Publications by authors named "Xiaowei Sun"

193 Publications

Online extraction based on ionic covalent organic framework for sensitive determination of trace per- and polyfluorinated alkyl substances in seafoods by UHPLC-MS/MS.

Food Chem 2021 May 27;362:130214. Epub 2021 May 27.

Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China. Electronic address:

The ionic covalent organic framework (TPB-BFBIm-iCOF) was facilely synthetized by the size-controllable confinement method and chosen as the online solid phase extraction (SPE) adsorbent. This adsorbent showed fast adsorption equilibrium (5 min) and high adsorption capacity (87.7-140.8 mg g) for the per- and polyfluorinated alkyl substances (PFASs). The TPB-BFBIm-iCOF microsphere revealed the satisfactory enrichment performance for PFASs by means of the electrostatic interaction, hydrophobic effect and ordered channel structure. After extraction, the loaded TPB-BFBIm-iCOF-online SPE column was eluted and applied to the ultrahigh performance liquid chromatography tandem mass spectrometry analysis. Under the optimum conditions, the method displayed satisfactory linearity (R ≥ 0.9910) and low limits of detection (≤0.0017 ng g) for five seafoods. The relative recoveries of PFASs were 85.3%-109.4% with the relative standard deviation ≤ 9.9%. The method exhibited potential value in monitoring the toxicokinetics and environmental behaviors of PFASs.
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http://dx.doi.org/10.1016/j.foodchem.2021.130214DOI Listing
May 2021

Corrigendum to "Removal and tolerance mechanism of Pb by a filamentous fungus: A case study" [Chemosphere 225 (2019) 200-208].

Chemosphere 2021 Sep 24;279:130938. Epub 2021 May 24.

College of Forestry, Henan Agricultural University, Zhengzhou, 450002, China. Electronic address:

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http://dx.doi.org/10.1016/j.chemosphere.2021.130938DOI Listing
September 2021

Nattokinase Attenuates Retinal Neovascularization Via Modulation of Nrf2/HO-1 and Glial Activation.

Invest Ophthalmol Vis Sci 2021 May;62(6):25

Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China.

Purpose: Nattokinase (NK), an active ingredient extracted from traditional food Natto, has been studied for prevention and treatment of cardiovascular diseases due to various vasoprotective effects, including fibrinolytic, antihypertensive, anti-atherosclerotic, antiplatelet, and anti-inflammatory activities. Here, we reported an antineovascular effect of NK against experimental retinal neovascularization.

Methods: The inhibitory effect of NK against retinal neovascularization was evaluated using an oxygen-induced retinopathy murine model. Expressions of Nrf2/HO-1 signaling and glial activation in the NK-treated retinae were measured. We also investigated cell proliferation and migration of human umbilical vein endothelial cells (HUVECs) after NK administration.

Results: NK treatment significantly attenuated retinal neovascularization in the OIR retinae. Consistently, NK suppressed VEGF-induced cell proliferation and migration in a concentration-dependent manner in cultured vascular endothelial cells. NK ameliorated ischemic retinopathy partially via activating Nrf2/HO-1. In addition, NK orchestrated reactive gliosis and promoted microglial activation toward a reparative phenotype in ischemic retina. Treatment of NK exhibited no cell toxicity or anti-angiogenic effects in the normal retina.

Conclusions: Our results revealed the anti-angiogenic effect of NK against retinal neovascularization via modulating Nrf2/HO-1, glial activation and neuroinflammation, suggesting a promising alternative treatment strategy for retinal neovascularization.
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http://dx.doi.org/10.1167/iovs.62.6.25DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164371PMC
May 2021

Comparative Metabolomics Revealing the Metabolic Responses of Pathogenic Bacteria to Different Antibiotics.

Methods Mol Biol 2021 ;2296:367-380

Department of Human Sciences, The Ohio State University, Columbus, OH, USA.

This protocol describes the extraction and analysis of bacterial metabolites to determine the metabolic changes pertaining to their responses to different types of antibiotics. Polar metabolites are extracted using a methanol-based extraction. Sensitive, specific, and semi-quantitative metabolite analysis was performed using a high-performance liquid chromatography coupled to a high-resolution quadrupole-Orbitrap mass spectrometry. Using our example bacteria as a demonstration, 14,528 metabolic features can be detected, and 1448 metabolites were putatively identified via basic database search. Additionally, 93 metabolites can be confidently identified via high-purity standards. Statistical analysis of these metabolites can pinpoint crucial changes in metabolic states of pathogens going through antibiotic treatment, which may assist our understanding of the antibiotic mechanism of actions from a metabolic perspective.
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http://dx.doi.org/10.1007/978-1-0716-1358-0_21DOI Listing
January 2021

Integration of Metabolomics and Gene Expression Profiling Elucidates IL4I1 as Modulator of Ibrutinib Resistance in ABC-Diffuse Large B Cell Lymphoma.

Cancers (Basel) 2021 Apr 29;13(9). Epub 2021 Apr 29.

Division of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA.

Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin lymphoma (NHL). B-cell NHLs rely on Bruton's tyrosine kinase (BTK) mediated B-cell receptor signaling for survival and disease progression. However, they are often resistant to BTK inhibitors or soon acquire resistance after drug exposure resulting in the drug-tolerant form. The drug-tolerant clones proliferate faster, have increased metabolic activity, and shift to oxidative phosphorylation; however, how this metabolic programming occurs in the drug-resistant tumor is poorly understood. In this study, we explored for the first time the metabolic regulators of ibrutinib-resistant activated B-cell (ABC) DLBCL using a multi-omics analysis that integrated metabolomics (using high-resolution mass spectrometry) and transcriptomic (gene expression analysis). Overlay of the unbiased statistical analyses, genetic perturbation, and pharmaceutical inhibition was further used to identify the key players contributing to the metabolic reprogramming of the drug-resistant clone. Gene-metabolite integration revealed interleukin four induced 1 (IL4I1) at the crosstalk of two significantly altered metabolic pathways involved in producing various amino acids. We showed for the first time that drug-resistant clones undergo metabolic reprogramming towards oxidative phosphorylation and are modulated via the BTK-PI3K-AKT-IL4I1 axis. Our report shows how these cells become dependent on PI3K/AKT signaling for survival after acquiring ibrutinib resistance and shift to sustained oxidative phosphorylation; additionally, we outline the compensatory pathway that might regulate this metabolic reprogramming in the drug-resistant cells. These findings from our unbiased analyses highlight the role of metabolic reprogramming during drug resistance development. Our work demonstrates that a multi-omics approach can be a robust and impartial strategy to uncover genes and pathways that drive metabolic deregulation in cancer cells.
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http://dx.doi.org/10.3390/cancers13092146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124963PMC
April 2021

Prenatal exposure to residential PM and its chemical constituents and weight in preschool children: A longitudinal study from Shanghai, China.

Environ Int 2021 Sep 24;154:106580. Epub 2021 Apr 24.

NHC Key Lab. Of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, 779 Old Hu Min Road, Shanghai 200237, China.

Background: Studies have reported that prenatal exposure to fine particulate matter (PM) might be associated with adverse birth outcomes in offspring. However, evidence with regard to the effects of prenatal exposure to PM and, especially, its main chemical constituents on offspring's weight in childhood is limited and inconsistent.

Objectives: The present study aimed to examine associations of prenatal exposure to PM total mass and its chemical constituents in each trimester with children's weight from birth to 6 years of age using data from Shanghai-Minhang Birth Cohort Study.

Methods: A total of 1,084 mother-infant pairs were included with both PM exposure data and at least one measurement of weight and height. Weight-for-Length (WLZ), BMI-for-Age (BMIz), and Weight-for-Age (WAZ) z-scores were generated according to the World Health Organization guidelines. Exposure to PM total mass and its chemical constituents [organic carbon (OC), black carbon (BC), ammonium (NH), nitrate (NO), sulfate (SO), and soil dust (SOIL)] during pregnancy was estimated from a satellite based modelling framework. We used multiple informant model to estimate the associations of trimester-specific PM total mass and its specific constituents concentrations with WLZ/BMIz and WAZ of offspring at birth and 1, 4, and 6 years of age.

Results: In multiple informant model, we observed consistent patterns of associations between exposure to PM total mass, OC, BC, NH, NO, and SO during the 2 and 3 trimesters and decreased WLZ/BMIz and WAZ at 1, 4, and 6 years of age in boys. We observed associations between prenatal exposure to PM total mass, NH, and NO during the 1 and 2 trimesters and increased WLZ/BMIz and WAZ in girls at birth. However, there were null associations at 1 and 4 years of age and inverse associations at 6 years of age.

Conclusions: Prenatal exposure to PM total mass and its main chemical constituents was associated with decreased weight in boys from 1 to 6 years of age, with increased weight at birth and decreased weight at 6 years of age in girls. Our findings suggest that prenatal exposure to PM and its chemical constituents may have a lasting effect on offspring's weight in childhood.
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http://dx.doi.org/10.1016/j.envint.2021.106580DOI Listing
September 2021

Dietary isoflavones intake is inversely associated with non-alcoholic fatty liver disease, hyperlipidaemia and hypertension.

Int J Food Sci Nutr 2021 Apr 25:1-11. Epub 2021 Apr 25.

Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, China.

This study investigated associations between total isoflavones and their categories (daidzein, genistein, glycitein) intake and the risks for metabolic disorders. We used the data of 6786 Chinese adults from the Nutrition Health Atlas Project. We performed multiple logistic regression and restricted cubic spline models assessing the risks for metabolic disorders (non-alcoholic fatty liver disease (NAFLD), hyperlipidaemia, hypertension, diabetes and overweight/obesity) in each category of isoflavones. Higher total isoflavones, daidzein and genistein intake were inversely associated with NAFLD ( < .05). Higher total isoflavones, daidzein, genistein and glycitein intake were also inversely associated with hyperlipidaemia ( < .01) and hypertension ( < .01). Dose-response analyses revealed that total isoflavones, daidzein, genistein and glycitein intakes were associated with the risks of metabolic disorders in a nonlinear trend. In conclusion, total isoflavones, daidzein and genistein intake were inversely associated with NAFLD, hyperlipidaemia and hypertension. Glycitein was inversely associated with hyperlipidaemia and hypertension.
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http://dx.doi.org/10.1080/09637486.2021.1910630DOI Listing
April 2021

Accurate and reliable quantitation of short chain fatty acids from human feces by ultra high-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS).

J Pharm Biomed Anal 2021 Jun 6;200:114066. Epub 2021 Apr 6.

College of Education and Human Ecology, Department of Human Sciences, The Ohio State University, Columbus, OH, 43210, USA; The James Cancer Hospital & Solove Research Institute, The Ohio State University, Columbus, OH, 43210, USA. Electronic address:

Short chain fatty acids (SCFAs), generated from microbial fermentation of dietary fibers, can regulate weight, appetite and energy homeostasis. Therefore, measuring SCFAs in fecal samples is important to understand the relationship between dietary patterns, gut microbial metabolism, and their impact on host metabolism homeostasis. However, due to the chemical complexity of fecal samples and the volatility of these SCFAs, the quantitative measurements of SCFAs remain challenging. In this study, we developed an absolute quantitation method for accurate and reliable analysis of SCFAs using an UPLC-Q Exactive HRMS system. Nine C2-C6 SCFAs were first derivatized and then separated on a reversed-phase CSH C18 column, and quantitated by UPLC-HRMS with targeted-selected ion monitoring (t-SIM) mode. Our calibration plots showed high linearity (R>0.99) with high quantitation accuracy (from 91.24%-118.42%); additional analyses showed excellent precisions ranging from 1.12 % to 6.13 %, and accurate recoveries between 92.38 % and 109.65 % with relative standard deviations of 0.31 %-6.44 %. Meanwhile, the short-term stability, freeze and thaw stability, and 168 h storage stability were tested and reported from 85.07%-106.44% with RSDs 0.44%-20.00%, 98.99%-128.84% with RSDs 0.77%-19.79%, and 77.53%-104.42% with RSDs 0.92%-18.65%, respectively. Lastly, this quantitative method was applied to determine the SCFA concentrations and compositions in forty fecal samples from a group of study subjects participating in an obesity prevention trial, and a broad range of concentrations was noted for the detected SCFAs.
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http://dx.doi.org/10.1016/j.jpba.2021.114066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132305PMC
June 2021

Integrated slice-specific dynamic shimming diffusion weighted imaging (DWI) for rectal Cancer detection and characterization.

Cancer Imaging 2021 Apr 7;21(1):32. Epub 2021 Apr 7.

Department of Radiology, Peking University First Hospital, 8 XiShiKu Avenue, XiCheng District, Beijing, 100034, China.

Purpose: To compare integrated slice-specific dynamic shimming (iShim) diffusion weighted imaging (DWI) and single-shot echo-planar imaging (SS-EPI) DWI in image quality and pathological characterization of rectal cancer.

Materials And Methods: A total of 193 consecutive rectal tumor patients were enrolled for retrospective analysis. Among them, 101 patients underwent iShim-DWI (b = 0, 800, and 1600 s/mm) and 92 patients underwent SS-EPI-DWI (b = 0, and 1000 s/mm). Qualitative analyses of both DWI techniques was performed by two independent readers; including adequate fat suppression, the presence of artifacts and image quality. Quantitative analysis was performed by calculating standard deviation (SD) of the gluteus maximus, signal intensity (SI) of lesion and residual normal rectal wall, apparent diffusion coefficient (ADC) values (generated by b values of 0, 800 and 1600 s/mm for iShim-DWI, and by b values of 0 and 1000 s/mm for SS-EPI-DWI) and image quality parameters, such as signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of primary rectal tumor. For the primary rectal cancer, two pathological groups were divided according to pathological results: Group 1 (well-differentiated) and Group 2 (poorly differentiated). Statistical analyses were performed with p < 0.05 as significant difference.

Results: Compared with SS-EPI-DWI, significantly higher scores of image quality were obtained in iShim-DWI cases (P < 0.001). The SD was significantly reduced on b = 1600 s/mm images and ADC maps of iShim-DWI. Both SNR and CNR of b = 800 s/mm and b = 1600 s/mm images in iShim-DWI were higher than those of b = 1000 s/mm images in SS-EPI-DWI. In primary rectal cancer of iShim-DWI cohort, SI was significantly higher than SI in both b = 800 and 1600 s/mm images. ADC values were significantly lower in Group 2 (0.732 ± 0.08) × 10 mm/s) than those in Group 1 ((0.912 ± 0.21) × 10 mm/s). ROC analyses showed significance of ADC values and SI between the two groups.

Conclusion: iShim-DWI with b values of 0, 800 and 1600 s/mm is a promising technique of high image quality in rectal tumor imaging, and has potential ability to differentiate rectal cancer from normal wall and predicting pathological characterization.
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http://dx.doi.org/10.1186/s40644-021-00403-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028796PMC
April 2021

Discussion on the Rehabilitation of Stroke Hemiplegia Based on Interdisciplinary Combination of Medicine and Engineering.

Evid Based Complement Alternat Med 2021 17;2021:6631835. Epub 2021 Mar 17.

Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 8615-0040, China.

Interdisciplinary combinations of medicine and engineering are part of the strategic plan of many universities aiming to be world-class institutions. One area in which these interactions have been prominent is rehabilitation of stroke hemiplegia. This article reviews advances in the last five years of stroke hemiplegia rehabilitation via interdisciplinary combination of medicine and engineering. Examples of these technologies include VR, RT, mHealth, BCI, tDCS, rTMS, and TCM rehabilitation. In this article, we will summarize the latest research in these areas and discuss the advantages and disadvantages of each to examine the frontiers of interdisciplinary medicine and engineering advances.
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http://dx.doi.org/10.1155/2021/6631835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990546PMC
March 2021

Automated evaluation of tumor spheroid behavior in 3D culture using deep learning-based recognition.

Biomaterials 2021 05 22;272:120770. Epub 2021 Mar 22.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, 210096, China; Institute of Biomaterials and Medical Devices, Southeast University, Suzhou, Jiangsu, 215163, China. Electronic address:

Three-dimensional in vitro tumor models provide more physiologically relevant responses to drugs than 2D models, but the lack of proper evaluation indices and the laborious quantitation of tumor behavior in 3D have limited the use of 3D tumor models in large-scale preclinical drug screening. Here we propose two indices of 3D tumor invasiveness-the excess perimeter index (EPI) and the multiscale entropy index (MSEI)-and combine these indices with a new convolutional neural network-based algorithm for tumor spheroid boundary detection. This new algorithm for 3D tumor boundary detection and invasiveness analysis is more accurate than any other existing algorithms. We apply this spheroid monitoring and AI-based recognition technique ("SMART") to evaluating the invasiveness of tumor spheroids grown from tumor cell lines and from primary tumor cells in 3D culture.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120770DOI Listing
May 2021

[Applied value of color Doppler flow imaging in diagnosis of congenital subglottic haemangioma in infant].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Apr;35(4):321-324

Department of Otolaryngology,Qilu Children's Hospital of Shandong University,Ji'nan,250022,China.

To investigate the value of color Doppler flow imaging(CDFI) in the diagnosis of congenital subglottic hemangioma(CSH) in infants. The data of 18 children with CSH who underwent laryngeal CDFI examination were collected and analyzed retrospectively, and compared with those who underwent laryngeal ultrasound examination at the same time. The shape, size, blood flow characteristics of the tumor and its relationship with airway were observed. Eighteen cases were treated with propranolol orally. CDFI of larynx was reexamined after 1 week, 1 month and 3 months of treatment. CDFI could clearly show the location, shape, size and range of CSH in 18 cases, as well as the relationship with airway and surrounding tissues. CDFI images of CSH showed that the tumor was massive or nodular with abundant or patchy blood flow signals. Hemangioma was found in 6 cases on the right side, 8 cases on the left side, and 4 cases on both sides. CDFI can be used in the diagnosis of subglottic hemangioma. It has advantages in displaying its size, scope and relationship with airway, especially in the later treatment and follow-up.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.04.008DOI Listing
April 2021

Astragaloside IV-targeting miRNA-1 attenuates lipopolysaccharide-induced cardiac dysfunction in rats through inhibition of apoptosis and autophagy.

Life Sci 2021 Jun 24;275:119414. Epub 2021 Mar 24.

Department of Pharmacology, Jinzhou Medical University, Jinzhou, 121001, Liaoning, China. Electronic address:

Astragaloside IV (AS-IV), the major active constituent purified from Astragalus membranaceus, was previously reported to have protective effects against cardiac dysfunction. However, the underlying mechanism remains unknown. In the present study, we investigated the protective effect of AS-IV on lipopolysaccharide (LPS)-induced cardiac dysfunction and explored the potential mechanism by focusing on miRNA-1 (miR-1) at the animal and cellular levels. A series of methods were used, including echocardiography, flow cytometry, ELISA, immunofluorescence, transmission electron microscopy, RT-PCR, and western blotting. The results showed that both AS-IV and the miR-1 inhibitor improved cardiac dysfunction, reduced heart injury, inhibited apoptosis and autophagy, and regulated the expression of calcium- and mitochondrial energy metabolism-related proteins in the heart tissue of rats treated with LPS. Importantly, AS-IV downregulated the expression of miR-1 mRNA in heart tissue. All effects of AS-IV were at least partly abolished by miR-1 mimics. In the in vitro study, both AS-IV and the miR-1 inhibitor inhibited apoptosis and autophagy and regulated the expression of calcium- and mitochondrial energy metabolism-related proteins in heart cells treated with LPS. Similarly, AS-IV downregulated the expression of miR-1 mRNA in heart cells. All effects of AS-IV on cells were at least partly abolished by miR-1 mimics. Furthermore, miR-1 mimics exhibited effects similar to LPS both in animal and cellular studies. Taken together, these results suggest that AS-IV protects against LPS-induced cardiac dysfunction by inhibiting calcium-mediated apoptosis and autophagy by targeting miR-1, highlighting a new mechanism for the therapeutic effect of AS-IV on cardiac dysfunction.
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http://dx.doi.org/10.1016/j.lfs.2021.119414DOI Listing
June 2021

EA-Directing Formamidinium-Based Perovskite Microwires with A-Site Doping.

ACS Omega 2021 Mar 8;6(10):7157-7164. Epub 2021 Mar 8.

School of Science and Engineering and Shenzhen Key Lab of Semiconductor Lasers, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, China.

One recent development to improve optoelectronic properties of perovskites is to use a larger cation for multication engineering. The chain-like ethylammonium (EA) [(CH)NH] cation is more likely to form a one-dimensional perovskite structure; however, there is no remarkable evidence in this connection. Therefore, in this work, for the first time, the EA cation as an alternative cation was introduced into FAPbBr cubic crystals to explore the stabilities and optoelectronic properties of mixed FA EAPbBr perovskites. The results indicate that replacing FA with EA is a more effective way to realize band gap tuning and morphology transformation between the cubic shape and microwires. The tuned band gap of perovskite is due to the variation of Pb-Br-Pb angles induced by the insertion of the larger EA cation. We highlight that this work provides new physical insights into the correlation between the engineering of organic cations and the formation of perovskite microwires and the tunable band gap. This observation will help us to find new ways to grow perovskite microwires and subsequently study the optoelectronic performance of low-dimensional perovskites devices.
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http://dx.doi.org/10.1021/acsomega.1c00213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970562PMC
March 2021

Application of Comparative Lipidomics to Elucidate Postprandial Metabolic Excursions Following Dairy Milk Ingestion in Individuals with Prediabetes.

J Proteome Res 2021 05 15;20(5):2583-2595. Epub 2021 Mar 15.

Human Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, Ohio 43210, United States.

Nutrient-dense dairy foods are an important component of a healthy diet. Recommendations, however, advise non- and low-fat dairy foods despite controversy concerning whether full-fat dairy foods adversely impact cardiometabolic health. Therefore, in this study, our objective is to examine the differential plasma lipidomic responses to non-fat or full-fat milk ingestion during postprandial hyperglycemia. Seven adults with prediabetes completed a randomized cross-over study in which glucose was consumed alone or with non-fat or full-fat dairy milk. Plasma samples collected at 90 min and 180 min post milk ingestion were used to perform untargeted lipidomics analysis. A total of 332 lipids from 20 classes and five lipid categories were detected at different time points during the postprandial period. Dairy milk, especially non-fat milk, protected against lipid changes otherwise induced by glucose ingestion. Co-ingestion of dairy milk with glucose, regardless of fat content, significantly altered lipid profiles although full-fat milk more substantially modulated lipid profiles. For the identified lipid biomarkers, 68.0% and 66.7% of the lipids significantly increased at 90 and 180 min, respectively, while phosphatidylcholines (GPs) contributed most for the significant increase. Comparative lipidomics analysis indicated that both types of dairy milk induced significant changes in several lipid pathways, including glycerophospholipid metabolism and α-linolenic acid metabolism, to protect against postprandial hyperglycemia. In summary, our comparative lipidomics results suggested that dairy milk-mediated lipid modulation may be an effective dietary approach to reduce the risk of metabolic diseases among those with prediabetes.
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http://dx.doi.org/10.1021/acs.jproteome.0c01009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106868PMC
May 2021

Efficacy of Shenling Baizhu San on stable chronic obstructive pulmonary disease patients: A systematic review and meta-analysis.

J Ethnopharmacol 2021 May 16;272:113927. Epub 2021 Feb 16.

Reproductive Health Center, Women and Children's Hospital, Qingdao University, Qingdao, China. Electronic address:

Ethnopharmacological Relevance: Shenling Baizhu San (SBS) as a classic Chinese medicine prescription, has been extensively used in gastrointestinal diseases, such as ulcerative colitis and chronic diarrhea. In recent years, SBS has shown a beneficial effect on chronic obstructive pulmonary disease (COPD) patients. However, clinical trials had shown conflicting results of SBS on improving pulmonary function and other related indicators of patients with stable COPD. The efficacy of SBS on stable COPD patients has not been fully assessed.

Aim Of The Study: To determine whether the SBS used in the treatment of gastrointestinal disease was effective to treat COPD, we assessed the clinical evidence and efficacy of SBS supplemental treatment on stable COPD patients by a systematic review and meta-analysis of clinical trials.

Materials And Methods: Nine electronic databases were searched to include clinical trials (published until August 31, 2020) with SBS as a supplementation treatment on stable COPD. Mean difference (MD) was used to evaluate continuous variables, odds ratio (OR) was calculated to evaluate dichotomous. The Egger's test was applied for publication bias.

Results: A total of 770 COPD participants from 11 trials that met the inclusion criteria were included. The meta-analysis showed that modified SBS could improve the exercise endurance, life quality scores of stable COPD patients, and also showed the potential benefits to pulmonary function of COPD patients than original SBS.

Conclusion: The methodological quality of included trials may limit the conclusions that indicate that modified SBS may have a promising treatment for improving FEV/FVC and MVV, increasing exercise endurance and life quality scores on stable COPD patients.
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http://dx.doi.org/10.1016/j.jep.2021.113927DOI Listing
May 2021

Efficacy and safety of drug-eluting bead-transcatheter arterial chemoembolization using 100-300 μm versus 300-500 μm CalliSpheres microspheres in patients with advanced-stage hepatocellular carcinoma.

J Cancer Res Ther 2020 ;16(7):1582-1587

Qingdao Center for Disease Control and Pervention, Qingdao, China.

Purpose: To evaluate the efficacy and safety of drug-eluting bead-transcatheter arterial chemoembolization (DEB-TACE) using 100-300 μm versus 300-500 μm CalliSpheres microspheres (CSMs) for treating multiple hepatocellular carcinoma (HCC) with the largest nodule measuring between 5 and 10 cm.

Methods: Eighty-one advanced-stage HCC patients treated by DEB-TACE between January 2017 and March 2020 were retrospectively analyzed. There were 48 patients in the 100-300 μm group and 33 patients in the 300-500 μm group. Treatment response, liver function tests, and complications were compared between the two groups.

Results: At 6 months, the response rates in the 100-300 μm group were higher than those in the 300-500 μm group (P < 0.05). Disease control rates in the 100-300 μm group were higher than those in the 300-500 μm group at 1, 3, and 6 months (P < 0.05). Progression-free survival (PFS) and overall survival (OS) were longer in the 100-300 μm group (P < 0.05). Visual analog score points were higher in the 300-500 μm group (P < 0.05). There were no significant differences in complications between the two groups.

Conclusion: The use of small-diameter CSMs in multiple HCC with the largest nodule size of 5-10 cm provides better treatment response and longer PFS and OS for treating advanced-stage HCC.
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http://dx.doi.org/10.4103/jcrt.JCRT_543_20DOI Listing
January 2020

What is the ideal route of administration of tranexamic acid in total knee arthroplasty? A meta-analysis based on randomized controlled trials.

Ann Palliat Med 2021 Feb 15;10(2):1880-1894. Epub 2021 Jan 15.

Department of Orthopedic Surgery, Beijing Key Lab Immune-Mediated Inflammatory Diseases, China-Japan Friendship Hospital, Peking Union Medical School, Beijing, China.

Background: Tranexamic acid (TXA) was conducive in total knee arthroplasty (TKA) to reduce blood loss and transfusion demand. The purpose of this meta-analysis was to assess the efficacy and safety of different administration of TXA in primary TKA.

Methods: Database PubMed, Medline, Web of Science and Embase were searched. The relative risks (RRs) with 95% confidence intervals (CIs) were calculated to analysis dichotomous outcomes. The mean differences (MD) with 95% CIs were calculated to analysis dichotomous outcomes. Data was analyzed using RevMan 5.3.

Results: Twenty-eight randomized controlled trials (RCTs) studies were included in this meta-analysis involving a total of 4,200 participants. There were no obvious differences between oral, intravenous or topical TXA group in total blood loss (intravenous vs. topical: MD =11.55, 95% CI, -10.23 to 33.34, oral vs. intravenous or topical: MD =-52.25, 95% CI, -121.28 to 16.78), transfusion rate (intravenous vs. topical: RR =1.04, 95%CI, 0.64 to 1.69, oral vs. intravenous or topical: RR =0.75, 95% CI, 0.36 to 1.54), incidence of venous thrombotic events (VTE) (intravenous vs. topical: RR =1.43, 95% CI, 0.81 to 2.54). The topical TXA administration had significantly increased postoperative hemoglobin (HB) level compared with the intravenous TXA administration (MD =-0.37, 95% CIs, -0.47 to -0.26). In the combined group, the total blood loss (MD =-119.58, 95% CI, -181.68 to -57.49) and postoperative HB level (MD =0.54, 95% CI, 0.45 to 0.64) were more acceptable than the single-route group.

Conclusions: Combined administration of TXA can reduce total blood loss, postoperative HB drop compared with intravenous, topical or oral TXA alone. Oral administration of TXA is similar to intravenous or topical TXA use alone.
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http://dx.doi.org/10.21037/apm-20-1857DOI Listing
February 2021

Anthropometry of the medial femoral condyle in the Chinese population: the morphometric analysis to design unicomparmental knee component.

BMC Musculoskelet Disord 2021 Jan 20;22(1):95. Epub 2021 Jan 20.

Department of Orthopedic Surgery, Beijing Key Lab Immune-Mediated Inflammatory Diseases, China-Japan Friendship Hospital, Peking Union Medical School, 100029, Beijing, China.

Background: This study aimed to assess the radii of the distal and posterior articular surfaces of the medial femoral condyle in a Chinese population and provide detailed parameters of the knee joint for the future design of UKA components.

Methods: This study included 500 consecutive Chinese patients who underwent knee MRI from Jan 2019 to Jan 2020. The two most appropriate circles were used to reveal the distal and posterior joint surfaces in the sagittal plane of the MRI images. The radius of the circle representing the distal articular surface in the sagittal plane was measured as R1, and the radius of the posterior articular surface was measured as R2. The distance between the centers of the two rotation circles was recorded as d. An independent t test was used to compare the differences between men and women. The Pearson correlation coefficient was calculated to analyze the correlation between R1 and R2. SPSS v19.0 software was used for statistical analysis.

Results: The average values of R1, R2, R1/R2 and d were calculated. Scatter plots were constructed to show the trend of changes in the radius of the distal and posterior articular surfaces of the femoral condyle. R1, R2 and d differed significantly between men and women (p < 0.05). Correlation analysis showed that R1 was positively correlated with R2 (r = 0.61, p < 0.05).

Conclusions: The data of the radii of the distal and posterior articular surfaces of the medial femoral condyle were provided. In the UKA design, the relationships between the radii of the distal and posterior articular surfaces should be taken into account.
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http://dx.doi.org/10.1186/s12891-021-03979-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819177PMC
January 2021

An investigation on gastric cancer staging using CT structured report.

Eur J Radiol 2021 Mar 13;136:109550. Epub 2021 Jan 13.

Department of Radiology, Peking University First Hospital, Beijing, China. Electronic address:

Objective: To evaluate the clinical performance of structured report (SR) for CT in patients with pre-operative staging of gastric cancer, compared to non-SR.

Methods: Retrospectively, 51 consecutive cases with primary gastric cancer staging were enrolled. Every SR or non-SR was performed by two GI radiologists (1 junior and 1 senior). Interobserver agreement was conducted between the junior and senior groups for both SR and non-SR. 10 key features required for lesion description and staging were assessed between SR and non-SR. Diagnostic content between SR and non-SR was also compared. Accuracy of SR and non-SR on T staging was measured. Subjective evaluation of SR vs. non-SR was also conducted in form of survey by 20 radiologists and 3 GI surgeons.

Results: Interobserver agreement showed excellent in SR (Kappa = 1, P < 0.001), but poor in non-SR (Kappa = 0.036, P = 0.455). For the 10 key features required for lesion assessment, non-SR showed 6.84 ± 0.83 while SR reported all of them (P < 0.001). Statistically significant improvement was observed in the SR for parts of key features, especially for assessment of adjacent organs and vessels (P < 0.001). Accuracy comparison of T staging showed higher in SR for cohort of T4a (P = 0.028<0.05). The scores of subjective evaluation were higher (P < 0.05) in SR than in non-SR by both radiologists and surgeons. Meanwhile, the inter-observer agreement among surgeons was good in SR with significance (w=0.53, P = 0.005 for efficiency; w=0.638, P < 0.001 for integrity) but poor in non-SR.

Conclusions: SR of gastric multiphasic CT ensured reliable detection of all relevant key features for staging along with reproducible documentation, which was not always the case for non-SR. In addition, SR has the potential in improving diagnostical accuracy of T staging and was welcomed by clinicians.
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http://dx.doi.org/10.1016/j.ejrad.2021.109550DOI Listing
March 2021

Study on Development of Composite Hydrogels With Tunable Structures and Properties for Tumor-on-a-Chip Research.

Front Bioeng Biotechnol 2020 22;8:611796. Epub 2020 Dec 22.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.

A major factor for developing new tumor models is to recreate a proper three-dimensional environment for 3D tumors culture. In this 3D microenvironment, extracellular matrices play important roles in regulation of hallmark features of cancer through biochemical and mechanical signals. The fabrication of a mechanical and biophysical controllable hydrogel, while sharing similarities with Matrigel in cancer invasiveness evaluation, is an urgent but unmet need. In this study, we developed a hybrid hydrogel system composed of GelMA and hydrolyzed collagen to model tumor micro-environment and tested with several cancer cells with different origin and characteristics. This hydrogel possesses a well-ordered homogenous microstructure, excellent permeability and an adjustable mechanical stiffness. This hydrogel demonstrated similar properties as Matrigel in tumor spheroids culture and 3D tumor invasiveness studies. It was further applied in a Tumor-on-a-Chip system with 3D-bioprinting. Our research demonstrated this hydrogel's effectiveness in tumor 3D culture, and its potential to replace Matrigel in cancer invasiveness evaluation.
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http://dx.doi.org/10.3389/fbioe.2020.611796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786431PMC
December 2020

Bearing dislocation of mobile bearing unicompartmental knee arthroplasty in East Asian countries: a systematic review with meta-analysis.

J Orthop Surg Res 2021 Jan 7;16(1):28. Epub 2021 Jan 7.

Department of Orthopaedic Surgery, China-Japan Friendship Hospital, No. 2, Yinghuadong Road, Chaoyang District, Beijing, 100029, China.

Background: Bearing dislocation is a common postoperative complication of mobile-bearing unicompartmental knee arthroplasty, and East Asian patients tend to be at higher risk. However, whether this high dislocation rate is common in all East Asian populations remains unclear. This meta-analysis was performed to explore this issue and describe various features of dislocation in East Asians, including the onset time, dislocation direction, and re-dislocation rate.

Methods: The literature was searched in PubMed, Embase, Ovid, and Cochrane Library up to May 2020. Studies were scrutinized by two independent authors, and the bearing dislocation rate, onset time, direction, and re-dislocation rate were specifically analyzed. RevMan 5.3 was used for the statistical analysis.

Results: Seven case series from Korea, China, and Japan were included. The pooled analysis showed that the total dislocation rate was 2.37%, while the subgroup analysis showed that the dislocation rate in Korea and other countries was 4.50% and 0.74%, respectively (P < 0.01). Another subgroup analysis of the onset time showed a significant difference before and after the first 5 years postoperatively (P < 0.01). Anterior and posterior dislocations were more frequent than medial and lateral dislocations (P < 0.01). The average re-dislocation rate was 32.45%, which was approximately seven times higher than the primary dislocation rate (P < 0.01).

Conclusion: Our meta-analysis demonstrated that Korea had a higher bearing dislocation rate among East Asian countries, especially in the first 5 years after primary UKA. Anterior and posterior dislocations were common. The most important finding is that the re-dislocation rate can be much higher than the initial dislocation rate.
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http://dx.doi.org/10.1186/s13018-020-02190-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791981PMC
January 2021

Ferulic acid attenuates microglia-mediated neuroinflammation in retinal degeneration.

BMC Ophthalmol 2021 Jan 6;21(1):13. Epub 2021 Jan 6.

Department of Ophthalmology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, 264000, People's Republic of China.

Background: Retinal degeneration is often accompanied by microglia-mediated neuroinflammation. Ferulic acid (FA), an active ingredient of traditional Chinese medicines (TCMs), has been reported to have anti-inflammatory effects. This study explores the impact of FA on microglia-mediated neuroinflammation and associated retinal degeneration in rd10 mice.

Methods: Rd10 mice received different concentrations of FA every day from postnatal day (P)4 to P24. On P25, the visual function of the mice was evaluated by electroretinogram, and retinae were collected for further investigation. Microglial activation and the expression of relevant cytokines in the retina were evaluated by qPCR, western blotting and immunofluorescence staining. Retinal structure was assessed by haematoxylin and eosin (HE) staining.

Results: Supplementation with 50 mg/kg FA provided optimal protection against retinal degeneration, with treated mice exhibiting more photoreceptor nuclei as well as greater wave amplitude amplification on electroretinogram than untreated mice. FA suppressed microglial activation both in vivo and in vitro, and inhibited the expression of pro-inflammatory factors Tnfα, IL1β, and Ccl2 in the retinae of rd10 mice. Furthermore, FA suppressed the activation of STAT1 and subsequently inhibited IRF8 expression, potentially highlighting a role for these pathways in FA-mediated immunomodulatory activity.

Conclusions: Attenuation of neuroinflammation by FA may be beneficial for retarding retinal degeneration.
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http://dx.doi.org/10.1186/s12886-020-01765-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789661PMC
January 2021

Exosomes derived from BDNF-expressing 293T attenuate ischemic retinal injury and .

Aging (Albany NY) 2020 Nov 29;12. Epub 2020 Nov 29.

Department of Opthalmology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.

Retinal ischemia emerges in many ocular diseases and is a leading cause of neuronal death and dysfunction, resulting in irreversible visual impairment. We previously reported that brain-derived neurotrophic factor (BDNF)-expressing human 293T cells could steadily express BDNF and play a protective role in ARPE-19 cells, a human retinal epithelial cell line. Thus, we hypothesized that exosomes might be essential in the interaction between BDNF-expressing 293T cells and recipient cells. The study investigated whether exosomes derived from BDNF-expressing 293T cells (293T-Exo) can be internalized by ischemic retinal cells and exert neuroprotective roles. The results demonstrated that 293T-Exo significantly attenuated the loss of cell proliferation and cell death in R28 cells in response to oxygen-glucose deprivation treatment. Mechanistic studies revealed that the endocytosis of 293T-Exo by R28 cells displayed dose- and temperature-dependent patterns and may be mediated by the caveolar endocytic pathway via the integrin receptor. In the retinal ischemia rat model, the administration of 293T-Exo into the vitreous humor of ischemic eyes reduced apoptosis in the retina. Furthermore, 293T-Exo was mainly taken up by retinal neurons and retinal ganglion cells. Together, the results demonstrated that 293T-Exo has a neuroprotective effect in retinal ischemia and has therapeutic potential for retinal disorders.
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http://dx.doi.org/10.18632/aging.202245DOI Listing
November 2020

Prenatal exposure to perfluoroalkyl substances and thyroid hormone concentrations in cord plasma in a Chinese birth cohort.

Environ Health 2020 11 26;19(1):127. Epub 2020 Nov 26.

Department of Social Medicine and Reproductive Epidemiology, National Health Commission Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, #779 Lao Hu Min Road, Shanghai, 200032, China.

Background: Evidence of associations between prenatal exposure to perfluoroalkyl substances (PFASs) and fetal thyroid hormones (THs) is controversial, and few studies have estimated the associations, while addressing the high correlations among multiple PFASs. We aimed to examine the associations between prenatal PFAS exposure and thyroid hormone concentrations in cord blood.

Methods: A total of 300 mother-infant pairs from the Shanghai-Minhang Birth Cohort Study were included. We measured the concentrations of eight PFASs in maternal plasma samples collected at 12-16 gestational weeks, as well as those of total thyroxine (T4), free T4 (FT4), total triiodothyronine (T3), free T3 (FT3), and thyroid stimulating hormone (TSH) in cord plasma. We estimated the associations between maternal PFAS concentrations and TH concentrations using linear regression and Bayesian kernel machine regression (BKMR) models.

Results: In BKMR models, higher PFAS mixture concentrations were associated with increased T3 concentrations, and there were suggestive associations with increased FT3 concentrations. For single-exposure effects in BKMR models, a change in PFDA, PFUdA, and PFOA concentrations from the 25th to 75th percentile was associated with a 0.04 (95%CrI: - 0.01, 0.09), 0.02 (95%CrI: - 0.03, 0.07), and 0.03 (95%CrI: - 0.001, 0.06) nmol/L increase in T3 concentrations, respectively. PFOA, PFNA, and PFDA were the predominant compounds in PFASs-FT3 associations, and the corresponding estimates were 0.11 (95% CrI: 0.02, 0.19), - 0.17 (95% CrI: - 0.28, - 0.07), and 0.12 (95% CrI: - 0.004, 0.24) pmol/L, respectively. A change in PFNA and PFOA concentrations from the 25th to 75th percentile was associated with a - 1.69 (95% CrI: - 2.98, - 0.41) μIU/mL decrease and a 1.51 (95% CrI: 0.48, 2.55) μIU/mL increase in TSH concentrations. The associations of PFOA and PFNA with T3/FT3 were more pronounced in boys, while those with TSH were more pronounced in girls.

Conclusion: Our results suggest that prenatal exposure to multiple PFASs was associated with thyroid hormones in cord blood. However, individual PFAS had varied effects-differing in magnitude and direction-on fetal thyroid hormones.
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http://dx.doi.org/10.1186/s12940-020-00679-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690128PMC
November 2020

DRG-Oriented Mathematical Calculation Model and Method of Integrated Medical Service Cost.

Authors:
Xiaowei Sun Yi Zhu

Comput Math Methods Med 2020 1;2020:3189676. Epub 2020 Nov 1.

Jinling Hospital Department of Pharmacy, Nanjing University, School of Medical, Nanjing, 210002 Jiangsu, China.

In the context of the new round of medical and health reform, in order to alleviate the problem of "difficult to see a doctor and expensive to see a doctor," the state focuses on reducing the cost of medical services, so it puts forward the calculation and method research of medical costs. The purpose of this study is to calculate and predict the cost of medical services in a DRG-oriented integrated environment. In this study, activity-based costing and weighted moving average methods are used. First, basic data of medical services are collected, then all medical activities are confirmed and all service costs are collected, then a cost database is established, and a calculation model of medical costs is designed. Finally, calculation suggestions and optimization methods are put forward by analyzing the calculated data. The experimental results show that the actual demand of drugs predicted by the general moving average method is relatively insufficient, with the maximum error of 41%, the minimum of 5%, and the average error of 19.8%; the maximum error of drug demand predicted by the weighted moving average method is 24%, the minimum is 2%, and the average is 15.4%. It can be concluded that the prediction effect of the weighted moving average method is better than that of the ordinary moving average method, which plays a good and effective role in the prediction of medical cost. The activity-based costing method is more detailed and organized for the cost calculation and classification of medical services. It provides a certain value for the effective management and control of medical service cost.
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http://dx.doi.org/10.1155/2020/3189676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652627PMC
November 2020

Associations of maternal soy product consumption and urinary isoflavone concentrations with neonatal anthropometry: A prospective cohort study.

Environ Pollut 2021 Apr 10;274:115752. Epub 2020 Oct 10.

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, China.

Isoflavones (ISOs) are naturally occurring endocrine-disrupting compounds. Few human studies have evaluated the effects of ISO exposure on neonatal anthropometry. This study aimed to examine the associations of maternal soy product consumption and urinary ISO concentrations, including genistein, daidzein, glycitein, and equol, with neonatal anthropometry, based on a Chinese cohort study. In Shanghai-Minhang Birth Cohort Study, pregnant women at 12-16 weeks of gestation were recruited, and they completed a structured questionnaire to assess soy product consumption during pregnancy. They also provided a single spot urine sample for the ISO assay. Neonatal anthropometric indices (birth weight; arm, waist, and head circumference; and triceps, back, and abdominal skinfold thickness) were measured at birth. Multivariable linear regression analysis was performed among the 1188 mother-infant pairs to examine the associations between maternal soy product consumption and neonatal anthropometry. The same statistical model was applied to examine the associations between maternal ISO exposure and neonatal anthropometry among 480 mother-infant pairs. Neonate girls born to mothers who "sometimes" and "frequent" consumed soy products had 169.1 g (95% confidence interval [CI], -68.9-407.1) and 256.5 g (95% CI, 17.1-495.8) higher birth weight, respectively, than those born to mothers who "never" consumed soy products during pregnancy. We observed consistent associations between higher maternal urine ISO concentrations and increased anthropometric indices (birth weight, arm and waist circumference, and triceps and abdominal skinfold thickness) in neonate girls, while no association was observed among boys. The findings suggested that maternal dietary ISO intake during pregnancy is associated with fetal development in a sex-specific pattern. In addition, follow-up studies are required to evaluate whether the observed changes in anthropometric indices at birth are associated with health conditions later in life.
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http://dx.doi.org/10.1016/j.envpol.2020.115752DOI Listing
April 2021

Cancer cells invasion to the gastric bare area adipose tissue: a poor prognostic predictor for gastric cancer.

World J Surg Oncol 2020 Nov 13;18(1):300. Epub 2020 Nov 13.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Background: The relationship between gastric bare area adipose tissues invasion (GBAI) confirmed pathologically and the prognosis of gastric cancer (GC) patients is undefined. Till present, there has not been literature investigating this phenomenon. Here, we aimed at analyzing the implication of GBAI in GC.

Methods: The data of 1822 patients who underwent radical surgery between January 2000 and December 2013 at the Sun Yat-sen University Cancer Center were retrieved. Pathologically, tumor deposits (TDs) located > 5 mm from the leading edge of the primary tumor and the lymph nodes (LNs) station number 1, 2, 7, and 9 were considered GBAI. Kaplan-Meier method, log-rank test, and Cox's proportional hazards model were employed to analyze.

Results: Two hundred and five (11.3%) patients were pathologically diagnosed with GBAI, which was more commonly found in proximal or linitis lastica than distal GC (P < 0.001). There was significant difference in 5-year survival between patients with and without GBAI for stages IIB, IIIA, IIIB, and IIIC, respectively (P < 0.009 for IIB, IIIA, and IIIB; P = 0.021 for IIIC). Among the 205 GBAI patients, 61 had detailed radiological follow-up data in which 26 (34.7%) were found to have retroperitoneal infiltration, 27 (36.0%) had peritoneal metastasis, 10 (13.3%) had hematogenous metastasis, 16 (21.3%) had lymphatic metastasis, and 16 (21.3%) had others.

Conclusions: GBAI was identified as a predictor of unfavorable prognosis for GC and was more commonly found in the proximal or linitis plastica of the stomach than in distal stomach. Retroperitoneal infiltration was one of the most commonly identified metastatic route for GC associated with GBAI after radical surgery.
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http://dx.doi.org/10.1186/s12957-020-02066-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666447PMC
November 2020

Acupuncture protects against cerebral ischemia-reperfusion injury via suppressing endoplasmic reticulum stress-mediated autophagy and apoptosis.

Mol Med 2020 11 10;26(1):105. Epub 2020 Nov 10.

Department of Orthopaedics and Traumatology, The First Affiliated Hospital, Heilongjiang University of Chinese Medicine, 26 Heping Road, Harbin, 150040, Heilongjiang, People's Republic of China.

Background: Acupuncture treatment possesses the neuroprotection potential to attenuate cerebral ischemia-reperfusion (I/R) injury. Endoplasmic reticulum (ER) stress has been suggested to be involved in the pathogenic mechanism of cerebral I/R injury. Whether acupuncture protects against cerebral I/R injury via regulating ER stress remains unclear. This study aimed to evaluate the role of ER stress in the neuroprotection of acupuncture against cerebral I/R injury and its underlying mechanisms.

Methods: Cerebral I/R injury was induced by middle cerebral artery occlusion (MCAO) in rats. Acupuncture was carried out at Baihui (GV 20), and Qubin (GB7) acupoints in rats immediately after reperfusion. The infarct volumes, neurological score, ER stress, autophagy and apoptosis were determined.

Results: Acupuncture treatment decreased infarct volume, neurological score and suppressed ER stress via inactivation of ATF-6, PERK, and IRE1 pathways in MCAO rats. Attributing to ER stress suppression, 4-PBA (ER stress inhibitor) promoted the beneficial effect of acupuncture against cerebral I/R injury. Whereas, ER stress activator tunicamycin significantly counteracted the neuroprotective effects of acupuncture. In addition, acupuncture restrained autophagy via regulating ER stress in MCAO rats. Finally, ER stress took part in the neuroprotective effect of acupuncture against apoptosis in cerebral I/R injury.

Conclusions: Our findings suggest that acupuncture offers neuroprotection against cerebral I/R injury, which is attributed to repressing ER stress-mediated autophagy and apoptosis.
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http://dx.doi.org/10.1186/s10020-020-00236-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653860PMC
November 2020

Platelet-rich plasma for the treatment of burn wounds: A meta-analysis of randomized controlled trials.

Transfus Apher Sci 2021 Feb 16;60(1):102964. Epub 2020 Oct 16.

Department of Blood Transfusion, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China. Electronic address:

Background: To evaluate the efficacy and safety of platelet-rich plasma in the treatment of burn wounds through a meta-analysis of randomized controlled trials.

Methods: We conducted a comprehensive study from electronic medical journal databases. The primary outcome was healing rate, and the secondary outcomes were healing time, adverse events, pain score and scar score. The data was analyzed using Review Manager 5.3 and Stata 12. The odds ratio (OR) among different groups was calculated by using 95 % confidence interval (CI).

Results: We included 8 randomized controlled trials with a total of 539 patients. The results showed that platelet-rich plasma could improve the healing rate of burn wound (OR 4.43, 95 % CI 2.13-9.22). The wound healing time of the platelet-rich plasma treatment group was significantly shorter than that of the conventional treatment group (OR -4.23, 95 % CI -5.48 to -2.98), both the superficial burn (OR -3.80, 95 % CI -4.53 to -3.07) and the deep burn group (OR -4.65, 95 % CI -6.90 to -2.40) had shorter healing time. Otherwise, the incidences of adverse events (OR 0.30, 95 % CI 0.11-0.78), pain score (OR -0.80, 95 % CI -1.40 to -0.21) and scar score (OR -0.38, 95 % CI -0.69 to -0.07) were all better in the platelet rich plasma treatment group.

Conclusion: Topical platelet-rich plasma treatment on burn wounds can improve wound healing and reduce the incidence of adverse events. Further research is needed to standardize the preparation and use of platelet-rich plasma and to evaluate the long-term clinical outcome of platelet-rich plasma in the treatment of burn wounds.
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http://dx.doi.org/10.1016/j.transci.2020.102964DOI Listing
February 2021