a "green" manufacturing route, the enzyme toolbox made up of galactose oxidase, catalase, and horseradish peroxidase can achieve a satisfactory yield of 2,5-diformylfuran derived from 30 mM hydroxymethylfurfural. However, as the concentration of hydroxymethylfurfural increases, the substrate causes oxidative damage to the activity of the tri-enzyme system, and the accumulated hydrogen peroxide produced by galactose oxidase causes tri-enzyme inactivation. The cost of tri-enzymes is also very high. These problems prevent the utilization of this enzyme toolbox in practice. To address this, galactose oxidase, catalase, and horseradish peroxidase were co-immobilized into Cu(PO) nanoflowers in this study. The resulting co-immobilized tri-enzymes possessed better tolerance towards the oxidative damage caused by hydroxymethylfurfural at high concentrations, as compared to free tri-enzymes. Moreover, the 2,5-diformylfuran yield of co-immobilized tri-enzymes (95.7 ± 2.7%) was 1.06 times higher than that of separately immobilized enzymes (90.4 ± 1.9%). This result could be attributed to the boosted protective effect provided by catalase to the activity of galactose oxidase, owing to the physical proximity between them on the same support. After 30 recycles, co-immobilized tri-enzymes still achieves 86% of the initial yield. Moreover, co-immobilized tri-enzymes show enhanced thermal stability compared with free tri-enzymes. This work paves the way for the production of 2,5-diformylfuran from hydroxymethylfurfural via co-immobilized tri-enzymes.
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http://dx.doi.org/10.3390/molecules24203648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832383PMC
October 2019

A rose bengal-functionalized porous organic polymer for carboxylative cyclization of propargyl alcohols with CO.

Chem Commun (Camb) 2019 Oct 30;55(83):12475-12478. Epub 2019 Sep 30.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Colloid, Interface and Thermodynamics, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. and University of Chinese Academy of Sciences, Beijing 100049, China.

A Rose bengal-functionalized porous organic polymer (RB-POP) was prepared with a specific surface area of up to 562 m g. In the presence of 1,8-diazabicyclo[5.4.0]undec-7-ene, RB-POP supported Ag(0) nanoparticles exhibited excellent performance for catalyzing cyclization of propargyl alcohols with CO at 30 °C, achieving a TOF of 5000 h, the highest value among the reported ones.
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http://dx.doi.org/10.1039/c9cc07043eDOI Listing
October 2019
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