Publications by authors named "Xiaowen Wang"

320 Publications

Caveolin-1 Deficiency Induces Atrial Fibrosis and Increases Susceptibility to Atrial Fibrillation by the STAT3 Signaling Pathway.

J Cardiovasc Pharmacol 2021 Aug;78(2):175-183

Departments of Cardiology; and.

Abstract: Atrial fibrillation (AF) is a common arrhythmia in the clinic. Ablation failure and recurrence after cardioversion have become medical problems worldwide. An important pathological feature of AF is atrial fibrosis, which increases susceptibility to AF. As an important target of fibrosis signal integration, the signal transducer and activator of transcription 3 (STAT3) signaling pathway plays an important role in fibrosis. Caveolin-1 (CAV1), a cell membrane protein, is involved in a variety of the biological functions of cells. However, the role of CAV1 in atrial fibrosis remains unclear. In this study, Masson's trichrome staining was used to detect the degree of atrial fibrosis, and the expression of CAV1 in the human atrium was evaluated by immunohistochemistry. To further study the role of CAV1, its expression in cultured rat atrial fibroblasts was silenced using siRNAs. Atrial fibroblasts were treated with angiotensin II to observe the effects on CAV1 and the transforming growth factor-β1 and STAT3 signaling pathways. We also detected the effects of CAV1 scaffolding domain (CSD) peptide on fibrosis through the addition of exogenous CSD peptide. The results showed that CAV1 expression decreased with the aggravation of atrial fibrosis and that this effect increased the incidence of AF. The depletion of CAV1 induced excessive extracellular matrix deposition by activating the STAT3 and transforming growth factor-β1/SMAD2 signaling pathways, and this effect was exacerbated by stimulation with angiotensin II and improved by CSD peptide. These data suggested that CAV1 not only plays a critical role in fibrosis progression but also provides a target for the treatment of atrial fibrosis and AF.
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http://dx.doi.org/10.1097/FJC.0000000000001066DOI Listing
August 2021

An IL-1β homologue induced inflammation and antibacterial immune defense in Siberian sturgeon (Acipenser baeri).

Fish Shellfish Immunol 2021 Sep 16;118:283-293. Epub 2021 Sep 16.

Beijing Fisheries Research Institute & Beijing Key Laboratory of Fishery Biotechnology, Beijing, 100068, People's Republic of China; National Freshwater Fisheries Engineering Technology Research Center, Beijing, 100068, People's Republic of China. Electronic address:

Interleukin-1β is a key pro-inflammatory cytokine functioning in initiation of inflammatory responses against bacterial- and viral-infections. In the present study, a putative IL-1β counterpart was identified from Siberian sturgeon (Acipenser baeri) and designated as AbIL-1β. The Abil-1β cDNA sequence consists of 1130 bp with an open reading frame (ORF) of 585 bp, which encodes a 194 amino acid (aa) protein. Multiple amino acid sequence alignment revealed that a possible mature peptide could start at Leu, although no cut site for ICE (IL-1β converting enzyme) enzyme was present in Siberian sturgeon IL-1β. Even if AbIL-1β shares a relative low identity (33.6%) with another sturgeon type II IL-1β gene from Acipenser dabryanus, they still clustered together in phylogenetic tree. Endogenous Abil-1β was highly expressed in brain, blood, head kidney and spleen of healthy Siberian sturgeon, and remarkably up regulated in head kidney, spleen, and liver upon Aeromonas hydrophila (A.h) challenge. Consistently, in vitro stimulation test using heat-killed A.h and LPS significantly increased Abil-1β transcripts of primary spleen cells. To investigate the bactericidal capability of AbIL-1β, recombinant AbIL-1β (rAbIL-1β) was generated by prokaryotes. Pre-injection of rAbIL-1β reduced the bacterial load in sturgeon spleen after A.h infection. Further, rAbIL-1β was served as feed additive and demonstrated to enhance hybrid sturgeon's defense against A.h infection by increased expressional levels of immune-related genes (IL-1β, IL-6, IL-8, IgM and MHCIIβ), elevated activities of serum lysosome, ACH50, and MPO, as well as higher percent survival. In summary, the current results suggested that AbIL-1β functions in immune regulation and could improve sturgeon's resistance to bacterial infection.
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http://dx.doi.org/10.1016/j.fsi.2021.08.030DOI Listing
September 2021

Having hobbies and the risk of cardiovascular disease incidence: A Japan public health center-based study.

Atherosclerosis 2021 Sep 3;335:1-7. Epub 2021 Sep 3.

Department of Preventive Cardiology, National Cerebral and Cardiovascular Center, Suita, Japan.

Background And Aims: The role of hobbies as a protective factor against cardiovascular diseases (CVD) has gained increasing attention; however, no large-scale studies were performed to confirm this. We aimed to examine the association between having hobbies and the risk of total CVD, coronary heart disease (CHD), and stroke in a large Japanese cohort.

Methods: A total of 56,381 adults aged 45-74 years were divided into the non-hobby, having a hobby, and having many hobbies groups. We performed Cox proportional hazard models to estimate the hazard ratio (HRs) for incident CVD, CHD, and stroke after adjusting for potential confounding factors.

Results: We identified 3685 incident CVDs (940 CHDs and 2839 strokes) during a median follow-up of 16.3 years. After multivariable adjustment, compared to the non-hobby group, participants having a hobby and many hobbies had a 10% (HR = 0.90 [0.83, 0.97]) and 20% (HR = 0.80 [0.69, 0.93]) lower risk of CVD incidence, respectively. The risk of CHD was lower in those with many hobbies, but the association between having many hobbies and CHD risk was not statistically significant. We also found a similar inverse association for stroke. Compared to the non-hobby group, participants having a hobby and many hobbies had a 13% (HR = 0.87 [0.80, 0.96]) and 20% (HR = 0.80 [0.68, 0.94]) lower risk of stroke.

Conclusions: Having hobbies was associated with a lower risk of CVD. Engagement in hobbies may emerge as an important target for healthy lifestyle promotion for the primary prevention of CVD.
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http://dx.doi.org/10.1016/j.atherosclerosis.2021.09.002DOI Listing
September 2021

Cutaneous tuberculosis in a patient with a STAT1 mutation.

J Dtsch Dermatol Ges 2021 Sep 13. Epub 2021 Sep 13.

Department of Dermatology and Venereology, Peking University First Hospital, Research Center for Medical Mycology, Peking University, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, National Clinical Research Center for Skin and Immune Diseases, Beijing, China.

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http://dx.doi.org/10.1111/ddg.14576DOI Listing
September 2021

A Facile and Scalable Method of Fabrication of Large-Area Ultrathin Graphene Oxide Nanofiltration Membrane.

ACS Nano 2021 Sep 3. Epub 2021 Sep 3.

Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hong Kong SAR 999077, China.

With their ultrafast water transport and excellent molecule sieving properties, graphene oxide (GO)-based membranes show great potential in the membrane filtration field for water purification and molecular separation. However, the inability of uniform GO membranes to be produced on an industrial scale and their nonenvironmentally friendly reduction treatment are the bottleneck preventing their industrial applications. Herein, we report a scalable ultrathin uniform GO membrane fabrication technique. Ultrathin GO membranes with a large area of 30 × 80 cm and a thickness of a few nanometers were uniformly and facilely fabricated using a continuous process combining Mayer rod-coating and a short-time, high-power UV reduction. The interlayer spacing of the GO membrane could be effectively reduced and regulated to improve the salt rejection rate. The fabricated membrane showed superior water permeability of over 60.0 kg m h and a high separation efficiency of over 96.0% for a sodium sulfate (NaSO) solution. It also exhibited excellent mechanical stability under various harsh crossflow conditions. More importantly, the fabrication method developed here can be scaled up using a roll-to-roll industrial production process, which successfully solves the problem currently faced by GO membrane researchers and makes the industrial usage of GO membrane a reality.
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http://dx.doi.org/10.1021/acsnano.1c06155DOI Listing
September 2021

Mediterranean Diet and Mortality in People with Cardiovascular Disease: A Meta-Analysis of Prospective Cohort Studies.

Nutrients 2021 Jul 29;13(8). Epub 2021 Jul 29.

Public Health, Department of Social Medicine, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.

The association of the Mediterranean diet (MD) with mortality among people with a history of cardiovascular disease (CVD) has not been systematically examined. Hereby, our objective was to investigate the association of MD with all-cause and cardiovascular mortality in people with a history of CVD. We searched five electronic databases including Embase, PubMed, Scopus, Web of Science, and Cochrane Central Register of Controlled Trials to screen eligible studies published before 31 August 2020. A random-effect model was used to examine the association of a 2-unit increment in MD score with the risk of all-cause and cardiovascular mortality. We conducted sensitivity and subgroup analyses and examined potential publication bias by Egger's and Begg's tests. Seven cohort studies (eight datasets) with a total of 37,879 participants who had a history of CVD were eligible for the main analysis. The pooled hazard ratios were 0.85 (95% CIs: 0.78-0.93; n = 8) for all-cause mortality and 0.91 (95% CIs; 0.82-1.01; n = 4) for cardiovascular mortality for each 2-unit increment in a score of adherence to MD. Subgroup analyses for all-cause mortality showed that the association appeared relatively stronger in Mediterranean areas (HR = 0.76 [0.69-0.83]) than non-Mediterranean areas (HR = 0.95 [0.93-0.98]) and in studies with a shorter duration (HR = 0.75 [0.66-0.84] for <7 years vs. HR = 0.94 [0.91-0.98] for ≥7 years). No evidence of publication bias was observed. The present meta-analysis of prospective cohort studies provided evidence that adherence to MD improved survival in people with a history of CVD.
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http://dx.doi.org/10.3390/nu13082623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400615PMC
July 2021

Dietary Soy Consumption and Cardiovascular Mortality among Chinese People with Type 2 Diabetes.

Nutrients 2021 Jul 23;13(8). Epub 2021 Jul 23.

Public Health, Department of Social Medicine, Osaka University Graduate School of Medicine, Osaka 5650871, Japan.

Randomized controlled trials showed that soy intervention significantly improved blood lipids in people with diabetes. We sought to prospectively examine the association of soy consumption with the risk of cardiovascular death among individuals with diabetes. A total of 26,139 participants with a history of diabetes were selected from the Chinese Kadoorie Biobank study. Soy food consumption was assessed by a food frequency questionnaire. Causes of death were coded by the 10th International Classification of Diseases. The Cox proportional hazard regression was used to compute the hazard ratios. During a median follow-up of 7.8 years, a total of 1626 deaths from cardiovascular disease (CVD) were recorded. Compared with individuals who never consumed soy foods, the multivariable-adjusted risks (95% confidence intervals) of CVD mortality were 0.92 (0.78, 1.09), 0.89 (0.75, 1.05), and 0.77 (0.62, 0.96) for those who consumed soy foods monthly, 1-3 days/week, and ≥4 days/week, respectively. For cause-specific cardiovascular mortality, significant inverse associations were observed for coronary heart disease and acute myocardial infarction. Higher soy food consumption was associated with a lower risk of cardiovascular death, especially death from coronary heart disease and acute myocardial infarction, in Chinese adults with diabetes.
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http://dx.doi.org/10.3390/nu13082513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398979PMC
July 2021

Trends and Patterns of Knee Osteoarthritis in China: A Longitudinal Study of 17.7 Million Adults from 2008 to 2017.

Int J Environ Res Public Health 2021 08 23;18(16). Epub 2021 Aug 23.

School of Nursing, Peking University, No. 38 Xueyuan Road, Beijing 100191, China.

Knee osteoarthritis (KOA) provides many challenges on the healthcare system. However, few studies have reported the epidemiology, particularly in a large population. Our study aimed to estimate the prevalence, incidence, trends, and patterns of diagnosed KOA in China. This was a longitudinal study. We used health insurance claims of 17.7 million adults from 2008-2017 to identify people with KOA. Trends in prevalence and incidence were analyzed using joinpoint regression. We identified 2,447,990 people with KOA in Beijing, 60% of which were women. The 10-year average age-standardized prevalence and incidence of KOA was, respectively, 4.6% and 25.2 per 1000 person-years. Prevalence increased with age, surging after 55 years old. The average crude prevalence was 13.2% for people over 55 years old. The prevalence showed an increasing trend from 2008 to 2017, including a period of rapid rise from 2008 to 2011 ( < 0.05); the increase in prevalence was greatest in people under 35 years old ( 0.05). Our analyses showed that the annual prevalence rate of KOA increased significantly from 2008 to 2017 in China. We need to increase our attention to women and the elderly over 55 years old, and also be alert to the younger trend of incidence of KOA.
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http://dx.doi.org/10.3390/ijerph18168864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395063PMC
August 2021

Benign prostatic hyperplasia and cardiovascular risk: a prospective study among Chinese men.

World J Urol 2021 Aug 23. Epub 2021 Aug 23.

Public Health, Department of Social Medicine, Osaka University Graduate School of Medicine, Osaka, 5650871, Japan.

Purpose: To examine the prospective association of BPH with subsequent risk of CVD, including heart disease and stroke.

Methods: We used data from China Health and Retirement Longitudinal Study of 5242 Chinese men aged 45 years or older. During a follow-up of 7 years, we identified 613 cases of non-fatal CVD, including 417 heart diseases and 254 strokes. Cox proportional hazards models yielded hazard ratios (HRs) relating BPH to CVD, heart disease, and stroke incidence.

Results: Overall, 8.1% of men reported a history of BPH diagnosed by physicians at baseline. As compared with men without a history of BPH, those reporting a history of BPH had an increased risk of developing CVD (multivariable-adjusted HR = 1.43 [1.12, 1.83], heart disease (1.35 [1.00, 1.83]), and stroke (1.50 [1.03, 2.19]). The subgroup analysis by age at baseline (< 60 vs. ≥ 60 years) showed that the associations appeared to be evident among men < 60 years, particularly for CVD (1.82 [1.24, 2.69]) and heart disease (1.72 [1.06, 2.79]). However, interaction tests suggested the associations were not significantly modified by age (P for interaction > 0.10 for all outcomes).

Conclusion: In Chinese men, BPH was associated with higher risks of CVD, heart disease and stroke, particularly among men aged < 60 years.
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http://dx.doi.org/10.1007/s00345-021-03817-zDOI Listing
August 2021

Meal frequency and incidence of type 2 diabetes: a prospective study.

Br J Nutr 2021 Aug 23:1-6. Epub 2021 Aug 23.

Public Health, Department of Social Medicine, Osaka University Graduate School of Medicine, Osaka5650871, Japan.

Dietary habits play an important role in the development of obesity and type 2 diabetes. However, evidence on association between diet frequency and type 2 diabetes was limited and inconclusive. We aimed to examine the association between meal frequency and risk of type 2 diabetes. The cohort study used data from China Health and Retirement Longitudinal Study of 8874 community-dwelling people aged over 45 years. Participants were classified as eating two meals per day, three meals per day and four meals per day. Multiple Poisson regression models were used to examine risk of 4-year incident type 2 diabetes among people who ate more or less than three meals per day compared with people who ate three meals per day. We documented 706 type 2 diabetes cases during follow-up. After adjustment for known risk factors for type 2 diabetes, except for BMI, participants who ate four meals per day were at a lower risk of type 2 diabetes than those who ate three meals per day (relative risk(RR) = 0·73 (0·58, 0·92)). After further adjustment for baseline BMI, the association was slightly attenuated but remained statistically significant (RR = 0·76 (0·60, 0·97)). Subgroup analysis showed that the fully adjusted RR of type 2 diabetes for people eating four meals per day were 0·66 (0·48, 0·91) and 0·93 (0·65, 1·34) among those had a BMI < 25 and ≥ 25 kg/m2, respectively. Eating four meals per day, compared with eating three meals per day was associated with lower risk of developing type 2 diabetes in a Chinese population, particularly in those with a BMI < 25 kg/m2.
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http://dx.doi.org/10.1017/S0007114521003226DOI Listing
August 2021

A novel β-1,3-glucanase Gns6 from rice possesses antifungal activity against Magnaporthe oryzae.

J Plant Physiol 2021 Oct 10;265:153493. Epub 2021 Aug 10.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences of Nanjing Agricultural University, 210095, Nanjing, PR China; Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address:

As members of the pathogenesis-related protein (PR)-2 family, β-1,3-glucanases play pivotal roles in plant defense. Previous study showed that the rice genome contains 16 genes encoding putative β-1,3-glucanases, and the β-1,3-glucanases in subfamily A were deduced to be involved in plant defense. However, there was limited direct evidence. In this study, the expression of rice β-1,3-glucanases Gns2-Gns6 belonging to subfamily A in rice plant infection with Magnaporthe oryzae was investigated, and the enhanced expression of Gns6 during infection confirmed its crucial role in the defense of rice seedlings. Enzymological characterization revealed that Gns6 preferentially hydrolyzed laminarin, pachymaran, and yeast glucan. The β-1,3; 1,6-glucanase Gns6 exhibited a specific activity of 1.2 U/mg with laminarin as the substrate. In addition, Gns6 could hydrolyze laminarin via an endo-type mechanism, yielding a series of oligosaccharides with various degrees of polymerization that are known immune elicitors in plants. Moreover, Gns6 exhibited a significant inhibitory effect against the formation of the germ tubes and appressoria, with potential applications in plant protection. Taken together, this study shows that Gns6 is an essential effector in the defensive response of rice against pathogenic fungi.
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http://dx.doi.org/10.1016/j.jplph.2021.153493DOI Listing
October 2021

Functional Characterization of the Novel Laminaripentaose-Producing β-1,3-Glucanase GluB and Its Biocontrol of .

J Agric Food Chem 2021 Aug 11;69(33):9571-9584. Epub 2021 Aug 11.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences of Nanjing Agricultural University, Nanjing 210095, P. R. China.

Fungal cell wall synthesizing enzymes or remodeling enzymes represent key factors for the interaction of plant pathogen and antifungal agents, which are regarded as potential biocontrol agents. In this study, a novel endo-β-1,3-glucanase from was expressed and characterized. The expression of GluB was significantly upregulated after 2 days of liquid culture and 48 h after infection, indicating that it may be involved in cell wall reconstitution. Purified GluB exhibited high activity on insoluble β-glucans, with a specific activity of 8.18 U/mg toward yeast glucan at pH 9.0 and 50 °C. GluB hydrolyzed pachymaran and yeast glucan into oligosaccharides dominated by laminaripentaose, suggesting that it is an endo-β-1,3-glucanase. Incubation of 8 μg of GluB with 10 spores/mL resulted in the inhibition of conidial germination and appressorium formation of , illustrating effective biocontrol activity. Hydrolysates of pachymaran induced the expression of defense genes restricting infection in rice plants, indicating an immunostimulatory effect of GluB hydrolysates.
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http://dx.doi.org/10.1021/acs.jafc.1c03072DOI Listing
August 2021

The role of personality, social economic and prevention strategy effects on health-related quality of life among people living with HIV/AIDS.

Infect Dis Poverty 2021 Aug 3;10(1):104. Epub 2021 Aug 3.

Yunnan Center for Disease Control and Prevention, No.158, Dongsi Street, Xishan Municipal, Kunming, Yunnan Province, China.

Background: HIV/AIDS has transformed into a chronic controllable but not yet curable infectious disease as other chronic diseases to some extent. The additional of so called fourth 90% that included the improved health-related quality of life (HRQoL) for people living with HIV (PLWHIV) required solutions beyond antiretroviral therapy and viral load suppression. This study will explore the role of personality, social economic and prevention strategy effection on HRQoL among people living with HIV/AIDS.

Methods: A cross-sectional study was conducted among PLWHIV aged more than 16 years old in the 10 municipalities in Yunnan Province, China from October 2019 to May 2020, enrolling total 1997 participants. Individual-level HRQoL data were measured by 12-item Short Form Health Survey (SF-12) and EuroQol Five Dimensions Questionnaire (EQ-5D-5L). We assembled municipal-level data about social economic from Yunnan Statistical Yearbook in 2020 and strategy practice information from the self-evaluation system. We used the principal component analysis to build the social economic and strategy effect on each area respectively and one-way ANOVA was used to perform univariate analysis to identify the predictors with significant differences. Finally we used multi-level model (MLM) to explore the personality, social economic and strategy effects in health-related quality of life among PLWHIV.

Results: The global score for quality of life measured using EQ-5D-5L had an estimated mean score (standard deviation, SD) of 0.901 ± 0.146. The HRQoL score measured using PCS-12 had an estimated mean score (SD) of 46.62 ± 8.55. The mean MCS-12 score (SD) was estimated to be 47.80 ± 9.71. The area-level predictors explained a proportion of 13.6-17.2% for the between-area variation of the HRQoL scores, regardless of the total HRQoL, physical component and mental component. The impacts of stigma (P < 0.01), social support (P < 0.001), anxiety (P < 0.001), depression (P < 0.05) and social economic status (P < 0.05) on HRQoL at the individual-level were significantly different. The plots visualized the impact of individual-level factors on a respondent's HRQoL was modified by the area-level characteristics.

Conclusions: The study identified the possible strategy determinant of individual HRQoL of PLWHIV and also the area effect on HRQoL. Stigma, social support, anxiety, depression and social economic status were the individual-level determinants on HRQoL. These could be a valuable resource for evaluating the overall health of the areas and help improve local decision making.
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http://dx.doi.org/10.1186/s40249-021-00890-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336408PMC
August 2021

Cyclodextrin-based dispersive liquid-liquid microextraction for the determination of fungicides in water, juice, and vinegar samples via HPLC.

Food Chem 2022 Jan 22;367:130664. Epub 2021 Jul 22.

College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China; Graduate Education Innovation Center on Baijiu Bioengineering in Shanxi Province, Taigu, Shanxi 030801, PR China. Electronic address:

Cyclodextrin-based dispersive liquid-liquid microextraction (CD-DLLME) was developed for the determination of triazole and strobilurin fungicides in water, juice, and vinegar samples using high-performance liquid chromatography-diode-array detection (HPLC-DAD). Undecanol, which is a green solvent, was selected as the extraction solvent. A cyclodextrin aqueous solution was chosen as the dispersion solvent and demulsifier to avoid the use of a toxic dispersion solvent and eliminate the centrifugation step. Dispersion and phase separation were completed within 1 and 60 s, respectively. The linear range of this method was 1 to 100 µg L. The limits of detection were 0.3 μg L along with the preconcentration factor of 133 and enrichment factor of 124. The recovery was 83.2% to 103.2%. This pretreatment method was fast, simple, and environmentally friendly and was successfully applied to the analysis of triazole and strobilurin fungicide residues in water, juice, and vinegar samples.
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http://dx.doi.org/10.1016/j.foodchem.2021.130664DOI Listing
January 2022

Curcumin enhances the membrane trafficking of the sodium iodide symporter and augments radioiodine uptake in dedifferentiated thyroid cancer cells suppression of the PI3K-AKT signaling pathway.

Food Funct 2021 Sep 20;12(18):8260-8273. Epub 2021 Sep 20.

NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, 214063, China.

Radioactive iodine (RAI) is commonly used to treat differentiated thyroid cancer (DTC). A major challenge is the dedifferentiation of DTC with the loss of radioiodine uptake. Patients with distant metastases have persistent or recurrent disease and develop resistance to RAI therapy due to tumor dedifferentiation. Hence, tumor redifferentiation to restore sensitivity to RAI therapy is considered a promising strategy to overcome RAI resistance. In the present study, curcumin, a natural polyphenolic compound, was found to re-induce cell differentiation and increase the expression of thyroid-specific transcription factors, TTF-1, TTF-2 and transcriptional factor paired box 8 (PAX8), and iodide-metabolizing proteins, including thyroid stimulating hormone receptor (TSHR), thyroid peroxidase (TPO) and sodium iodide symporter (NIS) in dedifferentiated thyroid cancer cell lines, BCPAP and KTC-1. Importantly, curcumin enhanced NIS glycosylation and its membrane trafficking, resulting in a significant improvement of radioiodine uptake . Additionally, AKT knockdown phenocopied the restoration of thyroid-specific gene expression; however, ectopic expressed AKT inhibited curcumin-induced up-regulation of NIS protein, demonstrating that curcumin might improve radioiodine sensitivity the inhibition of the PI3K-AKT-mTOR signaling pathway. Our study demonstrates that curcumin could represent a promising adjunctive therapy for restoring iodide avidity and improve radioiodine therapeutic efficacy in patients with RAI-refractory thyroid carcinoma.
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http://dx.doi.org/10.1039/d1fo01073eDOI Listing
September 2021

Sociodemographic Disparities in Outpatient Cardiology Telemedicine During the COVID-19 Pandemic.

Circ Cardiovasc Qual Outcomes 2021 08 23;14(8):e007813. Epub 2021 Jul 23.

Cardiology Division (R.B.W., J.H.W.), Massachusetts General Hospital, Harvard Medical School, Boston.

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http://dx.doi.org/10.1161/CIRCOUTCOMES.121.007813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366531PMC
August 2021

The role of chronic suppressive antibiotics therapy in superficial drive line infection relapse of left ventricular assist devices: A retrospective cohort from a tertiary care center.

Transpl Infect Dis 2021 Aug 20;23(4):e13686. Epub 2021 Jul 20.

Division of Infectious Diseases, Washington University School of Medicine, St Louis, Missouri, USA.

Introduction: Drive line infections (DLIs) are common complications of left ventricular assist devices (LVADs). Data on use of suppression antibiotic therapy are limited.

Methods: We performed a retrospective review of 451 patients who underwent LVAD placement from January 2009 to May 2015. First superficial DLIs were included for analysis. We examined factors associated with the use of chronic suppressive antibiotics (CSAs) therapy. Cox proportional hazards models were performed to identify factors associated with DLI relapse with the same organism as the initial DLI.

Results: A total of 69 patients developed a superficial DLI within a median of 195 (interquartile range [IQR] 98-348) days of LVAD insertion. The median age was 57 years, 87% were males, and 74% were White. Gram positive bacteria caused 61% of infections, with Staphylococcus aureus being the most common (35%). Forty-three (62%) patients received suppressive antibiotic therapy. Relapse DLI occurred in 29 (42%) patients. Independent risk factors for relapse infection in multivariable analysis were sepsis (aHR 5.94 [CI 1.42-24.92]), and MRSA DLI (aHR 4.19 [CI 1.37-12.79]). There was no difference in the proportion of patients with relapse among those who were treated with antibiotic suppression therapy versus not (44% vs. 38%, p = 0.64), although relapse occurred at a later time in those who received suppression (185 vs. 69 days, p < 0.01).

Conclusion: CSA therapy was associated with delayed time to DLI relapse but no significant difference in the proportion of patients with relapse. A prospective study is needed to examine the effect of suppression on relapse rates.
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http://dx.doi.org/10.1111/tid.13686DOI Listing
August 2021

Overexpression of OsPHR3 improves growth traits and facilitates nitrogen use efficiency under low phosphate condition.

Plant Physiol Biochem 2021 Sep 24;166:712-722. Epub 2021 Jun 24.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Phosphorus (P) and nitrogen (N) are both essential macronutrients for maintaining plant growth and development. In rice (Oryza sativa L.), OsPHR3 is one of the four paralogs of PHR1, which acts as a central regulator of phosphate (Pi) homeostasis, as well being involved in N homeostasis. However, the functions of OsPHR3 in N utilization under different Pi conditions have yet to be fully studied. In this study, we aimed to dissect the effect of OsPHR3-overexpression on N utilization under Pi deficient regimes. Biochemical, molecular and physiological assays were performed to determine the N-influx, translocation, and accumulation in OsPHR3-overexpressing rice lines, grown under Pi-sufficient and -deficient conditions, in both hydroponic and soil systems. Furthermore, important agronomic traits of these plants were also evaluated. The overexpression of OsPHR3 increased N uptake under Pi stress regimes. Increased N uptake also elevated total N concentrations in these plants by inducing N transporter genes expression. Furthermore, overexpression of OsPHR3 increased N use efficiency, 1000-grain weight and grain yield under different Pi conditions. We established new findings that OsPHR3-overexpression facilitates N utilization under Pi deficient conditions. This will help achieving higher yields by coordinating the utilization of N and P.
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http://dx.doi.org/10.1016/j.plaphy.2021.06.041DOI Listing
September 2021

Disseminated trichosporosis in a young patient with CARD9 deficiency.

Clin Microbiol Infect 2021 Jun 16. Epub 2021 Jun 16.

Department of Dermatology, Peking University First Hospital, Research Center for Medical Mycology, Peking University, National Clinical Research Center for Skin and Immune Diseases, Beijing, China. Electronic address:

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http://dx.doi.org/10.1016/j.cmi.2021.06.012DOI Listing
June 2021

Cutaneous and subcutaneous fungal infections: recent developments on host-fungus interactions.

Curr Opin Microbiol 2021 08 4;62:93-102. Epub 2021 Jun 4.

Department of Dermatology and Venerology, Peking University First Hospital, China; Research Center for Medical Mycology, Peking University, China; Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, China; National Clinical Research Center for Skin and Immune Diseases, Beijing 100034, China. Electronic address:

The incidence of skin fungal infections is increasing at an alarming rate worldwide, presenting a major challenge to health professionals. Cutaneous and subcutaneous fungal infections are caused by pathogenic or opportunistic organisms varying from mold, yeasts, to dimorphic fungi. Recently, skin fungal have been increasingly reported and studied, giving rise to crucial breakthroughs in etiology and pathogenesis. This review aims to summarize recent insights into the clinical and etiological characteristics of common skin fungal infections according to different fungal species, as well as remarkable advances in the immune mechanisms. We hope it will be helpful to understand these diverse skin fungal infections, and bring about the latest developments that may facilitate novel diagnostic and therapeutic approaches to improve the outcomes in these patients.
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http://dx.doi.org/10.1016/j.mib.2021.05.005DOI Listing
August 2021

Necroptosis pathway blockage attenuates PFKFB3 inhibitor-induced cell viability loss and genome instability in colorectal cancer cells.

Am J Cancer Res 2021 15;11(5):2062-2080. Epub 2021 May 15.

Key Laboratory of Precision Oncology of Shandong Higher Education, Institute of Precision Medicine, Jining Medical University Jining 272067, China.

Cancer cells prone to utilize aerobic glycolysis other than oxidative phosphorylation to sustain its continuous cell activity in the stress microenvironment. Meanwhile, cancer cells generally suffer from genome instability, and both radiotherapy and chemotherapy may arouse DNA strand break, a common phenotype of genome instability. Glycolytic enzyme PFKFB3 (6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoform 3), plays essential roles in variety physiology and pathology processes, and generally maintain high level in cancer cells. Although this protein has been reported to involve in genome instability, its role remains unclear and controversial. Here, we showed that PFK-15, a PFKFB3 inhibitor, obviously induced apoptosis, cell viability loss, and inhibited cell proliferation/migration. Besides, PFK-15 was also found to induce necroptosis, as it not only up-regulated the phosphorylated RIP1, RIP3 and MLKL, but also enhanced the interaction between RIP3 and RIP1/MLKL, all of which are characterization of necroptosis induction. Both genetically and pharmacologically deprivation of necroptosis attenuated the cytotoxic effect of PFK-15. Besides, PFK-15 increased the γ-H2AX level and micronuclei formation, markers for genome instability, and inhibition of necroptosis attenuated these phenotypes. Collectively, the presented data demonstrated that PFK-15 induced genome instability and necroptosis, and deprivation of necroptosis attenuated cytotoxicity and genotoxicity of PFK-15 in colorectal cancer cells, thereby revealing a more intimate relationship among PFKFB3, necroptosis and genome instability.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167677PMC
May 2021

Smoking cessation, weight gain and risk of cardiovascular disease.

Heart 2021 Jun 2. Epub 2021 Jun 2.

Epidemiology and Prevention Group, Center for Public Health Sciences, National Cancer Center, Tokyo, Japan.

Objective: To examine whether the relationship between smoking cessation and risk of cardiovascular disease (CVD) was modified by weight gain.

Methods: A total of 69 910 participants (29 650 men and 46 260 women) aged 45-74 years were grouped into six groups by smoking status in the first and 5-year surveys: sustained smokers, recent quitters according to postcessation weight gain (no weight gain, 0.1-5.0 kg, >5.0 kg), long-term quitters and never smokers. Quitting smoking within and longer than 5 years were defined as recent and long-term quitters, respectively. We used Cox proportional hazard models to estimate the HR for incident CVD, coronary heart disease (CHD) and stroke.

Results: We identified 4023 CVDs (889 CHDs and 3217 strokes) during a median of 14.8 years of follow-up. Compared with sustained smokers, the multivariable HR (95% CI) for CVD was 0.66 (0.52 to 0.83) for recent quitters without weight gain, 0.71 (0.55 to 0.90) for recent quitters with weight gain of 0.1-5.0 kg, 0.70 (0.44 to 1.10) for recent quitters with weight gain of >5.0 kg, 0.56 (0.49 to 0.64) for long-term quitters, and 0.60 (0.55 to 0.66) for never smokers. The analysis restricted to men showed a similar association. Prespecified analysis by age suggested that recent quitters overall had a lower HR for CVD among those aged <60 years vs ≥60 years. Similar patterns of association were observed in CHD and stroke.

Conclusions: Postcessation weight gain did not attenuate the protective association between smoking cessation and risk of CVD.
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http://dx.doi.org/10.1136/heartjnl-2021-318972DOI Listing
June 2021

Consumption of soy products and cardiovascular mortality in people with and without cardiovascular disease: a prospective cohort study of 0.5 million individuals.

Eur J Nutr 2021 Jun 2. Epub 2021 Jun 2.

Public Health, Department of Social Medicine, Osaka University Graduate School of Medicine, Osaka, 5650871, Japan.

Purpose: We sought to examine the association of soy product consumption with risk of cardiovascular death in Chinese individuals with and without a history of cardiovascular disease (CVD).

Methods: The current analysis included 487,034 individuals free of CVD and 22,923 individuals with a history of CVD at study baseline. Data on consumption of soy products were collected by a food frequency questionnaire. The Cox regression was used to obtain the hazard ratios (HRs) of cardiovascular mortality associated with soy product consumption among people with and without a history of CVD at baseline.

Results: During the period of follow-up, 12,582 and 2860 cardiovascular deaths were recorded among people without and with a history of CVD. Compared with those who never or rarely ate soy products, the multivariable HRs (95% CIs) were 1.02 (0.96, 1.08) for those who ate soy products monthly, 1.01 (0.95, 1.07) for those who ate soy products 1-3 days per week, 0.95 (0.88, 1.04) for those who ate soy products ≥ 4 days per week. For cause-specific mortality, soy product consumption was inversely associated with mortality from acute myocardial infarction (HR [95% CI] = 0.75 [0.61, 0.92]). Among people with a history of CVD, higher soy product consumption was not associated with cardiovascular mortality.

Conclusions: Soy consumption ≥ 4 days per week was associated with a significantly lower risk of mortality from acute myocardial infarction in comparison with never or rarely consumption among people without a history of CVD. Among people with a history of CVD, higher soy product consumption was not associated with cardiovascular mortality.
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http://dx.doi.org/10.1007/s00394-021-02602-3DOI Listing
June 2021

FTRLIM: Distributed Instance Matching Framework for Large-Scale Knowledge Graph Fusion.

Entropy (Basel) 2021 May 13;23(5). Epub 2021 May 13.

School of Software Engineering, Tongji University, Shanghai 201804, China.

Instance matching is a key task in knowledge graph fusion, and it is critical to improving the efficiency of instance matching, given the increasing scale of knowledge graphs. Blocking algorithms selecting candidate instance pairs for comparison is one of the effective methods to achieve the goal. In this paper, we propose a novel blocking algorithm named MultiObJ, which constructs indexes for instances based on the Ordered Joint of Multiple Objects' features to limit the number of candidate instance pairs. Based on MultiObJ, we further propose a distributed framework named Follow-the-Regular-Leader Instance Matching (FTRLIM), which matches instances between large-scale knowledge graphs with approximately linear time complexity. FTRLIM has participated in OAEI 2019 and achieved the best matching quality with significantly efficiency. In this research, we construct three data collections based on a real-world large-scale knowledge graph. Experiment results on the constructed data collections and two real-world datasets indicate that MultiObJ and FTRLIM outperform other state-of-the-art methods.
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http://dx.doi.org/10.3390/e23050602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153108PMC
May 2021

Chlorogenic acid supplementation ameliorates hyperuricemia, relieves renal inflammation, and modulates intestinal homeostasis.

Food Funct 2021 Jun;12(12):5637-5649

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China. and Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.

Hyperuricemia (HUA) is induced by abnormal purine metabolism and elevated serum uric acid (UA) concentrations, and it is often accompanied by inflammatory responses and intestinal disorders. This study aims to assess the protective effects of chlorogenic acid (CGA) on HUA in mice. CGA or allopurinol was given to mice with HUA induced by hypoxanthine and potassium oxonate. CGA lowered the levels of UA, blood urea nitrogen (BUN), creatinine (CR), AST, and ALT; inhibited xanthine oxidase (XOD) activity; and downregulated the mRNA expression of UA secretory proteins in HUA mice. Moreover, CGA significantly reduced serum lipopolysaccharides (LPS) levels and the mRNA expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, NOD-like receptor superfamily pyrin domain containing 3 (NLRP3), and caspase-1, and it inhibited the activation of the toll-like receptor 4/myeloid differentiation factor 88/nuclear factor kappa B (TLR4/MyD88/NF-κB) signaling pathway in the kidney, resulting in inflammation relief in HUA mice. In addition, CGA treatment increased the production of fecal short-chain fatty acids (SCFAs) in HUA mice. Additional investigations showed that CGA significantly lowered the mRNA expression of ileal IL-1β and IL-6, and it increased the mRNA expression of intestinal tight junction proteins (zonula occludens-1 (ZO-1) and occludin). Also, CGA increased the relative abundance of SCFA-producing bacteria, including Bacteroides, Prevotellaceae UGC-001, and Butyricimonas, and it reversed the purine metabolism and glutamate metabolism functions of gut microbiota. In conclusion, CGA may be a potential candidate for relieving the symptoms of HUA and regulating its associated inflammatory responses and intestinal homeostasis.
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http://dx.doi.org/10.1039/d0fo03199bDOI Listing
June 2021

Does multiple sclerosis increase the risk of preeclampsia? A systematic review and meta-analysis.

Hypertens Pregnancy 2021 May 17:1-6. Epub 2021 May 17.

Public Health, Department of Social Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.

: We investigated, via systematic review and meta-analysis, whether multiple sclerosis (MS) is associated with the risk of preeclampsia (PE).: From the eligible studies, we pooled odds ratios (ORs) and confidence intervals (CIs) of PE for pregnant women with MS compared with pregnant women without it using the fixed-effects model. The measured heterogeneity between studies.: Eight eligible studies (9 cohorts) were included. Pregnant women with MS had no excess risk of PE compared with pregnant women without MS (pooled OR = 0.99, 95% CI: 0.89, 1.09;  = 0.00%).: MS is not associated with PE.
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http://dx.doi.org/10.1080/10641955.2021.1921792DOI Listing
May 2021

Integrating Population Variants and Protein Structural Analysis to Improve Clinical Genetic Diagnosis and Treatment in Nephrogenic Diabetes Insipidus.

Front Pediatr 2021 29;9:566524. Epub 2021 Apr 29.

Department of Nephrology, National Pediatric Medical Center of China, Children's Hospital of Fudan University, Shanghai, China.

Congenital nephrogenic diabetes insipidus (NDI) is a rare genetic disorder characterized by renal inability to concentrate urine. We utilized a multicenter strategy to investigate the genotype and phenotype in a cohort of Chinese children clinically diagnosed with NDI from 2014 to 2019. Ten boys from nine families were identified with mutations in or along with dehydration, polyuria-polydipsia, and severe hypernatremia. Genetic screening confirmed the diagnosis of seven additional relatives with partial or subclinical NDI. Protein structural analysis revealed a notable clustering of diagnostic mutations in the transmembrane region of and an enrichment of diagnostic mutations in the C-terminal region of . The pathogenic variants are significantly more likely to be located inside the domain compared with population variants. Through the structural analysis and prediction, the eight mutations identified in this study were presumed to be disease-causing. The most common treatments were thiazide diuretics and non-steroidal anti-inflammatory drugs (NSAIDs). Emergency treatment for hypernatremia dehydration in neonates should not use isotonic saline as a rehydration fluid. Genetic analysis presumably confirmed the diagnosis of NDI in each patient in our study. We outlined methods for the early identification of NDI through phenotype and genotype, and outlined optimized treatment strategies.
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http://dx.doi.org/10.3389/fped.2021.566524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116627PMC
April 2021

Combined healthy lifestyle and depressive symptoms: a meta-analysis of observational studies.

J Affect Disord 2021 06 26;289:144-150. Epub 2021 Apr 26.

Public Health, Department of Social Medicine, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, 565-0871, Osaka, Japan. Electronic address:

Background: To systematically summarize the association between combined lifestyle (at least three factors, including but not limited to smoking, drinking, physical activity, body mass index, diet) and depressive symptoms by a meta-analysis in general populations.

Methods: Multiple electronic databases were searched for observational studies investigating combined lifestyle factors and depressive symptoms published before September 2020. Pooled risk estimates were calculated using random-effects models. Publication bias was conducted using the Egger's and Begg's tests.

Results: A total of 12 studies with 185,899 participants were included. The pooled OR of depressive symptoms in meta-analysis of 7 cross-sectional studies was 0.53 (0.39, 0.72), I = 83.6%, P for heterogeneity <0.001 and the pooled RR was 0.33 (0.12, 0.89), I = 95.3%, P for heterogeneity <0.001 in meta-analysis of 5 cohort studies for people with the highest versus lowest score of heathy lifestyles. In sensitivity analyses, the heterogeneity was significantly reduced in cross-sectional studies (pooled OR = 0.74 [0.65, 0.85], I = 21.7%, P for heterogeneity = 0.27) and cohort studies (pooled RR = 0.53 [0.38, 0.74], I = 15.2%, P for heterogeneity = 0.32). The publication bias corrected by "trim-and-fill" analysis yielded unchanged results.

Limitations: Limitations included residual confounding in original studies, heterogeneity between studies, and potential publication bias in the analysis of cross-sectional studies.

Conclusion: The healthy lifestyle was associated with a reduced risk of depressive symptoms. Adherence to overall healthy lifestyles is essential for the primary prevention of depression in general populations.
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http://dx.doi.org/10.1016/j.jad.2021.04.030DOI Listing
June 2021
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